Hormonal regulation of potassium shifts during graded exhausting exercise

Summary Serum potassium, aldosterone and insulin, and plasma adrenaline, noradrenaline and cyclic adenosine 3:5-monophosphate (cAMP) concentrations were measured during graded exhausting exercise and during the following 30 min recovery period in six untrained young men. During exercise there was an increase in concentration of serum potassium (4.74 mmol·1^–1, SEM 0.12 at the end of exercise vs 3.80 mmol·1^–1, SEM 0.05 basal,P<0.001), plasma adrenaline (2.14 nmol·1^–1, SEM 0.05 at the end of exercise vs 0.30 nmol·1^–1, SEM 0.02 basal,P<0.001), plasma noradrenaline (1.10 nmol·1^–1, SEM 0.64 at the end of exercise vs 1.50 nmol·1^–1, SEM 0.05 basal,P< 0.001), serum aldosterone (0.92 nmol·1^–1, SEM 0.14 at the end of exercise vs 0.36 nmol·1^–1, SEM 0.05 basal,P<0.01), and plasma cAMP (35.4 nmol·1^–1, SEM 2.3 at the end of exercise vs 21.4 nmol·1^–1, SEM 4.5 basal,P<0.05). While concentrations of serum potassium, plasma adrenaline and cAMP returned to their basal levels immediately after exercise, those of plasma noradrenaline and serum aldosterone remained elevated 30 min later (1.90 nmol·1^–1, SEM 0.01,P<0.01; and 0.85 nmol·1^–1, SEM 0.12,P<0.01, respectively). Serum insulin concentration did not change during exercise (6.47 mlU·1^–1, SEM 0.58 at the end of exercise vs 5.47 mlU·1^–1, SEM 0.41 basal, NS) but increased significantly (P<0.02) at the end of the recovery period (7.12 mlU·1^–1, SEM 0.65). Serum potassium increases with exhausting exercise appeared to be caused not only by its release from contracting muscles but also by an -adrenergic stimulation produced by adrenaline and noradrenaline. On the other hand, the increased levels of plasma noradrenaline maintained during the recovery period may have served to avoid excessive hypokalaemia through the stimulation of muscle -receptors. Thus, catecholamines may play an important role in the regulation of serum potassium concentrations during and after exercise. Any disturbance of these adrenergic effects may lead either to an excessive increase or to a decrease of kalaemia, with the consequent risk of arrhythmias linked to exercise.     

Heart rate dynamics after controlled training followed by a home-based exercise program

Daily aerobic training results in autonomic control of the heart toward vagal dominance. The constancy of vagal dominance after controlled training followed by a home-based training program in accordance with contemporary guidelines is not known. We set out here to study whether the vagal dominance induced by 8 weeks of controlled aerobic training is preserved after a 10-month home-based training program. For the controlled study, healthy men were randomized as training (n=18) and control subjects (n=6). The training was started by a supervised 8-week period with six training sessions a week [45 (15) min each] at an intensity of 70–80% of maximum heart rate, followed by a home-based training program for 10 months in accordance with the American College of Sports Medicine recommendations. Cardiovascular autonomic function was assessed by analyzing HR variability over a 24-h period and separately during the night hours (midnight–6 a.m.). Maximal running performance improved during the controlled training 16 (7)% (range 4–31%, P<0.001) and remained 8 (8)% (range –3 to 23%, P<0.001) above the baseline level after the home-based training program. At night, the vagally mediated high-frequency (HF) power of R-R intervals increased during the controlled training from 6.7 (1.3) to 7.3 (1.1) ln ms² (P<0.001) and remained higher than the baseline after the home-based training [7.0 (1.3) ln ms², P<0.05]. The changes in running performance correlated with the changes in HF power at night (r=0.41, P<0.05) and over 24 h (r=0.44, P<0.05) after the home-based training program. Similarly, the changes in body mass index correlated with the changes in HF power over 24 h (r=–0.44, P<0.05) after the home-based training program. The high vagal outflow to the heart after the home-based training is associated with good physical performance and body mass control.     

