干细胞的研究进展

在美国《Ｓｃｉｅｎｃｅ》杂志评选出的１９９９年度１０大科学进展中，干细胞（ｓｔｅｍ ｃｅｌｌ， ＳＣ）的研究工作格外令人瞩目。这不仅因为在过去短短的一年里，科学家们在干细胞的功能研究方面发表了１０余篇具有里程碑意义的论文，更重要的是，这些突破性进展使得生物学家和医学家们更进一步地认识到：ＳＣ作为一类既有自我更新能力、又有多分化潜能的细胞，具有非常重要的理论研究意义和临床应用价值［１］。这些成果一方面揭示了许多有关细胞生长和发育的基础理论难题；另一方面，可望将其用于创伤修复、神经再生和抗衰老等临床医…     

干细胞移植治疗肝脏疾病的研究进展

背景：干细胞移植治疗肝病是近年来众多学者研究的热点,干细胞移植的基本理论和临床应用研究都取得了很大的进展。 目的：对干细胞移植的理论依据、干细胞来源、移植方式、实验与临床研究、存在问题及前景进行简要综述。 方法：应用计算机检索中国学术期刊全文数据库(CNKI)和Pubmed数据库中2001-01/2011-11关于干细胞移植治疗肝病的文章,检索主题词“干细胞,移植,肝脏疾病,肝损伤”或“stem cell,transplantation,hepatic disease, hepatic injury”。初检索到192篇文献,据纳入标准保留31篇进行分析、综述。 结果与结论：干细胞来源充足,容易获取,可以体外增殖培养,干细胞移植操作简单,安全性高,尤其自体干细胞移植可完全避免移植排斥反应。但自体干细胞移植肝脏疾病的安全性和有效性尚无公识,需更长期的观察。     

胚胎神经干细胞移植治疗阿尔茨海默病

BACKGROUND:More recently, stem cell therapy has become an issue of concern. Exogenous neural stem cell transplantation brings new hope for the treatment of nervous system injury by self-replication and differentiation to complement and replace damaged or dead nerve cells. OBJECTIVE:To explore the therapeutic efficacy of neural stem cell transplantation on Alzheimer’s disease. METHODS:Thirty APP/PS1 mice with Alzheimer’s disease were randomly assigned into model group, cell solution transplantation group or cell transplantation group (n=10 per group). Another 10 C57BL/6 mice were selected as controls. Embryos of C57BL/6 mice at 18 embryonic days were taken to make neural stem cell suspension followed by transfection using lentiviral vectors carrying GFP gene at different multiplicities of infection (1, 5, 10, 15, 20). Afterwards, GFP-transfected neural stem cells were implanted into the hippocampus of Alzheimer’s disease mice in the cell transplantation group, while the same volume of complete medium was injected into the hippocampus of mice in the cell solution transplantation group. Morris water maze test was performed at 2 weeks after cell transplantation, and brain tissues of mice was taken and detected histologically at 4 weeks after cell transplantation. RESULTS AND CONCLUSION:Compared with the control group, the escape latency was significantly higher, and the number of crossings over the target quadrant was lower in the other three groups (P < 0.05). Compared with the cell solution transplantation and model groups, in contrast, the escape latency was significantly lower, and the number of crossings over the target quadrant was significantly higher in the cell transplantation group (P < 0.05). Four weeks after transplantation, more intact neurons were found in the cell transplantation group as compared with the model group. These findings indicate that neural stem cell transplantation can improve behavior and morphology performance of mice with Alzheimer’s disease.     

干细胞移植治疗系统性硬化的研究进展

系统性硬化(systemic sclerosis,SSc)的治疗一直是临床上的难题,现有的治疗手段难以满足患者需求。造血干细胞移植(hematopoietic stem cell transplantation,HSCT)在临床上的发展已相对成熟,近年来其在SSc上的临床试验取得了突破进展,但移植相关并发症问题一直有待...     

