酸性染料比色法测定阿昔洛韦

目的:建立比色法测定阿昔洛韦浓度的方法,并用于实际药品、血浆及尿液中阿昔洛韦的测定。方法:在 NaH_2PO_4-Na_2HPP_4缓冲介质中,阿昔洛韦(ACV)与酚红(PR)、氯酚红(CPR)反应,形成离子缔合物,溶液颜色发生明显改变,最大吸收波长分别为558 nm(PR—ACV 体系)、574 nm(CPR—ACV 体系),在此波长处阿昔洛韦的浓度与增色程度呈良好线性关系。结果:在 PR—ACV、CPR—ACV 体系的最大吸收波长处,ACV 的浓度分别在0～2.86×10~(-5)mol·L~(-1)、0～2.37×10~(-5)mol·L~(-1)范围内遵守比尔定律,表观摩尔吸光系数分别为1.77×10~4L·mol~(-1)·cm~(-1)、1.64×10~4L·mol~(-1)·cm~(-1),检出限分别为8.21×10~(-7)mol·L~(-1)、4.50×10~(-7)mol·L~(-1)。结论:方法具有较高的灵敏度和良好的选择性,用于实际药品、血浆及尿液中阿昔洛韦的测定,结果满意。     

双波长分光光度法测定甲硝唑呋喃西林外用片中甲硝唑的含量

目的:建立甲硝唑呋喃西林外用片中甲硝唑的含量测定方法.方法:采用双波长等吸收分光光度法,以0.1 mol·L-1的盐酸溶液为溶剂,在(402±1)nm和(276±1)nm波长处分别测定甲硝唑吸光度以供差值计算甲硝唑的含量,消除呋喃西林的干扰.结果:甲硝唑在1.01×10-3～1.51×10-5g·ml-1浓度范围内呈良好线性关系,r=0.999 9;平均回收率为99.12%～100.47%,RSD=0.62%(n=9).结论:该方法简便、快速、准确,适用于甲硝唑的含量测定.     

刚果红褪色光度法测定硫酸阿米卡星

在酸性条件下 ,刚果红 (CR)与硫酸阿米卡星 (AMK)反应 ,生成离子缔合物 ,使刚果红溶液褪色 ,最大褪色波长位于 5 6 4 nm,表观摩尔吸光系数 (ε)为 2 .6 1× 10 4 L/ (mol·cm) ;硫酸阿米卡星的浓度在 0～ 1.7× 10 - 5mol/ L 范围内 ,遵从比尔定律。该法用于市售药物中硫酸阿米卡星含量的测定 ,结果满意。     

二甲酚橙褪色分光光度法测定乳酸诺氟沙星含量

目的二甲酚橙褪色分光光度法测定乳酸诺氟沙星的含量。方法在碱性介质中,乳酸诺氟沙星与二甲酚橙染料反应,形成电荷转移化合物,使二甲酚橙溶液颜色发生明显改变,最大褪色波长在577nm,以此建立了测定乳酸诺氟沙星的褪色分光光度法。结果在pH7.0～12.0的碱性介质条件下,诺氟沙星与二甲酚橙形成2∶1型电荷转移化合物。在最大褪色波长处,乳酸诺氟沙星浓度在6.22×10^-6～9.33×10^-5mol/L范围内褪色程度与乳酸诺氟沙星浓度有良好的线性相关;精密度RSD为3.6%;方法回收率为（92.0±4.6）%。结论二甲酚橙褪色分光光度法操作简便,有较高的灵敏度和良好的选择性。用于乳酸诺氟沙星的含量测定,结果较为满意。     

阿昔洛韦软膏的含量测定

目的用紫外分光光度法测定阿昔洛韦软膏的含量,为该药提供质量控制方法。方法紫外分光光度法。结果阿昔洛韦在251.6nm处有最大吸收,在4～12μg.mL-1范围内吸收度与浓度有良好线性关系,其回归方程为：A=-0.0162＋0.0645C（r=0.9999）,平均回收率（n=6）为100.45%,RSD=0.67%。结论该法作为阿昔洛韦软膏的含量测定方法,快捷准确,简便易行,适用于医院快检。     

