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人工构建特异性互补于HBV基因mRNA SPⅡ增强子区的15-聚反义代脱氧核苷酸并和γ-^32P进行末端标记,以2μmol/L剂量作用于HBV基因转染的HepG2细胞培养24h及48h后检测掺入2.2.15细胞的15-S-asON分别为69.4nmol/L和75.8nmol/L;对HBsAg的抑制率分别为50%和70%;斑点杂交检测HBV DNA,结果表明实验组与对照组与对照组无显著性差异。 相似文献
2.
HBs/CD3双特异性抗体介导LAK对HBVDNA转染细胞的细胞毒作用 总被引:1,自引:0,他引:1
应用化学偶联方法合成HBs/CD3双特异性抗体,并观察其对LAK细胞的细胞毒性作用的影响。用化学偶联剂SPDP将鼠源的CD3与HBs单克隆抗体连接得到HBs/CD3双特异性抗体;设HepG-2和K562细胞对照组。实验显示,HBs/CD3双特异性单克隆抗体能显著提高LAK细胞与2.2.15细胞结合率;应用125IUdR释放试验检测细胞毒性,发现双特异性抗体能增强LAK细胞对2.2.15细胞的细胞毒性,并与抗体浓度相关,BsAb浓度递增,细胞毒性作用随之增加。对细胞毒性作用的特异性研究表明:加入HBs/CD3BsAb后,LAK细胞对2.2.15细胞毒性显著高于HepG-2和K562细胞组。结果表明,HBs/CD3双特异性抗体具有双向识别LAK细胞和2.2.15细胞的特性,特异地促进LAK细胞与靶细胞结合,发挥选择性细胞毒性作用。 相似文献
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目的 观察白细胞介素12真核表达载体(pWRG3169)对HBV-SDNA疫苗(pCR3.1-S)诱导Balb/c小鼠(H-2d)的特异性细胞免疫应答及其对稳定表达HBsAg的小鼠肥大细胞瘤P815细胞(P815-HBV-S)成瘤性的影响。方法 肌肉注射DNA疫苗主白细胞介素12真核表达载体,背部皮下接种P815-HBV-S细胞,观察成瘤情况,4h^51Cr释放法检测小鼠脾细胞CTL活性。结果:对 相似文献
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人工合成两种半乳糖多聚赖氨酸导向配体,与荧光素标记的互补于HBVmRNA多聚腺苷酸信号部分的21-聚反义硫代脱氧核苷酸(ASODN)电性结合,与2.2.15细胞共同孵育,40min后配体组对HBsAg和HBeAg的抑制率为41%~47%和28%~31%,单纯ASODN组为11%和5%,3d后配体组为88%~89%和71%~74%,单纯组为64%和53%,经流式细胞分析,配体组使2.2.15细胞荧光 相似文献
5.
半乳糖修饰的反义硫代寡核苷酸的肝细胞导向及抗乙型肝炎病毒活性研究 总被引:1,自引:0,他引:1
人工合成两种半乳糖多聚赖氨酸导向配体,与荧光素标记的互补于HBVmRNA多聚腺苷酸信号部分的21-聚反义硫代脱氧核苷酸(ASODN)电性结合,与2.2.15细胞共同孵育,40min后配体组对HBsAg和HBeAg的抑制率为41%~47%和28%~31%,单纯ASODN组为11%和5%;3d后配体组为88%~89%和71%~74%,单纯组为64%和53%。经流式细胞分析,配体组使2.2.15细胞荧光染色率达75.3%和83.8%;单纯组为24.3%。结果表明,两种人工配体均具有良好的导向ASODN入肝细胞的功能,且可以明显提高ASODN抗HBV的活性。 相似文献