神经干细胞移植治疗老年痴呆

BACKGROUND:Neural stem cell transplantation has been used to treat a series of brain injury diseases, such as cerebral palsy, but its effect on Alzheimer’s disease is rarely reported. OBJECTIVE:To observe the effect of neural stem cell transplantation on the behavior and immune regulating system of Alzheimer’s disease rats.  METHODS:Thirty-five Sprague-Dawley rats were enrolled to make a postcerebral incision and given hippocampal injection of amanita phalloides acid to establish rat models of Alzheimer’s disease. Another 10 rats were only given hippocampal injection of normal saline after preparation of postcerebral skin incision as sham operation group. Then 32 successful rat models were randomly divided into two groups (n=16 per group): rats in experimental group were administrated hippocamal injection of 5×109/L allogeneic neural stem cell suspension; those in model group were given no injection. Five-day Morris water maze test was conducted at 4 weeks after transplantation. At 1 week after Morris water maze test, levels of interleukin-1 and interleukin-10 in the cerebral homogenate were detected, as well as pathological changes of brain tissues were observed in the three groups. RESULTS AND CONCLUSION:Compared with the model group, the abilities of cognition and memory were significantly higher in the sham operation group (P < 0.01), and the abilities of spatial learning and memory were significantly higher in the experimental group (P < 0.05, P < 0.01). Levels of interleukin-1 and interleukin-10 in the model group were significantly higher than those in the sham operation group (P < 0.01) but significantly lower than those in the experimental group (P < 0.01). Besides, the number of neurons in the model group was obviously less than that in the experimental and sham operation group. These results indicate that neural stem cell transplantation supplements and protects neurons against Alzheimer's disease in rats, thereby significantly improving the learning and memory ability.     

同基因与异基因胚胎肝干细胞移植治疗小鼠肝硬化

背景：胚胎肝干细胞移植免疫相关研究较少,同基因与异基因胚胎肝干细胞移植对小鼠肝硬化的治疗作用,目前尚不清楚。 目的：观察同种同基因与同种异基因胚胎肝干细胞移植对小鼠肝硬化的治疗作用,以及治疗过程中免疫排斥反应发生情况。 方法：采用Ⅳ型胶原酶消化法分离纯化BALB/c与C57BL/6胚胎肝干细胞。104只健康BALB/c小鼠随机分为4 组：正常对照组不予任何处理;肝硬化组、同种同基因移植组、同种异基因移植组腹腔注射四氯化碳石蜡油溶液复制肝硬化模型,16周后分别经其尾静脉注射生理盐水,等量同种同基因胚胎肝干细胞和同种异基因胚胎肝干细胞。在移植4周后比较各组受体小鼠存活情况、肝功能恢复情况、肝纤维化程度、免疫细胞(CD4⁺T、CD8⁺T、NK、NKT)数目及比值、肝脏病理学变化。 结果与结论：同种同基因移植组和同种异基因移植组生存率均为100%,与肝硬化组小鼠存活率67%相比差异有显著性意义(P < 0.05);各组肝功能和肝纤维化指标差异无显著性意义(P > 0.05)。各组免疫学指标比较差异无显著性意义(P > 0.05)。肝脏组织病理学显示肝组织修复：同种异基因移植组>同种同基因移植组>肝硬化组。因此,经尾静脉移植胚胎肝干细胞能提高肝硬化小鼠的生存率、减轻肝细胞坏死程度;同种同基因与同种异基因胚胎肝干细胞移植未发现免疫排斥,对小鼠肝硬化有一定的治疗作用。     

自体骨髓干细胞移植治疗肝硬化的疗效观察

治疗终末期肝病,原位肝移植是最理想的手段,然而供体短缺、手术损伤大、术后的免疫排斥反应以及费用高昂等问题限制了肝移植技术的发展和临床应用。自体骨髓干细胞移植操作简便、有效、侵入性小且并发症少,具有重要的临床意义。我科2009年9月至2011年1月共收治严重肝硬化失代偿期患者8例,经自体骨髓干细胞移植,治疗效果良好     

