AT1R、AT2R与原发性高血压相关性研究

肾素～血管紧张素系统（rennin angitensin system,RAS）是由肾素、血管紧张素及其受体构成的重要体液系统,在调节心血管系统的正常生理功能与高血压、心肌肥大、充血性心力衰竭等病理过程中具有重要作用。RAS不仅存在于体液系统,而且在肾脏、心脏、血管与脑组织中也有肾素-血管紧张素系统,协同缓激肽系统调节局部的生理病理过程。近年来各方面对该系统的研究,尤其是其与原发性高血压的关系已引起人们的广泛关注。在众多原发性高血压（essentialhypertension,EH）的候选基因和候选位点中,编码RAS的基因是最有可能的易感基因,也是迄今为止研究最广泛的高血压相关基因。其中血管紧张素Ⅱ（AngⅡ）的有关受体是RAS作用于效应器的关键成分,作为肾素血管紧张素系统最后的介质,血管紧张素Ⅱ必需通过与其受体结合才能发挥生理作用。本文对血管紧张素Ⅱ受体的分子生物学特征、功能及与原发性高血压的相关性做一综述。     

脑卒中患者外周血sIL-6R和sIL-2R水平及相关性分析

目的 :探讨脑卒中患者外周血中SIL 6R和SIL 2R水平的变化及其相关性。方法 :本文对脑卒中患者 (脑出血 30例 ,脑血栓 30例 )外周血可溶性白细胞介素 6受体 (sIL 6R)和白细胞介素 2受体 (sIL 2R)水平进行检测和其相关性进行分析。结果 :脑出血患者外周血sIL 6R和sIL 2R水平高于正常对照组 ,有显著差异 (P <0 0 5 ;P <0 0 1) ;脑血栓患者外周血sIL 6R和sIL 2R水平高于正常对照组 ,有显著差异 (P <0 0 1;P <0 0 1) ;脑出血和脑血栓患者外周血sIL 6R和sIL 2R相关性经统计学处理无差异 ,即sIL 6R和sIL 2R水平不相关。讨论 :sIL 6R在脑缺血区可诱导T、B淋巴细胞分化 ,增强免疫反应 ;sIL 2R可增强IL 6对T细胞增生的诱导作用。因此 ,脑卒中患者机体处于高免疫反应状态 ,这种高免疫反应状态有利于病人的恢复。     

无脾综合征一例报告

病例:患儿,女,2008年12月3日出生。家属发现患儿出生后即有口唇及指(趾)端青紫,哭闹时尤为明显,足掌及手掌皮肤也可见青紫样改变,后在江苏省无锡市儿童医院行心脏彩超提示:先天性心脏病[单心室(右心室)、单心房、     

白介素-2受体监测在肾移植术后的作用

本文简要介绍了IL 2、IL 2R及sIL 2R水平监测在肾移植术后的意义以及抗IL 2R单克隆抗体的发展现状。综述了它们在肾脏移植排异反应的预测、诊断、鉴别及防治中的作用本文简要介绍了IL 2、IL 2R及sIL 2R水平监测在肾移植术后的意义以及抗IL 2R单克隆抗体的发展现状。综述了它们在肾脏移植排异反应的预测、诊断、鉴别及防治中的作用     

Effect of Rear Wheel Suspension on Tilt-in-Space Wheelchair Shock and Vibration Attenuation

Background

Suspension systems are designed to reduce shock and vibration exposure. An aftermarket rear-wheel suspension system is now available for manual tilt-in-space wheelchairs.

Demonstrating Construct Validity of the American Board of Physical Medicine and Rehabilitation Part I Examination: An Analysis of Dimensionality

Background

Ideally, high-stakes examinations assess 1 dimension of medical knowledge to produce precise estimates of a candidate’s performance. It has not been reported whether the American Board of Physical Medicine and Rehabilitation Part 1 Certification Examination (ABPMR-CE-1) is unidimensional or not.

LRRK2基因的基本特征及各种常见突变位点与帕金森病的关系

帕金森病（Parkinson’s disease,PD）是一种常见的中枢神经系统退行性疾病,主要以静止性震颤、肌强直、动作迟缓和姿势反射障碍为主要临床表现,其发病机制尚不清楚。LRRK2基因（leucine—rich repeat kinase 2 gene,PARK8;OMIM607060）是新近在家族聚集性常染色体显性遗传的PD患者中发现的致病基因,     

