HLC-723GX全自动糖化血红蛋白分析仪检测糖化血红蛋白在糖尿病诊断中的价值分析

目的:研究探讨HLC-723GX全自动糖化血红蛋白分析仪检测糖化血红蛋白在糖尿病诊断中的价值,总结临床治疗经验。方法:选自本院2019年6月~12月收治的30例糖尿病患者作为研究组,选择本院门诊同期老年健康体检者30例作为对照组。分析HLC-723GX全自动糖化血红蛋白分析仪HbAlc的精密度、准确度,检测两组的糖化血红蛋白(Glycated hemoglobin,HbAlc)的结果,比较其诊断价值。结果:待评检测系统HbA1c批内、批间CV均符合要求,精密度达标;待评检测系统与目标检测系统检测结果偏倚<1/2TEa,准确度达标;对照组患者HbAlc的阳性率为0,研究组患者HbAlc的阳性率为96.67%,其中1例患者HbAlc值无明显异常。结论:HLC-723GX全自动糖化血红蛋白分析仪检测糖化血红蛋白不但准确度很高,同时具有较高的精密度,能够根据最终的糖化血红蛋白值进行糖尿病的诊断。     

DS5糖化血红蛋白分析仪的性能评价

目的:对DS5糖化血红蛋白分析仪进行性能评价。方法:系统研究DS5糖化血红蛋白分析仪测定糖化血红蛋白的精密度灵敏度、准确性并分析其与手工微柱法结果的相关性。结果:DS5糖化血红蛋白分析仪检测HbAlc具有较高的精密度,批内CV为1.94%,批间CV为3.05%,平均回收率为98.2%,与手工微柱法比较,r=0.935(P<0.005)。两法具有高度的相关性。正常人群的HbAlc范围为4.5±2.2%。糖尿病病人的HbAlc测定值为8.3%～23.0%。结论:DS5糖化血红蛋白分析仪具有灵敏、准确、快速等特点。     

妊娠中、晚期糖化血红蛋白及糖化血清蛋白水平与妊娠糖尿病的关系

目的:比较正常妊娠组与妊娠糖尿病组(GDM)的糖化血红蛋白水平,探讨糖化血红蛋白与妊娠糖尿病之间的关系。方法:选择2010年2月～2011年11月257例妊娠期糖尿病及妊娠合并糖尿病患者,同时选取257例正常妊娠产妇作为对照组,应用免疫比浊法对两组受试者糖化血红蛋白含量进行测定,所用仪器是日立7170A生化仪。结果:妊娠糖尿病(GDM)患者的糖化血红蛋白(HbA1c)明显高于正常妊娠组(P<0.05)。GDM组HbA1c出现异常有105例,异常率为40.85%;正常妊娠组HbA1c出现异常28例,异常率为10.89%。GDM组HbA1c异常率明显高于正常妊娠对照组(P<0.05)。结论:糖化血红蛋白的测定可以作为妊娠糖尿病的辅助诊断手段,其操作简单、结果可靠,具有很好的临床应用价值。     

HA-8160全自动糖化血红蛋白分析仪检测系统的性能验证

目的:对日本ARKRAY公司HA-8160型全自动糖化血红蛋白分析仪检测系统的主要分析性能进行验证.方法:对该仪器检测项目的精密度、携带污染率及线性范围进行评价.结果:检测项目各精密度在可接受范围内；携带污染率在可接受范围内;线性验证标本按一定比例稀释后所得理论值与实测值的回归方程y=bx+a中,b值介于0.97～1.03范围内,a在可接受范围内.结论:HA-8160检测系统的精密度、携带污染率及线性范围基本符合实验诊断学实验室的要求.     