盐酸酚苄明片溶出度测定方法的研究

目的建立盐酸酚苄明片的溶出度测定方法。方法以0.1 mol.L-1盐酸溶液为溶出介质,转速为75r.min-1,紫外-分光光度法测定,检测波长267nm。结果盐酸酚苄明在10~946μg.mL-1的浓度范围内线性关系良好,线性方程为A=8.02×10-2C+3.4×10-2(r=0.9999)。结论本方法操作简便,结果准确,可用于完善盐酸酚苄明片的质量标准。     

分光光度法测定甲紫溶液含量的再探讨

报道以紫外分光光度法测定甲紫溶液含量测定的结果。甲紫溶液在 0 5～ 4mg·L-1浓度范围内 ,波长范围 45 0～6 5 0nm间 ,于 5 85± 1nm处有最大吸收 ,浓度与吸收度线性关系良好。样品回收率平均为 98 5 6 % ,RSD 3 5 7%。方法简便可靠 ,与重量分析法比较误差较小     

络合分光光度法测定卡铂抗肿瘤膜剂含量

目的 建立络合分光光度法测定膜剂中卡铂含量的方法.方法 用蒸馏水提取膜剂中卡铂,与等体积1mol·L-1 SbCL2 的 1mol·L-1盐酸溶液反应后,用络合分光光度法测定反应液吸收度,检测波长为395 nm.结果 反应液中卡铂浓度于7.67～46.00 μg·mL-1之间与其吸收度有良好线性关系,r=0.9993;平均回收率=100.3%,RSD=2.3%(n=6).结论 该方法简便、快速、准确,适于测定膜剂中卡铂含量及质量控制使用.     

紫外分光光度法测定盐酸头孢他美特戊酯片的含量

目的建立紫外分光光度法测定盐酸头孢他美特戊酯片含量的方法.方法用0.1 mol·L-1盐酸为溶剂,在266nm波长处测定含量.结果盐酸头孢他美特戊酯浓度在4.42～26.6 mg·L-1范围内与吸收度呈良好的线性关系,相关系数r=0.9999,平均回收率为99.4%,RSD=0.3%(n=6).结论紫外分光光度法操作快速、简便,结果准确,适合于该制剂的含量测定.     

HPLC法测定阿昔洛韦缓释片的含量

目的HPLC法测定阿昔洛韦缓释片的含量。方法采用HPLC法。色谱柱为HypersilODS 5μm 4.0×250mm;流动相:甲醇-水(10∶90);检测波长254nm;流速:1mL.m in-1。结论本方法预处理时间短、准确性好、方法简单。结果阿昔洛韦的浓度在2.5μg.mL-1~30μg.mL-1范围内,峰面积和浓度线性关系良好。精密度测试,重复进样6次。RSD 0.57%。回收率试验平均99.99%,RSD 0.23%,溶液较稳定。     

抗人绒毛膜促性腺激素β—亚基单克隆抗体杂交瘤的建立

用绒毛膜促性腺激素（HCG）及其β亚基（HCG-β）脾内免疫BALB/C小鼠后，取脾细胞与sp2/o小鼠骨髓瘤细胞融合，其融合剂PEG终浓度为37%，融合率100%，经反复筛选，克隆化后，获得三株持续分泌抗HCG-β单克隆抗体杂交瘤-E2、E12和F6，经亚型鉴定E2和E12为IgG1,F6为IgG2b。该杂交瘤所分泌的抗体公能与HCG和HCG-β特异性结合，而不与促共同体素（hLH)、促卵泡素（hFSH)和促甲状腺素（hTSH)发生交叉反应，并经ELISA抑制试验证实，单克隆抗HCG-β具有高度的特异性，可以为早孕和妇科肿瘤的特异性诊断提供原料，本文还对免疫程序各融合方法进行了讨论。     

