糖尿病大鼠下颌骨成骨细胞体外特性的研究

目的：研究体外培养糖尿病大鼠下颌骨成骨细胞生长分化的特性。方法：20只6周龄雌性SD大鼠随机平均分为2组，实验组腹腔注射四氧嘧啶（200mg／kg），对照组注射生理盐水。模型建立成功10d后，取下颌骨行成骨细胞培养。MTT法、PNPP法、考马斯亮蓝法、放射免疫法及钙结节茜素红染色检测细胞的增殖能力、蛋白校正ALP含量、骨钙素水平及矿化能力。结果：实验组2d、4d、6d MTT相对比值高于对照组（P〈0．05），3d、7d、14d、21d ALP含量低于对照组（P〈0．01），28d总骨钙素水平以及钙结节的数量及面积均低于对照组（P〈0．01）。结论：急性糖尿病早期促进体外培养大鼠下颌骨成骨细胞的增殖，抑制其基质成熟和矿化，可建成具有内在缺陷的成骨细胞模型。     

BMP-2与TNF-α对成骨细胞增殖和分化作用的比较分析

目的 通过BMP-2及TNF-α分别刺激原代培养的成骨细胞,探讨BMP-2及TNF-α对成骨细胞增殖和分化不同作用并进行比较分析。方法 在无菌条件下手术切取Wistar（1周龄）大鼠颅骨并提取成骨细胞,原代细胞培养、传代、纯化、扩增并进行鉴定。采用BMP-2及TNF-α进行诱导,观察成骨细胞增殖,用利用MTT法检测其变化,利用PNPP偶氮法（4-硝基苯基磷酸二钠盐）检测成骨细胞碱性磷酸酶（ALP）活性。结果 通过ALP染色和钙化的细胞实验均为阳性,证明体外培养扩增的细胞具有典型的成熟成骨细胞的特征。采用BMP-2及TNF-α来诱导大鼠成骨细胞与对照组的差异有统计学意义（P＜0.05）。细胞增殖活性及ALP活性随BMP-2浓度的升高而增加,而随TNF-α浓度的升高而降低。结论 BMP-2转染后的成骨细胞增殖和分化功能都有所提高。而TNF-α对成骨细胞的增殖和分化均有抑制作用。对体外培养的成骨细胞,BMP-2和TNF-α分别起到促进和抑制作用。     

肿瘤坏死因子-α对大鼠成骨细胞生长影响的实验研究

目的通过观察肿瘤坏死因子-α（TNF-α）对大鼠成骨细胞生长的影响,探讨TNF-α对成骨细胞的分子生物学调控作用。方法采用不同质量浓度TNF-α诱导大鼠成骨细胞,利用MTT法检测成骨细胞增殖的变化,利用4-硝基苯基磷酸二钠盐（PNPP）偶氮法检测成骨细胞碱性磷酸酶（ALP）活性情况。结果大鼠成骨细胞增殖活性及ALP活性随TNF-α质量浓度的升高而降低,其中当TNF-α质量浓度大于50 ng/mL时,与对照组的差异有统计学意义（P＜0.05）。结论TNF-α对成骨细胞的增殖和分化均有抑制作用,当TNF-α质量浓度大于50 ng/mL时,这种抑制作用更明显。     

Bio-oss骨粉对大鼠成骨细胞体外成骨效能的影响

目的 研究大鼠成骨细胞与。Bio-oss骨粉体外复合培养对成骨细胞成骨效能的影响，探讨Bio-oss作为骨组织工程支架材料的组织相容性。方法 采用原代骨髓基质干细胞(MSCs)培养技术及定向诱导培养技术获得大鼠成骨细胞，分为实验组与对照组。实验组细胞与Bio-oss骨粉复合培养，对照组细胞单独培养。比较两组成骨细胞形态学特点、碱性磷酸酶(ALP)活性、贴壁率、增殖活力及蛋白含量。结果 成骨细胞与Bio-oss骨粉复合培养后，细胞形态、增殖活力、成骨效能(ALP活性、蛋白含量)与单纯成骨细胞培养无明显差异。结论 Bio-oss骨粉能满意模拟天然骨支架，可作为骨组织工程中较理想的支架材料。     

