人参皂苷Rg3对宫颈癌荷瘤小鼠治疗作用的实验研究

目的探讨人参皂苷Rg3对宫颈癌荷瘤小鼠的治疗作用。方法使用雄性5周龄Balb/c小鼠65只,随机取10只作为正常对照组,其它55只均在右前上肢腋窝皮下接种U14小鼠宫颈癌细胞作为实验组;从接种之日起每日称量体重和观察测量肿瘤体积,5 d后瘤体积达100~120 mm3时随机分为4组,即肿瘤对照组10只、人参皂苷Rg3高剂量(10 mg/kg)组15只、人参皂苷Rg3低剂量(5 mg/kg)组15只、阳性药(环磷酰胺50 mg/kg)组15只,然后开始治疗,每天灌胃给药治疗1次,共治疗14次;于治疗结束后第3天处死小鼠取肿瘤组织。采用酶联免疫法和免疫组织化学方法检测各组癌基因ras和抑癌基因PTEN的蛋白含量及蛋白阳性表达强度。结果与正常对照组比较,肿瘤对照组的癌基因ras的表达水平明显增高、抑癌基因PTEN的表达水平明显降低,两者差异均有统计学意义(P0.01)。人参皂苷Rg3组随剂量增加癌基因ras的表达水平显著降低,与正常对照组比较差异有统计学意义(P0.01);抑癌基因PTEN的表达水平明显升高,但与对照组比较差异无统计学意义(P0.05);阳性药组与人参皂苷Rg3高剂量组差异无统计学意义(P0.05)。结论人参皂苷Rg3高、低剂量组均对U14宫颈癌细胞有明显抑瘤作用,其作用机制可能是人参皂苷Rg3参与了癌基因和抑癌基因的调控。     

蚕蛹蛋白缓解运动性疲劳的实验研究

[目的]研究蚕蛹蛋白的缓解运动性疲劳作用.[方法]采用雄性ICR小鼠,蚕蛹蛋白按33.3、66.7、100.0ml/kg.bw连续灌胃30d,测定小鼠负重游泳时间、血乳酸、血清尿素氮和肝糖原含量、红细胞数目和血红蛋白含量、常压耐缺氧时问以及创伤愈合时问.[结果]3个蚕蛹蛋白组小鼠负重游泳时间显著长于空白对照组(P<0.05).3个蚕蛹蛋白组小鼠游泳后0min血乳酸含量低于对照组(P<0.05, P<0.01);8.4g/kg.bw蚕蛹蛋白组小鼠游泳后213min血乳酸含量低于对照组(P<0.01);8.4g/kg.bw和12.5g/kg.bw蚕蛹蛋白组小鼠3个时点血乳酸曲线下面积低于对照组,差异有统计学意义(P<0.05, P<0.01).4.2g/kg.bw蚕蛹蛋白剂量组肝糖原含量显著高于空白对照组(P<0.05).4.2g/kg.bw蚕蛹蛋白组小鼠红细胞数量和血红蛋白含量高于对照组(P<0.05).8.4g/kg.bw蚕蛹蛋白组小鼠耐缺氧时间长于对照组(P<0.05).4.2g/kg.bw蚕蛹蛋白组小鼠伤口愈合天数少于对照组(P<0.05).[结论]蚕蛹蛋白具有缓解运动性疲劳的作用.     