PHARMACOLOGICAL COMPARISON OF HUMAN ISOLATED DIGITAL ARTERIES AND METACARPAL VEINS

1 The responses of human digital arteries and metacarpal veins obtained postmortem to various pharmacological agents have been tested. 2 pD₂ values for potassium chloride and barium chloride were found to be greater in arteries than in veins. 3 There was no difference between the arteries and veins in the pA₂ values for phentolamine as an antagonist of either noradrenaline or phenylephrine. pD₂ values for noradrenaline however, were significantly higher in the veins than in the arteries, whereas pD₂ values for phenylephrine in the two tissues were not significantly different. This raises the possibility of there being differences in the populations of a-adrenoceptors in the two tissues. 4 Differences were found between arteries and veins in the contractile and relaxant responses to histamine and in the antagonism of the responses to histamine by cimetidine and mepyramine, thereby suggesting differences in the populations of Hi-and H₂-receptors in these tissues. 5 No differences were found in the responses of arteries and veins to serotonin or in the antagonism of the response to this agonist by phentolamine. 6 Isoprenaline produced relaxant responses in veins (in which tone was induced with 30 mmol/1 potassium chloride) but not in arteries. 7 Dopamine produced very weak relaxant responses in preparations in which tone was induced using 30 mmol/1 potassium chloride. The mean £max value for this response was significantly greater in veins than in arteries. 8 Slight relaxant responses to acetylcholine were seen in veins and arteries precontracted with 30 mmol/1 potassium chloride. The mean £max value was significantly greater in veins than in arteries. 9. It is concluded that human digital arteries and metacarpal veins have differing pharmacological receptor populations and probably also differ in their non-receptor mediated contractile mechanisms.     

国产冻干人凝血酶原复合物质量标准的研究

目的通过对国产人凝血酶原复合物 (PCC)质量的研究 ,建立与国际接规的质量标准。方法用人凝血因子Ⅱ、Ⅶ、Ⅸ、Ⅹ (FⅡ、FⅦ、FⅨ、FⅩ )浓制剂国家标准品 ,采用一期法测定国内用不同原料制备的PCC中FⅡ、FⅦ、FⅨ、FⅩ的效价 ;用正常人血浆作标准 ,用凝固法检测PCC的总效价及PCC中的肝素。结果用血浆作原料提纯制备的PCC ,FⅣ的效价能达到 1 0IU/ml以上 ,FⅦ效价最低 ;用组分Ⅲ作原料 ,FⅣ活性损失很大 ,只有 2 / 7批次能达到 1 0IU/ml;而FⅦ效价最高。无论用何种原料 ,FⅡ和FⅩ活性均能保持在一定水平。结论从血浆中直接提纯制备PCC ,能有效地保持FⅣ活性 ,各项指标易达到国外同品种的质量标准     

论人体生命的三重品格

本文讨论的是人体生命观的问题,从人的本质来看,人体生命与其它生命体有着原则的区别。人体生命是神圣品格、质量品格和价值品格的辩证统一。人体生命三重品格以价值品格为核心,密不可分地联系在一起。片面强调人体生命的质量品格或神圣品格,必然使人们在理论和实践上陷入二难境地。     

Human embryonic stem cells and lung regeneration

Human embryonic stem cells are pluripotent cells derived from the inner cell mass of preimplantation stage embryos. Their unique potential to give rise to all differentiated cell types has generated great interest in stem cell research and the potential that it may have in developmental biology, medicine and pharmacology. The main focus of stem cell research has been on cell therapy for pathological conditions with no current methods of treatment, such as neurodegenerative diseases, cardiac pathology, retinal dysfunction and lung and liver disease. The overall aim is to develop methods of application either of pure cell populations or of whole tissue parts to the diseased organ under investigation. In the field of pulmonary research, studies using human embryonic stem cells have succeeded in generating enriched cultures of type II pneumocytes in vitro. On account of their potential of indefinite proliferation in vitro, embryonic stem cells could be a source of an unlimited supply of cells available for transplantation and for use in gene therapy. Uncovering the ability to generate such cell types will expand our understanding of biological processes to such a degree that disease understanding and management could change dramatically.     

