复印作业对人体氧化和过氧化的影响

目的探讨复印作业对人体氧化和过氧化的影响。方法检测１０４例复印作业者和１００例健康成人血浆ＶｉｔＣ、ＶｉｔＥ、β－胡萝卜素（β－ＣＡＲ）、过氧化脂质（ＬＰＯ）含量及红细胞超氧化物歧化酶（ＳＯＤ）、过氧化氢酶（ＣＡＴ）、谷胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ）活力和ＬＰＯ含量。结果与健康成人组比较，复印作业组的血浆ＶｉｔＣ、ＶｉｔＥ、β－ＣＡＲ和红细胞ＳＯＤ、ＣＡＴ、ＧＳＨ－Ｐｘ平均值皆显著降低（Ｐ＜０．０１），而血浆及红细胞ＬＰＯ平均值明显升高（Ｐ＜０．０１），３种不同机型复印作业组各指标平均值间皆差异无显著性（Ｐ＞０．０５），配备通风装置组与未配备通风装置组各指标平均值间差异皆有显著性（Ｐ＜０．０１），复印作业工龄与各指标间均呈一定程度的直线相关（Ｐ＜０．０２～０．０００１）。结论复印从业人员体内氧化和过氧化反应加剧     

矽肺与NO、氧化及脂质过氧化关系的探讨

目的探讨矽肺与ＮＯ及氧化和脂质过氧化的关系。方法检测７３例矽肺患者和６０例健康对照者的血浆ＮＯ、ＶｉｔＣ、ＶｉｔＥ、β胡萝卜素（βＣＡＲ）、过氧化脂质（ＬＰＯ）含量及红细胞超氧化物歧化酶（ＳＯＤ）、过氧化氢酶（ＣＡＴ）、谷胱甘肽过氧化物酶（ＧＳＨＰｘ）活力和ＬＰＯ含量。结果与对照组比较,矽肺组的血浆ＶｉｔＣ、ＶｉｔＥ、βＣＡＲ含量及红细胞ＳＯＤ、ＣＡＴ、ＧＳＨＰｘ活力显著降低,血浆ＮＯ、ＬＰＯ含量及红细胞ＬＰＯ含量显著升高（Ｐ＜０．０５或Ｐ＜０．０１）;各检测值与病程均有相关;血浆ＮＯ、ＶｉｔＥ含量及红细胞ＳＯＤ活力、ＬＰＯ含量与病程相关最密切。结论矽肺患者体内的ＮＯ代谢异常,氧化和脂质过氧化反应病理性加剧。     

硒和维生素E缺乏对大鼠胰岛功能的影响

以低硒酵母（Ｔｏｒｕｌａｙｅａｓｔ）及低硒玉米淀粉为主要成分配成低硒、低ＶＥ及硒和ＶＥ联合缺乏饲料喂饲大鼠，引起血清胰岛素、Ｃ肽水平显著下降（Ｐ＜０．０１），胰岛内胰岛素与Ｃ肽分泌贮备明显减少（Ｐ＜０．０１），并伴有血及胰腺组织ＧＳＨ－Ｐｘ活性显著降低（Ｐ＜０．０１），ＬＰＯ含量明显上升（Ｐ＜０．０５～０．０１），在饲料中补０．２ｍｇ／ｋｇ硒或５００ｍｇ／ｋｇＶＥ，可明显提高血清胰岛素、Ｃ肽水平（Ｐ＜０．０１），增加胰岛素与Ｃ肽分泌贮备（Ｐ＜０．０１），同时伴有血及胰腺ＧＳＨ－Ｐｘ活性水平升高与ＬＰＯ含量降低（Ｐ＜０．０５～０．０１），提示硒和ＶＥ缺乏是胰岛损害的重要病因之一。     

