窒息新生儿脐带血血小板膜糖蛋白CD62p,CD61及CD41含量测？…

目的 探讨不同程度窒息新生儿脐带血血小板膜糖蛋白ＣＤ６２ｐ，ＣＤ６１及ＣＤ４１的改变及其临床意义。方法 以三种荧光单克隆抗体ＣＤ６２ｐ，ＣＤ６１及ＣＤ４１标记脐带血血小板，用流式细胞仪检测。     

米索前列醇用于足月妊娠引产1828例疗效观察

综合报道国内１６所医院分娩的初产妇１８２８例，随机分为两组，分别给予米索前列醇和催产素进行足月妊娠引产的病例对照研究。结果：米索前列醇用于足月妊娠的有效率为９３．５６％，显著高于催产素组７９．４９％（Ｐ＜０．０１），其临产发动时间及总产程分别为（２．９５±０．５３）ｈ和（６．３５±２．２１）ｈ，短于催产素组的（３．７５±０．６１）ｈ及（９．０９±２．４１）ｈ（Ｐ＜０．０１）；剖宫产率为８．８９％亦显著低于催产素组１７．０５％（Ｐ＜０．０１）；两组新生儿体重及新生儿窒息发生率均无显著差异（Ｐ＞０．０５）。因此认为：米索前列醇用于足月妊娠引产能缩短产程，降低剖宫产率，有利于计划分娩，是一种安全有效的引产方法。     

正常妊娠妇女外周血可溶性白细胞介素－2受体及淋巴细胞亚群的变化

测定了８７例妊娠晚期及２９例正常非孕妇女外周血可溶性白细胞介素－２受体（ｓＩＬ－２Ｒ）水平，同时对其中３６例孕妇及１０９例正常非孕妇女（正常对照）进行外周血淋巴细胞亚群检测。结果：妊娠晚期妇女ｓＩＬ－２Ｒ水平及Ｔｓ细胞（ＣＤ＿８）明显高于正常对照，分别为：２１４６００±７０４００Ｕ／Ｌ比１６２１００±８４１Ｄ０Ｕ／Ｌ，　Ｐ＜０．０１及３７．６％±５．３％比３１．３％±７．０％，Ｐ＜０．０１。妊娠妇女Ｔｈ细胞／Ｔｓ细胞（ＣＤ＿４／ＣＤ＿８）比例明显低于正常对照（１．２±０．２比１．５±０．５，Ｐ＜０．０１）。但总Ｔ淋巴细胞（ＣＤ＿３），ＣＤ＿４，细胞与正常对照相比，差异无显著性，分别为：６４．１％±７．３％比６６．０％±９．９％，Ｐ＞０．０５及４４．１％±５．８％比４３．８％±９．Ｏ％，Ｐ＞０．０５。相关分析表明孕妇ｓＩＬ－２Ｒ水平与ＣＤ＿３、ＣＤ＿４、ＣＤ＿８细胞及ＣＤ＿４／ＣＤ＿８均无显著相关性（ｒ分别为０．２０３２，０．２０７７，０．１０３７及０．１２１４，Ｐ均＞０．０５）。提示：孕妇外周血Ｔ淋巴细胞亚群及血清ｓＩＬ－２Ｒ的变化对维持正常妊娠有重要作用，ｓＩＬ－２Ｒ可能是促进胎儿正常生长的重要介质之一。     

妊娠期蛋白尿与血小板计数、平均体积和膜糖蛋白的关系

目的：了解妊娠期尿蛋白与血小板活化的关系及在妊高征发病中的作用。方法：用血液自动分析仪、流式细胞仪检测４２例正常非孕妇女，７１例正常妊娠妇女，３９例妊高征妇女的血小板计数，平均血小板体积（ＭＰＶ），活化血小板膜分子标志物ＣＤ６２、ＣＤ６３，２４小时尿蛋白定量。结果：妊娠期尿蛋白与ＢＰＣ、ＭＰＶ、ＣＤ６２、ＣＤ６３无相关（Ｐ＞０．０５），妊高征患者上述值明显高于正常非孕组与正常妊娠组（Ｐ＜０．０１）。尿蛋白与ＭＰＶ、ＣＤ６２、ＣＤ６３呈正相关（Ｐ＜０．０５）。结论：血小板活化参与妊娠期肾脏损害，肾血栓形成是尿蛋白增多的重要原因，慢性弥漫性血管内凝血参与妊高征形成     

