Expressions of miR-22 and miR-135a in acute pancreatitis

This study examined the expressions of miR-22 and miR-135a in rats with acute edematous pancreatitis （AEP） and their target genes in order to shed light on the involvement of miR-22 and miR-135a in the pathogenesis of acute pancreatitis （AP）. The in vivo model of AEP was established by introperitoneal injection of L-arginine （150 mg/kg） in rats. The miRNA microarray analysis was used to detect the differential expression of miRNAs in pancreatic tissue in AEP and normal rats. The in vitro AEP model was established by inducing the rat pancreatic acinar cell line （AR42J） with 50 ng/mL re- combinant rat TNF-ct. Real-time quantitative RT-PCR was employed to detect the expression of miR-22 and miR-135a in AR42J cells. Lentiviruses carrying the miRNA mimic and anti-miRNA oligonucleotide （AMO） of miR-22 and miR-135a were transfected into the AR42J cells. The AR42J cells transfected with vehicle served as control. Western blotting was used to measure the expression of activated cas- pase3 and flow cytometry analysis to detect the apoptosis of AR42J cells. Targets of miR-22 and miR-135a were predicted by using TargetScan, miRanda, and TarBase. Luciferase reporter assay and quantitative real-time RT-PCR were performed to confirm whether ErbB3 and Ptk2 were the target gene of miR-22 and miR-135a, respectively. The results showed that the expression levels of miR-22 and miR-135a were obviously increased in AEP group compared with the control group in in-vivo and in-vitro models. The expression levels of miR-22 and miR-135a were elevated conspicuously and the expression levels of their target genes were reduced significantly in AR42J cells transfected with Ienti- viruses carrying the miRNA mimic. The apoptosis rate was much higher in the TNF-ct-induced cells than in non-treated cells. The AR42J cells transfected with miRNA AMOs expressed lower level of miR-22 and miR-135a and had lower apoptosis rate, but the expression levels of ErbB3 and Ptk2 were increased obviously. It was concluded that the expression levels of miR-22 and miR-135a were elevated in AEP. Up-regulating the expression of miR-22 and miR-135a may promote the apoptosis of pancreatic acinar cells by repressing ErbB3 and Ptk2 expression in AEP.     

MicroRNA-34a inhibits human brain glioma cell growth by down-regulation of notch1

The effects of microRNA-34a (miR-34a)-regulated Notch1 gene on the proliferation and apoptosis of the human glioma cell line U87 were investigated in this study. The U87 cells were divided into miR-34a mimics, negative control, mock transfection and blank control groups in terms of different treatments. In miR-34a mimics group, human U87 glioma cells were transfected with miR-34a mimics by using lipofectamine 2000. The cells transfected with nonsense microRNA were set up as negative control group. Those treated with lipofectamine 2000 only were designated to the mock tranfection group. In the blank control group, the cells were cultured routinely and no treatment was given. The expression of miR-34a and Notch1 was detected by using real-time RT-PCR. Western blotting was employed to monitor the change in Notch1 protein. Cell proliferation and apoptosis were measured by CCK-8 and flow cytometry. The results showed that the proliferative ability of U87 cells was significantly reduced and the apoptotic cells increased in miR-34a mimics group relative to control groups. The expression of miR-34a was significantly up-regulated in mimics group as compared with control groups (P<0.05). Furthermore, Notch1 protein levels were significantly decreased in miR-34a mimics group when compared with control groups (P<0.05), but the mRNA expression of Notch1 showed no significant difference among these groups. It was concluded that miR-34a may suppress the proliferation and induce apoptosis of U87 cells by decreasing the expression of target gene Notch1, suggesting that miR-34a may become a promising gene therapeutic target for brain glioma.     

