共查询到20条相似文献,搜索用时 15 毫秒
1.
Lea R. Chioca Marcelo M. Ferro Irinéia P. Baretta Sara M. Oliveira Cássia R. Silva Juliano Ferreira Estela M. Losso Roberto Andreatini 《Journal of ethnopharmacology》2013
Ethnopharmacological relevance
Lavandula angustifolia (lavender) inhalation has been used in folk medicine for the treatment of anxiety, and clinical and animal studies have corroborated its anxiolytic effect, although its mechanism of action is still not fully understood.Aims of the study
The objective of the present study was to determine whether the GABAA/benzodiazepine complex or serotonin neurotransmission mediates the anxiolytic-like effect of lavender essential oil.Materials and methods
Male Swiss mice were subjected to the marble-burying test after being exposed to the aroma of lavender essential oil (1–5%), amyl acetate (5%; used as a behaviorally neutral odor), or distilled water for 15 min via inhalation. Additionally, the effect of 5% lavender essential oil was also evaluated in mice subjected to the elevated plus maze. GABAA/benzodiazepine mediation was evaluated by pretreating the mice with the GABAA receptor antagonist picrotoxin before the marble burying test and [3H]flunitrazepam binding to the benzodiazepine site on the GABAA receptor. Serotonergic mediation was studied by pretreating the mice with O-methyl-[3H]-N-(2-[4-(2-methoxyphenyl)-1-piperazinyl]ethyl)-N-(2-pyridinyl) cyclohexanecarboxamide trihydrochloride (WAY100635), a serotonin 5-HT1A receptor antagonist before the marble burying test. We also evaluated changes in the pharmacologically induced serotonin syndrome and the effects of combined administration of subeffective doses of lavender essential oil and the 5-HT1A receptor agonist 8-hydroxy-2-(di-n-propylamino)tetralin (8-OH-DPAT).Results
Lavender essential oil (1–5%) decreased the number of marbles buried compared with the control and amyl acetate groups. In the elevated plus maze, 5% lavender essential oil inhalation increased the percentage of time spent on and number of entries into the open arms compared with controls. No effect was seen in the number of closed arm entries or number of beam interruptions in the automated activity chamber. Pretreatment with the GABAA receptor antagonist picrotoxin (0.5 mg/kg) did not modify the behavioral effect of 5% lavender essential oil in the marble-burying test. Lavender essential oil also did not alter [3H]flunitrazepam binding to the benzodiazepine site on the GABAA receptor. Pretreatment with the serotonin 5-HT1A receptor antagonist WAY100635 (3 mg/kg) blocked the anxiolytic-like effect of lavender essential oil and the 5-HT1A receptor agonist 8-OH-DPAT (3 mg/kg). A combination of ineffective doses of 8-OH-DPAT (0.5 mg/kg) and lavender essential oil (0.1%) reduced the number of marbles buried. Finally, 5% lavender essential oil attenuated the serotonin syndrome induced by 40 mg/kg fluoxetine plus 80 mg/kg 5-hydroxytryptophan.Conclusions
These results indicate an important role for the serotonergic system in the anxiolytic-like effect of lavender essential oil. 相似文献2.
Petra Krejčová Petra Kučerová Gary I. Stafford Anna K. Jäger Roman Kubec 《Journal of ethnopharmacology》2014
Ethnopharmacological relevance
Persian shallot (Allium stipitatum) is a bulbous plant native to Turkey, Iran and Central Asia. It is frequently used in folk medicine for the treatment of a variety of disorders, including inflammation and stress. Antiinflammatory and neurological activities of pyrithione and four related sulfur-containing pyridine N-oxides which are prominent constituents of Allium stipitatum were tested.Methods
The antiinflammatory activity was tested by the ability of the compounds to inhibit cyclooxygenase (COX-1 and COX-2), whereas the neurological activities were evaluated by assessing the compounds ability to inhibit monoamine oxidase-A (MAO-A) and acetylcholinesterase (AChE). The compounds? affinity for the serotonin transport protein (SERT) and the GABAA–benzodiazepine receptor were also investigated.Results
2-[(Methylthio)methyldithio]pyridine N-oxide showed very high antiinflammatory effects which are comparable with those of common pharmaceuticals (IC50 of 7.8 and 15.4 µM for COX-1 and COX-2, respectively). On the other hand, neurological activities of the compounds were rather modest. Some compounds moderately inhibited AChE (IC50 of 104–1041 µM) and MAO-A (IC50 of 98–241 µM) and exhibited an affinity for the SERT and GABAA–benzodiazepine receptor.Conclusions
Our findings may help to rationalize the wide use of Persian shallot for the treatment of inflammatory disorders. 相似文献3.
