FOXP3、GITR和GITRL在口腔扁平苔藓患者外周血中的表达

目的:研究FOXP3和GITR/GITRL基因在口腔扁平苔藓(OLP)患者外周血中的表达水平。方法:采用实时荧光定量RT-PCR的方法,检测30例OLP患者和15例健康人外周血中FOXP3、GITR和GITRL的基因表达水平。结果:OLP组FOXP3、GITR和GITRL mRNA表达量均显著低于健康对照组(P<0.01);结果经2-ΔΔCT转换后,OLP组FOXP3、GITR和GITRL mRNA表达水平分别是健康对照组的23.4%、18.1%和20.9%;FOXP3和GITR的表达水平呈正相关关系(r=0.491,P<0.05)。结论:FOXP3和GITR/GITRL mRNA的表达异常,影响了调节性T细胞(Treg)的功能。     

IP-10和IL-6在口腔扁平苔藓中表达的研究

目的:检测正常口腔黏膜与口腔扁平苔藓(OLP)黏膜的上皮和固有层结缔组织中干扰素诱导蛋白10(IP-10)、细胞因子白细胞介素-6(IL-6)mRNA的表达,探讨其在OLP中的作用。方法:应用荧光实时定量RT-PCR技术检测10例OLP黏膜和6例正常口腔黏膜的上皮和固有层结缔组织中IP-10、IL-6mRNA的表达量。结果:OLP黏膜上皮和固有层结缔组织中IP-10、IL-6mRNA的表达均较正常口腔黏膜明显升高(P<0.05),但IP-10、IL-6mRNA的表达量没有明显差异(P>0.05)。结论:OLP组织中趋化因子IP-10、细胞因子IL-6表达量改变可能与其发生发展有一定关系。     

PD-L1、PD-L2 mRNA在OLP组织中的表达研究

目的:研究PD-L1和PD-L2 mRNA在OLP损害组织中的表达.方法:采用Real-time PCR方法,检测PD-L1和PD-L2 mRNA在20例OLP不伴异常增生、10例OLP伴轻度异常增生损害组织中的表达水平,并以10例健康人正常黏膜为对照.结果:PD-L1 mRNA在不伴异常增生组、伴轻度异常增生组的表达水平分别较正常对照组上调1.3倍、1.7倍,但3组之间均无显著性差异(p＞0.05).PD-L2 mRNA在不伴异常增生组的表达水平较正常对照组上调3.0倍,两者之间有显著性差异(p＜0.01);OLP伴轻度异常增生组的表达水平较正常对照组上调3.2倍,两者之间有显著性差异(p＜0.01);OLP不伴异常增生组与伴轻度异常增生组之间无显著性差异(p＞0.05).结论:PD-L2可能在OLP的发病机制中起重要作用,而PD-L1可能并未参与OLP的发病.     

Ets-1在口腔扁平苔藓中的表达研究

目的 :分析Ets 1(E2 6transformation specific)在口腔扁平苔藓 (OLP)的表达及意义。 方法 :采用免疫组化ABC法检测Ets 1蛋白在 2 0例口腔扁平苔藓和 8例正常口腔黏膜组织中的表达。结果 :70 % (14 /2 0 )的口腔扁平苔藓病例中Ets 1呈阳性表达 ,明显高于正常黏膜组织 ,两者比较有显著性差异 (P <0 .0 5 )。阳性表达率在溃疡型OLP与斑块型OLP间亦有显著性差异 (P <0 .0 5 ) ,并与病程相关 (P <0 .0 5 )。结论 :Ets 1在口腔扁平苔藓中过表达并与其发病有关。     

