孕妇,胎盘,脐带血雌二醇,睾酮及孕酮含量的测定及临床意义

孕妇、胎盘、脐带血雌二醇、睾酮及孕酮含量的测定及临床意义刘志翔李广宙张磊单智张艳华＊（附院核医学科２６１０３１＊市立二院）雌二醇（Ｅ２）、睾酮（Ｔ）及孕酮（Ｐ）广泛影响全身的代谢和功能。孕妇血清Ｅ２、Ｔ及Ｐ测定报道较多，但对孕妇、胎盘及脐带血清中其含...     

胆结石患者的性激素,血脂,血糖,胰岛素及一氧化氮的改变及临床意义

本文测定了４０例的男女胆结石患者各２０例男女正常人的雌激素（Ｅ２）、睾丸酮（Ｔ）、总胆固醇（ＴＣ）、甘油三脂（ＴＧ）、血糖（ＢＧ）、胰岛素（ＩＳ）、一氧化氮（ＮＯ）。结果显示，男女胆结石患者的Ｅ２，ＴＣ，ＴＧ，ＢＧ，ＩＳ，ＮＯ均显著高于各男女正常对比组，Ｔ均低于各正常对照组，Ｅ２与血脂，ＢＧ，ＩＳ，ＮＯ正相关，Ｔ与ＮＯ负相关，提示高Ｅ２血症、低Ｔ血症通过影响血脂，ＢＧ代谢及胆囊平滑肌松弛致胆石的形     

肺结核,慢性支气管炎,肺心病及肺癌患者血清铜,锌,镁,…

测定２９例肺结核，１７例慢性支气管炎，２０例慢性肺原性心脏病和７３例支气管肺癌患者的血清铜、锌、镁、铁含量。结果表明，血清锌、铁降低，血清铜升高，提示支气管管肺部有感染存在。铜／锌比值的显著升高，对诊断支气管肺癌有一定价值，并可作为原发性肺癌高危人群普查的检测方法之一。对感染过程中血清金属元素含量改变的机理进行了讨论。     

尿毒症患者MPV,PDW,PAgT,PAdT测定及临床意义

血小板各项参数的变化在尿毒症患者出血的发病机制中起着重要作用，积极探索尿毒症患者血小板形态、数量、大小、容积的变化及功能状况，对于临床防治尿毒症出血有着重要意义。本文对尿毒症患者血小板数量（ＰＬＴ）、血小板平均体积（ＭＰＶ）、血小板体积分布宽度（ＰＤＷ）、血小板大细胞比率（Ｐ—ＬＣＲ），及血小板聚集率（ＰＡｇＴ）和血小板粘附率（ＰＡｄＴ）进行检测分析，现报道如下。材料与方法１．实验对象　尿毒症（ＣＲＦ）组４０例，年龄３３～６５岁，其中男１９例，女２１例；正常对照组４８例，年龄２５～５８岁，均为健…     

ppm,pphm,ppb,ppt的英文全称及原义

ppm,pphm,ppb,ppt都不是量纲一的量的单位的专用名称,更不是单位符号;它们只是一种表示数量份额的英文名称的缩写,而且ppb和ppt在不同的国家代表不同的数值,如不加注释会引起歧议。因此,它们不能作单位使用。     

ppm,pphm,ppb,ppt的英文全称及原义

ppm,pphm,ppb,ppt都不是量纲一的量的单位的专用名称,更不是单位符号;它们只是一种表示数量份额的英文名称的缩写,而且ppb和ppt在不同的国家代表不同的数值,如不加注释会引起歧议。因此,它们不能作单位使用。     

ppm,pphm,ppb,ppt的英文全称及原义

ppm ,pphm ,ppb ,ppt都不是量纲一的量的单位的专用名称,更不是单位符号;它们只是一种表示数量份额的英文名称的缩写,而且ppb和ppt在不同的国家代表不同的数值,如不加注释会引起歧议。因此,它们不能作单位使用。ppm ,pphm ,ppb ,ppt的英文全称及原义缩　写全　　称原　义ppm pa     

新生儿及胚胎期股骨头骨骺及骺板中钙,铁,锌,锰,铜含量的研究

用ＸＲＦ法测定３５例无下肢疾病的死亡新生儿以及流产正常胎儿股骨头骨骺、骺板中元素钙、铁、锌、锰、铜的含量。结果表明，骺板中元素含量均高于骨骺，其中钙含量最高，高峰值在胚胎期２８周，其余４种元素的高峰值在胚胎期的２４周或２８周，阐述了发生机理，指出上述五种元素对新生儿、胚胎期胎儿正常发育有着重要意义。     

142例冠心病患者血脂及载脂蛋白AI,B100,CI,CII及CIII的研究

Serum triglycerides (TG), cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and apolipoproteins AI, B100, CI, CII and CIII were analyzed in 142 patients with coronary heart disease (CHD) and 64 age-matched healthy subjects in the Chengdu area. Significantly increased TG and decreased HDL-C were found in the CHD patients (P less than 0.001). Serum apo AI and CII were significantly decreased in the CHD patients compared with the normal subjects (P less than 0.01 or P less than 0.001). Serum apo B100 and CIII were slightly higher in the CHD group than in the normal group (P less than 0.001 or P less than 0.05). No significant differences were observed in apo CI levels in the CHD patients when compared with the normal subjects. The results were discussed.     