吡咯喹啉醌对模拟急性高原暴露小鼠的抗缺氧和抗疲劳作用的研究

目的探讨吡咯喹啉醌(pyrroloquinolinequinone,PQQ)对模拟急性高原暴露小鼠的抗缺氧和抗疲劳的作用效果。方法常氧条件下,选取6～8 w雄性昆明小鼠,随机分为常氧对照、5 mg/kg PQQ、10mg/kg PQQ和20mg/kg辅酶Q10(Q10)共4组,采用常压密闭缺氧实验评价小鼠的耐缺氧能力。低氧条件下,选取6～8w雄性昆明小鼠,随机分为低氧对照组、5 mg/kg PQQ组、10mg/kg PQQ组和20mg/kg Q10组共4组,采用低压氧舱模拟海拔10 km评价小鼠的减压缺氧耐受力;模拟海拔4 km低氧负重游泳力竭实验评价小鼠的抗疲劳能力。同时检测低氧游泳力竭小鼠的血乳酸、肝糖原、血清超氧化物岐酶(superoxide dismutase, SOD)、血清丙二醛(malondialdehyde, MDA)、血清尿素(urea)等指标变化。结果与常氧对照组比,PQQ组可明显提高小鼠常压密闭缺氧的生存时间(P<0.05)。与低氧对照组比,PQQ组可明显提高10 km急性减压缺氧的存活时间(P<0.05)和低氧游泳力竭时间(P<0.05);减少...     

人参三醇组皂苷对运动疲劳大鼠血糖、血乳酸、血尿素氮的影响

目的探讨不同剂量人参三醇组皂苷对运动疲劳大鼠血糖、血乳酸、血尿素氮的影响。方法成熟Wistar大鼠,训练前每日经口灌胃给予PTS高(100.0mg/kg bw)、中(50.0mg/kg bw)、低(25.0mg/kg bw)3个剂量,另设安静对照组、空白训练组(灌胃给予生理盐水)及甲基睾酮组(甲基睾酮水混悬液2.4 mg/kg bw灌胃)。游泳训练7w,观察人参三醇组皂苷对运动疲劳大鼠血糖、血乳酸、血尿素氮的影响。结果甲基睾酮组、人参三醇组皂苷高、中剂量组与空白训练组比较大鼠力竭游泳时间延长,血糖升高差异有统计学意义(均P<0.05);甲基睾酮组、人参三醇组皂苷高剂量组与空白训练组比较血乳酸、血尿素氮浓度降低差异有统计学意义(P<0.05)。结论 PTS对运动疲劳大鼠血糖、血乳酸、血尿素氮有一定影响,能延缓疲劳的产生。     

抗疲劳中药制剂对游泳小鼠抗疲劳效果观察

目的观察抗疲劳中药制剂的抗疲劳效果。方法抗疲劳中药制剂由人参、刺五加、葛根、黄芪、枸杞等组成,按低、中、高剂量灌胃每天1次,连续2周,以负重游泳的方法制备疲劳模型,观察小鼠力竭性游泳时间及血糖、乳酸、尿素氮和肝糖原水平,并与正常对照组和游泳对照组比较。结果抗疲劳中药制剂3.04、.5 mg.kg-1组动物力竭性游泳时间比游泳对照组分别延长45.3%和47.7%,差异均有显著性(P<0.05)。抗疲劳中药制剂3.04、.5 mg.kg-1组血糖含量分别为(5.3±0.4)mmol.L-1和(5.4±0.5)mmol.L-1,均高于游泳对照组〔(4.7±0.7)mmol.L-1〕,低于正常对照组〔(6.2±0.5)mmol.L-1〕,差异均有显著性(P<0.05);抗疲劳中药制剂3个剂量组肝糖原水平均显著高于游泳对照组(P<0.05,P<0.01),血尿素氮水平下降,血乳酸水平略有升高。结论本抗疲劳中药制剂能有效维持游泳动物血糖、肝糖原水平,抑制蛋白质分解,具有一定的抗疲劳作用。     

析因分析绿茶对被动吸烟小鼠力竭游泳能力的影响

[目的]研究绿茶对被动吸烟小鼠力竭游泳能力的影响. [方法]采用2×2析因设计,40只昆明小鼠随机分成对照组A、被动吸烟组B、用茶组C和吸烟用茶组D,每组10只(n=10).B、D组每天被动吸烟2次,1 h/次(6支/h),每天C、D组饮用绿茶茶汤(2.5%),A、B组饮用白开水均自由饮用,共30 d.d 31进行力竭游泳试验,记录力竭运动时间;后断头采血,测定血尿素氮(Blood urea nitrogen,BUN)、血红蛋白(hemoglobin,Hb)和血乳酸(blood lactic acid,BLA)含量. [结果]被动吸烟小鼠BUN和BLA含量均显著增加(P＜0.01),力竭游泳时间显著缩短(P＜0.01);与B组比较,吸烟用茶组小鼠力竭游泳时间显著延长(P＜0.01),BUN和BLA含量显著降低(P＜0.01),Hb含量降低不明显(P＞0.05).经统计学分析,绿茶与被动吸烟间有交互作用. [结论]绿茶能改善与力竭游泳能力相关的某些生理指标,显著提高被动吸烟小鼠的力竭游泳时间,缓解被动吸烟的伤害.     