免疫比浊法测定糖化血红蛋白的方法评价

目的:对采用免疫比浊法对糖化血红蛋白进行测定的方法进行评价,为临床提供可靠地参考依据。方法:随机抽取40例患有糖尿病的患者和40例健康体检人群,对他们的糖化血红蛋白进行测定,并对检测结果进行比较分析,对采用免疫比浊法对糖化血红蛋白进行测定的方法进行评价。结果:正常组与糖尿病组的糖化血红蛋白结果分别为(5.17±0.38)%、(8.03±1.58)%,且具有明显的统计学差异(P<0.05)。对于糖尿病组控制好的约占33%左右,控制较好的约占17%左右,控制不足的占50%左右。结论:采用免疫比浊法对糖化血红蛋白进行测定结果符合在临床上对糖尿病患者的治疗检测条件,而且还为对治疗措施以及用药剂量进行及时的调整给予可靠地参考依据。     

三种非线性校准模式在糖化血红蛋白测定中的应用

目的:探讨免疫法糖化血红蛋白合适的校准方式。方法:分别用全自动生化分析仪中常用的三种非线性校准模式对糖化血红蛋白试剂进行校准,比较不同校准模式下测定结果的差异。结果:50份样本在Logit-log4P,spline和line graph三种校准模式下测定均值分别为8.17,8.15和8.27(P=0.417);HbA1c浓度梯度测定中Logit-log4P和spline两种校准模式检测结果极其符合,比对曲线几乎重合,而line graph校准方式由于标准曲线未充分拟合在高值区和高低值区与前二者相比存在较大偏差,高值偏高,低值偏低。结论:免疫法糖化血红蛋白校准方式应选择Logit-log4P和spline为宜。     

糖化血红蛋白即时检验在临床中的应用

目的:探讨糖化血红蛋白(HbA1C)即时检验(POCT)在临床中的应用。方法:糖化血红蛋白(HbAIC)即时检验(POCT)分析仪NycoCardReaderⅡ与测定HbA1C的参比方法高效液相离子层析法(HPLC)进行比对,结果进行相关分析,并判断偏倚是否可以接受。结果:结果预期偏倚均在要求范围内。结论:糖化血红蛋白(HbAIC)即时检验(POCT)与HPLC法相比呈良好的相关性,可以作为HPLC的替代方法用于糖化血红蛋白的检测,适合在临床中应用。     

酶法检测糖化血红蛋白在妊娠期糖尿病中的应用评价

目的:对酶法测定糖化血红蛋白(HbAIc)进行方法学评价,探讨其在妊娠期糖尿病(GDM)诊断中的临床意义.方法:系统研究酶法测定HbAIc的灵敏度、精密度及干扰因素.并将正常妊娠组和GDM组进行比较.结果:酶法测定HbAIc批内平均变异系数(CV)为3.36％,批间平均CV为3.51％,平均回收率99.40％.抗坏血酸低于4 mg/dl时对结果无干扰,血红蛋白(Hb)在2.0 g/L以下对测定结果无干扰,三酰甘油4.5 mmol/L以下对测定结果无干扰,与DS5糖化血红蛋白仪比较有良好的相关性,Y=0.895X+0.29,r=0.924 (P ＜0.01),GDM组结果高于正常妊娠组,差异具有统计学意义(P＜0.0l).结论:酶法测定HbAIc具有较高的灵敏度和精密度,适于大批量及临床常规使用.     

探讨两种不同糖化血红蛋白检测方法的相关性

目的验证日本第一化学药品株式会社生产的酶法糖化血红蛋白检测试剂(Norudia HbAlc)与采用美国糖化血红蛋白标准化计划(NGSP)标准的HPLC法之间的相关性。方法糖化血红蛋白检测试剂(NorudiaHbAlc)和BioRad VarianⅡ同时检测正常人(25例)、糖尿病患者(26例)以及贫血患者(9例)的糖化血红蛋白,并进行比较。结果使用JCCLS标准的Norudia HbAlc试剂在日立7080仪器测定的糖化血红蛋白,结果经公式NGSP(()=0.96*JCCLS(%)+0.62转换后和BioRad VarianⅡHPLC方法比对实验得出相关系数为0.99,验证了JCCLS和NGSP标准测定糖化血红蛋白可以使用公式相互转换。结论Norudia HbA1c试剂采用日本JCCLS标准,可以通过公式转换,将结果统一至NGSP方法。     

