维生素E抗D-半乳糖致衰老小鼠的脂质过氧化作用

目的 探讨维生素E对D-半乳糖致衰老小鼠抗氧化能力的影响。方法 用D-半乳糖制备衰老小鼠模型。同时维生素E灌胃给予治疗。7周后，用722分光光度计检测衰老小鼠肾、肝、睾丸组织超氧化物歧化酶(superoxide dismutase；SOD)活性、丙二醛(malondialdehyde；MDA)含量。结果 维生素E可显著提高D-半乳糖致衰老小鼠肾、肝、睾丸组织SOD活性，并使衰老小鼠体内MDA含量明显下降。结论 维生素E可显著提高D-半乳糖致衰老小鼠的抗氧化能力。     

衰老大鼠睾丸组织SOD、MDA和血清睾酮含量的变化

目的:观察D-半乳糖致亚急性衰老大鼠和自然衰老大鼠睾丸组织SOD、MDA和血清睾酮含量的变化。方法:D-半乳糖连续腹腔注射建立亚急性衰老大鼠模型,并以自然衰老大鼠和正常大鼠作为对照,分别检测各组大鼠睾丸组织SOD、MDA的含量,放射免疫法检测血清睾酮含量。结果:与正常组大鼠比较,模型组和自然衰老组大鼠血清睾酮含量、睾丸组织SOD含量明显降低,睾丸组织MDA含量明显升高(P0.01);三个指标模型组和自然衰老组比较,差异无统计学意义(P0.05)。结论:衰老大鼠睾丸抗氧化能力明显降低,睾丸间质细胞分泌睾酮的功能发生障碍,且D-半乳糖致衰老模型是自然衰老的很好再现。     

CHANGES OF SOD, MDA CONTENT IN TESTIS AND SERUM TESTOSTERONE CONTENT OF AGING RATS

目的:观察D-半乳糖致亚急性衰老大鼠和自然衰老大鼠睾丸组织SOD、MDA和血清睾酮含量的变化.方法:D-半乳糖连续腹腔注射建立亚急性衰老大鼠模型,并以自然衰老大鼠和正常大鼠作为对照,分别检测各组大鼠睾丸组织SOD、MDA的含量,放射免疫法检测血清睾酮含量.结果:与正常组大鼠比较,模型组和自然衰老组大鼠血清睾酮含量、睾丸组织SOD含量明显降低,睾丸组织MDA含量明显升高(P＜0.01);三个指标模型组和自然衰老组比较,差异无统计学意义(P＞0.05).结论:衰老大鼠睾丸抗氧化能力明显降低,睾丸间质细胞分泌睾酮的功能发生障碍,且D-半乳糖致衰老模型是自然衰老的很好再现.     

脂糖舒对D-半乳糖衰老模型小鼠组织SOD活性和MDA含量的影响

目的观察脂糖舒对D-半乳糖衰老模型小鼠组织超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量的影响。方法采用D-半乳糖制造小鼠衰老模型，同时灌胃不同剂量脂糖舒（Ⅰ组5g／kg、Ⅱ组10g／kg，1次／d），连续6周，并取健康小鼠10只作正常对照。6周末检测小鼠心、肝、肾和脑组织中SOD活性和MDA含量。结果模型组与正常组比较，小鼠各组织中SOD活性明显降低，MDA含量明显升高，P〈0．05或P〈0．01；而脂糖舒两剂量组较模型组小鼠心、肝、肾组织中SOD活性明显升高，MDA含量明显降低，P〈0．05或P〈0．01；脂糖舒两剂量组间的SOD活性和MDA含量无显著性差异（P〉0．05）。结论脂糖舒能通过提高抗氧化能力来发挥延缓衰老的作用。     

二陈汤对亚急性衰老小鼠器官指数及自由基代谢的影响

[目的]探讨二陈汤理气化痰抗衰老的作用机理。[方法]采用D-半乳糖小鼠亚急性衰老模型。小鼠颈背部皮下注射D-半乳糖（120mg/kg/d)40天，中药组注射D-半乳糖同时以6.32g/kg/d浓缩二陈汤煎液灌胃40天，并以空白组作对照，观察二陈汤对小鼠脑，胸腺，脾，肝，肾指数及自由基代谢指标血清超氧化歧化酶（SOD），丙二醛（MDA）的影响。[结果]D-半乳糖诱导的亚急性衰老小鼠脑，胸腺，脾，肝和肾的重量指数明显下降，SOD活性明显下降。MDA含量明显提高，二陈汤能够提高实验动物脑，胸腺指数。提高血清SOD活性，降低MDA含量。[结论]二陈汤具有一定的抗衰老作用。并为“痰老衰相关性学说”理论提供了实验依据。     