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应用2.2.15细胞转种裸鼠,建立荷瘤裸鼠HBV动物模型。受种裸鼠皮下可迅速长出移植瘤并分泌HBV多项标志物至外周血液中。应用互补于HBVmRNA SPⅡ增强子区的15聚反义硫代寡核苷酸以20ug/g鼠体重剂量连续治疗10d,结果治疗组荷瘤裸鼠血清中HBsAg、HBeAg及HBV-DNA均受到明显抑制。证明该反义核酸在动物体内同样具有良好的抗病毒效应,为从基因水平研制新一代抗乙肝病毒药物奠定了基础 相似文献
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目前已有许多实验研究证据直接表明Fas/Fas配体(FasL)介导了细胞凋亡。采用免疫组化和原位杂交技术检测HDV/HBV感染树鼠句肝组织中HDAg、Fas/FasL表达;并采用免疫组化双重染色分析病毒抗原表达和Fas/FasL表达之间的关系。1 材料与方法1.1 标本来源 30份HDV/HBV感染树鼠句肝组织,取自本院传染科HDV/HBV感染的树鼠句动物,经ELISA、免疫组化和原位杂交技术证实,HDV在肝细胞中稳定复制、表达,伴ALT升高。1.2 检测方法 ①HDAg、Fas/FasL检测:… 相似文献
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以乙肝病毒(HBV)DNA转染的细胞株2.2.15细胞培养上清液中HBsAg、HBeAg滴度及细胞内HBVDNA中间体的变化作为药物评价指标,应用四甲基偶氮唑盐(MTT)比色分析法测定细胞毒性。结果表明:乳糖化人血清白蛋白单磷酸阿糖腺苷(L-HSA-AraAMP简称交联物)与AraAMP具有相似的抗病毒效应。二者对HBsAg最高抑制率分别为50%,63%,对HBeAs最高抑制率分别为62.6%,67.2%,500μg/ml交联物与100μg/mIAraAMP对HBVDNA有相似的抑制作用。交联物无明显细胞毒性(CD50>2000μg/ml),AraAMP有明显细胞毒性(CD为658.5±180μg/ml),对HBeAg抑制的选择指数分别为>4.8,1.7。展示了受体导向抗病毒药物的良好前景。 相似文献
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10到12岁玻俐维亚男孩的体格测重和肺功能[英1]/Villena,M…//IntJSportsMed.1994Apr,15Suppl2.S75-B.报告23例HAHSES,44例HALSES,43例LAHSES和28例LALSES男孩体格测量结果,... 相似文献
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刘新卷!南京 董文度!南京 徐根兴!南京 樊燕蓉!南京 赵增荣!南京 袁驾南!南京 李侠!南京 凌斌勋!南京 张敏华!南京 刘虹!南京 黄晓兵!南京 裴云!南京 《中华航海医学与高气压医学杂志》1999,(3)
目的 观察高压氧(HBO)对海水淹溺肺水肿(PE-SWD)的预防作用。方法 复制PE-SWD动物模型后,32 只兔随机分为PE-SWD对照组、药物治疗组和HBO组。对3 组兔的动脉血气酸碱指标、Ca2+ 沉淀反应颗粒、c-fos m RNA和c-jun m RNA 等进行自动检测和定量分析比较,并观察分析3组兔的存活时间和海水型呼吸窘迫综合征(seaw ater-respiratory distress syndrom e,SW-RDS)发生率。结果 HBO组兔PaO2 ,SaO2 和pH 3 项指标比药物组显著升高(P< 0.01),动物存活时间[(43.03±7.19)小时]比药物组[(23.58±1.49)小时]明显延长,Ca2+ 沉淀反应颗粒,c-fos m RNA,c-jun m RNA和SW-RDS发生率HBO组则明显低于药物组(P< 0.01)。结论 HBO可明显提高PaO2 和SaO2,有效改善低氧血症和代谢性酸中毒,减轻PE-SWD时肺组织的损伤,从而可防止PE-SWD向SW-RDS转化。 相似文献
11.
