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1.
目的 探讨Wistar大鼠海马生后发育过程中,活化的Caspase-3与凋亡之间的关系. 方法 应用免疫荧光方法观测活化的Caspase-3和赫斯特荧光染料33342(Hoechst 33342)在生后不同时期大鼠海马CA1、CA3区和齿状回(DG)中的表达情况. 结果 在CA1区,活化的Caspase-3的表达在生后7d(P7)达到高峰;在CA3区,P2达到高峰,然后逐渐减弱.在DG,P7后又有所增强,到P14达到高峰,并在所观测的其余时段维持此水平.观测的3个区的凋亡细胞数目都在P7达到高峰,然后逐渐减少. 结论 在大鼠海马生后发育过程中,活化的Caspase-3的表达存在特定的时空格局.活化的Caspase-3在CA1区与CA3区有丝分裂后期细胞和DG神经前体细胞中的作用和机制不同.  相似文献   

2.
目的探讨Ki-67/caspase-3鸡尾酒抗体在前列腺腺癌(PAC)、高级别前列腺上皮内瘤(HGPIN)及良性前列腺增生(BPH)组织中的表达及意义。方法收集40例PAC、30例HGPIN和BPH标本,按常规方法石蜡包埋,制作蜡块连续切片,进行HE染色及Ki-67/caspase-3鸡尾酒抗体双重免疫组化染色。结果 Ki-67表达阳性率及增殖指数在PAC组织中明显高于HGPIN和BPH组织,且随着Gleason分级增高其增殖指数存在递增趋势(P0.05,P0.01)。caspase-3在BPH组织中表达阳性率明显高于PAC及HGPIN组织(P0.05),但在PAC与HGPIN、不同Gleason分级PAC中的表达阳性率差异无统计学意义(P0.05)。Ki-67和caspase-3在PAC和HGPIN组织中的表达呈负相关,而在BPH中的表达呈正相关。结论 Ki-67/caspase-3在不同病变中表达不同,鸡尾酒抗体联合检测可用于前列腺良恶性疾病的诊断、鉴别诊断及预后的判断。  相似文献   

3.
目的 探讨Wistar大鼠生后海马发育过程中钙/钙调蛋白依赖性蛋白激酶Ⅱ(CaMKⅡ)的表达.方法 应用免疫荧光方法检测CaMKⅡ在生后不同时期大鼠海马CA1、CA3区和齿状回(DG)中的表达情况(n=48). 结果 CaMKⅡ于生后各期海马CA1区和DG的表达逐渐增强,生后第10天(P10)达高峰期,此后逐渐减弱;于CA3区的表达在P4和P10时均较高.其中,CaMKⅡ在CA3区的表达高于在CA1区和DG的表达,在多形层和分子层的表达高于在锥体细胞层或颗粒细胞层的表达. 结论 CaMKⅡ在CA1、CA3区和DG中的表达具有特异性的时空分布模式,这可能与其在生后发育过程中的突触发生,树突、轴突形成,海马的成熟以及学习记忆功能相关.  相似文献   

4.
目的 探讨Wistar大鼠生后海马发育过程中钙/钙调蛋白依赖性蛋白激酶Ⅱ(CaMKⅡ)的表达。 方法 应用免疫荧光方法检测CaMKⅡ在生后不同时期大鼠海马CA1、CA3区和齿状回(DG)中的表达情况(n =48)。结果 CaMKⅡ于生后各期海马CA1区和DG的表达逐渐增强,生后第10天(P10)达高峰期,此后逐渐减弱;于CA3区的表达在P4和P10时均较高。其中,CaMKⅡ在CA3区的表达高于在CA1区和DG的表达,在多形层和分子层的表达高于在锥体细胞层或颗粒细胞层的表达。结论 CaMKⅡ在CA1、CA3区和DG中的表达具有特异性的时空分布模式,这可能与其在生后发育过程中的突触发生,树突、轴突形成,海马的成熟以及学习记忆功能相关。  相似文献   

