骨髓动员与动员后单个核细胞移植治疗糖尿病大鼠后肢缺血的疗效比较

目的 比较骨髓动员与动员后单个核细胞移植对糖尿病大鼠后肢缺血的疗效.方法 糖尿病大鼠后肢缺血模型建立后,将36只大鼠随机分成3组,其中15只于动员后行单个核细胞移植(A组),15只进行骨髓动员(B组),其余6只为空白对照组(C组),不进行骨髓动员和动员后单个核细胞移植.放射免疫法检测大鼠血浆内皮素(endothelin,ET)水平,免疫组织化学法计数毛细血管数目.结果 A组与B组大鼠缺血后肢毛细血管生成的数量均高于C组(P＜0.05),但A组大鼠缺血后肢毛细血管生成的数量高于B组(P＜0.05).2种治疗方法均使血浆内皮素水平显著下降(P＜0.05),但下降程度差异无统计学意义(P>0.05).结论 骨髓动员与动员后单个核细胞移植均能促进缺血后肢毛细血管的生成,尤以动员后单个核细胞移植效果显著.2种方法均可改善糖尿病大鼠的内皮功能,两者间差异无统计学意义.     

环磷酰胺、煤焦油对豫医无毛小鼠骨髓细胞微核的影响

目的探讨豫医无毛小鼠(YYHL)及昆明小鼠骨髓嗜多染红细胞自发微核率及化学诱变剂对其微核形成的影响.方法将小鼠分成6组,分别为YYHL组,昆明小鼠组,YYHL和昆明小鼠注射环磷酰胺组,及YYHL和昆明小鼠涂煤焦油组.采用骨髓嗜多染红细胞微核技术观察骨髓细胞微核率.结果①YYHL嗜多染红细胞自发微核率与正常昆明小鼠比较差异无显著性(P＞0.05).②腹腔注射环磷酰胺及煤焦油涂颈背部皮肤后,YYHL和昆明小鼠骨髓微核率均显著升高(P＜0.05),但2组比较差异无显著性(P＞0.05).结论YYHL对煤焦油及环磷酰胺致突变作用的反应与昆明小鼠一致,是一种评价化学物遗传毒性的有效动物模型.     

人脐血细胞移植对高血糖模型小鼠的治疗作用

目的 探讨尾静脉移植人脐血细胞对糖尿病模型小鼠血糖的影响.方法 选择60只昆明小白鼠,随机抽取5只作为对照组,其余55只采用腹腔注射链脲佐菌素(STZ)的方法制备糖尿病鼠模型.成模后将小鼠随机分成移植组(26只)和糖尿病组(12只).将分离的人脐血单个核细胞于对数增长期用PKH26标记后,以2×106/ml经尾静脉注射入移植组小鼠体内.于注射后第4天和第14天观察3组小鼠的血糖和胰岛素水平,并用流式细胞仪和荧光显微镜观察PKH26标记的人脐血单个核细胞在体内的分布.结果 人脐血单个核细胞植入第4天,移植组小鼠血糖和胰岛素水平与糖尿病组、对照组比较,差异均有统计学意义(P＜0.05);移植第14天移植组小鼠血糖水平及胰岛素水平与糖尿病组比较,差异均无统计学意义(P＞0.05).流式细胞检测发现移植第4天的糖尿病小鼠的胰、脾、肝、骨髓组织内均可见PKH26+细胞,而移植第14天上述各部位未发现PKH26+细胞,荧光显微镜观察上述部位的冷冻切片结果也支持这一结果.结论 将人脐血细胞移植到未用免疫抑制剂的糖尿病小鼠体内可存活,并可以在一定时间内降低糖尿病小鼠的血糖水平.     

颅内外动脉搭桥术联合BMMNCs治疗缺血性脑卒中疗效观察

目的:探究颅内-外动脉搭桥术联合骨髓单个核细胞(bone marrow mononuclear cells,BMMNCs)治疗缺血性脑血管病临床疗效.方法:选择于2016年1月-2018年1月间河南大学第一附属医院收治的缺血性脑血管病患者作为研究对象,随机抽出80例,将80例缺血性脑血管病患者随机分为对照组和观察组,每组各40例.对照组给予颅内外动脉搭桥术+血清注射,观察组给予颅内外血管搭桥手术+BMMNCs经搭桥血管注射.对比两组缺血性脑血管病患者术前及术后3个月、6个月、12个月、24个月NIHSS评分和治疗前后梗死脑组织面积.结果:术前两组缺血性脑血管病患者的NIHSS评分对比差异无统计学意义(P>0.05);术后3、6、12、24个月,两组缺血性脑血管病患者的NIHSS评分对比差异均有统计学意义(P<0.05).治疗前两组缺血性脑血管病患者梗死脑组织面积比较差异无统计学意义(P>0.05),治疗后,观察组患者梗死部位面积缩小程度明显大于对照组(P<0.05).结论:颅内-外动脉搭桥术联合BMMNCs治疗缺血性脑血管病临床疗效确切,能够明显改善患者梗死情况,减少神经功能损伤,具有较高的应用价值.     

