夏至草醇提物对大鼠急性微循环障碍的影响

目的 观察夏至草醇提物对高分子右旋糖酐(Dextran 500)致急性微循环障碍(AMD)大鼠肠系膜血液及淋巴微循环的影响.方法 20只雄性Wistar大鼠被随机分为夏至草组和模型组,每组10只.左颈静脉注射Dextran 500(10 ml/kg)复制AMD模型.6 min后夏至草组自颈静脉缓慢推注夏至草醇提物(6 g/kg),模型组给予等量生理盐水.应用活体显微电视录像系统观察大鼠肠系膜血液及淋巴微循环的变化.结果 与实验前比较,模型组大鼠表现为肠系膜微血管口径缩窄、微血流流速变慢,伴有红细胞聚集,微血流流态积分值显著增高;肠系膜微淋巴管(ML)自主收缩频率、收缩活性指数(Index I)、总收缩活性指数(Index I)、淋巴动力学指数(LD-Index)均显著降低(P均<0.05).注入夏至草醇提物后,大鼠肠系膜微血管口径较模型组扩张,血流加速,微血流流态积分明显降低,ML自主收缩频率、Index Ⅰ、Index Ⅱ、LD-Index显著升高(P均<0.05).结论 夏至草醇提物能明显减轻Dextran 500所致AMD大鼠肠系膜血液及淋巴微循环障碍.     

诃子醇提物对急性血瘀大鼠微观血液流变学的影响

目的通过血液流变学的实验研究,探讨诃子醇提物对大鼠微观血液流变学指标的影响。方法选取SD雄性大鼠随机分为对照组、血瘀模型组、诃子醇提物组,阳性对照组(复方丹参片)。采用皮下注射肾上腺素加冰水冷浴法造大鼠血瘀模型,通过测定全血粘度、血浆粘度,红细胞压积,红细胞在不同切变率下的变形、取向指数,红细胞膜的渗透脆性等血液流变学指标来观察诃子醇提物对大鼠微观血液流变学的影响。结果正常对照组,诃子醇提物组,阳性对照组各血液流变学指标间的差异不明显。但与血瘀模型组比较,诃子醇提物能明显降低全血粘度、血浆粘度、红细胞压积,增强红细胞变形、取向能力,提高红细胞的抗低渗能力。结论诃子醇提物可以有效改善急性应激性血瘀大鼠的微观血液流变学特性。     

夏至草生物碱对大鼠实验性弥散性血管内凝血转归时血流动力学的调节

目的:从微循环、器官血流量和血液流变学角度,探讨夏至草生物碱对弥散性血管内凝血(DIC)的治疗作用.方法:通过静脉注射高分子右旋糖酐Dextran 500复制34只DIC大鼠模型,采用肠系膜微循环、器官微区血流量和血液流变学测定方法,观察夏至草生物碱对DIC转归时微血管口径、器官微区血流量、血黏度及血小板功能的干预作用.结果:大鼠静脉注射Dextran 500后,出现了明显的微循环障碍,表现为微血管收缩、微血流变慢、微血栓形成,且血液流变性异常,表现为血黏度增高、血小板聚集性增强.夏至草生物碱治疗后微血管明显扩张,其流态和微血栓积分值均明显低于生理盐水(NS)对照组(P均<0.05),并能改善胃、肠、肝的微区血流量,效果优于NS对照组(P<0.05),全血黏度、相对黏度、血小板黏附率和聚集率也均明显低于NS对照组,红细胞变形能力则明显增强(P均<0.05).结论:夏至草生物碱通过扩张微血管、增加器官血流量、降低血黏度和抑制血小板聚集,对DIC具有较好的治疗作用.     