蚕蛹蛋白对模拟高原环境下小鼠的抗疲劳作用

目的探讨蚕蛹蛋白对缺氧小鼠的抗疲劳作用,为其抗疲劳作用在高原的应用提供实验室依据。方法雄性昆明种小鼠80只(n=20),按体重随机分为4组,分别为缺氧对照组、蚕蛹蛋白低剂量组(0.5g/kg)、中剂量组(2.5g/kg)和高剂量组(5g/kg)。各组小鼠均在模拟5000m高原环境中饲养,连续灌胃10d后测小鼠力竭游泳时间、肝糖原含量、血乳酸及血糖水平。结果与缺氧对照组比较,蚕蛹蛋白显著延长了小鼠力竭游泳时间、增加肝糖原含量和降低血乳酸曲线下面积(P﹤0.01),其中以中剂量组效果最佳,而蚕蛹蛋白对血糖水平没有显著影响。结论蚕蛹蛋白对缺氧小鼠具有延缓疲劳产生和加速疲劳消除的作用。     

5'-核苷酸抗疲劳作用研究

[目的]研究5'-核苷酸抗疲劳作用。[方法]160只SPF级雄性ICR小鼠,随机分为4组,设立空白对照组、5'-核苷酸低、中、高剂量组(分别为0.04g/kg.bw、0.16g/kg.bw和0.64g/kg.bw)。30d后,测定各组小鼠负重游泳时间、血清尿素氮、肝糖原、血乳酸等指标。[结果]5'-核苷酸中、高剂量组均可显著延长小鼠负重游泳时间(P﹤0.05),并减少小鼠不负重游泳前及游泳后0min和20min3个时间点血乳酸测定曲线下的面积(P﹤0.05);5'-核苷酸各剂量组均可显著降低小鼠不负重游泳90min后血清尿素氮水平(P﹤0.05)。[结论]5'-核苷酸具有缓解体力疲劳功能。     

某伤科中成药抗疲劳作用的研究

[目的]研究某伤科中成药对实验性动物抗疲劳作用的影响。[方法]将昆明小鼠随机分组,高、中、低剂量组分别经口给予不同浓度(4、2、1g/kg)受试样品,阴性对照组则给予同体积生理盐水,连续给药15d后进行负重游泳试验,记录游泳时间并测定血清尿素氮,观测运动后血清乳酸升高和运动休息后血清乳酸的消除情况。[结果]高剂量组与对照组相比,小鼠游泳时间显著延长(P<0.01);小鼠运动后血清尿素氮含量显著降低(P<0.01);血清乳酸含量升高幅度无明显变化,但其消除幅度明显加大(P<0.05)。中剂量组与阴性对照组相比较,小鼠游泳时间变化不大,但小鼠运动后血清尿素氮含量显著降低(P<0.01),血清乳酸含量升高幅度无明显变化,但其消除幅度明显加大(P<0.05)。低剂量组与阴性对照组相比较各指标无明显变化。[结论]某伤科中成药具有缓解体力疲劳的功能。     

