干细胞的研究进展

在美国《Ｓｃｉｅｎｃｅ》杂志评选出的１９９９年度１０大科学进展中，干细胞（ｓｔｅｍ ｃｅｌｌ， ＳＣ）的研究工作格外令人瞩目。这不仅因为在过去短短的一年里，科学家们在干细胞的功能研究方面发表了１０余篇具有里程碑意义的论文，更重要的是，这些突破性进展使得生物学家和医学家们更进一步地认识到：ＳＣ作为一类既有自我更新能力、又有多分化潜能的细胞，具有非常重要的理论研究意义和临床应用价值［１］。这些成果一方面揭示了许多有关细胞生长和发育的基础理论难题；另一方面，可望将其用于创伤修复、神经再生和抗衰老等临床医…     

干细胞移植治疗肝脏疾病的研究进展

背景：干细胞移植治疗肝病是近年来众多学者研究的热点,干细胞移植的基本理论和临床应用研究都取得了很大的进展。 目的：对干细胞移植的理论依据、干细胞来源、移植方式、实验与临床研究、存在问题及前景进行简要综述。 方法：应用计算机检索中国学术期刊全文数据库(CNKI)和Pubmed数据库中2001-01/2011-11关于干细胞移植治疗肝病的文章,检索主题词“干细胞,移植,肝脏疾病,肝损伤”或“stem cell,transplantation,hepatic disease, hepatic injury”。初检索到192篇文献,据纳入标准保留31篇进行分析、综述。 结果与结论：干细胞来源充足,容易获取,可以体外增殖培养,干细胞移植操作简单,安全性高,尤其自体干细胞移植可完全避免移植排斥反应。但自体干细胞移植肝脏疾病的安全性和有效性尚无公识,需更长期的观察。     

胚胎神经干细胞移植治疗阿尔茨海默病

BACKGROUND:More recently, stem cell therapy has become an issue of concern. Exogenous neural stem cell transplantation brings new hope for the treatment of nervous system injury by self-replication and differentiation to complement and replace damaged or dead nerve cells. OBJECTIVE:To explore the therapeutic efficacy of neural stem cell transplantation on Alzheimer’s disease. METHODS:Thirty APP/PS1 mice with Alzheimer’s disease were randomly assigned into model group, cell solution transplantation group or cell transplantation group (n=10 per group). Another 10 C57BL/6 mice were selected as controls. Embryos of C57BL/6 mice at 18 embryonic days were taken to make neural stem cell suspension followed by transfection using lentiviral vectors carrying GFP gene at different multiplicities of infection (1, 5, 10, 15, 20). Afterwards, GFP-transfected neural stem cells were implanted into the hippocampus of Alzheimer’s disease mice in the cell transplantation group, while the same volume of complete medium was injected into the hippocampus of mice in the cell solution transplantation group. Morris water maze test was performed at 2 weeks after cell transplantation, and brain tissues of mice was taken and detected histologically at 4 weeks after cell transplantation. RESULTS AND CONCLUSION:Compared with the control group, the escape latency was significantly higher, and the number of crossings over the target quadrant was lower in the other three groups (P < 0.05). Compared with the cell solution transplantation and model groups, in contrast, the escape latency was significantly lower, and the number of crossings over the target quadrant was significantly higher in the cell transplantation group (P < 0.05). Four weeks after transplantation, more intact neurons were found in the cell transplantation group as compared with the model group. These findings indicate that neural stem cell transplantation can improve behavior and morphology performance of mice with Alzheimer’s disease.     

干细胞移植治疗系统性硬化的研究进展

系统性硬化(systemic sclerosis,SSc)的治疗一直是临床上的难题,现有的治疗手段难以满足患者需求。造血干细胞移植(hematopoietic stem cell transplantation,HSCT)在临床上的发展已相对成熟,近年来其在SSc上的临床试验取得了突破进展,但移植相关并发症问题一直有待...     