Ana Carla C.S. Carvalho Dayse S. Almeida Marcélia G.D. Melo Sócrates C.H. Cavalcanti Rosilene M. Marçal 《Journal of ethnopharmacology》2009
Ethnopharmacological relevance
Erythrina velutina is traditionally used for sleepiness, convulsions and nervous system excitation in Brazil. Although central effects have been reported for Erythrina velutina, little is known about its mechanism of action.Aim of the study
To investigate the pharmacological evidences of mechanism of action of Erythrina velutina leaves aqueous extract (AE).Materials and methods
Terminal segments of the guinea-pig ileum (n = 5–8) were mounted in an organ bath and isotonic contractions were recorded. Phytochemical screening was carried out on AE.Results
AE (0.025–2.50 mg/ml) produced contractile response in the guinea-pig ileum, yielding typical concentration–response curves (EC50 = 0.63 mg/ml). Electrically evoked contractions were significantly increased in the presence of AE. AE-elicited contractions were significantly reduced by bicuculline, tetrodotoxin, atropine, verapamil or incubation in low calcium–high potassium solution. Atropine along with verapamil abolished AE contractile response. Alkaloids, catechins, steroids, flavonols, flavononols, flavonoids, phenols, saponins, tannins, triterpenoids, and xanthones were detected in AE.Conclusions
AE contains important constituents for pharmacological activities. AE-induced contractions seem to involve GABAA receptor activation, acetylcholine release, muscarinic receptor activation, augmentation of Ca2+ entry through L-type calcium channels, and calcium release from the intracellular stores. These findings provide further support for Erythrina velutina traditional uses. 相似文献4.
Jobin Mathew Smijin SomanJayanarayanan Sadanandan Cheramadathikudyil Skaria Paulose 《Journal of ethnopharmacology》2010
Aim of the study
Gamma-aminobutyric acid A receptors are the principal mediators of synaptic inhibition in striatal neurons and play an important role in preventing the spreading of seizures through the striatum. In the present study, effect of Bacopa monnieri (L.) Pennel and its active component bacoside-A on spatial recognition memory deficit and alterations of GABA receptor in the striatum of epileptic rats were investigated.Materials and methods
Total GABA and GABAA receptor numbers in the control and epileptic rats were evaluated using [3H]GABA and [3H]bicuculline binding. GABAAα1, GABAAα5, GABAAγ3 and GABAAδ gene expressions were studied. Behavioral performance was assed using Y-maze.Results
Scatchard analysis of [3H]GABA and [3H]bicuculline in the striatum of epileptic rats showed significant decrease in Bmax compared to control. Real-Time PCR amplification of GABAA receptor subunits such as GABAAα1, GABAAα5 and GABAAδ, were down regulated (p < 0.001) in the striatum of epileptic rats compared to control. Epileptic rats have deficit in Y-maze performance. Bacopa monnieri and bacoside-A treatment reversed these changes to near control.Conclusion
Our results suggest that decreased GABA receptors in the striatum have an important role in epilepsy associated motor learning deficits and Bacopa monnieri and bacoside-A has a beneficial effect in the management of epilepsy. 相似文献5.