免疫凝集法测定糖化血红蛋白

目的：对免疫凝集法测定糖化血红蛋白（HbA1c）进行临床应用评价。方法：采用免疫凝集法测定糖化血红蛋白,同时用高铁血红蛋白法测定总血红蛋白,然后计算出糖化血红蛋白的百分比。结果：该方法批内、批间精密度在5%以内;同时用微柱层析法测定40份标本,两者有正相关性,回归方程是Y=1.032X-0.436,r=0.954,P〈0.01;两份混合标本回收率为98.7%和100.9%;甘油三脂、总胆红素、血红蛋白分别为8mmol/L、200μmol/L、60g/L时对本法无明显干扰。结论：该方法可用末梢血测定且不受饮食的影响,最大的优点是能进行自动化分析,因此具有快速、准确、重复性好、易于推广等特点。     

两种高密度脂蛋白胆固醇测定试剂盒的性能评价

目的比较免疫抑制直接法(AB法)与选择性抑制法(PPD法)两种试剂盒,评价其在测定高密度脂蛋白(HDL-C)上的性能。方法在全自动生化分析仪上,分别检测两种试剂的精密度、测定范围、抗干扰能力,以及临床测定标本结果的相关性。结果 AB法试剂和PPD法试剂的批内精密度均1%,批间精密度均2%,均有较好的精密度。AB法试剂测定范围为0.04~4.0mmol/L,PPD法试剂为0.06~2.60mmol/L。AB法试剂和PPD法试剂在干扰物质Hb5.0g/L时,对结果没有明显影响,在干扰物质TG13.0mmol/L时,用AB法试剂测定HDL-C没有明显影响,PPD法试剂在干扰物质TG≥13.0mmol/L时,检测结果偏差15%。两种试剂测量临床样本具有良好的相关性,回归方程为Y=0.867 X+0.164(r=0.982,P0.05)。结论 AB法试剂抗干扰能力优于PPD法试剂;两种试剂测定方法具有良好的相关性,符合临床检测要求。     

参加美国病理家学会抗磷脂抗体能力验证活动效果分析

目的汇总分析北京协和医院风湿免疫科实验室和全世界参加美国病理家学会(College of American Pathologists,CAP)抗磷脂抗体(antiphospholipid antibodies, aPLs)项目的能力验证(proficiency testing,PT)活动8年的效果,以不断提高监控实验室检测aPLs项目的能力和质量。 方法采用回顾性分析研究。北京协和医院风湿免疫科实验室自2009年1月至2016年12月,每年以邮件方式接收2批,每批3份检测样本,在10 d内采用酶联免疫吸附试验(enzyme-linked immununosorbent assay, ELISA)完成抗心磷脂抗体(anticardiolipin,aCL)、抗β2糖蛋白1抗体(anti-β2- glycoprotein 1,aβ2GP1)检测,并将结果回报CAP。 结果本实验室自2009年1月起至2016年12月止,参加CAP的aPLs项目PT活动16次,共96个检测结果,所有结果均为可接受,总PT成绩为100%。8年期间,全世界参加CAP的aPLs项目PT活动的实验室数量逐年增加,2009年第1次PT与2016年第2次PT相比,参加aCL-IgG PT活动的实验室数量增长了24.55%,参加aCL-IgM PT活动的实验室数量增长了24.53%,参加aCL-IgA PT活动的实验室数量增长了20.13%,参加aβ2GP1-IgG PT活动的实验室数量增长了179.73%,参加aβ2GP1-IgM PT活动的实验室数量增长了197.01%,参加aβ2GP1-IgA PT活动的实验室数量增长了151.16%。检测方法逐渐由单一的ELISA变为多种方法学,包括ELISA法、化学发光法、荧光酶免疫分析法和液态芯片技术。全世界的回报结果显示,高滴度阳性和阴性样本检测结果的一致性较好,均高于90%,而低、中等滴度阳性样本检测结果的一致性较差。 结论本实验室的aPLs项目检测结果达到了CAP的能力验证质量要求。全世界越来越多的实验室重视参与aPLs项目的室间质量评价活动,但弱阳性、中等强度阳性的aPLs样本的检测质量仍有待提高。     