神经干细胞移植治疗老年痴呆

BACKGROUND:Neural stem cell transplantation has been used to treat a series of brain injury diseases, such as cerebral palsy, but its effect on Alzheimer’s disease is rarely reported. OBJECTIVE:To observe the effect of neural stem cell transplantation on the behavior and immune regulating system of Alzheimer’s disease rats.  METHODS:Thirty-five Sprague-Dawley rats were enrolled to make a postcerebral incision and given hippocampal injection of amanita phalloides acid to establish rat models of Alzheimer’s disease. Another 10 rats were only given hippocampal injection of normal saline after preparation of postcerebral skin incision as sham operation group. Then 32 successful rat models were randomly divided into two groups (n=16 per group): rats in experimental group were administrated hippocamal injection of 5×109/L allogeneic neural stem cell suspension; those in model group were given no injection. Five-day Morris water maze test was conducted at 4 weeks after transplantation. At 1 week after Morris water maze test, levels of interleukin-1 and interleukin-10 in the cerebral homogenate were detected, as well as pathological changes of brain tissues were observed in the three groups. RESULTS AND CONCLUSION:Compared with the model group, the abilities of cognition and memory were significantly higher in the sham operation group (P < 0.01), and the abilities of spatial learning and memory were significantly higher in the experimental group (P < 0.05, P < 0.01). Levels of interleukin-1 and interleukin-10 in the model group were significantly higher than those in the sham operation group (P < 0.01) but significantly lower than those in the experimental group (P < 0.01). Besides, the number of neurons in the model group was obviously less than that in the experimental and sham operation group. These results indicate that neural stem cell transplantation supplements and protects neurons against Alzheimer's disease in rats, thereby significantly improving the learning and memory ability.     

同基因与异基因胚胎肝干细胞移植治疗小鼠肝硬化

背景：胚胎肝干细胞移植免疫相关研究较少,同基因与异基因胚胎肝干细胞移植对小鼠肝硬化的治疗作用,目前尚不清楚。 目的：观察同种同基因与同种异基因胚胎肝干细胞移植对小鼠肝硬化的治疗作用,以及治疗过程中免疫排斥反应发生情况。 方法：采用Ⅳ型胶原酶消化法分离纯化BALB/c与C57BL/6胚胎肝干细胞。104只健康BALB/c小鼠随机分为4 组：正常对照组不予任何处理;肝硬化组、同种同基因移植组、同种异基因移植组腹腔注射四氯化碳石蜡油溶液复制肝硬化模型,16周后分别经其尾静脉注射生理盐水,等量同种同基因胚胎肝干细胞和同种异基因胚胎肝干细胞。在移植4周后比较各组受体小鼠存活情况、肝功能恢复情况、肝纤维化程度、免疫细胞(CD4⁺T、CD8⁺T、NK、NKT)数目及比值、肝脏病理学变化。 结果与结论：同种同基因移植组和同种异基因移植组生存率均为100%,与肝硬化组小鼠存活率67%相比差异有显著性意义(P < 0.05);各组肝功能和肝纤维化指标差异无显著性意义(P > 0.05)。各组免疫学指标比较差异无显著性意义(P > 0.05)。肝脏组织病理学显示肝组织修复：同种异基因移植组>同种同基因移植组>肝硬化组。因此,经尾静脉移植胚胎肝干细胞能提高肝硬化小鼠的生存率、减轻肝细胞坏死程度;同种同基因与同种异基因胚胎肝干细胞移植未发现免疫排斥,对小鼠肝硬化有一定的治疗作用。     

自体骨髓干细胞移植治疗肝硬化的疗效观察

治疗终末期肝病,原位肝移植是最理想的手段,然而供体短缺、手术损伤大、术后的免疫排斥反应以及费用高昂等问题限制了肝移植技术的发展和临床应用。自体骨髓干细胞移植操作简便、有效、侵入性小且并发症少,具有重要的临床意义。我科2009年9月至2011年1月共收治严重肝硬化失代偿期患者8例,经自体骨髓干细胞移植,治疗效果良好     

