影响玻片离心沉淀法脑脊液细胞学检查 进一步推广的若干因素

1962年,由原南京医学院侯熙德教授首先引进的新型玻片离心沉淀法脑脊液细胞学(CSFC)检查方法。在粟氏FMMU-5微型(1981年)和孔氏FCS两用型(1993年)细胞玻片离心沉淀器相继研制成功的国产化沉淀器的支持下,经过数十年临床验证,认为该项检查设备简便、易行、投资少、见效快,各级医疗单位在和平或战争条件下均可开展;除有利于患者及时就近就医外,在提高医疗水平、减少不必要的临床误诊和漏诊、降低医疗费用及缓和医     

影响玻片离心沉淀法脑脊液细胞学检查进一步推广的若干因素

1962年,由原南京医学院侯熙德教授首先引进的新型玻片离心沉淀法脑脊液细胞学（CSFC）检查方法,在粟氏FMMU-5微型（1981年）和孔氏FCS两用型（1993年）细胞玻片离心沉淀器相继研制成功的国产化沉淀器的支持下,经过数十年临床验证,认为该项检查设备简便、易行、投资少、见效快,各级医疗单位在和平或战争条件下均可开展;     

脑脊液细胞学检查在诊断中枢神经系统感染性疾病的临床应用

<正>中枢神经系统感染是中枢神经系统(central nervous system,CNS)的常见疾病,脑脊液(cerebrospinal fluid,CSF)检查对疾病的诊断具有重要参考意义[1]。本文采用玻片离心沉淀法检查脑脊液细胞学诊断中枢神经系统感染,现分析如下。1资料与方法1.1病例资料选取2010-03-2013-03间我院收治的中枢神经系统感染患者82例,男43例,女39例,年龄23~67岁,     

中枢神经系统感染性疾病脑脊液检查的临床应用

目前,对于中枢神经系统感染性疾病的诊断仍需依赖脑脊液检查结果,尤其是病原微生物的检查结果。不同炎症反应(包括细菌性、病毒性、肉芽肿性)脑脊液成分特点不同。一般病原微生物培养最具特异性但敏感性较低,实际上,大多数病毒、寄生虫、真菌和慢性细菌性中枢神经系统感染其脑脊液培养常为阴性。血清病原抗体阳性仅能证明体内     

脑脊液细胞学检测临床应用

脑脊液细胞学检测是脑脊液诊断方法的主要项目,也是中枢神经系统疾病的重要辅助诊断方法之一,且对中枢神经系统感染性和非感染性疾病,以及中枢神经系统肿瘤的诊断具有重要意义。免疫细胞化学检测和流式细胞术在脑脊液细胞学中的应用使临床诊断水平提高,普及这一实用且费用低廉的脑脊液诊断技术具有现实意义。     

“脑脊液”液基细胞学检查在中枢神经系统疾病诊断中的应用价值

目的 探讨"脑脊液"液基细胞学在中枢神经系统疾病中的诊断价值.方法 分析41例"脑脊液"液基细胞学检查结果 .结果 液基细胞学检查"脑脊液",根据细胞独特的形态学特点,作出初步诊断.结论 "脑脊液"液基细胞学检查对临床诊断各种疾病有一定的诊断价值,能弥补"脑脊液"常规检查方法 易漏诊获取细胞少的缺陷[1].     

脑脊液细胞学检查在中枢神经系统疾病病因诊断中的价值

在实际的临床工作中我们体会到,应用简便的玻片自然沉淀脑脊液细胞收集法和瑞-姬染色法进行常规脑脊液细胞学检查,对中枢神经系统疾病的病因诊断及鉴别诊断具有重要的实用价值,值得基层医院推广应用。     