干扰素治疗丙型肝炎疗效相关因素分析

目的：探讨预测干扰素治疗慢性丙型肝炎（ＣＨＣ）疗效的临床上适用、简便、易行的指标。方法：对采用干扰素（ＩＦＮα－１ｂ）治疗３２例ＣＨＣ临床资料进行观察,包括治疗前后肝功能（ＡＬＴ、ＳＢ、ＧＧＴ）、抗－ＨＣＶＯＤ水平、ＨＣＶ基因型、ＨＣＶＲＮＡ、ＳＩＬ－２Ｒ、β２－ＭＧ等,治疗结束时有效者为肝功能复常同时ＨＣＶＲＮＡ阴转,其余反应模式均为无效者。结果：ＨＣＶ基因型为Ⅲ型患者疗效（５／１０）明显高于Ⅱ型（２／１９）（Ｐ＜０．０５）,有效者治疗前血清抗—ＨＣＶＯＤ明显低于无效者（Ｐ＜０．０５）,治疗后有效者β２－ＭＧ升高水平（３．４５±１．０８）ｍｇ／Ｌ明显高于无效者（１．３３±０．９５）ｍｇ／Ｌ（Ｐ＜０．０５）,而两者治疗前ＡＬＴ、ＳＢ、ＧＧＴ、ＩｇＭ、β２－ＭＧ患者的年龄无明显差别（Ｐ＞０．０５）。结论：感染ＨＣＶ基因型,治疗前抗—ＨＣＶＯＤ水平及治疗后β２－ＭＧ变化对预测ＩＦＮ治疗ＣＨＣ疗效有一定参考价值。     

尘肺患者血清β－胡萝卜素与抗氧化功能的研究

本研究测定了尘肺病人、慢性支气管炎（慢支）病人及健康成人的血清β－胡萝卜素（ＢＣ）和ＶｉｔＡ含量以及血清脂质过氧化酶（ＬＰＯ）含量，超氧化物歧化酶（ＳＯＤ）和谷胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ活性。结果发现，尘肺组血清ＢＣ含量明显低于健康组和慢支组（Ｐ＜０．０１）。而血清ＶｉｔＡ含量３个组相近（Ｐ＞０．０５）。与健康组和慢支组比较，尘肺组血ＬＰＯ含量明显升高，ＳＯＤ和ＧＳＨ－Ｐｘ活性则明显降低（Ｐ均＜０．０ｌ）。上述结果提示，尘肺病人抗氧化功能明显减弱可能与血清ＢＣ含量较低有关。     

口腔扁平苔藓患者与自身免疫功能及微量元素关系研究

对口腔扁平苔藓（ＯＬＰ）患者与自身免疫功能及微量元素的关系,检测了微量元素与免疫功能,结果表明：①ＯＬＰ患者辅助性Ｔ细胞（Ｔｈ细胞）明显低于正常人（Ｐ＜０．０１）,抑制性Ｔ细胞（ＴＳ细胞）明显高于正常人（Ｐ＜０．０１）,表明ＯＬＰ患者细胞免疫水平处于抑制状态。②血清ＩｇＧ,ＩｓＡ,ＩｇＭ含量均高于正常人。③ＯＬＰ患者血清锌（Ｚｎ）明显低于正常人（Ｐ＜０．０５）。提示：ＯＬＰ发病机理可能与自身免疫和Ｚｎ水平有密切关系     

一氧化氮等自由基对矽肺患者损伤效应的研究

检测７３例矽肺患者和６０例健康对照者血浆一氧化氮（Ｐ－ＮＯ）、维生素Ｃ（Ｐ－ＶＣ）、维生素Ｅ（Ｐ－ＶＥ）、β－胡萝卜素（Ｐ－β－ＣＡＲ）、过氧化脂质（Ｐ－ＬＰＯ）及红细胞超氧化物歧化酶（Ｅ－ＳＯＤ）、过氧化氢酶（Ｅ－ＣＡＴ）、谷胱甘肽过氧化物酶（Ｅ－ＧＳＨ－ＰＸ）和过氧化脂质（Ｅ－ＬＰＯ）值的结果表明,与对照组比较,患者组Ｐ－ＶＣ、Ｐ－ＶＥ、Ｐ－β－ＣＡＲ、Ｅ－ＳＯＤ、Ｅ－ＣＡＴ、Ｅ－ＧＳＨ－ＰＸ平均值均显著降低（Ｐ＜０．００１）,Ｐ－ＮＯ、Ｐ－ＬＰＯ、Ｅ－ＬＰＯ平均值均显著升高（Ｐ＜０．００１）;上述检测值与患者病程、病情、肺功能状态均有相关;逐步回归表明,患者病程与Ｐ－ＮＯ、Ｐ－ＶＥ、Ｅ－ＳＯＤ、Ｅ－ＬＰＯ值相关最为密切;提示矽肺患者体内一氧化氮和氧自由基反应病理性加剧,氧化抗氧化平衡严重失调     

