常用消毒剂对多重耐药鲍曼不动杆菌的体外抑制作用

目的 明确临床常用消毒剂对我院ICU病房流行的多重耐药鲍曼不动杆菌的最低抑制浓度（MIC）值,帮助临床选取正确的多重耐药鲍曼不动杆菌消毒剂.方法 收集本院ICU流行鲍曼不动杆菌临床分离株8株,制备菌液,以微量稀释法测定临床常用消毒剂对8株鲍曼不动杆菌的MIC值.结果 碘伏、过氧乙酸,氯己定、2%戊二醛和乙醇对8株鲍曼不动杆菌的MIC值分别为0.001 6%、0.01 1%、62.5mg/L、1%和75% 肥皂对鲍曼不动杆菌无作用.5种消毒剂对鲍曼不动杆菌的有效稀释比,从高到低依次是过氧乙酸、氯己定、碘伏、戊二醛和乙醇.结论 过氧乙酸、氯己定为本院抑杀环境中多重耐药鲍曼不动杆菌的首选消毒剂,而2%戊二醛不主张应用.碘伏能够作为体表中的有效消毒剂,而乙醇应用消毒时一定要保证浓度在75%.我们认为消毒剂对于不同细菌的MIC值检测正如抗菌药物敏感试验一样对于控制细菌感染性疾病的流行有着重要价值.     

267株鲍曼不动杆菌感染临床分布与耐药性分析

目的了解鲍曼不动杆菌的临床分布及其对常用抗牛素的耐药状况,为临床使用抗牛素治疗提供依据。方法对我院2009年1月～2009年12月住院患者送检标本分离的287株鲍曼不动杆菌的分布及药敏结果进行回顾性分析。结果267株鲍曼不动杆菌大多分离自痰液,主要来自于ICU病房;鲍曼不动杆菌对头孢西丁耐药率最高,为100％,依次是氨曲南（85．0％）、哌拉西林（76．8％）、哌拉西彬三唑巴坦（71．5％）、替卡西林／克拉维酸（70．8％）、头孢噻肟（67．8％）、头孢毗肟（65．2％）等。结论我院鲍曼不动杆菌耐药情况严重,治疗首选碳青霉烯类抗生素,其次可选用头孢哌酮／舒巴坦。     

60株鲍曼不动杆菌喹诺酮类耐药基因分析

目的探究鲍曼不动杆菌喹诺酮类耐药的机制。方法收集2009年1-12月天津市三所三甲医院各类标本分离的鲍曼不动杆菌60株。采用聚合酶链反应（PCR）扩增技术对质粒介导喹诺酮类耐药基因qnrA、qnrB、qnrS、acc（6’）．Ib．cr、qepA及染色体基因gyrA和parC进行基因检测,并对阳性结果进行酶切和测序鉴定。结果60株鲍曼不动杆菌中qnrA、qnrB、qmS和qepA基因均为阴性,acc（6’）-Ib基因12株阳性,经测序证实均未出现变异。37株环丙沙星耐药的鲍曼不动杆菌中30株（81．0％）gyrA基因不被HinfI酶切,26株（70％）parc基因不被Hinf I酶切,证实有基因突变存在。结论天津地区尚未发现鲍曼不动杆菌中存在质粒介导的喹诺酮耐药机制,gyrA和parC基因变异仍为鲍曼不动杆菌喹诺酮耐药的主要原因,但同时亦有其他喹诺酮耐药机制的存在。     

228例老年鲍曼不动杆菌肺部感染的临床分析

目的了解老年鲍曼不动杆菌肺部感染的临床特点。方法分析228例老年鲍曼不动杆菌肺部感染患者的症状、体征、肺部影像学改变、细菌耐药现状及治疗转归等。结果老年鲍曼不动杆菌肺部感染多于基础疾病的危重期起病。临床症状和体征缺乏特征性。肺部X线影像学变化以小片状浸润影多见（占84．2％）。鲍曼不动杆菌对常用抗生素的耐药率为5．2％-86．3％。患者住院病死率为19．7％。结论If缶床上应重视鲍曼不动杆菌所引起的老年肺部感染。     