结核分枝杆菌菌悬液光密度值与菌落计数的关联性

目的建立一种基于光密度值计算结核分枝杆菌菌落数的可靠方法。方法利用低频超声和玻璃珠研磨两种方法制备H37Ra菌悬液,菌悬液2倍梯度稀释后,分别测定各个稀释度菌悬液在600 nm处的光密度值（OD600值）,并分析OD600值与稀释倍数曲线,确定最佳的菌悬液制备方法,OD600线性范围,以及OD600值与CFU关联曲线。结果OD600值为0.1～0.6,线性范围内OD600值与稀释倍数线性回归分析结果显示,玻璃珠研磨法和低频超声法相关系数（R2）分别为0.98、1.00,均呈良好的相关性,且低频超声法比玻璃珠研磨法的相关性好,其菌液分散更均匀。OD600值与CFU值线性回归分析结果显示,玻璃珠研磨法和低频超声法回归方程分别是：CFU=2.35×107×OD600+4.42×105、CFU=3.26×107×OD600+6.89×105。结论低频超声法是一种较好的结核分枝杆菌菌悬液制备方法,结合OD600值测定,可成为一种可靠、快速的结核分枝杆菌定量方法。     

Association between drug, alcohol and tobacco use in adolescents and socio-familiar factors

Substance abuse is the result of interaction between constitutional, environmental and socio-demographic factors. Several studies have been demonstrated that the adolescent substance abuse is a serious and growing problem. The aim of our study is to investigate the association between substance, alcohol and tobacco abuse and socio-familiar factors, to evaluate a substance abuse prevention program which decreases substance use in adolescents. A cross-sectional survey was developed and carried out by involving a group of adolescents, randomly selected from the secondary school of Teramo province. Data were collected by a self-administered questionnaire. The associations between substance use and the determinants taken into account was evaluated by the chi-square test of Fisher exact test or trend test when appropriated. Our data show that the alcohol abuse is more frequent in males (41.1%, p = 0.000), while the nicotine abuse is more frequent in females (46.1%, p = 0.005). However, our results show that the abuse of alcohol, nicotine, heroine, cocaine and ecstasy is associated to lack of stability in the family, respectively: 61.5% (p = 0.001); 61.5% (p = 0.022); 7.7% (p = 0.018); 7.7% (p = 0.010): 7.7% (p = 0.004). The nicotine abuse is higher in adolescents living with a smoker: 50.3% (p = 0.000). Finally, the substance abuse is higher in adolescents having friends that are drug-addicts, in particular: alcohol 49.6% (p = 0.000), nicotine 43.0% (p = 0.000), cannabis 27.9% (p = 0.000). This survey suggests that the knowledge of factors influencing the initiation, combination and cessation of the use of substance is crucial for the prevention of drug use among adolescents.     

A Longitudinal Study of Urinary Phthalate Excretion in 58 Full-Term and 67 Preterm Infants from Birth through 14 Months