Strength training effects on physical performance and serum hormones in young soccer players

To determine the effects of simultaneous explosive strength and soccer training in young men, 8 experimental (S) and 11 control (C) players, aged 17.2 (0.6) years, were tested before and after an 11-week training period with respect to the load-vertical jumping curve [loads of 0–70 kg (counter-movement jump CMJ0–70)], 5- and 15-m sprint performances, submaximal running endurance and basal serum concentrations of testosterone, free testosterone and cortisol. In the S group, the 11-week training resulted in significant increases in the low-force portion of the load-vertical jumping curve (5–14% in CMJ0–30, P<0.01) and in resting serum total testosterone concentrations (7.5%, P<0.05), whereas no changes were observed in sprint running performance, blood lactate during submaximal running, resting serum cortisol and resting serum free testosterone concentrations. In the C group, no changes were observed during the experimental period. In the S group, the changes in CMJ0 correlated (P<0.05–0.01) with the changes in the 5-m (r=0.86) and 15-m (r=0.92) sprints, whereas the changes in CMJ40 correlated negatively with the changes in the testosterone:cortisol ratio (r=–0.84, –0.92, respectively, P<0.05). These data indicate that young trained soccer players with low initial strength levels can increase explosive strength by adding low-frequency, low-intensity explosive-type strength training. The inverse correlations observed between changes in CMJ40 and changes in the testosterone:cortisol ratio suggest that a transient drop in this ratio below 45% cannot always be interpreted as a sign of overstrain or neuroendocrine dysfunction.An erratum to this article can be found at     

A systems model of training responses and its relationship to hormonal responses in elite weight-lifters

Summary A systems model, providing an estimation of fatigue and fitness levels was applied to a 1-year training period of six elite weight-lifters. The model parameters were individually determined by fitting the predicted performance (calculated as the difference between fitness and fatigue) to the actual one. The purpose of this study was to validate the systems model by comparing the estimated levels of fatigue and fitness with biological parameters external to the model calculation. The predicted and the actual performances were significantly correlated in each subject. The calculated fitness and fatigue levels were related to serum testosterone concentration, testosterone: cortisol and testosterone: sex hormone binding globulin ratios. The best results were obtained by the comparison between fitness and testosterone levels, which varied in parallel in each subject. In two subjects this correlation was significant (r=0.91, P<0.05, and r=0.92, P<0.01). The fitness changes calculated in each subject between the 15th and the 51st weeks of training were significantly correlated with the changes in serum testosterone concentration measured in the same period (r=0.99, P<0.001). For the whole group testosterone and fitness variations were also significantly intercorrelated (r=0.73, P<0.001). Correlations, less homogeneous and less significant, were calculated also for other hormones and ratios. These results suggest that (1) the relationships between training and performance can be described by the systems model, (2) the estimated index of fitness has a physiological meaning. The fatigue index remains to be clarified.     

Seasonal changes in performance and free testosterone: cortisol ratio of elite female rowers

Summary The aim of this study was to investigate the seasonal behaviour of the plasma free testosterone: cortisol ratio (FTCR) and to relate hormonal changes to daily training volume and performance parameters on a rowing ergometer in elite female rowers. During 9 months of training preceding the 1988 Olympic Games the resting values of the FTCR in six elite female rowers were regularly (ten times) studied. Daily training volume was analysed in terms of rowed distance (l _rowad) and time (t). In addition, two performance parameters, the power at 4.0 mmol·l^–1 lactate concentration in the blood and the maximal power, were determined by a test on a rowing ergometer. The results indicated that the mean FTCR test value did not differ significantly from the level of the initial test or from the mean value of the directly preceding test. A significant negative correlation (r=–0.98, P<0.01) between FTCR and l _rowed was found in a period i.e. at a training camp, when there was a sudden increase in training volume. When FTCR was related to t a significant positive correlation (r=0.88, P<0.05) was found only for the period at the training camp. Our data further suggested that the FTCR alone was not an adequate indicator for the anabolic/catabolic balance in elite female rowers. This finding was contrary to previous findings in elite male rowers. However, in training practice the FTCR seems useful as an indicator of the hormonal training status of elite female rowers when complemented with data about total and free testosterone, performance parameters and knowledge concerning cyclic variations of the FTCR.Both departments co-operate in the working group Janus Jongbloed Research Centre, Utrecht, The Netherlands     

Sustained noradrenaline sulphate response in long-distance runners and untrained subjects up to 2 h after exhausting exercise