不同直径羟基磷灰石纳米颗粒对成骨细胞代谢的影响

目的：比较喷涂法制备的羟基磷灰石（HA）涂层表面2种不同直径纳米颗粒对成骨细胞增殖活性及功能代谢的影响.方法：将体外培养的乳鼠颅骨成骨细胞分别接种于具有2种直径范围纳米颗粒的HA涂层表面（Ⅰ组：20～40 nm,Ⅱ组：70～100 nm）,并以无纳米颗粒HA涂层作对照,扫描电镜下观察涂层表面形态以及细胞贴附情况;采用MTT法、PNPP法和考马斯亮蓝蛋白含量检测法分别检测细胞的增殖活性、碱性磷酸酶（alkaline phosphatase,ALP）活性和细胞总蛋白含量,应用SPSS10.0统计软件包对数据进行单因素方差分析的q检验（SNK）.结果：细胞在HA涂层表面生长良好,MTT法测量显示,6d和8d时,Ⅰ组和Ⅱ组涂层表面细胞增殖活性具有显著性差异（P＜0.05）;ALP活性测量显示,5d和10d时,2组涂层表面细胞无显著差异（P＞0.05）,随着培养过程的进行,15d和20d时,Ⅰ组涂层表面细胞显著高于Ⅱ组涂层表面细胞（P＜0.05）;蛋白含量检测表明,Ⅰ组涂层表面细胞自5d至20d始终高于Ⅱ组涂层表面细胞（P＜0.05）.结论：HA涂层表面纳米颗粒的直径可以影响成骨细胞的增殖活性和功能代谢,颗粒直径接近体内骨组织HA纳米晶体的涂层,生物相容性较好.     

rhBMP-7对大鼠成骨细胞增殖及分化能力的影响

目的研究重组人骨形态发生蛋白-7（recombinant human bone morphogenetic proteins,rhBMP-7）对大鼠成骨细胞增殖和分化能力的影响。方法从SD大鼠取材进行原代培养,经碱性磷酸酶（alkaline phosphatase,ALP）和矿化结节检测鉴定为成骨细胞后传代至第3代,接种至96孔培养板。分为A、B、C、D、E、F共6组,每组3个复孔,rhBMP-7浓度分别为0．000、0．500、1．000、5．000、10．000、50．000mg/L。应用四甲基偶氮唑盐（methyl thiazolyl tetrazolium,MTT）法及PNPP偶氮法,分析不同浓度rhBMP-7作用72h后对大鼠成骨细胞增殖和ALP活性的影响,应用单因素方差分析和LSD法统计数据。结果作用72h后,6种浓度rhBMP-7对大鼠成骨细胞ALP活性促进作用的差异有统计学意义（F=11．840,P=0．000）,B组成骨细胞ALP活性高于A组,但差异无统计学意义（P=0．078）,C、D、E、F组大鼠成骨细胞ALP活性均高于A组（P值均为0．000）,rhBMP-7浓度为50．000mg／L时对ALP活性的影响最明显（P=0．000）。作用72h后6种浓度rhBMP-7对大鼠成骨细胞增殖影响的差异有统计学意义（F=2．726,P=0．026）;rhBMP-7能促进大鼠成骨细胞的增殖和分化,同A组（rhBMP-7浓度为0．000mg／L）相比,当rhBMP-7浓度为50．000mg／L时并作用至第3天时,大鼠成骨细胞增殖最显著（P=0．000）。结论一定剂量的rhBMP-7明显促进了大鼠成骨细胞的增殖和分化。     

瘦素在种植体骨结合中的作用研究

摘要：目的：体外实验研究瘦素对大鼠骨髓间充质干细胞在纯钛表面增殖、分化的影响。 方法：将体外培养的SD大鼠骨髓间充质干细胞接种于钛片表面,实验组加入50nmol/L瘦素,比较实验组与对照组ALP、OC的含量,以及MTT法检测瘦素对细胞增殖的影响。 结果：实验组与对照组之间ALP、OC情况均有统计学差异（P<0.05）,对细胞增殖无明显促进作用。 结论：瘦素能够促进钛片表面骨髓间充质干细胞的分化。     

nHACP／CF材料与成骨细胞体外复合培养的实验研究

目的:检测甲壳素纤维增强多孔胶原基骨组织工程支架材料(nHACP/CF)对成骨细胞附着增殖的影响,为骨组织工程支架材料的选择提供实验依据.方法:原代培养大鼠成骨细胞,将第3代细胞与nHACP/CF材料体外复合培养.碱性磷酸酶(ALP)染色对成骨细胞进行鉴定;将不同浓度成骨细胞接种到nHACP/CF支架上,通过检测ALP活性测定最佳接种浓度;通过扫描电镜观察和各培养时点细胞增殖率及ALP活性检测,评价nHACP/CF材料对成骨细胞的影响.采用SPSS11.5软件包对测定结果进行单因素方差分析.结果:大鼠成骨细胞ALP染色阳性.统计分析表明,6×105/ml为细胞最佳接种浓度;大鼠成骨细胞能在nHACP/CF材料上附着、增殖,其生长及功能不受抑制.结论:nHACP/CF材料是大鼠成骨细胞附着生长的良好载体.     

