Traditional Chinese Medicine Compound Mianbu Mix－fang Is Effective in Treating Immunological Infertility：a Female Mice

Objective To study the therapeutic effectivess of Traditional Chinese Medicinecompound mixture Mianbu Fang (Immunological infertility therapy) on immunologi-cal infertility caused by antisperm antibody (AsAb) in female mice.Materials & Methods Forty-two female Kunming mice were evenly divided into 7groups by weight. Group A was control group; Group B was model of infertility.Group C, D and E were fed with normal, half and double dosage of Mianbu Ⅰ respec-tively. Group F and G were fed with Mianbu Ⅱ and prednisone Acetates respectively.Animal model of immunological infertility were set up by injecting mice sperm to theother 36 Kunming female mice except Group A. The AsAb levels in serum, cervicalmucus were measured, the histological and immunohistochemistry changes in ovary andendometrium were observed, and the pregnancy indexes were compared in differentgroups.Results Compared with the infertility model group, the AsAb level in serum andcervical mucus in treatment group was lower. Less immune compounds in ovary andendometrium and atretic follicle of ovary was found in treatment group than in modeland control group. The immune compounds in ovary and endometrium were less in thetreatment group than that in the model and control group.Conclusion By regulating immunological system, Traditional Chinese Medicinecompound mixfang Mianbu Fang lowers AsAb in the circulation system and specialorgans, eliminates immunological compound, repairs tissue impairment and increases pregnancy of female mice.     

Histology and Immunohistochemistry Study on Mianbu Fang Healing Immunological Infertility of Male Mice

Recently,immunological infertility due to As Ab attracted greatinterests amongdomestic and oversea scholars. Traditional Chinese Medicine ( TCM) have been usedin curing the immunological infertility by domestic scholars,butmostly in clinic[1 - 2 ] .Meanwhile,experimental studies on immunological infertility were carried out re-cently[3 ,4] .LIANG Guo-zheng etal,[3 ] used theindex of serum As Ab of rat,LAIAn-ni[4] used the index of serum As Ab and cytotoxic antibody to sperm in female m…     

Histology and Immunohistochemistry Study on Mianbu Fang Healing Immunological Infertility of Male Mice

Objective To observe the histological and immunohistochemical changes caused by of Traditional Chinese Medicine--Mianbu Fang (Immunological Infertility Healing Mixture) in testis and epididymus of male mice with immunological infertility induced by antisperm antibody (AsAb ). Materials & Methods Models of mice with immunological infertility were used by in jection of mice sperm. These mice were treated with Mianbu Ⅰ , Mianbu Ⅱ or Pred nisone Acetates. The characteristics on histology and immunohistochemistry of mice were analyzed. Results High level of AsAb was detected in serum and seminal vesicle plasma of model mice after treatment. Immune compounds mostly deposited in limiting membrane of seminiferous tubules, spermatogonium and epithelia of epididymis ducts. The num ber of spermatogenic cells per convoluted seminiferous tubule decreased. However, the Chinese medicine could significantly reduce the level of As Ab, or even eliminate the an tibodies and clear out the immune compounds and increase the number of spermatogenic cells of seminiferous tubules. Conclusion Chinese Medicine Mianbu Ⅰ and Ⅱ can regulate, absorb and clear out cir culation and partial AsAb, as well as immunological compound. Hence, they can in crease the number of spermatogenic cells of seminiferous tubules and increase the preg nancy of mice.     

中医药治疗无排卵性不孕症临床疗效Meta分析

目的:运用循证医学的方法对中医药治疗无排卵性不孕症的疗效进行系统评价。方法:检索并查阅1999年1月——2009年1月期间以无排卵性不孕患者为研究对象,设有中医治疗组的临床研究文献,采用Cochrane系统评价方法,选取符合标准的临床研究文献资料,进行Meta统计分析。结果:入选文献共4篇,总病例数448例。Meta分析结果显示中医药能显著治疗无排卵性不孕症,提高妊娠率;倒漏斗图显示不对称,可能与发表偏倚及临床试验设计有关。结论:中医药治疗无排卵性不孕症是一种有效的方法,但临床随机对照试验的方法学质量较低,存在潜在的发表偏倚。     

免疫性不孕的中医药治疗

目的：探讨中医药对免疫性不孕的认识。方法：通过对临床报道的总结，阐明中医理论对免疫性不孕病因病机的认识及运用中医药对免疫性不孕抗精子抗体(AsAb)阳性患者的分型论治。结果：该病发病与肾虚、肝郁、血瘀、湿热下注有关。治疗多从整体调理，辨证论治，能使患者AsAb转阴并妊娠。结论：中医药治疗本病近期远期疗效肯定，有独到之处。     

排卵障碍性不孕症的中医药治疗进展

中医药治疗排卵障碍性不孕症疗效确切,无明显不良反应。随着越来越多的中医学者在本病的治疗方面进行不断的研究,中医药对本病的治疗已显示出越来越大的优势。本文从辨证论治、中药人工周期疗法、针药结合、中西医结合疗法几个方面阐述了近年来中医药治疗排卵障碍性不孕症的概况及进展。     