干细胞移植治疗心肌坏死的基础与临床研究

干细胞治疗心肌坏死是一种新的很有前景的治疗手段,但其机制尚不十分清楚。我国目前临床研究发现干细胞移植可以明显改善急性心肌梗死及梗死后心衰心脏功能,但由于关于干细胞移植的安全性还没有定论,因此还需慎重对待。     

静脉移植骨髓间充质干细胞对心肌病心力衰竭心功能的影响

BACKGROUND:Bone marrow mesenchymal stem cell transplantation can effectively improve decreased cardiac function caused by heart failure, but there is a lack of research about the effect in bone marrow mesenchymal stem cell transplantation on cardiac function in heart failure induced by cardiomyopathies. OBJECTIVE:To explore the effect of bone marrow mesenchymal stem cell transplantation on cardiac function in patients with cardiomyopathies accompanied by heart failure. METHODS:Totally 40 Sprague-Dawley rats were enrolled, and bone marrow mesenchymal stem cells were isolated from 10 rats, and the remaining rats were equivalently randomized into normal, model and stem cell transplantation groups. Then rats in the model and stem cell transplantation groups were given intraperitoneal injection of hydrochloric acid doxorubicin to prepare cardiomyopathy-induced heart failure models. At 7 days after modeling, the stem cell transplantation group was treated with bone marrow mesenchymal stem cells through intravenous transplantation, and the model group was treated with equal amount of DMEM medium. Four weeks later, cardiac function of each rat was detected, and the cell survival and differentiation were observed by immunofluorescence method. RESULTS AND CONCLUSION:At 4 weeks after transplantation, compared with normal and stem cell transplantation groups, the left ventricular systolic pressure and maximum rise/fall rate of left ventricular pressure were significantly decreased, but the left ventricular end diastolic pressure significantly increased in the model group (P < 0.05). And there was no significant difference between the normal group and the stem cell transplantation group (P > 0.05). High and dense fluorescence intensity was observed in the host myocardium immediately after transplantation. Subsequently, the fluorescence intensity and density decreased at 4 weeks, but the cell migration could be found, and some cells expressed cardiac troponin T. These results show that intravenous transplantation of bone marrow mesenchymal stem cells can improve cardiac function in rats with heart failure due to cardiomyopathies. Besides, the transplanted cells can survive in the host, and differentiate into cardiomyocyte-like cells.     

干细胞可塑性的研究进展

近几年来 ,特别是 1 999年在国际权威性杂志上相继发表了几篇有关成所动物组织间叶干细胞或称间叶前体细胞 (ｍｅｓｅｎｃｈｙｍａｌｓｔｅｍｃｅｌｌｓｏｒｐｒｅｃｕｒｓｏｒｃｅｌｌｓ)可塑性 (ｐｌａｓｔｉｃｉｔｙ)的研究报告 ,打破了组织再生仅来源于潜伏在该组织内的干细胞的这一传统观念。这些研究进展被列为 2 0世纪最后一年取得的十大科技成果之一。本文综述了这一新的研究领域的进展。　　一、骨髓细胞转化为其他组织细胞的研究近年的研究证明 ,骨髓内造血干细胞不仅可分化成熟为各类造血细胞和免疫细胞 ,而且骨髓和骨膜来源的间…     

