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1.
目的:探索转化生长因子β1(transforming growth factor-β1,TGF-β1)诱导三维培养肺癌细胞发生上皮-间充质转化(epithelial-mesenchymal transition,EMT)及对顺铂抵抗的相关机制.方法:用荧光倒置显微镜、扫描电镜及激光共聚焦观察人非小细胞肺癌细胞系95D在... 相似文献
2.
Model of the early development of diffuse gastric cancer in E-cadherin mutation carriers and its implications for patient screening 总被引:8,自引:0,他引:8
Carneiro F Huntsman DG Smyrk TC Owen DA Seruca R Pharoah P Caldas C Sobrinho-Simões M 《The Journal of pathology》2004,203(2):681-687
Hereditary diffuse gastric cancer (HDGC) is a familial cancer syndrome caused, in 30-40% of cases, by germline mutations of the E-cadherin/CDH1 gene. The presence of clinically undetectable early gastric cancers has been previously reported in ten of ten prophylactic gastrectomies from germline E-cadherin mutation carriers. In the present study, detailed maps of the distribution of invasive cancers in nine of these ten stomachs were produced and precursor lesions of HDGC searched for. The nine gastrectomy specimens contained from 1 to 161 foci of early diffuse gastric cancer, occupying 0.005-2.96% of the gastric mucosa. Seven specimens contained focal in situ signet ring carcinoma. Pagetoid spread of signet ring cells was observed beneath the epithelial lining of gastric foveolae/glands. Helicobacter pylori organisms and associated pathology were absent from all cases. Two-dimensional maps of the gastrectomy specimens revealed lesions throughout the gastric mucosa without evidence of antral clustering. The distribution and size of the cancers in the gastrectomy specimens indicate that standard endoscopic screening with random or geographically targeted biopsies is unlikely to provide sufficiently sensitive clinical screening for at-risk individuals. An in situ precursor of signet ring carcinoma was identified and a model for neoplastic progression in the setting of HDGC is proposed. 相似文献
3.
A three-dimensional cell coculture method is presented where cancer cells can be maintained alone or combined with other cell types in longterm culture in order to reconstitute some of the interactions between the different cell elements in tumors in vivo. The cells are accumulated by centrifugation to form nodules which are cultivated on a semi-solid agar medium at medium/air interface. The nodules are not mere cell aggregates, they are able to develop morphological and functional differentiation as well as tissue-like membrane junctions. Studies on short-term and long-term effects of anticancer treatments are possible and their long-term regrowth can be obtained. Especially, in nodules containing cell mixtures, the localization of the different cell types can be determined and their specific differentiation. An example showing stroma-like formations and collagen production in breast cancer cell and breast fibroblast containing nodules is presented. 相似文献
4.
Randi M. Williams Kimberly M. Davis George Luta Sara N. Edmond Caroline S. Dorfman Marc D. Schwartz John Lynch Chiledum Ahaghotu Kathryn L. Taylor 《Patient education and counseling》2013