Experience of endometrial stromal sarcoma with fertility conservation treatment

The paper introduced the experience of a case of recurrence of endometrial stromal sarcoma with fertility conservation treatment after full-term delivery. A 24-year-old unmarried patient who, with her increasing amount of menstruation in the late six months, had a mass in her lower-abdominal which was confirmed by B-ultrasonic diagnosis as myometrium mixed mass, was given an enucleation of uterus tumor. The pathological examination of tumor sample revealed it as lowgrade endometrial stromal sarcoma （LESS）. And the patient underwent 6 cycles of chemotherapy over 8 months before she got pregnant and had a full-term delivery. But in 34 months after delivery, the disease recurred with metastatic of pelvic and peritoneal cavity and the patient had to receive comprehensive therapy. Conclusion： The unmarried young patient with stage I low-grade endometrial stromal sarcoma has the chance to preserve uterus for her pregnancy and delivery. But the using of cesarean needs do staging operation and prognosis evaluation.     

Peripheral primitive neuroectodermal tumors： a rare case report

We aimed to explore the diagnosis and treatment of peripheral primitive neuroectodermal tumors （pPNETs）. We retrospectively analyzed the diagnosis and treatment process of a patient who was diagnosed with pPNETs by pathology. This case was a man with soft masses arising from the left chest wall near the armpit and left supraclavicular of a 47-year-old man. The patient mainly presented with the masses which increasing gradually with obvious pain. Needle biopsy showed that they were both metastatic adenocaroinoma. Ultrasonography B revealed blood flow of these two low density placeholders can be seen in the signal, not oppression axillary and vein. Radical resection of the masses were performed. Histopathologic study and immunohistochemistry （IHC） confirmed the masses to be peripheral primitive neuroectodermal tumors, pPNETs is a rare malignant small round cell tumor. CT and MRI examination can estimate the resectability of the tumor; Ultrasound B can make sure its inside blood supply and the positional relationship between the mass and the surrounding vasculature. The diagnosis of pPNETs is based primarily on histopathologic study and IHC, especially those with the characteristics of the Homer-Wright and neuroendocrine markers. Radical resection of the tumor is the most effective therapeutic method. The effect of adjuvant chemo-radiation is worth affirmation. Autologous stem cell rescue besides adjuvant chemotherapy has been associated with prolonged survival.     

Clinicopathological and immunohistochemical features of pilomyxoid astrocytoma： a report of six cases

Objective： The aim of this study was to study the clinicopathological and immunohistochemical features of pilo- myxoid astrocytoma （PMA）. Methods： The clinical and pathologic features in six cases of PMA were analyzed. Immunohisto- chemical staining for glial fibrillary acidic protein （GFAP）, synaptophysin （Syn）, Chromogranin A （CgA）, cytokeratin （AEI/AE3）, epithelial membrane antigen （EMA） and Ki67 was performed on paraffin-embedded sections. Results： Among the six cases, five occurred in female patients, one was male, the age at diagnosis ranged from 2 to 15 years. Four cases were located in the hypothalamic area and optic pathway, one case in the third ventricle, and one case in left parietal lobe. On imaging, PMAs often appears as well-circumscribed mass. Microscopically, the tumor was composed of monomorphous bipolar （piloid） cells setting in a prominent myxoid background with an angiocentric radiating growth pattern in some areas. PMA lacked biphasic pattern, Rosenthal fibers and eosinophilic granular bodies which were usually typical in a classic pilocytic astrocytoma （PA）. Immunohistochemcal study showed that the tumor cells were diffusely positive for GFAP. Syn positive staining was observed in one case. The Ki67 labeling index measured less than 5%. Conclusion： PMA is a distinct aggressive variant of pilocytic astrocytoma with special histological and immunohistochemical features. It is typically a rare tumor of early childhood. Im- munohistochemical staining for GFAP and Syn is helpful in differential diagnosis.     