心肌干细胞调控ANG Ⅱ/AT1R/TGF-β1/SMAD/CX43通路改进心电生理学稳定性和室颤阈值

BACKGROUND:Previous studies have demonstrated that the electrophysiological stability and ventricular fibrillation threshold after myocardial infarction in rats are significantly improved in the mid-term of cardiac stem cell transplantation, but relative regulatory mechanism and pathway remain unclear. OBJECTIVE:To explore the relative molecular regulatory mechanism of cardiac stem cells improving the electrophysiological stability and ventricular fibrillation threshold after myocardial infarction in rats.  METHODS:Myocardial infarction was induced in 20 Sprague-Dawley rats by ligation of the left anterior descending coronary, which were then randomized into two groups (n=10 per group) and were subjected to the injection of cardiac stem cells labeled with PKH26 in phosphate buffer solution (cardiac stem cell group) or the same amount of phosphate buffer solution (PBS) alone (PBS group) into the local infarct zone at 2 weeks after modeling, respectively. Six weeks later, relevant signaling molecules involved in the ANGII/AT1R/TGF-β1/SMAD/Cx43 pathway were all examined in myocardial tissues of the left ventricle and harvested blood samples. RESULTS AND CONCLUSION:Compared with the PBS group, expressions of connexin 43 in different zones of the left ventricle were significantly increased in the cardiac stem cell group (P < 0.01); there was a significant reduction of the angiotensin II level in plasma and different regions of the left ventricular (P < 0.05; P < 0.01). Furthermore, in the cardiac stem cell group, expressions of angiotensin II type I receptor, transforming growth factor-β1, SMAD2 and SMAD3 were significantly decreased (P < 0.01). Whereas SMAD7 was significantly elevated (P < 0.05) in different areas of the left ventricle compared with the phosphate buffer solution group. These findings suggest that the cardiac stem cell transplantation can improve the electrophysiological stability and ventricular fibrillation threshold after myocardial infarction by enhancing the expression of connexin 43 via ANGII/AT1R/TGF-beta1/SMAD/CX43 signaling pathway.     

同种异体骨髓间充质干细胞移植在心肌梗死后心室重建中的作用

BACKGROUND:Myocardial infarction leads to ischemic changes in the myocardium, triggering the emergence of ventricular remodeling, which is an important cause of death. Myocardial infarction is a common disease in the middle-aged and elderly population, but autologous bone marrow mesenchymal stem cells from these patients exhibit a weak ability of proliferation and differentiation. Therefore, a positive attempt of allogeneic stem cell transplantation is required in order to obtain better therapeutic outcomes. OBJECTIVE:To explore the effect of allogeneic bone marrow mesenchymal stem cells on ventricular remodeling after myocardial infarction.   METHODS:Bone marrow mesenchymal stem cells from 10 neonatal rats and 10 adult rats were isolated, cultured and identified. Another 40 rats were randomly assigned into four groups (n=10/group): model group, neonatal rat cell transplantation group, adult rat cell transplantation group, or sham group. Animal models of myocardial infarction were made in rats in the all groups except for the sham group in which the rats were given sham operation. Rats in the two cell transplantation groups were given the corresponding cell transplantation. Four weeks postoperatively, heart function of rats was detected in each group, and cardiac tissues were taken to detect changes in collagen formation and blood vessel density in the infarct area. RESULTS AND CONCLUSION:Four weeks after surgery, rats in the model group showed significant changes in cardiac function indexes as compared with the other groups (P < 0.05), while compared with the model group, these cardiac function indexes improved in both two cell transplantation groups, but there was no significant difference between the two cell transplantation groups (P > 0.05). Meanwhile, compared with the model group, significantly decreased collagen formation and increased blood vessel density were found in both two cell transplantation groups (P < 0.05). Additionally, the vascular density of the infarct area was highest in the sham group (P < 0.05). Experimental results show that both neonatal and adult rat bone marrow mesenchymal stem cell transplantation can improve cardiac function of rats, reduce the formation of collagen in the infarct area and delay ventricular remodeling after myocardial infarction.     