Summary We investigated the response of plasma and platelet free catecholamine ([CA]) and sulphated catecholamine ([CA-S]) concentrations after an incremental treadmill test to exhaustion and during recovery. In triathletes (n = 9) plasma and platelet [CA] and [CA-S] were measured before, immediately after and 0.5 and 24 h after exercise. In long-distance runners (n = 9) and in controls (n = 10) plasma [CA] and [CA-S] were determined 2 h instead of 24 h after exercise. Platelet [CA] and [CA-S] remained unchanged throughout the study. Plasma [CA] increased after exercise in all groups (P<0.05) and returned to pre-exercise values within 0.5 h of recovery. Plasma sulphoconjugated noradrenaline concentration ([NA-S]) was elevated after exercise in the triathletes, long-distance runners and in controls [9.96 (SEM 0.84) nmol·1^–1, 11.8 (SEM 1.19) nmol·1^–1, 9.53 (SEM 1.10) nmol·l^–1, respectively;P<0.05] compared with resting values [7.13 (SEM 1.04) nmol·l^–1, 6.19 (SEM 0.56) nmol·l^–1, 6.76 (SEM 0.67) nmol·1^–1, respectively] and remained elevated after 0.5 h of recovery [9.94 (SEM1.14) nmol·l^–1, 10.96 (SEM 0.80) nmol·l^–1, 8.95 (SEM 0.99) nmol·l^–1, respectively;P<0.05]. In the long-distance runners and controls plasma [NA-S] remained elevated during 2 h of recovery [9.96 (SEM 0.76) nmol·l^–1, 9.03 (SEM 0.88) nmol·l^–1, respectively]. These results would indicate that plasma [NA-S] increases after sympathetic nervous system activation by an exhausting incremental exercise test and remain elevated up to 2 h after exercise.     

Blood lactate production and recovery from anaerobic exercise in trained and untrained boys

Summary Blood lactate production and recovery from anaerobic exercise were investigated in 19 trained (AG) and 6 untrained (CG) prepubescent boys. The exercises comprised 3 maximal test performances; 2 bicycle ergometer tests of different durations (15 s and 60 s), and running on a treadmill for 23.20±2.61 min to measure maximal oxygen uptake. Blood samples were taken from the fingertip to determine lactate concentrations and from the antecubital vein to determine serum testosterone. Muscle biopsies were obtained from vastus lateralis. Recovery was passive (seated) following the 60 s test but that following the treadmill run was initially active (10 min), and then passive. Peak blood lactate was highest following the 60 s test (AG, 13.1±2.6 mmol·l^–1 and CG, 12.8±2.3 mmol·l^–1). Following the 15 s test and the treadmill run, peak lactate values were 68.7 and 60.6% of the 60 s value respectively. Blood lactate production was greater (p<0.001) during the 15s test (0.470±0.128 mmol·l^–1·s^–1) than during the 60s test (0.184±0.042 mmol·l^–1·s^–1). Although blood lactate production was only nonsignificantly greater in AG, the amount of anaerobic work in the short tests was markedly greater (p<0.05-0.01) in AG than CG. Muscle fibre area (type II%) and serum testosterone were positively correlated (p<0.05) with blood lactate production in both short tests. Blood lactate elimination was greater (p<0.001) at the end of the active recovery phase than in the next (passive) phase. It is concluded that blood lactate production in prepubescent boys is related to serum testosterone level and muscle type II fibre area, indicating the role of maturation and training. Submaximal exercise is likely to increase blood lactate removal during recovery.     

Applying a mathematical model to training adaptation in a distance runner

This study investigated physiological and psychological correlates of the positive and negative components of a systems model in a well-trained male middle-distance runner. In the systems model, performance at any given point in time is seen as the difference between two antagonistic components, fitness and fatigue, which represent the positive and negative adaptation to training, respectively. Each component comprises a set of parameters unique to the individual, which were estimated by fitting model-predicted performance to performance measured weekly throughout a 12-week training period. The model fitness component was correlated with extrapolated VO_2max (ml.kg^–1.min^–1), running economy (RE) (VO₂ at 17 km.h^–1), and running speed (km.h^-1) at ventilatory threshold (VTRS). The model fatigue component was correlated with the fatigue subset of the profile of mood states (POMS). The fit between model and actual performance was significant (r²=0.92, P< 0.01). In the case of fitness, both VTRS (r=0.94, P=0.0001) and RE (r=–0.61, P=0.04) were significantly correlated with the model fitness component. There was also a moderate correlation between the fatigue subset of the POMS and the fatigue component (r=0.75, p< 0.05). In summary, this is the first time VTRS and the POMS have been used in an attempt to validate the model components. The findings of the present study support previous validation attempts using biochemical and hormonal markers of fitness and fatigue.     

