Two new diterpenoids from Mallotus apelta Muell.Arg

Two new diterpenoids, malloapeltene (6,8-dihydroxy-cembrene-5-one) and malloapeltin (4alpha,15,16-trihydroxy-dolabradane) were isolated and characterized from the petroleum ether fraction of the alcoholic extract of Mallotus apelta Muell.Arg. Their structures were determined by spectral methods.     

Two New Diterpenoids from Mallotus apelta Muell.Arg.

Abstract

Two new diterpenoids, malloapeltene (6,8-dihydroxy-cembrene-5-one) and malloapeltin (4α,15,16-trihydroxy-dolabradane) were isolated and characterized from the petroleum ether fraction of the alcoholic extract of Mallotus apelta Muell.Arg. Their structures were determined by spectral methods.     

Three new diterpenoids from Aralia dumetorum

Three new diterpenoids, dumetoranes A (1) and B (2), melanocane B (3), together with four known ones including melanocane A (4), ent-15S,16-dihydroxypimar-8(14)-en-19-oic acid (5), ent-pimara-8(14),15-diene-19-oic acid (6), and ent-pimara-8(14),15-diene-19-ol (7) were obtained from the ethanol extract of the roots of Aralia dumetorum. Their structure elucidation was achieved by the methods of spectroscopic HRMS, IR, NMR, and by comparison with literature. The cytotoxicities of compounds 1–3 and 5 were assayed by in vitro MTT methods.     

锈毛野桐中的两个新二萜anomalusin A和anomalusin B(英文)

从锈毛野桐中分离得到两个新的ent-rosane型二萜anomalusin A(1)和anomalusin B(2),以及10个已知化合物。化合物1和2的结构和相对构型通过波谱学方法确定,它们的绝对构型通过CD谱确定。     

Three new diterpenoids from Coleus forskohlii Briq

Three new diterpenoids, forskolin G(2), forskolin H(3), forskolin I(4), were isolated from the whole plant of the Coleus forskohlii Briq., and their structures were elucidated as 1α,6β-diacetoxy-8,13-epoxylabd-14-en-11-one, 1α-hydroxy-6β,7β-diacetoxy-8,13-epoxylabd-14-en-11-one, and 1α,9α-dihydroxy-6β,7α-diacetoxy-8,13-epoxylabd-14-en-11-one on the basis of spectral data.     

Three new jatrophone-type diterpenoids from Euphorbia helioscopia

Three new jatrophone-type diterpenoids, euphoheliosnoids A-C, together with two known related compounds, were isolated from the Chinese medicinal plant Euphorbia helioscopia L. The structures including relative stereochemistry of the new compounds were elucidated by spectroscopic methods and by comparison with known related compounds.     

白背叶根对过氧化氢所致大鼠肝细胞损伤的保护作用

目的　探讨中药白背叶根对过氧化氢 (H2 O2 )所致大鼠肝细胞损伤的影响。方法　用终浓度为 2mmol·L-1的H2 O2 对大鼠肝细胞进行损伤 ,收集肝细胞悬液 ,测定丙氨酸转氨酶 (ALT)、丙二醛 (MDA)、超氧化物歧化酶 (SOD)和一氧化氮 (NO)的含量。结果　白背叶根作用肝细胞后 ,能显著降低H2 O2 引起的NO和MDA水平的升高 ,并提高SOD活性 ,显著降低肝细胞悬液中ALT的浓度。结论　白背叶根对H2 O2 所致的大鼠肝细胞氧化损伤有保护作用。     

Three new isopimarane diterpenoids from Excoecaria acerifolia

Three new isopimarane diterpenoids named excoecarins F–H (1–3) were isolated from the EtOAc extract of the Chinese ethnodrug Gua-jing-ban (Excoecaria acerifolia Didr.). Their structures were elucidated by the analysis of spectroscopic data including 1D, 2D NMR and HR-MS. The anti-HIV-1 bioactivity test of 1 and 2 showed weak activity.     