干细胞移植治疗心肌坏死的基础与临床研究

干细胞治疗心肌坏死是一种新的很有前景的治疗手段,但其机制尚不十分清楚。我国目前临床研究发现干细胞移植可以明显改善急性心肌梗死及梗死后心衰心脏功能,但由于关于干细胞移植的安全性还没有定论,因此还需慎重对待。     

糖原合成酶激酶3β过表达心肌干细胞移植治疗心肌梗死

BACKGROUND:The mechanism and effect of glycogen synthase kinase 3β (GSK-3β) in the differentiation of cardiac stem cells into cardiomyocytes are still unclear, although GSK-3β is closely related to the life activities of cells. OBJECTIVE:To investigate the changes of GSK-3β expression in the treatment of myocardial infarction in rats undergoing cardiac stem cell transplantation. METHODS:The isolation and culture of cardiac stem cells were performed in 10 neonatal rats. Lentivirus overexpressing GSK-3β or LacZ (control) was constructed and transferred into cardiac stem cells. Animal model of myocardial infarction was made in 30 Sprague-Dawley rats. Six weeks after model preparation, rat models were assigned into GSK-3β, LacZ or PBS group. GSK-3β or LacZ overexpressing cardiac stem cell solution or PBS in equal volume was injected into the rat myocardium, respectively. Four weeks after transplantation, the cardiac function and myocardial collagen production in rats were detected and compared. RESULTS AND CONCLUSION:Compared with the other two groups, the left ventricular ejection fraction was significantly higher, and the left ventricular end diastolic diameter was significantly lower in the GSK-3β group (P < 0.05). Hydroxyproline content, type I collagen mRNA, and type III collagen mRNA expression were significantly lower in the GSK-3β group than the other two groups (P < 0.05). Findings from Masson staining showed that the content of blue-stained collagen was significantly lower in the GSK-3β group than the LacZ group. Moreover, lowest myocardial infarction size was found in the GSK-3β group (P < 0.05). All these experimental findings show that GSK-3 overexpression plays a positive role in promoting the therapeutic effect of cardiac stem cell transplantation.     

胚胎干细胞和异种器官移植——21世纪移植免疫学面临的挑战

1998年美国Ｔｈｏｍｓｏｎ和Ｇｅａｒｈａｒｔ两个实验室分别报告人体胚胎干细胞 (ＥＳ细胞 )和胚胎生殖细胞(ＥＧ细胞 )建系成功 ,由此激起人体ＥＳ细胞定向分化的研究。美国《时代》周刊将此列为 2 0世纪末世界十大科技成就之首 ,并认为ＥＳ细胞和人类基因组将同时成为新世纪最具发展和应用前景的领域。1　ＥＳ细胞的定向分化与移植应用核转移技术 ,人体体细胞核可被植入去核卵母细胞中 ,通过胚泡阶段的发育 ,从中获取内细胞团 (ＩＣＭ ) ,建成ＥＳ细胞系。此体细胞核已被ｒｅ ｐｒｏｇｒａｍｍｅｄ ,具有多潜能性 ,能定向分化成各种细胞…     

骨髓间充质干细胞移植心肌梗死大鼠的心功能

背景：前期研究发现移植的骨髓间充质干细胞能在受损心肌组织内存活和分化。 目的：进一步观察局部注射移植同种异体骨髓间充质干细胞对心肌梗死模型大鼠心功能的影响。 方法：体外培养的同种异体骨髓间充质干细胞达到一定数量(10⁶)后用BrdU标记。24只SD大鼠随机分为3组：正常对照组未行冠状动脉前降支结扎,取骨髓间充质干细胞约1 mL直接注射到冠状动脉前降支心肌的周围;假移植组：在冠状动脉前降支结扎后7 d,取等量DMEM培养液直接注射到梗死心肌的周围;骨髓间充质干细胞移植组：冠状动脉前降支结扎后7 d,取等量骨髓间充质干细胞直接注射到梗死心肌的周围;分别于移植前、移植后5周测定大鼠心功能。 结果与结论：①移植后5周假移植组最大左室收缩末压、左室内压最大(最小)变化速率均低于移植前(P < 0.01),而左室舒张末压高于移植前(P < 0.01);移植后5周骨髓间充质干细胞移植组最大左室收缩末压、左室内压最大(最小)变化速率高于移植前(P < 0.01),而左室舒张末压低于移植前(P < 0.01)。②移植后5周,骨髓间充质干细胞移植组大鼠的最大左室收缩末压、左室内压最大(最小)变化速率均高于假移植组(P < 0.01),而左室舒张末压明显低于假移植组(P < 0.01)。结果可见骨髓间充质干细胞移植可明显改善心肌梗死模型大鼠心功能。     

