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凋亡抑制蛋白Livin在肿瘤治疗中的新进展 总被引:1,自引:0,他引:1
Livin是凋亡抑制蛋白的一个新成员,可以编码抑制凋亡的负性调节蛋白.Livin在大多数肿瘤中高表达,体内外研究发现其表达降低可以增加肿瘤细胞凋亡,降低肿瘤细胞的生长潜能,增加肿瘤细胞对化疗的敏感性.因此,Livin可以作为肿瘤治疗的新的靶点. 相似文献
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凋亡抑制蛋白Livin:抗癌新靶点 总被引:2,自引:0,他引:2
Livin,又称ML-IAP或KIAP是与肿瘤发生发展密切相关的凋亡抑制蛋白家族( inhibitor of apoposis proteins, IAPs)中的新成员,能够与内源IAP抑制剂SMAC 和 caspase-3,caspase-7,caspase-9结合,发挥抗细胞凋亡的作用.它特异性高表达于肿瘤组织而使肿瘤组织对放化疗作用变得耐受.近来,体内外研究表明下调或抑制Livin基因表达增加了肿瘤细胞凋亡率,抑制肿瘤细胞的增殖和集落形成能力,提高肿瘤细胞对化疗药物和放疗的敏感性.Livin 将成为肿瘤治疗的潜在靶点. 相似文献
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Livin是凋亡抑制蛋白( inhibitor of apoptosis proteins,IAPs)家族的最新成员,其在正常成人的大多数终末分化组织中不表达或低表达,而在许多恶性肿瘤组织中明显过表达。Livin及其相关基因的检测,对肿瘤的早期诊断和预后判断具有参考价值;靶向抑制肿瘤细胞中Livin的表达,能为肿瘤的治疗开辟新途径。本文就Livin基因与泌尿系肿瘤的国内外研究进展及其与肿瘤靶向治疗的研究近况予以综述。 相似文献
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Livin与肿瘤研究新进展 总被引:13,自引:2,他引:11
Livin是凋亡抑制蛋白家族的新成员,具有凋亡抑制蛋白家族的特征性结构——BIR结构域。这一结构域介导了Livin与Caspase-3、7、9的直接结合,从而阻断caspase凋亡作用的发挥,达到抑制细胞凋亡的作用。Livin基因定位于染色体20q13,编码Livinα和β两种蛋白。亚细胞定位显示,在细胞质和细胞核均有表达,羧基末端的RING结构域介导了Livin的亚细胞定位。目前的研究表明,Livin可表达于消化系统的胃癌、泌尿系统的膀胱癌、呼吸系统肺癌和血液系统的淋巴细胞性白血病等多个系统的肿瘤中,也存在于乳腺癌及黑色素瘤中,在肿瘤的细胞系中也有较广泛的表达,但在各系统的正常组织中却很少表达。研究还表明,在药物诱导凋亡时,Livin的表达有明显升高。用RNA干扰技术对Livin基因的表达进行干预后,细胞对诱导凋亡药物和放射线的敏感性增加。根据Livin设计的抗原肽可以特异性诱导肺癌患者杀伤性T细胞的活化。目前的研究已表明,Livin在肿瘤的发展和耐药中都有重要作用。Livin特异表达于肿瘤组织的特性有可能为肿瘤的早期诊断、基因治疗和细胞治疗提供新靶点。 相似文献
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Livin基因在恶性肿瘤诊断和治疗中的研究进展 总被引:2,自引:0,他引:2
Livin作为凋亡抑制蛋白家族(inhibitor of apoptosis proteins,IAPs)的成员之一,表达于多种恶性肿瘤细胞中,而且其过表达可增加恶性肿瘤细胞的抗凋亡作用及对放疗化疗的耐受性。 相似文献
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目的:构建Livin靶向小干扰RNA(siRNA)重组表达载体,观察其诱导大肠癌细胞的凋亡效应.方法:构建Livin靶向siRNA重组表达栽体并转染结肠癌细胞,RT-PCR和蛋白质印迹法检测Livin的表达,MTT法检测siRNA对细胞增殖的抑制作用,流式细胞仪检测细胞的凋亡效应.结果:经酶切鉴定和测序结果证实,Livin靶向sirNA重组表达载体构建成功,它对大肠癌细胞Livin mRNA和蛋白的表达抑制率分别为30.18%和28.88%,P<0.05,肿瘤细胞的生长受到明显抑制,细胞凋亡率为(13.36±1.45)%,P<0.05.结论:Livin靶向siR-NA重组表达载体构建成功,能有效抑制Livin基因表达并能显著诱导肿瘤细胞凋亡. 相似文献
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Liu Chuan Wu Xiaohou Luo Chunli Hu Zili Yin Zhikang He Yunfeng Du Hu Zhang Weili Jiang Qing Lin Yanjun 《Journal of experimental & clinical cancer research : CR》2010,29(1):63
Background and Aim
in recent years, Livin, a new member of IAPs family, is found to be a key molecule in cancers. Researchers consider Livin may become a new target for tumor therapy; however, the role of it in bladder cancer is still unclear. The purpose of this article is to investigate Antisense Oligonucleotide (ASODN) of Livin on treating bladder cancer cell and underlying mechanisms.Methods
Phosphorathioate modifying was used to synthesize antisense oligonucleotides targeting Livin, followed by transfection into human bladder cancer cell 5637. After transfection, Livin mRNA and protein level, cell proliferation and apoptosis changes, caspase3 level and its effect on human bladder cancer transplantable tumor in nude mice were measured.Result
results showed Livin ASODN effectively inhibited Livin expression and tumor cell proliferation, and these effects probably through enhanced caspase3 activity and apoptosis of tumor cells. In nude mice transplantable tumor model, Livin expressions were inhibited meanwhile caspase3 expression was increased. Tumor growth slowed down and apoptosis was enhanced.Conclusion
Our data suggest that Livin plays an important role in inhibiting apoptosis of bladder cancer cells. Livin ASODN may promote cell apoptosis, inhibit bladder cancer growth, and become one of the methods of gene therapy for bladder cancer. 相似文献12.
