糖原合成酶激酶3β过表达心肌干细胞移植治疗心肌梗死

BACKGROUND:The mechanism and effect of glycogen synthase kinase 3β (GSK-3β) in the differentiation of cardiac stem cells into cardiomyocytes are still unclear, although GSK-3β is closely related to the life activities of cells. OBJECTIVE:To investigate the changes of GSK-3β expression in the treatment of myocardial infarction in rats undergoing cardiac stem cell transplantation. METHODS:The isolation and culture of cardiac stem cells were performed in 10 neonatal rats. Lentivirus overexpressing GSK-3β or LacZ (control) was constructed and transferred into cardiac stem cells. Animal model of myocardial infarction was made in 30 Sprague-Dawley rats. Six weeks after model preparation, rat models were assigned into GSK-3β, LacZ or PBS group. GSK-3β or LacZ overexpressing cardiac stem cell solution or PBS in equal volume was injected into the rat myocardium, respectively. Four weeks after transplantation, the cardiac function and myocardial collagen production in rats were detected and compared. RESULTS AND CONCLUSION:Compared with the other two groups, the left ventricular ejection fraction was significantly higher, and the left ventricular end diastolic diameter was significantly lower in the GSK-3β group (P < 0.05). Hydroxyproline content, type I collagen mRNA, and type III collagen mRNA expression were significantly lower in the GSK-3β group than the other two groups (P < 0.05). Findings from Masson staining showed that the content of blue-stained collagen was significantly lower in the GSK-3β group than the LacZ group. Moreover, lowest myocardial infarction size was found in the GSK-3β group (P < 0.05). All these experimental findings show that GSK-3 overexpression plays a positive role in promoting the therapeutic effect of cardiac stem cell transplantation.     

骨髓干细胞移植心肌再生的研究进展

随着细胞生物工程技术和分子生物学的进展 ,利用自体骨髓干细胞移植方法来增加梗死区功能心肌细胞的数量 ,从而改善心功能成为可能。而骨髓干细胞移植心肌再生最终应用于临床作为一种成熟有效的治疗手段之前 ,尚存在大量待解决的问题。     

骨髓间充质干细胞治疗心肌梗死

心肌梗死是导致人类死亡的主要疾病之一,干细胞移植为心肌梗死的治疗提供了一种新的思路,因而成为近年来研究的热点。骨髓间充质干细胞因为不涉及伦理道德问题,而且取材方便,所以具有较好的临床应用前景。近年来有关骨髓间充质干细胞向心肌细胞分化的研究,骨髓间充质干细胞的输送方式及移行机制,移植细胞对心功能的影响以及移植转基因干细胞的治疗前景的研究有了长足的进展。     

心肌再生医学的研究进展

心血管疾病是导致人类死亡的主要疾患之一, 其中以冠心病、心肌梗死最为常见。导致心功能衰竭的主要原因是心肌梗死后心肌细胞的大量丢失及内源性修复机制的缺失,受损的心肌被瘢痕组织所替代，从而进一步导致心功能的丧失。心脏移植可以治疗严重心功能衰竭，但这一有效治疗措施受供体来源和免疫排斥反应等的限制。近年来，随着人类对干细胞认识的加深，应用心肌再生治疗心血管疾病已经成为一个新的研究热点。     

经静脉心肌超声造影对心肌梗死患者自体骨髓单个核细胞移植后心肌微循环的评价

目的利用经静脉心肌超声造影（IMCE）观察心肌梗死患者自体骨髓单个核细胞移植术前后心肌微循环的变化。方法40例急性心肌梗死患者在病情稳定后7～10d,行延迟经皮冠状动脉成形术（PCI）。随机分成骨髓细胞移植组（20例）和对照组（20例）,观察术前、术后1月、术后6月左心室舒张末内径（LVDd）,左室射血分数（LVEF）和术前及术后1个月的IMCE,测量平台期的心肌显像增大强度A,曲线上升平均斜率β及A与β之积在手术前后的变化。结果移植组LVEF由术前的（37.26±4.21）1月后上升到（54.42±5.26）（P〈0.05）;而对照组差异不显著（38.86±4.63对40.28±4.56）（P〉0.05）。LVDd移植组术后6月与术前比较差异不显著（50.23±3.42对52.48±3.26）（P〉0.05）;而对照组（50.96±2.68对64.31±3.28）（P〈0.05）心脏有扩大趋势。IMCE示手术后相关心肌节段的A、β及A.β均较术前显著增加。其中A.β（dB/s）移植组由术前的2.37±0.16（dB/s）增加到术后的15.60±0.24（dB/s）;较对照组（2.06±0.12至7.98±0.23）增加更为明显（P〈0.05）,示急性心肌梗死延迟PCI术后梗死相关节段心肌血流灌注速度和灌注量均有增加,自体骨髓单个核细胞移植组增加更明显。结论自体骨髓单个核细胞移植可改善梗死区心肌微循环,明显增加心肌血流量。     

