盐酸小檗碱对人牙周膜细胞分泌单核细胞趋化蛋白1影响的体外实验

目的 研究盐酸小檗碱对体外培养的人牙周膜细胞(periodontal ligament cells,PDLC)分泌单核细胞趋化蛋白1(monocyte chemoattractant protein-1,MCP-1)的影响,旨在为利用天然药物治疗牙周病提供依据.方法 从离体牙分离牙周膜组织,体外培养人PDLC.经免疫组化鉴定后的人PDLC首先根据细胞培养液中是否含有10 mg/L脂多糖(lipopolysaccharide,LPS)分为LPS组和无LPS组(NLPS组),然后两组再依据培养液中盐酸小檗碱的终质量浓度(0、0.01、0.02、0.03 g/L)分为LPS空白对照组、LPS 1、2、3组及NLPS空白对照组和NLPS 1、2、3组.应用酶联免疫吸附测定法分别检测各组细胞培养上清液中MCP-1的含量,对多组间结果比较采用方差分析,组间两两比较采用Bonferroni t检验,以双侧P＜0.05为差异有统计学意义.结果 NLPS 1、2、3组MCP-1含量[分别为(11.33±0.16)、(11.45 ±0.53)、(11.25 ±0.14) ng/L]与NLPS空白对照组[(11.32±0.35) ng/L]相比差异均无统计学意义(P值均大于0.05)；LPS 1、2、3组MCP-1含量[分别为(38.14±5.34)、(34.15 ±3.36)、(26.13 ±2.12) ng/L]与LPS空白对照组[(58.42±1.52) ng/L]相比均显著降低,差异均有统计学意义(P值均小于0.001)；LPS 1、2、3组间两两比较差异均有统计学意义(P值均小于0.001).结论 当以LPS作为刺激因子时,盐酸小檗碱抑制体外培养的PDLC分泌MCP-1作用明显,并在一定范围内呈浓度依赖性.     

氧化苦参碱对内毒素作用下人牙周膜细胞白细胞介素-6和白细胞介素-1β mRNA表达的影响

目的 研究氧化苦参碱对内毒素(LPS)作用下人牙周膜细胞(PDLC)白细胞介素-6(IL-6)和白细胞介素-1β(IL-1β)mRNA表达的影响,探讨氧化苦参碱抑制LPS所造成的牙周炎症反应的机制.方法 体外分离及培养PDLC,实验组分别为LPS和不同质量浓度的氧化苦参碱溶液的不同组合,对照组为仅含1%FBS的DMEM培养液.采用逆转录聚合酶链反应(RT-PCR)检测IL-6和IL-1β mRNA的水平.结果 质量浓度为25 μg·mL-1 LPS能够有效地增强IL-6和IL-1β mRNA水平的表达,实验组高于对照组,差异有统计学意义,加入氧化苦参碱进行干预后实验组与对照组IL-6和IL-1β mRNA的表达无显著差异.结论 氧化苦参碱可抑制LPS所造成的PDLC IL-6和IL-1β mRNA表达.     

miR-140-5p通过NF-κB信号通路调控LPS诱导牙髓细胞炎症因子分泌的机制研究

目的:研究miR-140-5p对脂多糖(lipopolysaccharides,LPS)诱导牙髓细胞炎症因子分泌的影响和机制。方法:分离培养人牙髓细胞,分为对照组、LPS组、miR-NC+LPS组、miR-140-5p+LPS组,Realtime PCR检测细胞中miR-140-5p表达水平,ELISA法检测细胞培养上清液中IL-6、IL-1β和TNF-α含量,Western Blot检测细胞中p-IκBα、IκBα、NF-κBp65蛋白表达水平。用NF-κB信号通路激活剂处理LPS条件下转染miR-140-5p mimics的人牙髓细胞,ELISA法检测培养液上清中IL-6、IL-1β、TNF-α含量,Western Blot检测细胞中p-IκBα、IκBα、NF-κBp65蛋白表达。结果:与对照组比较,LPS组细胞中miR-140-5p水平下降,细胞培养液上清中IL-6、IL-1β、TNF-α含量升高,细胞中p-IκBα、NF-κBp65蛋白表达增多,IκBα蛋白表达没有变化。与miR-NC+LPS组比较,miR-140-5p+LPS组细胞中miR-140-5p水平升高,细胞培养液...     