Right atrial angiosarcoma and electrocardiogramgated cardiac computed tomography： a case report

Primary cardiac tumors are quite rare and most of these tumors are benign. In this report, a patient presented with chest distress and shortness of breath after activity. Echocardiography of other hospital showed a hyperechoic right atrial mass. Electrocardiogram-gated cardiac computed tomography （ECG-Gated CT） of our hospital provided accurate information about the site and extent of the tumor, and the involvement of neighboring structures, even about the malignant nature of the lesion. The pathological study indicated angiosarcoma. The role of ECG-Gated CT in the assessment of cardiac masses and tumors was discussed. Cardiac tumors are extremely rare and can be divided into benign and malignant lesions. Myxomas are the most common type of cardiac benign tumor, while angiosarcomas are the most common type of cardiac malignant tumor. Imaging studies play an important role in the diagnosis of cardiac angiosarcomas. Echocardiogram, computed tomography （CT） and magnetic resonance imaging （MRI） are the most common imaging studies for tumors evaluation. However, the precise tumor location is often difficult to evaluate precisely on the basis of two-dimensional source images. We conducted ECG-gated cardiac CT examination with 3D reconstruction for preoperative assessment in a patient with a angiosarcoma arising in the right atrium.     

Primary choriocarcinoma of uterine cervix treated by uterine artery drug pouring and embolism： one case report

Primary choriocarcinoma of the uterine cervix （PCC） is an extremely rare disease. The conventional treatment of PCC is a combination of hysterectomy and chemotherapy. We present one rare case proved by cervical biopsy. The patient was an 36-year-old Chinese woman with irregular vaginal bleeding for 60 days. A cervical tumoral mass was seen in the pel- vic examination and biopsy revealed active hyperplasia of trophoblastic cell. Because of massive vaginal haemorrhage, the patient accepted uterine artery drug pouring and embolism emergently. This management had gained a satisfactory effect. Thus, Uterine artery drug pouring and embolism is one new and effective weapon for PCC, which can preserve the patient＇s productive ability.     

Reconstruction of composite defects of the nose following tumor resection - a single author＇s experience and evaluation of the outcome

Objective： The aim of the study was to present our experience in reconstruction of complex defects of the nose. Methods： Fourteen patients presenting with large composite defects of the nose were anatomically reconstructed after full tumor clearance of a skin cancer. The aesthetic outcome was assessed subjectively and objectively while the functional outcome was only assessed subjectively in 13 patients. Results： Basal cell carcinoma （BCC）, represented the tumor excised in 10 patients while the remaining 4 patients suffered from squamous cell carcinoma （SCC）. One patient died of extensive local recurrence of SCC after 6 months. The commonest flap used for inner lining was the septal flap while the forehead paramedian flap provided the external coverage for the majority of patients. All flaps survived completely except in one patient who developed distal paramedian forehead flap necrosis. Two patients developed multiple abscesses and sinuses discharg- ing parts of cartilage grafts through the flaps＇ skin with one patient suffering from total extrusion of the costal cartilage grafts. Two other patients suffered from severe nostril stenosis. All patients had variable degree of difficulty in airway passage, while most patients were satisfied with the total nasal appearance. The objective assessment of the overall appearance of the nose scored less than the subjective satisfaction. Conclusion： Reconstruction of complex nasal defects has a high learning curve. Intranasal flaps are usually of limited size and nostril asymmetry is likely to occur. Local or regional skin flaps if available are still considered a good choice for inner as well as outer lining.     

Expression levels of CXCR4 and VEGF correlate with blood-borne metastatic progression and outcome in patients with osteosarcoma

Objective： We determine whether chemokine receptor CXCR4 and vascular endothelial growth factor （VEGF） expression related to the metastasis and survival outcome of patients with osteosarcoma. Methods： Tissue microarray （TMA） was used to detect the expression of CXCR4 and VEGF in 56 osteosarcoma patient samples. Two-year follow-up was performed to observe the metastatic behavior and overall survival of osteosarcoma patients. Results： There was a significant correlation between the expression levels of CXCR4 and VEGF in 56 osteosarcoma patient samples （P = 0.002）. Univariate analysis revealed the expression of CXCR4 and VEGF was not associated with age, gender and the level of ALP but associated with clinical stage. Conclusion： These data raises the possibility that VEGF could regulate the levels of CXCR4 to promote the migration of tumor cells to target organs. CXCR4 and VEGF expression are highly correlated with metastatic progression in patients with osteosarcoma and their immunohistochemical expression have predictive value for the metastatic development.     