Ethnopharmacological relevance
Astragaloside IV, a major component extracted from the roots of Astragalus membranaceus (AM), possesses anti-inflammatory, anti-oxidative, anti-fibrotic, anti-infarction and immunoregulatory effects. To clarify anti-stress effect of AM, anxiolytic and anti-inflammatory effects of 80% ethanol extract of AM and astragaloside IV were investigated in immobilization stress model.Materials and methods
The mice were orally administered with AM (50, 200, and 500 mg/kg), astragaloside IV (5, 10, and 20 mg/kg) and buspirone, a positive drug, 1 h before immobilization treated for 2 h. For anxiolytic activity assay, EPM test was performed in mice. For anti-inflammatory activity assay, serum levels of corticosterone, IL-6 and TNF-α were measured using ELISA kits.Results
AM extract and astragaloside IV increased dose-dependently time spent on open arms and open arm entries in the EPM test. Anxiolytic effects of AM extract (500 mg/kg) and astragaloside IV (20 mg/kg) were comparable to those of buspirone (1 mg/kg). Their anxiolytic effects were blocked by WAY-100635 (0.5 mg/kg, i.p.), a 5-HT1A receptor antagonist (p<0.01), but not by flumazenil (3 mg/kg, i.p.) and bicuculline (0.5 mg/kg, i.p.), GABAA receptor antagonists. AM extract and astragaloside IV also reduced serum levels of corticosterone, IL-6 and TNF-α dose-dependently.Conclusions
AM, particularly astragaloside IV, may ameliorate immobilized stress-induced anxiety and inflammation. 相似文献6.
Qiuhai Qian Wenhui Chen Wang Yue Zhihong Yang Zhantao Liu Weibin Qian 《Journal of ethnopharmacology》2010
Ethnopharmacological relevance
Xiao-Ban-Xia-Tang (XBXT), a traditional Chinese herbal medicine, has been used in China for more than 2000 years, and proved to be effective on various cases of vomiting in the clinic.Objective
To investigate the antiemetic effect of XBXT on cisplatin-induced acute and delayed emesis and its effective mechanism on Neurokinin-1 receptor (NK1-R) in the new vomiting model of minks.Materials and methods
Minks were randomly divided into the normal group, cisplatin group, cisplatin + ondansetron group, cisplatin + low-dose XBXT group and cisplatin + high-dose XBXT group. The antiemetic effect of drugs was investigated in the vomiting model of minks induced by cisplatin (6 mg kg−1, i.p.) in 72 h observation, and the expression of NK1-R in the area postrema and ileum was measured by Western blot.Results
The frequency cisplatin induces retching and vomiting was significantly reduced by pretreatment with XBXT in a dose-dependent manner during the 0–24-h and 24–72-h periods (P < 0.05), and XBXT exhibited effective dose-dependent (P < 0.05) inhibition on the increase of expression levels of NK1 receptor in both the ileum and area postrema.Conclusions
XBXT has good activity against cisplatin-induced acute and delayed emesis in minks possibly by inhibiting central or peripheral increase of NK1-R. 相似文献7.
Miguel Carro-Juárez Juan Francisco Rodríguez-Landa María de Lourdes Rodríguez-Peña María de Jesús Rovirosa-Hernández Francisco García-Orduña 《Journal of ethnopharmacology》2012
Ethnopharmacological relevance
Cihuapatli is the Nahuatl name assigned to some medicinal plants grouped in the genus Montanoa, where Montanoa frutescens (Family: Asteraceae, Tribe: Heliantheae) is included. The crude extract from these plants has been used for centuries in the Mexican traditional medicine as a remedy for reproductive impairments and mood disorders. Experimental studies have systematically corroborated the traditional use of cihuapatli on reproductive impairments and sexual motivation, however, the effect on mood and “nervous” disorders, remains to be explored.Materials and methods
The anxiolytic-like effect of aqueous crude extract of M. frutescens (25, 50 and 75 mg/kg) was investigated in male Wistar rats evaluated in the elevated plus-maze and compared with several doses of diazepam (1, 2 and 4 mg/kg) as a reference anxiolytic drug. Picrotoxin (1 mg/kg), a noncompetitive antagonist of the GABAA receptor, was used in experimental procedures to evaluate if this receptor could be involved in the anxiolytic-like effects produced by M. frutescens. To discard hypoactivity, hyperactivity, or no changes associated with treatments, which could interfere with the behavioral activity in the elevated plus-maze, rats were subjected to the open field test.Results
M. frutescens at 50 mg/kg showed anxiolytic-like activity similarly to 2 mg/kg of diazepam, without disrupts in general motor activity. The anxiolytic-like effect of M. frutescens detected in the elevated plus-maze was blocked by picrotoxin, indicating that GABAA receptors are involved in the modulation of this effect.Conclusions
The results corroborate the use of M. frutescens in folk Mexican ethnomedicine as a potential anxiolytic agent and suggest that this effect is mediated by the GABAA receptors. Additionally, some sedative effects with high doses of M. frutescens were detected in the present study. 相似文献8.