Pathways of phosphate transport in chick jejunum

The effect of vitamin D₃ on intestinal phosphate (P_i) absorption was studied in everted sacs prepared from jejunum of either vitamin D-deficient (–D) or vitamin D-replete (+D) chicks. Vitamin D₃ stimulates the maximal velocity (V _max) of a mucosal active P_i transport mechanism from 125 to 314 nmol·min^–1·g^–1 tissue.K _m of this process remains virtually unchanged (–D: 0.15 mmol·l^–1; + D: 0.18 mmol·l^–1).Active P_i entry into the epithelium depends on extracellular Na⁺. Reduction of buffer Na⁺ reducesV _max in the + D group to 182 nmol·min^–1·g^–1 tissue but has no significant effect in the –D animals (V _max=105 nmol·min^–1·g^–1 tissue). In this group, the predominant effect of Na⁺ substitution is a shift ofK _m to 1.13 mmol·l^–1, whileK _m in the +D group is changed only to 0.53 mmol·l^–1.Transeptithelial P_i transport in the + D group involves the mucosal phosphate pump and hence an intracellular pathway, proceeding at a rate of 48 nmol·min^–1·g^–1 tissue. This is in contrast to –D P_i transfer (8 nmol·l^–1·g^–1 tissue) which is by a diffusional, Na⁺-insensitive, and presumably paracellular pathway.Transepithelial calcium transport (–D: 3.3 nmol·min^–1·g^–1; + D: 7.6 nmol·min^–1·g^–1 tissue) does not require the presence of extracellular Na⁺ and apparently involves pathways different from those of the P_i absorptive system.Presented in part at the Annual Meeting of the Austrian Biochemical Society, Salzburg, September 1978     

Lactate uptake by forearm skeletal muscles during repeated periods of short-term intense leg exercise in humans

We investigated the role of the forearm skeletal muscles in the removal of lactate during repeated periods of short-term intensive leg exercise, i.e. a force-velocity (FV) test known to induce a marked accumulation of lactate in the blood. The leg FV test was performed by seven untrained male subjects. Arterial and venous blood samples for determination of arterial ([la^–]_a) and venous ([la^–]_v) plasma lactate concentrations were concomitantly taken at rest before the test, during the FV test at the end of each period of intensive exercise just before the 5-min between-sprint recovery period, and after the completion of the test at 2, 4, 6, 8, 10, 15, and 20 min of the final recovery. The arteriovenous difference in concentration for plasma lactate ([la^–]_a–v) was determined for each blood sample. During the test, [la^–]_a and [la^–]_v increased significantly (P < 0.001;P < 0.001) with significantly higher values for [la^–]_a (P < 0.001). At the onset of the test, [la^–]_a–v became positive and increased up to a braking force of 6 kg, correlating significantly with [la^–]_a (r = 0.61,P < 0.001) with power (r = 0.58,P < 0.001) during the test. At the end of the test, [la^–]_a, [la^–]_v and [la^–]_a–v decreased (P < 0.001;P < 0.001;P < 0.001 respectively) but were still higher than the basal values after 20-min of passive recovery. In conclusion, forearm skeletal muscles would seem to have been involved in the removal of lactate from the blood during the leg FV test, with an increase in lactate uptake proportional to the increase in plasma lactate concentration and power.     

Serum hormones in male strength athletes during intensive short term strength training