Value of Multi-slice spiral CT in diagnosis of malignant gastrointestinal stromal tumors

Objective： The aim of this study was to investigate the value of multi-slice spiral CT （MSCT） in the diagnosis of malignant gastrointestinal stromal tumors （GISTs）. Methods： Twenty-seven cases of MSCT images of malignant GIST proved by surgery and pathology were retrospectively analyzed. Both plain and enhanced CT scan was performed and multiplanar reconstruction was made in all cases. Results： The lesions originated from the stomach （n = 11）, small intestine （n = 9）, colon （n = 4）, rectum （n = 1）, and mesentery （n = 2）. The transverse diameters of mass were 4.2-22 cm, the edges clearly （n = 12）, unclearly （n = 15）. The mass were mainly irregular in shape Iobulated （n = 19）. The lesions were mainly heterogeneity on plain scan, moderate to marked enhancement in arterial phase and durative enhanced in venous phase. Cystic necrosis were observed in all the lesions, 9 cases were cystic and solid mixed mass. Hepatic metastases （n = 4）, pulmonary metastasis （n = 1）, lymphatic metastasis （n = 2） were detected. The accuracy rate of MSCT diagnosis for location and pathologic features of GISTs were 85.2% （23/27） and 77.8% （21/27）. Conclusion： Two-phase MSCT examination and axial images combined with multiplanar reconstruction images have important value for diagnosis of malignant GIST.     

Evaluation of the effect of neoadjuvant chemotherapy on tumor and axillary lymph nodes in locally advanced breast cancer： a study of 50 patients

Objective： The purpose of the study was to correlate between effect of pre-neoadjuvant chemotherapy （NACT） and post-NACT clinical, sonographic and pathologic features of the tumor and axillary lymph nodes （ALNs） and to raise the possibility of applying the concept of sentinel lymph node biopsy （SLNB） in patients with initially positive ALNs before NACT. Methods： A prospective study of 50 female patients with locally advanced breast cancer （LABC） with clinically palpable.and cytologically （under ultrasonographic guidance） positive ALNs. All patients received NACT and then referred for ultrasono- graphic assessment of the axilla regarding any detectable sonographic criteria of metastatic deposits in ALNs as well as the tumor size in relation to its prechemotherapy size, All patients were then subjected either to modified radical mastectomy or breast conserving surgery. The clinical, sonographic and pathological response of the tumor and the ALNs were documented, classified and correlated with each other. Results： Patients＇ mean age was 47.7±9.1 years. The mean clinical tumor size was 6.7 ± 1.4 cm; stage IliA that was presented in 32 patients （64%） and IIIB was presented in 18 patients （36%）. Chemotherapy was given for a median of 4 cycles, there was reduction of the mean clinical tumor size from 6.7 ± 1.4 cm to 4.3 ± 2.7 cm （P 〈 0.001）. Clinical response was complete in 5 （10%） tumors, complete pathological tumor response （post-neoadjuvant） was detected in 6 （16%） of patients. Complete clinical nodal response （post-neoadjuvant） in 23 （46%） axillae, on sonographic assessment of the axilla, response was complete in 17 （34%） axillae. Complete pathological nodal response occurred in 16 （32%） axillae. Out of 17 axillae that showed complete sonographic response 11 axillae showed complete pathological nodal response （P 〈 0.001）. Conclusion： Formal axillary lymph node dissection can be avoided and replaced by SLNB post NACT in patients with L     

Primary extraosseous Ewing＇s sarcoma of the lung in a 36-year-old female patient： clinical,imaging, and pathologic findings

Primary extraosseous Ewing＇s sarcoma is a very rare clinical entity. In the report, we present the case of a 36- year-old female patient who underwent resection of lung mass and was diagnosed as having Ewing＇s sarcoma by pathologi- cal examination. The clinical, imaging, and pathologic features of the case are described and discussed.     

Clinical features and prognosis of obese breast cancer patients： a retrospective study

Objective： The aim of our study was to investigate the prognosis of obese breast cancer patients. Methods： This study was conducted on a total of 317 breast cancer patients who were histopathologically and clinically diagnosed at the General Hospital of Shenyang Military Region （China） from 2004 to 2006. Clinical data including height, weight, age at diagnosis, tumor size, lymph node status, menopausal status, family history of cancer and hormone receptor status were col- lected. Log-rank test was performed to compare the disease free survival （DFS） and overall survival （OS）. Cox proportional hazards regression analysis was conducted to make multivariate analysis. The Chi square test was used to compare the clinical features among normal weight group, overweight group, and obese group. Results： Obesity was an independent prognostic factor for DFS （P = 0.022） and OS （P = 0.032） in breast cancer patients. In the stratified analysis based on the hormone receptor status, obesity was independently associated with OS in patients with negative ER/PR （P = 0.002）, but such association was not observed in patients with positive hormone receptors. Obesity was also associated with lymph node status （P = 0.001） and smoking （P = 0.009）. Conclusion： Obesity is associated with poor DFS and OS in patients with breast cancer. Therefore, maintaining normal weight may benefit breast cancer patients.     

