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本文介绍了牛黄解毒片中蒽醌甙及大黄酸的含量测定方法。(1)用比色法测定蒽醌甙和大黄酸混合成分的含量,以1,8—二羟基蒽醌为参比物,0.5%醋酸镁甲醇溶液色剂,测定波长为515nm。(2)用双波长薄层扫描法测定了总大黄酸的含量,波长λs=430nm,λR=610nm。 相似文献
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Analysis of rhubarb anthraquinones and bianthrones by microemulsion electrokinetic chromatography 总被引:2,自引:0,他引:2
In this work a method of microemulsion electrokinetic chromatography (MEEKC) has been developed for the analysis of nine anthraquinones and bianthrones in rhubarb. This study employed di-n-butyl tartrate as oil substance to make up the microemulsion. The composition of the microemulsion was 0.5% (w/w) di-n-butyl tartrate, 0.6% (w/w) SDS, 1.2% (w/w) 1-butanol and 97.7% (w/w) 10 mM sodium borate buffer, pH of the buffer being 9.2. Acetonitrile was added to the emulsion to improve the separation. The volume ratio between the emulsion solution and acetonitrile of an optimized separation was 70:30. With the optimized conditions all of the nine analytes were baseline-separated in peaks of good shapes within 20 min. After validation the method was used to analyze the components in a rhubarb sample. A solid-phase extraction procedure was employed. Five anthraquinones and two bianthrones had been detected in the sample and their amounts were determined. The method should be able to be used for the quantitative analysis of the main active components of rhubarb crude drugs. 相似文献
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植物药中一些主要成分测定方法的研究 Ⅲ.蒽醌的測定方法 总被引:5,自引:0,他引:5
本文在Paris等人所报导方法的基础上,研究了蒽醌显色后的稳定性情况,以及用酸水解结合蒽醌与提取的条件,进而分析了几种植物药中游离蒽醌与结合蒽醌的含量,认为方法尚可应用.游离蒽醌系将生药样品在Soxhlet提取器内用氯仿提取,提取液用5%NaOH-2%NH40H提取后比色,以1,8-二羟蒽醌为标准;结合蒽醌则先用5N硫酸水解,回流2小时,再加氯仿回流提取数次,至提尽为止,合并氯仿液,同上以碱液提取后比色测定. 相似文献
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目的:建立一测多评法测定复方黄白胶囊中大黄蒽醌类成分的方法。方法:以复方黄白胶囊为研究对象,以大黄酚作为内参物,计算大黄酸、大黄素和大黄素甲醚与大黄酚的相对校正因子;分别采用一测多评法和外标法测定复方黄白胶囊中上述4种大黄蒽醌类成分的含量。结果:大黄酸、大黄素及大黄素甲醚与大黄酚间的相对校正因子分别为0.946、1.105和1.097,相对校正因子重现性良好。复方黄白胶囊中大黄成分采用校正因子计算的含量值与外标法实测值之间无显著差异。结论:该方法可用于复方黄白胶囊中大黄蒽醌类成分的定量分析。 相似文献
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黄芩对大黄蒽醌在提取精制过程中转化的影响 总被引:2,自引:2,他引:0
目的探讨黄芩对大黄中5种蒽醌类成分在提取精制过程中相互转化的影响。方法 HPLC测定药材和提取物中5种成分的含量,比较药材和提取物中芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚的相对比例;再分别用5种蒽醌的对照品加入和不加黄芩药材提取液模拟提取精制过程,考察5种蒽醌成分之间的转化情况。结果大黄药材和提取物中5种成分的相对比例有明显变化,大黄酸在提取物中的比例增高;同时采用对照品模拟提取精制过程时各个成分并无转化,而加入黄芩药材提取液后,大黄酸经过提取精制处理后能部分转化为大黄素,其余4种成分之间无转化。结论黄芩和大黄药材配伍提取可以使大黄蒽醌类成分发生转化。 相似文献
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Cuizhe Liu Xigang Liu Jiming Tong Kaishun Bi 《Pharmaceutical development and technology》2013,18(5):506-515
San-huang dispersible tablet (SHDT) was designed with a patented technology to enrich the active ingredients in rhubarb and with a wide selection of excipients in the new manufacturing procedure. The total rhubarb anthraquinones were first enriched in the extract by our patented technology. Eudragit L100, S100 and PEG-6000 were used to release a part of the total rhubarb anthraquinones at the colon to induce the cathartic effect of the anthraquinones by another patented technology. Microcrystalline Cellulose (MCC), low-substituted hydroxypropyl cellulose (L-HPC), sodium carboxymethyl starch (CMS-Na), and hydroxypropyl methylcellulose (HPMC) were used to ensure quick release of baicalin and berberine hydrochloride in the stomach. The dissolution of SHDT was evaluated by a method in 2005 Chinese Pharmacopoeia along with San-huang tablet (SHT), and the results demonstrated that the dissolution of baicalin and berberine hydrochloride more than double that of SHT and release of half of the rhubarb anthraquinones in colon. 相似文献
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薄层扫描法测定男炎消颗粒中盐酸小檗碱的含量 总被引:1,自引:0,他引:1
目的: 建立薄层扫描法测定男炎消颗粒中盐酸小檗碱的含量.方法: 样品经处理后,以苯-醋酸乙酯-甲醇-异丙醇-氨水(6∶3∶1.5∶1.5∶0.3)为展开剂,测定波长λ=365 nm.结果: 此方法线性关系良好,平均回收率为98.3%,RSD=3.15%.结论: 该方法简便、灵敏、准确,可用于该制剂的质量控制. 相似文献