4镜头聚焦职场″白骨精″

听过这样一句话:女人,工作着是美丽的。言外之意是说,女人们只有在工作中实现了自身的价值后,才会更加有魅力。不错,市场竞争激烈而残酷的二十一世纪,在职场上与男人并肩作战,同样能统领上百人的团队工作的女人通常被戏称为″白骨精″(白领、骨干、精英)。为了修炼成″白骨精″,女人们往往要透支美丽与健康———事业与家庭的双重重负在心中投下挥之不去的暗影。她们所承受的心理压力理应引起自身乃至整个社会的关注。所以,本期我们将就这个话题与大家谈谈在心理健康门诊中常见的职业女性心理暗疾,希望工作中的女人们永远携美丽与健康同行。     

终止″功血″中医为上

人间四月,春光潋滟,功能性子宫出血却往往好发于这个美好季节,北京益康堂专家提醒女性朋友珍视健康,尽享春日的无限风情。     

纳米空气收伏″无形杀手″

这个″杀手″可不是影视作品中出现的那些亡命之徒,他们似乎离我们的生活很遥远。虽然我们看不到这些隐形的″杀手″,但它们却是无处不在的。它们的家族由大肠杆菌、绿脓杆菌、金黄色葡萄球菌、链球菌以及甲醛、苯、VOC等等许多人们甚至叫不上名字的家伙所构成。家居中、爱车里、办公室内……许多让我们意想不到的地方,它们无孔不入地掠夺着人类的健康,而我们却往往忽视″杀手″的存在。     

″健康折纸明星″浓情招募中

折纸唤起了人们童年的纯美回忆,不仅如此,折纸更折射出了许多人内心深处健康美好的热望。在新一年的春风又绿江南岸之际,让我们的手指在彩色的纸张上跳舞,让精彩的创意折出你的梦想和对心中挚爱的祝福和期盼。来吧!亲爱的读者,《ModernHealth》读者俱乐部的真正主人们,无论海角天涯,让我们在春天里赴一场手指运动的约会,让精美灵动的折纸作品连接你我他的心愿!快快行动吧,幸运的″健康折纸″明星也许就是你!     

多了″第三者″床第也逍遥

热恋中的爱如潮水和新婚中的如胶似漆很平常,难得的是朝朝暮暮的激情男欢女爱。特别是夫妻之间出现了″第三者″———孩子以后,新任母亲的身体和情绪开始逐渐演变成类似家务劳动的厌倦。更有甚者,性冷淡、性厌恶、性恐惧……情同陌路。那么,如何使夫妻性爱在可爱的″第三者″出现后依旧″性″福美满,希望下面几个故事对你有所帮助。     

Expression and clinical significance of EGFR, PCNA, Bcl-2 and Bax in thymoma

Objective:To study the expressions and clinical significances of PCNA, EGFR, Bcl-2 and Bax in thymoma. Methods: The expressions of EGFR, PCNA, Bcl-2 and Bax in 46 cases of thymoma and 11 cases of normal thymus were detected with S-P immunohistochemistry. The results were analyzed with the pathologic indexes. Results: The positive rates of EGFR, Bcl-2 and Bax in normal thymus were 18. 2%, 9. 1%,and 18.2% respectively, while in thymoma were 71.7%, 41.3%, and 15.2% separately. The expression of EGFR in thymoma was significantly correlated with Masaoka staging and Levine classification. The survival rate of EGFR negative patients was significantly higher thanthat of EGFR positive patients (P＜0. 01). PCNA labelling index was significantly higher in thymoma as (4.00±1.87)% than in normal thymus, (2.68±0. 62)% , which was significantly correlated with Levine classification. The expression of Bcl-2 in thymoma was also significantly correlated with Levine classification. The survival rate of Bcl-2 negative patients was significantly higher than that of Bcl-2 positive patients (P＜0. 01). The expression of PCNA, EGFR, Bcl-2and Bax in thymoma had no correlation with histologic type and myasthenia gravis (MG) (P＞0. 05). Conclusion: The expression of EGFR may be involved in the occurrence and development of thymoma. EGFR can be regarded as a supplementary predictor for Masaoka staging so as to predict the progression accurately.The expression of Bcl-2 may contribute to the occurrence of thymic carcinoma and Bcl-2 can be used as a biomarker identifying thymic carcinoma.     