凋亡蛋白抑制因子(inhibitor of apoptosisprotein,IAP)是一类抑制凋亡的调节分子,其成员livin是新发现的凋亡抑制基因,存在同基因不同剪切的两个异构体:Livinα和Livinβ;livin在多数肿瘤组织中高表达;其作用机制主要通过:1)抑制半胱天冬酶3/7/9(caspase3/7/9)的活性;2)激活TAK1/JNK1信号转导途径,同过非C途径抑制TNF及ICE诱导的细胞凋亡,来阻断细胞凋亡过程。livin与肿瘤发生有密切关系,并可能作为诱导肿瘤凋亡治疗的新靶点 相似文献
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凋亡蛋白抑制因子(inhibitor of apoptosis protein,IAP)是一类抑制凋亡的调节分子,其成员livin是新发现的凋亡抑制基因,存在同基因不同剪切的两个异构体:Livina和Livinβ;livin在多数肿瘤组织中高表达;其作用机制主要通过:1)抑制半胱天冬酶3/7/9(caspase3/7/9)的活性;2)激活TAK1/JNK1信号转导途径,同过非C途径抑制TNF及ICE诱导的细胞凋亡,来阻断细胞凋亡过程。livin与肿瘤发生有密切关系,并可能作为诱导肿瘤凋亡治疗的新靶点 相似文献
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目的 探讨Livin表达影响水疱口炎病毒(VSV)对肺癌A549细胞的促凋亡效应。方法 利用RNA干扰技术下调Livin在肿瘤细胞中的表达,通过Tunel染色观察VSV对肺癌A549细胞的凋亡效应,Western blot法检测Livin蛋白的表达,荧光定量法检测Caspase-3的活性。结果 VSV(感染复数= 0.1 pfu/细胞)对A549细胞有促凋亡作用;抑制Livin的表达增强了VSV对A549细胞的促凋亡作用,增强了Caspase-3的活性。结论 Livin表达沉默后可能通过影响Caspase-3活性而增强了VSV对A549细胞的促凋亡效应。 相似文献
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Lei Yi Fengjin Guo Anmin Chen 《中德临床肿瘤学杂志》2007,6(6):587-590
Objective: To investigate the inhibitory effects of LMWH suppressing the expression of Livin and inducing the apoptosis of the osteosarcoma cells. Methods: Osteosarcoma cells line MG-63 was cultured in vitro. MTT assay and flow cytometry were used to study the effect of LMWH with different concentration suppressed the prolifetation and induced apoptosis in osteosarcoma cells line MG-63. The expression of Livin of osteosarcoma cells line MG-63 was analysed by the immunohistochemistrical method and PT-PCR. Results: Low molecular weight heparin could inhibit the growth of osteosarcoma cell line MG-63. With the LMWH's increasing, the apoptosis rate was increased significantly. Immunohistochemistrical method and PT-PCR showed that the expression of Livin of osteosarcoma cells line MG-63 declined obviously than that before medication. Conclusion: LMWH has very strong anti-tumor effect in vitro. The possible mechanisms of LMWH anti-tumor effect are associate with the effect of suppressing the expression of Livin and inducing cell apoptosis. 相似文献
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Livin, a novel member of the human inhibitors of apoptosis protein family, plays an important role in tumor progression and
occurrence by inhibiting cell apoptosis. It is selectively expressed in the most common human neoplasms and appears to be
involved in tumor cell resistance to chemotherapeutic agents. The present study was designed to investigate the potential
of using RNA interference (RNAi) technique to downregulate Livin expression, and the subsequent effect on human glioma cells.
The results showed that knockdown of Livin expression by short interfering RNA (siRNA) significantly inhibited glioma cell
proliferation and increased cell apoptosis through cell arrest in the G1/G0 phase of cell cycle in vitro. Furthermore, Livin siRNA significantly suppressed tumor growth in nude mice. Together, these
findings suggest that RNAi-mediated downregulation of Livin expression could lead to potent antitumor activity in glioma cells
and might serve as a novel therapeutic strategy in clinic. 相似文献