Mirizzi综合征36例临床诊断与治疗体会

目的：探讨Mirizzi综合症的诊断治疗特点,以提高其诊断和治疗水平。方法：对36例经手术证实为Mirizzi综合症的临床资料进行回顾性分析。结果：36例Mirizzi综合症患者中仅4例（11.1%）术前确诊。按Csendes分型,Ⅰ型7例,Ⅱ型18例,Ⅲ型9例,Ⅳ型2例。5例行胆囊切除术,2例行胆囊大部分切除术;胆囊切除、瘘口修补10例,13例行胆囊切除、胆总管探查、瘘口修补、T管引流术;胆囊切除、胆肠吻合术5例。术后恢复好,随访无严重并发症。结论：Mirizzi综合症术前确诊困难,B超结合MRCP/ERCP检查可以提高Mirizzi综合症的术前确诊率,手术容易损伤胆管,手术方式应据病理损伤程度决定。     

Mirizzi综合征14例的手术治疗体会

目的探讨Mirizzi综合征的诊断及治疗。方法回顾性分析14例Mirizzi综合征的临床资料。结果14例均经手术证实,其中术前确诊6例,术中确诊8例,Ⅰ型7例,Ⅱ型5例,Ⅲ型2例。手术方式分别采用胆囊切除术,胆囊部分切除术,胆囊切除加胆总管探查T管引流或胆肠内引流术。结论Mirizzi综合征术前诊断困难,对不同病理类型的病例应采取不同的手术方法。     

Mirizzi综合征的临床诊治研究

目的探讨Mirizzi综合征的诊断治疗特点,提高诊断治疗水平。方法对1995年5月￣2006年8月经手术证实的Mirizzi综合征35例的临床资料作回顾性分析。结果35例Mirizzi综合征中仅1例(2.86%)术前确诊。35例中行胆囊切除术11例(31.43%),胆囊大部分切除术3例(8.57%),胆囊切除、胆囊瓣瘘口修补术5例(14.29%),胆囊切除胆总管探查、瘘口修补、"T"管引流术15例(42.86%),胆囊切除、胆总管切开取石、Roux-en-Y胆肠吻合术1例(2.86%)。结论Mirizzi综合征术前确诊困难,术中易损伤胆管。B超和ERCP检查相结合可提高术前确诊率,手术方式应根据病理损伤程度决定。     

Mirizzi综合征26例诊治分析

目的:探讨Mirizzi综合征的临床特点,总结Mirizzi综合征的诊断和治疗方法。方法:回顾性分析了26例经手术证实的Mirizzi综合征的临床资料,探讨其临床特点和手术方法。结果:术前确诊仅3例(11.5%),其中2例经ERCP确诊,1例经MRCP确诊。26例分别施行了胆囊切除术,胆囊大部切除术,胆囊瓣瘘口修补加"T"管引流术以及胆肠Rouxen-Y吻合术。25例术后一期愈合,1例并发胆漏再次手术。结论:Mirizzi综合征术前诊断困难,术中极易损伤胆管,手术探查应认真仔细,治疗应根据不同类型选择适当的手术方式。     

Mirizzi综合征25例临床治疗体会

目的:总结Mirizzi综合征的诊断与治疗特点。方法:对我院经手术证实的25例Mirizzi综合征患者的资料进行回顾性分析。结果:25例均采用手术治疗。Csendes分型:Ⅰ型13例,Ⅱ型7例,Ⅲ型3例,Ⅳ型2例。手术方式分别采用胆囊切除术、胆囊大部分切除术、胆囊切除加胆总管或肝总管探查术、肝圆韧带修复术、胆肠吻合术,其中22例行OC,术中无1例胆管损伤,但有1例术后局部疤痕增生,肝总管狭窄,胆管炎反复发作,予再次手术后愈合。2例行LC,其中1例因胆管损伤,而中转开腹行胆肠吻合术后治愈;1例行内镜治疗,效果满意。结论:Mirizzi综合征术前诊断较困难,B超结合CT、ERCP、MRCP检查可提高术前确诊率,防止术中胆管损伤的发生,治疗应根据不同类型选择适当的手术方式。     