人工脑膜复合大鼠骨髓间充质干细胞修复心肌梗死

背景：干细胞移植治疗心肌梗死拥有广泛的应用前景,寻求理想的细胞类型和有效的移植方式是提高干细胞治疗效果的关键因素。 目的：探讨人工脑膜复合骨髓间充质干细胞修复心肌梗死的安全性及作用。 方法：采用全骨髓贴壁筛选法分离培养骨髓间充质干细胞,取培养良好的第3代骨髓间充质干细胞经DAPI标记后接种于人工脑膜制备细胞人工脑膜复合物。构建SD大鼠心肌梗死模型,60只大鼠随机数字表法均分为假手术组、心肌梗死组、人工脑膜组、细胞脑膜复合物组。移植4 周后检测心功能参数,Western blot检测心肌组织缝隙连接蛋白43的表达,计算心肌梗死后生存率。 结果与结论：构建心肌梗死模型并移植后4周,细胞脑膜复合物组心脏组织冰冻切片于荧光显微镜下可观察到心肌内少量核蓝染的细胞,表明骨髓间充质干细胞得以存活;细胞脑膜复合物组与心肌梗死组和人工脑膜组相比,左心室功能明显改善,Cx43蛋白的表达上调,生存率增加(P < 0.05)。说明人工脑膜复合骨髓间充质干细胞移植可提高心肌梗死大鼠心脏功能及生存率。     

同种异体骨髓间充质干细胞移植在心肌梗死后心室重建中的作用

BACKGROUND:Myocardial infarction leads to ischemic changes in the myocardium, triggering the emergence of ventricular remodeling, which is an important cause of death. Myocardial infarction is a common disease in the middle-aged and elderly population, but autologous bone marrow mesenchymal stem cells from these patients exhibit a weak ability of proliferation and differentiation. Therefore, a positive attempt of allogeneic stem cell transplantation is required in order to obtain better therapeutic outcomes. OBJECTIVE:To explore the effect of allogeneic bone marrow mesenchymal stem cells on ventricular remodeling after myocardial infarction.   METHODS:Bone marrow mesenchymal stem cells from 10 neonatal rats and 10 adult rats were isolated, cultured and identified. Another 40 rats were randomly assigned into four groups (n=10/group): model group, neonatal rat cell transplantation group, adult rat cell transplantation group, or sham group. Animal models of myocardial infarction were made in rats in the all groups except for the sham group in which the rats were given sham operation. Rats in the two cell transplantation groups were given the corresponding cell transplantation. Four weeks postoperatively, heart function of rats was detected in each group, and cardiac tissues were taken to detect changes in collagen formation and blood vessel density in the infarct area. RESULTS AND CONCLUSION:Four weeks after surgery, rats in the model group showed significant changes in cardiac function indexes as compared with the other groups (P < 0.05), while compared with the model group, these cardiac function indexes improved in both two cell transplantation groups, but there was no significant difference between the two cell transplantation groups (P > 0.05). Meanwhile, compared with the model group, significantly decreased collagen formation and increased blood vessel density were found in both two cell transplantation groups (P < 0.05). Additionally, the vascular density of the infarct area was highest in the sham group (P < 0.05). Experimental results show that both neonatal and adult rat bone marrow mesenchymal stem cell transplantation can improve cardiac function of rats, reduce the formation of collagen in the infarct area and delay ventricular remodeling after myocardial infarction.     