高糖及高脂膳食对血浆载脂蛋白AⅠ,AⅡ...

Effects of high carbohydrate (CHO) and high fat diet on plasma apolipoproteins (apo) AI, AII, B100, CI, CII, CIII and E were assessed in 10 healthy subjects and 10 patients with endogenous hypertriglyceridemia. High CHO diet consisted of 80% CHO, 5% fat and 15% protein of total calories, and high fat diet consisted of 40% CHO, 45% fat and 15% protein. After 5 days normal diet (60% of total calories from CHO, 25% from from fat and 15% from protein), the subjects studied received sequentially high CHO diet and normal diet for 5 days and high fat diet and normal diet for 5 days. Fasting blood samples were drawn on days 1, 3, and 5 of high CHO and high fat diet period, and 5 days after taking normal diet. Results showed that fasting plasma apo AI slightly decreased (P less than 0.01) on day 3 of the high CHO diet period and slightly increased (P less than 0.01) on day 3 of the high fat diet period, plasma apo AII increased after intake of high CHO diet, but no changes were observed after the subjects were placed on high fat diet. Fasting plasma apo B100 decreased (P less than 0.001) in hypertriglyceridemics on the 3rd day of the high CHO diet, but no differences were noted in normal subjects on the same diet. On the 3rd day of the high fat diet period, apo B100 increased (P less than 0.01) in normal subjects, but no changes were found in hypertriglyceridemics. Apo CII and CIII levels declined during the high CHO diet period but increased in normal subjects after intake of high fat died.(ABSTRACT TRUNCATED AT 250 WORDS)     

人脑脊液载脂蛋白AI,B100,CII CIII及E的测定

Apolipoproteins AI, B100, CII, CIII and E contents were measured by radial immunodiffusion assay kits developed by our Research Unit. The apolipoprotein in 10 human cerebrospinal fluid samples was determined. Results show that there are no apo B100, CII or trace apo CIII in human cerebrospinal fluid, the levels of apo AI in cerebrospinal fluid are 1.00 +/- 0.54 mg/dl with a range of 0.35-2.00 mg/dl and the apo E content is 0.69 +/- 0.16 mg/dl with a range of 0.51-0.99 mg/dl. The results are discussed.     

载脂蛋白AⅠ,CⅠ,CⅡ,CⅢ,E对肝细胞膜高密度脂蛋白...

Effects of purified human apolipoproteins AI, CI, CII, CIII-1, Cs and E on the binding of 125I-labeled apo E-deficient HDL3 to isolated rat liver plasma membranes were investigated. Unlabeled apo AI, CI, CII, CIII-1 and apo Cs, but not apo E, could effectively inhibit 125I-labeled HDL3 binding to liver membranes, apoCIII-1, was the strongest among the apoC subclasses and the inhibition curve of apoCIII-1 was similar to that of apo AI. This result indicates that apo C, especially apo CIII, may be another specific ligand of HDL receptor, and it plays an important role in regulation of HDL receptor activity in liver.     

脂质代谢、尿酸与突发性聋的关系

目的探讨突发性聋与血清脂质代谢、血尿酸之间的关系。方法回顾性分析突发性聋住院患者100例的病史特点、血液学检查和影像学检查结果,检测其血清中甘油三酯、胆固醇、高密度胆固醇、低密度胆固醇、载脂蛋白AI、载脂蛋白B、血尿酸的含量。结果突发性聋组高密度胆固醇低于对照组,血尿酸值高于对照组,且差异有统计学意义（P〈0.05）,各年龄组之间的高密度胆固醇、尿酸值差异无统计学意义（P〉0.05）。两组之间的甘油三酯、胆固醇、低密度胆固醇、载脂蛋白AI、载脂蛋白B值均差异无统计学意义。结论高密度胆固醇下降、尿酸代谢紊乱是突发性聋发生的危险因素。     

慢性阻塞性肺病对脂蛋白(a)水平的影响

目的观察慢性阻塞性肺疾病(COPD)患者脂蛋白(a)[Lp(a)]水平是否同COPD合并冠心病的高发生率相关.方法检测90名COPD患者及与之年龄、性别、吸烟史等相近的90例健康人的血脂水平.结果同健康对照组相比,COPD患者的Lp(a)及载脂蛋白B-100(ApoB100)水平显著下降,且两者水平呈明显相关性,治疗用药及是否吸烟对ApoB100影响不大.结论COPD患者易患冠心病的原因与Lp(a)的高低关系不确切,而与患者血液粘稠度升高相关.     

