探针技术在中药材品质监控方面的研究与应用(英文)

ＩＮＴＲＯＤＵＣＴＩＯＮ　　Ｔｈｅｔｙｐｅａｎｄｓｐｅｃｔｒｕｍｏｆｄｉｓｅａｓｅｓａｒｅｃｈａｎｇｉｎｇｓｉｇｎｉｆｉｃａｎｔｌｙａｓｔｈｅｓｏｃｉｅｔｙａｇｉｎｇｔｏｄａｙ .Ｔｈｅａｕｔｏｉｍｍｕｎｅｄｉｓｅａｓｅｓｓｕｃｈａｓｓｅｎｉｌｅｄｅｍｅｎｔｉａ ,ＡＩＤＳ ,ａｓｗｅｌｌａｓｃａｒｄｉｏ ｃｅｒｅｂｒａｌｖａｓｃｕｌａｒｄｉｓｅａｓｅｓｓｕｃｈａｓｈｙｐｅｒｔｅｎｓｉｏｎ ,ａｒｒｈｙｔｈｍｉａ ,ｍｙｏｃａｒｄｉａｃｉｎｆａｒｃｔｉｏｎａｒｅｂｅｃｏｍｉｎｇａｎｉｎｔｒａｃｔａｂｌｅａ…     

Recent advances in pharmacologic prevention and treat ment of osteoporosis

１ＩｎｔｒｏｄｕｃｔｉｏｎＯｓｔｅｏｐｏｒｏｓｉｓｉｓａｒｅｄｕｃｔｉｏｎｉｎｂｏｎｅｍａｓｓａｎｄａｃｈａｎｇｅｉｎｍｉｃｒｏａｒｃｈｉｔｅｃｔｕｒｅ，ｗｈｉｃｈｉｎｃｒｅａｓｅｓｔｈｅｓｕｓｃｅｐｔｉｂｉｌｉｔｙｏｆｆｒａｃｔｕｒｅｓ．Ｏｓｔｅｏｐｏｒｏｓｉｓｉｓｔｈｅｍｏｓｔｃｏｍｍｏｎｍｅｔａｂｏｌｉｃｂｏｎｅｄｉｓｅａｓｅｉｎｔｈｅｄｅｖｅｌｏｐｅｄｗｏｒｌｄａｎｄｉｓｉｎｃｒｅａｓｉｎｇｌｙｒｅｃｏｇｎｉｓｅｄａｓａｎｉｍｐｏｒｔａｎｔｐｕｂｌｉｃｈｅａｌｔｈｐｒｏｂｌｅｍ．Ｏｓｔｅ…     

Selection process for investigators and study sites

ｈｅｈｉｇｈｃｏｓｔｏｆｅｆｏｒｔｓｉｎｄｏｉｎｇｃｌｉｎｉｃａｌｓｔｕｄｉｅｓｎｅｃｅｓａｒｙｔｏｅｓｔａｂｌｉｓｈｔｈｅｓａｆｅｔｙａｎｄｅｆｉｃａｃｙｏｆａｄｒｕｇｉｓｗｅｌｅｓｔａｂｌｉｓｈｅｄ．Ｉｔｉｓｈｉｇｈｌｙｄｅｓｉｒａｂｌｅｔｈａｔ...     

Journal of Chinese Pharmaceutical Sciences Instructions to Authors 2003

1 .Ｔｈｉｓｊｏｕｒｎａｌｉｓａｗｏｒｌｄｗｉｄｅｄｉｓｔｒｉｂｕｔｅｄｊｏｕｒｎａｌｏｆｐｈａｒｍａｃｅｕｔｉｃａｌｓｃｉｅｎｃｅｓｉｎｃｌｕｄｉｎｇｍｅｄｉｃｉｎａｌｃｈｅｍｉｓｔｒｙ ,ｐｈｙｔｏ ｃｈｅｍｉｓｔｒｙ ,ｐｈａｒｍａｃｏｇｎｏｓｙ ,ｐｈａｒｍａｃｅｕｔｉｃａｌａｎａｌｙｓｉｓ ,ｐｈａｒｍａｃｅｕｔｉｃｓａｎｄｐｈａｒｍａｃｏｌｏｇｙ .Ｔｈｅｊｏｕｒｎａｌｐｕｂｌｉｓｈｅｓｐａｐｅｒｓｏｆｔｈｅｆｏｌｌｏｗｉｎｇｃａｔｅｇｏｒｉｅｓ :ｏｒｉｇｉｎａｌｒｅｓｅａｒｃｈａｒｔｉｃｌｅｓ…     