5.
NR1、NR2A与PSD-95在生后大鼠海马发育中的表达   总被引:1,自引:0,他引:1  
目的:探讨N-甲基-D-天冬氨酸受体亚单位1(N-methy1-D-aspartate receptor subunit1,NR1)、N-甲基-D-天冬氨酸受体亚单位2A(N-methy1-D-aspartate receptor subunit2A,NR2A)与突触后密度蛋白-95(Postsynapticdensity protein 95,PSD-95)在Wistar大鼠海马生后发育过程中的表达。方法:应用免疫荧光染色方法检测NR1、NR2A与PSD-95在生后不同时期大鼠海马CA1、CA3区和齿状回(DG)中的表达情况。结果:NR1于生后各期海马CA1、CA3区和DG的表达均增强,P14~P21达高峰期后减弱。NR2A于生后在海马CA1和CA3区的表达略有减弱,P4后增强至高峰期P14,然后轻微减弱,在DG中NR2A的表达生后略有减弱,P4后在增强的趋势中于P7、P14和P28后均有不同程度的减弱,高峰期在P28。PSD-95于生后各期海马CA1、CA3区和DG的表达均增强,P21~P28达高峰期后轻微减弱。结论:NR1、NR2A和PSD-95在CA1、CA3区和DG中的表达具有特异的时空分布模式,此模式可能与其在生后发育中发挥的不同生理功能相关。  相似文献   

6.
目的:探讨caspase-12介导的内质网通路凋亡相关蛋白在大鼠肾发育中的表达规律。方法:应用免疫组织化学显色与免疫印迹法,对大鼠肾发育中内质网通路凋亡相关蛋白caspase-12和cleaved-caspase-3的表达进行定性和定量观察。结果:免疫组织化学显示在大鼠肾发育中,caspase-12和cleaved-caspase-3的阳性表达由肾皮质区深层逐渐扩展到整个肾皮质区,其表达主要位于近端小管,而远端小管表达较微弱;免疫印迹结果显示在肾发育中,caspase-12被分解为cleavedcaspase-12,caspase-12和cleaved-caspase-12的蛋白表达在胚胎期较高,在生后(P)1 d达到高峰,随后其表达逐渐降低。而cleaved-caspase-3的蛋白表达在胚胎肾发育中较弱,在P1d其表达水平突然升高,随后维持在较高的水平。结论:Caspase-12介导的内质网通路凋亡相关蛋白可能参与大鼠胚胎肾发育中近端小管的细胞凋亡。  相似文献   

7.
目的探讨Livin在乳腺癌表达及其与caspase-3蛋白表达和细胞增殖的相互关系。方法采用SP法免疫组化和原位杂交技术检测Livin在正常乳腺组织(14例)、乳腺增生性病变(14例)、乳腺非浸润性癌(20例)和乳腺浸润性癌组织(52例)中Livin mRNA和其蛋白的表达以及caspase-3、Ki-67在乳腺癌组织中的表达。结果Livin蛋白和mRNA在正常乳腺组织、乳腺增生性病变、乳腺非浸润性癌和乳腺浸润性癌组织中阳性率分别为(7.1%、7.1%)、(28.6%、28.6%)、(65.0%、70.0%)、(73.1%、75.0%),差异有统计学意义(P〈0.01)。Livin蛋白与mRNA表达之间差异无统计学意义(P〉0.05)。Livin表达与临床分期、淋巴结转移和年龄有关(P〈0.05),与肿瘤大小和组织学分级无关。Livin蛋白与caspase-3的在乳腺癌表达呈负相关(P〈0.01)。Livin蛋白表达阳性的乳腺癌Ki-67增殖指数(47.32%±22.34%)明显高于Livin蛋白表达阴性的乳腺癌Ki.67增殖指数(18.01%±23.34%)(P〈0.01)。结论Livin基因及蛋白在乳腺癌中表达上调,提示在乳腺癌发生、发展中起重要作用,可作为乳腺癌新的分子标记物,可能成为乳腺癌诱导凋亡治疗的新靶点。Livin蛋白通过与执行型caspase-3结合,抑制其活性,从而阻止细胞凋亡。Livin不仅参与细胞凋亡的调控,还促进了细胞增殖及肿瘤的发生、发展。  相似文献   