尘肺患者血清β—胡萝卜素与抗氧化功能的研究

本研究测定了尘肺病人，慢性支气管炎病人及健康成人的血清β－胡萝卜素和ＶｉｔＡ含量以及血甭脂质过氧化酶含量，超氧化物歧化酶和谷胱苷肽过氧化物酶活性。结果发现，尘肺组血清ＢＣ含量明显低于健康组和慢支组（Ｐ＜０．０１）。而血清ＶｉｔＡ含量３个组相近（Ｐ＞０．０５）。与健康组和慢支组比较，尘肺组血ＬＰＯ含量明显升高，ＳＯＤ和ＧＳＨ－Ｐｘ活性则明显降低（Ｐ均＜０．０１）。上述结果提示，尘肺病人抗氧化功能明显     

鱼油对感染大鼠肠粘膜的保护作用

目的：探讨膳食鱼油对感染机体小肠粘膜的保护作用。方法：将大鼠随机分为ＮＳ＋ＣＬＰ，ＦＯ＋ＣＬＰ及ＳＨＡＭ（假手术）三组（ｎ＝１５），术前分别灌食鱼油或等渗盐水１ｍｌ×３周，于ＣＬＰ后第二天采取标本检测血清ＴＮＦ、ＩＬ┐６、ＰＧＥ２浓度及小肠谷氨酰胺（Ｇｌｎ）含量，并用ＲＴ┐ＰＣＲ方法测定小肠粘膜ＴＮＦｍＲＮＡ的含量。结果：ＮＳ＋ＣＬＰ组大鼠血清ＴＮＦ、ＩＬ┐６、ＰＧＥ２水平明显高于ＳＨＡＭ组（Ｐ＜０．０１），小肠粘膜Ｇｌｎ含量显著减少（０．２８±０．１１ｖｓ０．９９±０．２１，Ｐ＜０．０１），而ＴＮＦｍＲＮＡ表达显著升高（Ｐ＜０．０１）。与之相比，灌食ＦＯ大鼠血清ＴＮＦ、ＩＬ┐６、ＰＧＥ２水平则有不同程度的下降，而小肠粘膜Ｇｌｎ含量明显高于ＮＳ＋ＣＬＰ组，同时，小肠粘膜ＴＮＦｍＲＮＡ的表达显著降低（Ｐ＜０．０１）。结论：鱼油能通过抑制花生四烯酸代谢产物的生成，改善小肠粘膜的血循环，或降低粘膜局部细胞因子的表达，阻断细胞因子对肠粘膜结构功能及细胞代谢的不利影响     

用计算机辅助精子分析系统测定TCDD对大鼠精子运动能力的影响

利用计算机辅助的精子分析系统（ＣＡＳＡ）研究了２,３,７,８—四氯二苯－Ｐ－二口恶口英（ＴＣＤＤ）对大鼠精子运动能力的影响。给２１天龄幼鼠腹腔注射ＴＣＤＤ０．１、１．０和５．０μｇ／ｋｇ,对照组注射等量体积的溶媒。在性成熟后（９０天）,用扩散法收集附睾尾精子,测定其运行速度（ＶＣＬ、ＶＳＬ、ＶＡＰ）、运动方式（ＳＴＲ、ＬＩＮ、ＢＣＦ、ＡＬＨ、ＭＡＤ等）及活动精子的比率（Ｍｏｔ％）。结果表明,ＴＣＤＤ１．０μｇ／ｋｇ组,精子的运动速度明显低于对照组（ＶＣＬＰ＜０．０５;ＶＡＰ、ＶＳＬ、ＢＣＦＰ＜０．０１）,０．５μｇ／ｋｇ时精子的运动速度、及前向性和直线性运动性能均明显降低（Ｐ＜０．０１）,活动精子的比率也明显低于对照组（Ｐ＜０．０１）。本研究结果为ＴＣＤＤ的男性生殖毒理学研究,以及ＣＡＳＡ在男性生殖毒理学研究中的应用提供了进一步参考依据     