Mitofusin2 decreases intracellular cholesterol of oxidized LDL-induced foam cells from rat vascular smooth muscle cells

Mitofusin2 (Mfn2) plays a pivotal role in the proliferation and apoptosis of vascular smooth muscle cells (VSMCs). The purpose of this study was to investigate the effects of Mfn2 on the trafficking of intracellular cholesterol in the foam cells derived from rat VSMCs (rVSMCs) and also to investigate the effects of Mfn2 on the expression of adenosine triphosphate-binding cassette subfamily A member 1 (ABCA1), adenosine triphosphate-binding cassette subfamily G member 1 (ABCG1) and peroxisome proliferator-activated receptor gamma (PPARγ). The rVSMCs were co-cultured with oxidized low density lipoprotein (LDL, 80 μg/mL) to produce foam cells and cholesterol accumulation in cells. Before oxidized LDL treatment, different titers (20, 40 and 60 pfu/cell) of recombinant adenovirus containing Mfn2 gene (Adv-Mfn2) were added into the culture medium for 24 h to transfect the Mfn2 gene into the rVSMCs. Then the cells were harvested for analyses. The protein expression of Mfn2 was significantly higher in Adv-Mfn2-transfected group than in untransfected group (P<0.05), and the expression levels significantly increased when the titer of Adv-Mfn2 increased (P<0.05). At 24 or 48 h after oxidized LDL treatment, rVSMCs became irregular and their nuclei became larger, and their plasma abounded with red lipid droplets. However, the number of red lipid droplets was significantly decreased in Adv-Mfn2-transfected group as compared with untransfected group. At 48 h after oxidized LDL treatment, the intracellular cholesterol in rVSMCs was significantly increased (P<0.05), but it was significantly decreased in Adv-Mfn2-transfected group as compared with untransfected group (P<0.05), and it also significantly decreased when the titer of Adv-Mfn2 increased (P<0.05). The mRNA and protein expression levels of ABCA1 and ABCG1 were significantly increased in Adv-Mfn2-transfected group as compared with untransfected group (P<0.05). Though the mRNA and protein expression levels of PPARγ was not significantly increased (P>0.05), the phosporylation levels of PPARγ were significantly decreased in Adv-Mfn2-transfected group as compared with untransfected group (P<0.05). These results suggest that the transfection of Adv-Mfn2 can significantly reduce intracellular cholesterol in oxidized LDL-induced rVSMCs possibly by decreasing PPARγ phosporylation and then increasing protein expression levels of ABCA1 and ABCG1, which may be helpful to suppress the formation of foam cells.     

Effect of progesterone combined with chemotherapy on epithelial ovarian cancer

Objective To identify an effective auxiliary therapy for epithelial ovarian cancer. Methods Progesterone acetate given at 250 mg intramuscularly twice a week for 1 month followed by increased administration to 500 mg intramuscularly every two weeks for 3 years was used in combination with platinum based chemotherapy to treat patients with epithelial ovarian cancer as a first-line therapy. Prognoses of the patients receiving progesterone combined with chemotherapy (progesterone group) and those receiving chemotherapy only (control group) were compared. Results Three-year recurrence and survival conditions of the progesterone and control groups were as follows. Stage Ⅰa:no patient relapsed or died in either group. Stage Ⅰb-Ⅰc:three-year recurrence rates were 14.2% and 37.5%, respectively ( P=0.2845); three-year survival rates were 92.3% and 87.5% (P=0.7221). Stage Ⅱ: 1 patient relapsed and died among the 3 patients in the progesterone group; among the 4 patients in the control group, 1 patient relapsed, none died. Stage Ⅲ: three-year recurrence rates were 30.8% and 64.3%, respectively (P=0.1170); three-year survival rates were 85.7% and 42.9%, respectively (P=0.005). Stage Ⅳ: 4 patients relapsed and 1 patient died among the 7 patients in the progesterone group; both the patients in the control group relapsed and died. Conclusions The results indicated that progesterone combined with platinum based chemotherapy as a first-line therapy may improve the prognosis of advanced epithelial ovarian cancer, but would not change the prognosis of early stage epithelial ovarian cancer.     