Uwe Koetter Marilyn Barrett Svenja Lacher Aliaa Abdelrahman Deanne Dolnick 《Journal of ethnopharmacology》2009
Ethnopharmacological relevance
Magnolia officinalis Rehder and Wilson [Magnoliaceae] bark and Ziziphus spinosa (Buhge) Hu ex. Chen. [Fam. Rhamnaceae] seed have a history of use in traditional Asian medicine for mild anxiety, nervousness and sleep-related problems.Aim of the study
To identify pharmacological targets, extracts of Magnolia officinalis (ME), Ziziphus spinosa (ZE), and a proprietary fixed combination (MZE) were tested for affinity with central nervous system receptors associated with relaxation and sleep.Methods
In vitro radioligand binding and cellular functional assays were conducted on: adenosine A1, dopamine (transporter, D1, D2S, D3, D4.4 and D5), serotonin (transporter, 5-HT1A, 5-HT1B, 5-HT4e, 5-HT6 and 5-HT7) and the GABA benzodiazepine receptor.Results
Interactions were demonstrated with the adenosine A1 receptor, dopamine transporter and dopamine D5 receptor (antagonist activity), serotonin receptors (5-HT1B and 5-HT6 antagonist activity) and the GABA benzodiazepine receptor at a concentration of 100 μg/ml or lower. ME had an affinity with adenosine A1 (Ki of 9.2 ± 1.1 μg/ml) and potentiated the GABA activated chloride current at the benzodiazepine subunits of the GABA receptor (maximum effect at 50 μg/ml). ME had a modest antagonist action with 5-HT6 and ZE with the 5-HT1B receptor.Conclusion
The interactions in the receptor binding models are consistent with the traditional anxiolytic and sleep-inducing activities of Magnolia officinalis bark and Ziziphus spinosa seed. 相似文献9.
Ethnopharmacological relevance
Hemerocallis citrina, a traditional herbal medicine, has been used for the improvement of emotions in Eastern-Asia countries.Aim of the study
Herein, we explored the antidepressant-like effect and its monoaminergic mechanism of the ethanol extracts from Hemerocallis citrina (HCE).Materials and methods
Effect of HCE (90, 180 and 360 mg/kg, p.o.) on the immobility time was assessed in the mouse forced swim test (FST) and tail suspension test (TST), and locomotor activity was evaluated in the open-field test (OFT). Additionally, the monoamine neurotransmitters serotonin (5-HT), noradrenaline (NA) and dopamine (DA) levels involved in the antidepressant-like effect of HCE were also measured in the mice brain regions of frontal cortex and hippocampus.Results
HCE (90, 180 and 360 mg/kg, p.o.) administration significantly reduced the immobility time in both the FST and TST without accompanying changes in locomotor activity in the OFT. The pretreatment of mice with WAY 100635 (0.1 mg/kg, s.c., a 5-HT1A receptor antagonist), cyproheptadine (3 mg/kg, i.p., a 5-HT2 receptor antagonist), prazosin (62.5 μg/kg, i.p., an α1-adrenoceptor antagonist), yohimbine (1 mg/kg, i.p., an α2-adrenoceptor antagonist), propranolol (5 mg/kg, i.p., a β-adrenoceptor antagonist) or sulpiride (50 mg/kg, i.p., a dopamine D2 receptor antagonist), but not SCH23390 (0.05 mg/kg, s.c., a dopamine D1 receptor antagonist) prevented the antidepressant-like effect of HCE (360 mg/kg, p.o.) in the TST. In addition, HCE enhanced 5-HT and NA levels in the frontal cortex and hippocampus as well as elevated DA levels in the frontal cortex.Conclusion
The results indicate that the antidepressant-like effect of HCE is dependent on the serotonergic (5-HT1A and 5-HT2 receptors), noradrenergic (α1-, α2- and β-adrenoceptors) and dopaminergic (D2 receptor) systems as well as the elevation of 5-HT, NA and DA levels in the mouse brain. 相似文献10.