Human Herpesvirus 6 (HHV-6) Induces Dysregulation of Glutamate Uptake and Transporter Expression in Astrocytes

Human herpesvirus 6 (HHV-6) infects and establishes latency in the central nervous system (CNS). Reactivation of latent HHV-6 has been associated with neurologic diseases including epilepsy and multiple sclerosis (MS). In vivo, HHV-6 has been localized to astrocytes and can infect human astrocytes in vitro, suggesting that this virus may have a tropism for glial cells and may affect glial cell function. An essential role of astrocytes in the CNS is active maintenance of the excitatory neurotransmitter glutamate. Dysregulation of glutamate has been implicated as a potential mechanism of disease in both epilepsy and MS. Both disorders have demonstrated elevated glutamate in CSF and may be associated with dysregulation of glutamate signaling, uptake, and metabolism. This study demonstrates dysregulation of glutamate uptake in human astrocytes infected with both variants of HHV-6, A and B, with differential effects of HHV-6 in acute and persistently infected cells. Whereas astrocytes acutely infected with HHV-6 demonstrated increased glutamate uptake, cells persistently infected with HHV-6A and HHV-6B demonstrated impaired glutamate uptake. Functional dysregulation of glutamate uptake was associated with early increases in mRNA and protein expression of the glial glutamate transporter EAAT-2 followed by a sustained decrease in mRNA expression in astrocytes infected with both HHV-6A and HHV-6B. Dysregulated glutamate uptake and transporter expression suggests a mechanism for dysregulation of glutamate levels in vivo and a potential mechanism for virus-associated neurologic disease. This work was supported by the National Institutes of Health/National Institute of Neurological Disorders and Stroke. JF was supported by the MS Society of Canada.     

Resolution of human mercapt- and nonmercaptalbumin by high-performance liquid chromatography

Gel-exclusion high-performance liquid chromatographic (HPLC) analysis of human serum albumin (HSA) on PGP 2000 column (0.10 M sodium phosphate buffer, 0.30 M NaCl, pH 6.86) showed at least two peaks, the principal component corresponding to human mercaptalbumin (HMA) and the second one to human nonmercaptalbumin (HNA). Mechanism for the separation of HMA and HNA might be due to weak resin-HSA interaction. HPLC analysis of bovine plasma albumin (BPA) showed a single peak on PGP 2000 column. The elution volume of HSA was larger than that of BPA, resulting in a clear resolution of HSA and BPA.     

Further studies on the resolution of human mercapt- and nonmercaptalbumin and on human serum albumin in the elderly by high-performance liquid chromatography

High-performance liquid chromatographic (HPLC) analysis of human serum albumin (HSA) on Asahipak GS-520 showed at least two peaks, the principal component corresponding to human mercaptalbumin (HMA) and the secondary one to nonmercaptalbumin (HNA). HPLC analysis of HSA on Asahipak ES-520 N showed three peaks, the principal component corresponding to HMA, the secondary one to HNA having mixed disulfide with cysteine or glutathione and the tertiary one to HNA oxidized higher than mixed disulfide. Two kinds of rapid HPLC for the resolution of HSA into HMA and HNA were developed by the present authors. Using these HPLC, the present authors found a significant decrease in the fraction of HMA in the elderly.     

首批人凝血因子Ⅱ和Ⅹ浓制剂国家标准品协作标定

目的协作标定首批人凝血因子Ⅱ和Ⅹ浓制剂国家标准品。方法用世界卫生组织 98 590批人凝血因子Ⅱ和Ⅹ浓制剂国际标准品 ,采用一期法标定我国首批人凝血因子Ⅱ和Ⅹ浓制剂国家标准品 ,将标准品分别于4℃、2 2℃、37℃保存 5个月 ,进行稳定性考查。结果人凝血因子Ⅱ和Ⅹ国家标准品的效价分别为 1 6 .1IU 支和 1 2 .1IU 支 ,稳定性良好。结论确定了首批人凝血因子Ⅱ和Ⅹ浓制剂的国家标准品     

Carbonic Anhydrase Inhibition with Benzenesulfonamides and Tetrafluorobenzenesulfonamides Obtained via Click Chemistry

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