多聚酶链反应对病毒性前列腺炎的检测研究

为探讨病毒性前列腺炎的病原学，采用多聚酶链反应（ＰＣＲ）技术对７４例慢性非细菌性前列腺炎的前列腺液进行单纯疱疹病毒Ⅱ型（ＨＳＶⅡ）检测。结果：前列腺液中ＨＳＶⅡ阳性率为１６．２％，对照组均为阴性（Ｐ＜０．０１）。４例前列腺液ＨＳＶⅡ阳性者，前列腺按摩前初始尿和尿道粘膜脱落细胞ＰＣＲ检测均为阴性。结果进一步证实了病毒是非细菌性前列腺炎的重要病因之一，ＰＣＲ技术对病毒性前列腺炎的临床诊断、治疗及疗效判定有重要意义。     

多聚酶链反应对慢性非细菌性前列腺炎的病原学诊断研究

目的 探讨慢性非细菌性前列腺炎的病原学。方法 应用多聚酶链反应（ＰＣＲ）对７６例慢性非细菌性前列腺炎的前列腺液作为单纯疱疹病毒（ＨＳＶ－Ⅱ），沙眼衣原体（ＣＴ），解脲支原体（ＵＵ）检测，同时设对照检测。结果 三种病原体总阳性率为７１％，对照组为６．７％（Ｐ〈０．００５）。两种及两种以上病原体阳性的混合感染率为１５．８％，对照组则为阴性（Ｐ〈０．０１）。结论 ＨＳＶ－Ⅱ，ＣＴ，ＵＵ均为慢性非细菌性前     

PCR检测非细菌性前列腺炎沙眼衣原体的初步研究

对３０例非细菌性前列腺炎（ＮＢＰ）患者的前列腺按摩液（ＥＰＳ）沙眼衣原体（ＣＴ）进行了聚合酶链反应（ＰＣＲ）检测，并与经二乙氨基葡聚糖处理的ＨｅＬａ细菌培养法进行对比研究，结果发现，６例ＰＣＲ和细胞培养双阳性，２１例ＰＣＲ和细胞培养双阴性，３例两种检测结果不一致，包括２例ＰＣＲ阳性但培养阴性，１例培养阳性但ＰＣＲ阴性，将ＰＣＲ与细胞培养进行比较，ＰＣＲ的敏感性为８５．７％，特异性９１．３％，阳性预     

解脲支原体和沙眼衣原体感染与慢性前列腺炎

解脲支原体和沙眼衣原体感染与慢性前列腺炎蒋云仙万声贵王新满毕克菊刘守炎自１９８９年１１月～１９９４年１２月对７８５例慢性前列腺炎患者前列腺液进行了细菌普通培养，解脲支原体（ＵＵ）培养和衣原体（ＣＴ）免疫荧光检测，提示ＵＵ与ＣＴ为慢性非细菌性前列腺炎的...     

尿道粘膜印片检测沙眼衣原体

对１０９例前列腺炎病人尿道粘膜上皮印片进行检测，发现上皮细胞内沙眼衣原体包涵体２１例，革兰阴性双球菌１２例，衣原体包涵体和革兰阴性双球菌同时被检出者１５例，其余６１例二者均无。另外对３２例患前列腺炎、尿道炎及附睾炎的病人，分别作前列腺液涂片和尿道粘膜印片检查，结果发现，印片的阳性检出率明显高于前列腺液涂片的阳性率。提示临床医师应注意被检标本的采集。     

尿道粘膜印片检测沙眼衣原体

对１０９例前列腺炎病人尿道粘膜上皮印片进行检测，发现上皮细胞内沙眼衣原体包涵体２１例，革兰阴性双球菌１２例，衣原体包涵体和革兰阴性双球菌同时被检出者１５例，其余６１例二者均无。另外对３２例患前列腺炎、尿道炎及附睾炎的病人，分别作前列腺液涂片和尿道粘膜印片检查，结果发现，印片的阳性检出率明显高于前列腺液涂片的阳性率，提示临床医师应注意被检标本的采集。     

慢性非细菌性前列腺炎患者前列腺液革兰阴性菌16SrRNA基因的检测

目的　探讨慢性非细菌性前列腺炎的可能病因。　方法　应用PCR方法检测 2 4例慢性非细菌性前列腺炎患者和 10例正常人前列腺液中革兰阴性菌 16SrRNA基因。同时对 10例实验组患者行尿道分泌物PCR检查。　结果　 2 4例慢性非细菌性前列腺炎患者前列腺液中革兰阴性菌16SrRNA基因阳性 13例 ,阳性率 5 4 % ;10例正常人中阳性 1例 ,阳性率 10 % (P <0 .0 1)。 10例慢性非细菌性前列腺炎尿道分泌物PCR均阴性。　结论　慢性非细菌性前列腺炎患者前列腺液革兰阴性菌 16SrRNA基因阳性率显著高于正常人。革兰阴性菌 16SrRNA基因源于前列腺而非尿道。一些目前还不能常规成功培养的细菌可能为慢性非细菌性前列腺炎的发病原因之一。     