Summary Training-induced adaptations in the endocrine system and strength development were investigated in nine male strength athletes during two separate 3-week intensive strength training periods. The overall amount of training in the periods was maintained at the same level. In both cases the training in the first 2 weeks was very intensive: this was followed by a 3rd week when the overall amount of training was greatly decreased. The two training periods differed only in that training period I included one daily session, while during the first 2 weeks of period II the same amount of training was divided between two daily sessions. In general, only slight and statistically insignificant changes occurred during training period I in mean concentrations of serum hormones examined or sex hormone-binding globulin as well as in maximal isometric leg extensor force. However, during training period II after 2 weeks of intensive strength training a significant decrease (P<0.05) was observed in serum free testosterone concentration [from 98.4 (SD 24.5) to 83.8 (SD 14.7) pmol · l^–1] during the subsequent week of reduced training. No change in the concentration of total testosterone was observed. This training phase was also accompanied by significant increases (P<0.05) in serum luteinizing hormone (LH) and cortisol concentrations. After 2 successive days of rest serum free testosterone and LH returned to (P<0.05) their basal concentrations. Training period II led also to a significant increase (P<0.05) [from 3942 (SD 767) to 4151 (SD 926) N] in maximal force. These findings suggest that in male strength athletes dividing the amount of training into smaller units may create more effective training stimuli leading to further strength development.     

Leptin as a marker of training stress in highly trained male rowers?

The purpose of this study was to investigate the potentially important role leptin may play during training monitoring in athletes. Twelve highly trained male rowers underwent a 3-week period of maximally increased training stress followed by a 2-week tapering period. Fasting blood was sampled after a rest day. Subjects also performed a maximal 2000-m rowing ergometer test before and after 3 weeks of heavy training, and after 2 weeks of tapering. Blood samples were obtained before, immediately after and after 30 min of recovery. Leptin concentrations were measured in duplicate by radioimmunoassay. Mean training time was about 100% higher during the heavy training period (17.5 h·week^–1) compared to the tapering period (8.9 h·week^–1). The 3-week heavy training period induced a significant reduction (P<0.05) in the fasting leptin concentration [from 2.5 (0.4) to 1.5 (0.4) ng·ml^–1]. Fasting plasma leptin was significantly increased by the end of the 2-week tapering period [2.0 (0.4) ng·ml^–1] but remained significantly lower compared to the pretraining value. Leptin levels were also significantly decreased only after the 2000-m rowing ergometer test performed at the end of the heavy training period. No differences in leptin concentrations were observed after other performance tests compared to their respective baseline values. In addition, fasting leptin concentration was significantly related to the weekly training time (r=–0.45; P=0.006). In conclusion, it appears that leptin is sensitive to the rapid and pronounced changes in training volume. A greater training time is associated with a lower leptin concentration in highly trained male rowers. It is suggested that it may be possible to direct typical rowing training by monitoring leptin status.     

Hormonal adaptations and modelled responses in elite weightlifters during 6 weeks of training

Summary The concentrations of serum testosterone, sex-hormone-binding-globulin (SHBG) and luteinizing hormone (LH) were examined throughout 1-year of training in six elite weightlifters. A systems model, providing an estimation of fatigue and fitness, was applied to records of training volume and performance levels in clean and jerk. The analysis focused on a 6-week training period during which blood samples were taken at 2-week intervals. A 4-week period of intensive training (period I) could be distinguished from the following 2-week period of reduced training (period II). During period I, decreases in serum testosterone (P<0.05) and increases in serum LH concentrations (P<0.01) were observed; a significant correlation (r=0.90,P<0.05) was also observed between the changes in serum LH concentration and in estimated fitness. The magnitude of LH response was not related to the change in serum androgens. On the other hand, the change in testosterone: SHBG ratio during period II was significantly correlated (r=0.97,P<0.01) to the LH variations during period I. These finding suggested that the LH response indicated that the decrease in testosterone concentration was not primarily due to a dysfunction of the hypothalamic-pituitary system control, and that the fatigue/fitness status of an athlete could have influenced the LH response to the decreased testosterone concentration. The negative effect of training on hormonal balance could have been amplified by its influence on the hypothalamic-pituitary axis. A decrease in physiological stress would thus have been necessary for the completion of the effect of LH release on androgenic activity.     