组织工程化心肌片层移植心肌梗死大鼠的心功能及电生理变化

背景：组织工程化心肌组织在组成结构上类似于心脏组织的三维电偶联网络和肌肉横纹,而且具有心肌组织样收缩功能,为病损心肌提供了修复的可能性。 目的：观察心肌细胞/胶原复合体移植后心肌梗死大鼠心室肌的心功能及电生理变化。 方法：将成年SD大鼠分为假手术组、模型组、移植组,后2组制作心肌梗死动物模型,假手术组仅开胸,不结扎冠状动脉。移植组移植心肌细胞与胶原材料复合组织,其他2组不进行移植。 结果与结论：①左室心功能：与假手术组相比,模型组左室舒张末期内径、左室收缩末期内径均显著增大(P < 0.01),左室射血分数和左室短轴缩短率显著降低(P < 0.01);移植组左室舒张末期内径、左室收缩末期内径、左室射血分数和左室短轴缩短率均未见明显增大或降低(P > 0.01)。②左室有效不应期变化：与假手术组相比,模型组梗死周边区有效不应期显著缩短(P < 0.01);移植组梗死周边区有效不应期较模型组延长,差异有显著性意义(P < 0.01)。③Cx43免疫荧光结果：假手术组、模型组和移植组大鼠缝隙连接蛋白43阳性表达依次呈现阳性,弱阳性,弱阳性。但移植组缝隙连接蛋白43阳性表达高于模型组。结果可见移植的心肌细胞/胶原复合体在组织和结构上形成电偶联网络和收缩偶联,能改善心肌梗死大鼠心室肌的收缩功能及电生理特性。     

血红素氧合酶1提高骨髓间充质干细胞在梗死后心脏的存活

背景：移植骨髓间充质干细胞(bone mesenchymal stem cells,BMSCs)修复梗死后心功能受到围移植期移植细胞大量死亡的限制。因此寻找一种保护因子对移植细胞提供保护至关重要。 目的：观察血红素氧合酶1(heme oxygenase,HO-1)对BMSCs在梗死后心脏生存的影响。 方法：体外分离扩增培养大鼠BMSCs,Adv-hHO-1、Adv-GFP分别转染,移植前DAPI标记。结扎左前降支1 h后,分别将DAPI-hHO-1-BMSCs、DAPI-GFP-BMSCs多点注射到大鼠心脏梗死区周边,对照组注射等量PBS。 结果与结论：Adv-hHO-1转染BMSCs后获稳定表达。仅hHO-1-BMSCs组稳定表达hHO-1 mRNA;hHO-1-BMSCs组血管内皮生长因子、碱性成纤维细胞生长因子、肝细胞生长因子表达均高于其他两组(P < 0.01);存活BMSCs数量在第3,7天均明显高于GFP-BMSCs组(P < 0.05);大鼠心功能各项参数明显优于其他两组(P < 0.01)。移植4周后,HO-1-BMSCs组梗死区周边毛细血管密度明显高于GFP-BMSCs组和对照组(P < 0.01),且胶原蛋白沉积减少,心室壁变厚,梗死面积较其他两组明显缩小(P < 0.01)。提示HO-1提高移植到梗死心脏BMSCs的存活,HO-1协同BMSCs抑制心室重构,改善心功能。     

骨髓间充质干细胞改进心衰大鼠心肌细胞的收缩性

背景：骨髓间充质干细胞治疗心肌梗死已被证明能明显改善心功能。 目的：观察骨髓间充质干细胞对心肌梗死后心力衰竭大鼠心肌细胞收缩舒张功能的影响。 方法：开胸结扎10只SD大鼠左前降支冠状动脉,制备心肌缺血模型,随机抽签法分为2组,实验组造模4周后在梗死心肌内注射荧光标记的5×10¹⁰ L^-1骨髓间充质干细胞,对照组单纯注射PBS。 结果与结论：与左前降支冠状动脉结扎前相比,造模4周后两组大鼠心功能明显下降(P < 0.01);经骨髓间充质干细胞治疗后实验组的心功能得到明显改善(P < 0.05)。实验组心肌细胞收缩幅度较对照组显著增加,收缩速度显著增快(P < 0.01)。且荧光显微镜发现有经骨髓间充质干细胞分化而来具有收缩功能的心肌细胞。提示经骨髓间充质干细胞治疗后的大鼠心肌细胞其收缩舒张功能显著增强,是骨髓间充质干细胞治疗心衰大鼠导致心功能改善的主要机制之一。     