西藏高原先心病患者围心脏手术期血液流变学分析

目的探讨西藏高原先心病患者心脏手术期间的血液流变学变化.方法观察高原组(12例)和平原组(12例)患者术前、转流30分、停机后、术后1天、术后3天、术后5天及术后7天的不同切变率下的全血粘度、血浆粘度、全血还原粘度、红细胞压积、红细胞刚性指数及红细胞聚集指数.结果转流期间的各切变率下的全血粘度、血浆粘度均降低(P<0.05),术后1天恢复;红细胞压积、红细胞聚集指数也降低(P<0.05),术后7天才恢复;红细胞刚性指数升高(P<0.05),术后7天恢复.除红细胞刚性指数高原组小于平原组(P<0.05),其他指标高原组均大于平原组(P<0.01).结论高原先心病患者围心脏手术期全血粘度、红细胞聚集性高,红细胞变形性较好,采用控制性稀释技术,有利于微循环灌注.     

四逆汤制剂对血瘀模型大鼠血液流变性的影响

采用全自动锥板粘度计,对因寒致瘀模型大鼠的全血粘度、血浆粘度等血液流变学指标进行了检测,并观察了中药四逆汤对其影响。结果提示：四逆汤能够显著降低血瘀大鼠低切变率下全血粘度,而对血浆粘度无显著影响。证明了民具有温阳化瘀的功效。     

戊酸雌二醇对去势大鼠血液流变学的影响

目的 为探求卵巢切除大鼠在行戊酸雌二醇（一种雌激素）替代治疗后其血液流变学指标的变化。方法 实验动物采用12周正常雌性Wistar大鼠，随机分成假手术组（6只），卵巢切除组（oophorectomized,OOX,10只）和卵巢切除雌激素替代治疗组（estrogen replacement therapy,ERT,10只）。ERT组大鼠在手术4周后，行戊酸雌二醇800mg/Kg/qd替代4周，左心室取血，行血细胞压积，红细胞压积，各切变率下的全血粘度，红细胞变形指数等血液流变学指标的检测，结果 OOX组大鼠的子宫明显出现萎缩，假手术组和ERT组各血液流变学指标间的差异不明显；OOX组低切变率（5s^-1)下的全血粘度值，血浆粘度值，以及红细胞总变形指数和各切变率下的红细胞变形指数均高于假手术组和ERT组；而血细胞压积，高切变率下的全血粘度值和红细胞取向指数三组间的差异不明显。结论 大鼠卵巢切除后行适量的戊酸雌二醇替代治疗，其血液流变学性质可接近于正常鼠水平，而与未行ERT的大鼠存在明显的差异。     

氯沙坦对心绞痛患者心肌缺血的影响

目的　探讨氯沙坦对心绞痛患者血流动力学指标与心肌缺血发作的影响。方法　 4 6例稳定型和不稳定型心绞痛患者随机分为氯沙坦组和对照组 ,测定两组患者血流动力学指标的变化及其对心肌缺血发作的影响。结果　氯沙坦能显著降低心绞痛患者高、低切变率时的全血粘度 ,全血高、低切还原粘度 ;明显改善心绞痛患者红细胞的变形性 ,心绞痛程度显著降低 ,持续时间显著缩短。但对心绞痛患者血浆粘度、红细胞压积、纤维蛋白原、血压、血糖均无明显影响。结论　氯沙坦能改善微循环灌注和心脏供血与供氧 ,有利于预防动脉血栓形成和降低急性心肌梗死的发病率     