Chinese Medicine as Complementary Therapy for Female Infertility

Chinese medicine (CM) has been used in clinical treatment for thousands of years in China, Japan, Korea, and other countries. CM is at present attracting many attentions around the world for reproductive health care and disease prevention, including treatment of female infertility. This review focuses on the CM treatment for female infertility patients, and supplies a summary on the efficacy, safety, and mechanism of some Chinese herbal medicines, herbal medicine-derived active compounds, and acupuncture. A large number of researches have reported that CM could alleviate or even cure female infertility by regulating hormone, improving reproductive outcome of in vivo fertilization, affecting embryonic implantation, curing polycystic ovarian syndrome, endometriosis, pelvic inflammatory disease, relieving mental stress, and regulating immune system. Meanwhile, a few studies claimed that there was little adverse reaction of CM in randomized controlled trials. However, up to present there is a lack of adequate evidences with molecular mechanistic researches and randomized controlled trials to prove the CM as an effective and safe treatment for infertility. Thus, utility of CM as a complementary medicine will be a feasible method to improve the outcome of female infertility treatment.     

不孕不育患者的免疫因素分析

目的 :了解本地区不孕不育患者免疫不孕的因素。方法 :对 1 1 1 5例不孕不育患者用酶联免疫吸附法(ELISA)分别联合测定血清中的AsAb、EmAb、AoAb、HCGAb、ATAb、ACA。结果 :1 74例男性的AsAb阳性率为4 6 .5 5 %。 94 1例女性中 ,六种抗体的阳性率分别为 1 9.34%、1 4 .96 %、1 1 .0 5 %、1 0 .31 %、7.2 6 %和 1 5 .30 %。原发性不孕的AsAb阳性率为 2 2 .33% ;继发性不孕的AsAb阳性率为 1 1 .32 % ,两者比较 χ2 =1 4 .4 7,P <0 .0 1。结论 :在不孕不育临床中最常见的是由于精子免疫引致的男、女性免疫不育 ,尤以原发性不孕为甚 ,不孕不育患者进行免疫因素的诊断和治疗是必要的     

治疗艾滋病的中西药物比较

近年来,治疗艾滋病的药物较多,经临床验证,西药均有一定的副作用.中草药研究对治疗艾滋病带来了新的契机,临床治疗显示,中草药用药安全,有抗病毒协同作用及长期使用毒副反应低等优点.     

免疫性不育阴虚证小鼠模型造模方法研究

目的:建立免疫性不育阴虚证小鼠模型。方法:昆明小鼠90只,雌雄各半,于第1、15、29、36、43、50天先后注射精子-完全佐剂,精子-不完全佐剂。第1、2次注射精子密度130×106/mL,以后每次注射精子密度为50×106/mL,注射部位分别为前后足掌、背部正中、背部多点、左右腹股沟。第1、2次注射0.3mL,以后每次注射0.2mL。第44~50天给小鼠按照320mg/(kg.d)灌胃甲状腺片混悬液,连续灌胃7天。结果:有84只昆明小鼠出现明显的怕热、多汗、神经过敏、急躁、震颤、皮毛光泽度差、饮水量减少等阴虚的现象。而其中有79个样本经检测AsAb吸光度/正常对照组≥2.1,即为AsAb(+)。说明在90只昆明小鼠中,有79只进行免疫性不育阴虚证造模成功。结论:免疫性不育阴虚证小鼠模型的成功制备对临床研究和中医药治疗免疫性不育症疾病等起到了重要的作用。     

中医药治疗围绝经期综合征临床研究

随着生活水平的提高,女性围绝经期保健越来越受到重视,中医药对围绝经期综合征治疗的研究也逐渐加深,文章收集了近5年来有关围绝经期综合征的中医药临床研究相关文献,分别从中医药治疗围绝经期综合征的临床疗效、中医情志干预疗效、中医药治疗对患者生活质量及血清激素影响等方面的研究作了综述,并对将来的研究作了展望。     

中药在溃疡性结肠炎治疗中的疗效优势

从提高疗效、缩短症状改善时间、降低复发率及治疗伴随症状四方面阐述中药对溃疡性结肠炎的影响,说明中药在溃疡性结肠炎治疗中具有重要作用.     

2004～2010年中成药及中药的应用分析

目的：了解我院门诊使用中成药及中药的情况及发展趋势。方法：收集我院2004～2010年间的门诊就诊人数、使用中成药及中药的人数、中成药及中药的销售总金额,并计算出每年使用中成药及中药人数与门诊就诊人数比、中成药及中药处方的平均费用金额,并进行统计分析。结果：2004～2010年中成药的使用呈上升趋势,而中药的使用则相对呈下降趋势;2007～2009年中成药处方平均费用金额显著增长,2009—2010年中药处方平均费用金额显著增长。结论：我院门诊中成药使用广泛,具有独特优势和巨大潜力,中药的使用则需进一步加强。     