中国新疆维吾尔族人群基质金属蛋白酶9基因R279Q多态性与冠状动脉粥样硬化性心脏病的关联

背景:基质金属蛋白酶9 与动脉粥样硬化的发生发展密切相关,基质金属蛋白酶9 基因多态性可能与冠状动脉粥样硬化性心脏病(冠心病)遗传易感性相关.目的:探讨新疆维吾尔族人群基质金属蛋白酶9 基因6 号外显子上R279Q(rs17576)多态性与冠状动脉粥样硬化性心脏病发病的相关性.方法:采用聚合酶链反应-限制性片段长度多态性方法对362 例维吾尔族冠心病患者和413 例冠脉造影阴性者基质金属蛋白酶9 基因R279Q 多态性进行分析,比较两组间基质金属蛋白酶9 基因多态性频率分布的差异.结果与结论:冠状动脉粥样硬化性心脏病组和对照组RR,RQ 和QQ 基因型频率分别为34.3%,42.8%,22.9%和37.8%,43.3%,18.9%.冠状动脉粥样硬化性心脏病组RQ+QQ 基因型和Q 等位基因频率(65.7%,44.3%)与对照组(62.2%,40.6%)频率近似( χ2=1.04,P=0.31; χ2=2.26,P=0.13).R279Q 多态性分布在冠状动脉粥样硬化性心脏病组不同冠脉病变支数亚组中也无显著性差异( χ2=0.61,P=0.74).提示基质金属蛋白酶9 基因R279Q 多态性与新疆维吾尔族人群冠状动脉粥样硬化性心脏病的发生、发展无明显相关性.     

不同疗法对晚期结肠癌患者癌性腹水和血清中sIL-2R的影响

目的探讨不同化疗方法对晚期结肠癌患者癌性腹水和血清中sIL-2R的影响.方法对晚期结肠癌伴癌性腹水患者160例中80例采用腹腔免疫化疗[肿瘤坏死因子(TNFα)+卡铂腹腔注射],40例采用卡铂腹腔化疗,40例采用卡铂静脉化疗.用双抗体夹心ELISA法检测患者治疗前及治疗后一周腹水和血清中可溶性白细胞介素-2受体(sIL-2R)水平.结果腹腔免疫化疗组患者癌性腹水消退的有效率达75%,治疗后sIL-2R水平较治疗前显著下降(P<0.01).结论腹腔免疫化疗疗效显著,并可显著降低结肠癌患者免疫抑制因子sIL-2R水平.     

3.0T MR黑血技术在纤维蛋白靶向对比剂动态评价兔颈动脉血栓中的应用

目的 探讨和优化纤维蛋白靶向对比剂(EP-2104R)对兔颈动脉急性和亚急性血栓进行3.0T MR的检查序列和检查时间. 方法 以颈动脉外部损伤和阻塞法制备兔颈动脉内血栓形成模型,分为急性期组(6 h)和亚急性期(6 d)组.分别于EP-2104R注入前及注入后不同时间点行MR黑血技术扫描,观察血栓形态及信号等特点,计算血栓对比噪声比(CNR),并与组织病理学对比. 结果 在T1WI双翻转快速自旋回波压脂序列(T1WI-DIR压脂序列),EP-2104R准确地显示所有颈动脉血栓,呈高信号亮点,信号均匀,边缘清楚,优于非对比增强MRI (P<0.01).亚急性期血栓的CNR明显高于急性期血栓的CNR (P<0.001).血栓CNR在2～3 h达到高峰. 结论 在兔颈动脉血栓形成模型,于T1WI-DIR压脂序列,EP-2104R可以准确显示活体内急性期及亚急性期血栓,优于非对比增强MRI;对活体内不同时期血栓的理想检查时间窗为注射EP-2104R后2～3 h.     

Neuromedin U: physiology, pharmacology and therapeutic potential

Neuromedin U (NmU), a multifunctional neuropeptide, belongs to a family of neuropeptides, the neuromedins. It is ubiquitously distributed with highest levels found in the gastrointestinal tract and pituitary. The conservation of structural elements of NmU across species, the widespread distribution of NmU and its receptors throughout the body point to a fundamental role in key physiological processes. Two G protein coupled receptors for NmU have been cloned NmU R1 and NmU R2. NmU R1 is expressed pre-dominantly in the periphery especially the gastrointestinal tract whereas NmU R2 is expressed pre-dominantly in the central nervous system. Current evidence suggests a role of NmU in pain, in regulation of feeding and energy homeostasis, stress, cancer, immune mediated inflammatory diseases like asthma, inflammatory diseases, maintaining the biological clock, in the regulation of smooth muscle contraction in the gastrointestinal and genitourinary tract, and in the control of blood flow and blood pressure. With the development of drugs selectively acting on receptors and knockout animal models, exact pathophysiological roles of NmU will become clearer.     