中枢神经系统感染105例脑脊液细胞学检查的结果分析

脑脊液细胞学卜sf－C）检查目前已越来越）“泛的应用于临床,。sf－C检查不仅是中枢神经系统感染性疾病的一项极有价值的辅助诊断,而且是评价和判断预后的一项很有意义的实验室技术。本文采用粟化Csf－C检查法对105例中枢神经系统感染患者的脑脊液进行细胞学、常规及生化检查,且的在于研究不同致病原导致的中枢系统感染的脑脊液细胞学变化特点及脑脊液细胞学检查阳性率与常现、生化检查阳性率比较。1研究对象与方法1．1对象本组105例,其中男53例,女52例,年龄3一乃岁,其中化脓性脑炎12例,结脑14例,病毒性脑炎6例,散发性脑炎15例…     

室管膜型原发性中枢神经系统淋巴瘤四例:临床表现及脑脊液细胞学分析

目的分析室管膜型原发性中枢神经系统淋巴瘤(PCNSL)的临床及影像学特点,探讨脑脊液细胞学检测方法在诊断中的价值。方法回顾4例室管膜型PCNSL患者临床诊断与治疗经过,分析其临床特点、影像学改变、脑脊液细胞学和免疫细胞化学染色结果。结果 4例患者平均发病年龄为44岁,发病至就诊时间13 d～5个月。临床表现为头痛、脑膜刺激征,分别伴有脊神经根征(2例)、多组脑神经麻痹(1例)和偏瘫(1例);病程中有间断低热(3例)。其中2例于发病2和4个月时死于脑疝。腰椎穿刺检查脑脊液压力(2例)、白细胞计数(4例)和蛋白定量(4例)升高,葡萄糖降低(3例)。4例患者脑脊液细胞学检测均发现淋巴瘤细胞或异形淋巴细胞,免疫细胞化学染色大多数细胞呈现B细胞标记物阳性。头部MRI增强扫描第三和第四脑室、侧脑室壁异常强化,合并脑室周围强化病灶(2例)和鞍区病变(1例)。PET扫描和骨髓穿刺检查未发现颅外或脊髓受累证据。结论脑脊液细胞学和免疫细胞化学检测是诊断室管膜型PCNSL的重要方法。室管膜及脑室周围病变应考虑PCNSL的可能,尤其MRI增强扫描发现结节样强化者更应提高警惕。     

Detecting infectious agents in central nervous system inflammation by cerebrospinal fluid analysis

Cerebrospinal fluid analysis is the method of choice in CNS infection and provides the basis for appropriate treatment. Due to the proximity of CSF and CNS, the infectious agent may be detected directly by microscopy or antigen or nucleic acid detection--the latter by polymerase chain reaction--in native CSF or after culture. Furthermore, intrathecal antibody synthesis against the infectious agent may identify the cause of infection. This indirect antigen detection method requires correction for a systemic antibody response and a blood-CSF barrier disturbance. The following text gives an overview of appropriate detection methods and their relevance to the most important CNS infections.     

Dosage of lactate in the cerebrospinal fluid in infectious diseases of the central nervous system.

This paper analyzes the diagnosis aid of the dosage of lactate in the cerebrospinal fluid (CSF) in infectious diseases of the central nervous system (CNS). We analyzed prospectively 130 samples of CSF of 116 patients with diagnoses of infectious processes in the CNS. The 130 samples of CSF were divided into five groups: 28 samples of the control group, 40 of bacterial meningitis, 22 of viral meningitis, 16 of fungal meningitis and 24 of patients presenting acquired immune deficiency syndrome (AIDS). The concentration of lactate in the CSF was elevated in the group of patients with bacterial meningitis (average = 46.2 mg/dL), fungal meningitis (average = 27.3 mg/dL) and in the AIDS group (average = 23.5 mg/dL). In the control group and viral meningitis group the lactate content in the CSF presented the reference rates according to the employed method. The lactate dosage in the CSF presented a negative correlation with glycorrhachia and positive correlation with the cellularity and total proteins of the CSF. We conclude that the lactate dosage in the CSF, although unspecific, helps to distinguish the infectious processes of the CNS.     

