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1.
外源性谷胱甘肽在大鼠的药代动力学和器官组织分布 总被引:10,自引:1,他引:9
研究外源给予GSH的药动学特性及其是否被器官组织利用。采用反相C18-ODS柱,荧光检测器,经丹磺酰氯衍化反应后分离和测定大鼠血浆及器官组织GSH水平。结果:静注200mg/kg,腹腔注射600mg/kg和1200mg/kgGSH给大鼠后,血药浓度-时间曲线均反映GSH大鼠体内过程符合二室模型,其T1/2α分别为0.06h(IV),0.75h(IP)和0.22h(IP),T1/2β依次为0.54、 相似文献
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目的 主要研究谷胱甘肽(GSH)口服后的吸收情况。方法 Beagle犬5只,先后分别经口、静注给予GSH及其构成氨基酸后,用HPLC法测定血中浓度。结果 在清醒犬,单剂量(3.89、1.62mmol/kg)一次或单剂量(0.49mmol/kg)连续5、10d经口给GSH后,犬前肢静脉血浆中GSH、甘氨酸明显升高(P<0.05~0.01);如上单次给予1.62mmol/kg(等于GSH分子中含有的摩尔数)构成氨基酸后,血浆中的GSH水平未见变化,但构成氨基酸均显著增加(P<0.01);在麻醉犬,经口给GSH1.62mmol/kg后,1~6h内肠系膜静脉血浆GSH水平高于前肢静脉(P<0.05~0.01);清醒犬ivGSH0.065mmol/kg后静脉血浆中GSH的Kel1和T1/2α分别为8.04h-1和0.09h,且在降至基值后又出现一个再分布的小峰。结论 GSH经口给药后部分可以整分子吸收,经肝脏后血浆中GSH浓度明显下降,可能是由于肝脏的摄取所致。 相似文献
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以尼群地平为内标,用反相高效液相色谱法(RP-HPLC)测定兔口服地尔硫Zhuo后血浆和组织器官的药物浓度。药动学符合二房室模型。主要参数:T1/2(a)=0.51h,T1/2(β)=6.11h,T1/1/(ka)=0.25h,T(p)=0.52h,口服2h后在胆汁、血、脾、心、肾、肝、大脑和小脑药物浓度分别为:0.559mg/L、0.375mg/L、4.562μg/g、0.771μg/g、1.0 相似文献
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研究苯酚类螯合剂8102在大鼠体内的药代动力学方法采用液体闪烁计数技术测量^3H-8102在血液、组织、尿和粪中的放射性强度。结果大鼠肌注8102(20、50、100mg/kg)后,其体内血药浓度-时间曲线符合二室开放模型,而静注8102(50mg/kg)其药-时曲线符合三室模型。肌注和静注8102其分上T1/2a分别为0.5和0.55h,消除相T1/2β分别为74.23和36.。31h。大鼠注8 相似文献
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采用生物鉴定法测定甲基黄酮醇胺(MFA)对PGI2和TXA2样物质生成的影响。以血小板聚集率%表示大鼠颈总动脉环PGI2活性,MFA(24.8μmol/kg,iv)组,阿斯匹林(ASA,0.83mmol/kg,iv)组和溶媒对照组分别为0.47±0.19、0.18±0.16、0.50±0.13/mg,结果提示:MFA不抑制TXA2的生成(P<0.05);ASA明显抑制PGI2的生成(P<0.01)。采用表面灌流法,AA为诱导剂,以兔主动脉条收缩强度(g)表示TXA2样物质的活性,MFA(10mmol/L)组,咪唑(1.67mmol/L)组和溶媒对照组分别为0.38±0.13、0.17±0.09和0.69±0.22g,结果提示MFA和咪唑抑制TXA2样物质的生成(P<0.01)。采用放射免疫法测定,MFA6.2μmol或12.4μmol/kg能明显抑制心肌梗塞家兔TXB2的升高(P<0.01),而对6-keto-PGF1α无明显影响 相似文献
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丹皮总甙和白芍总甙对自由基清除作用的影响 总被引:17,自引:0,他引:17
利用核黄素与光作用产生O2,Fenton反应产生OH·,以及H2O2引起的红细胞氧化溶血的方法,研究了丹皮总甙(TGM)和白芍总甙(TGP)对O2及OH·的清除作用和对溶血反应的影响。结果TGM和TGP对O2产生50%清除率时的浓度分别为2.31mg/L和2.64mg/L,对OH·产生50%清除率时的浓度分别为25.69mg/L和117.96mg/L,抑制H2O2引起溶血反应的IC50分别是0.94mg/L和1.41mg/L。结果表明TGM和TGP对自由基有清除作用,并能抑制H2O2引起的溶血反应,且TGM的作用强于TGP。 相似文献
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目的:探讨空腹TG正常的人在脂肪餐后的血脂水平的动态变化。方法:采用63.9g/m2体表面积的脂肪餐,对58名TG ≤1.70 mmol/L的人进行脂肪代谢的研究,根据年龄分为老年组、中年组和青年组,又依据餐后4h、8hTG浓度和TG-AUC分为餐后TG正常组和异常组。结果:脂肪餐后的TG水平明显较空腹增高(P <0.001),餐后各时间点之间的TG无显著性差异(P >0.05),平均达峰时间为3.98h,8h未能完全恢复至空腹水平。青、中、老年组达峰时间和TG-AUC依次递增,仅青年组和老年组达峰时间的差异有显著性意义(P <0.05)。餐后TG异常组各时间点的TG水平均明显高于正常组(P <0.001)。餐后TC、HDL-C和LDL-C在2 h时都有一个低谷期,8 h时基本恢复正常。结论:空腹TG正常者中有一些人,在高脂餐后可出现TG代谢异常;TG廓清速率随着年龄的增加而减慢。 相似文献
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内源性高甘油三酯血症(HTG)常伴血浆HDL-C含量下降及HDL组成异常。为了研究HTG患者血浆HDL亚类的改变,作者建立了密度梯度聚丙烯酰胺梯度凝胶电泳法(GGE),对25例HTG患者(血浆TG>2.26mmol/L,TC<6.72mmol/L)及25例年龄、性别匹配的血脂正常者(血浆TG<1.69mmol/L,TC<5.17mmol/L)的HDL亚类——HDL2a、HDL2b、HDL3a、HDL3b和HDL3c进行了分析,同时对血浆及HDL中脂质及载脂蛋白(apo)AⅠ、AⅡ、B100、CⅡ、CⅢ及E进行了测定。结果发现,HTG患者空腹血浆TG、apoB100、apoCⅡ、apcCⅢ及apoE显著增加(P<0.001),HDL-C及apoAⅠ显著降低(P<0.001及P<0.05);患者血浆HDL组成异常,HDL中TG含量显著增加(P<0.001),TC、apoAⅠ、apoCⅡ、apoCⅢ及apoE含量下降(P<0.01);患者血浆HDL亚类含量改变,HDL2b显著降低(P<0.02),HDL2a及HDL3a增加(P<0.05),且随血浆TG含量的升高而升高,HDL3b及HDL3c则未见改变 相似文献
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马钱子碱的药代动力学 总被引:7,自引:0,他引:7
用荧光分光光度法测定马钱子碱在小鼠体内的血药肖度及其药代动力学参数。结果表明:小鼠尾静脉注射3种剂量10,7.5和5mg.kg^-1的B后时量曲线均符合二室开放模型。其主要动力学参数:T^1/2a分别为0.17,0.22和0.1h;T1/2β分别为1.96,2.11和2.06h;Vc分别为7.23,7.59和56.65L.kg^-1AUC分别为2.48,1.96和1.45h.mg。;^-1;CLs 相似文献
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Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P < 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (>0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P <0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P < 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD. 相似文献
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Jiageng Zhu Jun Li Ruipeng Ji Jianghao Su Mingshun Shen Zhigang Cao 《南京医科大学学报(自然科学版)》2007,21(2):125-128