盐酸二甲双胍对大鼠下颌骨成骨细胞增殖、分化及矿化功能的影响

目的：研究盐酸二甲双胍（MF）对大鼠下颌骨成骨细胞增殖、分化及矿化功能的影响，为糖尿病患者经种植牙给药假说提供实验依据。方法：分离培养原代下颌骨成骨细胞，分别给予不同浓度的MF，MTT法检测大鼠下颌骨成骨细胞的增殖、生化法测定碱性磷酸酶（ALP）活性、钙吸收、茜素红S钙染法检测矿化结节形成。结果：在一定浓度范围内，MF可以提高成骨细胞数量和ALP活性，促进钙吸收及矿化结节形成。结论：MF能促进原代下颌骨成骨细胞的增殖分化及矿化，提高成骨细胞的成骨能力。     

低温氩氧等离子体处理的无机牛骨对MC3T3-E1细胞黏附、增殖及分化的影响

目的研究小鼠胚胎成骨细胞MC3T3-E1在低温氩氧等离子体处理的无机牛骨上的黏附、增殖及分化特征。方法使用低温氩氧等离子体对无机牛骨进行表面活化(实验组)后,使用扫描电子显微镜(SEM)观察无机牛骨表面形貌的变化,X射线光电子能谱分析(XPS)检测表面元素的组成。将MC3T3-E1细胞接种于低温氩氧等离子体处理的无机牛骨表面,使用SEM观察细胞的黏附形态,CCK-8法检测细胞1、3、5 d的增殖变化,碱性磷酸酶(ALP)法检测细胞7、14 d的分化状态。以不作处理的无机牛骨作为对照。结果对照组与实验组的表面形貌无明显改变;在表面材料的元素组成上,实验组表面碳元素减少,氧、钙、磷元素均增高。实验组MC3T3-E1细胞的黏附更充分,细胞伸出伪足;培养1、3、5 d时,实验组细胞增殖数量明显高于对照组;培养14 d时,实验组ALP活性明显高于对照组(P<0.05)。结论低温氩氧等离子体处理对无机牛骨表面小鼠胚胎成骨细胞的黏附、增殖及分化具有一定的促进作用。     

P-6/ABM复合物促进骨缺损修复的初步实验研究

目的：研究细胞结合肽(P-6肽)与无机骨粒(ABM)复合物在骨缺损修复中的作用。方法：将P-6／ABM复合物植入兔颅骨缺损区，分别于2、4、8周进行X线、组织学观察，以ABM植入组为对照，评价其骨修复能力。结果：结果表明X线、组织学检查P-6／ABM组较ABM组有更多新骨形成。结论：P-6／ABM复合材料具有较强的促进骨缺损修复作用。     

A comparative evaluation of the effectiveness of an anorganic bone matrix/cell binding peptide with an open flap debridement in human infrabony defects: a clinical and radiographic study

AIM: The development of biologic modalities designed to enhance bone regeneration and wound healing of specific periodontal sites continues to unfold. This is accomplished through the cell binding activity of Type-I collagen provided by a synthetic cell binding peptide (P-15) which is incorporated in a scaffold of anorganic bovine matrix (ABM). This combination is designed to facilitate the attachment, migration, and differentiation of cells. The objective of this study is to clinically and radiographically evaluate the effectiveness of the combination of ABM and P-15 (ABM/P-15) 'putty' during regenerative periodontal procedures. METHODS AND MATERIALS: A total of 20 interproximal intraosseous defects in 16 patients, (8 males, 8 females), age 22-48 years (mean 34.45) were recruited and divided equally into two experimental groups. Following open flap debridement (OFD), the defect sites in a test group were grafted with a bovine-derived xenograft enriched with a cell binding peptide. The defect sites in a control group were treated with only OFD. Appropriate periodontal maintenance schedules were followed; at six months, clinical and radiographic assessments for soft tissue and hard tissue were performed for documentation and finalization of results. RESULTS: Statistical analysis using student paired 't' test analyses of the patient mean value from the 16 patients revealed the ABM/P-15 'putty' graft group demonstrated significantly better mean defect fill of 3.4 + 0.7 mm (70.5%) versus mean defect fill of 0.9 mm (17.33%) for defects treated with only OFD. Soft tissue findings showed significant differences among treatment with ABM/P-15 compared to OFD. CONCLUSIONS: These results indicate the use of P-15 synthetic cell binding peptide combined with ABM yields better clinical results in conjunction with OFD than with OFD alone.     