茶多酚对脂多糖诱导人牙周膜成纤维细胞凋亡及炎症因子的影响

目的:探讨茶多酚对脂多糖(LPS)诱导人牙周膜成纤维细胞(HPDLFs)凋亡及肿瘤坏死因子-α-(TNF-α)、白介素6(IL-6)分泌的影响。方法:体外培养HPDLFs,MTT法筛选茶多酚浓度梯度,再分别用LPS(1μg/ml)和LPS(1μg/m1)+茶多酚(10μg/ml、50μg/ml、100μg/ml)刺激HPDLFs,流式细胞仪检测细胞凋亡,Elisa法检测TNF一α、IL-6分泌。结果:LPS可显著诱导HPDLFs凋亡,上调TNF一α、IL-6表达。茶多酚可显著降低LPS诱导的细胞凋亡和TNF-α、IL-6表达,且呈量效依赖性。结论:茶多酚可通过有效降低LPS诱导的细胞凋亡和TNF一α、IL-6表达。     

牡荆素对脂多糖诱导牙髓干细胞表达炎症因子的影响

目的：探讨牡荆素（vitexin,VTX）对脂多糖（lipopolysaccharide,LPS）诱导的人牙髓干细胞（human dental stem pulp cells,hDPSCs）表达炎症因子的影响,并初步探讨其相关作用机制。方法：分离、培养hDPSCs,以CCK-8法检测VTX对hDPSCs增殖的影响,检测VTX毒性浓度范围。铺种hDPSCs,分为4组,空白组以不含LPS和VTX的无血清DMEM,LPS组仅加入含LPS终浓度为2 μg/mL的无血清DMEM培养,VTX处理组1(V1组)加入2 μg/mL LPS+25 μmol/L VTX,VTX处理组2(V2组)加入2 μg/mL LPS + 50 μmol/L VTX。培养48 h,实时荧光定量PCR检测各组细胞中IL-1β、IL-6及IL-8基因转录,蛋白质免疫印迹检测hDPSCs内MPAK信号通路及COX-2等蛋白的变化。采用SPSS 16.0软件包对数据进行统计学分析。结果：VTX在200 μmol/L范围内时细胞活力不受影响(P>0.05);VTX抑制LPS刺激hDPSCs转录IL-1β、IL-6及IL-8,抑制效果与VTX的浓度呈正相关;VTX可显著抑制LPS激活p38及ERK信号通路。结论：VTX可能通过抑制p38及ERK信号通路的激活,降低LPS诱导的hDPSCs转录IL-1β、IL-6及IL-8。     

氧化苦参碱对内毒素作用下人牙周膜细胞白细胞介素-6和白细胞介素-1β mRNA表达的影响

目的研究氧化苦参碱对内毒素（LPS）作用下人牙周膜细胞（PDLC）白细胞介素-6（IL-6）和白细胞介素-1β（IL-1β）mRNA表达的影响,探讨氧化苦参碱抑制LPS所造成的牙周炎症反应的机制。方法体外分离及培养PDLC,实验组分别为LPS和不同质量浓度的氧化苦参碱溶液的不同组合,对照组为仅含1% FBS的DMEM培养液。采用逆转录聚合酶链反应（RT-PCR）检测IL-6和IL-1β mRNA的水平。结果质量浓度为25 μg·mL-1 LPS能够有效地增强IL-6和IL-1β mRNA水平的表达,实验组高于对照组,差异有统计学意义,加入氧化苦参碱进行干预后实验组与对照组IL-6和IL-1β mRNA的表达无显著差异。结论氧化苦参碱可抑制LPS所造成的PDLC IL-6和IL-1β mRNA表达。     

低能量激光照射对脂多糖介导人牙周膜成纤维细胞炎性损伤的保护作用

目的研究低能量激光照射(LLLI)对脂多糖(LPS)介导人牙周膜成纤维细胞(hPDLFs)炎性损伤的影响及机制。方法将hPDLFs接种于培养板内,随机分为正常组、LPS组和LPS+LLLI组。正常组细胞行常规培养基培养,LPS组及LPS+LLLI组在加入1 mg·L^-1LPS培养基中培养24 h,LPS+LLLI组3个亚组分别接受不同强度照射。4 d后检测各组细胞凋亡、活力及细胞内游离Ca²⁺浓度,酶联免疫吸附试验检测各组细胞上清液中肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-8、IL^-1β及IL-6的含量,逆转录聚合酶链反应(RT-PCR)及Western blot检测各个实验组hPDLFs的基质金属蛋白酶(MMP)-2、MMP-3、MMP-9基因和蛋白的表达。结果与正常组比较,LPS组hPDLFs细胞凋亡率及细胞内游离Ca²⁺浓度增加,细胞活力降低(P<0.05),细胞上清液中TNF-α、IL-8、IL^-1β及IL-6的含量显著升高(P<0.05),hP...     