Background: Some phthalates have shown antiandrogenic effects in rat offspring. Premature infants may be exposed to high amounts of specific phthalates during hospitalization, and thus are potentially at risk.Objective: We evaluated longitudinal phthalate exposure and metabolism in full-term (FT) and preterm (PT) infants.Methods: Fifty-eight FT and 67 PT (gestational age, 24.7–36.6 weeks) infants were recruited at birth and followed until 14 months (nine times). Urinary concentrations of metabolites of diethyl phthalate (DEP), dibutyl phthalate isomers (DiBP and DnBP), butylbenzyl phthalate (BBzP), di(2-ethylhexyl) phthalate (DEHP), and diisononyl phthalate (DiNP) were measured in 894 samples. Daily intake and a hazard index for antiandrogenic effects were estimated, and excretion patterns of DEHP and DiNP metabolites were analyzed.Results: Metabolites of BBzP, DiNP, and DEHP were 5–50 times higher at day 7 (D7) and month 1 (M1) in PT than in FT infants. Thereafter, metabolite concentrations were similar between the two groups. The estimated hazard index for combined DiBP, DnBP, BBzP, and DEHP exposures 7 days after birth exceeded the antiandrogenic threshold in > 80% of PT and > 30% of FT infants, and after M2, in 30% of all infants. The excretion pattern of DEHP and DiNP metabolites changed with age.Conclusion: Most PT infants and approximately one-third of healthy FT newborns were exposed to phthalates during early life at a potentially harmful level according to the European Food Safety Authority’s recommended limits of daily exposure. Changes in the relative proportions of secondary phthalate metabolites over time were consistent with maturation of infant metabolic pathways during the first year of life. Further research is needed on the health effects of phthalate exposures and the influence of changes in metabolic capacity in neonates and infants.Citation: Frederiksen H, Kuiri-Hänninen T, Main KM, Dunkel L, Sankilampi U. 2014. A longitudinal study of urinary phthalate excretion in 58 full-term and 67 preterm infants from birth through 14 months. Environ Health Perspect 122:998–1005; http://dx.doi.org/10.1289/ehp.1307569     

Postoperative nosocomial endophthalmitis: is perioperative antibiotic prophylaxis advisable? A single centre's experience

Healthcare-associated pneumonia (HCAP) is considered to represent a category of disease distinct from community-acquired pneumonia (CAP). We describe the incidence and characteristics of HCAP compared with CAP in patients hospitalised through the emergency department (ED). Pneumonia diagnosed at the ED of Ghent University Hospital from 1 November 2006 to 31 October 2007 was retrospectively categorised as CAP or HCAP according to the definition of the American Thoracic Society/Infectious Diseases Society of America. We categorised 287 episodes of pneumonia, diagnosed in 269 patients, as CAP [159 (55%)] or HCAP [128 (45%)]. Patients with HCAP were older [75 years (range: 64-83) vs 68 (41-78); P < 0.001], had more comorbidity, and had more severe pneumonia [CURB-65: 2 (1-3) vs 1 (0-2); P < 0.001] in comparison to patients with CAP. Patients with HCAP had more frequently an unfavourable clinical course (27% vs 15%; P < 0.01) and a longer hospital stay (12 days vs 9 days; P<0.001) compared with patients with CAP. In multivariate regression analysis, nursing home residence (odds ratio: 2.96; 95% confidence interval: 1.12-7.84; P = 0.03) but not HCAP was an independent predictor for in-hospital mortality. In conclusion, a high percentage (45%) of patients hospitalised with pneumonia through the ED was classified as HCAP. Classification as HCAP was associated with an unfavourable clinical course. Nursing home residence was an independent predictor for increased mortality.     

不同感染类型的儿童社区获得性肺炎流行病学特征研究

目的 描述不同感染类型的儿童社区获得性肺炎（CAP）在不同性别、年龄、季节间的分布,为儿童CAP正确诊断提供参考依据以及制定合理用药规范奠定基础。方法 共纳入1 155例合格儿童CAP病例,采用间接免疫荧光法（IFA）、被动凝集法、酶联免疫吸附试验（ELISA）法、胶体金法和细菌培养法联合检测常见呼吸道病原体。根据致病微生物类型将儿童CAP分为肺炎支（衣）原体肺炎、病毒性肺炎、细菌性肺炎、混合感染性肺炎和不明原因肺炎。结果 研究对象中男670例（58.0%）,女485例（42.0%）。患儿年龄为1月～14岁,平均年龄为（3.14±2.78）岁。病原学检测阳性数为758（65.6%）。其中,肺炎支（衣）原体肺炎占56.1%,病毒性肺炎占14.9%,细菌性肺炎占12.0%,混合感染性肺炎占17.0%。肺炎支（衣）原体肺炎在2～5岁组最高（49.9%）,而病毒性肺炎和细菌性肺炎均以1岁以下患儿居多;肺炎支（衣）原体肺炎感染率夏秋季高于春冬季（P＜0.05）,而病毒性和细菌性肺炎均是春冬季高于夏秋季（P＜0.05）;肺炎支（衣）原体肺炎在城市儿童中更常见,而病毒性和细菌性肺炎则无明显城乡分布差异（P＜0.05）。结论 肺炎支（衣）原体肺炎在儿童CAP构成中占主要;不同感染类型的儿童CAP流行特征存在差异。     