腹股沟斜疝修补术中蛛网膜下隙阻滞配制液应用芬太尼剂量的研究

目的研究腹股沟斜疝修补术中蛛网膜下隙阻滞（腰麻）配制液中芬太尼最佳配制剂量。方法择期行腹股沟斜疝手术患者100例,ASA分级Ⅰ～Ⅱ级。按随机数字表法分为五组,每组20例。腰麻配制液：B组：0.75％布比卡因2 ml+ 0.9％氯化钠1.0 ml;BF5组：0.75％布比卡因2ml+50 μ g/ml芬太尼0.1 ml+ 0.9％氯化钠0.9 ml;BF10组：0.75％布比卡因2ml+50 μg/ml芬太尼0.2 ml+ 0.9％氯化钠0.8 ml; BF20组：0.75％布比卡因2ml+ 50μg/ml芬太尼0.4 ml+ 0.9％氯化钠0.6 ml; BF30组：0.75％布比卡因2ml+ 50μ g/ml芬太尼0.6 ml+ 0.9％氯化钠0.4 ml。患者取左侧卧位,选L2～3间隙穿刺,各组分别推注腰麻配制液3ml。测量并记录达到最高阻滞平面时间、感觉平面退到T12平面时间、运动恢复时间、麻醉效果、镇痛维持时间、手术时间及不良反应。结果BF30组达到最高阻滞平面时间较B组、BF5组、BF10组、BF20组短(P＜0.05),而B组和BF5组较BF10组、BF20组、BF30组长(P＜0.05)。BF20组感觉平面退到T12平面时间较B组、BF5组、BF10组、BF30组长（P＜0.05）,B组和BF5组较BF10组、BF20组、BF30组短(P＜0.05)。B组和BF5组运动恢复时间和镇痛维持时间较BF10组、BF20组、BF30组短(P＜0.05)。B组患者发生瘙痒例数(0)明显少于BF5组（8例）、BF10组（3例）、BF20组（6例）、BF30组（6例）（P＜0.05）。所有患者均未发生呼吸抑制。结论在0.75％布比卡因腰麻配制液中加入10 μg芬太尼可以缩短达到最高阻滞平面时间,感觉及运动恢复时间与镇痛维持时间也相应延长,在30μg内这种效应并不存在剂量依赖性。     

Ambulatory continuous intravenous infusion of fluorouracil: a feasible palliative form of chemotherapy]

A study to evaluate the feasibility and toxicity of outpatient continuous intravenous infusion of fluorouracil (5-FU) was initiated at the department of Medical Oncology of the University Hospital of Utrecht. To this purpose a subcutaneous drug delivery system (Port-a-Cath) was implanted in 36 patients with various advanced cancers. Of these patients 83% had received prior chemotherapy (including 5-FU in 62%). Ambulatory continuous-infusion pumps were used to administer 5-FU in a dosage of 300 mg/m2/24 h. The treatment was continued until tumour progression was seen, and it was interrupted in case of toxicity grade 2 or more (WHO criteria). A Port-a-Cath was implanted 37 times in the 36 patients. The main complications of this infusion system were pneumothorax (2/37), arrhythmia (1/37), catheter sepsis (2/37) and thrombosis (2/37); they were easily managed. The toxicity and feasibility of this treatment were evaluable in 30 patients. They received a median of 44 g 5-FU (range 11-136, 5 g, mean 281 mg/m2/24 h) during a median infusion time of 12 weeks (range 4-32 w). Side effects were encountered in 70% of the patients and consisted of the hand-foot syndrome (14/30), nausea and vomiting (8/30), diarrhoea (8/30) and stomatitis (7/30). The toxicity was completely reversible after a short interruption of the chemotherapy. The treatment was tolerated well, and good palliation was attained in 22 of 30 patients. The best response was seen in patients with colon and breast cancer. We conclude that continuous infusion of 5-FU is a reliable outpatient chemotherapy even in this category of patients.     