食管癌患者IL-2R的表达和血清SIL-2R的研究

目的观察食管癌组织ＩＬ－２Ｒ的表达和患者血清ＳＩＬ－２Ｒ的变化，探讨ＩＬ－２Ｒ和ＳＩＬ－２Ｒ与食管癌发生、发展的关系。方法采用双抗体夹心ＥＬＩＳＡ法对４２例食管癌患者血清可溶性白细胞介素２受体（ＳＩＬ－２Ｒ）水平进行测定，同时应用即用型ＳＡＢＣ免疫组织化学方法观察ＩＬ－２Ｒ在食管癌组织的表达情况。结果食管癌患者血清ＳＩＬ－２Ｒ水平显著高于正常对照组（Ｐ＜０．０５），并与临床分期有关；ＩＬ－２Ｒ在食管癌组织中阳性表达率５２．４％（２２／４２），显著高于正常食管粘膜组（Ｐ＜０．０５），并与临床分期有关。结论ＩＬ－２Ｒ表达和血清ＳＩＬ－２Ｒ水平可作为临床诊断和判断患者预后的重要参考指标。     

Rate dependent far-field R wave sensing in an atrial tachyarrhythmia therapy device

A case is reported of far-field R wave (FFRW) oversensing resulting in inappropriate atrial tachycardia (AT) detection by a dual chamber pacemaker incorporating atrial autoadjusting sensitivity (AAS). FFRW oversensing occurred during periods of functional atrial undersensing (FAU) with PR interval prolongation. Limitations of the pacemaker's ability to reject FFRWs and programming considerations for addressing this unique behavior are discussed.     

康复疗养对高原官兵超氧化物歧化酶影响的研究

目的研究康复疗养对高原官兵超氧化物歧化酶(SOD)的影响。方法将刚下4 000 m以上高原赴临潼疗养院的30名高原官兵分为正常疗养组和疗养+高压氧组,分别于入院时、疗养15 d检测SOD的变化。结果高原官兵入院后经过疗养因子等综合措施,疗养15 d检测SOD明显降低,正常疗养组与疗养+高压氧组差异无统计学意义(P0.05)。结论疗养15 d对高原官兵SOD的调节有一定的影响,对官兵身心健康有很好的调节作用。     

R软件R2WinBUGS程序包在网状Meta分析中的应用

R软件是一个自由、免费及开放的统计软件,并具有强大的绘图功能,但其处理网状Meta分析数据的代码命令较为复杂,因此易错且不易掌握.WinBUGS软件是基于贝叶斯理论而开发的一款统计软件,具有强大的数据处理功能,在处理网状Meta分析数据时,代码编写易懂且操作方便,但对结果的绘图功能欠佳.为充分发挥R软件与WinBUGS软件各自的优势,开发者在R软件的基础上研发了R2WinBUGS程序包,构建了R软件与WinBUGS软件之间的“桥梁”,使网状Meta分析的过程和结果处理方便快捷又美观.本文以实例操作的方式展示使用R软件R2WinBUGS程序包进行网状Meta分析的过程.     

应用间断流动式血细胞分离机采集外周血造血干细胞的效果分析

目的:为寻找采集外周血造血干细胞更合适的手段,探讨间断流动式血细胞分离机采集外周血造血干细胞的效果。方法:选择中山大学第五附属医院血液风湿科2004-2006年12例行外周血造血干细胞移植住院患者。①实验对象:供者5例,男1例,女4例,一般状况良好,与患者关系为同胞妹妹3例,同胞弟弟1例,同胞姐姐1例;患者7例,男3例,女4例,23～62岁,7例为自体移植,5例为异基因移植。血液系统疾病患者9例(非霍奇淋巴瘤3例,急性淋巴细胞白血病2例,急性髓细胞白血病2例,慢性粒细胞白血病1例,骨髓增生异常综合征1例);自身免疫性疾病3例(重型系统性红斑狼疮2例,难治复发性类风湿关节炎合并干燥综合征1例)。②实验过程:每位供/患者均知情同意并签署知情同意书。对于自体患者,根据患者的疾病类型采取不同的干细胞动员化疗方案,联合化疗后7～10d,待白细胞下降至最低点,一般为≤1.0×109 L-1时,给予粒细胞集落刺激因子5μg/kg皮下注射,白细胞升至3.0×109 L-1时开始采集;对于allo-PBSCT供者直接给予粒细胞集落刺激因子5μg/kg,皮下注射,1次/d,共5d,同时应用流式细胞仪检测CD34 细胞数,至白细胞升至≥20.0×109 L-1或当CD34 细胞升高>20个/μL时采用增强型多功能血细胞分离机PBSC程序卡采集健康供者和患者外周血造血干细胞。③实验评估:分析采集效率、血液学参数、不良事件发生率、以及供、受者ABO血型不合的患者回输干细胞溶血反应等情况。结果:12例供者、患者均进入结果分析。①共进行了24次采集,其中1次采集1例,2次采集7例,3次采集3例,平均循环次数(25±5)次,采集时间(228±32)min,处理血量(7234±1205)mL,复方枸橼酸钠溶液用量为(623±96)mL,干细胞收集量为(102±21)mL,CD34 细胞采集效率为(54.3±30.7)%,细胞计数示白细胞为(156±34)×109 L-1,单个核细胞为(79.7±13.2)×109 L-1,流式细胞仪检测CD34 细胞为(10.30±4.38)×106/kg受者体质量。②不良反应轻微,24次采集过程中出现不良反应6次均为枸橼酸盐所致低钙血症反应。血红蛋白和血小板与采集前相比分别下降9.36%和11.10%。供、受者ABO血型不合的3例患者在输注造血干细胞悬液后均未出现溶血反应。③12例均获造血功能重建,无移植相关死亡。结论:用间断流动式血细胞分离机采集外周血造血干细胞效果良好,不良反应轻微,能有效的减少被采集者红细胞、血小板的丢失,对供、受者ABO血型不合者不需去除造血干细胞悬液中的红细胞,值得临床应用。     