Prognostic value of cancer stem cell markers CD133, ALDH1 and nuclear β-catenin in colon cancer

Objective： Colon cancer is one of the most common human malignancies. Cancer stem cells （CSCs）, despite being only a small subset of cancer cells, have the capability to self-renew and sustain the tumor. They also have the ability to proliferate. Multiple CSCs-associated markers have been identified in colon cancer including CD133, ALDH1 and β-catenin. The aim of the work was to study the prognostic value of CSCs markers （CD133, ALDH 1 and β-catenin）, as well as their rela- tionship to clinicopathological features of colon cancer. Methods： CD133, ALDH1 and β-catenin proteins expression was as- sessed immunohistochemically in a series of colon cancers and their prognostic significance was evaluated. Results： CD133 expression showed significant relationship to tumor stage and lymph node metastasis （P-value 0.004 ＆ 〈 0.001 respectively）, and near significant relationship to liver metastasis （P-value 0.092）. ALDH1 was significantly associated with tumor grade, stage and nodal metastasis （P-value 0.021,0.001 and 0.026 respectively）, but its relationship to liver metastasis was near sig- nificant （P-value 0.068）. Nuclear β-catenin was significantly related to tumor grade, stage, nodal and liver metastasis （P-value 0.001, 〈 0.001, 〈 0.001 and 0.008 respectively）. Overall survival （OS） was associated inversely with CD133, ALDH1 positivity, and directly with nuclear 13-catenin posiUvity （P-value 〈 0.001,0.0001 and 〈 0.001 respectively）. Also recurrence free survival （RFS） was associated inversely with CD133, ALDH1 and directly with nuclearβ-catenin positivity （P-value 0.0001,0.001 and 〈 0.001 respectively）. Conclusion： CD133, ALDH1 and β-catenin expressions of tumor cells have significant impact upon malignant progression of colon cancer and thus patient survival and tumor recurrence. Hence they can be used to predict outcome of colon cancer patients.     

Biomechanical evaluation of lumbosacral reconstruction after subtotal sacrectomy： A three-dimensional finite element analysis

Objective： The aim of this study was to investigate the biomechanical property of lumbosacral reconstruction after subtotal sacrectomy. Methods： Three three-dimensional finite element models of lumbosacral region were established： （1） An intact model （INT）; （2） A defective model （DEF） on which subtotal sacrectomy was performed cephalad to the $1 foramina; （3） A reconstructed model （REC）. These models were validated by compared with literature. Upright posture was stimulated under a compression load of 925N. A finite element analysis was performed to account for the displacement and stress on the models. The REC model was calculated twice, with the material property of reconstruction instrument set as titanium and stainless steel, respectively. Results： The displacements of anchor point on the L3 vertebrae in INT, DEF and REC model were 6.63 mm, 10.62 mm, 4.29 mm （titanium） and 3.86 mm （stainless steel）, respectively. The stress distribution of the instrument in REC model showed excessively concentration on caudal spinal rod, which may cause rod failure between spine and ilia. The maximum von Mise stress of stainless steel instrument was higher than that of titanium instruments （992 MPa vs 655 MPa）, and the value of stress of anchor point around sacroiliac joint in REC model were 26.4 MPa with titanium instruments and 23.9 MPa with stainless steel instruments. Conclusion： Lumbosacral reconstruction can significantly increase the stiffness of spino-pelvis of the patient who underwent subtotal sacrectomy. However, the rod between L5 and ilia is the weakest region of all the instruments. It is suggested that the bending of rod should be conducted carefully and smoothly to avoid significant stress concentration so as to reduce the risk of rod failure. And stainless steel instrument has higher maximum stress and significantly greater stress shielding effect than titanium instrument, which means stainless steel instruments are of higher risk of rod failure and less favorable     

Clinical results and histological changes following preoperative interventional treatment of the aggressive subtype of endometrial cancer