几种药物对高原脱适应者血液流变学的影响

目的探讨银杏叶片、复方红景天、复方党参和刺五加片对高原脱适应者血液流变学的影响。方法将驻守海拔5170m 1年的57名健康青年于返回平原前5天随机分为5组,分别口服银杏叶片(12人)、复方红景天(12人)、刺五加 (11人)、复方党参(11人)和安慰剂(11人),返回平原后第7天停药。停约后清晨采空腹静脉血检测全血粘度、全血还原粘度、血浆粘度、红细胞刚性指数、红细胞聚集指数、红细胞变形指数。结果复方红景天组和复方党参组较安慰剂组全血粘度、红细胞压积、全血还原粘度、血浆粘度、红细胞刚性指数、红细胞聚集指数、红细胞变形指数均降低,有显著性意义(P<0．01或0．05),银杏叶片组较安慰剂组全血粘度中切(30s-1)、全血低切还原粘度、血浆粘度降低,有显著性意义(P<0．01或0．05);复方红景天组和复方党参组较刺五加组全血粘度、全血低切还原粘度、血浆粘度、红细胞刚性指数均降低,有显著性意义(P<0．01或0．05)。结论复方红景天、复方党参和银杏叶片三种药物均具有改善组织微循环及血液流变性的作用,使红细胞变形能力增强,血液粘度下降,血流速度增加。且复方红景天和复方党参要优于银杏叶片。     

30例肝硬化患者的血液流变学检测及其临床意义初探

对３０例肝硬化患者的血液流变学参数进行检测，并与健康组对照。结果表明，其全血粘度、血浆粘度、全血还原粘度均有显著升高，红细胞比积减低，变形能力的ＴＫ值增大，血沉加快，体外血栓形成参数无显著差异。说明肝硬化患者总的血液粘度增加，红细胞变形能力减弱，聚集性增强。文中就这些改变的形成机理作了简单探讨。提示在肝硬化患者的综合治疗中，加以改善血液流变学及微循环状态的措施是有一定意义的。     

Binswanger 病血液流变性及血液成份的观察

本文测定了３５例老年Ｂｉｎｓｗａｎｇｅｒ，病患者的部分血液流变学及血液成分的变化．结果发现，与健康对照组和脑动脉硬化组比较，疾病组高切变率全血粘度显著升高，红细胞变形能力明显降低，血液成份Ｈｂ、ＭＣＶ、ＭＣＨ、ＭＣＨＣ、ＲＤＷ显著增加．提示血液成分的老化性改变参与了Ｂｉｎｇｗａｎｇｅｒ病的发生，设法提高提高红细胞变形能力和降低血粘度将有益于阻止该病的发展．     