复合营养补充剂对小鼠的抗疲劳作用

目的研究复合营养补充剂（由蜂花粉、人参皂苷、B族维生素组成）的抗疲劳作用,为抗疲劳功能食品的开发提供实验依据。方法用不同剂量的复合营养补充剂给小鼠灌胃10d,测定负重游泳力竭时间、游泳后24hMorris水迷宫逃避潜伏期、游泳后肝糖原和肌糖原含量,以及游泳后24h血清抗氧化能力。结果复合营养补充剂低、中、高剂量组及空白对照组的平均负重游泳力竭时间分别为（18.1±4.0）min、（28.7±6.9）min、（15.3±5.9）min、（10.1±1.8）min,各剂量组均明显长于空白对照组（P〈0.05,P〈0.01）,中剂量组明显长于低、高剂量组（P〈0.05）;中剂量组逃避潜伏期为（22.6±14.0）s,明显短于空白对照组的（40.2±12.6）s,游泳后各剂量组的肝糖原和肌糖原含量均明显高于空白对照组（P〈0.05,P〈0.01）;中剂量组血清TAOC水平明显高于空白对照组（P〈0.01）、血清MDA水平明显低于空白对照组（P〈0.05）,各剂量组血清抑制羟自由基能力明显高于空白对照组（P〈0.05,P〈0.01）。结论复合营养补充剂具有抗疲劳作用,其机制可能与增加糖原储备和提高机体抗氧化能力有关。     

支链氨基酸提高大鼠游泳耐力作用探讨

目的 :　探讨支链氨基酸 (BCAA)对提高大鼠运动耐力的作用。方法 :　取雄性Wistar大鼠 2 1只 ,随机分为三组 :正常组、游泳对照组及游泳补充 5% BCAA饲料组。两个游泳组每天游泳训练 1 h,1 0 d后 ,游泳 6h,观察游泳大鼠的存活率 ,测定血乳酸和尿素氮水平 ,血清和骨骼肌乳酸脱氢酶 (LDH)活力 ,线粒体脂质过氧化物 (LPO)水平和线粒体膜的粘度系数。另取昆明种小鼠 ,随机分为三组 ,用实验一相同的饲料喂养 ,两周后 ,于每只小鼠尾静脉注射 15N-甘氨酸 (15N-Gly) 1 .0 mg,注射后 和 组立即游泳 ,分别于注射后的 1、2、3及 4h,测定各组骨骼肌蛋白质中15N-甘氨酸 (15N- Gly)的丰度。结果 :　BCAA可明显提高大鼠游泳存活率 ,抑制游泳运动后大鼠的血乳酸浓度、LDH活力、骨骼肌 LPO的升高幅度 ,抑制骨骼肌 LDH活力和膜流动性下降的趋势。并且 BCAA还可增加 15N- Gly在骨骼肌蛋白质中的滞留时间。结论 :　BCAA可改善运动后骨骼肌线粒体功能 ,改善运动性疲劳 ,提高大鼠的运动耐力     

固相萃取-超高效液相色谱法同时测定人参中11种人参皂苷的含量

目的:建立了人参中11种人参皂苷同时检测的超高效液相色谱分析方法。方法:采用Waters Ac-quity UPLC BEH C18色谱柱(1.7μm,50 mm×2.1 mm)、乙腈-水流动相(流速0.5 ml/min)柱温30℃、检测波长:203 nm的色谱条件,同时分离测定人参皂苷R1、Rg1、Re、Rf、Rh1、Rg2、Rb1、Rc、Rb2、Rd、Rg3。样品以水饱和正丁醇为提取溶剂,经大孔吸附树脂柱除去基质干扰,取2μl注入UPLC。结果:11种人参皂苷在10 mg/L～100 mg/L范围内呈良好的线性关系,方法定量限为0.01%,加标回收率在90.9%～101.8%,所测定的RSD在1.89%～4.23%之间。结论:方法灵敏、快速,结果准确可靠。     

