SIAH2和HIF-1α蛋白在喉鳞状细胞癌中的表达及临床意义

目的:通过检测喉鳞状细胞癌组织、声带息肉、癌旁组织中SIAH2和HIF-1α蛋白的表达,为喉鳞状细胞癌发病机制提供理论依据,并为其早期诊断和治疗提供帮助. 方法:收集喉鳞状细胞癌患者的石蜡包埋组织60例,同时收集声带息肉、癌旁石蜡包埋组织各30例,应用法免疫组织化学技术检测SIAH2和HIF-1α蛋白的表达水平. 结果:SIAH2蛋白和HIF-1α蛋白在癌旁组织、声带息肉以及喉鳞状细胞癌中的阳性表达率分别为10%、33.3%、70%和3.3%、30.0%和66.7%,差异有统计学意义( P<0.05);SIAH2蛋白和HIF-1α蛋白的表达与喉鳞状细胞癌患者的分化程度、有无淋巴结转移以及临床分期有关( P<0.05). 喉鳞状细胞癌中SIAH2和HIF-1α蛋白的表达呈正相关. 结论:SI-AH2和HIF-1α蛋白可能与喉鳞状细胞癌的发病有关,并对喉鳞状细胞癌的侵袭性生物学行为有促进作用,联合检测二者可为预测肿瘤的生物学行为以及患者的预后提供帮助.     

MDM2蛋白在喉鳞状细胞癌中的表达意义

目的　初步探讨 MDM2在喉癌发生发展中的机制 .方法　采用链霉菌抗生物素蛋白 -过氧化物酶 (S- P)免疫组织化学法 ,检测 1 2例声带息肉 ,1 5例喉癌旁组织 ,1 1例喉不典型性增生及 41例喉鳞状细胞癌组织中 MDM2蛋白的表达 ,并研究蛋白表达与肿瘤病理分级、临床分期、临床分型、淋巴结转移的关系 .MDM2蛋白阳性表达为核着色 .结果　 MDM2蛋白表达阳性率在各个组分别为声带息肉 0 .0 % ,喉癌旁组织40 .0 % ,喉不典型性增生 72 .7% ,喉鳞状细胞癌组织 39.0 % .MDM2蛋白表达在声带息肉组与癌旁组织组、声带息肉组与喉不典型性增生组、声带息肉组与喉鳞状细胞癌组织组、喉不典型性增生组与喉鳞状细胞癌组织组 (P<0 .0 5)均有显著性差异 .喉鳞状细胞癌组 MDM2蛋白表达与临床分期有关 ,与病理分级、临床分型、淋巴结转移无关 .结论　 MDM2基因在喉癌发生、发展中有重要作用 ,其异常表达是喉癌发生中早期分子事件 ,检测 MDM2蛋白有助于喉癌早期诊断     

喉癌中FAK和VEGF的表达及其相互关系的研究

目的:检测粘着斑激酶(Focal adhesion kinase,FAK)和血管内皮生长因子(Vascular endothelial growth factor,VEGF)在喉鳞状细胞癌中的表达及其与侵袭和转移的关系,探讨二者的相关性.方法:采用免疫组织化学S-P法,观察42例喉鳞状细胞癌及40例声带息肉中FAK和VEGF的表达情况.结果:FAK和VEGF在喉鳞状细胞癌中的阳性率分别为79％(33/42)和71％(30/42),在声带息肉中的阳性表达率分别为13％(5/40)和18％(7/40).喉鳞状细胞癌中FAK的表达与病理学分级及肿瘤的原发部位比较差异无统计学意义(P>O.05),与临床分期及颈部淋巴结转移比较差异有统计学意义(P＜0.05);喉鳞状细胞癌中VEGF的表达与临床分期、颈部淋巴结转移比较差异有统计学意义(P＜0.05);FAK与VEGF阳性表达呈正相关(P相似文献   

P120-catenin、VEGF蛋白在喉鳞状细胞癌中的表达及生物学特征研究

目的观察P120-catenin、VEGF蛋白在喉鳞状细胞癌组织中的表达及其与临床病理特征的关系,并探讨它们在喉鳞状细胞癌发生、发展中的作用及临床应用价值。方法应用微波-EliVisionTM免疫组织化学检测P120-catenin蛋白、VEGF蛋白分别在80例喉鳞状细胞癌、30例癌旁组织中的表达。结果①P120-catenin、VEGF蛋白与TNM分期、T分期、淋巴结转移、远处转移有关(P均<0.05),P120-catenin蛋白和VEGF蛋白与分化程度有关(P均<0.05)。②P120-catenin蛋白和VEGF蛋白在喉鳞状细胞癌组织中的表达呈正相关(P<0.01)。结论①P120-catenin和VEGF的高表达对喉鳞状细胞癌发生、发展可能起协同作用。②联合检测P120-catenin和VEGF对预测喉鳞状细胞癌浸润转移有重要意义,并可作为判断喉鳞状细胞癌生物学行为的指标。     