Effects of Yixin Jiangya Capsules on Insulin Resistance and Tumor Necrosis Factor-a in Cases of Primary Hypertension with Left Ventricular Hypertrophy

To observe the effects of Ftxin Jiangya Capsules （益心降压胶囊capsules for nourishing the heart and lowering blood pressure） on insulin resistance （IR） and tumor necrosis factor-a （TNF-a） in patients with primary hypertension with left ventricular hypertrophy （LVH）. Methods： Totally 93 cases were randomly divided into a control group of 31 cases taking Enalapril and a treatment group of 62 cases taking Enalapril and Irtxin Jiangya Capsules. Results： Fasting serum insulin （FSI） and TNF-a obviously increased and insulin sensitive index （ISI） significantly decreased in both groups before treatment as compared to those of a healthy group. After treatment, FSI, TNF-a and fasting blood glucose （FBG） obviously decreased and ISI remarkably increased in the treatment group, while ISI significantly increased and TNF-a obviously decreased in the control group. The curative effect in the treatment group was remarkably superior to that in the control group. FSI was positively related to TNF-a before treatment in both groups. Conclusion： FSI and TNF-a obviously increase and ISI significantly decreases in patients with primary hypertension with LVH. FSI and TNF-a influencing each other are involved in the generation and development of hypertension. Irtxin Jiangya Capsules can improve IR and decrease TNF-a.     

Increased procoagulant activity of red blood cells in the presence of cisplatin

Background Cisplatin based chemotherapy is a well recognized risk factor for coagulation disorders and thrombosis. The pathophysiological mechanisms by which cisplatin promote thrombosis are not well understood. Methods Red blood cells （RBCs） were separated from peripheral blood of patients with breast cancer （n=10） and healthy adults （n=6） and treated with cisplatin. Coagulation time of RBCs was assessed by one step recalcification time and the productions of thrombin, intrinsic and extrinsic factor Xa were measured in the presence or absence of various concentrations of lactadherin. Exposed phosphatidylserine was stained with lactadherin and observed by confocal microscopy and flow cytometry. Results Neither fresh RBCs nor RBCs treated without cisplatin had potent procoagulant activity. Cisplatin treatment increased procoagulant activity of RBCs in a cell number- and concentration-dependent manner. Exposed phosphatidylserine was stained with lactadherin and after cisplatin treatment, strong fluorescence was revealed by confocal microscopy. Lactadherin bound RBCs from patients with breast cancer increased from （1.9＋0.5）% on control RBCs to （68.0±3.5）% on RBCs treated with 10umol/L cisplatin for 24 hours. Conclusions Cisplatin treatment increases procoagulant activity of RBCs, which have a strong association with exposure of phosphatidylserine. The increased procoagulant activity may contribute to the pathogenesis of thrombophilia during cisplatin based chemotherapy in breast cancer patients.     

Genomewide DNA Methylation Responses in Patients with β-Thalassemia Treated with Yisui Shengxue Granules (益髓生血颗粒)

Objective: To examine the clinical effects of Yisui Shengxue Granules(益髓生血颗粒) in the treatment of β-thalassemia and explore its mechanism on DNA methylation levels. Methods: A randomized placebo-controlled double-blinded trial was conducted. Forty patients with β-thalassemia were recruited and distributed randomly by envelope method into an experimental group and a control group, 20 patients in each group. The patients were given Yisui Shengxue Granules in the experimental group and placebo in the control group(12 g/bag, 3 times a day) during a 3-month intervention. Before and after 1, 2, and 3 months of treatment, peripheral intravenous blood was sampled, and blood parameters such as hemoglobin(Hb), red blood cells(RBCs), reticulocytes(Ret), and fetal hemoglobin(HbF) were analyzed. Mononuclear cells from 5 patients, who showed an obvious treatment effect, were isolated by density gradient centrifugation. DNA methylation was analyzed using an Affymetrix USA GeneChip Human Promoter 1.0 Array and Input-promoter 1.0. Results: Compared with pre-treatment, there was an obvious increase in Hb and RBCs counts after 1, 2, and 3 months in the experiment group(P0.01 or P0.05). Meanwhile, HbF increased from the 2 nd to the 3 rd month(P0.05). In the control group, Hb and RBCs showed no obvioas change. After 3-month treatment, DNA methylation results from 5 patients revealed that there were 24 hypomethylated genes and 3,685 hypermethylated genes compared with pre-treatment. Genes of insulin-like growth factor 1 receptor(IGF1 R) and Janus kinase 3(JAK3) revealed the most relations with other genes(degree: 21) and genes of 1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase gamma 2(PLCG2) and mitogen-activated protein kinase 10(MAPK10) showed a stronger intermediary role(betweenness centrality=0.04). Conclusions: JAK3 and MAPK10 are two key genes in bone marrow and the lymphatic system, and JAK3 is likely to be related to hematopoietic cytokines in the process of early hematopoiesis.(Registration No. NCT01549080)     