Human cloning as disease prevention-human somatic cell nuclear transfer,relevance to therapy

Ｒｅｃｅｎｔｐｒｏｇｒｅｓｉｎｔｈｅｔｒａｎｓｆｅｒｏｆｓｏｍａｔｉｃｃｅｌｎｕｃｌｅｉｔｏｏｃｙｔｅｓｏｆｄｏｍｅｓｔｉｃａｎｄｌａｂｏｒａｔｏｒｙｓｐｅｃｉｅｓｈａｓｅｓｔａｂｌｉｓｈｅｄａｎｅｗｐａｒａｄｉｇｍｆｏｒｓｔｕｄｉｅｓｏｆｃｅｌｄｉ...     

锯齿缘断闻的视网膜脱离42例临床分析

Ｔｈｅ ｃａｓｅｓ ｗｅ ｒｅｐｏｒｔ ｉｓ ｔｈｅ ｓｅｒｒａｔｅｄ ｔｅａｒ ｔｈａｔ ｈｏｓｐｉｔａｌｉｚｅｄ ｄｕｒｉｎｇ ｔｈｅ ｐａｓｔ ｔｗｅｌｖｅ ｙｅａｒｓ．Ｔｈｅ ｔｏｔａｌ ｉｓ ４２ｃａｓｅｓ（４３ｅｙｅｓ）ｔｈａｔａｃｃｏｕｎｔｓ ｆｏｔ １２．５ ｐｅｒｃｅｎｔ ｏｆ ｔｈｅ ｒｅｔｉｎａ ｄｅｔａｅｈｍｅｎｔ ｉｎ ｔｈｅ ｓａｎｅ ｐｅｒｏｉｄ．Ｔｈｅ ｓｅｒｒａｔ     

The hypertension optimal study

Ｉｎｔｈｉｓｓｔｕｄｙｗｅｉｎｖｅｓｔｉｇａｔｅｄｗｈｅｔｈｅｒｔｈｅｒｅｉｓａｎｙｂｅｎｅｆｉｔｆｒｏｍｔｈｅｉｎｔｅｎｓｉｖｅｌｏｗｅｒｉｎｇｏｆｂｌｏｄｐｒｅｓｕｒｅｉｎｐａｔｉｅｎｔｓｗｉｔｈｈｙｐｅｒｔｅｎｓｉｏｎ〔１〕．Ｔｈｅａｉｍｗａｓ...     

免疫亲和毛细管电泳技术的进展

1　ＩｎｔｒｏｄｕｃｔｉｏｎＩｍｍｕｎｏａｓｓａｙ (ＩＡ ) ,ｏｎｅｏｆｔｈｅｍｏｓｔｕｓｅｆｕｌｂｉｏｍｅｄｉｃａｌｔｏｏｌｓｉｎｔｈｅｌａｓｔｆｏｕｒｄｅｃａｄｅｓ[1～ 3 ] ,ｉｓａｍｅｔｈｏｄｂａｓｅｄｏｎｔｈｅｓｐｅｃｉｆｉｃｉｎｔｅｒａｃｔｉｏｎｓｂｅｔｗｅｅｎａｎｔｉｂｏｄｉｅｓａｎｄａｎｔｉｇｅｎｓ,ａｎｄｉｓｕｓｅｄｔｏｄｅｔｅｒｍｉｎｅｄｉｆｆｅｒｅｎｔａｎｔｉｇｅｎｓ ,ｈａｐｔｅｎｓ,ｏｒａｎｔｉｂｏｄｉｅｓｉｎｃｏｍｐｌｅｘｂｉｏｌｏｇｉｃａｌｍａｔｒｉｃｅｓｂｙｔａｋｉｎｇａｎ…     