鹿龙再生汤对再障小鼠外周血单个核细胞及脾teff细胞T-bet和GATA-3基因表达的影响

目的 探讨鹿龙再生汤对再生障碍性贫血(简称再障)模型小鼠外周血单个核细胞以及脾CD4+CD25-细胞(teff细胞)中T-bet、GATA-3基因表达的影响.方法 通过IFN-γ腹腔注射加白消安灌胃的方法建造40只再障小鼠模型,分别予同体积的生理盐水、一般浓度、5倍浓度和10倍浓度的鹿龙再生汤进行灌胃治疗,分别归为再障组、再障小剂量组、再障中剂量组和再障大剂量组(n=10);另取40只正常小鼠按上述方法进行分组处理,分别归为正常组、正常小剂量组、正常中剂量组和正常大剂量组(n=10).通过骨髓活检病理学检查观察用药后各组小鼠的骨髓造血变化,免疫磁珠分选各组小鼠脾teff细胞,利用RT-PCR技术检测各组小鼠外周血单个核细胞以及脾teff细胞T-bet、GATA-3基因mRNA的表达情况.结果 (1)与再障组相比,再障中剂量组和再障大剂量组小鼠的骨髓造血有所恢复,尤其是再障大剂量组.(2)再障大剂量组小鼠的外周血单个核细胞以及脾teff细胞T-bet基因mRNA的表达、T-bet/GATA-3的比值较再障组均明显下降(P＜0.05),而GATA-3基因mRNA的表达明显上调(P＜0.05).结论 大剂量鹿龙再生汤可改善再障小鼠骨髓造血功能,可能是通过调节再障小鼠外周血单个核细胞及脾teff细胞的T-bet、GATA-3基因的表达来实现.     

重组人骨形成蛋白成熟肽4对急性放射损伤小鼠造血系统的修复作用

目的探讨重组人骨形成蛋白成熟肽-4(rhBMP-4m)对~(60)Coγ射线照射引起的小鼠造血系统损伤的修复作用。方法 90只BALB/c小鼠随机分为正常对照组、模型组和rhBMP-4m治疗组,每组30只。模型组和rhBMP-4m治疗组小鼠接受~(60)Coγ射线照射,照射剂量7 Gy,全身照射200 s;正常对照组小鼠不接受照射。模型组小鼠每日腹腔注射生理盐水1.0 mL,rhBMP-4m治疗组小鼠每日腹腔注射rhBMP-4m 0.5 mg,连续治疗6 d。分别于照射后第1、3、5、7、9天检测小鼠外周血白细胞数、骨髓单个核细胞数、骨髓单个核细胞中CD34~+细胞比例;照射后第9天进行脾结节计数,并计算脾脏质量与体质量的比值(脾体比)。结果照射后第1天3组小鼠外周血白细胞计数比较差异均无统计学意义(P>0.05);照射后第3、5、7、9天模型组小鼠外周血白细胞计数低于正常对照组(P<0.01);照射后第3、5天rhBMP-4m治疗组小鼠外周血白细胞计数与模型组比较差异无统计学意义(P>0.05),照射后第7、9天rhBMP-4m治疗组小鼠外周血白细胞计数高于模型组(P<0.05)。模型组小鼠照射后各时间点骨髓单个核细胞计数均低于正常对照组(P<0.01)。照射后第1、3天rhBMP-4m治疗组小鼠骨髓单个核细胞计数与模型组比较差异无统计学意义(P>0.05),照射后第5、7、9天rhBMP-4m治疗组小鼠骨髓单个核细胞计数高于模型组(P<0.05)。模型组小鼠照射后各时间点的单个核细胞中CD34~+细胞百分率均低于正常对照组(P<0.01)。rhBMP-4m治疗组小鼠照射后第1、3天单个核细胞中CD34~+细胞百分率与模型组比较差异均无统计学意义(P>0.05),照射后第5、7、9天rhBMP-4m治疗组小鼠单个核细胞中CD34~+细胞的百分率高于模型组(P<0.05)。照射后第9天,模型组小鼠脾结节计数高于正常对照组,脾体比低于正常对照组(P<0.05);rhBMP-4m治疗组小鼠脾结节计数和脾体比高于模型组(P<0.01)。结论辐射可引起小鼠骨髓造血系统损伤,rhBMP-4m能够促进骨髓造血系统的重建。     