Tiaobu Xinshen Recipe (调补心肾方) Improved Mild Cognitive Impairment of Alzheimer's Disease Patients with Xin (Heart) and Shen (Kidney) Deficiency

Objective: To observe the intervention effects of Tiaobu Xinshen Recipe(调补心肾方, TXR) on patients with mild cognitive impairment caused by Alzheimer's disease(MCI-AD). Methods: Totally 88 MCI-AD patients with syndrome of Xin(Heart) and Shen(Kidney) deficiency were assigned to the experimental group(47 cases, treated with TXR) and the control group(41 cases, treated with donepezil hydrochloride) using a random number table. Final recruited qualified patients were 44 cases in the experimental group and 39 cases in the control group. The therapeutic course was 12 weeks. Neuropsychological scales [mini mental state examination(MMSE) and Montreal cognitive assessment(MoCA)], and Chinese medicine(CM) dementia syndromes scales were performed in all patients, and results were compared between groups or intra-group before and after treatment. Results: MMSE and Mo CA scores of the two groups were increased after treatment compared with those before treatment(P0.05). But there was no statistical difference in MMSE or MOCA scores after treatment between the two groups(P0.05). CM dementia syndrome score was significantly decreased after treatment in the experimental group compared with the control group(P0.01). Visual spatial and executive function scores and delayed recall scores of the two groups were increased compared with those before treatment(P0.01). Conclusion: TXR could effectively improve cognitive impairment of MCI-AD patients with syndrome of Xin and Shen deficiency.     

Evaluation of oxidative stress in colorectal cancer patients

Objective To evaluate the oxidative stress in patients with colorectal cancer and to investigate the relationship between oxidative stress and colorectal cancer. Methods Seventy-six subjects were divided into two groups （36 colorectal cancer patients as the study group and 40 normal healthy individuals as the control group）. Their protein oxidation, DNA damage, lipid peroxidation and antioxidants, vitamin C, vitamin E, glutathione （GSH）, and antioxidative enzymes in serum were detected. Results The levels of protein carbonyl and advanced oxidation protein products （AOPP） were significantly higher in the study group than in the control group （P〈0.01）. Serum 8-OHdG was significantly increased in the study group compared to the control group （P〈0.01）. However, the mean serum level of MDA and conjugated diene was lower in the study group than in the control group （P〈0.01）. The activity of antioxidative enzymes was significantly decreased in the study group compared to the control group （P〈0.01）. Serum vitamins C and E concentrations were significantly reduced in the study group compared to the control group （P〈0.01）. Conclusion Colorectal cancer is associated with oxidative stress, and assessment of oxidative stress and given antioxidants is important for the treatment and prevention of colorectal cancer.     