Optimal protein intake estimated by the resistance to streptococcal infection and the nutritional indices in mice

Effects of dietary protein levels on the resistance against a bacterial infection and on the nutritional status were studied in mice to obtain basic data for the estimation of an optimal protein intake. Female DDY strain weanling mice were fed 5, 7, 10, 20, 30, or 40% casein diet. At 2 or 4 weeks on the diets, the mice were injected intraperitoneally with 5 x 10(3) or 5 x 10(4) group B streptococci/g body weight and their survival rates were observed for the following 10 days. Nutritional indices and cell numbers of thymus and spleen were also measured. The survival rate was higher in the order of 7, 10, 20, 30, 5, 40% casein diet group. Significant differences were observed between the 7% group and the 30, 5, or 40% group, and between the 10% group and the 5 or 40% group. The nutritional indices and cell numbers of the thymus and spleen were similar among the 20, 30, and 40% casein diet groups and decreased in the order of 10, 7, 5% casein diet groups. From the results, protein levels were categorized into 4 groups: severe protein deficiency with low resistance (5% casein diet), moderate protein deficiency with high resistance (7 and 10% casein diets), normal protein intake with normal resistance (20% casein diet), and high protein intake with low resistance (30 and 40% casein diets). This grouping suggests that when the relation between immunocompetence and nutritional status is considered, both high and low protein intakes are undesirable and the optimal level of dietary protein will be limited to a narrow range.     

Biochemical evidence for activation of specific monoamine pathways by ethanol

The effects of an acute intraperitoneal (IP) low (0.5 g/kg) or high (2.5 g/kg) dose of ethanol on the contents of dopamine (DA), 3,4-dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA), serotonin (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) in 7 selected CNS regions of the rat were examined after 15, 30 and 60 minutes. The IP administration of 0.5 g/kg ethanol produced blood alcohol concentrations (BACs) of 41 +/- 4, 40 +/- 4 and 15 +/- 1 mg% (N = 8 each) after 15, 30 and 60 minutes, respectively. This low dose of ethanol did not alter the levels of DA, DOPAC, HVA, 5-HT and 5-HIAA in any of the 7 CNS regions at any of the time points examined. The dose of 2.5 g/kg ethanol produced BACs of 254 +/- 26, 268 +/- 20 and 282 +/- 10 mg% (N = 8 each) after 15, 30 and 60 minutes, respectively. This high dose of ethanol did not alter the contents of DA and 5-HT in any of the regions examined at any of the times, except for a 30% increase in the content of DA in the posterior striatum after 60 minutes. The administration of 2.5 g ethanol/kg elevated the levels of DOPAC and/or HVA 25 to 70% over saline control values in the (a) nucleus accumbens (ACC) and hypothalamus (HYPO) after 15, 30 and 60 minutes, and (b) posterior striatum (PSTR), lateral septal nucleus (LSN) and frontal cortex (FCTX) after 60 minutes. The contents of DOPAC and/or HVA were not altered by the high dose of ethanol in either the thalamus or olfactory bulbs.(ABSTRACT TRUNCATED AT 250 WORDS)     