Rosalie Awad Fida Ahmed Natalie Bourbonnais-Spear Martha Mullally Chieu Anh Ta Andrew Tang Zul Merali Pedro Maquin Francisco Caal Victor Cal Luis Poveda Pablo Sanchez Vindas Vance L. Trudeau John T. Arnason 《Journal of ethnopharmacology》2009,125(2):257-264
Ethnopharmacological relevance
The Q’eqchi’ Maya possess a large selection of plants to treat neurological disorders, including epilepsy and susto (fright), a culture-bound illness related to anxiety disorders.Aim of the study
To investigate the activity of antiepileptic and anxiolytic plants in the GABAergic system, and determine if there is a pharmacological basis for plant selection.Materials and methods
Ethanol extracts of 34 plants were tested in vitro for their ability to inhibit GABA-transaminase (GABA-T) or bind to the GABAA-benzodiazepine (BZD) receptor, two principal drug targets in epilepsy and anxiety. Pharmacological activity was correlated with relative frequency of use, based on informant consensus.Results
Ten plants showed greater than 50% GABA-T inhibition at 1 mg/ml, while 23 showed greater than 50% binding to the GABAA-BZD receptor at 250 μg/ml. Piperaceae, Adiantaceae and Acanthaceae families were highly represented and active in both assays. There was a significant positive correlation between GABA-T inhibition and relative frequency of use for epilepsy, and an even stronger correlation between GABAA binding and relative frequency of use for susto (fright).Conclusions
Clearly, Q’eqchi’ traditional knowledge of antiepileptic and anxiolytic plants is associated with the use of pharmacologically active plants. Based on the evidence, it is suggested that the mechanism of action for some traditionally used plants may be mediated through the GABAergic system. 相似文献11.
Gisele Zapata-Sudo Thaiana C.F. Mendes Marcelle A. Kartnaller Tiago O. Fortes Nathália F.B. Freitas Maria Auxiliadora Coelho Kaplan Roberto Takashi Sudo 《Journal of ethnopharmacology》2010
Ethnopharmacological relevance
Dorstenia arifolia is a plant that has been used in the folk medicine to produce hypnotic, sedative and ansiolitic effects but the pharmacological properties have not yet been studied. In addition, the smoke of its rhizome is reputed to induce lethargic sensation.Aims of the study
The present study investigated possible activities of the methanol extract (ME) of Dorstenia arifolia rhizome on the central nervous system (CNS).Materials and methods
ME was tested for sedative, hypnotic and anticonsulsant effects using locomotor activity evaluation, pentobarbital-induced sleeping time and pentylenetetrazol (PTZ)-induced convulsion, respectively.Results
Intraperitoneal administration of ME (10 and 50 mg/kg) significantly decreased locomotor activity from 205.2 ± 25.6 movements/min (DMSO) to 112.1 ± 18.4 (P < 0.05) and 114.9 ± 16.9 (P < 0.05), respectively. Flumazenil (10 mg/kg), an antagonist of GABAA receptor, prevented the ME-induced sedation. Treatment with ME (50 mg/kg) significantly increased the duration of pentobarbital-induced sleeping time from 41.0 ± 2.3 to 57.9 ± 2.9 min (P < 0.05). The latencies to seizures after intraperitoneal injection of PTZ was recorded and compared between groups. ME promoted a significant protection of PTZ-induced seizures and mortality in a dose-dependent manner.Conclusions
Our findings indicate that ME of Dorstenia arifolia rizhome has pronounced central effects, and that the sedative and anticonvulsant activities may be related to a facilitation of the GABAergic transmission. 相似文献12.
13.