特应性皮炎患者血浆外泌体miR-21和lncRNA MALAT1的检测水平及意义

  目的  分析血浆外泌体微小RNA-21(miR-21)、长链非编码RNA(lncRNA)肺癌转移相关转录本1(MALAT1)在特应性皮炎(AD)患者中的表达水平,探讨二者的临床意义。  方法  选择2018年1月—2020年5月宁波市医疗中心李惠利医院皮肤科收治的AD患者118例,依据特应性皮炎积分指数(SCORAD)将患者分为轻度41例、中度38例、重度39例,另选取同期健康体检者120例为对照组。提取并鉴定血浆外泌体;采用实时荧光定量PCR法(qRT-PCR)测定血浆外泌体中miR-21、lncRNA MALAT1水平;Pearson法分析血浆外泌体miR-21、lncRNA MALAT1水平与SCORAD评分的相关性;多元线性逐步回归模型分析AD患者SCORAD评分增加的危险因素。  结果  所提取样品具备外泌体特征;AD组轻、中、重度患者血浆外泌体中miR-21、lncRNA MALAT1水平、SCORAD评分显著高于对照组,且随病情严重程度增加而升高(F=91.160、206.502、246.615,均P＜0.001)。血浆外泌体miR-21、lncRNA MALAT1水平与SCORAD评分均呈正相关关系(r=0.631、0.528,均P＜0.05)。血浆外泌体miR-21、lncRNA MALAT1水平高均为AD患者SCORAD评分增加的危险因素(均P＜0.05)。  结论  血浆外泌体miR-21、lncRNA MALAT1异常高表达可能与AD发生发展有关,且与病情严重程度关系密切,监测二者水平变化可能对进一步靶向防治AD发生有一定的意义。      

紫杉醇耐药乳腺癌细胞外泌体来源的miR-5585-5p诱导乳腺癌细胞产生耐药表型研究

目的研究紫杉醇耐药乳腺癌细胞外泌体来源的miR-5585-5p对乳腺癌细胞迁移、凋亡和耐药性的影响。方法超速离心法获取外泌体,使用qRT-PCR方法分别检测乳腺癌细胞（SKBR-3）和紫杉醇耐药乳腺癌细胞（SKBR-3/PR）细胞株和外泌体中miR-5585-5p的表达量;通过外泌体共培养技术以及在乳腺癌耐药细胞中转染miR-5585-5p的抑制剂,用Transwell检测其迁移能力,流式细胞术检测其凋亡情况;qRT-PCR和Western blotting检测其耐药指标。结果与SKBR-3相比,miR-5585-5p在耐药细胞SKBR-3/PR细胞株和外泌体中表达均上调（P < 0.01和P < 0.05）;在耐药细胞株中转染miR-5585-5p的抑制剂,Transwell、流式分析等结果显示,与对照相比,抑制剂组的耐药性下降,生物学活性降低（P < 0.01）;耐药细胞源性的外泌体与亲本细胞共培养可使SKBR-3细胞产生耐药表型,增强其迁移能力,抑制其凋亡率（P < 0.01）,而转染抑制剂的耐药细胞分泌的外泌体可减弱这一作用。结论紫杉醇耐药乳腺癌细胞通过外泌体递送高表达的miR-5585-5p至亲本细胞并诱导亲本细胞产生耐药表型,外泌体miR-5585-5p可为乳腺癌耐药治疗提供新的分子靶点,为乳腺癌预后评估提供新的生物标志物。     

阿尔茨海默病与轻度认知功能障碍患者精神行为症状比较分析

  目的  通过分析阿尔茨海默病（Alzheimer’s disease, AD）与轻度认知功能障碍（mild cognitive impairment,MCI）患者精神行为症状的异同,为阿尔茨海默病与轻度认知功能障碍的精神行为症状（behavioral and psychological symptoms of dementia,BPSD）诊疗提供指导。  方法  采用神经精神问卷（neuropsychiatric inventory,NPI）评估来自云南省的71例AD患者、31例MCI患者精神行为症状的发生率、严重程度,并与70例正常对照者比较。  结果  抑郁、焦虑、易激惹的发生率及严重程度,AD与MCI组比较差异无统计学意义（P > 0.05）,而MCI组高于正常对照组（P < 0.05）。淡漠发生率AD组与MCI组比较差异无统计学意义（P > 0.05）,淡漠严重程度AD组高于MCI组（P < 0.05）;而MCI组淡漠发生率及严重程度高于正常对照组（P < 0.05）。NPI总分、其余症状发生率或严重程度AD组高于MCI组（P < 0.05）,而MCI组发生率或严重程度与正常对照组比较差异无统计学意义（P > 0.05）。  结论  AD患者总体BPSD较MCI患者严重,而抑郁、焦虑、易激惹症状在AD与MCI患者之间无差异,MCI处于正常衰老与AD之间的认知变化过渡阶段,伴有这些症状的MCI患者需要重视,及早干预。     