长瓣金莲花中牡荆素2″—木糖甙和荭草素2″—木糖甙的分离和结构测定

从长瓣金链花TrolliusmacropelalusFr.Schmidt的干燥花中首次分得2个单体化合物，根据理化性质和光谱（IR、MS、1HNMR、13CNMR中PND和DEPT）解析，分别鉴定为牡荆素2″－O-β-D-吡喃木糖甙（Ⅰ）和荭草素2″－O-β-D-吡喃木糖甙。     

Methylation of RAR-β2, RASSF1A, and CDKN2A Genes Induced by Nickel Subsulfide and Nickel-carcinogenesis in Rats

Objective To investigate the expression variation of RAR‐β2, RASSF1A, and CDKN2A gene in the process of nickel‐induced carcinogenesis. Methods Nickel subsulfide (Ni 3 S 2 ) at dose of 10 mg was given to Wistar rats by intramuscular injection. The mRNA expression of the three genes in induced tumors and their lung metastasis were examined by Real‐time PCR. The methylation status of the 5’ region of these genes were detected by Quantitative Real‐time methylation specific PCR. Results The mRNA expressions of t...     

Expression and Significance of Bcl-2, Bax, Fas and Caspase-3 in Different Phases of Human Hemangioma

Summary： The relationship between Bcl-2, Bax, Fas, caspase-3 and development of hemangioma and the molecular mechanism was investigated. By using immunohistochemical S-P method, proliferating cell nuclear antigen was detected. According to the classification of Mulliken in combination with PCNA expression, 27 cases were identified as proliferating hemangioma and 22 cases as involutive hemangioma. Five normal skin tissues around the tumor tissue served as controls. By using immunohistochemical technique, the expression of Bcl-2, Bax, Fax and Caspase-3 was detected. The cells expressing Bcl-2, Bax, Fax and cappase-3 were identified as hemangioma endothelia by immunohistochemical staining of Ⅷ factor. The average absorbance （A） and average positive area rate of Bcl-2, Bax, Fas and caspase-3 expression were measured by using HPIAS-2000 imaging analysis system. The results showed that the expression of Bcl-2 in the endothelia of proliferating hemangioma was significantly higher that in involutive degenerative hemangioma endothelia and vascular endothelia of normal skin tissue （P〈0.01）. The expression of Bax, Fas and Caspase-3 in the endothelia of involutive hemangioma was obviously higher than in the endothelia of proliferating hemangioma and normal skin tissue （P〈0.01）. The expression of BAx and Fas in endothelia of proliferating hemangioma was higher than in those of normal skin tissue （P〈0.05）. It was suggested that Bcl-2, Bax, Fas and caspase-3 might be involved in the development and involution of hemangioma. Bcl-2 could promote the growth of hemangioma by inhibiting apoptosis of endothelia. Bax, Fas and caspase-3 promote the switch of hemangioma from proliferation to involution by inducing the apoptosis of hemangioma endothelia.     

Job Stress, Gene Polymorphism of β2-AR, and Prevalence of Hypertension

Objective To study the interactive effect of job stress and genetic susceptibility （or gene polymorphism） on hypertension. Methods A cross-sectional epidemiological study was conducted in 452 workers from a thermal power plant in China. Extrinsic effort, occupational reward, and over-commitment were measured. Hypertensive patients were defined by three phases of screening, reexamination, and final diagnosis. β2-AR genotypes and allele frequencies at amino acid positions 16 （β2-AR-16： Arg→Gly） and 27 （β2-AR-27： Gln→Glu） were identified by PCR-RFLE Results Job stress was related with the prevalence of hypertension in males （P〈0.05）, whereas no significant relationship was found in females （P〉0.05）. Differences in genotypes and allele frequencies of the β2-AR-16 were statistically significant between the hypertension and control groups （P〈0.05）, whereas those of β2-AR-27 were not （P〉0.05）. The prevalence of hypertension was higher in individuals carrying Gly16 allele than in those carrying Arg16 allele of the high job stress group （P〈0.01 or 0.05）. Conclusion High job stress and polymorphism of β2-AR-16 have an interactive effect on the prevalence of hypertension in male workers.     