卵巢癌可溶性抗原和抗CD3单克隆抗体诱导细胞毒性T细胞抗人卵巢癌的实验研究

目的探讨卵巢癌过继细胞免疫治疗新方法。方法提取卵巢癌细胞（ＣＯＣ１和ＣＯＣ２ｎ）可溶性抗原（ＴＳＡ），用ＴＳＡ和抗ＣＤ３单克隆抗体（ＣＤ３ＭｃＡｂ）共同诱导正常人外周血单个核细胞（ＰＢＭＣ）产生细胞毒性Ｔ细胞（ＣＴＬ），用ＣＴＬ于体外杀伤ＣＯＣ１细胞和裸鼠体内抑制ＣＯＣ２ｎ移植瘤的生长，体外和淋巴因子激活杀伤细胞（ＬＡＫ）、淋巴因子和抗ＣＤ３单抗激活的杀伤细胞（ＣＤ３ＡＫ）细胞进行比较，体内和ＣＤ３ＡＫ细胞进行比较。结果ＣＴＬ、ＣＤ３ＡＫ和ＬＡＫ对ＣＯＣ１细胞的细胞毒作用分别为７９．４％、５２．１％和５１．７％（Ｐ＜０．０１）。在体内，ＣＴＬ与ＣＤ３ＡＫ和未经处理的对照组比较，卵巢癌细胞移植到裸鼠体内的第９天，肿瘤平均体积分别为４４．４±２４．２ｍｍ３、１１８．８±４０．０ｍｍ３和４４３．０±１５８．７ｍｍ３（Ｐ＜０．０１），裸鼠平均生存期分别为２８．５天、２５．５天和１７天（Ｐ＜０．０１）。结论本方法诱导产生的ＣＴＬ，在体内、体外对卵巢癌细胞均有较高的细胞毒作用，能明显抑制肿瘤生长，为卵巢癌治疗提供了新的思路。     

妊娠肝内胆汁淤积症患者雌孕激素水平及免疫功能的变化

目的探讨雌孕激素水平及免疫功能变化与妊娠肝内胆汁淤积症（ＩＣＰ）的关系。方法采用放射免疫法、单向免疫扩散法、碱性磷酸酶及抗碱性磷酸酶法（ＡＰＡＡＰ）检测ＩＣＰ孕妇５０例（ＩＣＰ组）及正常妊娠妇女５０例（对照组）雌孕激素水平的变化、体液免疫和细胞免疫功能的水平。结果ＩＣＰ组雌激素水平（２５．８９±６．８５μｇ／Ｌ）较对照组（１６．９２±４．９８μｇ／Ｌ）明显升高,（Ｐ＜０．０１）,孕激素水平差异无显著性（Ｐ＞０．０５）;ＩＣＰ组细胞毒性抑制性Ｔ细胞（ＣＤ８＋）水平（１９．０６±１．９３％）较对照组（２６．４３±２．８９％）降低（Ｐ＜０．０５）,辅助性诱导性Ｔ细胞（ＣＤ４＋）与ＣＤ８＋比值（２．２３±０．３８）较对照组（１．７３±０．２３）升高（Ｐ＜０．０５）;雌激素水平与ＣＤ８＋呈负相关,与ＣＤ４＋／ＣＤ８＋比值呈正相关。结论ＩＣＰ患者雌激素水平增高,雌激素通过ＣＤ８＋上的雌激素受体而发挥作用,导致免疫功能的改变,而引起ＩＣＰ的发生     