喉鳞状细胞癌中RAGE与amphoterin的表达

目的:探讨RAGE(receptor for advanced glycation end products)与amphoterin在喉鳞状细胞癌中的表达与临床意义。方法:采用免疫组织化学SP法测定RAGE及amphotein在27例喉鳞状细胞癌中的表达,并以声带息肉作为对照组,结合喉鳞状细胞癌的临床特征进行分析。RAGE与amphoterin主要定位于细胞膜上,表达强度按阳性细胞数计算。结果:RAGE在喉鳞状细胞癌的表达明显高于在声带息肉中的表达,转移性喉鳞状细胞癌表达高于非转移性喉鳞状细胞癌。Amphoterin在几乎所有的喉鳞癌中表达,且高于声带息肉中的表达。结论:RAGE与amphoterin的表达与喉鳞状细胞癌的侵袭和转移有关,可作为预测喉鳞状细胞癌转移潜能的指标。     

STK15在乳癌组织中的表达

目的 探讨乳癌组织中癌基因STK15的表达情况.方法 应用免疫组化SP法检测41例乳癌及30例乳腺纤维瘤组织中STK15蛋白的表达.结果 STK15蛋白在乳癌和乳腺纤维瘤组织中的阳性表达率分别为68.3％(28/41)、36.7％(11/30),二者之间差异有显著性(x2=6.999,P＜0.05).STK15蛋白表达与乳癌的TNM分期及组织学分级有关(x2=8.794、8.261,P＜0.05),且STK15蛋白阳性表达强度与乳癌病人的TNM分期有关(x2=5.895,P＜0.05).结论 乳癌组织中存在STK15基因高表达,STK15基因可能是导致乳癌发生的因素之一.     

人喉鳞状细胞癌组织中c-myc和cyclinD1蛋白的表达

目的：检测人喉鳞状细胞癌组织中c-myc和cyclinD1蛋白的表达。方法：采用免疫组化法检测80例喉鳞状细胞癌和30例声带息肉组织中c-myc和cyclinD1蛋白的表达,分析两蛋白的表达与喉鳞状细胞癌患者临床病理特征的关系及二者表达的关联性。结果：喉鳞状细胞癌组织中c-myc和cyclinD1蛋白的阳性表达率分别为...     

ABAD/HADH Ⅱ在喉鳞状细胞癌与声带息肉组织中的表达

目的:检测喉癌组织中ABAD/HADHⅡ的表达,探讨ABAD/HADHⅡ在喉鳞状细胞癌增殖中的作用。方法:选用福建医科大学附属第一医院2002~2005年的27例喉癌手术标本及12例声带息肉手术标本,采用免疫组化检测ABAD/HADHⅡ的表达及分布情况。结果:ABAD/HADHⅡ主要表达在鳞状细胞癌的细胞浆,在生长活跃的细胞表达较高;ABAD/HADHⅡ在喉鳞状细胞癌组织中的表达比在息肉组织中的表达高(P<0.05)。结论:ABAD/HADHⅡ在喉癌组织中表达较正常组织高,与喉鳞状细胞癌的增殖有一定的关系。     

COX-2和Ki-67在喉鳞状细胞癌中的表达及临床意义

目的 探讨喉鳞癌组织中环氧合酶-2(COX-2)和Ki-67表达的相互关系及临床意义.方法 应用免疫组化SP法检测60例喉鳞癌组织、20例声带息肉组织中COX-2、Ki-67蛋白的表达.结果 COX-2蛋白在喉鳞癌和声带息肉组织中的表达差异有统计学意义(P<0.05);COX-2蛋白在喉鳞癌和声带息肉组织中的表达差异也有统计学意义(P<0.05);COX-2和Ki-67蛋白在喉鳞癌组织中的表达分别与分化程度、临床分期和淋巴结转移有关(P均<0.05),而与患者的性别及年龄无关(P均＞0.05).COX-2和Ki-67蛋白在喉癌组织中的表达呈正相关(r=-0.673,P<0.05).结论 COX-2和Ki-67蛋白在喉鳞癌组织中的高表达,可能与喉鳞癌的生长及转移密切相关,同时可作为喉鳞状细胞癌早期诊断及预后判断的分子指标之一.     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.