泛耐药菌感染老年患者的细菌耐药性分析及其临床意义

目的分析泛耐药菌感染老年患者的细菌耐药性及其临床意义。方法对感染了铜绿假单胞菌和鲍曼不动杆菌的302例老年患者进行回顾性研究,收集病患者临床标本（痰、血、尿、胆汁、伤口等）,进行细菌鉴定和药敏实验;根据实验结果进行分组：泛耐药菌感染组和非泛耐药菌感染组。观察两组患者治愈率和死亡率并行组间比较;对泛耐药菌感染患者的基础疾病和用药情况进行分析。结果302例老年患者中检测出泛耐药菌感染患者34例（泛耐药菌感染组）和非泛耐药菌感染患者268例（非泛耐药菌感染组）。泛耐药菌感染组治愈率明显低于非泛耐药菌感染组（35％vs75．4％,P〈0．01）,死亡率却显著高于非泛耐药菌感染组（18％vs3．7％,P〈0．01）。结论泛耐药菌的耐药性对老年患者危害极大,应该采取有效措施加以解决。     

天津市某三甲医院2011～2013年常见致病菌的耐药率及其变化趋势

目的：了解天津市某三甲医院2011～2013年临床常见致病菌的耐药率及其变化趋势。方法分析天津市某三甲医院2011年1月～2013年12月6种常见病原菌对常用抗生素的耐药资料,并对耐药率变化进行统计学分析。结果金黄色葡萄球菌和表皮葡萄球菌对利奈唑胺均敏感,没有发现对万古霉素耐药的金黄色葡萄球菌,肺炎克雷白菌和大肠埃希菌对哌拉西林他唑巴坦和头孢哌酮舒巴坦的耐药率均较低（＜15％）,没有发现对碳青霉烯类抗生素耐药菌株,鲍曼不动杆菌和铜绿假单胞菌对头孢哌酮舒巴坦的耐药率均较低（＜10％）,但鲍曼不动杆菌对其耐药率上升（P＜0．01）,表皮葡萄球菌和铜绿假单胞菌对部分抗生素的耐药率上升（P均＜0．05）,肺炎克雷白菌、大肠埃希菌、鲍曼不动杆菌对头孢吡肟、头孢曲松、头孢他啶的耐药率均有所下降（P均＜0．05）。结论常见致病菌对部分抗生素耐药率有下降趋势,耐药结果可对临床抗感染经验用药提供参考。     

ICU肺炎患者中泛耐药鲍曼不动杆菌的感染危险因素及耐药性分析

目的：调查ICU肺炎患者中泛耐药鲍曼不动杆菌的感染危险因素,分析其耐药性情况。方法：2013年1月到2016年2月选择入住我院ICU病房后诊断为泛耐药鲍曼不动杆菌肺炎患者98例作为观察组,同期按照2:1的比例选择在我院ICU病房诊治的非泛耐药鲍曼不动杆菌肺炎患者49例作为对照组,两组都采集痰液标本进行耐药性分析,同时调查了泛耐药鲍曼不动杆菌感染的危险因素与预后情况。结果：观察组对于头孢噻肟、哌拉西林、亚胺培南、庆大霉素、左氧氟沙星、四环素、头孢他啶都高度耐药,仅对多粘菌素敏感。除哌拉西林、多粘菌素外,对照组对其他抗菌药物的耐药率均明显低于观察组(P<0.05)。单因素分析显示糖尿病、感染前住院时间、入院24h内APACHEⅡ评分、GCS评分、深静脉置管、机械通气等因素与 ICU 内泛耐药鲍曼不动杆菌性肺炎显著相关(P<0.05)。非条件 Logistic回归分析,结果显示糖尿病、感染前住院时间、入院24h内APACHEⅡ评分、GCS评分导致泛耐药鲍曼不动杆菌感染的独立危险因素(P<0.05)。结论：ICU肺炎患者中泛耐药鲍曼不动杆菌感染比较常见,糖尿病、感染前住院时间、入院24h内APACHEⅡ评分、GCS评分为主要的危险因素,其对于绝大多数抗菌药物有耐药性,可导致患者预后变差与随访死亡率增加。     