卵巢功能早衰患者抗卵巢抗体及细胞免疫功能的测定

目的：探讨卵巢功能早衰（ＰＯＦ）患者细胞免疫功能的变化及其与抗卵巢抗体（ＡＯＡｂ）之间的关系。方法：检测３０例正常妇女（对照组）和３０例ＰＯＦ患者（ＰＯＦ组）的血清ＡＯＡｂ、外周血Ｔ淋巴细胞亚群及对卵巢抗原的白细胞促凝血活性（ＬＰＣＡ）。结果：对照组血清ＡＯＡｂ水平为１．３９±０．７２ｋＵ／Ｌ，ＰＯＦ组血清ＡＯＡｂ水平为６．８０±１．９１ｋＵ／Ｌ，两者比较，差异有极显著性（Ｐ＜０．０１）。与对照组比较，ＰＯＦ组ＣＤ＋３、ＣＤ＋４细胞百分率（分别为６５．４２±５．３１％和４４．７９±５．９０％）明显升高，ＣＤ＋８细胞百分率（２５．６３±４．２６％）明显降低，ＣＤ４＋／ＣＤ＋８比值（１．６６±０．２７）增加（Ｐ＜０．０１）。ＣＤ＋４／ＣＤ＋８比值升高者的ＡＯＡｂ阳性率（８５．７％，１８／２１），明显高于ＣＤ＋４／ＣＤ＋８比值正常者（３／９，Ｐ＜０．０１）。ＡＯＢｂ阳性的ＰＯＦ患者ＬＰＣＡ水平上升，且与ＡＯＡｂ之间有非常显著的相关性（χ２＝８．３７８，Ｐ＜０．０１）。结论：ＰＯＦ患者对卵巢抗原同时产生细胞免疫和体液免疫反应，ＰＯＦ的发病可能与免疫因素有关。     

野生型p53基因对人卵巢癌细胞株的生长抑制作用

目的：探讨野生型ｐ５３(ｗｔ ｐ５３)基因对人卵巢癌细胞株的生长抑制作用。方法：利用脂质体介导，将含有人全长ｗｔ－ｐ５３基因ｃＤＮＡ的真核表达载体质粒ｐＣＥＰ４／ｐ５３和空载体质粒ｐＣＥＰ４分别转染卵巢癌ＳＫＯＶ３细胞株，分别命名为ＳＫＯＶ３－ｐＣＥＰ４／ｐ５３细胞(C组)、ＳＫＯＶ３－ｐＣＥＰ４细胞(B组)，另设ＳＫＯＶ３细胞为正常对照组（Ａ组）。观察细胞体外生长情况和凋亡变化。结果：外源性ｐ５３基因在Ｃ组细胞中获表达，细胞生长曲线显示ｐ５３的导入使ＳＫＯＶ３细胞生长受到明显抑制，Ｃ组平均细胞集落数（１８．６±１．８个）少于Ａ组（２４．３±２．２个）和Ｂ组（２２．７±２．６），差异有显著性（Ｐ（005）。ＭＴＴ法检测C组细胞活力明显低于Ａ、Ｂ组。Ｃ组细胞Ｇ０～Ｇ１期百分比（５７．７９％）及凋亡细胞百分比（13．９1％）均高于Ｂ组（４６．０２％、２．０８％）和Ａ组（４３．６２％、０）。结论：ｗｔ－ｐ５３基因可介导ＳＫＯＶ３细胞Ｇ１期停滞和细胞凋亡，抑制细胞体外生长。     

肺表面活性物质的运用与新生儿肺透明膜病的转归

目的　探讨肺表面活性物质（Ｅｘｏｓｕｒｆ） 治疗新生儿肺透明膜病（ＨＭＤ） 的疗效及其并发症。　方法　采用回顾性分析的方法对用药前、用药后３０ 分钟及用药后６ 小时血气指标,机械通气参数进行比较分析,通过肺氧合情况判断疗效,分析转归及并发症。　结果　１６ 例患儿血气指标及机械通气的参数用药前与用药后６ 小时比较差异有显著性,氧分压（ＰＯ２） 由（４１－６ ±６ ．０）ｍ ｍ Ｈｇ（１ｍ ｍ Ｈｇ＝０ ．１３ ｋＰａ） 升高至（６２－９ ±２－０） ｍ ｍ Ｈｇ,动脉压泡氧分压比值（ａ／ＡＰＯ２） 由（０－１２ ±０－０３）ｍ ｍＨｇ 升高至（０－２４ ±０－１１）ｍ ｍ Ｈｇ,ＰＯ２／ＦｉＯ２ 由（７４－６ ±１６－４）ｍ ｍＨｇ 升高至（１４７－４ ±５９－９）ｍ ｍＨｇ,ＦｉＯ２ 由０－５８±０ ．０７ 降至０－４５ ±０ ．０９ ,ＭＡＰ由（１３－０ ±１．３）ｍ ｍ Ｈｇ 降至（１１－１ ±１－６）ｍｍ Ｈｇ。２４ 小时内复查胸片１３ 例明显好转,２ 例无改变,１ 例合并肺出血。其中痊愈１２ 例（治愈率７５ ％ ）,死亡２ 例,放弃２ 例。　结论　肺表面活性物质（Ｅｘｏｓｕｒｆ） 的运用可以改善肺透明膜病的转归,降低病死率     