8.
目的探讨Beclin1过表达骨肉瘤组织中Ki-67、P53的表达及相关性。方法采用回顾性研究方法,选取我院诊治的骨肉瘤患者70例,所以患者均经免疫组化检测Beclin1的表达,根据Beclin1是否阳性表达进行分组比较,同时检测组织Ki-67、P53的表达,分析Beclin1过表达骨肉瘤中Ki-67、P53的表达差异性和相关性。结果在成骨性骨肉瘤、成纤维性骨肉瘤和成软骨性骨肉瘤3种骨肉瘤中Beclin1的表达差异无统计学意义(P0.05),总阳性率为62.86%,在Beclin1阳性组织中,P53的表达阳性率为52.27%,Ki-67表达阳性率为43.18%,与Beclin1阴性组比较差异具有统计学意义(P0.05);进一步相关分析显示,Beclin1的过表达与P53呈正相关(r=0.653,P=0.001),而与Ki-67呈负相关(r=-0.741,P=0.001)。结论自噬基因Beclin1过表达骨肉瘤组织中Ki-67、P53的表达状态存在差异性,Beclin1过表达状态可能与下调Ki-67而抑制细胞增殖和促进P53的表达上调而促进细胞凋亡有关,最后对肿瘤发挥抑制作用。  相似文献   

9.
目的 探讨不同剂量异氟烷全身麻醉对新生大鼠认知功能的影响及可能机制。方法 24只出生后7 d SD大鼠,随机分为对照组、1.0%异氟烷处理组、1.5%异氟烷处理组和2.0%异氟烷处理组。各组大鼠于21d后行旷场实验,35d后行Morris水迷宫检测。HE染色观察各组大鼠海马组织病理学改变;Western blot方法检测大鼠海马组织Ki-67、神经元核心抗原(NeuN)增殖、NLRP3和caspase-1蛋白的表达。结果 与对照组相比,1.0%、1.5%或2.0%异氟烷处理组大鼠海马Ki-67和Neu N蛋白表达均明显降低(P<0.05),异氟烷麻醉各组间无显著性差异。与对照组相比,1.0%、1.5%或2.0%异氟烷处理后,大鼠逃避潜伏期、目标象限时间和穿越平台次数均明显延长;大鼠海马发生一定程度的损伤;大鼠海马组织NLRP3、caspase-1、IL-1β和ASC蛋白表达升高(P<0.05)。结论 异氟烷全身麻醉可降低新生大鼠学习能力,其机制可能与激活NLRP3和caspase-1信号通路有关。  相似文献   

10.
目的观察小鼠海马发育过程中凋亡相关基因caspase-9的表达变化。方法取出生后1d、3d、7d、14d、21d和28d的小鼠海马,应用免疫组织化学技术、免疫印迹技术及图像分析技术检测caspase-9的表达变化。结果 P1 d海马CA区锥体细胞层和齿状回颗粒细胞层caspase-9阳性表达产物平均光密度最高,P3 d~P21 d caspase-9阳性表达产物平均光密度逐渐降低,P28表达水平最低。结论凋亡相关基因caspase-9在生后小鼠海马发育过程表达水平呈逐渐降低的趋势。  相似文献   

11.
The aim of the present study was to estimate the relationship between apoptosis and cell proliferation in histiocytic necrotizing lymphadenitis (HNL). Fifteen patients with HNL were retrospectively analyzed. The patients were divided into three groups according to the proportion of the necrotic area as follows: necrosis (+), necrotic area <25%; necrosis (++), necrotic area 25–50%; and necrosis (+++), necrotic area >50%. Immunohistochemical double staining was performed for CD3 plus caspase-3 and for Ki-67 plus caspase-3 and positive cells were counted in two areas: one without and one with obvious apoptotic features. Most caspase-3-positive cells were also stained for CD3 (area exhibiting obvious apoptotic features: average, 92.3%). Furthermore, various proportions of both Ki-67- and caspase-3-positive cells were detected in all the groups (range, 5–70%). In the area with obvious apoptotic changes, the average percentage of both Ki-67- and caspase-3-positive cells (38.6%) was higher than that in the area without obvious apoptotic features (16.3%). A proportion of cells in HNL undergo proliferation and apoptosis simultaneously, such as neoplastic cells, thereby exhibiting rapid cell cycles.  相似文献   