骨髓单个核细胞移植对急性心梗大鼠血流动力学的影响

目的:评价局部移植骨髓单个核细胞对急性心梗大鼠缺血心肌血管生成作用及血流动力学的影响.方法:将26只Lweis大鼠随机分为移植组(n=14)及对照组(n=12),结扎大鼠左冠状动脉,建立急性心肌梗死模型.将新分离的骨髓单个核细胞注射到大鼠的缺血心肌中(对照组大鼠注射等量无菌PBS),术后4周观察心肌血管数目,结合心脏血流动力学参数评价心功能的改善情况.结果:4周后移植组与对照组相比左心室舒张末期压力明显降低(P＜0.01),压力变化率最大值(dp/dt)明显升高(P＜0.01,P＜0.05).移植组心肌梗死区有明显血管增生,移植组和对照组心肌血管密度分别为(23.1±1.5)和(10.5±1.8)个/HPF,差异有显著性意义(P＜0.01).结论:局部移植骨髓单个核细胞能明显促进缺血心肌新生血管生成,增加缺血区灌注,并可在一定程度上改善其血流动力学指标.     

自体骨髓单个核细胞移植治疗陈旧性心肌梗死并心力衰竭的研究

目的:观察自体骨髓单个核细胞移植治疗陈旧性心肌梗死并心力衰竭的有效性、安全性。方法:18例陈旧性心肌梗死合并心力衰竭的患者每例取自体骨髓分离骨髓单个核细胞经冠脉途径或心外膜下注射途径进行细胞移植,选同期住院的符合入选标准梗死部位及罪犯血管与移植组相匹配的常规治疗的患者18例为对照组,随访3～6月。结果:移植组患者术后无新的心律失常、心绞痛及心肌梗死发生;与术前比较,NYHA心功能分级改善(P=0.002);6-MWT增加(P=0.005);BNP降低(P=0.000);LVEF增加(P=0.000);WMSI降低(P=0.032),SPECT显示缺损面积缩小(P=0.001),而对照组各项指标虽然也有一定改善,但未显示统计学意义(P>0.05)。结论:自体骨髓单个核细胞移植治疗陈旧性心肌梗死并心力衰竭安全有效。     

骨髓单核细胞移植对脑梗死小鼠白质损伤及血管再生的影响

目的 探讨评价骨髓单个核细胞(bone marrow mononuclear cells,BMMNCs)移植对大脑中动脉栓塞小鼠脑白质损伤的疗效及梗死周围血管再生的影响.方法 线栓法将成年雄性小鼠制成大脑中动脉栓塞模型,模型成功后按随机表法随机分成假手术组,MCAO组,PBS组及BMMNCs移植组(BMMNCs组).梯度密度离心法提取分离BMMNCs,模型成功后24 h经尾静脉移植入0.5 ml含1×106个BMMNCs细胞悬液,PBS组给予等体积的PBS溶液,神经功能学评分(mNSS)评价小鼠神经功能,luxol fast blue(LFB)染色观察梗死周围脑白质损伤情况,免疫荧光染色观察梗死周围血管再生情况.结果 除假手术组外,其他各组均有不同程度的神经功能缺损.梗死后56 d,BMMNCs组LFB着色减少百分比[(24.06±5.48)％]明显低于MCAO组[(62.22±5.38)％]及PBS组[(61.22±5.48)％],BMMNCs组血管密度[(249.8±11.8)个/mm2]明显高于MCAO组[(119.1 ±11.2)个/mm2]及PBS组[(114.3±12.1)个/mm2],差异有统计学意义(P＜0.05),MCAO组与PBS组相比,LFB着色减少百分比和血管密度差异无统计学意义(P＞0.05).结论 BMMNCs移植可促进血管新生,增加梗死周围区血管密度,减轻脑白质损伤程度,显著改善脑梗死小鼠神经功能.     

再生障碍性贫血小鼠T-bet表达及骨髓巨噬细胞对其表达的影响

目的 探讨再生障碍性贫血(简称再障)小鼠外周血单个核细胞Th1细胞转录因子T-bet的表达及骨髓巨噬细胞对正常小鼠外周血单个核细胞T-bet的影响. 方法 ①免疫介导(γ射线照射和淋巴细胞输入)的方法 建立再障小鼠模型.②RT-PCR方法 检测再障小鼠外周血单个核细胞上T-bet mRNA的表达.③正常小鼠外周血单个核细胞分别经再障小鼠骨髓基质细胞培养上清和小鼠骨髓型正常巨噬细胞株Ana-1培养上清刺激后,用RT-PCR方法 检测T-bet mRNA的表达. 结果 ①再障小鼠外周血单个核细胞T-bet mRNA的表达(1.49±0.18)较正常对照组(1.03±0.06)增高(P<0.05);②正常小鼠外周血单个核细胞分别经再障小鼠骨髓基质细胞培养上清和Ana-1细胞株培养上清刺激后,T-bet mRNA的表达分别为1.29±0.03和1.54±0.03,均较正常对照组(1.03±0.06)增高(均P<0.05). 结论 结果 提示巨噬细胞可能通过增加T-bet的表达参与再生障碍性贫血的免疫发病机制.     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.