银杏叶提取物改善学习记忆障碍老龄大鼠痴呆模型的作用途径

背景近年研究显示,银杏叶提取物是一种天然的自由基清除剂,可保护机体免受自由基引起的损害,改善脑循环障碍和神经元的功能,而关于银杏叶提取物对认知功能等高级神经功能活动影响的实验或临床研究相对滞后.目的探讨银杏叶提取物对中枢神经系统高级功能活动的作用,为临床应用银杏叶提取物治疗认知功能障碍提供实验依据.设计随机对照实验.单位华中科技大学同济医学院附属精神病医院老年科,华中科技大学同济医学院病理生理教研室,华中科技大学同济医学院附属协和医院神经内科.材料实验于2002-06在华中科技大学同济医学院基础部完成.选择Wistar大鼠40只,随机分为5组正常老龄对照组、模型组、银杏叶提取物75 mg/kg组、银杏叶提取物150 m/kg组,银杏叶提取物500 mg/kg组,每组8只.方法采用东莨菪碱致老龄大鼠学习记忆障碍的拟老年性痴呆动物模型,其中正常老龄对照组和模型组以相同体积的生理盐水灌胃,银杏叶提取物各组分别按75,150,500 mg/kg银杏叶提取物灌胃,50～400g/次,单次连续给药5 d,于第6天测试水迷宫及避暗试验,测试期间停止灌药,以水迷宫和避暗实验等学习记忆行为训练及生化检测方法,观察用药前后动物学习记忆行为和脑海马区乙酰胆碱及蛋白质含量的变化.主要观察指标①各组大鼠游迷宫所需时间.②各组大鼠游迷宫错误次数.③各组大鼠避暗试验所需时间及错误次数.④各组大鼠脑海马区乙酰胆碱及蛋白质含量.结果银杏叶提取物150 mg/kg组中除1只大鼠不明原因死亡外,银杏叶提取物75,500 mg/kg组各有1只大鼠在灌胃时逃走,最终进入结果分析大鼠为37只.①银杏叶提取物各剂量组造模大鼠游迷宫所需时间和途中错误次数均明显少于模型组(P＜0.05或P＜0.01),模型组大鼠游迷宫所需时间和途中错误次数明显高于正常老龄对照组(P＜0.01).②银杏叶提取物500,150,75 mg/kg组大鼠为达到暗环境被动回避试验内容,学习过程明显短于模型组[(156.78±25.97),(172.66±13.56),(198.54±17.12),(208.34±28.56)s,P＜0.05或P＜0.01].模型组学习时间较正常老年对照组显著延长[(208.46±28.56),(127.46±11.03)s,P＜0.01];银杏叶提取物500,150,75 m/kg组发生电击的错误次数也明显少于模型组[(3.41±0.26),(6.97±0.35),(7.23±0.62),(8.38±0.92)次,P＜0.01],银杏叶提取物500mg/kg组电击次数显著少于150mg/kg组(P＜0.01),150mg/kg组明显少于75mg/kg组(P＜0.05).③银杏叶提取物500,150,75 mg/kg组造模大鼠海马乙酰胆碱含量明显高于模型组[(421.89±36.32),(387.45±32.76),(380.17±41.25),(36528±11.42)mg/g,P＜0.05或P＜0.01],而银杏叶提取物500 mg/kg组的海马乙酰胆碱含量显著高于银杏叶提取物150,75 mg/kg组(P＜0.01).另外,与正常老龄对照组比较,东莨菪碱致学习记忆障碍模型组大鼠海马脑区的蛋白质含量显著降低[(41.75±3.82),(95.13±6.34)mg/kg,P＜0.01],在给予银杏叶提取物后,造模后的实验大鼠海马脑区蛋白质含量虽有所增加,但差异无显著性意义(P＞0.05).结论银杏叶提取物呈剂量依赖性显著改善实验动物的学习记忆能力,并显著增加海马脑区乙酰胆碱含量.     

Nonresuscitated endotoxemia induces microcirculatory hypoxic areas in the renal cortex in the rat

The pathophysiology of acute renal failure (ARF) in sepsis is only partly understood. In several animal models of septic ARF, no profound tissue hypoxia or decrease in microcirculatory PO2 (microPO2) can be seen. We hypothesized that heterogeneity of microcirculatory oxygen supply to demand in the kidney is obscured when looking at the average microPO2 during endotoxemia. In 20 anesthetized and ventilated rats, MAP, renal blood flow (RBF), and creatinine clearance (CLcrea) were recorded. Renal microPO2 was measured by phosphorescence quenching, allowing measurement of microPO2 distributions. Five animals received a 1-h LPS infusion (10 mg kg h). In 5 rats, RBF was mechanically reduced to 2.1 +/- 0.2 mL min. Five animals served as time control. LPS infusion significantly reduced RBF to 2.1 +/- 0.2 mL min and induced anuria. Average cortical microPO2 decreased from 68 +/- 4 to 52 +/- 6 mmHg, with a significant left shift in the cortical oxygen histogram toward hypoxia. This shift could not be observed in animals receiving mechanical RBF reduction. In these animals, CLcrea was reduced to 50%. An additional group of rats (n = 5) received fluid resuscitation. In these animals, RBF was restored to baseline, CLcrea increased approximately 50%, and the cortical microcirculatory hypoxic areas disappeared after resuscitation. In conclusion, endotoxemia was associated with the occurrence of cortical microcirculatory hypoxic areas that are not detected in the average PO2 measurement, proving the hypothesis of our study. These observations suggest the involvement of hypoxia in the pathogenesis of endotoxemia-induced ARF.     