肺结核合并鲍曼不动杆菌感染的临床特点及对常用抗生素的耐药性分析

目的 探讨肺结核患者合并鲍曼不动杆菌（Ab）感染的临床特点,以及分析鲍曼不动杆菌对常用抗生素的耐药性.方法 收集2009年1月至2012年12月入住山东省胸科医院的肺结核合并鲍曼不动杆菌感染患者129例,采集合格痰标本分别接种至血琼脂、巧克力琼脂及麦康凯琼脂培养基分离培养,采用VITEK 32全自动微生物分析系统进行药物敏感性试验,将鲍曼不动杆菌对常用抗生素如头孢哌酮舒巴坦和亚胺培南等共计18种药物的敏感性试验数据进行耐药性分析.数据采用世界卫生组织细菌耐药性监测软件WHONET5.6软件进行分析.结果 129例患者中共分离出138株鲍曼不动杆菌,2009年25株（18.1％）,2010年32株（23.2％）,2011年39株（28.3％）,2012年42株（30.4％）.鲍曼不动杆菌对常用抗生素耐药率逐年上升,其中耐药率上升最快的为头孢哌酮舒巴坦和亚胺培南.2009年对上述药物的耐药率分别为4.0％（1/25）、12.0％（3/25）;2010年分别为9.4％（3/32）、18.8％（6/32）; 2011年分别为26.6％（10/39）、28.2％（11/39）;2012年分别为50.0％（21/42）、45.2％（19/42）.结论 与2009年相比,2012年结核病合并鲍曼不动杆菌感染患者比率明显上升,对常用抗生素的耐药率也逐年上升.     

耐亚胺培南鲍曼不动杆菌耐药性及同源性分析

目的明确我院临床分离亚胺培南耐药鲍曼不动杆菌的耐药谱特点及其同源性。方法采用Vitek-32全自动细菌鉴定药敏系统及纸片扩散法（K-B法）测定鲍曼不动杆菌对16种抗菌药物的敏感性;通过重复序列引物聚合酶链反应（rep-PCR）分析其基因同源性。结果33株亚胺培南耐药株除对头孢哌酮-舒巴坦的耐药率〈10％,对其他抗菌药物的耐药率均在54．5％以上;rep-PCR将鲍曼不动杆菌分为E1、E2、E3三个基因型,其中A型15株,为主要的流行型别。结论我院流行的亚胺培南耐药鲍曼不动杆菌的多重耐药现象严重,且主要是由同一克隆株传播所致。     

体外碳青霉烯类抗生素联合舒巴坦对广泛耐药鲍曼不动杆菌抗菌活性研究

目的探讨比阿培南、业胺培南联合舒巴坦对广泛耐药鲍曼不动杆菌体外协同抗菌作用。方法应用棋盘格法测定比阿培南与亚胺培南分别联合舒巴坦对22株广泛耐药鲍曼不动杆菌协同抗菌作用。当比阿培南联合舒巴坦表现出协同作用或拮抗作用时,应用时间杀菌曲线法进一步评价联合用药时抗菌药物相耳：作用。结果应用棋盘格法比阿培南联合舒巴坦对于所测广泛耐药鲍曼不动杆菌表现协同作用的为36．d％（S／22）,部分协同作用的为40．9％（9／22）,相加作用为4．5%（1／22）,无关作州为18．1％（4／22）,无菌株出现拮抗作用;亚胺培南联合舒巴坦分别为31．8％（7／22）协同作用,45．5％（10/22）部分协同作用,4．5％（1／22）相加作用,18．1％（4／22）无关作用,九菌株出现拈抗作用。根据敏感性不同将所测菌株分为对比阿培南或亚胺培南低水平耐药组（8mg／L〈MIC≤32rng／L）和高水平耐约组（MC≥64mg／L）,低水平耐药组联合舒巴坍后对比阿培南（15株）或亚胺培南（16株）MIC90降至8mg／L,达到敏感范围。对于棋盘格法比阿培南联合舒巴坦表现出协同作用的8株广泛耐药鲍曼小动杆菌,应用时间杀菌曲线法重新评估,3株（3／8）表现出阱同作用,末发现拈抗作用。结论比阿培南联合舒巴坦与亚胺培南联合舒巴坦对与广泛耐药鲍曼不动杆菌多表现出协同或部分协同作用。联合舒巴蛆对于比阿培南或亚胺培南低水平耐药鲍曼不动杆菌感染的治疗可能有效。棋盘格法与时间杀菌曲线法所得结果存在一定差异。     

Efficacy of common antiseptics against multidrug-resistant Mycobacterium tuberculosis.