Knee injury and Osteoarthritis Outcome Score (KOOS) - validation of a Swedish version 总被引:5,自引:0,他引:5
E. M. Roos H. P. Roos C. Ekdahl L. S. Lohmander 《Scandinavian journal of medicine & science in sports》1998,8(6):439-448
The Knee injury and Osteoarthritis Outcome Score (KOOS) is a self-administered instrument measuring outcome after knee injury at impairment, disability, and handicap level in five subscales. Reliability, validity, and responsiveness of a Swedish version was assessed in 142 patients who underwent arthroscopy because of injury to the menisci, anterior cruciate ligament, or cartilage of the knee. The clinimetric properties were found to be good and comparable to the American version of the KOOS. Comparison to the Short Form-36 and the Lysholm knee scoring scale revealed expected correlations and construct validity. Item by item, symptoms and functional limitations were compared between diagnostic groups. High responsiveness was found three months after arthroscopic partial meniscectomy for all subscales but Activities of Daily Living. 相似文献
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Acute limping may be the result of multiple pathologies in children. The differential diagnosis varies based on the age of the child. Irrespective of age, the initial imaging work-up includes AP and frog leg radiographs of the pelvis and ultrasound; MRI may sometimes be helpful. In children less than 3 years, infections and trauma are most frequent. MRI is the imaging modality of choice when osteomyelitis is clinically suspected. Between the ages of 3 and 10 years, transient synovitis of the hip and Legg-Calvé-Perthes disease are main considerations but infection, inflammation and focal bony lesions are also considered. In children over 10 years, slipped capital femoral epiphysis also is considered. 相似文献
15.
Introduction Ankle sprains are the most common musculoskeletal injury that occurs inathletes particularly in sports that require jumping landing on one foot such assoccer and basketball 《中国运动医学杂志》2008,27(3):378-381
Introduction Ankle sprains are the most common musculo-skeletal injury that occurs in athletes,particularly in sports that require jumping and landing on one foot such as soccer,and basketball(1-4).These injuries often result in significant time loss from participation,long-term disability,and have a major impact on health care costs and resources(5-8). 相似文献
16.
Martin J.Gibala 《中国运动医学杂志》2008,27(3)
KEY POINTS ·High-intensity interval training(HIT)is characterized by repeated sessions of relatively brief,intermittent exercise.often performed with an“a11 out”effort or at an intensity close to that which elicits peak oxygen uptake(i.e.,≥90%of VO2 peak). 相似文献
17.
Objective To investigate endovascular treatment of traumatic direct carotid-cavernous fistulas (CCF) and their complications such as pseudoaneurysms. Methods: Over a five-year period, 22 patients with traumatic direct CCFs were treated endovascularly in our institution. Thirteen patients were treated once with the result of CCF occluded, 8 twice and 1 three times. Treatment modalities included balloon occlusion of the CCF, sacrifice of the ipsilateral internal carotid artery with detachable balloon, coll embolization of the cavernous sinus and secondary pseudoaneurysms, and covered-stem management of the pseudoaneurysms. Results All the direct CCFs were successfully managed endovascularly. Four patients developed a pseudoaneurysm after the occlusion of the CCF with an incidence of pseudoaneurysm formation of 18.2% (4/22). A total number of 8 patients experienced permanent occlusion of the ICA with a rate of ICA occlusion reaching 36.4% (8/22). Followed up through telephone consultation from 6 months to 5 years, all did well with no recurrence of CCF symptoms and signs. Conclusion Traumatic direct CCFs can be successfully managed with endovascular means. The pseudoaneurysms secondary to the occlusion of the CCFs can be occluded with stent-assisted coiling and implantation of covered stents. 相似文献
18.
