Deletion (21)(q21.2q22.12) as a sole clonal cytogenetic abnormality in a lobular capillary hemangioma of the nasal cavity

A clonal deletion (21)(q21.2q22.12) was detected as a sole cytogenetic abnormality in a lobular capillary hemangioma (pyogenic granuloma) of the nasal cavity. This finding supports a neoplastic, rather than reactive, nature for this lesion. To our knowledge, these rare lesions have not previously been studied by cytogenetics.     

Abdominal situs inversus in a Holstein calf

Situs inversus is a rare congenital anomaly wherein the normal, left-right organ location is inverted. The anatomical inversion (heterotaxy) is usually detected in routine radiography or other medical interventions. A 5-month-old Holstein heifer calf was identified as a suspected situs inversus totalis during abdominal surgery. Following surgery the calf did not gain weight. On admission to the Ontario Veterinary Hospital, it was given a routine clinical examination and a detailed cardiovascular, Doppler ultrasound examination, which appeared normal. Because of declining health and chronic bloating in the heifer, euthanasia was performed. A detailed post-mortem dissection was carried out. No heart or lung anomalies were detected. Other thoracic organs were normal, except for a helical coil of the esophagus as it traversed the diaphragm. The rumen was located entirely on the right side of the abdomen and had a large area of adhesion to the parietal peritoneal wall. The left kidney was abnormally placed: retroperitoneal and cranial to the right. There were two spleens. The caudal vena cava was distended and twisted to the left. Thus the calf was situs inversus abdominalis. This suggests that the development of laterality in the early embryo may include both timing and positional regulation.     

Multiple congenital malformation in a Holstein calf

A 10-day-old male Holstein dairy calf with orthopaedic abnormalities was unable to stand but was alert with a suckle reflex. At necropsy, the calf showed multiple defects, including partial agenesis of the left rib plate, deformed left scapula, shortened left humerus, agenesis of the left kidney, atresia ani and scoliosis. The cause of these anomalies could not be determined. This report is the first to describe partial agenesis of ribs in a calf.     

Gingival vascular hamartoma in a young Holstein calf

This report describes gingival vascular hamartoma in a 2.5-month-old female Holstein calf. Macroscopically, the mass was 4 × 3 × 1.5 cm in size, 5.5 g in weight, and reddish gray in color. The mass was localized on rostral mandibular gingiva adjacent to the central incisor teeth. Microscopically, the mass was characterized by proliferation of structurally normal blood vessels with all supporting elements.     

Human herpesvirus 8 (HHV-8) detected in two patients with Kaposi's sarcoma-like pyogenic granuloma

AIMS: To report the finding of human herpesvirus 8 (HHV-8) in two patients with Kaposi's sarcoma (KS)-like pyogenic granuloma. This form of pyogenic granuloma closely resembles KS histologically and it has been reported that immunohistochemistry in such lesions may be positive for smooth muscle actin and factor VIII related antigen, which are typically negative in KS. In both patients the lesions were positive for CD31, CD34, smooth muscle actin, and factor VIII related antigen, a profile typical of KS-like pyogenic granuloma. The lesions were tested for the presence of HHV-8 DNA, which to date has been consistently found in all types of KS. METHODS: The lesions were tested for the presence of HHV-8 DNA using the polymerase chain reaction (PCR). A known HHV-8 positive KS specimen was used as the positive control. Six samples of non-KS vascular skin lesions were used as negative controls for the PCR reaction. RESULTS: Both lesions were positive on PCR for HHV-8 and the specificity of product was confirmed by direct sequencing. None of the six control vascular skin lesions was positive for HHV-8. These results strongly indicate KS as the true diagnosis and are supported by the reported clinical course in both cases. CONCLUSIONS: Techniques targeting HHV-8 DNA for detection to confirm a diagnosis of KS are both sensitive and specific. In cases where the differential diagnosis includes KS-like pyogenic granuloma, caution should be taken not to diagnose solely on the basis of immunohistochemistry phenotype. In such cases, PCR targeting HHV-8 DNA sequences is a better diagnostic tool.     

Cleft palate, brachygnathia inferior, and mandibular oligodontia in a Holstein calf

A case of cleft palate, brachygnathia inferior and mandibular oligodontia is described in a calf. Otitis media may be a complication of cleft palate in the calf as it is in man. Cleft palate in the calf may be further classified into at least 4 major groups and some of the cases might be suitable models for a study of cleft palate in man.     

Bilateral cleft lip, jaw, and palate in a female Holstein calf

A female Holstein calf with a body weight of 35?kg was born alive after a normal pregnancy period in September 2010 at a commercial dairy farm in Karaj, Iran. At birth, the cleft lip, jaw, and palate were obvious. The cleft was approximately 8.4?cm in length and 7.2?cm in width at the widest part. Pulse rate, respiration rate, and body temperature of the calf were 112/min, 42/min, and 38°C, respectively. Respiration was abdominal and irregular. Necropsy revealed that there was no more congenital abnormality except for the facial defect. Radiologic examination revealed that the cleft extended from the median lip to the rostral part of the palate, which made the dorsal nose and the palate separate. Based on examination of the craniofacial skeletal in detail following maceration, it was revealed that all the facial bones were developed and the defect was just limited to cleft lip, jaw, and palate, which was resulted from failure of closure in facial processes. Accordingly, abnormality was diagnosed as bilateral cleft lip, jaw, and palate.     