口腔扁平苔藓差异表达MicroRNAs的筛选

目的:芯片筛选口腔扁平苔藓(oral lichen planus,OLP)损害黏膜中差异表达的miRNAs。方法:收集白纹型OLP损害黏膜和正常口腔黏膜各2例,抽提总RNA预扩增后运用Taqman低密度芯片(v2.0)测定人类667种miRNAs的表达谱,随后应用实时定量PCR在68例样本中进一步验证miR-27b的表达水平。采用miRNAs生物信息库预测miR-27b所对应的靶基因,对芯片数据进行统计学分析。结果:筛选获得30个OLP相关的miRNAs。相对于正常口腔黏膜,OLP损害黏膜中有18个miRNAs表达下调,12个上调。扩大样本量至68例验证miR-27b表达水平,结果与芯片实验一致。生物信息学分析得出miR-27b的预测靶基因中有10个与OLP发病有关。结论:筛选得到的OLP损害黏膜中差异表达的miRNAs可能与OLP的发生相关。     

HERG1在口腔扁平苔藓和口腔鳞状细胞癌中的表达

目的：研究钾离子通道蛋白HERG1在正常口腔黏膜（normal oral mucosa,NOM）、口腔扁平苔藓（oral lichen planus,OLP）、口腔鳞状细胞癌（squamous cell carcinoma,OSCC）中的表达及意义。方法：免疫组织化学技术检测16例NOM、20例OLP、30例OSCC组织中HERG1的表达。采用χ2检验,P〈0.05为差异具有统计学意义。结果：HERG1在OSCC中的表达强度高于OLP（P〈0.05）,HERG1在OLP组织的表达高于正常组织（P〈0.05）,且糜烂型OLP中的表达高于非糜烂型OLP（P〈0.05）,差异均具有统计学意义。结论：HERG1可能与OLP及OSCC的发生发展有一定的关系。     

膜联蛋白A1表达下降在口腔鳞癌中的意义

目的：研究膜联蛋白A1（ANXA1）在口腔鳞癌中的表达及其与临床病理的关系。方法：应用实时定量PCR、Western印迹方法和免疫组化方法检测ANXA1在口腔鳞癌细胞株和30例原发口腔鳞癌中的表达，采用SPSS10．0软件包对数据进行非参数检验。结果：与人永生化口腔黏膜上皮细胞（HIOEC）相比，Tca8113、TSCC、OSC和NT细胞中ANXA1的mRNA表达降低；Tca8113、TSCC、CAL-27、OSC和NT细胞中ANXA1的蛋白表达降低。30例口腔鳞癌组织标本中，ANXA1的mRNA和蛋白表达均较癌旁组织降低（p〈0．001）。ANXA1的表达水平与肿瘤的病理分化程度有关（mRNA：P=0．007；蛋白：P=0．006），与肿瘤大小、临床分期、淋巴结转移无关。结论：ANXA1在口腔鳞癌中表达降低，可能与肿瘤的发生、发展和组织分化有关。     

STIM1在口腔扁平苔藓患者中表达的研究

目的：通过观察基质相互作用分子1（stromal interaction molecule 1,STIM1）在口腔扁平苔藓（oral lichen planus,OLP）患者外周血淋巴细胞中的表达,探索 OLP的发病机制。方法：采用实时定量 RT-PCR的方法,检测37例 OLP 患者及17例健康成人外周血淋巴细胞中的 STIM1基因表达水平;采用 Western blot法检测上述对象外周血淋巴细胞中的 STIM1蛋白表达水平。结果：糜烂型和非糜烂型 OLP患者中 STIM1基因和蛋白的表达水平均高于对照组,且差异有统计学意义（P＜0．01）;但糜烂型和非糜烂型 OLP患者组间表达的差异均无统计学意义（P＞0．05）。结论：STIM1在 OLP 外周血淋巴细胞中高表达,通过对淋巴细胞的免疫调控参与了 OLP的发病过程。     

转化生长因子β刺激基因22在口腔扁平苔藓组织中的表达及意义

目的 探讨转化生长因子β刺激基因22(TGF beta-stimulated clone 22,TSC22)在口腔扁平苔藓(OLP)分子病理机制中的作用方法采用实时荧光定量PCR方法,检测24例口腔扁平苔藓组织,12例正常口腔黏膜组织中TSC22mRNA的表达水平。结果 TSC22mRNA在口腔扁平苔藓病变组织中的表达水平低于正常口腔黏膜组织(P<0.001)。结论 TSC22的异常表达在OLP的发病机制中起到重要作用。     

