共查询到20条相似文献,搜索用时 15 毫秒
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Brian Grundy Ursula Panzner Jie Liu Hyon Jin Jeon Justin Im Vera von Kalckreuth Frank Konings Gi Deok Pak Ligia Maria Cruz Espinoza Abdramane Soura Bassiahi Nagla Gasmelseed Raphaël Rakotozandrindrainy Suzanne Stroup Eric R. Houpt Florian Marks 《The American journal of tropical medicine and hygiene》2022,106(2):454
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Background and Objectives: We tested dried blood from an ABO bedside test card which had been stored at room temperature for 12 years, to prove that a patient with HIV-1 infection had been infected by blood transfusion. Materials and Methods: Immunoblots for HIV-1 antibodies and threefold PCRs with halfnested primers for the HIV-1 integrase gene were done with eluates from the dried blood spots. Results: HIV-1 antibodies and HIV-1 DNA could be detected in the sample from one unit of blood, but not from the two other units or from the recipient before transfusion. Conclusion: Further studies should be done on the validity of stored dried blood as an alternative to the storage of frozen donor serum for several years for ‘look-back’ studies. 相似文献
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Aakriti Gupta Umesh Kapil Lakshmy Ramakrishnan Preetika Khenduja Chandra Prakash Yadav Nighat Yaseen Sofi Ritika Khandelwal 《Indian journal of hematology & blood transfusion》2017,33(4):565-567
The ability to use indirect cyanmethemoglobin method using 20 µl of dried blood spots (DBSs) on filter paper for the analysis of haemoglobin (Hb) levels could be an important diagnostic tool for areas that have limited access to laboratory facilities. We assessed the validity of a method for Hb estimation in which a single drop of whole blood was directly taken on the filter paper. We collected 124 DBSs containing 20 µl of blood (filter paper A) and single drop of whole blood (filter paper B) from subjects living in Nainital, Uttrakhand. Estimation of Hb was done by indirect cyanmethemoglobin method in both the filter papers. A correction factor for predicting value of Hb from DBSs of single drop of whole blood was established. The Bland–Altman plot suggested that the difference in Hb values obtained by the single drop of blood and 20 µl of DBSs was within the 1.5 SD limits, suggesting high validity of the correction factor. The estimation of Hb using single drop of whole blood on filter paper after applying the correction factor provides results similar to indirect cyanmethemoglobin method using 20 µl of blood. Hence, single drop of whole blood on filter paper can be used as an alternate method for estimation of Hb in large scale community surveys. 相似文献
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verb Asma Aziz K. Zaman Cathinka Halle Julin Firdausi Qadri Kathrine Stene-Johansen Rajib Biswas Shaumik Islam Taufiqur Rahman Bhuiyan Warda Haque Synne Sandbu Jennifer L Dembinski Susanne Dudman 《Viruses》2022,14(11)
Hepatitis E virus (HEV) is the most common cause of acute viral hepatitis worldwide. In many low-income countries it causes large outbreaks and disproportionally affects pregnant women and their offspring. Surveillance studies to find effective preventive interventions are needed but are hampered by the lack of funding and infrastructure. Dried blood spots (DBS) offer an easier and more robust way to collect, transport, and store blood samples compared to plasma/serum samples, and could ease some of the barriers for such studies. In this study we optimize an HEV IgG ELISA for DBS samples and validate it on 300 paired DBS and plasma samples collected in rural areas of Bangladesh from participants in a HEV vaccine study. We demonstrate that HEV IgG in blood stored as DBS is stable for two months at up to 40 °C, and for five freeze-thaw cycles. The specificity was 97% and the overall sensitivity of the DBS assay was 81%. The sensitivity was higher in samples from vaccinated participants (100%) compared to previously infected participants (59%), reflecting a positive correlation between IgG titer and sensitivity. We found a strong correlation between DBS and plasma samples with an r2 of 0.90, but with a higher degree of difference between individual paired samples. Our study shows that DBS offers a stable alternative to plasma/serum for HEV IgG measurements and can facilitate serological studies, particularly in resource limited areas. 相似文献