Asian randomized clinical trials in MEDLINE 1990-1997

ＴｈｅｒｅａｒｅｓｅｖｅｒａｌｒｅａｓｏｎｓｆｏｒａｎｉｎｃｒｅａｓｅｄｉｎｔｅｒｅｓｔｆｏｒｒａｎｄｏｍｉｚｅｄｃｏｎｔｒｏｌｅｄｔｒｉａｌｉｎＡｓｉａｓｕｃｈａｓａｄｉｓｔｉｎｃｔｌｏｃａｌｄｉｓｅａｓｅｐａｔｅｒｎ，ｅｔｈｎｉｃｇｒｏｕｐｄｉｆ...     

Quality of life in patients with systemic lupus erythematosus in Hong Kong

Ｓｙｓｔｅｍｉｃｌｕｐｕｓｅｒｙｔｈｅｍａｔｏｓｕｓ（ＳＬＥ）ｉｓａｈｅｔｅｒｏｇｅｎｅｏｕｓａｕｔｏｉｍｍｕｎｅｄｉｓｅａｓｅ．Ｔｈｅａｅｔｉｏｌｏｇｙｉｓｎｏｔｆｕｌｙｕｎｄｅｒｓｔｏｄｂｕｔｉｔｍａｙｉｎｖｏｌｖｅｂｏｔｈｇｅｎｅｔｉｃａｎｄｅ...     

零交导数分光光度法测定乳酸左氧氟沙星葡萄糖注射液中乳酸左氧氟沙星的含量

OBJECTIVETo determine the content Levofloxacin glucose injection. METHODA zero-crossing derivative spectrophotometry method.RESULTSThe calibration curves is linear in the range of 5.0-17.5ug.mL-1(r=0.9998)for Levofloxacin.The average recovery was 100.08%     

分光光度法测定大豆磷脂中磷的含量

研究分光光度法测定大豆磷脂中磷的含量。方法：将样品中有机磷酸化合解转化为无机磷酸盐再利用在酸溶液中磷酸盐与钼酸铵及Ｆｉｓｋｅ－ｓｕｂｂａｒｏｗ试剂用生成蓝色的化合物,在６６０ｎｍ处用分光光度法测定。结果：磷的浓度在０．０４ｕｇ／ｍｌ－０．１６ｕｇ／ｍｌ之间线性关系良好,相关系ｒ＝０．９９９９,平均回收率为１００．１％,ＲＳＤ＝０．５７％（。结论本方法简便,准确,重现性好。     

紫外分光光度法测定复方姜黄降脂片中大类成分姜黄总酚的含量

目的:建立复方姜黄降脂片中的姜黄总酚测定方法。方法:采用紫外分光光度法,在421nm测定吸收度,计算含量。结果:本方法的线性范围2.580～5.160μg/m(lr=0.9999),平均回收率为101.2(RSD=1.08%,n=5)。结论:本方法简单、准确,可用于该制剂的质量控制。     

紫外分光光度法测定氧氟沙星氯化钠注射液的含量

目的:建立氧氟沙星氯化钠注射液的含量测定方法。方法:采用紫外分光光度法。用盐酸溶液(0.1mol/L)为溶剂,检测波长293nm。结果:回归方程为A=0.0840055C+0.0033149(r=0.9999),氧氟沙星在3.2～9.2μg/mL浓度范围内呈良好的线性关系,平均回收率为99.26%,RSD为0.66%。结论:该方法简便快速、重现性好,可作为快速测定氧氟沙星氯化钠注射液含量的方法。     

二阶导数光谱法测定复方异丙嗪糖浆中盐酸异丙嗪的含量

目的用紫外分光光度法不经分离直接测定复方异丙嗪糖浆中盐酸异丙嗪的含量。方法二阶导数光谱法,测定波长为256nm。结果盐酸异丙嗪在5．21～31．26ug/ml浓度范围内线性关系良好,峰零间振幅D对浓度C的回归方程C=-651．25×D＋0．3256,r=1．0000,平均回收率为101．10％,相对标准偏差为0．98％。结论二阶导数光谱法测定复方异丙嗪糖浆中盐酸异丙嗪含量简便、快速、结果准确。     

