食管黏液表皮样癌误诊分析

2000年1月至2009年12月于我院内镜室进行检查的746例食管恶性肿瘤患者中有9例经术后病理证实为食管黏液表皮样癌.9例患者术前内镜检查均明确为食管癌,男7例,女2例;年龄25～77岁,平均为51岁;临床表现无特异性,以渐进性吞咽困难及胸骨后不适常见.     

食管黏液表皮样癌误诊分析

2000年1月至2009年12月于我院内镜室进行检查的746例食管恶性肿瘤患者中有9例经术后病理证实为食管黏液表皮样癌.9例患者术前内镜检查均明确为食管癌,男7例,女2例;年龄25～77岁,平均为51岁;临床表现无特异性,以渐进性吞咽困难及胸骨后不适常见.     

食管黏液表皮样癌误诊分析

2000年1月至2009年12月于我院内镜室进行检查的746例食管恶性肿瘤患者中有9例经术后病理证实为食管黏液表皮样癌.9例患者术前内镜检查均明确为食管癌,男7例,女2例;年龄25～77岁,平均为51岁;临床表现无特异性,以渐进性吞咽困难及胸骨后不适常见.     

涎腺黏液表皮样癌流式细胞术DNA含量检测及临床意义

目的探讨涎腺黏液表皮样癌的DNA含量、异倍体率与肿瘤组织分级关系。方法应用流式细胞术检测40例涎腺黏液表皮样癌及15例正常涎腺组织的DNA含量。结果涎腺黏液表皮样癌细胞的DNA含量、异倍体出现频率升高,且与肿瘤组织分级呈负相关,其DI值及DNA异倍体出现频率明显高于正常涎腺组织,具统计学意义（P〈0.05）。结论流式细胞术检测肿瘤细胞的DNA含量与倍体分析可用于涎腺黏液表皮样癌的诊断。     

支气管黏液表皮样癌7例

目的 探讨支气管黏液表皮样癌的临床特征、诊断和治疗方法.方法 回顾性分析7例支气管黏液表皮样癌的诊治过程,男3例,女4例,年龄15-74岁.病变部位位于左肺下叶2例,右肺上叶2例,中叶2例,右主支气管1例.结果 7例均在我院明确诊断,6例通过纤维支气管镜(纤支镜)确诊,1例通过手术确诊.所有患者进行了手术治疗,存活至今.结论 支气管黏液表皮样癌是临床少见病,该病诊断时需提高警惕,纤支镜检查是术前确诊的主要手段,手术是唯一有效的治疗方法.     

涎腺粘液表皮样癌凝集素检测意义

本文应用组织化学和免疫组织化学方法,研究6例粘液表皮样癌,9例正常涎腺作对照观察。用4种凝集素抗体(PNA、WGA、SBA 和 UEA)检测正常细胞和肿瘤细胞,结果表明,PNA 和 UEA 在细胞分布上极不一致,用于诊断涎腺粘液表皮样癌有实用意义,而癌内粘液属混合性粘蛋白,与正常腺体内粘液相比,仅含量略增高。凝集素检测本癌还可解释癌细胞发生、转化的部分原因。     

肺黏液表皮样癌一例并文献复习

目的 探讨肺黏液表皮样癌的临床病理学特征及相关病变的鉴别.方法 对1例肺黏液表皮样癌进行病理学分析及免疫组化研究,并复习相关文献.结果 镜下肿瘤成份由黏液细胞、表皮样细胞和中间型细胞构成,部分细胞排列成被覆黏液柱状上皮的腺样结构,大部分为上皮样细胞实性区.特殊染色显示黏液细胞PAS、AB-PAS染色阳性.免疫组化染色示CK（＋）、CK7（＋）、CEA（＋）、EMA（＋）、CK5/6（＋）.结论 肺黏液表皮样癌较肺其他类型恶性病变少见,其典型的组织形态有助于病理诊断,但结构不典型时需与实性黏液细胞腺癌、黏液性腺癌、腺样囊性癌或腺鳞癌鉴别.     

涎腺嗜酸性细胞腺癌3例临床病理分析

目的探讨涎腺嗜酸性细胞腺癌的临床病理特点及诊断标准以及和相关疾病的鉴别。方法对3例涎腺嗜酸性细胞腺癌进行临床病例观察并复习文献。结果 3例均有周围组织的侵犯,1例有周围淋巴结的转移,中、短期随访均未见复发。结论诊断标准主要以周围组织和脉管、神经的侵犯,其次观察细胞的异型性。治疗以手术切除为主,辅以放、化治疗效果很好,定期复查和随访也很重要。     

肺黏液表皮样癌11例临床分析

目的分析研究肺黏液表皮样癌（pulmonary mucoepidermoid carcinoma,PMEC）临床特点、治疗及预后。方法回顾我科手术治疗的11例原发PMEC患者,男性7例,女性4例。行肺叶切除7例,袖式切除＋支气管成形3例,全肺切除术1例。结果全部患者均治愈出院,无严重并发症发生。病理为高分化9例,低分化2例。术后平均随访54.20个月,1、3、5年生存率分别为100%（11/11）,85.71%（6/7）,60.00%（3/5）。1例低分化患者于术后34个月死于脑转移。结论 PMEC临床罕见且大多数肿瘤为低度恶性,多可通过完全切除肿瘤获得治愈。而高度恶性肿瘤患者预后差。     