中药安迪粉针剂对小鼠巨噬细胞cAMP含量的影响

目的：观察中药安迪粉针剂（Andi）对小鼠巨噬细胞（MΦ）cAMP含量的影响。方法：采用细胞培养和竞争性蛋白结合分析法测定小鼠腹腔MΦ中cAMP含量。结果：（1）cAMP水平的变化具有规律性：VB在3min以内可引起小鼠腹腔MΦ中cAMP浓度快速升高，6min时达到峰值，之后缓慢下降，至30min时基本恢复原来水平，RG和Andi则是3min开始升高，到15min达峰值，然后逐渐下降，180min时已基本恢复至原来水平，其升高程度显高于VB（P＜0．05）；（2）Andi能明显引起小鼠腹腔MΦcAMP升高，并在（6－48）h.L^-1剂量范围时呈量效依赖关系。结论：Andi引起小鼠MΦcAMP浓度升高，可能是Andi提高小鼠免疫功能的机制之一。     

输卵管阻塞性不孕中医药治疗研究进展

输卵管阻塞是引起女性不孕的重要原因,且发病率呈上升趋势。随着腹腔镜手术、介入手术的应用,输卵管复通率提高,但仍因术后存在输卵管蠕动、捡卵、纤毛运动功能及短期内不受孕容易再次黏连、阻塞等问题,所以妊娠率并不高;临床实践与研究表明,中医药、中西医结合治疗该病具有提高妊娠率等较好的疗效,值得临床推广应用。现将近年来采用中医药治疗输卵管阻塞性不孕症的研究进展作一综述,以利于本病更好的治疗。     

输卵管阻塞陛不孕中医药治疗研究进展

输卵管阻塞是引起女性不孕的重要原因,且发病率呈上升趋势。随着腹腔镜手术、介入手术的应用,输卵管复通率提高,但仍因术后存在输卵管蠕动、捡卵、纤毛运动功能及短期内不受孕容易再次黏连、阻塞等问题,所以妊娠率并不高;临床实践与研究表明,中医药、中西医结合治疗该病具有提高妊娠率等较好的疗效,值得临床推广应用。现将近年来采用中医药治疗输卵管阻塞性不孕症的研究进展作一综述,以利于本病更好的治疗。     

中西医结合治疗不孕症疗效观察

目的 :探析中西医结合疗法治疗不孕症的临床疗效。方法 :50例卵巢性不孕的患者共155个周期接受了中西医结合治疗,月经周期5~9 d口服克罗米芬50 mg/d。同时服用自拟的促卵泡汤及促排卵汤,用B超测卵泡,当优势卵泡平均直径≥18 mm时,肌内注射HCG 10000 U促排卵。结果 :排卵率79.4%,妊娠率为76%,未破裂卵泡黄素化发生率9.0%。结论 :中西医结合疗法治疗不孕症有良好疗效。     

秋冬季咳嗽中医治疗体会

目的：探讨自拟咳嗽方治疗咳嗽的临床疗效。方法：选择秋冬季接诊的昆明地区80例咳嗽患者进行观察。结果：临床控制12例（15．00％）,显效40例（50．00％）,有效25例（31．35％）,无效3例（3．75％）,总有效率96．25％。结论：v自拟咳嗽方治疗昆明地区秋冬季咳嗽患者有效。     

中药联合枸橼酸氯米芬胶囊治疗排卵障碍性不孕症的临床观察

目的：探讨中药联合枸橼酸氯米芬胶囊治疗排卵障碍性不孕症的疗效。方法方便收集2014年5月—2015年10月在该院妇科门诊就诊的排卵障碍性不孕症患者100例,根据随机数字表法将上述患者分为观察组和对照组,每组50例。对照组单纯采用枸橼酸氯米芬胶囊治疗,观察组在对照组基础上加中医药汤剂治疗,比较两组治疗前、排卵期的卵泡内径及子宫内膜厚度变化及排卵率、妊娠率和疗效的差异。结果观察组卵泡期的卵泡内径为(23.9±4.3)mm,子宫内膜厚度为(9.7±3.2)mm,比对照组的(22.5±4.1)mm、(7.1±3.2)mm明显改善(P<0.05)。观察组妊娠率、总有效率分别为66.0%、82.0%,明显高于对照组的32.0%、72.0%(P<0.05)。结论对于排卵障碍性不孕症,中药汤剂配合枸橼酸氯米芬胶囊有调经、促排卵、促进子宫内膜增厚,利于胚胎着床,提高妊娠率,是排卵障碍性不孕症较好的一种高效安全的疗效。     

中西医结合治疗带状疱疹体会

目的：介绍中西医结合治疗组及单纯西医组治疗带状疱疹的体会。方法：西治组以抗病毒、营养神经、对症、支持为主;中西医结合治疗组在西治组基础上加用贞芪扶正胶囊和愈疱汤口服。结果：中西医结合治疗组愈显率91．67％,总有效率97．22％,西治组愈显率67．65％,总有效率91．18％．经统计学处理,两组愈显率差异有显著性（P〈0．01）,中西医结合治疗组疗效优于西治组。结论：中西医结合治疗组效果优于单纯西医组。