Tucker VC Hopwood AJ Sprecher CJ McLaren RS Rabbach DR Ensenberger MG Thompson JM Storts DR 《Forensic science international. Genetics》2011,5(5):436-448
In response to the ENFSI and EDNAP groups’ call for new STR multiplexes for Europe, Promega® developed a suite of four new DNA profiling kits. This paper describes the developmental validation study performed on the PowerPlex® ESI 16 (European Standard Investigator 16) and the PowerPlex® ESI 17 Systems. The PowerPlex® ESI 16 System combines the 11 loci compatible with the UK National DNA Database®, contained within the AmpFlSTR® SGM Plus® PCR Amplification Kit, with five additional loci: D2S441, D10S1248, D22S1045, D1S1656 and D12S391. The multiplex was designed to reduce the amplicon size of the loci found in the AmpFlSTR® SGM Plus® kit. This design facilitates increased robustness and amplification success for the loci used in the national DNA databases created in many countries, when analyzing degraded DNA samples. The PowerPlex® ESI 17 System amplifies the same loci as the PowerPlex® ESI 16 System, but with the addition of a primer pair for the SE33 locus. Tests were designed to address the developmental validation guidelines issued by the Scientific Working Group on DNA Analysis Methods (SWGDAM), and those of the DNA Advisory Board (DAB). Samples processed include DNA mixtures, PCR reactions spiked with inhibitors, a sensitivity series, and 306 United Kingdom donor samples to determine concordance with data generated with the AmpFlSTR® SGM Plus® kit. Allele frequencies from 242 white Caucasian samples collected in the United Kingdom are also presented. The PowerPlex® ESI 16 and ESI 17 Systems are robust and sensitive tools, suitable for the analysis of forensic DNA samples. Full profiles were routinely observed with 62.5 pg of a fully heterozygous single source DNA template. This high level of sensitivity was found to impact on mixture analyses, where 54–86% of unique minor contributor alleles were routinely observed in a 1:19 mixture ratio. Improved sensitivity combined with the robustness afforded by smaller amplicons has substantially improved the quantity of data obtained from degraded samples, and the improved chemistry confers exceptional tolerance to high levels of laboratory prepared inhibitors. 相似文献
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LI Yong-dong HAN Xin-wei WU Gang LI Ming-hua 《介入放射学杂志》2007,16(4):253-257
Objective To evaluate the preliminaily clinical efficacy and retrievability of a retrievable hinged covered metallic stent in the treatment of the bronchial stump fistula (BSF). Methods Between April 2003 and March 2005, 8 patients with bronchial stump fistula after pneumonectomy or lobectomy were treated with two types (A and B) of retrievable hinged covered metallic stents. Type A stent was placed in 6 patients and type B in 2 under fluoroscopic guidance. The stent was removed with a retrieval set when BSF was healed or complications occurred. Results Stent placement in the bronchial tree was technically successful in all patients, without procedure-related complications. Immediate closure of the BSF was achieved in all patients after the procedure. Stents were removed from all patients but one. Removal of the stents was difficult in two patients due to tissue hyperplasia. Patients were followed up for 6 - 21 months. Placement of the stents remained stable in all patients except one due to severe cough. Permanent closure of BSF was achieved in 7 (87.5%) of 8 patients. Conclusion Use of a retrievable hinged covered expandable metallic stent is a simple, safe, and effective procedure for closure of the BSF. Retrieval of the stent seems to be feasible. (J Intervent Radiol, 2007, 16: 253-257) 相似文献
20.
Prevalence and severity of hip and groin pain in sub‐elite male football: a cross‐sectional cohort study of 695 players
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K. Thorborg M. S. Rathleff P. Petersen S. Branci P. Hölmich 《Scandinavian journal of medicine & science in sports》2017,27(1):107-114
The purpose of this study was twofold: (a) to investigate the prevalence of hip and groin pain in sub‐elite male adult football in Denmark and (b) to explore the association between prevalence and duration of hip and groin pain in the previous season with the Copenhagen Hip and Groin Outcome Score (HAGOS) in the beginning of the new season. In total 695 respondents from 40 teams (Division 1–4) were included. Players completed in the beginning of the new season (July–Sept 2011) a self‐reported paper questionnaire on hip and/or groin pain during the previous season and HAGOS. In total 49% (95% CI: 45–52%) reported hip and/or groin pain during the previous season. Of these, 31% (95% CI: 26–36%) reported pain for >6 weeks. Players with the longest duration of pain during the previous season had the lowest HAGOS scores, when assessed at the beginning of the new season, P < 0.001. This study documents that half of sub‐elite male adult football players report pain in the hip and/or groin during a football season. The football players with the longest duration of pain in previous season displayed the lowest HAGOS scores in the beginning of the new season. 相似文献