TWEAK诱导巨噬细胞源性外泌体miRNA抑制膀胱癌转移的研究

目的:研究肿瘤坏死因子样弱凋亡诱导因子(tumor necrosis factor-like weak inducer of apoptosis,TWEAK)诱导的肿瘤相关巨噬细胞(TMs)源性的外泌体对膀胱癌细胞的转移影响.方法:使用TWEAK刺激THP-1诱导分化的TMs,检测其外泌体的特征.并使用miRNAs芯片...     

骨桥蛋白及炎性因子在轻度认知障碍进展的预测价值研究

目的　评估骨桥蛋白（OPN）以及炎性因子在轻度认知障碍（MCI）患者进展为阿尔茨海默病（AD）中的预测价值。方法　前瞻性分析2015年6月—2017年6月新疆维吾尔自治区中医医院脑病一科收治的MCI患者以及年龄、性别相匹配的非炎症性神经病变（OND）患者,各60例。收集患者临床资料并对其进行病情评估,采用酶联免疫吸附试验（ELISA）检测患者脑脊液和血清OPN水平、血清白介素（IL）-1β、IL-6、IL-17A、转化生长因子（TGF）-β1、肿瘤坏死因子（TNF）-α和IL-4水平。对患者通过门诊和住院随访1年,根据患者在随访结束时的诊断分为AD组、未进展组和OND组。再次检测随访结束时AD组患者血清OPN以及炎性因子水平。结果　随访结束时AD组23例,未进展组32例,OND组51例。入院时3组脑脊液OPN水平比较,差异有统计学意义（P<0.05）;其中AD组脑脊液OPN水平高于未进展组和OND组（P<0.05）。随访1年时,3组IL-1β、IL-6、IL-17A、TGF-β1、IL-4水平比较,差异均无统计学意义（P>0.05）;随访1年时3组血清OPN、TNF-α水平比较,差异均有统计学意义（P<0.05）;其中AD组血清OPN、TNF-α水平均高于未进展组和OND组（P<0.05）。相关性分析结果显示,所有患者入院时脑脊液OPN、血清OPN水平与MMSE评分呈正相关（r=0.348,P<0.001;r=0.631,P<0.001）,血清TNF-α水平与MMSE评分呈负相关（r=-0.435,P<0.001）。AD组患者入院时、诊断AD时、随访1年时MMSE评分、脑脊液OPN、血清OPN水平比较,差异均有统计学意义（P<0.001）;其中诊断AD时、随访1年时MMSE评分低于入院时,脑脊液OPN、血清OPN水平高于入院时（P<0.05）。结论　脑脊液OPN水平是早期识别进展为AD的MCI患者的潜在生物标志物,并且血清及脑脊液OPN水平以及TNF-α与AD的痴呆严重程度具有相关性。     