Objective： The aim of the study was to evaluate the clinical value of preoperative intra-arterial infusion chemotherapy and embolization in treating the aggressive subtype of endometrial carcinoma with hysterectomy. Methods： Fifteen cases of endometrial carcinoma were performed intra-arterial chemotherapy and embolization before operation with carboplatin or cisplatin, epirubicin or ADM, and all the cases were performed the arterial chemoembolization with KMG or gel-foam particles mixed with 1/3 total drug dose after 2/3 total drug dose perfusion through the bilateral feeding arteries. Of 15 cases, there were 5 cases with uterine papillary serous carcinoma, 3 cases with endometrial clear cell carcinoma, and 7 cases with endometrial adenosquamous carcinoma. Results： Fifteen cases of endometrial carcinoma were performed operations after 3-4 weeks of intra-arterial chemotherapy and embolization. In these cases, 3 were found mass necrosis and lymphocyte cells infiltration in the tumor tissues but no carcinoma cells, which was noted as histological complete remission （HCR）. After intra-arterial chemotherapy and embolization 3-4 weeks, the expression of proliferating cell nuclear antigen （PCNA） was obviously reduced （P 〈 0.001）. Conclusion： The preoperative intra-arterial chemotherapy and embolization can improve the operability of resection in patients with aggressive subtype of endometrial carcinoma, reduce the expression of PCNA, adjust malignancy of endometrial carcinoma, and improve prognosis.     

Neoadjuvant intra-arterial infusion chemotherapy followed by surgery in patients with locally advanced cervical cancer

Objective： The aim of this study was to investigate the role of preoperative neoadjuvant intra-arterial infusion chemotherapy （NAIC） in treating locally advanced cervical caner. Methods： Nineteen locally advanced cervical cancer （LACC） patients from November 2003 to November 2005 were analyzed retrospectively. NAIC was administrated 2 courses every 2 weeks using a combination of 30 mg/m^2 bleomycin and 50 mg/m^2 cisplatin via bilateral femur artedes. The response to NAIC was assessed by pelvic examination and imaging diagnostics and histological analysis. Two weeks after NAIC radical hysterectomy with pelvic lymphadenectomy was performed. Results： Radical hysterectomy with pelvic lymphadenectomy were performed in 18 patients successfully. The mean tumor reduction rate was 73.04%. The overall clinical response rate of NAIC was 84.2% with 2 complete responses and 16 partial responses. Only 1 nonresponder. Six of 7 cases who had parametrial infiltration had a absence after chemotherapy, no significant change was observed in 1 case who followed by radiotherapy. Multivariate logistic regression analysis indicated that tumor volume prior to treatment was determining factor affecting the efficacy of NAIC in LACC. Conclusion： pre-operative NAIC inhibited the growth of LACC, minimized the size, eliminate effectively the pathologic dsk factors in the pelvic cavity, to improve the operability in cervical cancer patients with stage lib or above, considered inoperable.     

The value of bone scintigraphy on the determination of the full extent of tumor involvement in jaw bones

Objective： To prospectively investigate the value of bone scintigraphy on determining the full extent of tumor involvement in jaw bones and to assess the presence of metastases. Methods： This study had local ethical committee approval, and all patients gave written informed consent. Thirty seven consecutive patients with primary malignant tumor in jaw bones were recruited for the study. Bone scintigraphy was performed in all patients before surgery to measure the full extent of bony involvement, which was compared with histologic findings. Results： Whole body scan revealed one case with multiple bony metastases. Resection specimens of 36 bone neoplasms were pathologically analyzed to identify type and size of each tumor. The lengths of the tumor involvement in jaw bones defined by bone scintigraphy and pathology were 5.62 ± 1.58 cm, 4.48 ± 1.57 cm, respectively （P 〈 0.05）. The tumor negative margins from removed specimens according to bone scintigraphy were pathologically confirmed. With histologic findings as the standard of reference, the accuracy of bone scintigraphy was 100% （36 of 36 patients） in determining the full extent of tumor involvement in jaw bones. Conclusion： Bone scintigraphy tends to offer specific guidelines in determining the appropriate extent of bone resection while entirely clearing the tumor cells and preserving functions whenever possible and in establishing the bony metastases.     