12.
13.
The distribution of the Ki-67, bcl-2 and caspase-3 proteins was immunohistochemically analyzed in the developing human upper jaw (5th-10th gestational weeks).During this period, proliferative activity gradually decreased from higher levels at the earliest stages (50-52%) to lower levels, both in the jaw ectomesenchyme and in the epithelium. The highest expression of bcl-2 protein was found in the epithelium and ectomesenchyme of areas displaying lower rates of cell proliferation. High levels of caspase-3 protein were detected during the earliest stages of jaw development, indicating an important role for apoptosis in morphogenesis of early derivatives of the maxillary prominences. The number of Ki-67, bcl-2 and caspase-3 positive cells changed in a temporally and spatially restricted manner, coincidently with upper jaw differentiation. While apoptosis might control cell number, bcl-2 could act in suppression of apoptosis and enhancement of cell differentiation. A fine balance between cell proliferation (Ki-67), death (caspase-3) and cell survival (bcl-2) characterized early human upper jaw development. A rise in the number of apoptotic cells always temporally coincided with the decrease in number of surviving bcl-2 positive cells within the palatal region. Therefore, the upper jaw development seems to be controlled by the precisely defined expression of genes for proliferation, apoptosis and cell survival.  相似文献   

14.
目的探讨Caspase-3 mRNA在小鼠肾小体发育中表达的变化特征及其可能的生物学意义。方法选取胚龄14d、16d、18d胎鼠和出生后1d、3d、7d的仔鼠肾组织,采用原位杂交技术及体视学分析方法对Caspase-3 mRNA在不同发育阶段肾小体中的表达进行定性及定量观察。结果Caspase-3 mRNA在小鼠肾小体发育的不同阶段都有阳性表达。在Ⅰ期和Ⅱ期肾小体的表达较低,进入Ⅲ期肾小体时,其表达达高峰,Ⅳ期肾小体中的表达稍有下降,Ⅴ期肾小体中的表达显著降低,其体积密度分别为12.35±3.78(%)、15.63±4.05(%)、43.28±6.09(%)、33.45±5.32(%)和10.28±2.56(%)。结论Caspase-3 mRNA在小鼠不同发育阶段肾小体内的表达不同,它对肾小体的正常发育可能有重要意义。  相似文献   

15.
We have previously demonstrated transient increases in caspase-3 activity in the hippocampus of rat pups from age 17 days. We report here our studies on the effects of inhibition of caspase-3 during this period on the acquisition of a two-way avoidance reaction. Rat pups received intracerebroventricular doses of the caspase-3 inhibitor Z-DEVD-FMK On postnatal day 18. Control animals of the same age received the inactive peptide Z-FA-FMK or isotonic saline solution. Inhibition of caspase-3 during the period of its natural activation in the hippocampus during early ontogenesis was found to impair the development of operant behavior in rats. This was apparent as a reduction in the efficiency of learning during acquisition of active avoidance reactions and decreases in the numbers of intersignal reactions. Administration of the inhibitor had no specific action on the types of conditioned reflex activity less associated with operant learning. Thus, there were no differences between the experimental and control groups in the numbers of emotional reactions to the conditioned stimulus. The number of orientational-investigative conditioned reactions also showed no change after administration of Z-DEVD-FMK. On the background of the reduction in the efficiency of the acquisition of the conditioned active avoidance reflex, the number of incomplete acts, in contrast to other types of conditioned reactions, increased significantly after administration of Z-DEVD-FMK, which is evidence for the persistence of the ability to form associative connections between activation of the conditioned signal and the need to move to the other sector. The difficulty in these animals arose at the decision-taking stage on choosing the appropriate form of behavior. Changes in orientational-investigative behavior were not associated with inhibition of caspase-3 during the critical period of development, as the effects of Z-DEVD-FMK and Z-FA-FMK were similar. Translated from Zhurnal Vysshei Nervnoi Deyatel'nosti imeni I. P. Pavlova, Vol. 57, No. 6, pp. 702–711, November–December, 2007.  相似文献   