Diagnosis of a primary non-Hodgkin's lymphoma of the central nervous system by examination of the cerebrospinal fluid

This paper is a report on a case of primary Non-Hodgkin's lymphoma of the central nervous system, diagnosed by cytological and immunological examination of the cerebrospinal fluid. The relevant literature on the subject is reviewed. The importance of examination of the cerebrospinal fluid in cases suspected of this disease is stressed.     

Detection of interferon-gamma and IL-6 producing cells in cerebrospinal fluid cells in the central nervous system infectious diseases using immunocytochemistry]

To evaluate the relationship between cytokines and cerebrospinal fluid (CSF) cells, we detected interferon (IFN)-gamma and interleukin (IL)-6 producing cells in CSF from the patients with central nervous system (CNS) infectious diseases by immunocytochemistry. Five CSF cell smears from three herpes encephalitis patients, three from a patient with EB virus radiculoneuritis, four from the three patients with purulent meningitis, five from five patients with viral meningitis were obtained during early or subacute stages of diseases. Control CSF cell smears were taken from twenty seven patients with motor neuron disease, Parkinson's disease and spinocerebellar degeneration. Immunocytochemistry using specific polyclonal anti-IFN-gamma and IL-6 sera were used to detect each producing cell. Simultaneously, individual positively immuno-reactive cells were morphologically classified macrophage or lymphocyte. The IFN-gamma positive cells immunostained with specific antibody showed brown-colored deposits within the cytoplasm whereas no deposit was in the nucleus (Fig. 1). These phenotype of IFN-gamma positive cells were considered to be lymphocytes or macrophages. However, IFN-gamma-positive macrophages were predominantly seen at the early stages of herpes simplex encephalitis and purulent meningitis. The percent of IFN-gamma positive cells in total CSF cells obtained from the patients with the CNS infectious diseases was 2.3-38.7 as shown in Table 1. The IL-6 positive cells (Fig. 2) were also found early in the course and in subacute stages in the CNS infectious diseases and ranged from 2.5-50 percent in total CSF cells (Table 1). In contrast neither IFN-gamma- nor IL-6-positive cells were detected in non-inflammatory diseases (Table 1).(ABSTRACT TRUNCATED AT 250 WORDS)     

Adenylate kinase activity of cerebrospinal fluid in central nervous system disorders

Adenylate kinase activity was measured in 41 samples of cerebrospinal fluid in 34 patients with various neurological disorders or psychiatric symptomatologies. Activities of the enzyme showed to be linked to clinically estimated acuteness or progression of the changes in the central nervous system at the time of specimen collection. The findings suggest the conclusion that determination of adenylate kinase activity in cerebrospinal fluid is a meaningful tool for the evaluation of progression and/or acuteness of central nervous system disorders.     

脑脊液循环动力学的初步计算机建模分析

目的 应用磁共振相位对比法和计算流体力学软件对脑脊液循环系统进行数值模拟,分析脑脊液循环的动力学特性,并与临床实际相比较.方法 用磁共振相位对比法测定2例正常人的中脑导水管脑脊液流速,并利用临床磁共振扫描图像建市脑脊液循环系统的数值模型,用Matlab软件及Enguage软件提取脑脊液循环系统的轮廓并进行网格划分,然后应用COMSOL Muhiphysics软件进行脑脊液循环系统数值模拟及脑脊液动力学分析.结果 通过模拟软件能模拟脑脊液循环存在的与心动周期相关的有规律的双向流动,表现为心脏收缩期向足侧流动,舒张期向头侧流动,各时相期计算出的速度与我们用磁共振相位对比法测定的在体速度相一致.同时脑脊液循环系统的数值模型能够计算得到颅内压力梯度、脑和脊髓组织固体应力等.结论 脑脊液循环系统的计算机建模分析能无创性得到脑脊液循环的各项动力学量化指标,计算所得与临床实际相符合.本模型为进一步研究一些中枢系统疾病如交通性脑积水、Chiari畸形、脊髓空洞症等的脑脊液动力学机制奠定了基础.