Epithelioid haemangioendothelioma of the lung： one case report with positive pulmonary hilar lymph nodes

Pulmonary epithelioid hemangioendothelioma （PEH） is a rare tumor that occurs among young women and typically presents as bilateral multiple nodules. In this report, we described an unusual case of PEH presented as a single mass in a 46-year-old man, with 6 positive pulmonary hilar lymph nodes. Histologic findings were compared with conventional PEH, except those features such as round to oval-shaped nodules, hypocellular/cellular peripheral zone, hyaline, myxomatous intercellular stroma and intracellular vacuoles, and we found the patient＇s pulmonary hilar lymph nodes were metastasis and cholesterol crystal were discovered in the focal infarction areas. Immunohistochemically, the tumor cells were strong positive for CD34, CD31, factor VIII related endothelial antigen and vimentin. The patient died 3 and half months after the surgery without receiving any radiotherapy or chemotherapy. This report may contribute to the data on clinical and histological finding of this rare tumour.     

Autograft-prosthesis composite for aggressive giant cell tumor of bone around knee

Objective： The aim of the research was to study the clinical feasibility of autograft-prosthesis composite for aggressive giant call tumor of bone around knee. Methods： Seven patients （5 males and 2 females, the mean age of 30.5 years old） with giant cell tumor of bone around knee underwent tumor resection and reconstructed with autograft-prosthesis composites since January 2006. Five lesions located at the distal femur and 2 at the proximal tibia. There were 3 patients with primary tumor and 4 with recurrent. Three patients with pathological fracture and all patients were of Campanacci Ⅲ. Results： All patients were done follow-up from 12 to 36 months. No recurrence, metastasis, and prosthesis loosening were found. The mean healing time between autograft and host bone was 5 months. The mean motion range of affected knee were 90° （70°-110°）. Conclusion： Our data documented the clinical feasibility of autograft-prosthesis composite for giant cell tumor of bone around knee which should be performed tumor resection and reconstructed with prosthesis. The long-term outcomes remain to be further proven.     

Mammographic features of breast cancer： Analysis of 118 cases

Objective： The aim of this retrospective study was to evaluate the mammographic imaging features （markers） on different types of breast cancer and improve early radiological diagnosis of breast cancer. Methods： Analyzed the mammographic images of 118 patients with breast cancer confirmed by surgery and pathology. Among 118 cases, 70 cases were infiltrating ductal carcinoma （59.3%）, 17 cases were ductal carcinoma in situ （DCIS, 14.4%）, 7 cases were mucinous carcinoma （5.9%）, 5 cases were medullary carcinoma （4.2%）, 1 case was tiny micro carcinoma （0.9%） and 18 cases were other types cancer （15.3%）. Results： The mammographic appearance of 118 patients with different breast cancer included： tumor mass （n = 80, 68.8%）, microcalcification （n = 57, 48.3%）, which were subdivided into two groups （a） microcalcification only, 13 cases （13/118, 11%）; （b） combined with other mammographic features： 31 cases with tumor mass （31/118, 26.3%）; 7 cases with architecture distortion （7/118, 5.9%）, 6 cases with focal asymmetric density （6/118, 5.1%）. Six cases appeared as architecture distortion only, 41 cases with abnormal vessel signs （34.7%）. Conclusion： The microcalcifications are the most frequently basal X-ray signs in DCIS. Architecture distortion and focal asymmetric density are special X-ray signs that were easily missed. The abnormal vessels are also important accompaniment signs of breast cancer. The use of coned compression technique is particularly important to improve the radiological diagnosis of breast cancer.     

Establishment of a transplantation tumor model of human osteosarcoma in chick embryo

Objective： We established a transplantation tumor model of human osteosarcoma in chick embryo, studied its morphological and biological characteristics, and observed its dynamic process of angiogenesis induction so that a simple and practical model can be provided for studying osteosarcoma. Methods： Human osteosarcoma cells at different concentrations were inoculated in chorioallantoic membrane （CAM） of chick embryos at different embryonic ages to observe the factors affecting the survival of the transplanted osteosarcoma in chick embryo, growth characteristics of the transplantation tumor, and the morphological characteristics and biological characteristics of the transplantation tumor. Results： The transplantation tumor model of human osteosarcoma in chick embryo was successfully established. It was found that the transplantation tumor was easy to grow and it showed strong angiogenesis-inducing effects. Under the light microscope, the transplantation tumor showed a similar tissue structure to human osteosarcoma. Conclusion： It is feasible to establish a transplantation tumor model of human osteosarcoma in chick embryo. The model can be easily duplicated with a simple operation, which provides a useful animal model for studying osteosarcoma.