Bone regeneration of localized chronic alveolar defects utilizing cell binding peptide associated with anorganic bovine-derived bone mineral: a clinical and histological study

BACKGROUND: Osteoinduction to treat osseous defects has been attempted by several means. Some clinical studies have demonstrated that a synthetic cell binding peptide (P-15) with anorganic bovine derived bone matrix (ABM) has the ability to enhance bone regeneration. These studies suggest that more histological data are necessary to better understand this process. We have developed a Class III chronic alveolar defect animal model to investigate space-maintaining regenerative materials. The objective of this study was to clinically and histologically evaluate the use of P-15/ABM with or without a bioabsorbable membrane (M) to regenerate localized chronic alveolar ridge defects in dogs. METHODS: Six adult, male mongrel dogs were used in this study. Bilateral, Class III, alveolar defects were surgically produced following extraction of the mandibular second premolar teeth and local reduction of the alveolar ridge. After an 8-week healing interval, mucoperiosteal flaps were elevated. P-15/ABM with or without bioabsorbable membranes were implanted into contralateral defects in 10 sites. Two sites received no biomaterial (controls). Mucoperiosteal flaps were advanced over the P-15/ABM or P-15/ABM/M constructs and sutured. Pre- and postaugmentation clinical evaluation was done utilizing periodontal probes and calipers. The animals were sacrificed 12 weeks postaugmentation and block specimens processed for histologic evaluation. RESULTS: Clinical results showed no significant statistical augmentation on the control group (0.0 +/- 0.6 mm). In all experimental sites utilizing P-15/ABM or P-15/ABM/M, relevant ridge augmentation was observed (3.6 +/- 2.0 mm and 2.9 +/- 1.9 mm, respectively). Histologically, all experimental sites showed active bone formation with plump osteoblast and osteoid matrix deposition in the treated area. Bone ingrowth filled the area of the defects treated with P-15/ABM/M. Few P-15/ABM particles were seen in the cellular fibrous tissue surrounding the new formed bone trabeculae. CONCLUSIONS: P-15/ABM with or without membranes can produce a significant clinical ridge augmentation. Bone formation was histologically observed in all test areas. The association of a membrane with P-15/ABM seemed to enhance the process of bone formation.     

牙囊细胞条件培养液在大鼠脂肪间充质干细胞向成牙骨质分化中的作用

目的：探讨牙囊细胞条件培养液（DFCCM）在诱导大鼠脂肪间充质干细胞（ADSCs）向成牙骨质样细胞分化中的作用。方法：分离培养大鼠脂肪间充质干细胞、牙囊细胞,制备DFCCM。用DFCCM诱导ADSCs,通过MTT检测ADSCs增殖能力的变化;免疫荧光及实时定量PCR方法检测成骨关键蛋白一骨钙素（OCN）、碱性磷酸酶（ALP）及成牙骨质关键蛋白一牙骨质附着蛋白（CAP）,牙骨质蛋白（CP23）的情况。结果：牙囊细胞条件培养液诱导ADSCs后可抑制ADSCs的增殖能力。DFCCM诱导7d后,ADSCs细胞浆内表达OCN及CAP。同时DFC—CM诱导ADSCs后CAP,CP23,ALP及OCNmRNA表达水平显著高于对照组。结论：牙囊细胞条件培养液构建的微环境可使ADSCs向成牙骨质样细胞分化,为于细胞介导的牙骨质再生提供新的思路。     