干细胞移植与心肌细胞再生的研究进展

近 年来 ,有关急性心肌梗死后的血运重建如溶栓、冠脉介入治疗术、冠脉搭桥术等进展很快。研究表明 ,血运再通后能挽救濒死心肌、恢复冬眠心肌和顿抑心肌的功能 ,但不能修复或逆转已坏死的心肌 ,更不能促进心肌再生。灶性坏死的心肌组织在梗死后 1～ 2周开始吸收 ,并逐渐形成瘢痕 ,瘢痕组织无收缩能力 ,残留的心肌由于代偿而肥厚引起心室重构 ,最终可能发生心力衰竭。如何促进梗死区心肌组织修复 ,防止心室重构已成为亟待解决的课题。综合目前心肌生物学研究成果 ,可通过以下途径使心肌细胞增殖 :( 1)移植外源心肌细胞 ;( 2 )通过控制细胞周…     

BMP9促进植入心肌前体干细胞改善心梗大鼠心功能

目的 探讨骨形态形成蛋白9（BMP9）能否促进植入的心肌前体干细胞（CPCs）改善心梗大鼠的心功能。方法 用表达绿色荧光蛋白的AdBMP9腺病毒感染CPCs24h后,检测CPCs中BMP9的表达量。并将感染AdBMP9腺病毒的CPCs植入大鼠心肌梗死模型中,超声心动图检测左室心功能参数,Masson染色计算心脏梗死面积及观察植入细胞形态学和免疫荧光检测α-MHC及α-Actin蛋白的表达。结果 AdBMP9腺病毒感染CPCs24h后,BMP9的表达量较其CPCs本身BMP9的表达量有显著增高（P﹤0.05）。术后4周,植入的感染AdBMP9腺病毒的CPCs及未处理的CPCs均能与周围正常心肌组织融合且均有心肌特异性机构蛋白α-MHC及α-Actin的表达,但较周围正常组织表达差（P﹤0.05）。同时,植入的心肌前体干细胞可以使心肌梗死面积显著缩小（P﹤0.05）,而BMP9可以加强这一效果（P﹤0.01）。左室心功能功能参数结果与梗死面积结果相似。结论 BMP9对移植的心肌前体干细胞改善心梗大鼠心功能有促进作用。     

干细胞移植对心肌电活动的影响

背景：离子通道是心肌细胞最重要的电生理特性之一,干细胞向心肌细胞分化过程中离子通道的变化情况,以及干细胞移植到心脏后对心肌组织电生理学特性的影响是心血管疾病研究的新热点。 目的：综述干细胞移植对心肌电活动影响的现状及新进展。 方法：应用计算机检索维普、PubMed及SpringerLink数据库中2000-01/2011-05关于干细胞移植对心肌电活动影响的文章,在标题和摘要中以“骨髓间充质干细胞、胚胎干细胞、诱导多能干细胞、心肌电活动、离子通道”或“Bone marrow mesenchymal stem cells、Embryonic stem cells、Induced pluripotent stem cells、Cardiac electrical activity、Ion channels”为检索词进行检索。 结果与结论：最终选择21篇文献进行分析。目前国内外临床实验均未观察到干细胞移植产生的致命性心律失常,但一些心电活动和离子通道动力学在移植后发生改变,因此干细胞移植对心肌电生理的影响不容小觑。诱导多能干细胞不仅能够分化为心肌细胞,而且具有与心肌类似的电生理活动,在受损心肌进行再生性治疗中必将得到更加广泛的研究及应用。     

骨髓干细胞移植治疗缺血性心力衰竭

尽管药物治疗、介入治疗和外科手术治疗已取得了长足的进展,心力衰竭的发病率和死亡率仍未得到满意的控制,目前迫切需要新的方法治疗心衰,移植干细胞代替移植整个心脏成为研究的热点.1989年,美国东田纳西州大学教授高理权最先提出将自身骨骼肌干细胞移植至心脏促使坏死心肌细胞再生的设想,此后10年来大量的动物实验证实了其可行性,并发现骨髓干细胞移植不但可在宿主心肌内分化为心肌样肌细胞(cardiac- like muscle cells),而且可促进并生成新生血管.     