CD44不同变异体在涎腺多形性腺瘤中的表达及意义

目的 探讨CD44不同变异体在涎腺多形性腺瘤中表达及意义.方法 应用免疫组织化学方法检测30例涎腺多形性腺瘤CD44v3、CD44v6的表达.结果 CD44v3在涎腺多形性腺瘤阳性(66.7%)表达,CD44v6在涎腺多形性腺瘤中呈强阳性表达(86.7%).结论 CD44v3、CD44v6在涎腺多形性腺瘤呈阳性表达,提示瘤细胞粘附力较强,可能是涎腺多形性腺瘤不易发生转移的原因.     

癌胚抗原相关细胞黏附分子6和增殖细胞核抗原在涎腺黏液表皮样癌中的表达及临床意义

目的 研究癌胚抗原相关细胞黏附分子6(CEACAM 6)与增殖细胞核抗原(Ki-67)在不同病理特征的涎腺黏液表皮样癌(MEC)中的表达情况及临床意义.方法 应用免疫组织化学SP法检测2005年6月至2014年10月河北北方学院附属第一医院病理科留存的50例不同临床病理特征的涎腺MEC组织、30例正常涎腺组织与30例多...     

涎腺腺样囊性癌血管内皮生长因子表达的意义

目的 探讨涎腺腺样囊性癌血管内皮生长因子表达的意义。方法 采用免疫组织化学SP法检测34例涎腺腺样囊性癌VEGF的表达。结果 VEGF表达与涎腺腺样囊性癌病理分型、淋巴结转移、临床分期有关,与发病部位、肿瘤大小无关。结论 VEGF的表达可作为判断涎腺腺样囊性癌生物学行为的一个指标。     

微波治疗涎腺粘液囊肿疗效观察

目的探讨微波治疗涎腺粘液囊肿的疗效。方法使用WB-100B型微波多功能治疗机对40例涎腺粘液囊肿患者进行治疗。术区常规消毒，用1％的卡因棉片置于囊肿表面及周围黏膜上表面麻醉后，将针状辐射探头直接插入囊腔内，进行烧灼治疗，至囊肿范围组织发白。所用微波频率为2450MHz，输出功率40W。根据囊肿大小酌情连续操作2～3次。术后常规口服抗生素3d。结果术中患者感到轻度疼痛(无需特殊处理)。术后7d复查无一感染，痂皮大部脱落，肉芽组织生长良好。15d创面愈合，口腔黏膜外形良好，无瘢痕挛缩、无畸形，随访1～2年无一复发，治愈率100％。结论微波治疗涎腺粘液囊肿，操作简便易行，疗效满意，值得推广应用。     

Immunohistochemical study on GABAergic system in salivary glands

Gamma-aminobutyric acid (GABA) and its receptors are found in the central nervous system and several peripheral tissues. The purpose of this study was to determine the expression and distribution of GABA and glutamate decarboxylase (GAD), a GABA biosynthetic enzyme, in rat salivary gland. Western blot and real time quantitative RT-PCR revealed that GAD67 was the major isoform of GAD in the salivary glands. Furthermore, both GABA and GAD were detected around the acinar cells in the submandibular glands by immunohistochemical analysis. When both sympathetic and parasympathetic nerves related to the submandibular glands were denervated, the immunoreactivities of GABA and GAD were dramatically depressed, and levels of GAD67 and GABA significantly decreased. However, no morphological changes in the glands were observed after denervation. These results indicate that GAD67 is present around acinar cells in the salivary glands, and suggest that the GABAergic system in the glands is closely related to the autonomic nervous system.     

上唇腺泡细胞癌临床分析并文献复习

目的 探讨小涎腺腺泡细胞癌的临床表现、病理特点、治疗方法和预后.方法 对1例腺泡细胞癌患者的临床资料进行总结并结合国内外已发表有关腺泡细胞癌临床和治疗特点的文献进行复习.结果 1例81岁男性患者主诉上唇肿物3年.患者3年前发现上唇黏膜长一肿物,约黄豆粒大小,无疼痛等自觉症状,未进行治疗,就诊时肿物渐增大1个月,影响上唇运动及美观就诊前1周,肿物曾自行破溃,流出血性分泌物前来就诊.入院后局麻下行上唇肿物切除术,术后病理为上唇腺泡细胞癌,局部侵犯表皮形成溃疡,未见明确脉管及神经侵犯,肿瘤未侵犯基底部横纹肌组织.结论 唇腺泡细胞癌临床极少见,术前应注意与基底细胞腺瘤和多形性腺瘤鉴别.