Hormonal responses to training and its tapering off in competitive swimmers: relationships with performance

During a winter training season, the effects of 12 weeks of intense training and 4 weeks of tapering off (taper) on plasma hormone concentrations and competition performance were investigated in a group of highly trained swimmers (n = 8). Blood samples were collected and the swimmers performed their speciality in competition at weeks 10 (mid-season), 22 (pre-taper) and 26 (post-taper). No statistically significant changes were observed in the concentrations of total testosterone (TT), non-sex hormone binding globulin-boundtestosterone (NSBT), cortisol (C), luteinising hormone, thyroid stimulating hormone, triiodothyronine, thyroxine plasma catecholamines, creatine kinase and ammonia during training and taper. Mid-season NSBT: C ratio and the amount of training were statistically related (r = 0.82,P < 0.05). Competition performance slightly declined during intense training [0.52 (SD 2.51) %, NS] and improved during taper [2.32 (SD 1.69)%,P < 0.01]. Changes in performance during training and taper correlated with changes in ratios TT: C (r = 0.86,P < 0.01andr = 0.81,P < 0.05, respectively) and NSBT: C (r = 0.77,P < 0.05 andr = 0.76,P < 0.05, respectively). In summary, these results showed that the monitored plasma hormones and metabolic indices were unaltered by 12 weeks of intense training and 4 weeks of taper. The TT: C and NSBT: C ratios, however, appeared to be effective markers of the swimmers' performance capacities throughout the training season.     

Day-to-day changes in oxygen uptake kinetics at the onset of exercise during strenuous endurance training

Summary The aim of this study was to assess the effect of strenuous endurance training on day-to-day changes in oxygen uptake (VO₂) on-kinetics (time constant) at the onset.of exercise. Four healthy men participated in strenuous training, for 30 min·day^–1, 6 days·week^–1 for 3 weeks. The VO₂ was measured breath-by-breath every day except Sunday at exercise intensities corresponding to the lactate threshold (LT) and the onset of blood lactate accumulation (OBLA) which were obtained before training. Furthermore, an incremental exercise test was performed to determine LT, OBLA and maximal oxygen uptake (VO_2max) before and after the training period and every weekend. The 30-min heavy endurance training was performed on a cycle ergometer 5 days·week^–1 for 3 weeks. Another six men served as the control group. After training, significant reductions of the VO₂ time constant for exercise at the pretraining LT exercise intensity (P<0.05) and at OBLA exercise intensity (P<0.01) were observed, whereas the VO₂ time constants in the control group did not change significantly. A high correlation between the decrease in the VO₂ time constant and training day was observed in exercise at the pretraining LT exercise intensity (r=–0.76; P<0.001) as well as in the OBLA exercise intensity (r= –0.91; P<0.001). A significant reduction in the blood lactate concentration during submaximal exercise and in the heart rate on-kinetics was observed in the training group. Furthermore, VO₂ at LT, VO₂ at OBLA and VO_2max increased significantly after training (P<0.05) but such was not the case in the control group. These findings indicated that within a few weeks of training a rapidly improved VO₂ on-kinetics may be observed. This may be explained. by some effect of blood lactate during exercise on VO₂ on-kinetics, together with significantly improved cardiovascular kinetics at the onset of exercise.     

Effects of power training on mechanical efficiency in jumping

The present study investigates the effects of power training on mechanical efficiency (ME) in jumping. Twenty-three subjects, including ten controls, volunteered for the study. The experimental group trained twice a week for 15 weeks performing various jumping exercises such as drop jumps, hurdle jumps, hopping and bouncing. In the maximal jumping test, the take-off velocity increased from 2.56 (0.24) m·s^–1 to 2.77 (0.18) m·s^–1 (P<0.05). In the submaximal jumping of 50% of the maximum, energy expenditure decreased from 660 (110) to 502 (68) J·kg^–1·min^–1 (P<0.001) while, simultaneously, ME increased from 37.2 (8.4)% to 47.4 (8.2)% (P<0.001). Some muscle enzyme activities of the gastrocnemius muscle increased during the training period: citrate synthase from 35 (8) to 39 (7) mol·g^–1 dry mass·min^–1 (P<0.05) and -hydroxyacyl CoA dehydrogenase from 21 (4) to 23 (5) mol·g^–1 dry mass·min^–1 (P<0.05), whereas no significant changes were observed in phosphofructokinase and lactate dehydrogenase. In the control group, no changes in ME or in enzyme activities were observed. In conclusion, the enhanced performance capability of 8% in maximal jumping as a result of power training was characterized by decreased energy expenditure of 24%. Thus, the increased neuromuscular performance, joint control strategy, and intermuscular coordination (primary factors), together with improved aerobic capacity (secondary factor), may result in reduced oxygen demands and increased ME.     