高脂血症时血清二乙基对硝基苯磷酸酯酶活性的变化

目的 :研究tritonWR 13 3 9致小鼠高脂血症时血清二乙基对硝基苯磷酸酯酶 (ParaoxonaseI ,PON 1)活性的变化及PON 1活性与血浆脂质含量之间的关系。方法 :在小鼠尾静脉注射tritonWR13 3 9后的 3h ,6h ,12h ,2 4h ,48h ,72h ,眼球摘除术取血 ,用酶标法测定小鼠血浆总胆固醇 (TC) ,甘油三酯 (TG ) ,高密度脂蛋白 -胆固醇 (HDL -C) ,载脂蛋白AI (ApoAI) ,载脂蛋白B (ApoB含量 ) ,并计算出ApoAI/ApoB ,HDL -C/TC比值 ,用分光光度计法测定PONI活性。结果 :TritonWR 13 3 9尾静脉注射后 3h ,小鼠血浆TC和TG含量即急剧增高 ,与对照组相比 ,P <0 .0 1,分别在注射后 2 4h和 3h达到峰值 ,随后逐渐下降 ,其中血浆TG含量在注射后 48h即恢复正常水平。tritonWR13 3 9尾静脉注射后 3h ,小鼠血浆HDL -C和ApoB含量急剧增高 ,与对照组相比 ,P <0 .0 5,分别在注射后 2 4h和 6h达到峰值 ,随后逐渐下降 ,分别在注射后 48h和 72h恢复正常水平。tritonWR13 3 9尾静脉注射后 3h ,小鼠血浆ApoAI含量即急剧降低 ,与对照组相比 ,P <0 .0 5,随后逐渐增高 ,在注射后 6h即恢复正常水平。tritonWR 13 3 9尾静脉注射后 3h ,小鼠血浆ApoAI/ApoB和HDL-C/TC比值减少 ,与对照组相比 ,P <0 .0 1,随后逐渐增高 ,其中ApoAI/ApoB比值在 72h恢复     

正常妊娠孕妇血清载脂蛋白A_1和B含量的探讨

本文采用单向免疫扩散法对正常非孕妇女(37例),正常妊娠期妇女(188例)在高征患者(24例)和原发性高血压(6例)测定了ApoA_1和ApoB。结果表明,正常妊娠ApoA_I和ApoB含量随孕周增加而升高,32周后迅速升高;妊高征患者和原发性高血压患者ApoA_I和APoB含量明显高于正常对照组(P<0.01)。认为测定妊高征患者ApoA_I和ApoB含量可做为了解血管病变程度及指导治疗的指标。     

内源性高甘油三酯血症者血浆脂蛋白中脂质和载脂蛋白...

Lipids and apolipoproteins in plasma VLDL, IDL, LDL, HDL2 and HDL3 isolated by one-step density gradient ultracentrifugation from 7 subjects with endogenous hypertriglyceridemia (HTG) and 4 normalipemics were determined. HTG VLDL was enriched with cholesterol, and its triglyceride (TG), phospholipid (PL) and apo CII, CIII contents (mg/100 mg protein) were significantly higher than those in normal VLDL. ApoE content in HTG VLDL was prone to increasing, and apoB100 content had no apparent difference from that in normal VLDL. Compared with normal HDL2 and HDL3, the contents of total cholesterol (TC), apoAI, CII, and CIII in HTG HDL2, and apoCII, CIII in HTG HDL3 decreased. HTG HDL2 and HDL3 were rich in TG. By analysis of distribution of these lipids and apolipoproteins in various plasma lipoproteins, it was found that the abnormal composition of VLDL and HDL in HTG seemed to be a result of transport of TC, PL, and apoCII, CIII from HDL to VLDL. There was a significantly positive correlation between TC and apoAI, CII, CIII in HDL respectively, which indicated that apoCII and CIII were also associated with the alteration of HDL cholesterol. It is suggested that the elevated plasma VLDL-TG concentration in endogenous hypertriglyceridemia may induce abnormal redistribution of lipid and apolipoprotein among apoCII plasma lipoproteins, and and CIII may play an important role in these changes.     

人血清载脂蛋白E火箭电泳测定法

Apo E was purified from the very low density lipoprotein (VLDL) of normal and hypertriglyceridemic subjects by Sephadex G-75, heparin-Sepharose 4B affinity chromatography, and preparative SDS polyacrylamide gel electrophoresis. The antisera were produced by injecting apo E into goats. With apo E antisera, Rocket immuno-electrophoretic method was developed to quantitate apo E levels in normal and hypertriglyceridemic human serum. The detection limit was 5.5 ng apo E. The coefficients of variation of intra- and interassay were 2.6% and 5.2%, respectively. The serum apo E level of 50 normal lipodemic subjects in Chengdu area was 4.7 +/- 1.2 mg/dl.