OBJECTIVES: The incidence of infections caused by multidrug-resistant strains of Mycobacterium tuberculosis (MDR-TB) has been increasing. Antiseptics are frequently used to prevent mycobacterial infection. The aim of this study was to determine those antiseptics that are useful against MDR-TB. DESIGN: We evaluated bactericidal activity against clinical isolates of MDR-TB in vitro. METHOD: Thirteen strains of MDR-TB were tested against povidone-iodine (PVP-I), cresol, akyldiaminoethyl glycine hydrocloride (AEG), and glutaraldehyde. After bacilli were exposed to the antiseptic solution with 2% human serum, the disinfectant was inactivated by addition of neutraliser. RESULTS: PVP-1 at a final concentration of 0.2% killed all of the strains within 120 seconds, and PVP-I at 0.1% killed 99.9% or more bacilli within 60 seconds. Most strains were killed after exposure to 0.5% cresol at 300 seconds and to 1.0% cresol at 60 seconds; 3.0% cresol killed all bacilli within 120 seconds, while 0.1%, 0.2%, and 0.5% AEG all required 60 minutes to kill 99.9% or more of the bacilli; 2.0% glutaraldehyde required 10 minutes to kill all bacilli. CONCLUSION: The bactericidal activities of antiseptics for MDR-TB were similar to those for drug-sensitive M. tuberculosis strains. PVP-I would be a useful antiseptic against MDR-TB. The bactericidal activities of glutaraldehyde are effective against MDR-TB as an antiseptic for medical equipment.     

GI flexible endoscope disinfection: "in use" test comparative study

BACKGROUND/AIMS: There is some confusion as to the effectiveness of the available disinfectants for achieving "high level" disinfection, and the microbiologic method to assess the efficacy of the selected disinfectant regime. The "in use" method is adequate for control and for establishing comparisons between different disinfectants. METHODOLOGY: This study compares the efficacy of the different disinfectants and disinfection regimes available, including automatic systems to the 20-minute immersion in 20 degrees C 2%-alkaline-glutaraldehyde (AG). After cleaning and disinfection the effluent obtained from each channel was collected under sterile conditions. A total of 0.1 mL of the effluent was introduced in liquid thioglycolate, an additional 0.1 mL was seeded in solid blood agar and in MacConkey agar medium, and maintained for 48 hours at 37 degrees C. Thioglycolate media turbidity after a 48-hour culture indicates bacterial growth. RESULTS: The disinfectants used were 2% AG, 0.125% and 0.27% glutaraldehyde, glutaraldehydephenol-phenate, peracetic acid, N-duopropenida, 13%-H2O2-27%-lactic acid and ortho-phtalaldehyde using manual and automated methods. Most of the disinfectants available obtain similar or better results compared with 20' 2% AG. The best results (bacterial reduction greater than 3 log10), were those obtained using 20-minute 1/4 or 1/2 glutaraldehydephenol-phenate, 10-minute peracetic acid, or hydrogen-peroxide compounds, 5-minute 0.125% and 0.27% AG at high temperature and 5-minute 0.5% ortho-phtalaldehyde. CONCLUSIONS: A sensitive microbiologic method described may be useful in the control of disinfection and allowed: 1) knowledge of the limits of the efficacy of the disinfection methods usually used, 2) effective comparison of the different disinfectants and disinfection regimes and 3) awareness of the need for microbiologic regulations in assessing "high level" disinfection.     

不同处理条件下脱细胞脐动脉制备小口径组织工程人工血管生物力学性质的对比研究

目的:测定使用3种不同方法处理人脱细胞脐动脉支架后,分别植入种子细胞组装的小口径人工血管生物力学性质,为小口径人工血管的研究提供参考。方法:取同一条人脱细胞脐动脉,均分3段并分三组:A组为0.9%氯化钠溶液处理组,B组为75%乙醇处理组,C组为0.5%戊二醛处理组。然后在特制的搏动培养装置中,将种子细胞的混悬液种植于血管支架内表面;最后对其进行组织学及生物力学性能检测,并相互比较。结果:制备的三组小口径人工血管中B组及C组的塑形性较A组好;力学测试结果显示:抗张力强度C组最大(4.98±0.16)N,B组次之(3.27±0.11)N,A组最小(1.79±0.13)N(B组与A组比较P=0.023;C组与A组比较P=0.027);抗爆破压强度:B组(541±20)mmHg(1mmHg=0.133kPa),C组(605±24)mmHg,两组均大于A组(386±18)mmHg(B组与A组比较P=0.035;C组与A组比较P=0.042);位移-负荷曲线分析显示:,C组的斜率最大,B组次之,A组最小;HE染色结果显示:AB两组血管的内皮细胞数量、覆盖程度均优于C组;扫描电镜的检测结果显示:A组与B组的细胞种植情况也好于C组;结论:使用75%乙醇或0.5%戊二醛处理后的脱细胞脐动脉塑形性好,血管形态结构好、生物力学性质均明显较0.9%氯化钠液处理组好;而75%乙醇处理后的脱细胞脐动脉在其顺应性方面较0.5%戊二醛处理后更接近于生理状态、且种子细胞种植效果相对较好,因此75%乙醇处理的人脱细胞脐动脉是较理想的血管支架材料。     