外源性硫化氢预处理骨髓间充质干细胞移植治疗大鼠心肌梗死

背景：骨髓间充质干细胞移植可促进心肌修复,治疗心肌梗死,但移植后细胞存活率低等原因制约了其应用与发展。 目的：探讨硫化氢预处理对骨髓间充质干细胞移植治疗心肌梗死效果的影响。 方法：从(100±20) g SD大鼠中分离培养骨髓间充质干细胞,第4代时按实验分组处理,移植前2 h给予DAPI标记。将50只体质量(200±20) g雄性SD大鼠分为心肌梗死组和假手术组,心肌梗死组结扎冠状动脉前降支建立心肌梗死模型,假手术组只穿线不结扎,每组10只。模型建立24 h后,分组在梗死心肌周围选择4个点,分别注射生理盐水、骨髓间充质干细胞、硫化氢预处理骨髓间充质干细胞及硫化氢。细胞移植4周后用超声心动图检测心功能指标,Masson染色测定梗死交界区胶原。 结果与结论：生理盐水组大鼠心肌纤维化严重,梗死区域无心肌组织再生;外源性硫化氢处理后的骨髓间充质干细胞移植组比单用硫化氢或者骨髓间充质干细胞组心肌纤维化程度轻,胶原组织中可见较多心肌细胞再生。硫化氢预处理骨髓间充质干细胞组左室射血分数、左室短轴缩短率显著高于硫化氢组及骨髓间充质干细胞组(P < 0.05)。提示硫化氢预处理骨髓间充质干细胞可提高移植后的细胞存活率,改善梗死后心功能,其作用优于单用硫化氢或者骨髓间充质干细胞。中国组织工程研究杂志出版内容重点：干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程全文链接：     

外周血间充质干细胞移植心肌梗死兔新生血管及心功能的变化

背景：药物治疗和支架置入治疗尚不能修复心肌梗死后已坏死的心肌。 目的：观察外周血间充质干细胞移植治疗对心肌梗死兔新生血管及心功能的影响。 方法：随机抽签法将36只大白兔分为假手术组,间充质干细胞移植组和对照组,结扎兔冠状动脉左室支建立心肌梗死模型。 结果与结论：移植后4周,流式细胞仪分析显示绝大部分间充质干细胞表达CD44,极少量细胞表达CD34和CD45。间充质干细胞移植组梗死心肌组织有移植的间充质干细胞存活,超声心动仪示间充质干细胞移植组左心室射血分数及短轴缩短率明显高于对照组(P < 0.01);左心室收缩末内径和舒张末内径明显小于对照组(P < 0.01)。间充质干细胞移植组心肌纤维化程度、心肌梗死面积均明显小于对照组(P < 0.01)。免疫组织化学染色显示间充质干细胞移植组新生毛细血管密度明显高于对照组(P < 0.01)。提示外周血间充质干细胞移植增加了梗死心肌新生血管密度,改善心脏的功能。     

胎儿脐血间充质干细胞治疗大鼠急性心肌梗死

背景：胎儿脐血间充质干细胞对病变心肌有修复和再生能力,胎儿脐血间充质干细胞移植是治疗心肌梗死的一种新途径。 目的：探讨胎儿脐血间充质干细胞移植治疗大鼠心肌梗死的效果。 方法：选取32只大鼠结扎左冠状动脉前降支制作心肌梗死动物模型,随机等分为移植组和梗死组,从胎儿脐血中分离培养脐血间充质干细胞,制备脐血间充质干细胞悬液,对移植组大鼠进行脐血间充质干细胞移植。 结果与结论：胎儿脐血间充质干细胞在体外可以被成功分离培养;与梗死组相比,移植组大鼠心肌梗死边缘区的微血管密度、左室收缩末压、左室内压最大上升和下降速率显著增加(P < 0.05),左室舒张末压显著下降(P < 0.05),心电图情况稍有好转。表明胎儿脐血间充质干细胞治疗心肌梗死大鼠可以促进心肌血管再生,改善心脏功能。中国组织工程研究杂志出版内容重点：干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程     