骨髓间充质干细胞移植治疗大鼠扩张型心肌病

1泸州医学院附属医院急诊科,四川省泸州市 646000;2哈尔滨医科大学附属第二医院心内科,黑龙江省哈尔滨市  150000 背景：扩张型心肌病所致的心肌纤维化是心力衰竭的病理基础,目前药物治疗、介入治疗和外科手术均不能替代坏死心肌和彻底改善心脏功能。 目的：观察异体骨髓间充质干细胞移植对大鼠扩张型心肌病心脏功能的作用和心肌纤维化的影响。 方法：40只Wistar大鼠随机数字表法分为细胞移植组(n=15)、对照组(n=15)和空白组(n=10),前2组建立大鼠扩张型心肌病模型。造模成功4周后细胞移植组注射骨髓间充质干细胞悬液150 μL(含3×106个细胞),对照组和空白组注射等量培养液。 结果与结论：与空白组相比,细胞移植组和对照组移植前左室收缩末期内径增加,射血分数和缩短分数明显下降(P < 0.01);移植后4周,细胞移植组超声心动图检查和移植前相比,左室收缩末期内径下降、射血分数和缩短分数明显升高(P < 0.01)。细胞移植组心脏胶原的表达低于对照组(P < 0.05)。与对照组相比,其他2组基质金属蛋白酶2及基质金属蛋白酶9表达明显下降(P < 0.05)。提示骨髓间充质干细胞移植后可改善心肌纤维化及扩张型心肌病鼠的心脏功能。      

过氧化物酶体增殖物激活受体γ促进外源性骨髓间充质干细胞表达Cx43的作用及机制

BACKGROUND:Piglitazone, a peroxisome proliferator-activated receptor γ (PPAR-γ) agonist, has been demonstrated to promote survival and cardiac differentiation of exogenous bone marrow mesenchymal stem cells to improve cardiac function. In this study, we attempted to investigate whether pioglitazone could induce cardiac differentiation of endogenous bone marrow mesenchymal stem cells and improve cardiac function, and meanwhile, probed into the relevant mechanisms. OBJECTIVE:To compare the therapeutic efficacy of pioglitazone combined with bone marrow mesenchymal stem cell transplantation, pioglitazone alone and phosphate buffer solution (PBS) and to investigate the relevant mechanisms. METHODS:Thirty Sprague-Dawley rats with myocardial infarction induced by ligation of the left anterior descending coronary artery were randomized into combined group (combination of bone marrow mesenchymal stem cells and pioglitazone), pioglitazone group and PBS group. Two weeks later, PKH26-labeled bone marrow mesenchymal stem cells in PBS or PBS alone were injected into the local infarct zone in the combined group and the other two groups, respectively. Pioglitazone (3 mg/kg/d) was given by the oral gavage in the combined and pioglitazone groups for continuous 2 weeks after cells transplantation. At 2 weeks after treatment, cardiac functions were evaluated. In addition, expressions of PPAR-γ, connexin 43 and relative factors in transforming growth factor-β1/SMAD signaling pathway were examined in different areas of the left ventricle from each harvested heart. RESULTS AND CONCLUSION:There were no differences in the baseline parameters of cardiac function between the two groups. Two weeks after treatment, left ventricular end-diastolic diameter, left ventricular end-systolic diameter and left ventricular ejection fraction were significantly improved in the combined group compared with the other two groups; the expression of PPAR-γ was significantly increased in different zones of the left ventricle in the combined and pioglitazone groups. In the combined group, there was a significantly higher expression of connexin 43, and the levels of transforming growth factor-β1, SMAD2 and SMAD3 were obviously attenuated in the infarct and marginal zones. However, no differences were found in the above determinants between the pioglitazone and PBS groups. To conclude, pioglitazone cannot induce the differentiation and proliferation of endogenous bone marrow mesenchymal stem cells, but pioglitazone combined with exogenous bone marrow mesenchymal stem cells can improve cardiac function post myocardial infarction. In this process, PPAR-γ might promote the connexin 43 expression in exogenous bone marrow mesenchymal stem cells via the blockade of transforming growth factor-β1/SMAD signaling pathway.     