Associations between three types of maternal bacterial infection and risk of leukemia in the offspring

A case-control study was nested within two maternity cohorts with a total of 7 million years of follow-up for assessment of the role of bacterial infections in childhood leukemia. Offspring of 550,000 mothers in Finland and Iceland were combined to form a joint cohort that was followed for cancer up to age 15 years during 1975-1997 through national cancer registries. For each index mother-case pair, three or four matched control mother-control pairs were identified from population registers. First-trimester serum samples were retrieved from mothers of 341 acute lymphoblastic leukemia cases and 61 other leukemia cases and from 1,212 control mothers. Sera were tested for antibodies to the genus Chlamydia, Helicobacter pylori, and Mycoplasma pneumoniae. Odds ratios and 95% confidence intervals, adjusted for sibship size, were calculated as estimates of relative risk. M. pneumoniae immunoglobulin M appeared to be associated with increased risk (odds ratio (OR) = 1.6), but the association lost statistical significance when the specificity of the immunoglobulin M was considered (OR = 1.5, 95% confidence interval: 0.9, 2.4). In Iceland, H. pylori immunoglobulin G was associated with increased risk of childhood leukemia in offspring (OR = 2.8, 95% confidence interval: 1.1, 6.9). Since H. pylori immunoglobulin G indicates chronic carriage of the microorganism, early colonization of the offspring probably differs between Iceland and Finland, two affluent countries.     

Determinants of malnutrition risk among the older adult community: a secondary analysis of the Health, Wellbeing, and Aging Study (SABE) in Mexico

OBJECTIVE: To determine the psychosocial and health determinants associated with malnutrition risk (MR) among older adults living in the community of Mexico City, Mexico. METHODS: This was a cross-sectional study. Secondary analysis was performed on the data of adults who were 60 or more years of age, living in the metropolitan area of Mexico city, and had participated in the multi-city study on Health, Wellbeing, and Aging in 1999 and 2000. Information on 820 participants was analyzed (mean age 69.7 +/- 7.6 years; 62.9% female). In addition to the MR (dependent variable) that was established through a basic nutrition evaluation, the following variables were analyzed: sociodemographics, body mass index, comorbidity, symptoms of depression, oral health, mental function, functional capability, among others. Independent associations from among the variables and the MR were calculated by univariate and multivariate logistic regression analysis. Odds ratios (OR) and 95% confidence intervals (95%CI) were determined. RESULTS: MR was present in 261 (31.8%) participants. The univariate logistic regression analysis adjusted for possible confounding variables showed that the following variables demonstrated significant and independent associations with MR among the study sample population: not having a pension (adjusted OR = 1.45; 95%CI: 1.01 - 2.38); feeling that one did not have enough money to live on (adjusted OR = 2.52; 95%CI: 1.69 - 3.74); having osteoarthritis (adjusted OR = 2.34; 95%CI: 1.42 - 3.85); having a low body mass index (adjusted OR = 0.89; 95%CI: 0.85 - 0.93); having symptoms of depression (adjusted OR = 5.41; 95%CI: 1.90 - 15.34); eating only once daily (adjusted OR = 12.95; 95%CI: 5.19 - 32.28) or twice daily (adjusted OR = 3.27; 95%CI: 2.18 - 4.9); and having physical difficulty with getting to bed (adjusted OR = 3.25; CI 95%: 1.58 - 6.68), going out alone (adjusted OR = 2.70; CI 95%: 1.54 - 4.73), and using the telephone (adjusted OR = 1.95; CI 95%: 1.10 - 3.43). CONCLUSIONS: There are multiple and various determinants of malnutrition risk. To determine MR, the older adult's financial and social situation must be carefully evaluated along with the more traditional health and anthropometric information.     