Abstract：

Objective To investigate the clinical and pathological characteristics of acinic cell carcinoma and to discuss its treatment and prognosis. Methods Acinic cell carcinoma (ACC) diagnosed in beijing chaoyang Hospital was retrospectively analyzed and the literature published was reviewed. Results ACC is a slow growing,low-grade malignancy of the salivary glands. Most cases occur in the parotid, but a few cases affecting the minor salivary glands have also been described, cases reported in the palate glands were more than in the labial glands. Generally think that ACC can occur at any age, but most cases occur in the elderly people and show a female predilection. The clinical feature of ACC were observed as following:indolent tumor, slow growing, long course of disease,like pleomorphic adenoma, lymp node metastasis was rare. Conclusion ACC in the labial glands is extremely rare. It's should be identified with basal cell adenoma and pleomorphic adenoma.     

定量唾液腺显像评价放射性碘治疗后唾液腺功能

目的观察分化型甲状腺癌（DTC）在首次接受不同剂量131I治疗后唾液腺功能情况。方法将40例确诊为DTC患者按照应用131I剂量不同分为两组,应用放射性核素唾液腺动态显像,对131I治疗前及治疗后3个月唾液腺功能进行定量分析。结果低剂量组（2．96GBq）治疗后仅腮腺30min摄取率（UR30）明显受损（P〈0．05）,高剂量组（5．55GBq）腮腺和颌下腺UR30、排泌分数（EF）、排泌率（ER）均明显降低,排泌时间（EP）显著延长（均P〈0．05）;腮腺较颌下腺受损更重。结论首次接受131I治疗DTC患者的唾液腺功能会受损,受损程度与使用131I剂量相关。     

Functional significance of neuronal and extraneuronal transmitter uptake in rat salivary glands

Summary The effect of normetanephrine (NM) on effector cell response to noradrenaline (NA) in vivo and on extraneuronal amine uptake in vitro has been investigated in rat submaxillayr glands. Tissue slices were incubated with ³H-NA and the metabolic pattern of the radioactivity in the slices as well as of the efflux from the slices was analyzed. The level of extraneuronally retained radioactive material was found to be markedly reduced at a NM concentration of 20 g/ml after inhibition of neuronal uptake by protriptyline (PTP). In the presence of an intact neuronal membrane pump mechanism 200 g/ml of NM also reduced the level of radioactive material, indicating that NM has an inhibitory effect on extraneuronal and in higher concentration also on neuronal uptake and retention of NA and its metabolites. NM was found to markedly potentiate the secretory response to NA in vivo, when doses of NA larger than 1 g were used. At lower NA doses there was no difference between NM pretreated and control rats. When neuronal uptake was prevented by pretreatment with PTP, NM was found to potentiate the secretory response to NA over the entire dose range. Inhibition by PTP of neuronal uptake only did not affect the dose-response curve for NA and data are presented which indicate that the absence of such a potentiation is due to a decreased salivary gland blood flow induced by NA after PTP pretreatment. The increased responses recorded after NM could not be ascribed to an additive effect of this amine, since NM was found to be a very weak -receptor agonist in the rat submaxillary gland; doses of more than 1000 g i.v. were needed to detect a secretory response. The data indicate that the extraneuronal uptake of the transmitter has a functional significance in the rat submaxillary gland by reducing high transmitter concentrations in the vicinity of the receptors.     

涎腺粘液表皮样癌p1 6癌基因蛋白表达的研究

目的：探讨p16癌基因蛋白表达与涎腺液表皮样癌分化程度的关系。方法：用超敏S－P免疫组化方法检测32例涎腺粘液表皮样癌p16癌基因蛋白的表达。结果：p16癌基因蛋白阳性表达主要见于细胞浆,阳性率为62．5％（20／32）;高分型、中分化型和低分化型肿瘤p16癌基因蛋白表达阳性率分别为86．67％（13／15）、54．55％（6／11）和16．67％（1／6）。经统计学处理,高分型与低分化型肿瘤p16癌基因蛋白表达差异有显著意义（P＜0．01）,高分化型与中分化型、中分化型与低分化型肿瘤p16癌基因蛋白表达差异无显著意义。结论：p16癌基因蛋白表达可作为判断涎腺粘液表皮样癌组织分化程度的一个参考指标。     

卵巢外腹膜浆液性乳头状癌临床病理分析

目的分析卵巢外腹膜浆液性乳头状癌（EPSPC）临床病理特点及预后。方法结合文献资料回顾性分析空军总医院收治的3例EPSPC患者的临床资料、病理切片及随访资料,并重新行免疫组化染色。结果3例EPSPC患者血清CA125均升高。肿瘤以低柱状上皮细胞排列成大小、形态不规则乳头,细胞核大,可见砂粒体。过碘酸雪夫染色阳性,免疫组化染色：肿瘤细胞CA125、S-100、CD15、广谱细胞角蛋白阳性,孕激素受体、雌激素受体、Calretinin阴性。结论EPSPC发病率低、误诊率高,进行免疫组化等病理检测有利于EPSPC的早诊断、早治疗。