瑞舒伐他汀联合脐血间充质干细胞移植改善急性心肌梗死后心功能

BACKGROUND:In recent years, it has been a hot topic that stem cell transplantation is used to improve cardiac insufficiency after acute myocardial infarction by inducing regeneration of cardiomyocytes in the infarction regions. OBJECTIVE:To observe the effect of rosuvastatin combined with umbilical cord blood mesenchymal stem cells transplantation on rat cardiac function after acute myocardial infarction. METHODS:Forty-five Sprague-Dawley rats were enrolled to prepare myocardial infarction models by ligaturing the left anterior descending coronary artery. Then they were equivalently divided into model group, transplantation group and combination group. At 7 days after modeling, rats in the combination group were given injection of 300 μL umbilical cord blood mesenchymal stem cells (15.0×10⁸) via the tail vein and by gavage once a day for 28 days with 1 mg/kg rosuvastatin; rats in the transplantation group and model group were injected with 300 μL umbilical cord blood mesenchymal stem cell suspension through the tail veins or the same amount of LG-DMEM medium, respectively, followed by intragastrical administration of the same amount normal saline. At 5 weeks after modeling, indexes of cardiac function, level of plasma Lp-PLA2 and heat shock protein 70 in the infarction regions were detected by color Doppler ultrasound, enzyme-linked immunosorbent assay and western blot assay, respectively. In addition, pathological changes of myocardial tissues were observed using hematoxylin-eosin staining. RESULTS AND CONCLUSION:Left ventricular ejection fraction and left ventricular end-systolic pressure were significantly higher in the combination group than in the transplantation group as well as higher in the transplantation group than the model group (P < 0.05); compared with the transplantation group, left ventricular end-diastolic pressure was significantly decreased in the combination group, but significantly increased in the model group (P < 0.05); the number of cardiomyocytes in the infarction regions was significantly higher in the combination group than the other groups. Additionally, expression of heat shock protein 70 in the infarction regions was significantly increased in the combination group (P < 0.05). To conclude, rosuvastatin combined with umbilical cord blood mesenchymal stem cell transplantation can significantly improve rat cardiac function after myocardial infarction.     

不同月龄人脐带间充质干细胞移植梗死心肌的血运重建

背景：干细胞移植有利于心肌梗死后的心肌血运重建及改善心功能,HLA-G分子在免疫耐受状态的形成及维持中具有重要作用。 目的：观察不同月龄有HLA-G表达差异的人脐带间充质干细胞移植后对急性心肌梗死兔血运重建的影响。 方法：健康新西兰大白兔30只,随机数字表法分为小月龄细胞移植组、足月龄细胞移植组及对照组。建立兔心肌梗死模型后2周,将小月龄人脐带间充质干细胞和足月人脐带间充质干细胞分别标记BrdU,多点注射心肌梗死的交界区和中心区,对照组注射无血清培养基。 结果与结论：移植后4周,小月龄和足月龄细胞移植组在心肌梗死区均发现有BrdU示踪细胞,且两组梗死区心肌纤维化程度、心肌梗死面积均少于对照组(P < 0.01),两移植组间差异也有显著性意义(P < 0.05)。Ⅷ因子染色见小月龄细胞移植组毛细血管密度高于足月龄细胞移植组(P < 0.01),且两移植组与对照组比较差异也有显著性意义(P < 0.05)。提示HLA-G表达量较高的小月龄脐带间充质干细胞能更好促进梗死区血管新生,改善血运重建,有潜力成为心肌细胞移植的更理想来源。中国组织工程研究杂志出版内容重点：干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程全文链接：