Mirizzi综合征的诊断与治疗

目的总结Mirizzi综合征的诊断和治疗经验,探讨其临床特点,提高对该病的认识,避免胆管损伤。方法回顾性分析笔者所在医院2000年3月～2010年3月经手术证实的57例Mirizzi综合征患者的临床资料。结果 I型31例,行单纯胆囊切除术;Ⅱ型16例,行胆囊切除胆道探查术+T管引流;Ⅲ型10例,行胆囊切除+胆道探查+肝圆韧带瘘口修补+T管引流术。57例患者中15例行腹腔镜手术,3例术中解剖困难及出血而中转开腹,2例术中损伤胆道而中转开腹。术后随访时间为3个月～6年,平均3.5年,仅1例胆漏、1例出血而再次手术止血,其余患者均恢复良好。结论 Mirizzi综合征术前确诊较困难,治疗应根据病理损害程度选择适当的手术方式。     

Mirizzi综合征诊治体会

目的探讨Mirizzi综合征的诊断与治疗特点,提高其诊治水平。方法对经手术证实的17例Mirizzi综合征患者的临床资料进行回顾分析。结果17例Mirizzi综合征患者中仅1例(5.88%)术前确诊。术式选择分别为胆囊切除术9例(52.94%),胆囊大部分切除术4例(23.53%),胆囊切除、胆总管探查、瘘口修补、T管外引流术3例(17.65%),胆囊切除、胆总管探查、胆肠内引流术1例(5.88%)。17例中4例由于腹腔镜手术中解剖困难、出血,中转开腹。结论Mirizzi综合征术前诊断困难,治疗上应根据不同类型选择适当的手术方式,腹腔镜不宜为Mirizzi综合征的适应证。     

Mirizzi综合征的诊治18例报告

目的 探讨Mirizzi综合征的诊断及手术方法。方法 回顾分析1988年1月至2003年1月我院收治的18例Mirizzi综合征的诊断及治疗情况。结果 18例均行手术治疗，手术方式分别采用胆囊切除术、胆囊大部分切除术、胆囊切除加胆管T管引流术以及胆囊切除加胆肠内引流术。其中治疗满意16例，发生胆漏及胆管狭窄各1例，经保守治疗，效果良好。结论 不同类型Mirizzi综合征，应采取不同的手术方法。     

Mirizzi综合征的临床诊治分析

目的:探讨Mirizzi综合征的临床特点、影像学特征、诊断及治疗方法。方法:对13例Mirizzi综合征患者的临床资料进行回顾性分析。结果:69.2%(9/13)的患者其临床表现为右上腹疼痛伴有寒战发热、有黄疸病史。76.9%(10/13)的患者术前B超提示胆囊管或胆囊颈部存在结石嵌顿,其中6例行磁共振胆胰管造影(MRCP),提示胆囊结石、胆总管上段梗阻伴肝内胆管扩张。采用Csendes分型:Ⅰ型2例,行保留胆囊颈部的胆囊大部切除术;Ⅱ型4例,行胆囊大部切除术,瘘口直接缝合修补、T管引流;2例Ⅲ型患者,行胆囊部分切除术,残留的胆囊壁修补瘘口、T管引流;4例Ⅲ型患者和1例Ⅳ型患者,行胆囊大部切除术后,作胆肠Roux-en-Y吻合。结论:Mirizzi综合征患者的临床特点为右上腹疼痛多伴有寒战发热、有黄疸病史。术前B超提示胆囊管或胆囊颈部存在结石嵌顿、肝内外胆管扩张、胆总管内无明显结石的患者,应加行MRCP以提高术前诊断率。目前手术仍是确诊的主要手段,术中应根据病理分型采取相应的手术方式。     

Mirizzi综合征的腹腔镜治疗

目的:探讨腹腔镜胆囊切除术对Mirizzi综合征的效果及术中处理方法.方法:对采用LC治疗的16例Mirizzi综合征病例进行回顾性研究.结果:16例采用腹腔镜手术治疗,Ⅰ型12例行胆囊切除术,Ⅱ型2例行胆囊大部切除术,Ⅲ型1例行T管引流术,Ⅳ型1例行胆肠Roux-en-Y吻合.结论:在熟练掌握腹腔镜的前提下,腹腔镜治疗Mirizzi综合征的Ⅰ型、Ⅱ型是安全可行的方法.     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.