Expression and significance of EGFR,C-erbB_2 and PCNA in primary breast carcinoma tissues

Expression and significance of EGFR,C-erbB_2 and PCNA in primary breast carcinoma tissues@孙治君$Dept Gene Surg,1st Affil Hosp Chongqing Med Univ,Chongqing 400016     

Expression of ER, PR, P53, HER-2 and TOP Ⅱ in 120 cases with breast cancer and it's clinical significance

目的:研究l20例乳腺癌中ER、PR、P53、HER-2及TOPⅡ表达和临床意义.方法:应用免疫组化SP法测得ER、PR、P53、H ER-2及TOPⅡ在120例乳腺癌中表达情况.结果:ER、PR、P53、HER-2及TOPⅡ的表达分别是:48(40.00％)、50(42.67％)、71(59.17％)、67(55.83％)、69(57.50％).P53的表达与HER-2呈正相关,1P53、HER-2的表达与ER,PR呈负相关(P ＜0.05);ER、PR的表达与肿瘤临床分期及淋巴结转移呈负相关.P53、HER-2的表达与肿瘤临床分期及淋巴结转移呈正相关(P＜0.05).结论:TOPⅡ、HER-2是判断乳腺癌预后的参考指标,ER、PR、P53、HER-2及TOPⅡ联合检测对乳腺癌治疗方案及预后判断有重要临床意义.     

Identification of two new mu-adaptin-related proteins,mu-ARP1 and mu-ARP2

Ｉｄｅｎｔｉｆｉｃａｔｉｏｎｏｆｔｗｏｎｅｗｍｕａｄａｐｔｉｎｒｅｌａｔｅｄｐｒｏｔｅｉｎｓ，ｍｕＡＲＰ１ａｎｄｍｕＡＲＰ２ＸｉａｏｌｕＷａｎｇａｎｄＭａｎｆｒｅｄＷ．ＫｉｌｉｍａｎｎＷｅｒｅｐｏｒｔｔｈｅｃＤＮＡｃｌｏｎｉｎｇ，ｐｒｉｍａｒ...     

Effects of Cimetidine, H_2 Receptor Antagonist,on Follicular and Luteal Developmentin the Mice

INTRODUCTIONHistaminehasbeenimplicatedinseveralphysiologicalfunctionsintheovarysuchasLH-inducedincreaseinovarianbloodflow,oedemaformation,ovulationandfollicularandlutealdevelopment(Krishnaetal.,l989).Histamineparticipatesinthemechanismofovulationmainlythr…     

P53 Gene Mutation and Expression of MDM2, P53, P16 Protein and their Relationship in Human Glioma

To investigate the effect of P53 protein accumulation and p53 gene mutation in the pathogenesis of glioma and to study the role of MDM2, P53 and P16 protein in glioma formation and progression and their relationship with each other, LSAB immunohistochemical staining method and non-isotopic PCR-SSCP techniques were used to detect the expression of MDM2, P53 and P16 protein and p53 gene mutation in 48 cases of gliomas. The results showed that the positive expression rate of MDM2, P53 and the negative rate of P16 was 22.9 %, 41.7 % and 60.4 %, respectively. The latter two in high grade （grade Ⅲ , Ⅳ） gliomas had a significantly higher rate than in the low grade （grade Ⅱ ） gliomas. Moreover, the co-expression of MDM2 and P53 protein was confirmed in only 1 of 48 cases. No significant difference was found in the rate of the expression of MDM2 between high grade and low grade gliomas （P〉0.1） . PCR-SSCP results showed that mutation of 5 --8 exons of p53 gene was detected in 17 out of 48 cases （35.42 %） . Mutation was detected in 16 of 20 cases of positive p53 expression, and another one was detected in 28 cases of negative expression cases. The correlation between p53 mutation and p53 immunopositivity was observed in 89.6 % of the cases. P53 gene mutation and the level of MDM2, P53 and PI6 protein were not related to age, gender of the patients, tumor location and size. It is concluded that the mutation of p53 and deletion of p16 might play important roles in the tumorigenesis of gliomas and it was significantly associated with the grade of tumor differentiation. P53 protein accumulation can indirectly reflect p53 mutation. MDM2 amplification and overexpression might be an early event in the growth of human gliomas.     