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《Hemoglobin》2013,37(5):495-504
Hb Chicago is a newly discovered hemoglobin variant which was present in a Black newborn baby and her father. The leucine residue at α136, which normally participates in the contact with the heme group, is replaced by a methionine residue. The two heterozygotes were clinically well with normal hematological data. Isolation of the αx and αA chains by reverse phase high performance liquid chromatography and hydrolysis of these chains with dilute formic acid at 110°C for 24 hours, followed by separation of the resulting peptides by reverse phase high performance liquid chromatography, greatly facilitated the final identification of the abnormality. The baby and both parents had a-Gγ-Gγ-globin gene arrangement on one chromosome (normal:-Gγ-Aγ which explains the high a Gγ values in the Hb F of these three persons. 相似文献
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Bruce D. Gaynor Abdou Amza Boubacar Kadri Baido Nassirou Ousmane Lawan Laouali Maman Nicole E. Stoller Sun N. Yu Stephanie A. Chin Sheila K. West Robin L. Bailey Philip J. Rosenthal Jeremy D. Keenan Travis C. Porco Thomas M. Lietman 《The American journal of tropical medicine and hygiene》2014,90(5):846-851
We assessed the effect of mass azithromycin treatment on malaria parasitemia in a trachoma trial in Niger. Twenty-four study communities received treatment during the wet, high-transmission season. Twelve of the 24 communities were randomized to receive an additional treatment during the dry, low-transmission season. Outcome measurements were conducted at the community-level in children < 1–72 months of age in May–June 2011. Parasitemia was higher in the 12 once-treated communities (29.8%, 95% confidence interval [CI] = 21.5–40.0%) than in the 12 twice-treated communities (19.5%, 95% CI = 13.0–26.5%, P = 0.03). Parasite density was higher in once-treated communities (354 parasites/μL, 95% CI = 117–528 parasites/μL) than in twice-treated communities (74 parasites/μL, 95% CI = 41–202 parasites/μL, P = 0.03). Mass distribution of azithromycin reduced malaria parasitemia 4–5 months after the intervention. The results suggest that drugs with antimalaria activity can have long-lasting impacts on malaria during periods of low transmission. 相似文献
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Joshua Yukich Valerie D'Acremont Judith Kahama Ndeniria Swai Christian Lengeler 《The American journal of tropical medicine and hygiene》2010,83(1):61-68
Rapid diagnostic tests (RDTs) for malaria may help rationalize antimalarial drug use. However, the economic effects of these tests may vary. Data on costs were collected from 259 patients in 6 health facilities by using exit and in-charge interviews and record reviews during a trial of RDT rollout in Dar es Salaam, Tanzania. The RDTs decreased patient expenditure on drugs (savings = U.S. $0.36; P = 0.002) and provider drug costs (savings = U.S. $0.43; P = 0.034) compared with control facilities. However, RDT introduction did not significantly reduce patients'' overall expenditures (U.S. $1.02, 95% confidence interval [CI] = $0.76–$1.36 versus U.S. $1.33 95% CI = $0.99–$1.77) and may increase total provider costs (U.S. $3.63, 95% CI = $3.40–$3.89 versus U.S. $2.32, 95% CI = $1.99–$2.69) compared with control facilities. Clinician''s compliance with test results was higher with RDTs than with routine microscopy (95% versus 82%; P = 0.002). The RDTs reduced drug costs in this setting but did not offset the cost of the tests, although they also resulted in non-monetary benefits, including improved management of patients and increased compliance with test results. 相似文献
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Paul Dantonio Nancy Meredith-Molloy William A. Hagopian Jin Xiong She Beena Akolkar Suzanne K. Cordovado Miyono Hendrix L. Omar Henderson W. Harry Hannon Robert F. Vogt 《Journal of diabetes science and technology》2010,4(4):929-941