动静脉脉冲泵对危重患者上肢静脉回流障碍作用的临床研究

目的观察动静脉脉冲泵(artery vein impulse pump,A-V脉冲泵)对危重患者上肢静脉回流障碍作用及手功能的影响。方法将60例ICU非创伤性手肿胀患者随机分为对照组和观察组各30例。对照组采用常规抬高患肢方法消肿,观察组采用A-V脉冲泵治疗。治疗前后对2组患者患手的肿胀程度以及掌指关节活动度进行评定与比较。结果治疗后第7天及第14天,2组患者患手肿胀程度差异均有统计学意义(P0.05)。治疗后2组患者患手掌指关节活动度差异有统计学意义(P0.05),观察组患手肿胀程度恢复和患手掌指关节活动度均较对照组好,且观察组较治疗前明显好转(P0.05),对照组好转不明显(P0.05)。结论 A-V脉冲泵能显著改善危重患者手肿胀和手掌指关节活动度。     

Coagulation-physiological studies on the prevention of thromboembolism with dextran 60. I. The effect on platelet function

Platelet aggregation, spreading capacity, adhesiveness (methods according to BREDDIN) have been investigated in 3 groups of 20 patients each after gynecological surgery. Group A (control group) got only 4-hydroxycumarin (Sintrom), group B--in addition to that--intraoperative 500 ml Dextran 60 (Macrodex 6%), group C 500 ml Dextran 60 as well intraoperative as on the 1., 2., 4., 7. and 10. day after surgery. The present data show that depending on the duration of Dextran application, platelet spreading capacity and adhesiveness are inhibited differently as to duration and extent. There was, above all, a reduction and with that a normalisation of the postoperatively increased spontaneous platelet aggregation. It is possible to influence the phase of early postoperative embolism in a favorable way by applicating Dextran already intraoperatively. After that one can use further infusions of Dextran, a prophylaxis by Cumarins or other anticoagulants. Giving dextran only once during surgery one can prove this normalisation of the increased platelet aggregation up to 24 hours postoperatively. By further infusions of Dextran 60 on the 1., 2., 4., 7. and 10. postoperative day it is possible to keep the extent of aggregation on a significantly reduced level till the complete mobilisation of the patient and consequently attain a protective effect on a possible development of deep venous thrombosis of the legs.     

中性粒细胞与淋巴细胞比值对ST段抬高型心肌梗死患者直接经皮冠状动脉介入治疗后微循环障碍的预测价值

目的基于微循环阻力指数（IMR）探讨ST段抬高型心肌梗死（STEMI）患者中性粒细胞与淋巴细胞比值（NLR）和直接经皮冠状动脉介入治疗（PCI）术后微循环障碍的关系。 方法选择2016年1月至2018年12月期间苏北人民医院收治的50例急性STEMI患者为研究对象,将患者分为低NLR组（NLR ≤ 6.17,25例）和高NLR组（NLR > 6.17,25例）。比较两组患者的临床资料、冠状动脉病变情况、介入治疗方案及直接PCI术后IMR值,并采用Pearson相关分析NLR与直接PCI术后IMR的相关性。 结果低NLR组和高NLR组患者糖尿病病史（7/25 vs. 15/25,χ² = 5.195,P = 0.023）、高血脂病史（7/25 vs. 14/25,χ² = 4.023,P = 0.045）、中性粒细胞计数[6.33（4.04,6.66）× 10⁹/L vs. 7.90（7.23,12.09）× 10⁹/L,H = 18 716.000,P < 0.001]、淋巴细胞计数[1.85（1.37,2.56）× 10⁹/L vs. 0.87（0.78,1.03）× 10⁹/L,H = 29 710.000,P < 0.001]、发病-球囊扩张时间[227.00（180.00,390.00）min vs. 360.00（270.00,435.00）,H = 25 199.500,P < 0.001]及直接PCI术后IMR值[（29.9 ± 2.7）vs.（40.4 ± 1.7）,t = 16.307,P < 0.001]比较,差异均有统计学意义。Pearson相关分析显示,NLR与IMR具有正相关性（r = 0.676,P < 0.001）。进一步简单线性回归分析示,患者NLR与直接PCI术后微循环障碍的发生有关（R² = 0.457,P < 0.001）。 结论STEMI患者入院时,较高的NLR水平与直接PCI术后严重的微循环障碍密切相关,对预测STEMI患者直接PCI术后微循环状态有一定的参考价值。     