富血小板纤维蛋白提取液对牙周膜成纤维细胞成骨能力影响的实验研究

目的：探讨富血小板纤维蛋白提取液（Platelet-rich fibrin extract, PRFe）对牙周膜成纤维细胞（Periodon-tal ligament cell PDLC）增殖、成骨分化的影响,以期为富血小板纤维蛋白在临床的应用提供理论基础。方法：实验分为实验组（P组）和对照组（D组）, P组使用含PRFe的α-MEM成骨诱导培养液（10%胎牛血清、青霉素100mg/L、链霉素100mg/L、10-2mol/Lβ-甘油磷酸钠、10-7mol/L地塞米松、5mg/L抗坏血酸）培养细胞, D组则使用不含PRFe的α-MEM成骨诱导培养液。甲基噻唑基四唑（MTT）法测定1d、3d、5d的细胞增殖数;碱性磷酸酶（ALP）活性检测1d、3d、5d的成骨分化情况;荧光实时定量PCR测定Runx2 mRNA和OCN mRNA基因分别在3d、7d的表达。结果： MTT显示：随培养时间延长,细胞数量逐渐增加,在各时间点, P组细胞的吸光度值（1d：0.235±0.012,3d：0.270±0.014,5d：0.686±0.040）均明显高于 D 组（1d：0.201±0.011,3d：0.286±0.020,5d：0.426±0.024）,差异有统计学意义（P＆lt;0.05）。 ALP活性：随着培养时间延长而增加, P组的增加更为显著,在各时间点, P组的吸光度值（1d：0.124±0.018,3d：0.176±0.013,5d：0.361±0.021）均显著大于D组（1d：0.103±0.011,3d：0.123±0.012,5d：0.162±0.014）,差异有统计学意义（P＆lt;0.05）。荧光实时定量PCR：随着培养时间延长,两组细胞的基因表达量逐渐增加,将D组3d时基因表达水平定义为1,进行组内标准化, P组细胞的Runx2和OCN基因表达量（3d时分别为1.751±0.136,1.510±0.129;7d时分别为2.287±0.165,2.103±0.042）显著高于D组（3d时两个基因均为1;7d时分别为：1.367±0.121,1.208±0.051）,差异有统计学意义（P＆lt;0.05）。结论： PRFe能有效地促进牙周膜成纤维细胞的增殖活性及成骨分化。     

A mineralization-associated membrane protein plays a role in the biological functions of the peptide-coated bovine hydroxyapatite

BACKGROUND AND OBJECTIVE: Anorganic bovine mineral coated with a cell-binding peptide (P-15) is superior to anorganic bovine mineral alone in the treatment of periodontal osseous defects. However, the molecular interactions between P-15 and periodontal ligament fibroblasts remain unclear. MATERIAL AND METHODS: We first compared the in vitro osteogenic activities between periodontal ligament fibroblasts cultured with anorganic bovine mineral alone and with the P-15/anorganic bovine mineral combination. We then harvested the periodontal ligament cell lysate, incubated it with various graft materials, and then washed it to remove unbound proteins. The bound proteins were eluted from graft materials and analyzed using electrophoresis, followed by mass spectrometry and then western blotting. Finally, a neutralizing antibody against one bound protein was added to the cell cultures to repeat the osteogenic assays to clarify its role in the in vitro effects of the P-15/anorganic bovine mineral combination. RESULTS: Cells treated with P-15/anorganic bovine mineral were more viable and showed greater osteogenic activities than cells treated with anorganic bovine mineral alone and the no-graft control. Annexin II, a mineralization-associated protein, bound to P-15/anorganic bovine mineral significantly more than to anorganic bovine mineral alone. The addition of neutralizing antibody for annexin II decreased the osteogenic activities of the P-15/anorganic bovine mineral combination. CONCLUSION: Annexin II of periodontal ligament fibroblasts interacted with the peptide of P-15, and was partially responsible for better in vitro osteogenesis in the P-15 graft.     

Use of anorganic bovine-derived hydroxyapatite matrix/cell-binding peptide (P-15) in the treatment of class II furcation defects: a clinical and radiographic study in humans