支链氨基酸对运动大鼠心肌能源物质代谢的影响

目的：探讨在运动状态下，支链氨基酸（ＢＣＡＡ）对心肌能源物质代谢的影响，为进一步研究ＢＣＡＡ对心脏功能的作用提供依据。方法：采用Ｗｉｓｔａｒ大鼠游泳运动模型，观察运动期间补充支链氨基酸（ＢＣＡＡ）对心肌中氨基酸、游离脂肪酸和糖原代谢的影响。结果：游泳运动使心肌中Ａｓｐ、Ａｌａ、Ｃｙｓ、Ｖａｌ显著增加，Ｇｌｎ、Ｇｌｕ降低，除Ａｌａ外，血浆中许多氨基酸都有不同程度的升高，其中Ｔａｕ、Ｇｌｎ、Ｃｙｓ、Ｉｌｅ、Ｐｈｅ达到了显著性；硬脂酸（Ｃ１８０）升高，花生四烯酸（Ｃ２０４）降低。心肌糖原升高，骨骼肌和肝脏糖原含量降低。补充ＢＣＡＡ后，心肌和血浆中的ＢＣＡＡ显著增加，同时心肌中Ａｓｐ、Ｇｌｎ、Ａｌａ显著高于运动对照组，血清中与糖原异生有关的氨基酸Ｇｌｎ、Ｇｌｕ、Ａｌａ显著降低；肌糖原升高；Ｃ１８０降低。结论：运动条件下，增加ＢＣＡＡ的氧化，使心肌中的一些重要的氨基酸含量增加；促进了糖原异生或节约了肌糖原，可能有利于脂肪的氧化。     

Influence of branched-chain amino acid supplementation on urinary protein metabolite concentrations after swimming

OBJECTIVE: The influence of branched-chain amino acid (BCAA) supplementation on urinary urea nitrogen, hydroxyproline (HP), and 3-methylhistidine (3MH) concentrations after 25 min of breast stroke exercise (65-70% maximum heart rate reserved, 65-70% HRRmax) followed by a 600 m crawl stroke competition was investigated in a double-blind, counter-balanced study. METHODS: Male university students (19-22 years old) majoring in physical education participated in the study. Based on the previous swimming time of a 600 m crawl stroke, the participants were divided into two groups: placebo (n = 9, BMI = 24.2 +/- 2.1 kg/m2; 12 g of glucose/day; in capsules) and BCAA (n = 10, BMI = 22.7 +/- 1.5 kg/m2; 12 g of BCAAs/day; in capsules: leucine 54%, isoleucine 19%, valine 27%) groups. The participants maintained a regular dietary intake (except the prescribed breakfast on day 15) and exercise activity at a moderate/low intensity (60-70% HRRmax, swimming and rowing, approximately 1.5 hour/day) during the 15-day study. A prescribed exercise program was performed on day 15. Urinary and blood samples were collected before, during, and after the prescribed exercise for the measurements of the urinary urea nitrogen, HP, and 3MH concentrations in urine, as well as the glucose, lactate, glutamine, alanine, and BCAA concentrations in plasma. RESULTS: Two weeks of dietary supplementation did not induce any changes in the plasma glucose and total BCAA concentrations of either group, nor in the urinary urea nitrogen, HP, and 3MH concentrations in urine. On day 15, after 25 min of breast stroke exercise and a 600 m crawl stroke competition, plasma glucose concentration decreased significantly (p < 0.05) whereas plasma lactate concentration increased significantly (p < 0.05) in both groups. The exercise program prescribed in the study did not affect urinary urea nitrogen, HP, and 3MH concentrations. Twenty hours after the competition, however, a significant increase in the concentrations of urinary urea nitrogen, HP, and 3MH was found in the placebo group (p < 0.05), but not in the BCAA group. CONCLUSIONS: The results obtained in this study suggest that swimming induced muscle proteolysis was prevented by BCAA supplementation. The mechanism could be attributed to the availability of ammonia provided by the oxidation of supplemented BCAAs during exercise.     

Effects of Diets Supplemented with Branched-Chain Amino Acids on the Performance and Fatigue Mechanisms of Rats Submitted to Prolonged Physical Exercise