EFFICACY OF HIGH‐LEVEL DISINFECTANTS FOR GASTROINTESTINAL ENDOSCOPE DISINFECTION AGAINST STRONGYLOIDES STERCORALIS

Background: Guidelines for high‐level disinfection of gastrointestinal endoscopes have been published in many countries; however, none of them have a statement for disinfection against the parasite Strongyloides stercoralis. To establish a method for gastrointestinal endoscope disinfection against Strongyloides stercoralis, we examined the disinfection efficacy of high‐level disinfectants (3% glutaraldehyde, 0.55% ortho‐phthalaldehyde, and 0.3% peracetic acid) and 70% ethanol by in vitro tests. Methods: Strongyloides stercoralis larval suspension (0.05 mL) was added to 0.95 mL of each disinfectant and disinfection time measured under low magnification microscopy. Results: Among the high‐level disinfectants, peracetic acid was more efficacious for disinfection against Strongyloides stercoralis than glutaraldehyde and ortho‐phthalaldehyde. Ethanol was also efficacious for parasite killing. Conclusion: These results suggested that peracetic acid be recommended for gastrointestinal endoscope disinfection in regions with endemic Strongyloides stercoralis.     

Efficacy of common antiseptics against mycobacteria.

SETTING AND OBJECTIVE: Antiseptics are frequently used to prevent mycobacterial infection; however, the reported activities of a number of antiseptics against mycobacteria are not always consistent. The aim of this study was to determine those antiseptics that are useful against mycobacteria. DESIGN: Evaluation of antiseptic activity against mycobacteria in vitro. RESULTS: The effects of different antiseptics on mycobacteria (Mycobacterium avium, M. kansasii and M. tuberculosis) were examined. At concentrations of 0.05%, povidone-iodine (PVP-I) killed 99% or more of all strains tested within 15 seconds, while 0.5% chlorhexidine gluconate and 0.1% benzalkonium chloride showed no bactericidal activity against mycobacteria. M. kansasii and M. tuberculosis were killed after exposure to cresol for 60 seconds at concentrations of 1.0%, but M. avium was unaffected even after 60 seconds. While M. kansasii and M. tuberculosis were killed by treatment with 2.0% glutaraldehyde for 5 minutes, M. avium was highly resistant to this agent. CONCLUSION: PVP-I seems to be a useful antiseptic against mycobacteria. The measured activity of antiseptics should be interpreted carefully, due to the potential for interference by artifacts.     

Surrogate Selection for Foot-and-Mouth Disease Virus in Disinfectant Efficacy Tests by Simultaneous Comparison of Bacteriophage MS2 and Bovine Enterovirus Type 1

In South Korea, testing disinfectants against foot-and-mouth disease virus (FMDV) that are contagious in livestock or that require special attention with respect to public hygiene can be manipulated only in high-level containment laboratories, which are not easily available. This causes difficulties in the approval procedure for disinfectants, such as a prolonged testing period. Additionally, the required biosafety level (BSL) in the case of FMDV has hindered its extensive studies. However, this drawback can be circumvented by using a surrogate virus to improve the performance of the efficacy testing procedure for disinfectants. Therefore, we studied bacteriophage MS2 (MS2) and bovine enterovirus type 1 (ECBO) with respect to disinfectant susceptibility for selecting a surrogate for FMDV according to the Animal and Plant Quarantine Agency (APQA) guidelines for efficacy testing of veterinary disinfectants. Effective concentrations of the active substances in disinfectants (potassium peroxymonosulfate, sodium dichloroisocyanurate, malic acid, citric acid, glutaraldehyde, and benzalkonium chloride) against FMDV, MS2, and ECBO were compared and, efficacies of eight APQA-listed commercial disinfectants used against FMDV were examined. The infectivity of FMDV and ECBO were confirmed by examination of cytopathic effects, and MS2 by plaque assay. The results reveal that the disinfectants are effective against MS2 and ECBO at higher concentrations than in FMDV, confirming their applicability as potential surrogates for FMDV in efficacy testing of veterinary disinfectants.     