Family history and socioeconomic factors as predictors of myocardial infarction, unstable angina and stroke in an Italian population

A comprehensive case-control study was conducted in an Italian region in order to compare the influence of family history of cardiovascular events, socioeconomic factors, social networks, and their joint associations with major risk factors, on the risk, of myocardial infarction (MI), unstable angina (UA) and ischemic stroke (IS). A total of 513 patients with MI, 178 with UA, 237 with IS, and 928 hospitalised controls were recruited. The odds ratio (OR) of MI for two or more relatives with a positive history of MI was 3.6 (95% CI: 1.8–7.3). Family history of MI was predictive for UA (OR = 5.8; 95% CI: 1.2–28.7), but not for IS. A family history of stroke was more associated with the risk of MI than of IS. After adjustment for known risk factors, the OR of MI for more educated people was 2.1 (1.3–3.6) compared with less-educated people. Large family size seemed to be protective for MI. The effect of major risk factors on MI ranged from additive (diabetes) to multiplicative jointly with high education and family history of MI. A family history of stroke increased IS risk threefold jointly with smoking and hyperlipidemia, and eightfold with diabetes. Besides a family history of MI and IS, in this community a higher educational status seems to better identify groups at increased risk of MI. The joint associations have important preventive implications since by identifying high-risk individuals (for MI and IS) a more careful assessment and control of risk factors amenable to intervention may be performed. Participating clinical centres: Atessa: L. Massa, M. Puglfese (Medicina); Avezzano: G. Rabftti, G. Del Bove Orlandi (Medicina); E. Laconi F. Tiburzi (UTIC); Casoli: M. Padula (Medicina); Castel di Sangro: P Caracciolo, V Di Tomasso (Medicina); Chieti: S. Sensi, G. Bitti (Medicina); D. Gambi, S. Viola (Neurologia); A. Rossi, C. Ciglia (UTIC); Gissi: M. Colaneri (Medicina); Giulianova: N. Di Giacinto (UTIC); Guardiagrele: F. Liberatoscioli, M. De Luca (UTIC); Lanciano: V Montagano, B. Di Martino (Neurologia); A. Ottaviano, G. Mastrogiuseppe (UTIC); L'Aquila: E. Marsili, P. Marsili (UTIC); Ortona: G. Giambuzzi (Medicina); Penne: O. Pesa (Medicina); D. Di Gregorio, G. Di Paolo (UTIC); Pescara: B. Carosella, A. Mancini (Medicina); G. Liberti, G. Acerbi (Neurochirurgia); E. D'Annunzio, C. Stoduto (UTIC); Pescina: A. Biocca, G. Barbati (Medicina); Popoli: D. Artese, M. Di Cioccio (Medicina); A. Mobili (UTIC); S. Omero: G. Triozzi, E. Di Biase (Medicina); Sulmona: E. Conti, G. Bonasia (UTIC); Tagliacozzo: G. Appodia (Medicina); Teramo: L. Piccioni (UTIC); Tocco Casauria: M. Zappacosta (Medicina); Vasto: Del Fomo (Medicina); D-Antonini, M. Cipulli (Neurologia); G. Di Marco, E. Bottari (UTIC). UTIC: Unità di Terapia Intensiva Coronarica.PROGETTO 3A (Alimentazione - Aterosclerosi - Abruzzo). Scientific advisory board: F. Capani, F. D'Agostino G. de Gaetano & F. Stante