小儿斜视矫治术可弯曲喉罩下七氟烷吸入麻醉的效果

目的 评价可弯曲喉罩通气下七氟烷麻醉用于小儿斜视矫治术的效果.方法 择期行斜视矫治术患儿60例,按随机数字表法分为气管导管组和可弯曲喉罩组,每组30例.气管导管组静吸复合麻醉,气管插管,机械通气;可弯曲喉罩组七氟烷麻醉,置入可弯曲喉罩,保留自主呼吸.记录多时点气道峰压和平均气道压.记录气管导管或喉罩置人即刻(T1)、1 min(T2)、3 min(T3)、5 min(T4)和拔出气管导管或喉罩即刻(T5)时血压、心率.记录苏醒时间和拔管时间;患儿从苏醒到拔出气管导管或喉罩时呛咳、躁动和术后咽痛的情况及家长的满意度.结果 与基础值比较,气管导管组T1时收缩压下降,T2、T3、T5时收缩压升高,T2～T5时心率升高,可弯曲喉罩组T1～T4时收缩压、舒张压下降(P<0.05);与气管导管组比较,可弯曲喉罩组T2～T5时收缩压、舒张压、心率均降低(P<0.05).气管导管组苏醒时间(8.1±2.6)min,拔管时间(12.6±3.2)min,从苏醒到拔出气管导管或喉罩时呛咳发生率66.7%(20/30)、咽痛发生率40.0%(12/30)、躁动评分0～1分7例,可弯曲喉罩组分别为(5.2±1.1)min、(6.3±1.5)min、0、6.7%(2/30)、25例,两组比较差异有统计学意义(P<0.05).与气管导管组比较,可弯曲喉罩组患儿家长的满意度高,差异有统计学意义(P<0.05).结论 可弯曲喉罩通气下七氟烷麻醉更适合于小儿斜视矫治术.

Abstract：

Objective To evaluate the efficacy of flexible laryngeal mask airway and sevoflurane in pediatric strabismus surgery. Methods Totally 60 children undergoing elective strabismus surgery were recruited and divided into two groups with 30 cases each by random digits table:group T, in which children accepted endotracheal tube intubation and combined intravenous and inhalation anesthesia with mechanically ventilation; and group F, in which children adopted both flexible laryngeal mask airway and inhalation anesthesia of sevoflurane with spontaneous breath. Airway peak pressure (Ppeak), average airway pressure (Pmean), blood pressure (Bp) and heart rate (HR) at induction (T1), 1 min (T2), 3 min (T3) and 5 min (T4) after induction, removal of endotracheal tube or laryngeal mask airway (T5), awaking time and extubation time, occurrence of cough, dysphoria and pharyngodynia from being awake to extubation, satisfaction of patients were recorded. Results When compared to baseline, systolic pressure was lower at T, and higher at T2, T3 and T5, and HR was higher at T2-T5 in group T(P< 0.05),Bp was lower at T1-T4 in group F (P < 0.05). Bp and HR at T2-T5 in group F were lower than those in group T (P < 0.05). The awaking time was (8.1 ?2.6) min, the extubation time was (12.6 ?3.2) min, the occurrence of cough and pharyngodynia was 66.7%(20/30), 40.0%( 12/30), the dysphoria (0-1 score) was 7 cases in group T,(5.2 ± 1.1) min, (6.3 ± 1.5) rain,0,6.7%(2/30),25 cases in group F. There were significant differences between two groups (P<0.05). The satisfaction of patients in group F was higher than that in group T, there was significant difference between two groups (P< 0.05). Conclusion Flexible laryngeal mask airway with sevoflurane could offer high efficacy in pediatric strabismus surgery.     

高压氧联合化疗对卵巢上皮性癌疗效研究

目的 探讨高压氧联合PC方案化疗对卵巢上皮性癌初治患者的疗效.方法 将58例卵巢上皮性癌术后患者采用随机数字表法分为研究组(30例)和对照组(28例),对照组给予环磷酰胺1000 mg/m2+顺铂75 mg/m2,21 d为1个疗程,至少6个疗程.研究组在高压氧2个标准大气压下暴露60 min,出舱25～30 min给予对照组方法化疗.比较两组的近期疗效和3年存活率、无进展生存时间以及不良反应等.结果 研究组近期总有效率、未控率、复发率、复发时间、3年存活率分别为83.3%(25/30)、6.7%(2/30)、33.3%(10/30)、(21.0±0.8)个月、43.3%(13/30),明显优于对照组的67.9%(19/28)、17.9%(5/28)、46.4%(13/28)、(18.0±0.6)个月、17.9%(5/28)(P＜0.05).研究组无进展生存时间和总生存时间明显长于对照组(P＜0.05),且不良反应发生率明显低于对照组(P＜0.05).结论 卵巢上皮性癌的辅助化疗中,高压氧联合PC方案疗效优于单纯PC方案,且高压氧能明显减少PC方案不良反应.     