骨髓间充质干细胞在急性心肌梗死后心室重构中的应用☆

背景：动物实验和初期的临床研究表明,干细胞移植可以取代坏死心肌细胞、增加有功能心肌细胞的数量,改善心功能,从而为心肌梗死的治疗开辟了一条崭新的途径。 目的：观察心肌梗死后自体骨髓间充质干细胞移植及骨髓动员对心功能的影响。 方法：经猪髂前上棘抽取骨髓30 mL,培养得到骨髓间充质干细胞。15只猪分为3组,模型组仅建立心肌梗死模型,干细胞移植组在造模3 h后经冠状动脉注射骨髓间充质干细胞,干细胞动员组在造模3 h后连续5 d注射粒细胞集落刺激因子    150 μg/(kg•d)。 结果与结论：心肌梗死后8周,干细胞动员组、干细胞移植组左心室收缩、舒张末期内径都明显减小,射血分数较心肌梗死前提高,但差异无显著性意义(P > 0.05);干细胞动员组及干细胞移植组血清血管内皮生长因子水平较心肌梗死前有上升趋势(P < 0.05);干细胞动员组和干细胞移植组梗死交界区的毛细血管密度均大于模型组(P < 0.05)。提示心肌梗死后行自体骨髓间充质干细胞移植及骨髓动员均能明显改善心功能,但具体效果仍需进一步大样本实验研究。 关键词：缺血性心脏病;心力衰竭;骨髓间充质干细胞;血管再生;粒细胞集落刺激因子 doi:10.3969/j.issn.1673-8225.2012.10.009     

骨髓间质干细胞减轻收缩期高血压的心肌损伤

BACKGROUND:Mesenchymal stem cells can be used to treat a variety of injuries, but little is known about their effect on systolic hypertension. OBJECTIVE:To study the therapeutic effect of bone marrow mesenchymal stem cells on systolic hypertension rats. METHODS:Twenty-four rats were randomized equally into control, model and cell transplantation groups. A rat model of systolic hypertension was made by injection of vitamin K and warfarin sodium in the model and cell transplantation groups. Rats in the cell transplantation group were given injection of bone marrow mesenchymal stem cells into the left ventricle, while those in the other two groups given normal saline. RESULTS AND CONCLUSION:Compared with the model group, bone marrow mesenchymal stem cells could reduce left ventricular mass index, increase cardiac tissue type I collagen volume fraction, collagen volume fraction and perivascular collagen volume fraction, but the ratio of end-diastolic volume/body weight did not change significantly. These findings indicate that bone marrow mesenchymal stem cell transplantation can reduce myocardial hypertrophy and cardiac fibrosis in systolic hypertension rats.     

血管内皮生长因子基因修饰骨髓间充质干细胞移植心肌梗死大鼠： 高压氧干预促进治疗性血管生成

背景：骨髓间充质干细胞移植入缺血心肌后梗死区周边心肌的缺血、缺氧、炎性反应、细胞凋亡等病理变化严重影响到移植骨髓间充质干细胞的存活,而高压氧能显著改善其缺氧状态。 目的：对心肌梗死模型大鼠缺血心肌局部移植血管内皮生长因子修饰的骨髓间充质干细胞,在移植后对大鼠进行高压氧干预,探讨其对骨髓间充质干细胞移植后所获得的治疗性血管生成效应的影响。 方法：将真核表达载体pcDNA3.1(-)/人血管内皮生长因子165转染大鼠骨髓间充质干细胞,再移植至心肌梗死模型大鼠的缺血区组织,移植前细胞行CM-DiI标记。移植后2周每日对大鼠行高压氧治疗干预。移植1个月后行心脏B超测量其左室射血分数,组织化学苏木精-伊红染色和Ⅷ因子染色并评价新生血管密度。 结果与结论：CM-DiI能有效标记骨髓间充质干细胞,标记率近100%。细胞移植1个月后高压氧干预的大鼠射血分数、再生血管密度较均明显增高(P < 0.05)。证实,高压氧干预能显著促进移植血管内皮生长因子基因修饰的骨髓间充质干细胞的心肌梗死大鼠所获得的治疗性血管生成作用,对心脏功能有明显改善作用。     