诺氟沙星滴耳液的一阶导数分光光度测定

采用一阶导数分光光度法测定复方滴耳液中诺氟沙星的含量，样品不须分离，方法简便，结果满意。平均回收率１００．２４％，ＲＳＤ０．６３％，ｒ＝０．９９９９。     

一阶导数紫外分光光度法测定气喘合剂中氨茶碱的含量

目的：探讨气喘合剂中氨茶碱的含量测定方法。方法：采用一阶导数紫外分光光度法,测定波长为２８２ｎｍ和２８６ｎｍ。结果：氨茶碱浓度在８～１８μｇ／ｍｌ范围内线性良好（ｒ＝０９９９９）,平均回收率为９９７２％（ｎ＝５）,ＲＳＤ为０４２％。结论：本法取量少,无需化学试剂,所用仪器普通,操作简便、快速,能消除其他组分干扰。适用于医院制剂分析。     

Derivative spectrophotometry in the analysis of mixtures of cefotaxime sodium and cefadroxil monohydrate

Derivative spectrophotometry (ratio-spectra 1st- and 2nd-derivative and zero-crossing 2nd-derivative techniques) was applied for the determination of some cephalosporins in two component mixtures. Cefotaxime sodium salt (C(16)H(16)O(7)N(5)S(2)Na) and cefadroxil monohydrate (C(16)H(17)N(3)O(5)S.H(2)O) were examined. In all procedures, the calibration plots are linear up to 43 microg/ml of each antibiotic, with r ranging from 0.9997 to 0.9999. In the ratio-spectra method, the measurements were taken at 239.5 and 291.5 nm (cefotaxime, 1st-derivative), 238 and 283 nm (cefadroxil, 1st-derivative), 284 and 303 nm (cefotaxime, 2nd-derivative), and 229.5 and 245.5 nm (cefadroxil, 2nd-derivative). Detection limits at P=0.05 level of significance, calculated by a statistical treatment of calibration data, ranged from 0.15 to 0.58 microg/ml. LOD and LOQ ranged, respectively, from 0.19 to 0.51 and from 0.63 to 1.70 microg/ml. By the zero-crossing 2nd-derivative method, lines of regression are linear at 257 and 279 nm (cefotaxime) and 242 and 296 nm (cefadroxil). Detection limits from 0.28 to 0.51 microg/ml. LOD and LOQ from 0.27 to 0.41 and from 0.90 to 1.37 microg/ml, respectively. All the samples were tested for stability in solution and in the course of actual analysis, up to 80 h from their preparation. The developed derivative spectrophotometric methods were applied to synthetic mixtures and the RSD values ranged between 0.05 and 1.35% (ratio-spectra technique) and 0.01 and 1.07% (zero-crossing technique). The methods were also applied to vials and tablets for these drugs. The recoveries obtained were between 100.9 and 102.4% (ratio-spectra) and between 99.8 and 102.0% (zero-crossing). The procedures are simple, rapid, and did not require any preliminary separation or treatment of the samples. Instrumentation commonly available was utilised. The cephalosporins analysed are frequently used antibiotics of relevant clinical and pharmacological importance; hence this work would be of interest for the readers of journals devoted to pharmaceutical and biomedical analysis.     

紫外分光光度法测定达美康片的含量

本文采用紫外分光光度法直接测定达美康片剂的含量。实验结果表明：本法简便、准确、重现性好。线性范围５．０１８２５．０９μ４ｇ／ｍｌ，γ＝０．９９９９，平均回收率９９．８１％（ｎ＝５），ＲＳＤ＝１．９０％     

紫外分光光度法测定酞霜中水杨酸苯酯的含量

目的：建立测定钛霜中水杨酸苯酯含量的方法。方法采用紫外分光光度法测定水杨酸苯酯的含量。结果测定的线性范围为5～30μg/ml（R=0.9999）;方法的平均回收率为101.04%,RSD=0.51%。结论紫外分光光度法用于测定钛霜中水杨酸苯酯的含量,方法可靠,简便易行。