An unusual congenital anomaly in the pulmonary artery and vein of a Holstein calf

This report describes an unusual congenital anomaly found at postmortem in the pulmonary artery and vein of a 4-month-old female Holstein calf with clinical signs of congestive heart failure. Developmental abnormalities found included the pulmonary veins having two branches that opened into the right atrium instead of normal seven to eight, and the pulmonary artery had two branches in addition to the normal left and right branches that enter the lungs. This is the first confirmed case report of a congenital anomaly in the pulmonary artery and vein in the Holstein calf.     

Solitary pulmonary capillary hemangioma: Clinicopathologic and radiologic characteristics of nine surgically resected cases

Background

Solitary pulmonary capillary hemangioma (SPCH) is an extraordinarily rare capillary derived mesenchymal neoplasm. Although routine morphology and immunohistochemistry are adequate for the diagnosis of classical SPCH in surgical specimens, true gross appearance identification of most tumor themselves and diagnosis for some exceptional cases are still very difficult. Furthermore, preoperative imaging and frozen diagnosis remain a challenge.

Lobular carcinoma in situ and lobular cancer (lobular carcinoma) of the breast]

The histological characterization of mammary cancer (MC) is of leading importance for prognosis and choice of therapeutic methods (directed hormonal and chemotherapy). In this respect of interest is the morphological picture of a special form of MC, lobular cancer which is characterized by the most malignant course, marked infiltrative and less manifest destructive growth, multicentricity and multiplicity, as well as bilateral foci of lesions. It probably originates from the myoepithelium. In the national literature, no information on lobular MC is available. Little information is available on the precursor of lobular MC, lobular carcinoma in situ. This problem is insufficiently and inaccurately dealth with in the International classification of mammary gland tumors (WHO). A review of literature on lobular MC, as well as photomicrographs of its structure and that of its precursor are presented.     

食管肉芽肿性毛细血管瘤一例

患者男,58岁.因胃部不适做胃镜检查时,偶然发现食管下段近贲门处有一0.4 cm×0.3 cm大小的暗红色突起,表面稍糜烂,行胃镜下钳取送活检.     

Pathologic changes in murine leishmaniasis (Leishmania donovani) with special reference to the dynamics of granuloma formation in the liver

The histopathologic changes in liver, spleen, and bone marrow of BALB/c mice infected for 6 months with Leishmania donovani are described. Granulomas were the most important histologic lesions found; and the dynamics of their formation, collagen deposition, and resolution in the liver were studied. The number of hepatic granulomas increased until the eighth week and then decreased steadily. In contrast to the liver granulomas, those of spleen and bone marrow do not mature or show collagen deposition. Actual granuloma counts in the liver support the idea that mature granulomas revert to poorly formed ones and finally resolve without scarring. BALB/c mice are suitable for the study of the dynamics of granuloma formation and resolution and the survival of L donovani in an in vivo system. The relevance of these changes to the pathology of L donovani infection in man are discussed.     

Hemangioma of the testis: report of unusual occurrences of cavernous hemangioma in a fetus and capillary hemangioma in an older man

We report two cases of hemangiomata of the testes which occurred in a 17-week-old fetus and a 73-year-old man. To our knowledge, these are the first reported cases of cavernous hemangioma of the testis in a fetus and capillary hemangioma of the testis in an older man. Although a hemangioma of the testis is rare, it should be considered in the differential diagnosis of a testicular tumor. Ann Diagn Pathol 5:80-83, 2001.     

毛细血管瘤组织中间质细胞的免疫组化观察

目的：探讨毛细管瘤组织中间质细胞的作用。方法：采用免疫组织化学Ｓ－Ｐ法观察增殖细胞核怕、内皮细胞抗原、平滑肌肌动蛋白、第Ⅷ因子相关抗原及Ｓ－１００蛋白５种在肿瘤组织中的表达。结果：间质细胞中可见增殖细胞核怕、Ｓ－１００蛋白及平滑肌肌动蛋白的阳性表达染色。结果：毛细血管瘤肿瘤并非均为内皮性，间质细胞可参与肿瘤的组织发生及病理演变。     

Genetic similarities and differences between lobular in situ neoplasia (LN) and invasive lobular carcinoma of the breast

One of the most controversial issues in breast pathology is whether lobular neoplasia (LN) is a risk factor or a precursor lesion of invasive lobular carcinoma (ILC). This is consequent to the fact that no conclusive data on the biology of LN exist. Molecular studies of LN and ILC are scanty, variable, and not consistent. Clonality of 12 cases of LN and ILC present simultaneously in the same block has been studied. Cells from both lesions were obtained by microdissection and were studied for mitochondrial DNA (mtDNA), D-loop sequencing, and neighbor-joining trees. Eight of the same cases were studied with comparative genomic hybridization (CGH) array to have additional data consistent with mtDNA. In all cases, loss of heterozygosity was studied for D16S496,locus 16q22.1 related to e-cadherin. It appears that no fewer than eight cases were genetically very similar (clonal) with mtDNA. Seven of these cases appeared also clonal with CGH array. It is concluded that in the present series, LN and ILC are genetically related lesions in the majority of cases and that LN might be the precursor of ILC.