少突胶质细胞谱系基因-髓鞘碱性蛋白在口腔扁平苔藓组织中的表达

目的：探讨少突胶质细胞谱系基因一髓鞘碱性蛋白（genes of the oligodendrocyte lineage—myelin basic protein,Golli—MBP）在口腔扁平苔藓（orallichenplanus,OLP）组织中的表达及其与OLP发病的关系。方法：采用实时荧光定量PCR的方法,检测39例OLP患者病损组织和16例健康口腔黏膜组织中Golli—MBP的基因表达水平,采用免疫组化法检测38例OLP及20例正常口腔黏膜组织石蜡标本中Golli—MBP的表达情况。结果：Golli一删即mRNA在OLP组中的表达显著高于正常对照组（P〈0．001）。OLP患者石蜡标本中Golli—MBP的表达水平亦显著高于对照组（P〈0．001）;固有层淋巴细胞中Golli—MBP的表达高于对照组（P〈0．05）;上皮细胞中Golli—MBP的表达在OLP患者及对照组间无统计学差异（P〉0．05）。结论：Golli—MBP在OLP组织中高表达,可能在OLP的发病机制中起一定作用。     

KLF-6在口腔扁平苔藓恶变中的表达研究

目的 探讨KLF 6在口腔扁平苔藓发病机制及癌变中的作用。方法 分别采用免疫组化和Western blot方法对10例正常口腔黏膜、30例扁平苔藓患者和22例口腔鳞癌患者的上皮组织中KLF 6蛋白进行t检测,比较其在三者之间的差异。SPSS 14.0对数据进行检验。结果 扁平苔藓患者组织中KLF 6蛋白的表达明显低于正常口腔黏膜,两组之间具有显著性差异;口腔鳞癌患者组织中KLF 6蛋白的表达明显低于扁平苔藓组织,两组之间具有显著性差异。结论 KLF 6的表达异常可能在口腔扁平苔藓的发生发展及癌变的过程中起作用。     

A clinical study of 674 patients with oral lichen planus in China

BACKGROUND: Oral lichen planus (OLP) is a chronic inflammatory disease with different clinical presentations that can be classified as reticular, atrophic or erosive. Although OLP is a relatively common disorder, the reports comprising large numbers of OLP patients with specific character are lacking in the literature. The purpose of this paper was to describe the clinical characteristics of OLP in 674 Chinese patients. METHODS: A total of 674 charts of patients with histologically confirmed OLP were collected from Stomatological Hospital of Wuhan University between 1963 and 2003. RESULTS: Of the 674 patients, 65.9% were women and 34.1% were men. The most common clinical presentation was the reticular type (51.3%), and symptomatic OLP was noted in 67.5% of the patients, mainly in those with the erosive form. The erosive presentations showed significantly longer duration, more sites affected and a much greater old patients predominance than reticular or atrophic ones. About 90.9% of the patients had multiple oral sites of involvement and isolated lower lip lichen planus were observed in 60 cases (8.9%) and isolated gingiva lichen in only one case (0.2%). Skin involvement of lichen planus was found in 11.4% of patients. No statistically significant differences could be identified between OLP and diabetes, cardiovascular disease, smoking or alcohol use. Precipitating factors that resulted in an exacerbation of the disease were frequently noted and included foods, stress, dental cusp and poor oral hygiene. The transformation of OLP into malignancy was observed in four patients at sites previously diagnosed by clinical examination as erosive or atrophic lichen planus. CONCLUSIONS: Patients with OLP in China usually present with distinctive clinical morphology and characteristic distribution and few may display lesions with a confusing array of forms mimicking other diseases. A long time follow up is of utmost importance to detect its malignant transformation.     