vMIP-ⅡN端肽对趋化因子受体CXCR4表达调控的机制研究

目的:探讨病毒巨噬细胞炎性蛋白Ⅱ（vMIP-Ⅱ）对乳腺癌细胞CXCR4表达的影响及分子机制。方法:设计并合成扩增CXCR4核心启动子基因序列,构建荧光素酶报告载体,酶切鉴定;通过检测报告基因荧光素酶活性反映病毒巨噬细胞炎性蛋白N端21肽（NT21MP）对CXCR4启动子的作用;通过荧光定量PCR技术和Western blot检测CXCR4干扰或过表达、HER-2干扰,以及miRNAs抑制剂和类似物转染后相关基因及miRNAs表达;采用磺酰罗丹明B（SRB）染色法检测细胞增殖活性,PI染色法检测细胞周期,Annexin V/PI染色法检测细胞凋亡。结果:NT21MP下调CXCR4的表达,并上调miR-125b、miR-135a和miR-146a的表达（P < 0.05~P < 0.01）;NT21MP降低CXCR4核心启动子的活性;SP1为CXCR4核心启动子高频结合转录因子,miR-135a可下调SP1的表达（P < 0.01）;过表达CXCR4上调HER-2表达（P < 0.01）,而干扰CXCR4表达则下调HER-2表达（P < 0.01）;miR-125b可下调HER-2的表达（P < 0.01）;HER-2表达下调诱导miR-146a的表达;miR-146a抑制CXCR4表达（P < 0.01）;相对于空白对照组,miR-146a和miR-135a均可抑制细胞增殖,阻断细胞周期,并诱导凋亡（P < 0.01）。结论:NT21MP通过诱导miR-125b、miR-135a和miR-146a负调控CXCR4的表达,从而抑制乳腺癌细胞的增殖能力,为NT21MP应用于乳腺癌患者的治疗提供了理论基础。     

轻度认知障碍和阿尔茨海默病患者血白细胞介素-6水平变化

目的观察轻度认知障碍（MCI）和阿尔茨海默病（AD）患者血清白细胞介素-6（IL-6）水平变化。方法采用双抗体夹心酶联免疫吸附试验（ELISA）法检测AD 14例、MCI 14例和对照组11例的血清IL-6水平。结果 3组血清IL-6水平比较差异有统计学意义（P〈0.05）,MCI组和AD组血清IL-6水平均高于对照组（P〈0.05）,MCI组与AD组相比差异无统计学意义（P〉0.05）。结论 MCI及AD均有炎症改变,提示炎症反应可能是AD发病机制中的一个重要环节,在AD进一步病理变化过程中可能起重要作用。     

阿尔茨海默病、轻度认知障碍、健康老人脑电图及神经心理学测试比较研 究简

目的 比较阿尔茨海默病（AD）、轻度认知障碍（MCI）、健康老人脑电图（EEG）及神经心理学测试（MMSE）特点.方法 选择2012年1月～2013年1月海军总医院海空勤体检中心进行检查的老年MCI患者115例为MCI组,选择同期AD患者共105例为AD组,健康体检老年人80名为对照组.三组均进行EEG检查和MMSE测试并比较检查结果.结果 ①MCI组EEG总异常61例（53.0％）,AD组EEG总异常98例（93.3％）,对照组EEG总异常24例（30.0％）,三组EEG总异常发生率比较,差异有高度统计学意义（x2-87.87,P＜0.01）.②三组MMSE评分中,时间、地点定向,即刻记忆,计算、回忆、命名、复述、阅读、结构、书写能力,三步命令,总分等项目差异均有统计学意义（P＜ 0.05或P＜0.01）.两两比较中,MCI组地点定向,计算、回忆、结构、书写能力评分与对照组比较,差异均有统计学意义（P＜ 0.05或P＜0.01）;而AD组时间、地点定向,即刻记忆,计算、回忆、命名、复述、阅读、结构、书写能力,三步命令等各项评分与对照组比较差异均有统计学意义（P＜ 0.05或P＜0.01）,而与MCI组比较差异无统计学意义（P＞0.05）.MCI组和AD组MMSE总分与对照组比较,差异均有统计学意义（P＜ 0.05或P＜ 0.01）.结论 EEG检查并结合MMSE量表评价可对MCI患者做出较为准确的评价,是能够早期对MCI做出诊断的有效方法.     

阿尔茨海默病和轻度认知障碍血CD4~＋T细胞计数变化

目的观察阿尔茨海默病（AD）和轻度认知障碍（MCI）患者外周血CD4＋T细胞相对计数变化。方法采用流式细胞仪测定10例AD患者及14例MCI患者的CD4＋T细胞相对数,并与对照组11例比较。结果 AD组外周血CD4＋T细胞计数高于对照组（P〈0.01）和MCI组（P〈0.05）,MCI组与对照组相比差异无统计学意义（P〉0.05）。结论 CD4＋T细胞在AD炎症反应机制中起重要作用,但其对AD的影响作用还有待于进一步探讨。