不同培养条件对骨骼肌细胞增殖及分化的影响

目的:研究不同培养条件对骨骼肌细胞增殖及分化的影响。方法:以体外培养小鼠肌母细胞C2C12为研究模型,对照组用含φ=10%胎牛血清液培养,实验组培养基改变为φ=2%胎牛血清的DMEM液,分别于更换培养液后24、48、72h,观察细胞形态变化。结果:对照组细胞形态实验前后无变化,实验组24、48h时部分细胞出现死亡,72h存活细胞开始融合,肌管形成。结论:胎牛血清含量为φ=2%的DMEM液有促进肌母细胞分化,从而进一步形成肌管的作用;含φ=10%的DMEM不能使之分化,提示不同血清含量的DMEM培养基对体外培养的骨骼肌细胞的增殖和分化有重要影响     

富血小板血浆对人牙周组织细胞矿化作用的影响

目的观察富血小板血浆(PRP)对人牙龈成纤维细胞(GF)、牙周膜细胞(PDLC)和牙槽骨成骨细胞(AOB)的矿化诱导作用。方法抽取健康成人的新鲜全血,经过两次离心得到富血小板血浆。培养GF、PDLC和AOB,取第4代细胞用于实验,3种细胞均以4×107个/L接种于6孔培养板内,标准条件下培养96h后换不同的培养液,实验组换用含有矿化液(10nmol/L地塞米松、10mmol/Lβ-甘油磷酸钠和新鲜制备的0·2mmol/L抗坏血酸)和50ml/LPRP的DMEM(100ml/LFBS)培养液,对照组换用含有矿化液的DMEM培养液,空白组换用普通DMEM培养液。隔天换液,培养至第30天进行vonKossa染色,显微镜下观察并记录结果,用图像分析软件半定量分析染色阳性面积比及细胞衰减的面积比。结果对于PDLC和AOB,实验组及对照组的矿化量多于空白组(P<0·05),同时实验组的矿化量高于对照组(P<0·05);对于GF,3个组之间的矿化量差异无统计学意义(P>0·05)。AOB实验组在形成基质矿化时的衰减比对照组和空白组少(P<0·05)。结论PRP可促进PDLC和AOB在体外形成矿化小结,并能减少HAOB在矿化形成时的衰减,提示PRP可提高牙周骨组织的矿化和再生。     

罗红霉素对人牙周膜成纤维细胞TNF-α、IL-6释放的影响

目的:探讨罗红霉素对脂多糖诱导的人牙周膜成纤维细胞肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)释放的影响。方法:采用ELISA方法,检测在不同剂量的罗红霉素作用下,脂多糖诱导的人牙周膜细胞中IL-6、TNF-α释放的变化。结果:LPS刺激后人牙周膜细胞TNF-α的释放显著高于正常对照组(360 pg/m l vs 80 pg/m l,3 h,P<0.05)。与L组相比,罗红霉素组(20μg/m l)TNF-α水平12 h显著降低,3~6 h与L组无明显差异,而12 h(520 pg/m l vs 710 pg/m l)、24 h(220 pg/m l vs 680 pg/m l)显著降低(P<0.05)。LPS组IL-6水平显著高于正常对照组(360 pg/m l vs 105 pg/m l,P<0.05),罗红霉素组(20μg/m l)IL-6分泌量在6 h(420 pg/m l vs 670 pg/m l)、12 h(590 pg/m l vs 810 pg/m l)和24 h(340 pg/m l vs 630 pg/m l)较L组均显著降低(P<0.05)。结论:罗红霉素能显著抑制LPS刺激后人牙周膜细胞IL-6、TNF-α的释放。     

Evidence of the validity of a model of determinants of quality of restorative dental care

A model describing the relationship between self-reported quality of restorative dentistry and dentist characteristics for 119 Montana general dentists is presented. The best predictors formed a significant model explaining 22% of the variance of the quality measure. Results are contrasted with a previous estimation of the model for 102 Washington general practitioners. Evidence for the external validity of the model is presented.     