16.
为了研究体外培养大鼠胚胎脊髓神经细胞损伤前后的凋亡变化及caspase-3的表达情况,本实验建立了一个模拟脊髓横断损伤后脊髓组分-原代培养的脊髓神经细胞机械损伤模型,并用Hoechst33342/PI双染法检测脊髓神经细胞的凋亡变化,用免疫荧光染色方法检测caspase-3的表达。结果显示:(1)损伤前几乎未见凋亡的神经细胞,损伤后6 h凋亡细胞开始出现,损伤后12 h明显增多,1 d时达高峰,但损伤后3 d凋亡细胞开始呈明显下降的趋势,至损伤后7 d又开始增加,一直持续到损伤后14d;(2)相应时刻caspase-3表达趋势的改变与脊髓神经细胞凋亡趋势的变化基本相同;(3)经过caspase-3抑制剂Ac-DEVD-CHO干预后,凋亡细胞的数量与caspase-3的表达均减少,并呈剂量依赖性。以上结果提示,机械损伤可以诱发体外培养的脊髓神经细胞凋亡,且此凋亡的发生可能与caspase-3的表达有关。  相似文献   

17.
Apoptin基因通过激活caspase-3诱导人黑色素瘤细胞A375凋亡   总被引:1,自引:1,他引:1  
目的研究caspase-3在肿瘤特异性凋亡基因诱导人黑色素瘤细胞A375凋亡中的作用。方法用含有apoptin基因的真核表达载体瞬间转染体外培养的人黑色瘤细胞A375;采用RT-PCR、DNA凝胶电泳、流式细胞术检测A375细胞的凋亡;以比色法检测caspase-3的相对活性。结果Ap-optin基因瞬间转染的A375细胞可出现典型的细胞凋亡所具有的DNA梯状带;流式细胞术发现实验组细胞凋亡率明显高于其他各组(P<0.01);转染后24h实验组caspase-3的活性开始升高,72h达高峰,明显高于其他各组(P<0.01)。结论Apoptin基因可通过激活caspase-3诱导人黑色素瘤细胞A375凋亡。  相似文献   

18.
The purpose of the present paper was to examine the level of apoptosis and the relationships among apoptosis, apoptosis-associated proteins, and proliferating potential in lymphoma tissues to clarify the characteristics of apoptosis in diffuse large B-cell lymphomas (DLBCL) of the central nervous system (CNS). The formalin-fixed, paraffin-embedded tissues of CNS and non-CNS DLBCL (20 cases each) were studied by terminal deoxynucleotidyl transferase-mediated dUTP-nick end labeling (TUNEL) and immunohistochemistry, using antibodies against single-stranded DNA (ssDNA), cleaved caspase-3, bcl-2, bax, p53, Fas and Ki-67. The cleaved caspase-3 immunohistochemistry detected apoptosis of the lymphoma cells most sensitively compared to TUNEL and ssDNA immunohistochemistry. High expression (grade + + or + + +) of cleaved caspase-3 was found more frequently in CNS DLBCL (11 cases, 55%) than non-CNS DLBCL (three cases, 15%; P = 0.009). Bax-positivity of lymphoma cells was increased in six cases of CNS DLBCL, which also showed high positivity of cleaved caspase-3. There was no significant correlation between the cleaved caspase-3-positivity and the Ki-67 positivity. The present study indicates that the number of apoptotic cells and expression level of cleaved caspase-3 were significantly higher in CNS DLBCL than non-CNS DLBCL, and that the correlation of bax and cleaved caspase-3 expression was often present in CNS DLBCL.  相似文献   

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