慢性前列腺炎病原学的初步研究

目的对慢性前列腺炎的尸体前列腺组织标本进行病原学、病理组织学变化的研究,并对病原体与病理组织学变化的相关性进行初步探讨。方法从１９９６年５月～１９９７年１２月,对１１４例２０～４０岁慢性前列腺炎组织标本用细菌学培养、多聚酶链反应（ＰＣＲ）,对组织内的沙眼衣原体、解脲脲原体、病毒、淋球菌进行检测及病理组织学检查。结果１１４例前列腺组织标本中病原体检测阳性５９例（５２％）,其中单纯细菌性病原体阳性４２例,单纯沙眼衣原体（ＣＴ）６例,人类巨细胞病毒（ＣＭＶ）２例,解脲脲原体（ＵＵ）１例,人体乳头状病毒（ＨＰＶ）１例,７例为复合病原体。所有标本均存在不同程度的灶性慢性前列腺炎病理组织学变化。临床检测的病原体并不引起特异性病理组织学变化。结论慢性前列腺炎有单纯的病原体感染,还有复合感染,应予重视。慢性前列腺炎发病机制多为逆行感染,其病理组织学变化可能与检测到的病原体无关     

PCR在检测沙眼衣原体前列腺炎中的应用

ＰＣＲ在检测沙眼衣原体前列腺炎中的应用汪定海，赵美芳，单小云自１９９４年１０月～１９９５年１月，对门诊８０例前列腺炎患者采用聚合酶链反应（ＰＣＲ）检测沙眼衣原体。患者年龄１９～５５岁，平均３４．６岁，其中３５例有婚外性接触史，临床症状以尿急尿频，头晕...     

性病后慢性前列腺炎（附65例报告）

为研究性病后慢性前列腺炎病因学特点,对６５例性病后前列腺炎病人进行病原体检测,并通过尿流率测定和性生活状况调查,针对给予治疗。前列腺液细菌培养阳性率为３８．５％（２５６５）。其中以葡萄球菌为主要致病菌（占６０％,１５２５）。前列腺液霉菌检查３例阳性。前列腺液ＰＣＲ检测衣原体阳性１３例,支原体阳性９例,淋球菌阳性１２例。尿流率检测发现８例病人最大尿流率偏低（９．３～１４．６ｍｌｓ）。对１４例病原体检查阴性患者进行性生活状况调查,发现９例患者放弃性生活或性生活频度减少（≤１次月）。认为前列腺液病原体检查,对指导性病后慢性前列腺炎的治疗具有重要临床意义,同时还应注意患者尿流动力学改变和性病后的心理治疗。     

性传播性尿道炎后慢性前列腺炎(附86例报告)

目的　探讨性传播性尿道炎后慢性前列腺炎患者的病因学特点。　方法　对 86例性传播性尿道炎后慢性前列腺炎患者进行前列腺按摩液 (EPS)的病原体检测并分析结果。　结果　EPS细菌培养阳性 5 7例 ,阳性率 6 6 .3% ,其中以淋球菌为主要致病菌 33例 (38.4 % )。前列腺液PCR检测淋球菌阳性 37例 (43.0 % ) ,沙眼衣原体阳性 2 2例 (2 5 .6 % ) ,解脲脲原体阳性 19例(2 2 .0 % )。PCR检测出复合病原体者 12例 (13.9% )。　结论　性传播性尿道炎后慢性前列腺炎的致病菌以淋球菌、衣原体和解脲脲原体为主 ,治疗前行EPS病原学检测有重要意义     

Chlamydia trachomatis survival in the presence of two fluoroquinolones (lomefloxacin versus levofloxacin) in patients with chronic prostatitis syndrome

Fluoroquinolones are recommended in the therapy of chronic prostatitis. Chlamydia trachomatis is one of the possible aetiological agents of chronic prostatitis. However, little is known about chlamydia survival in the presence of fluoroquinolones in patients with chronic prostatitis syndrome. For the first time, chlamydia survival in vitro in the presence of lomefloxacin (LOMX) (mostly recommended in the treatment of chronic prostatitis) versus levofloxacin (LVFX) (recommended in the therapy against chlamydia infection) is examined and analysed in the 33 chlamydia-infected patients with chronic prostatitis syndrome in this study. Antichlamydial activity in vitro of LOMX in patients with C. trachomatis and prostatitis was found to be more effective than LVFX. However, further clinical trials for these agents are recommended.     