Baretta IP Felizardo RA Bimbato VF dos Santos MG Kassuya CA Gasparotto Junior A da Silva CR de Oliveira SM Ferreira J Andreatini R 《Journal of ethnopharmacology》2012,140(1):46-54
Ethnopharmacological relevance
Achillea millefolium L. (Asteraceae), known as yarrow (“mil folhas”), has been used as folk medicine for gastrointestinal disorders, inflammation, anxiety, and insomnia.Aim
To evaluate the potential anxiolytic-like effect of hydroalcoholic extract of Achillea millefolium L. in animal models.Methods
The present study evaluated the effects of the hydroalcoholic extract from the aerial parts of Achillea millefolium L. in mice subjected to the elevated plus-maze, marble-burying, and open-field tests. Additionally, the GABAA/benzodiazepine (BDZ) mediation of the effects of Achillea millefolium was evaluated by pretreatment with the noncompetitive GABAA receptor antagonist picrotoxin and the BDZ antagonist flumazenil and by [3H]-flunitrazepam binding to the BDZ site on the GABAA receptor.Results
Achillea millefolium exerted anxiolytic-like effects in the elevated plus-maze and marble-burying test after acute and chronic (25 days) administration at doses that did not alter locomotor activity. This behavioral profile was similar to diazepam. The effects of Achillea millefolium in the elevated plus-maze were not altered by picrotoxin pretreatment but were partially blocked by flumazenil. Furthermore, Achillea millefolium did not induce any changes in [3H]-flunitrazepam binding.Conclusion
The results indicate that the orally administered hydroalcoholic extract of Achillea millefolium L. exerted anxiolytic-like effects that likely were not mediated by GABAA/BDZ neurotransmission and did not present tolerance after short-term, repeated administration. 相似文献14.
15.
16.
Juliana A.T. Werner Sara M. Oliveira Daniel F. Martins Leidiane Mazzardo Josiane de F.G. Dias Ana L.L. Lordello Obdúlio G. Miguel Luiz Fernando Royes Juliano Ferreira Adair R.S. Santos 《Journal of ethnopharmacology》2009
Ethnopharmacological relevance
Geissospermum vellosii is a tree widely found throughout the Amazonic forest and frequently used by the native population for painful disorders.Aim of the study
The present study examined the antinociceptive effects of Geissospermum vellosii in behavioral models of nociception.Materials, methods and results
Oral administration of crude extract of Geissospermum vellosii or its dichloromethane fraction (1–100 mg/kg) inhibited formalin-induced inflammatory nociception and acetic acid-induced visceral nociception. The antinociceptive effect of Geissospermum vellosii was unrelated with motor dysfunctions. Furthermore, the alkaloid 12-metoxy-1-methyl-aspidospermidine (0.001–1 mg/kg), isolated from the dichloromethane fraction, also produced antinociception. The antinociception caused by the dichloromethane fraction was significantly attenuated by pre-treatment of mice with p-chlorophenylalanine methyl ester (PCPA, an inhibitor of serotonin synthesis, 100 mg/kg once a day for 4 consecutive days) and WAY-100635 (a 5-HT1A receptor antagonist, 0.3 mg/kg). In contrast, dichloromethane fraction antinociception was not affected by pre-treatment of animals with ketanserin (a 5-HT2 receptor antagonist, 0.3 mg/kg) or ondansetron (a 5-HT3 receptor antagonist, 0.5 mg/kg).Conclusions
Together, these results indicate that Geissospermum vellosii produces antinociception through an interaction with 5-HT1A receptors. Furthermore, the alkaloid 12-metoxy-1-methyl-aspidospermidine contributes to the antinociceptive properties reported for Geissospermum vellosii. 相似文献17.