Three new diterpenoids from Coleus forskohlii Briq

Three new diterpenoids, forskolin G(2), forskolin H(3), forskolin I(4), were isolated from the whole plant of the Coleus forskohlii Briq., and their structures were elucidated as 1alpha,6beta-diacetoxy-8,13-epoxylabd-14-en-11-one, 1alpha-hydroxy-6beta,7beta-diacetoxy-8,13-epoxylabd-14-en-11-one, and 1alpha,9alpha-dihydroxy-6beta,7alpha-diacetoxy-8,13-epoxylabd-14-en-11-one on the basis of spectral data.     

GC-MS测定白背叶中的挥发油

目的 分析白背叶挥发油的化学成分并用归一化法测定各成分的质量分数.方法 采用水蒸气蒸馏法提取白背叶中的挥发油.用GC-MS法鉴定其化学成分.结果 共鉴定了41个成分,占挥发油总成分的90%以上.结论 白背叶挥发油的主成分为橙花叔醇、1,6-辛二烯-3-醇、冰片基胺、己二酸二异辛酯和2,7-二甲基-1,6-辛二烯.     

Three new acyclic diterpenoids from Eupatorium lindleyanum DC

Three new acyclic diterpenoids were isolated from the whole plant of Eupatorium lindleyanum DC. The structures of the compounds were elucidated by means of (1)H and (13)C NMR spectroscopic analyses, including 2D NMR experiments.     

白背叶叶中总黄酮含量的测定

目的：探索白背叶叶总黄酮的最佳提取方法,并建立白背叶叶提取物中总黄酮含量的测定方法。方法：利用NaNO2-Al（NO3）3—NaOH显色、分光光度法测定白背叶叶中黄酮类物质含量．检测波长为510nm。结果：芦丁检测浓度在0．004～0．02mg·mL^-1范围内与吸光度线性关系良好（r=0．9998）,加样平均回收率为100．15％,RSD=1．01％（n=5）．用乙醇提取法提取的叶中总黄酮含量可达到0．024％。结论：本方法简便、快速、准确。而且对仪器设备的要求不高,操作简单易行．适用于黄酮类物质的含量测定。     

ICP-MS法同时测定广西白背叶药材中30种微量元素的含量

目的:建立同时测定广西白背叶药材中30种微量元素含量的方法。方法:采用电感耦合等离子体质谱法。射频功率为1 550 W,采样深度为10 mm,载气流速为1.05 L/min,雾化室温度为2℃,蠕动泵频率为0.10 rps,碰撞模式为氦,气流量为4.2 m L/min。结果:铝、钒、铬、锰、铁、镍、铜、锌、镓、砷、铷、钼、镉、锑、铯、钡、镧、铈、镨、钕、铕、钆、镝、钬、铒、镥、汞、铊、铅、铀检测质量浓度线性范围分别为10～1 000 ng/mL(r=0.999 9)、0.1～5 ng/mL(r=0.999 9)、0.1～5 ng/mL(r=0.999 9)、10～1 000 ng/mL(r=0.999 9)、10～1 000 ng/mL(r=0.999 9)、0.1～5 ng/mL(r=0.999 7)、0.5～100 ng/mL(r=0.999 7)、1～500 ng/mL(r=0.999 8)、1～100 ng/mL(r=0.999 7)、0.5～50 ng/mL(r=0.999 9)、0.5～50 ng/mL(r=0.999 9)、0.1～10 ng/mL(r=0.999 9)、0.1～10 ng/mL(r=0.999 9)、0.1～10 ng/mL(r=0.999 9)、0.1～10 ng/mL(r=0.999 9)、1～500 ng/mL(r=0.999 9)、0.1～10 ng/mL(r=0.999 9)、0.1～10 ng/mL(r=0.999 9)、0.1～10 ng/mL(r=0.999 9)、0.1～10 ng/mL(r=0.999 8)、0.1～10 ng/mL(r=0.999 9)、0.1～10 ng/mL(r=0.999 9)、0.1～10 ng/mL(r=0.999 9)、0.1～10 ng/mL(r=0.999 9)、0.1～10 ng/mL(r=0.999 9)、0.1～10 ng/mL(r=0.999 8)、0.1～5 ng/mL(r=0.998 4)、0.1～10 ng/mL(r=0.999 9)、0.5～100 ng/mL(r=0.999 7)、0.1～10 ng/mL(r=0.999 9),定量限≤0.44 mg/kg,检测限≤0.11 mg/kg;精密度、稳定性、重复性试验的RSD≤10.0%;加样回收率为80.45%～116.68%(RSD为0.47%～5.83%,n=6)。药材样品中Mn、Ba、Fe、Al的含量最高。结论:该方法操作简便,精密度、稳定性、重复性好,可用于广西白背叶药材中30种微量元素含量的同时测定;药材中有害元素的含量符合国家相关标准。     