Chlamydial and ureaplasmal infections in patients with nonbacterial chronic prostatitis

Our study was designed to establish the necessity of routine evaluation of patients with inflammatory (IIIA) and noninflammatory (IIIB) types of nonbacterial prostatitis (NBP) for chlamydial and ureaplasmal infections. From 1999 to 2001, 165 patients with a mean age of 35 years (range 20-54 years) were evaluated for the syndrome of chronic prostatitis. The evaluation included scoring with Prostate Symptom Score Index (PSSI) and NIH Chronic Prostatitis Symptom Index (CPSI), Meares-Stamey test and culturing of post-massage urine portion (fourth glass). In all cases, polymerase chain reaction (PCR)-testing of the semen was performed to establish the persistence of Chlamydia trachomatis (ChT) and Ureaplasma urealyticum (UU). Based on laboratory findings (four glass test and post-massage urine culture), in 69 (42%) of 165 cases, NBP was diagnosed, which includes 30 patients with type IIIA and 39 with type IIIB of NBP. According to semen PCR tests, in 11 (36.6%) of 30 cases with IIIA type of NBP, chlamydial (six cases), ureaplasmal (four cases) and a mixture of both (one case) infections were described. Among 39 patients with IIIB type of NBP test was positive in 14 cases (36%), where UU was presented in eight and ChT in six cases. In patients with previously diagnosed inflammatory as also noninflammatory NBP, according to four glass test, chlamydial and/or ureaplasmal infections can be presented. Although their role in pathogenesis of prostatitis remains speculative, however, testing for infections is highly recommended.     

Detection of Chlamydia trachomatis in urethra of patients with urogenital infection

Between January, 1984 and August 1986, we examined 151 male patients with urogenital infections including 114 cases of urethritis, 18 cases of epididymitis, 19 cases of prostatitis at the outpatient clinic of the department of urology, Toho University's Ohmori Hospital. The positive rate for Chlamydia trachomatis from the urethra based on cell cultures was 29.0%, (30.5% for urethritis, 27.8% for epididymitis, and 21.1% for prostatitis). The positive rate for non-gonorrheal urethritis was 33.0% and chlamydia infections were complicated by gonorrheal urethritis in 23.3% of the cases. The mean age of the patients with positive Chlamydia trachomatis was 31.9 years. The route of infection in 77.8% of the cases was sexual intercourse the most common cause of which was infection from a prostitute in 62.1% of the cases. The latent period was in most cases from 2-3 weeks.     

慢性前列腺炎病原学病例对照研究

探讨慢性前列腺炎（ＣＰ）的病原学机理，对５３例ＣＰ（病例组）和５７例无泌尿生殖系感染的男性（对照组）进行病例对照研究。比较了两组前列腺液解脲支原体、沙眼衣原体、四杯定位细菌培养、前列腺按压液（ＬＰＳ）白细胞计数和前列腺Ｂ超的结果。病例组ＥＰＳ白细胞〉１０００／ｍｍ＾３的例数高于对照组；病例组金葡菌和大肠杆菌检出率高于对照组，但类白喉杆菌、表皮葡萄球菌和解脲支原体却低于对照组。两组沙眼衣原体检出率无     

Genital infections in men associated withChlamydia trachomatis

The advent of new diagnostic procedures has made it possible for urologists to detect chlamydial presence in genital infections. Many studies have shown chlamydial association with urethritis. However, its presence in prostatitis and epididymitis has not been clearly defined yet. In this study we investigated the presence of Chlamydia trachomatis using enzyme linked immuno assay (ELISA) antigen and antibody detection method in sexually active men with different genital infections. We have found that in 32.1% of patients with non-gonococcal urethritis chlamydial antigen is detectable. We also have been able to detect chlamydial antigen in two patients with chronic non-bacterial prostatitis, but in no patients with epididymitis. We conclude that besides its presence in urethritis, there is an association between chlamydia and chronic prostatitis. Yet, further studies are needed to delineate this association more clearly.     

Significance and problems of Chlamydia detection in urologic patients

Chlamydiae represent the aetiological agent in non-gonococcal urethritis in about 50% of the cases, but it is also increasingly recognized that Chlamydia trachomatis can cause prostatitis or epididymitis. The introduction of methods for the direct detection of chlamydia in clinical materials allows early diagnosis and therefore effective antibiotic therapy. The new tests however give both false positive and false negative results.