Coagulation-physiological studies on the prevention of thromboembolism with dextran 60. II. Changes in coagulation and fibrinolysis

The postoperative behavior of different parameters of coagulation and fibrinolysis have been studied in three groups of 20 patients each. Group A got only 4-hydroxycumarin, group B 500 ml Dextran 60 intraoperatively in addition to that, group C 500 ml Dextran 60 during surgery and on the 1., 2., 4., 7. and 10. postoperative day. The results show that the hypercoagulability which occurs immediately after surgery can be prevented by infusion of Dextran 60. On the other hand there is no evidence that Dextran 60 may cause a consumptive coagulability. There is only little influence on fibrinolysis, so that no defect of haemostasis can be expected. So it is possible to use Dextran 60 both for bridging over the phase of early embolism after surgery until the change to other anticoagulants and as a substance which can be applicated for a longer period of time in prophylaxis of thromboembolism. It is referred to the additional properties of Dextran 60 in prevention of thrombosis due to reduction of blood viscosity and improvement of microcirculation.     

实验性弥散性血管内凝血大鼠淋巴循环的变化

目的 探讨实验性弥散性血管内凝血（DIC）大鼠淋巴循环的变化。方法 32只雄性Wistar大鼠按随机数字表法分为DIC组（n=16）和假手术组（n=16），每组中8只动物用于肠系膜淋巴微循环观察，另8只进行淋巴动力学检测。颈静脉注射高分子右旋糖酐（dextran 500）制备Wistar大鼠实验性DIC模型，通过淋巴学方法，观察DIC时大鼠淋巴循环的变化。结果DIC时，肠系膜微淋巴管（ML）收缩性、肠淋巴流量、淋巴细胞输出量明显降低，淋巴液中有少量单核细胞，并且淋巴液黏度较高。经生理盐水治疗后，ML收缩性、肠淋巴流量、淋巴细胞输出量均显著升高，淋巴液中有大量单核细胞出现，与假手术组比较差异均有显著性（P均〈0．05），且淋巴液黏度明显低于DIC组（P〈0．05）。结论 Wistar大鼠实验性DIC时，淋巴循环障碍表现为ML收缩性降低、淋巴循环转运功能障碍和淋巴液黏度增高。     

Effects of eritoran tetrasodium, a toll-like receptor 4 antagonist, on intestinal microcirculation in endotoxemic rats

Lipopolysaccharide (LPS) or endotoxin can induce Toll-like receptor 4 signaling and cause microcirculatory dysfunction, which can lead to multiple organ dysfunction. The goal of this study was to investigate whether Toll-like receptor 4 antagonist, eritoran tetrasodium, can attenuate microcirculatory dysfunction in endotoxemic rats. Seventy-two male Wistar rats were divided into three groups as follows: control, LPS, and eritoran + LPS. These rats received laparotomy to exteriorize a segment of terminal ileum for microcirculation examination on intestinal mucosa, muscle, and Peyer patch. The rats in the eritoran + LPS group received 10 mg kg?1 eritoran intravenously. The rats in the LPS and eritoran + LPS groups received 15 mg kg?1 LPS intravenously. Microcirculatory blood flow intensity was measured by full-field laser perfusion imager. Total and perfused small-vessel densities, microvascular flow index, and heterogeneity index were investigated by sidestream dark-field video microscope. Our results revealed that eritoran restored the mean arterial pressure. At 240 min, the microcirculatory blood flow intensity was higher in the eritoran + LPS group than in the LPS group as follows: mucosa (1,094 [SD, 398] vs. 543 [SD, 163] perfusion unit [PU]; P < 0.001), muscle (752 [SD, 124] vs. 357 [SD, 208] PU; P < 0.001), and Peyer patch (961 [SD, 162] vs. 480 [SD, 201] PU; P < 0.001). Eritoran also attenuated endotoxin-induced elevation in the serum level of D-dimer. In conclusion, we have established a promising rat protocol to investigate the intestinal microcirculation in endotoxemia. Our data indicate that eritoran can reduce microcirculatory dysfunction in endotoxemic rats.     