Taehun Lee Sooryun Lee Kyeong Ho Kim Ki-Bong Oh Jongheon Shin Woongchon Mar 《Journal of ethnopharmacology》2013
Ethnopharmacological relevance
Laurus nobilis L. (Lauraceae) has been used for folk medicines in the Mediterranean area and Europe to treat various disorders including skin inflammation (dermatitis) and asthma.Aim of the study
Our aim was to investigate the scientific evaluation of the compounds from Laurus nobilis L. on immuniglobulin E (IgE)-mediated type I hypersensitivity responses in vitro such as atopic dermatitis and asthma.Methods and materials
Seven compounds were isolated and examined for the mast cell stabilizing effect on IgE-sensitized RBL-2H3 mast cells by measuring the β-hexosaminidase activity. In addition, the effects on interleukin (IL)-4 production and IL-5-dependent Y16 early B cell proliferation were investigated as well as their cytotoxic effects on RBL-2H3 cells.Results
Among the seven isolated compounds, magnolialide attenuated the release of β-hexosaminidase from RBL-2H3 cells with an IC50 value of 20.2 μM, while the other compounds revealed no significant effects at concentrations tested. Furthermore, magnolialide significantly inhibited the IL-4 release with an IC50 value of 18.1 μM and IL-4 mRNA expression with an IC50 value of 15.7 μM in IgE-sensitized RBL-2H3 cells. In addition, the inhibition of IL-5-dependent proliferation of early B cells (Y16 cells) by magnolialide was demonstrated with an IC50 value of 18.4 μM.Conclusion
These results suggest that the magnolialide might be a candidate for the treatment of IgE-mediated hypersensitivity responses such as atopic dermatitis and asthma by inhibiting mast cell degranulation, the IL-4 production, and IL-5-dependent early B cell proliferation, key factors in the development and amplification of type I hypersensitivity reactions. 相似文献18.
Ivan L. Lawag Alicia M. Aguinaldo Suad Naheed Mohammad Mosihuzzaman 《Journal of ethnopharmacology》2012
Ethnopharmacological relevance
Antidesma bunius Spreng. (Phyllantaceae), Averrhoa bilimbi L. (Oxalidaceae), Biophytum sensitivum (L.) DC. (Oxalidaceae), Ceriops tagal (Perr.) C.B. Rob. (Rhizophoraceae), Kyllinga monocephala Rottb. (Cyperaceae), and Rhizophora mucronata Lam. (Rhizophoraceae) are used as remedies to control diabetes. In the present study, these plants were screened for their potential α-glucosidase inhibitory activity.Materials and methods
The 80% aqueous ethanolic extracts were screened for their α-glucosidase enzyme inhibitory activity using yeast alpha glucosidase enzyme.Results
Except for A. bilimbi with IC50 at 519.86±3.07, all manifested a significant enzyme inhibitory activity. R. mucronata manifested the highest activity with IC50 at 0.08±1.82 μg mL−1, followed by C. tagal with IC50 at 0.85±1.46 μg mL−1 and B. sensitivum with IC50 at 2.24±1.58 μg mL−1.Conclusion
This is the first report on the α-glucosidase inhibitory effect of the six Philippine plants; thus, partly defining the mechanism on why these medicinal plants possess antidiabetic properties. 相似文献19.
Mohammad Ebrahim Rezvani Ali RoohbakhshMohammad Allahtavakoli Ali Shamsizadeh 《Journal of ethnopharmacology》2010
Ethnopharmacological relevance
Valeriana officinalis L. (valerian) root extract has been used as an antiepileptic herbal medicine in Iran.Aim of this study
In the present study the effect of valerian extracts on an experimental model of temporal lobe epilepsy (TLE) was evaluated. Moreover, the involvement of adenosine system in the actions of aqueous extract of valerian was evaluated.Materials and methods
Bipolar stimulating and monopolar recording electrodes were implanted stereotaxically in the right basolateral amygdala of male Sprague–Dawley rats. After kindling, the effect of aqueous (200, 500 and 800 mg/kg; intraperitoneal) and petroleum ether (PE; 50 and 100 mg/kg; intraperitoneal) extracts of valerian and CPT (selective A1 receptor antagonist; 10 and 20 μM; intracerebroventricular) on afterdischarge duration (ADD), duration of stage 5 seizure (S5D) and latency to the onset of bilateral forelimb clonuses (S4L) were measured. The effect of CPT (10 μM) on the response of aqueous extract of valerian (500 mg/kg) was also determined.Results
The results showed that aqueous extract of valerian had anticonvulsant effect. However, PE extract and CPT (20 μM) had proconvulsant effect. Administration of CPT (10 μM) before the administration of aqueous extract decreased the anticonvulsant effect of valerian.Conclusions
The results showed significant anticonvulsant effect for aqueous but not PE extract of valerian. Moreover, CPT as a selective adenosine A1 receptor antagonist decreased the anticonvulsant effect of valerian aqueous extract. Therefore, we concluded that part of anticonvulsant effect of valerian probably is mediated through activation of adenosine system. 相似文献20.