This study aimed to determine the effects of diets chronically supplemented with branched-chain amino acids (BCAA) on the fatigue mechanisms of trained rats. Thirty-six adult Wistar rats were trained for six weeks. The training protocol consisted of bouts of swimming exercise (one hour a day, five times a week, for six weeks). The animals received a control diet (C) (n = 12), a diet supplemented with 3.57% BCAA (S1) (n = 12), or a diet supplemented with 4.76% BCAA (S2) (n = 12). On the last day of the training protocol, half the animals in each group were sacrificed after one hour of swimming (1H), and the other half after a swimming exhaustion test (EX). Swimming time until exhaustion was increased by 37% in group S1 and reduced by 43% in group S2 compared to group C. Results indicate that the S1 diet had a beneficial effect on performance by sparing glycogen in the soleus muscle (p < 0.05) and by inducing a lower concentration of plasma ammonia, whereas the S2 diet had a negative effect on performance due to hyperammonemia (p < 0.05). The hypothalamic concentration of serotonin was not significantly different between the 1H and EX conditions. In conclusion, chronic BCAA supplementation led to increased performance in rats subjected to a swimming test to exhaustion. However, this is a dose-dependent effect, since chronic ingestion of elevated quantities of BCAA led to a reduction in performance.     

Influence of Branched-Chain Amino Acid Supplementation on Urinary Protein Metabolite Concentrations after Swimming

Objective: The influence of branched-chain amino acid (BCAA) supplementation on urinary urea nitrogen, hydroxyproline (HP), and 3-methylhistidine (3MH) concentrations after 25 min of breast stroke exercise (65–70% maximum heart rate reserved, 65–70% HRRmax) followed by a 600 m crawl stroke competition was investigated in a double-blind, counter-balanced study.

Methods: Male university students (19–22 years old) majoring in physical education participated in the study. Based on the previous swimming time of a 600 m crawl stroke, the participants were divided into two groups: placebo (n = 9, BMI = 24.2 ± 2.1 kg/m²; 12 g of glucose/day; in capsules) and BCAA (n = 10, BMI = 22.7 ± 1.5 kg/m²; 12 g of BCAAs/day; in capsules: leucine 54%, isoleucine 19%, valine 27%) groups. The participants maintained a regular dietary intake (except the prescribed breakfast on day 15) and exercise activity at a moderate/low intensity (60–70% HRRmax, swimming and rowing, ～1.5 hour/day) during the 15-day study. A prescribed exercise program was performed on day 15. Urinary and blood samples were collected before, during, and after the prescribed exercise for the measurements of the urinary urea nitrogen, HP, and 3MH concentrations in urine, as well as the glucose, lactate, glutamine, alanine, and BCAA concentrations in plasma.

Results: Two weeks of dietary supplementation did not induce any changes in the plasma glucose and total BCAA concentrations of either group, nor in the urinary urea nitrogen, HP, and 3MH concentrations in urine. On day 15, after 25 min of breast stroke exercise and a 600 m crawl stroke competition, plasma glucose concentration decreased significantly (p < 0.05) whereas plasma lactate concentration increased significantly (p < 0.05) in both groups. The exercise program prescribed in the study did not affect urinary urea nitrogen, HP, and 3MH concentrations. Twenty hours after the competition, however, a significant increase in the concentrations of urinary urea nitrogen, HP, and 3MH was found in the placebo group (p < 0.05), but not in the BCAA group.

Conclusions: The results obtained in this study suggest that swimming induced muscle proteolysis was prevented by BCAA supplementation. The mechanism could be attributed to the availability of ammonia provided by the oxidation of supplemented BCAAs during exercise.     

人参皂苷Rg3对大鼠子宫内膜异位症组织中血管内皮生成因子影响的研究

目的 观察人参皂苷Rg3（Rg3）对大鼠子宫内膜异位症（endometriosis，EMs）组织中血管内皮生成因子（vascular endothelial growth factors，VEGF）影响和抗血管生成作用。方法 参照Jones方法建立Wistar大鼠EMs动物模型，3周后将建模成功的大鼠随机分为空白对照、孕三烯酮和Rg3各2．4，6及8周组,共12组。用SABC免疫组化法测定异位灶组织中VEGF的表达。结果 Rg3治疗大鼠EMs模型2，4，6，8周后，异位组织中VEGF的表达明显低于对照组，差异有显著意义（P〈0．05）；而孕三烯酮治疗相同时间后与对照组相比，VEGF表达无明显改变，差异无显著意义（P〉0．05）；Rg3组随治疗时间延长，VEGF明显减少（P〈0．05），而对照组和孕三烯酮组无此表现。结论 Rg3对大鼠子宫内膜异位组织中VEGF表达和新生血管生成均有抑制作用。Rg3有望成为子宫内膜异位抗血管生成治疗新药。     

Optimal ratio of individual branched-chain amino acids in total parenteral nutrition of injured rats.