Th1 cytokines in oral lichen planus

BACKGROUND: Cell-mediated immune responses in oral lichen planus (OLP) may be regulated by cytokines and their receptors. METHODS: In situ cytokine expression and in vitro cytokine secretion in OLP were determined by immunohistochemistry and ELISA. RESULTS: The majority of subepithelial and intraepithelial mononuclear cells in OLP were CD8+. In some cases, intraepithelial CD8+ cells were adjacent to degenerating keratinocytes. CD4+ cells were observed mainly in the deep lamina propria with occasional CD4+ cells close to basal keratinocytes. Mononuclear cells expressed IFN-gamma in the superficial lamina propria and TNF-alpha adjacent to basal keratinocytes. Basal keratinocytes expressed TNF-alpha as a continuous band. TNF R1 was expressed by mononuclear cells and basal and suprabasal keratinocytes. There was variable expression of TGF-beta1 in the subepithelial infiltrate while all intraepithelial mononuclear cells were TGF-beta1-. Keratinocytes in OLP stained weakly for TGF-beta1. Unstimulated OLP lesional T cells secreted IFN-gamma in vitro. TNF-alpha stimulation down-regulated IFN-gamma secretion and up-regulated TNF-alpha secretion. IL-4, IL-10 and TGF-beta1 secretion were not detected. CONCLUSIONS: These data suggest the development of a T helper 1 immune response that may promote CD8+ cytotoxic T-cell activity in OLP.     

ALDH1在OLP和LK组织中的表达及其意义

目的：通过检测干细胞标记物ALDH1在口腔扁平苔藓（OLP）和白斑（LK）中的表达水平,评价其与癌变的关系。方法：采用免疫组织化学SP法检测ALDH1在10例正常口腔黏膜,30例OLP,60例LK,10例口腔鳞癌（OS－CC）中表达水平;再检测ALDH1在30例癌变与30例未癌变LK中的表达差异。结果：ALDH1在正常口腔黏膜中不表达,在OLP、LK和OSCC中的表达率分别为26．7％,63．3％和90．0％（P＜0．05）;ALDH1在未癌变和癌变LK中的表达率分别是43．3％和83．3％（P＜0．01）。结论：ALDH1与口腔黏膜恶性潜能程度相关,可能是预测癌变的分子标记物。     

Expression of lymphocyte function-associated antigen 3 in oral lichen planus

OBJECTIVE: The expression pattern of lymphocyte function-associated antigen 3 (LFA-3) in the buccal mucosa of oral lichen planus (OLP) patients was compared to that of healthy controls to investigate the possible role of LFA-3 in cell interactions within OLP lesions.
MATERIALS AND METHODS: Samples of buccal mucosa from 17 clinically healthy individuals and 17 OLP lesions were analysed. Expression of LFA-3, CD2, CD3 and CD 14 was visualized by an immunoperoxidase technique and assessed microscopically.
RESULTS: In healthy buccal mucosa LFA-3 was expressed on keratinocytes, Langerhans cells within the epithelium and on endothelial cells in the lamina propria. In OLP patients a similar pattern of LFA-3 staining was observed. In addition, cytoplasmic LFA-3, without accompanying surface staining, was seen on a subpopul-ation of macrophage-like cells. Substantial amounts of LFA-3 also appeared to be associated with non-cellular components of the extracellular matrix within the inflammatory infiltrate.
CONCLUSIONS: We have obtained evidence for a previously undescribed localization of LFA-3 within macro-phages, and have observed that expression of LFA-3 is apparently elevated within OLP lesions. LFA-3 may play an important role in the pathogenesis of OLP.     

A retrospective evaluation of 193 patients with oral lichen planus

One hundred and ninety-three patients with oral lichen planus were evaluated retrospectively. Women outnumbered men by more than two to one. The mean age at lesion discovery was in the sixth decade. The buccal mucosa was the most common site of occurrence. There were concomitant systemic diseases in many of the patients. The ulcerative form was the most prevalent referred form. Therapies included observation and treatment with topical and systemic corticosteroids with and without antifungals and immunosuppressants. Areas of discussion include lesion location, chronicity, therapeutic modalities, malignant transformation, age, gender, symptomatology and systemic disease.