强化胰岛素治疗对脓毒症大鼠肠道微循环的影响研究

目的 探讨强化胰岛素治疗对脓毒症时小肠微循环障碍的影响.方法 采用盲肠结扎穿孔术(CLP)制备脓毒症模型.将雄性SD大鼠随机分为对照组、脓毒症组、胰岛素组,每组16只.于给药后3、6、12、24 h各处死4只大鼠,取肠组织,采用放射免疫法检测血小板活化因子(PAF)、前列环素(PGI2)和血栓素A2(TXA2)含量.结果 脓毒症组大鼠制模后3 h PAF、PGI2、TXA2水平即明显升高,6 h达峰值,之后逐渐下降;不同时间点均较对照组明显升高(P均<0.05),且以TXA2升高明显,PGI2/TXA2比值降低.胰岛素组各时间点PAF、PGI2、TXA2水平均较脓毒症组明显降低,以TXA2降低较明显(P均<0.05),PGI2/TXA2比值升高.结论 胰岛素可通过改善PGI2/TXA2系统的失衡,对抗PAF过度分泌而减轻肠道微循环血管痉挛、抗血小板聚集、抑制血栓形成,从而起到保护脓毒症时肠道功能的作用.     

Effects of cimetidine, a histamine type 2 receptor antagonist, on microcirculatory disturbance of the stomach induced by full-thickness burn injuries as a model for surgical stress in rats

Background

We have reported the preventive effect of cimetidine, a histamine type 2 receptor antagonist, on decreased gastric mucosal blood flow induced by burn injury, a model of surgical stress.

Objective

The present study was performed to assess the effects of cimetidine on gastric microcirculatory disturbance induced by surgical stress.

Methods

Twelve male Wistar rats were anesthetized and a 30% full-thickness dorsal scald burn was inflicted. The total lengths of gastric erosions were measured using stereoscopic microscopy. Microvascular images in the basal region of the gastric mucosa were observed using an intravital microscope, and the diameters of venules and collecting venules were measured. Rolling leukocytes inside and along the venules, an indication of endothelial damage to the microcirculation, were observed. The rats were assigned to the cimetidine group or the control group (both, n = 6). Cimetidine 100 mg/kg was administered 30 minutes before and 2.5 hours after infliction of the burn injury. Animals in the control group were given only isotonic saline. Values are expressed as mean (SD).

Results

Contraction of venules was easily observed in all rats in the control group. However, venular contraction was rarely observed in the cimetidine group. The total length of gastric erosions was significantly decreased in the cimetidine group compared with the control group (0.93 [0.58] μm vs 5.98 [5.18] μm, respectively; P < 0.05). The percentages of rolling leukocytes that passed the confluence of a prevenule and a venule were also significantly decreased in the cimetidine group compared with the control group (4.7% [7.3%] vs 22.6% [5.7%]; P < 0.01). The diameters of the venules and collecting venules were significantly larger in the cimetidine group (57.3 [6.8] μm and 75.9 [3.6] μm, respectively) than in the control group (30.9 [9.2] μm and 46.8 [8.0] μm, respectively) (both, P < 0.01).

Conclusions

The present study suggests that cimetidine may have a protective effect against gastric microcirculatory disturbances induced by burn injury, a model of surgical stress.