Oncogenes, Oncogenesis, and Oxygen Radicals

The role that free radicals in general and oxygen radicals in particular play in carcinogenesis has attracted considerable attention in recent years. The oxygen radicals are undesirable but inevitable products of aerobic metabolism in the normal living cell. The cellular antioxidant defense system maintains an appropriate balance between necessary oxidative events and those that are excessive. When this critical balance cannot be maintained because of the overloading of the cellularredox system, oxygen radicals can induce cell damage. They can influence carcinogenesis by inducing DN A damage from direct oxidation or indirectly from DNA-binding products of lipid peroxidation. Oxygen radicals can induce conformational changes in the plasma membrane by lipid peroxidation and protein degradation, thus influencing membrane-associated cellular activities. They are capable of affecting membrane-bound protein kinases, growth factors and their receptors, and, therefore, signal transduction and oncogene activation. Thus, the oxygen radicals can have a major influence on oncogenes and oncogenesis. (C)1990 Academic Press.Inc.     

Embryotoxicity and Teratogenicity of Cu~(2+) and Zn~(2+) for Xenopus Laevis, Assayed by the FETAX Procedure

Cupric chloride (CuCl_2) and zinc chloride (ZnCl_2) were tested by the FETAX (frog embryo teratogenesis assay: xenopus) procedure.Results: In control embryos, the survival rate at 101h post-fertilization was>95%) and teratogenic rate was>7%. The teratogenic concentrations (EC_(10), EC_(50), EC_(90)) of Cu~(2+) were 0.9, 2.5 and 4.4μmol/L; the embryolethal concentrations(LC_(10), LC_(50), LC_(90)) of Cu~(2+) were 10, 22 and 40 μmol/L; the teratogenic index(TI=LC_(50)/EC_(50))for Cu~(2+) was 8.8. EC_(10),EC_(50),EC_(90) of Zn~(2+) were 6.7,40 and 70μmol/L: LC_(10), LC_(50), LC_(90) of Zn~(2+) were 410, 850 and 1080μmol/L, TI for Zn~(2+) was 21. TI(1.5) was considered the threshold for positive teratogenicity in the FETAX assay. Both metal ions were shown to be potential teratogens for xenopus(TI>1.5). Mortality and teratogenic rate in embryos exposed to Cu~(2+) and Zn~(2+) were shown concentration-response relation. Head-to tail lengths of the tadpoles, measured at 101h post-fertilization were negative correlation with the Cu~(2+) and Zn~(2+) concentrations (r=0.95). The minimum concentration to inhibit growth,(MCIG) was 10 μmol/ L for Cu~(2+) and 300 μmol/L for Zn~(2+).The anomalies observed in Cu~(2+) and Zn~(2+) exposed tadpoles primarily affected the face、eyes、heart、gut、notochord and fins. The most common facial anomaly was a beak-like deformity of the snout, eye malformations included incomplete closure of the choroid fissure, herniation of the selera. Cardioproptosis was frequent findings, hypertrophy and distention of the gut were commonly noted. Flexion was frequent at the tip of the notochord or at the midsection of the notochord, associated with broad, ruffled fins. The authors emphasize that caution and scientific judgment must be used in extrapolating the results of FETAX assays to predict teratogenic risks for man and other mammals.