四肽化合物CMS030抗移植排斥作用的实验研究

目的：CMS030是从脾脏提取的四肽化合物,本研究观察CMS030对小鼠皮肤和心肌移植排斥反应的影响。方法：通过体外T淋巴细胞转化实验和混合淋巴细胞反应（MLR）实验,观察CMS030在体外的免疫抑制作用,建立小鼠皮肤移植和心肌移植模型,观察CMS030对小鼠同种移植物存活时间的影响,观察CMS030对移植受体小鼠T淋巴细胞转化和脾细胞分泌IL-2的影响。结果：CMS030在体外能够抑制T淋巴细胞转化和MLR,在体内可以明显延长小鼠皮肤移植物和心肌移植物存活时间,抑制皮肤移植受体小鼠T淋巴细胞转化能力和脾细胞分泌IL-2水平。结论：CMS030通过抑制T淋巴细胞分泌IL-2,抑制T淋巴细胞功能,有效拮抗移植排斥反应。     

静脉移植骨髓间充质干细胞对心肌病心力衰竭心功能的影响

BACKGROUND:Bone marrow mesenchymal stem cell transplantation can effectively improve decreased cardiac function caused by heart failure, but there is a lack of research about the effect in bone marrow mesenchymal stem cell transplantation on cardiac function in heart failure induced by cardiomyopathies. OBJECTIVE:To explore the effect of bone marrow mesenchymal stem cell transplantation on cardiac function in patients with cardiomyopathies accompanied by heart failure. METHODS:Totally 40 Sprague-Dawley rats were enrolled, and bone marrow mesenchymal stem cells were isolated from 10 rats, and the remaining rats were equivalently randomized into normal, model and stem cell transplantation groups. Then rats in the model and stem cell transplantation groups were given intraperitoneal injection of hydrochloric acid doxorubicin to prepare cardiomyopathy-induced heart failure models. At 7 days after modeling, the stem cell transplantation group was treated with bone marrow mesenchymal stem cells through intravenous transplantation, and the model group was treated with equal amount of DMEM medium. Four weeks later, cardiac function of each rat was detected, and the cell survival and differentiation were observed by immunofluorescence method. RESULTS AND CONCLUSION:At 4 weeks after transplantation, compared with normal and stem cell transplantation groups, the left ventricular systolic pressure and maximum rise/fall rate of left ventricular pressure were significantly decreased, but the left ventricular end diastolic pressure significantly increased in the model group (P < 0.05). And there was no significant difference between the normal group and the stem cell transplantation group (P > 0.05). High and dense fluorescence intensity was observed in the host myocardium immediately after transplantation. Subsequently, the fluorescence intensity and density decreased at 4 weeks, but the cell migration could be found, and some cells expressed cardiac troponin T. These results show that intravenous transplantation of bone marrow mesenchymal stem cells can improve cardiac function in rats with heart failure due to cardiomyopathies. Besides, the transplanted cells can survive in the host, and differentiate into cardiomyocyte-like cells.     

碱性成纤维细胞生长因子转染骨髓间充质干细胞移植治疗肺动脉高压

背景：目前正在兴起的干细胞治疗技术及基因修饰技术有可能在肺动脉高压治疗中获得独特疗效。 目的：探讨携带碱性成纤维细胞生长因子基因的骨髓间充质干细胞移植对肺动脉高压大鼠血流动力学水平的影响。 方法：选择3周龄SD雄性大鼠,于体外进行骨髓间充质干细胞培养及纯化,在腺病毒介导下实施基因转染,使碱性成纤维细胞生长因子基因成功导入骨髓间充质干细胞。将60只SD大鼠给予野百合碱腹腔注射(50 mg/kg)进行肺动脉高压模型复制,随机分成3组,其中肺动脉高压组于颈内静脉移植1 mL的L-DMEM培养基,骨髓间充质干细胞组于颈内静脉移植1 mL空腺病毒载体转染的骨髓间充质干细胞悬液,碱性成纤维细胞生长因子组于颈内静脉移植1 mL腺病毒介导下碱性成纤维细胞生长因子转染骨髓间充质干细胞悬液。 结果与结论：经3周治疗后,各组大鼠动脉血压差异无显著性意义(P > 0.05);其中碱性成纤维细胞生长因子组大鼠的肺动脉收缩期压力和平均肺动脉压明显低于肺动脉高压组、骨髓间充质干细胞组,差异有显著性意义(P < 0.05);各组大鼠右心室的肥大指数比较差异无显著性意义(P > 0.05);碱性成纤维细胞生长因子组大鼠血浆内皮素1水平明显低于肺动脉高压组、骨髓间充质干细胞组,差异有显著性意义(P < 0.05)。结果显示携带碱性成纤维细胞生长因子的骨髓间充质干细胞移植,能够改善肺动脉高压大鼠肺血管的血流动力学水平,保护机体血管的内皮细胞,并拮抗血管的收缩。中国组织工程研究杂志出版内容重点：干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程全文链接：     