蓝莓对D-半乳糖模型小鼠学习记忆及抗氧化的研究

目的:探讨蓝莓对D-半乳糖致衰老小鼠学习记忆能力及抗氧化作用的影响。方法:连续给小鼠注射D-半乳糖6周,同时从第3周起给模型鼠灌胃蓝莓,给药结束后,进行学习记忆行为测定,采血清测定超氧化歧化酶(SOD),丙二醛(MDA)含量。结果:蓝莓组小鼠的学习、记忆获得和记忆消退的错误次数明显少于衰老模型组(P<0.05);与模型组比较,蓝莓组小鼠血清SOD活性显著性降低。结论:蓝莓能改善D-半乳糖致衰老小鼠的学习记忆能力,提高体内SOD活性,增强其对自由基的清除能力,抑制脂质过氧化作用。     

松蘑提取液对D-半乳糖致衰老小鼠功能的影响

目的 观察松蘑提取液对D-半乳糖致衰老小鼠功能的影响.方法 50只小鼠随机分成对照组、模型组和松蘑提取液低、中、高剂量组(100 mg/mL、150 mg/mL、200 mg/mL).采用D-半乳糖复制衰老模型,检测小鼠脏器指数,学习记忆功能,测定大脑皮层和海马组织SOD、GSH-Px活性及MDA含量.结果 模型组小鼠空间学习记忆功能下降,逃避潜伏期延长;皮层和海马组织SOD、GSH-PX活性降低,MDA含量增高.松蘑提取液治疗组能明显改善衰老小鼠的空间学习记忆能力,显著提高皮层和海马中SOD、GSH-PX活性,降低MDA水平,呈剂量依赖性.结论 松蘑提取液减轻了衰老小鼠的学习记忆障碍,其机制可能与其抗氧化作用有关.     

D-半乳糖衰老大鼠抗氧化能力及血脂含量的观测

目的:观察D-半乳糖衰老大鼠抗氧化能力及血脂含量的变化.方法:D-半乳糖连续腹腔注射制作亚急性衰老的大鼠模型,检测大鼠血清中超氧化物歧化酶(SOD)、丙二醛(MDA)、总胆固醇(TC)、甘油三酯(TG)和高密度脂蛋白(HDL-C)的含量.结果:衰老大鼠血清SOD活性下降、MDA水平升高,与正常大鼠相比有显著差异(P＜0.01);衰老大鼠血清中TC、TG的含量明显高于正常大鼠(P＜0.05,P＜0.01);模型大鼠血清中HDL-C的含量则明显低于正常大鼠(P＜0.05).结论:D-半乳糖衰老大鼠抗氧化能力下降、血脂代谢紊乱,这可能是D-半乳糖致衰老的作用机制之一.     

银杏叶滴丸对不同衰老小鼠肝MDA、S0D影响的研究

目的:探讨银杏叶滴丸对自然衰老小鼠和D-半乳糖水衰老模型小鼠MDA含量、SOD活性的影响.方法:设正常对照组、自然衰老对照组及干预组、D-半乳糖模型对照组及干预组,干预组给予银杏叶滴丸,对照组给予蒸馏水.取各组肝组织匀浆,按试剂盒说明测定MDA、SOD活性.结果:各组肝匀浆中MDA均低于其对照组、SOD均高于其对照组(P<0.05).D-半乳糖干预组SOD活性、MDA含量与正常对照组相比差异无显著性(P>0.05),其余各组均低于正常对照组(P<0.05).结论:银杏叶滴丸具有抗氧化作用,进而延缓衰老.     

D-半乳糖老化模型小鼠抗氧化能力的实验研究

目的：研究D-半乳糖脑老化模型小鼠脑组织抗氧化能力的变化。方法：实验动物随机分为正常对照组和衰老模型组，通过给小鼠腹腔注射D-半乳糖建立衰老动物模型。8周后，处死小鼠制备大脑皮层电镜标本和脑组织生化指标的测定。结果：衰老模型组小鼠大脑超氧化物歧化酶（SOD），一氧化氮合酶（NOS）活性下降，丙二醛（MDA）、脂褐素含量升高，一氧化氮（N0）水平下降。结论：D-半乳糖衰老模型小鼠脑组织抗氧化能力下降。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.