Experimental evidence of formation of imines in the course of reduction of hydrazones

The reduction of hydrazones is generally suggested to proceed through a reductive cleavage of the nitrogen–nitrogen bond followed by a reduction of the carbon–nitrogen bond. This sequence of reduction processes is here supported for fluorenone (V) and benzophenone (VI) hydrazones as well as by a comparison of the reduction of fluorenone and benzophenone hydrazonium ions (I,III) with corresponding imines (II,IV). Another proof of the presence of imines as intermediates is the splitting of four-electron waves of hydrazones V and VI and hydrazonium ions I and VIII into two waves at pH < 2. This has been interpreted as due to differences in slopes dE_1/2/dpH and pK_a-values of protonated hydrazine derivatives on one side and corresponding imines on the other. In this pH-range imines formed in reductions of VI and VIII are reduced in a single two-electron wave, those of I and V in two one-electron steps. Fluorenone imine (II) is sufficiently stable to allow recording of time-independent current–voltage curves between pH 6 and 11. In this pH-range the imine (II) is reduced in two one-electron steps. Benzophenone imine (IV) has been found stable between pH 4.6 and 12. At pH 4.6–8 the reduction of the imine IV takes place in a single two-electron step, at pH 8–12 in two one-electron steps. Final proof of the initial cleavage of the N–N bond is presented by comparison with the reduction of nitrones.     

不同剂型玻璃离子水门汀溶解性比较研究

目的:研究、比较不同剂型玻璃离子水门汀的溶解性和表面微观形态改变,为临床使用提供依据.方法:将3M树脂加强型玻璃离子水门汀(水粉剂型)、GC玻璃离子水门汀(水粉剂型)及GC玻璃离子水门汀(双糊剂型)分别在人工唾液中浸泡30 d,冷热循环15000次,烘干测重,比较前后质量变化,计算溶解率,并用扫描电镜观察表面微观改变.结果:不同剂型的玻璃离子水门汀溶解率由高到低分别为3M树脂加强型玻璃离子水门汀(水粉剂型)、GC玻璃离子水门汀(水粉剂型)、GC玻璃离子水门汀(双糊剂型).3种玻璃离子水门汀经浸泡溶解后,SEM扫描表面微观形态可观察到GE玻璃离子水门汀(双糊剂型)表面形态改变较少,其他2组玻璃离子水门汀表面微观改变较多.结论:双糊剂型玻璃离子水门汀理化性能及溶解率均低于传统水粉剂型,是未来临床修复治疗的的良好选择.     

Evaluation of efficacy of chemiluminescence for diagnosis of leukoplakia

ObjectiveLeukoplakia is the most common potentially malignant disorder preceding oral cancer. Chemiluminescence has been developed as an adjunct to conventional examination for the diagnosis of these potentially malignant disorders. This study was conducted to assess the efficacy of chemiluminescence in the diagnosis of leukoplakia and to compare the results with histopathological examination.Study designA total of 50 patients with leukoplakia were included from the outpatients attending the Department of Oral Medicine and Radiology, Dental Hospital, Bengaluru, Karnataka, India. These patients were subjected to conventional oral examination followed by chemiluminescent examination with Vizilite (Zila, Fort Collins, CO, USA) and biopsy for histopathological confirmation.ResultsThe sensitivity, specificity, positive predictive value, and negative predictive value of chemiluminescence were 93.75%, 55.56%, 78.95%, and 83.3%, respectively. The overall accuracy of chemiluminescence was 80%. A statistically significant association was observed between histopathology results and chemiluminescence results.ConclusionAlthough it is an easy, safe, minimal time consuming, and noninvasive technique, it has only adjunctive utility and it does not replace biopsy for the diagnosis of leukoplakia.     

Design of clinical trials of traditional therapies of periodontitis

The present paper on the design of clinical trials of periodontal therapy first addresses the issue of the etiology of periodontal disease. It is suggested that most if not all forms of destructive periodontal disease are caused by microorganisms and that there are different forms of disease with different microbial etiologies. The progressive nature of destructive periodontal disease is subsequently discussed and it is emphasized that, in a given patient, periodontal sites which show signs of inflammation and attachment loss may not over a period of several months and years show further sign of attachment loss. The present methods of assessing periodontal disease do not allow us to discriminate between potentially active and inactive sites in untreated patients. The significance and variability of indicators of periodontal disease such as bleeding on probing, probing pocket depth and probing attachment level measurements are discussed. The errors inherent in the various measurements are analyzed and suggestions are presented describing how alterations in any of the above parameters could be identified and presented in a clinical trial. Of concern for the statistical analysis of clinical data of periodontal disease is the definition of the "experimental unit". For a number of years, the "experimental unit" in periodontal trials was the patient. It is clear, however, that different sites within the same individual show different patterns of disease progression and lesion morphology and often respond differently to periodontal therapy. Statistical analyses must consequently be designed which recognize differences in site-to-site infection and lesion morphology within a common host. Until such analyses are available, the investigator should be wary of pooling data within the same individual, since such pooling may obscure meaningful alternatives which may take place in individual periodontal sites. Some goals of periodontal therapy are subsequently identified. 4 goals are discussed more in detail, namely: to establish conditions which will allow the patient to maintain a dentition without further breakdown of the periodontium; to reduce pocket depth to establish an anatomy in the dentogingival region which with proper maintainance care will prevent the re-establishment of the subgingival infection; to gain attachment as a result of treatment; to assess the effect of a certain chemotherapeutic agent on periodontal disease.     