PTEN基因沉默骨髓间充质干细胞移植治疗急性心肌梗死

BACKGROUND:Bone marrow mesenchymal stem cell transplantation for myocardial infarction becomes popularized in recent years, but transplanted cells cannot survive and proliferate under early inflammatory reaction or local ischemia/hypoxia microenvironment, eventually hampering the therapeutic outcomes. OBJECTIVE:To investigate the therapeutic effect of PTEN-silenced bone marrow mesenchymal stem cells on acute myocardial infarction. METHODS:(1) Bone marrow mesenchymal stem cells from Sprague-Dawley rats were randomly assigned to receive no treatment, NCsiRNA transfection using Lipofectamin2000 or PTEN siRNA transfection using Lipofectamin2000. Cell growth curves were described using MTT method to detect cell cycle using flow cytometry. (2) Thirty Sprague-Dawley rats were selected to prepare myocardial infarction models that were randomized into three groups (n=10 per group): blank control, negative control and RNAi group. Six hours after modeling, bone marrow mesenchymal stem cells transfected with nothing, NCsiRNA and PTEN siRNA were respectively injected into the infarcted center of the left ventricular anterior wall in these three rat groups. After 4 weeks, all rats were subjected to cardiac function detection using echocardiography, and the survival and proliferation of bone marrow mesenchymal stem cells in the rats were observed by fluorescence microscopy. RESULTS AND CONCLUSION:Compared with the other two groups, a significant increase in the absorbance values at different culture time, the proportion of cells in S+G2 phase, and the number of bone marrow mesenchymal stem cells in the myocardial tissue was found in the RNAi group (all P < 0.05). Additionally, the left ventricular ejection fraction and left ventricular shortening fraction were significantly reduced in the RNAi group than the blank control and negative control groups at 4 weeks after cell transplantation (P < 0.05). Both in vivo and in vitro experimental findings showed that PTEN silencing could effectively improve cell survival and proliferation in the infarcted myocardium. Moreover, in the in vivo experiment, an overt improvement in rat’s cardiac function was achieved.      

自体骨髓间充质干细胞移植梗死心肌猪的心功能变化

背景：干细胞移植可改善缺血性心肌血供并改善心功能。 目的：进一步验证自体骨髓间充质干细胞在心肌梗死后的应用及效果评价。 方法：15只健康太湖梅山猪通过冠脉栓塞建立心肌梗死模型。随机分为3组,每组5只,其中2组分别在心肌梗死后3 h,2周行自体骨髓干细胞移植,模型组不移植细胞。行心脏超声观察心脏功能各指标的改变;并在不同时间点检测血清血管内皮生长因子值;在实验终点取大体标本并通过免疫组织化学检测移植细胞在心肌内定植及分化情况,检测心肌血管密度。 结果与结论：心肌梗死3 h组与模型组比较,射血分数、左室舒张期内径、左室收缩期内径各项心功能指标及心肌血管密度、不同时间点血清血管内皮生长因子水平差异无显著性意义。心肌梗死后2周移植组与模型组相比,心功能指标均有改善,心肌血管密度大于模型组,血清血管内皮生长因子水平明显高于较移植前(P < 0.05)。提示不同时间点心肌微环境对于骨髓来源的间充质干细胞分化及定植的影响,心肌梗死后急性期内局部微环境不利于移植细胞的存活,在瘢痕修复早期行骨髓干细胞移植对骨髓干细胞的分化和定植以及对心功能的改善有较好的效果。