BACKGROUND: This study compared clinical and radiographic findings for the treatment of Class II furcation defects in human mandibular molars using anorganic bovine-derived hydroxyapatite matrix (ABM)/cell-binding peptide (P-15) or open flap debridement (OFD). METHODS: Twelve subjects showing two comparable Class II furcation defects in their mandibular molars were enrolled. The defects in each subject were assigned randomly to the test (ABM/P-15) or the control (OFD) group. Clinical measurements and standardized radiographs were taken at baseline and 6 to 7 months after surgery. RESULTS: There were no statistically significant differences between the test and control groups for any clinical or radiographic parameter (P >0.05). On comparing the baseline and final measurements, the gain in horizontal clinical attachment level and reduction in gingival recession were significant only in the test group (P < or =0.02), whereas the gain in the vertical clinical attachment level was significant in both groups (P < or =0.04). In the test group, four of 12 sites showed complete closure, and five showed partial closure; in the control group, three defects showed complete closure, and four showed partial closure (P = 0.42). Subtraction radiography revealed similar gains in bone height and increases in mean bone density with both treatments (P >0.05). CONCLUSIONS: ABM/P-15 yielded favorable results in the treatment of Class II furcation defects over a 6-month evaluation period; however, there was no difference compared to OFD. Further studies using a larger sample size are needed to confirm the present findings.     

促矿化液对大鼠成骨细胞增殖分化的影响

目的比较大鼠成骨细胞在矿化条件与非矿化条件下增殖和分化的过程,评价促矿化液对其生理功能的影响,明确促矿化液加入细胞培养环境的适宜时间。方法取SD大鼠头盖骨做成骨细胞原代培养,将增殖稳定后的第4代细胞分别在矿化条件和非矿化条件下培养,检测其形态、碱性磷酸酶活性、细胞周期等。结果大鼠成骨细胞增殖基本稳定后促矿化液组与无促矿化液组细胞分裂增殖指数相似,但前者碱性磷酸酶活性明显较高且持久。结论成骨细胞增殖基本稳定后加入促矿化液对细胞增殖无影响,但可明显促进细胞矿化功能,是加入促矿化液诱导细胞矿化功能的较好时机。     

神经生长因子对骨髓基质细胞成骨活性的影响

目的 探讨神经生长因子（nerve growth factor,NGF）对骨髓基质细胞成骨活性的影响.方法 原代培养SD大鼠四肢骨骨髓源性骨髓基质细胞.通过检测细胞增殖、定量检测碱性磷酸酶的表达及茜素红矿化结节染色,观察NGF对骨髓基质细胞增殖及成骨活性的影响.结果 实验组与对照组细胞增殖无统计学差异,NGF对骨髓基质细胞增殖无促进作用.第3天时,实验组细胞分化及碱性磷酸酶的表达高于对照组（P＜0.05）.钙结节茜素红染色及钙结节量实验组大于对照组.结论 NGF可直接促进骨髓基质细胞的成骨向转化及矿化作用,对细胞增殖无促进作用.     

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目的采用微弧氧化(micro-arc oxidation,MAO)技术,在纯钛表面制备含锌(Zn)活性涂层,并评价其对成骨细胞活性的影响。方法本实验于2009年10月至2010年10月在中国医科大学附属口腔医院中心实验室进行。以机械抛光纯钛试样作为对照组(CP组);经MAO技术处理的纯钛涂层试样根据钙(Ca)含量不同分为高钙组(H-Ca组)、低钙组(L-Ca组),以及在低钙组的涂层中加入锌元素的低钙加锌组(L-Ca-Zn组)。每组32个试件。将4组试样材料与MG63细胞复合培养,利用扫描电子显微镜(SEM)观察、噻唑蓝(MTT)比色试验、碱性磷酸酶(ALP)活性测定及酶联免疫吸附试验(ELISA)等方法,评价材料对成骨细胞黏附、增殖、分化及矿化的影响。所得数据采用SPSS13.0软件进行单因素方差分析(α=0.05)。结果在实验检测期间,H-Ca组试样对成骨细胞的生长增殖、分化、矿化作用均优于L-Ca组,差异有统计学意义(P<0.05)。比较L-Ca-Zn组与H-Ca组可以看出,在实验检测初期H-Ca组试样对成骨细胞的生长增殖、分化、矿化作用优于L-Ca-Zn组;随着观察时间的延长,L-Ca-Zn组试样细胞的增殖水平和ALP活性与H-Ca组间差异无统计学意义(P>0.05)。比较L-Ca-Zn组和L-Ca组可以看出,在实验检测初期L-Ca-Zn组试样对成骨细胞的作用与L-Ca组差异无统计学意义(P>0.05);随着观察时间的延长,L-Ca-Zn组试样显示出较L-Ca组试样具有更好的提高成骨细胞活性的作用(P<0.05)。结论采用MAO技术制备的含锌活性涂层纯钛可在一定程度上促进成骨细胞黏附、增殖、分化及矿化,具有良好的生物相容性。