Objective: To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods: SD rats served as both donors and recipients. 4℃ sodium lactate Ringer's was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava (IVC) inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results: Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion: This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment. 相似文献
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Tao Ye * Zhi-quan Liu Jian-jun Mu Xi-han Fu Jun Yang Bao-lin Gao and Xiao-hong Zhang Department of Cardiology First Hospital of Xi'an Jiaotong University Xi'an Hanzhong Cardiovascular Institute Hanzhong 《中国医学科学杂志(英文版)》2004,19(4):248-251
Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P< 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P< 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure. 相似文献
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目的:评价使用安心颗粒对急诊经皮冠状动脉介入术(PPCI)术后生活质量的影响.方法:将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组(术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次)和对照组(仅接受基础药物治疗).所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表(MIDAS)、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果:入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异(P>0.05);出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低(P<0.05);组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低(P<0.05).两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血(P>0.05).两组发生轻度血小板减少症的患者数比较无差异(P>0.05).结论:PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险. 相似文献
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Jingming Zhu Ningning Hui Xiuhong Jiang Huaxing Zhang Chengzhang Fu 《南京医科大学学报(自然科学版)》2007,21(6):386-389
Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P<0.01);the MAP decreased(P<0.01)and the HR increased drastically(P<0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients. 相似文献
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Objective:To investigate the gene expression of osteoprotegerin(OPG) and osteoclast differentiation factor(ODF) in the bone tissue of patients with hip fracture due to osteoporosis. Methods:OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers(over 50 years old) with hip fracture(Observer Group) and 30 cases of hip facture sufferers with no osteoporosis(Control group) were analyzed with the Semi-Quantitative RT-PCR method. Results:The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion:Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption. 相似文献
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The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas: report of 9 cases 总被引:1,自引:0,他引:1
Shaoqin Chen Shengquan Zou Qibao Dai Peisheng Huang Weihua Cai Hong Li 《南京医科大学学报(自然科学版)》2007,21(6):398-401
Objective:To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas(SPTP). Methods:Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin(HE) staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results:Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin(9/9 cases), AAT(9/9 cases), NSE(9/9 cases), ACT(7/9 cases), CK20(2/9 cases), CgA(1/9 cases), S-100(3/gcases), PR(4/gcases), Syn(3/9 cases) and CD56(5/9cases), negative in CEA and ER. Conclusion:SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype. 相似文献
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Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine
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A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care(~'4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes. 相似文献
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Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P. 相似文献
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Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine. 相似文献