The role of anaerobic ability in middle distance running performance

Summary The purpose of this study was to assess the relationship between anaerobic ability and middle distance running performance. Ten runners of similar performance capacities (5 km times: 16.72, SE 0.2 min) were examined during 4 weeks of controlled training. The runners performed a battery of tests each week [maximum oxygen consumption (VO_2max), vertical jump, and Margaria power run] and raced 5 km three times (weeks 1, 2, 4) on an indoor 200-m track (all subjects competing). Regression analysis revealed that the combination of time to exhaustion (TTE) during theVO_2max test (r ²=0.63) and measures from the Margaria power test (W·kg^–1,r ²=0.18 ; W,r ²=0.05) accounted for 86% of the total variance in race times (P<0.05). Regression analysis demonstrated that TTE was influenced by both anaerobic ability [vertical jump, power (W·kg^–1) and aerobic capacity (VO_2max, ml·kg^–1·min^–1)]. These results indicate that the anaerobic systems influence middle distance performance in runners of similar abilities.     

The effect of ski training at altitude and racing on pituitary,adrenal and testicular function in men

Summary The effect of similar prolonged exercise on hormonal changes was studied at sea level and at moderate altitude. Four cross-country skiers participated in a 30-km race and five biathlonists in a 20-km race at sea level in Finland and during altitude training and racing at 1650 m in Les Saisies, France. Venous blood samples were taken at both altitudes before the race between 0800 and 0900 hours and 25–35 min after the race. Resting blood samples were also taken before and after the altitude training and the period of racing. Serum testosterone concentration was higher before the race at altitude than at sea level (19%, P<0.02), and 30 min after the race growth hormone (GH) concentration was higher at sea level than at moderate altitude (P<0.002). There were not significant differences in serum luteinising hormone between the altitudes. Serum cortisol concentration was higher after the altitude training and the period of racing than before (P<0.02) but no difference was observed in testosterone. We concluded, that since the profiles of the anabolic-catabolic hormone concentrations measured are indicators of the performance level of athletes, our data indicated that to follow them during altitude training could be beneficial in optimizing training programme for individual athletes. We also concluded, that the lower GH concentration after racing at moderate altitude may have been a consequence of decreased racing speed and/or increased physical performance.     

Serum hormones during prolonged training of neuromuscular performance

Summary The effects of a 24-weeks' progressive training of neuromuscular performance capacity on maximal strength and on hormone balance were investigated periodically in 21 male subjects during the course of the training and during a subsequent detraining period of 12 weeks. Great increases in maximal strength were noted during the first 20 weeks, followed by a plateau phase during the last 4 weeks of training. Testosterone/cortisol ratio increased during training. During the last 4 weeks of training changes in maximal strength correlated with the changes in testosterone/cortisol (P<0.01) and testosterone/SHBG (P<0.05) ratios. During detraining, correlative decreases were found between maximal strength and testosterone/cortisol ratio (P<0.05) as well as between the maximal strength and testosterone/SHBG ratio (P<0.05). No statistically significant changes were observed in the levels of serum estradiol, lutropin (LH), follitropin (FSH), prolactin, and somatotropin. The results suggest the importance of the balance between androgenic-anabolic activity and catabolizing effects of glucocorticoids during the course of vigorous strength training.     

Cortisol,testosterone, and insulin action during intense swimming training in humans

An increase in the amounts of circulating plasma cortisol or a decrease in testosterone can result in whole-body insulin resistance. The purpose of this study was to determine if the increase in cortisol and/or decrease in testosterone concentrations commonly evident with intense endurance training is associated with insulin resistance. Male (n = 9) and female (n = 10) swimmers were examined during the off-season, after 9 weeks (9 WKS) of training averaging 5,500 m·day^–1 and after an additional 9 weeks (18 WKS) of training averaging 8,300 m·day^–1. Resting plasma cortisol concentration was (P <– 0.05) higher in the women compared to the men at 9 WKS; values were not significantly different between genders at 18 WKS. Plasma testosterone concentration decreased significantly (P <– 0.05) in the men at 9 and 18 WKS, but did not change in the women. Whole-body insulin action, as determined by insulin and glucose responses during a 120 min, 75-g oral glucose tolerance test, did not change with training in either the men or women. These data indicated that plasma testosterone concentration can decrease in male swimmers during intense endurance training; this alteration does not affect whole-body insulin action. There would also appear to be a gender-specific response of plasma cortisol to endurance training, which does not influence insulin action.