移植骨髓间充质干细胞预防野百合碱诱导的肺动脉高压

背景：干细胞移植治疗肺动脉高压有一定疗效。 目的：观察骨髓间充质干细胞移植治疗肺动脉高压的效果及并探讨其作用机制。 方法：采用密度梯度离心法体外培养、纯化、扩增获得大鼠骨髓间充质干细胞,经荧光染料标记后备用。大鼠皮下注射野百合碱建立肺动脉高压模型,建模后1周将大鼠随机分为3组,干细胞移植组和肺动脉高压组大鼠皮下注射野百合碱建立肺动脉高压模型,1周后干细胞组大鼠经舌下静脉注射骨髓间充质干细胞悬液,肺动脉高压组注射等量不含干细胞的培养液,对照组皮下注射等量生理盐水。 结果与结论：移植后2周,与野百合碱诱导的肺动脉高压大鼠相比干细胞移植组血流动力学参数及右心室与体质量之比明显改善(P < 0.05);肺血管重构程度减轻(P < 0.05)。荧光显微镜下发现干细胞组移植的骨髓间充质干细胞在大鼠体内能存活至少2周,部分干细胞能转化为血管平滑肌细胞。说明静脉移植骨髓间充质干细胞能明显改善野百合碱造成的肺动脉高压大鼠肺血管和右心室结构的损伤。     

同种异体骨髓间充质干细胞移植在心肌梗死后心室重建中的作用

BACKGROUND:Myocardial infarction leads to ischemic changes in the myocardium, triggering the emergence of ventricular remodeling, which is an important cause of death. Myocardial infarction is a common disease in the middle-aged and elderly population, but autologous bone marrow mesenchymal stem cells from these patients exhibit a weak ability of proliferation and differentiation. Therefore, a positive attempt of allogeneic stem cell transplantation is required in order to obtain better therapeutic outcomes. OBJECTIVE:To explore the effect of allogeneic bone marrow mesenchymal stem cells on ventricular remodeling after myocardial infarction.   METHODS:Bone marrow mesenchymal stem cells from 10 neonatal rats and 10 adult rats were isolated, cultured and identified. Another 40 rats were randomly assigned into four groups (n=10/group): model group, neonatal rat cell transplantation group, adult rat cell transplantation group, or sham group. Animal models of myocardial infarction were made in rats in the all groups except for the sham group in which the rats were given sham operation. Rats in the two cell transplantation groups were given the corresponding cell transplantation. Four weeks postoperatively, heart function of rats was detected in each group, and cardiac tissues were taken to detect changes in collagen formation and blood vessel density in the infarct area. RESULTS AND CONCLUSION:Four weeks after surgery, rats in the model group showed significant changes in cardiac function indexes as compared with the other groups (P < 0.05), while compared with the model group, these cardiac function indexes improved in both two cell transplantation groups, but there was no significant difference between the two cell transplantation groups (P > 0.05). Meanwhile, compared with the model group, significantly decreased collagen formation and increased blood vessel density were found in both two cell transplantation groups (P < 0.05). Additionally, the vascular density of the infarct area was highest in the sham group (P < 0.05). Experimental results show that both neonatal and adult rat bone marrow mesenchymal stem cell transplantation can improve cardiac function of rats, reduce the formation of collagen in the infarct area and delay ventricular remodeling after myocardial infarction.     