高效液相色谱法同时测定食品中的B族维生素

用HPLC法同时测定食品中的四种B族维生素(B_1,B_2,B_5,B_6)。实验用0.4mol/L盐酸,120℃、15psi水解样品。分析以μBondapakC18(300mm×3.9mm)为色谱柱,甲醇:重蒸水:庚烷磺酸(PIC-B_7)=30:68:2(V:V:V)为流动相,选择紫外254nm,对B_1、B_2和B_5以及荧光(EX295nm、EM395nm)对B_6的双通道检测,可得到很好的分离图形。B_1、B_2、B_5和B_6的线性范围与相关系数各为10～50ng(r=0.998),5～40ng(r=0.999),5～40ng(r=0.993),5～50ng(r=0.999),最小检出限各为5、5、2、2ng。回收率各为91.3％～102％,103％～105％,92％～100％和99％～100％。与参比实验相比,所得食物中B族维生素含量差异不大,并与食物成分表公布的数据十分接近。     

阿帕替尼联合培美曲塞在治疗铂耐药复发性卵巢癌中的应用

目的 探讨阿帕替尼联合培美曲塞在铂耐药复发性卵巢癌患者中的疗效。方法 收集2018年4月—2021年5月在本院就诊的30例铂耐药复发性卵巢癌患者的临床资料,分析阿帕替尼联合培美曲塞对晚期铂耐药复发性卵巢癌患者的疗效和不良反应等相关指标。结果 本研究最终入组30例患者,末次随访时间为2021年11月30日,客观缓解率为36.7%(11/30),疾病控制率为86.7%(26/30),中位无进展生存时间为9.2月 (95%CI为6.3～12.1月)。发生率较高的不良反应集中在1～2级,包括口腔黏膜炎为14例(46.7%)、高血压为14例(46.7%)、乏力为11例(36.7%)、手足综合征为5例(16.7%)、恶心呕吐5例(16.7%)、腹泻4例(13.3%)等;高级别不良反应发生2例,包括3级口腔黏膜炎1例、3级手足综合征1例。所有患者的不良反应经药物治疗后控制良好或无须特别处理。结论 阿帕替尼联合培美曲塞对铂耐药复发性卵巢癌患者疗效满意,且安全性可控。     

70例手足口病病原检测和临床分析

目的 分析研究70例手足口病患者的临床一般情况及其致病原.方法 采集70例手足口病患者(其中5岁以下儿童患者60例)的咽拭子标本,提取病毒RNA.用逆转录聚合酶链反应(RT-PCR)一步法检测肠道病毒(EV)的5'-UTR区基因、肠道病毒71型(EV71)的Vp3～Vp1区基因、柯萨奇病毒A16(Cox-A16)的Vp3～Vp1区基因.结果 在70例手足口病患者中,咽拭子标本肠道病毒核酸阳性率为42.8%(30/70).在30例肠道病毒核酸阳性病原中,EV71占66.7%(20/30).5岁以下儿童有EV71肠道病毒、或Cox-A16病毒、或非EV71及非Cox-A16的其他肠道病毒感染病例.而5岁以上的儿童和成人患者则只有EV71肠道病毒感染病例.39例4 d内采集的咽拭子标本病原核酸阳性检测率平均为66.7%(26/39),31例5 d以后采集的标本病原核酸阳性检测率是12.9%(4/31),差异有统计学意义(x~2=20.4,P<0.01).结论 手足口病以0～5岁婴幼儿患病为主,但成人也可发病.用RT-PCR检测病原核酸阳性的手足口病患者以EV71为主,且以发病后4 d内阳性检测率高.