Frequencies of circulating CD4+CD25+CD127low cells in atopics are altered by bronchial allergen challenge

Background Recent studies in rodents revealed that regulatory T cells (T reg cells) with CD4+CD25+ phenotype can exert suppressive effects on experimentally‐induced allergic airway inflammation and airway hyper‐reactivity. It is unclear however, whether modulations of bronchoconstriction responses in human subjects might be related to T reg cells. We report here for the first time the changes in frequency of circulating lymphocytes with putative T reg cell phenotype (CD4+CD25+CD127low) in relation to bronchoconstriction phenotype following an intrabronchial allergen challenge. Material and methods Thirty‐one house dust mite sensitive patients were challenged with Dermatophagoides pteronyssinus extract (Dp). Eleven isolated early responders (IER) were compared with nine dual (early and late) responders (DR) and to 11 non‐responders (NR). Frequencies of peripheral blood CD4+CD25+CD127low lymphocytes were assessed in all groups of patients by using three‐parameter flow cytometry before, and six and 24 h, after allergen inhalation. Results At baseline, frequencies of CD4+CD25+CD127low lymphocytes were not statistically different among NR, IER and DR. When all individuals were analyzed together, a statistically significant decrease in frequency of CD4+CD25+CD127low lymphocytes was observed 6 h after the bronchial challenge. Interestingly, such a pattern was found consistently only in NR, while IER and DR displayed varying responses resulting in a trend similar to that of NR. Twenty‐four hours after the bronchial challenge, frequencies of CD4+CD25+CD127low lymphocytes in all groups tended to return to baseline values. Conclusions Our data indicate that bronchial allergen inhalation in sensitive patients (predominantly in those who did not develop significant bronchoconstriction) is associated with a decrease of proportion of peripheral lymphocytes with regulatory T cell phenotype.     

Mutation R273H confers p53 a stimulating effect on the IGF-1R-AKT pathway via miR-30a suppression in breast cancer

p53 is the most highly mutated tumor suppressor in human malignancies. A wide array of p53 mutations has been revealed to play pivotal roles during cancer progression, which abolish anti-tumor functions of wild type p53 but also elicit tumorigenic effects by activating a diverse subset of downstream molecules. R273H mutation of p53 has been closely implicated in human cancer. Here we report miR-30a as a novel downstream target of p53 R273H mutant, which binds to the promoter region to repress miR-30a expression. Consequently, p53 R273H mutant enhances the migratory capabilities of tumor cells that are compromised by exogenous miR-30a over-expression. Our further investigation indicates that p53 R273H mutation unleashes the inhibition effect of miR-30a on IGF-1R expression, thus leading to elevated activation of IGF-1R-AKT signaling cascade in tumor cells.     

Diasys R/S 2003尿沉渣定量分析工作站在筛检尿路感染的临床应用

尿液标本的细菌培养检查对于尿路系统感染的诊断有很重要的临床价值,但是常规细菌培养需要3天才出检测结果,且80%以上为阴性。本研究利用D iasys R/S 2003对离心尿的沉淀物进行细菌计数,筛检出细菌培养预计为阴性结果的标本,探讨这种方法筛检尿路感染的可行性。现报告如下。1材     

Cas12a variants designed for lower genome-wide off-target effect through stringent PAM recognition

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