In this study, we investigated the optimal ratio of individual branched-chain amino acids (BCAA) in a balanced amino acid infusion in laparotomized rats. The total BCAA contents of four amino acid infusions were fixed at 31% of total amino acids. The weight ratios of individual BCAA (isoleucine:leucine:valine) in the solutions were 1:0.5:1, 1:1:1, 1:2:1, and 1:4:1, respectively. The laparotomized rats were infused with about 140 mg (experiment 1) and 100 mg (experiment 2) of nitrogen and 10 g of glucose daily for 7 days. In both experiments, no marked difference was observed in the mean cumulative 7-day nitrogen balance and the urinary 3-methyl-histidine levels of all the groups. The BCAA concentrations and the molar ratios of individual BCAA in plasma were disarranged by the infusion of the 1:0.5:1 and 1:4:1 solutions. The infusion of the 1:1:1 and 1:2:1 solutions tended, however, to allow the values to approach the preinfusion values. These results suggest that the optimal ratio of individual BCAA in an amino acid infusion lies between 1:1:1 and 1:2:1 for this injured rat model in total parenteral nutrition.     

Effect of Oral Branched‐Chain Amino Acid Supplementation on Postoperative Blood Lactate Levels in Patients Undergoing Pancreatoduodenectomy

Background: Elevations in blood lactate levels have been associated with poor postoperative outcome. The aim of the present study was to determine if preoperative supplementation with branched‐chain amino acids (BCAA) decreases postoperative blood lactate levels in patients undergoing pancreatoduodenectomy. Methods: The cases of 223 consecutive patients who underwent pancreatoduodenectomy were retrospectively reviewed. Postoperative blood lactate levels in patients who were treated with BCAA supplementation before pancreatoduodenectomy (December 2011–December 2014) were compared with levels in patients who were not pretreated (January 2008–November 2011). Results: Postoperative lactate levels were significantly lower in patients who received preoperative BCAA supplementation than in patients who did not (2.6 vs 3.1 mmol/L, P = .005), although preoperative blood lactate levels were statistically equivalent between the groups. In the univariate analysis, preoperative BCAA supplementation, preoperative lactate levels, operation time, and postoperative glucose levels were associated with postoperative lactate levels. A multiple regression analysis was performed among the 4 factors, and the preoperative use of BCAA supplementation was independently correlated with postoperative lactate levels (P = .046). Conclusions: Preoperative BCAA supplementation decreased postoperative blood lactate levels. These results suggest that preoperative BCAA supplementation may help patients recover after surgery.     

人参皂苷Rg1通过NF-κB阻断缺氧诱导卵巢癌SKOV3细胞EMT

目的 探讨人参皂苷Rg1对卵巢癌SKOV3细胞上皮间质转化的影响及机制.方法 确定人参皂苷Rg1对SKOV3细胞生长的影响,构建缺氧诱导上皮间质转化(EMT)模型,观察细胞形态变化,检测E-cadherin,vimentin及核因子KappaB(NF-κB)蛋白的表达.结果 不同浓度的人参皂苷Rg1处理SKOV3细胞,超过40μg/mL的Rg1培养24h时对细胞生长稍有抑制,培养48h和72h时对细胞生长无明显抑制.缺氧诱导48h,SKOV3细胞呈现纺锤形、松散排列的形态,E-cadherin蛋白表达消失,vimentin蛋白表达增强.40μg/mL人参皂苷Rg1处理缺氧诱导的SKOV3细胞48h,细胞形态变化被部分逆转.E-cadherin蛋白表达恢复,vimentin蛋白表达被抑制,同时,NF-κB蛋白的表达与E-cadherin呈相反的趋势.结论 人参皂苷Rg1可能通过调控NF-κB抑制缺氧诱导卵巢癌SKOV3细胞的EMT.