Three new diterpenoids from the leaves of Thuja orientalis

Three new diterpenoids, 18-formyloxy-8 β-hydroxysandaracopimar-15-ene (1), 15(R)-N-butoxypinusolidic acid (2), and 15,16-dihydro-15,16-dimethoxylambertianic acid (3), along with twelve known compounds (4-15) were isolated from MeOH extracts of leaves of Thuja orientalis L. The structures of the three new compounds were elucidated on the basis of spectroscopic analyses, including extensive 2D-NMR data. The absolute stereochemistry of compound 2 was clarified by a CD spectroscopic study. The isolated compounds were evaluated for cytotoxicity against A549, SK-OV-3, SK-MEL-2, and HCT-15 cell lines in vitro using the sulforhodamin B (SRB) bioassay.     

白背叶黄酮类化合物抗鸭乙型肝炎病毒活性研究

目的:研究白背叶黄酮类化合物(WF)抗鸭乙型肝炎病毒(DHBV)的活性。方法:对1d龄雏鸭人工感染DHBV,7d后成功复制鸭DHBV感染模型。分别灌胃75、150mg·kg-1的WF,或50mg·kg-1拉米夫定,每天2次,连续给药10d。斑点杂交法测定给药第5天、第10天及停药后第3天鸭血清中DHBV-DNA浓度。结果:75及150mg·kg-1WF均能显著降低模型鸭血清中DHBV-DNA浓度(P<0.01),且停药后反跳程度轻于拉米夫定。结论:WF有显著的抑制DHBV-DNA复制的作用,表明其可能有较好的抗乙型肝炎病毒的活性。     

Three new pimaren diterpenoids from marine mangrove plant, Bruguiera gymnorrhiza

Three new pimaren diterpenoids, ent-8(14)-pimarene-15R, 16-diol (1), ent-8(14)-pimarene-1alpha,15R,16-triol (2), and (5R, 9S, 10R, 13S, 15S)-ent-8(14)-pimarene-1-oxo-15R,16-diol (3), along with three known diterpenoids (4-6) have been isolated from the stem of mangrove plant Bruguiera gymnorrhiza. The structures of compounds 1-3 were determined by extensive spectroscopic (2D NMR, MS, IR, and CD) analysis, and 3 and 5 showed moderate cytotoxic activities against L-929 and K562, respectively.     

Three tricyclic diterpenoids from Euphorbia decipiens

Three new diterpene esters with a tricyclic lathyraneor myrsinol-type skeleton have been isolated from Euphorbia decipiens Boiss & Buhse. The structures have been elucidated by different spectroscopic methods including 1D and 2D NMR spectroscopy techniques.     

Four new diterpenoids from Salvia fulgens Cav.

Four new diterpenoids, salvifulgenolide (1), trans-1,2-dihydrosalvifaricin (2), ent-19- O-acetoxy-15,16-epoxy-3,13(16),14-clerodatrien-6,18-diol (3) and ent-19-acetoxy-15,16-epoxy-6-hydroxy-3,13(16),14-clerodatrien-18-al (4) along with two known diterpenoids, 10-hydroxybacchotricuneatin A (5) and dehydrokerlin (6) were isolated from the aerial parts of Salvia fulgens Cav. (Labiatae). The structures were established by spectroscopic methods, including the X-ray analysis of salvifulgenolide and trans-1,2-dihydrosalvifaricin.     

Two new diterpenoids from Coleus forskohlii

Two new diterpenoids, forskolins I and J, have been isolated in our further investigation on Coleus forskohlii (Willd.) Briq. collected in Yunnan Province. Their structures have been determined as 1alpha,6beta-diacetoxy-7beta,9alpha-dihydroxy-8,13-epoxylabd-14-en-11-one (1) and 1alpha,9alpha-dihydroxy-6beta,7beta-diacetoxy-8,13-epoxylabd-14-en-11-one (2) by spectral methods (including 1D and 2D NMR techniques).