血管内皮生长因子基因修饰骨髓间充质干细胞移植心肌梗死大鼠： 高压氧干预促进治疗性血管生成

背景：骨髓间充质干细胞移植入缺血心肌后梗死区周边心肌的缺血、缺氧、炎性反应、细胞凋亡等病理变化严重影响到移植骨髓间充质干细胞的存活,而高压氧能显著改善其缺氧状态。 目的：对心肌梗死模型大鼠缺血心肌局部移植血管内皮生长因子修饰的骨髓间充质干细胞,在移植后对大鼠进行高压氧干预,探讨其对骨髓间充质干细胞移植后所获得的治疗性血管生成效应的影响。 方法：将真核表达载体pcDNA3.1(-)/人血管内皮生长因子165转染大鼠骨髓间充质干细胞,再移植至心肌梗死模型大鼠的缺血区组织,移植前细胞行CM-DiI标记。移植后2周每日对大鼠行高压氧治疗干预。移植1个月后行心脏B超测量其左室射血分数,组织化学苏木精-伊红染色和Ⅷ因子染色并评价新生血管密度。 结果与结论：CM-DiI能有效标记骨髓间充质干细胞,标记率近100%。细胞移植1个月后高压氧干预的大鼠射血分数、再生血管密度较均明显增高(P < 0.05)。证实,高压氧干预能显著促进移植血管内皮生长因子基因修饰的骨髓间充质干细胞的心肌梗死大鼠所获得的治疗性血管生成作用,对心脏功能有明显改善作用。     

骨髓间充质干细胞在急性心肌梗死后心室重构中的应用☆

背景：动物实验和初期的临床研究表明,干细胞移植可以取代坏死心肌细胞、增加有功能心肌细胞的数量,改善心功能,从而为心肌梗死的治疗开辟了一条崭新的途径。 目的：观察心肌梗死后自体骨髓间充质干细胞移植及骨髓动员对心功能的影响。 方法：经猪髂前上棘抽取骨髓30 mL,培养得到骨髓间充质干细胞。15只猪分为3组,模型组仅建立心肌梗死模型,干细胞移植组在造模3 h后经冠状动脉注射骨髓间充质干细胞,干细胞动员组在造模3 h后连续5 d注射粒细胞集落刺激因子    150 μg/(kg•d)。 结果与结论：心肌梗死后8周,干细胞动员组、干细胞移植组左心室收缩、舒张末期内径都明显减小,射血分数较心肌梗死前提高,但差异无显著性意义(P > 0.05);干细胞动员组及干细胞移植组血清血管内皮生长因子水平较心肌梗死前有上升趋势(P < 0.05);干细胞动员组和干细胞移植组梗死交界区的毛细血管密度均大于模型组(P < 0.05)。提示心肌梗死后行自体骨髓间充质干细胞移植及骨髓动员均能明显改善心功能,但具体效果仍需进一步大样本实验研究。 关键词：缺血性心脏病;心力衰竭;骨髓间充质干细胞;血管再生;粒细胞集落刺激因子 doi:10.3969/j.issn.1673-8225.2012.10.009     

PTEN基因沉默骨髓间充质干细胞移植治疗急性心肌梗死

BACKGROUND:Bone marrow mesenchymal stem cell transplantation for myocardial infarction becomes popularized in recent years, but transplanted cells cannot survive and proliferate under early inflammatory reaction or local ischemia/hypoxia microenvironment, eventually hampering the therapeutic outcomes. OBJECTIVE:To investigate the therapeutic effect of PTEN-silenced bone marrow mesenchymal stem cells on acute myocardial infarction. METHODS:(1) Bone marrow mesenchymal stem cells from Sprague-Dawley rats were randomly assigned to receive no treatment, NCsiRNA transfection using Lipofectamin2000 or PTEN siRNA transfection using Lipofectamin2000. Cell growth curves were described using MTT method to detect cell cycle using flow cytometry. (2) Thirty Sprague-Dawley rats were selected to prepare myocardial infarction models that were randomized into three groups (n=10 per group): blank control, negative control and RNAi group. Six hours after modeling, bone marrow mesenchymal stem cells transfected with nothing, NCsiRNA and PTEN siRNA were respectively injected into the infarcted center of the left ventricular anterior wall in these three rat groups. After 4 weeks, all rats were subjected to cardiac function detection using echocardiography, and the survival and proliferation of bone marrow mesenchymal stem cells in the rats were observed by fluorescence microscopy. RESULTS AND CONCLUSION:Compared with the other two groups, a significant increase in the absorbance values at different culture time, the proportion of cells in S+G2 phase, and the number of bone marrow mesenchymal stem cells in the myocardial tissue was found in the RNAi group (all P < 0.05). Additionally, the left ventricular ejection fraction and left ventricular shortening fraction were significantly reduced in the RNAi group than the blank control and negative control groups at 4 weeks after cell transplantation (P < 0.05). Both in vivo and in vitro experimental findings showed that PTEN silencing could effectively improve cell survival and proliferation in the infarcted myocardium. Moreover, in the in vivo experiment, an overt improvement in rat’s cardiac function was achieved.      