颌骨动静脉畸形的栓塞治疗

目的：总结直接穿刺结合经血管内介入栓塞治疗颌骨动静脉静脉畸形的经验。方法：收治凳骨动静脉畸形患者6例,均进行了介入栓塞治疗。采用的栓塞材料为附凝血棉纤毛的螺圈,聚乙烯醇泡沫微粒和二氰基丙烯酸对丁酯。数字减影颈动脉造影在PHILIPSV300下完成。结果6例颌骨动静脉畸形患者中4,例急性出血得到了快速、有效控制,1例慢性渗血的右下 骨动静脉畸形患者,介入栓塞治疗,拔除松动的右下凳第一磨牙,有效地控制了出血,另1例伴局部软组织搏动性膨隆的上凳骨动静脉畸形患者,介入治疗后膨隆的搏动性得到明显改善,栓塞治疗后分别随访3－24个月,均未发现有口腔内渗血或出血。随访的X线片上,病灶区可见新骨形成。结论：局部穿刺结合经血管内介入栓塞治疗颌骨动静畸形是一种安全、有效的治疗方法。     

正畸患者切牙影像失真发生情况分析

目的研究正畸患者曲面体层片上的切牙影像失真发生情况，并分析其原因。 方法从中山大学附属口腔医院放射科影像数据库中选取500例正畸患者的曲面体层片和头影测量侧位片，所有曲面体层片均采用咬合杆投照，分别从切牙牙体影像放大、缩小、牙根变短、根尖模糊等评价指标分析上下颌切牙影像失真的发生情况，在头影测量侧位片上测量中切牙根尖-对颌切牙切缘的距离，探讨切牙影像失真发生的原因。采用SPSS 19.0统计软件对所得数据进行统计学检验。 结果500例患者中，切牙牙体影像正常者共417例，切牙牙体影像失真者共83例，影像失真发生率16.6%，其中切牙牙体影像放大17例、牙体影像缩小0例、牙根变短30例，牙根影像变短伴模糊36例。影像失真患者的根尖-切缘距离大于影像正常的患者，差异有统计学意义（F = 5 187.18，P = 0）；影像失真患者的覆盖值大于影像正常的患者，差异有统计学意义（F>477，P = 0）。 结论严重牙颌面畸形如反 、深覆盖是导致曲面体层片的切牙影像失真的主要原因之一。     

正常青年Monson球面半径的测量研究

目的测量正常青年Monson球面半径。方法选择60名(男30名,女30名)正常青年制取全口印模,应用立体摄影成像的原理与方法对Monson球面半径进行测量和统计学处理。结果Monson球面的半径平均为10.173 cm,大于理论值10.160 cm,差异有显著性(P<0.01);男、女性球面半径差异无显著性。结论本实验所得到的数据可作为全口义齿修复中记录颌位关系的一个参量。     

《口腔医学》杂志2007年度有关信息的分析

目的通过对《口腔医学》2007年全年文献的回顾和分析,了解我国口腔临床医学的研究现状。方法阅读2007年《口腔医学》全年的文献,对各种信息进行了分类汇总,根据设计类型对临床一次性文献进行了分类,并对其中的试验性研究文章进行质量分析。结果《口腔医学》2007年全年的一次性文献274篇,基础和临床文献分别为108和166篇,以临床文献为主(60.58%)。在临床研究文献中,属于观察性、分析性和试验性的文献分别为97、9和60篇,观察性文献所占比例为36.14%,高于以往的报道。60篇观察性文献中,全都设有对照;统计方法应用得当者44篇;真正做到随机、盲法的分别只有4和2篇。结论我国口腔临床方面的研究水平近年来提高明显,但设计的科学性方面有待提高。     

鼻测量法的进展

唇裂术后继发畸形是指唇裂修复术后,仍遗留或继发于手术操作和生长发育变化而表现出来的一类畸形[1]。包括唇畸形、鼻畸形和颌骨畸形。其修复较原发性唇裂修复更复杂,更灵活多变。而导致其修复复杂性的一个重要原因即是局部组织结构复杂变异和缺乏可靠的三维测量手段[2],鼻畸形