Inactivation of Cryptosporidium parvum oocyst infectivity by disinfection and sterilization processes

BACKGROUND: Cryptosporidium parvum is a common cause of self-limited gastroenteritis in the normal host but may cause severe disease in immunocompromised persons. Person-to-person transmission has been well documented in households, child care centers, and hospitals. Because contaminated environmental surfaces and medical devices such as endoscopes may play a role in disease transmission, we studied the susceptibility of C parvum to chemical agents commonly used for disinfection and evaluated the efficacy of sterilization processes. METHODS: Seven disinfectants were studied at their use dilution using a suspension test. Antimicrobial activity was assessed with the use of a cell infectivity assay. RESULTS: All sterilization processes tested (steam, ethylene oxide, Sterrad 100) inactivated 3 logs or greater of C parvum. The only liquid disinfectant/sterilant able to inactivate greater than 3 logs of C parvum was 6% and 7.5% hydrogen peroxide. Agents that did not completely inactivate C parvum included hydrogen peroxide at lower concentrations or exposure times, peracetic acid, sodium hypochlorite, a phenolic, a quaternary ammonium compound, 2% glutaraldehyde, and ortho-phthalaldehyde. CONCLUSIONS: Most high-level disinfectants used on endoscopes have limited efficacy against C parvum. However, the infectivity of C parvum on dry surfaces decreases rapidly. Therefore, current cleaning and high-level disinfection guidelines are adequate to prevent nosocomial transmission of C parvum by means of endoscopes.     

我院45株金黄色葡萄球菌临床分布及消毒剂耐药基因qacA的检测

目的 分析我院耐甲氧西林金黄色葡萄球菌（MRSA）临床分布及耐消毒剂基因qacA检测情况，为合理使用消毒剂、减少院内感染提供依据。方法收集2007年1月-2008年6月分离自河北医科大学第一医院住院患者的blRSA45株，采用PCR方法对其耐消毒剂基因qacA进行检测。结果45株MRSA中18株（40％）检出qacA基因，其中呼吸内科、烧伤科标本检出率较高，分别为13％、16％。结论我院呼吸内科和烧伤科等科室MRSA分离率及qacA基因阳性率较高，此为常用消毒剂耐药的主要机制；临床应根据患者标本qacA检测情况合理选用消毒剂。     

Efficacy of various disinfectants in killing a resistant strain of Pseudomonas Aeruginosa by comparing zones of inhibition: implications for endoscopic equipment reprocessing

Objective: Previous studies have shown that high-level disinfection of GI endoscopes may not be reliably achieved using glutaraldehyde at room temperature. In our laboratory, we have isolated a strain of Pseudomonas aeruginosa that is resistant to disinfection with glutaraldehyde. We compared the bactericidal activity of various disinfectants against this organism.
Methods: One hundred microliters of an overnight culture of this organism was spread onto blood agar plates. Twenty microliters of a disinfectant was placed on a sterile 7-mm filter paper, placed on the blood agar plate, and incubated overnight at 37°C to determine the zone of inhibition for each disinfectant tested. Disinfectants included Cidex, Dispatch, Virahol, OMNI II, Lysol, IodoFive, Lysol I.C. Spray, and Chlorox. The zone of inhibition ( i.e ., clearing) roughly correlates with the bactericidal strength of the disinfectant.
Results: Compared with the glutaraldehyde-containing solution Cidex, the alcohol-containing disinfectants Lysol I.C. Spray and Virahol had the largest mean zones of inhibition (11.33 vs 20.60 and 20.55 mm;   p = 0.0001  ). The hypochlorite compounds Chlorox (1:10 dilution) and Dispatch had mean zones of inhibition similar to that of Cidex (11.08 and 11.25 mm vs 11.33 mm;   p = not significant  ). The phenolic compounds OMNI II and Lysol had mean zones of inhibition smaller than that of Cidex (10.50 and 10.35 mm vs 11.33 mm;   p < 0.006  ), and the phosphoric acid and iodine–containing IodoFive had the smallest mean zone of inhibition (9.70 vs 11.33 mm;   p = 0.0001  ).
Conclusions: The alcohol-containing disinfectants had the largest zones of inhibition against resistant P. aeruginosa . These compounds may be more effective than glutaraldehyde for endoscopic equipment reprocessing.