丹红注射液联合骨髓间充质干细胞移植治疗大鼠脑梗死

背景：单纯骨髓间充质干细胞移植修复受损脑组织的作用并不十分理想。 目的：观察丹红注射液联合骨髓间充质干细胞移植治疗大鼠脑梗死的效果。 方法：用线栓法制备大鼠大脑中动脉阻塞模型,随机分为3组,模型组尾静脉注射PBS、丹红注射液组尾静脉注射2 mL/kg丹红注射液、联合治疗组联合注射2 mL/kg丹红注射液+2.0×10⁹ L^-1的骨髓间充质干细胞悬液,连续5 d,1次/d。 结果与结论：在骨髓间充质干细胞移植后2周,联合治疗组神经功能评分明显优于模型组及丹红注射液组(P < 0.05);移植后3周联合治疗组大鼠脑梗死体积明显小于模型组和丹红注射液组(P < 0.05);病理组织学观察也可见联合治疗组的组织损伤减轻程度大于丹红注射液组和模型组。结果可见丹红注射液联合骨髓间充质干细胞移植治疗大鼠脑梗死疗效显著,可以对脑细胞起到保护作用。     

骨髓间充质干细胞移植治疗老年性痴呆模型大鼠的行为学检测

BACKGROUND:More recently, studies have demonstrated that bone marrow mesenchymal stem cells can be induced in vitro to differentiate into neuron-like cells that are used for in vivo transplantation to repair nerve damage. OBJECTIVE:To study the effect of bone marrow mesenchymal stem cell transplantation on learning and memory ability of senile dementia rats. METHODS:Thirty male Sprague-Dawley rats were randomly divided into three groups: normal control group, stem cell therapy group and model control group. Rats in the latter two groups were used to establish animal models of senile dementia by intracranial injection of β-amyloid 1-40. Three weeks after modeling, rats were given bilateral hippocampal injection of induced bone marrow mesenchymal stem cell suspension in the stem cell therapy group, whereas no treatment was given in the normal control and model control groups. Morris water maze test was used to detect learning and memory ability of rats, and rat’s brain tissues were detected pathologically using hematoxylin-eosin staining. RESULTS AND CONCLUSION:After modeling, the escape latency was higher and the cross-platform frequency was lower in the model control group compared with the normal control group. After cell transplantation, the escape latency and cross-platform frequency were gradually shortened and increased with time, respectively. Compared with the model control group, the learning and memory abilities of rats were improved in the stem cell therapy group. The brain tissues were relatively intact in structure and exhibited less cell degeneration and necrosis in the stem cell therapy group compared with the model control group. To conclude, bone marrow mesenchymal stem cell transplantation exerts certain therapeutic effects on senile dementia by effectively improving the learning and memory ability.     

骨髓间充质干细胞移植心肌梗死大鼠的心功能

背景：前期研究发现移植的骨髓间充质干细胞能在受损心肌组织内存活和分化。 目的：进一步观察局部注射移植同种异体骨髓间充质干细胞对心肌梗死模型大鼠心功能的影响。 方法：体外培养的同种异体骨髓间充质干细胞达到一定数量(10⁶)后用BrdU标记。24只SD大鼠随机分为3组：正常对照组未行冠状动脉前降支结扎,取骨髓间充质干细胞约1 mL直接注射到冠状动脉前降支心肌的周围;假移植组：在冠状动脉前降支结扎后7 d,取等量DMEM培养液直接注射到梗死心肌的周围;骨髓间充质干细胞移植组：冠状动脉前降支结扎后7 d,取等量骨髓间充质干细胞直接注射到梗死心肌的周围;分别于移植前、移植后5周测定大鼠心功能。 结果与结论：①移植后5周假移植组最大左室收缩末压、左室内压最大(最小)变化速率均低于移植前(P < 0.01),而左室舒张末压高于移植前(P < 0.01);移植后5周骨髓间充质干细胞移植组最大左室收缩末压、左室内压最大(最小)变化速率高于移植前(P < 0.01),而左室舒张末压低于移植前(P < 0.01)。②移植后5周,骨髓间充质干细胞移植组大鼠的最大左室收缩末压、左室内压最大(最小)变化速率均高于假移植组(P < 0.01),而左室舒张末压明显低于假移植组(P < 0.01)。结果可见骨髓间充质干细胞移植可明显改善心肌梗死模型大鼠心功能。