氯菧酚胺对增生期子宫内膜组织学的影响及其与血清雌激素的关系

本文应用图像分析仪定量分析２１例不孕妇女氯菧酚胺（ＣＣ）用药前后周期第１２天子宫内膜组织结构，及其与血Ｅ２浓度的关系。结果表明：ＣＣ周期血Ｅ２显著高于自然周期同期水平，分别为１９５１．９２±１２４９．２８ｐｍｏｌ／Ｌ和４４９．６９±２５７．３６ｐｍｏｌ／Ｌ（Ｐ＜０．００１）；腺体面积小于自然周期值，分别为５２３５．５３±１００４．５２μｍ２和６４４４．６１±１２７３．２３μｍ２（Ｐ＜０．０５）。相关分析示自然周期血Ｅ２浓度与腺体面积呈正相关性（Ｐ＝０．００２），ＣＣ周期两者无相关性（Ｐ＞０．０５）。提示：ＣＣ阻断Ｅ２作用，抑制增生期子宫内膜腺体发育，但与高血Ｅ２不相关。     

CD56~(bright)CD16~-NK细胞CD49d、CD11b及孕激素在稽留流产患者外周血的表达及意义

目的:探讨稽留流产患者外周血CD56brightCD16-NK细胞中整合素α4(CD49d)和整合素αm(CD11b)及血清孕酮(P)的表达及意义。方法:采集50例早期正常妊娠及50例稽留流产孕妇外周血,流式细胞术检测外周血CD56brightCD16-NK细胞含量及CD56brightCD16-NK中CD49d、CD11b表达,酶联免疫吸附法检测血清P含量。结果:稽留流产组外周血CD56brightCD16-NK细胞的表达较早期正常妊娠组显著降低(P0.05);早期正常妊娠组的外周血CD56brightCD16-NK细胞上CD49d、CD11b的阳性表达率及血清P值均显著高于稽留流产组(P0.05)。稽留流产组患者的血清CD49、CD11b和孕激素水平有一定的相关性(r=0.346,P0.05)。结论:CD49d、CD11b及P可能在妊娠早期子宫自然杀伤(uNK)细胞的募集中起重要作用,同时也可能参与了正常妊娠的维持。     

Differential expression of CD40 and CD95 in ovarian carcinoma

PURPOSE: The role of CD95 (Fas) as a mediator of apoptosis has been well documented. CD40 ligation has been recently shown to initiate apoptosis and modulate CD95 mediated apoptosis in normal and some neoplastic tissues. Here we report the expression of CD95 and CD40 in cryopreserved cell suspensions from ovarian cancer associated ascites, fresh primary and recurrent ovarian carcinoma (OVCA) specimens, and ten established ovarian cancer cell lines. The effect of CD95 and CD40 receptor binding on apoptosis is described in two cell lines. EXPERIMENTAL DESIGN: Ascites specimens, fresh primary and recurrent OVCA specimens were dissociated to single cell suspensions. Expression of CD95 and CD40 was analyzed using flow cytometry. Apoptosis was determined via annexin uptake by flow cytometry following incubation with anti-CD95 antibody, CH11 and trimeric CD40L. RESULTS: Ascites showed the highest expression of both CD95 and CD40. Recurrent OVCA, in contrast, expressed low levels of CD95 and CD40. Primary OVCA showed moderate expression of both receptors. CD40 expression in ascites was significantly greater when compared to solid specimens (p < 0.05). Both CD40 and CD95 were strongly expressed in eight of ten cell lines studied. Binding of CD40L did not influence CD95 mediated apoptosis. CONCLUSIONS: CD40 is ubiquitously expressed in ovarian carcinomas and expression differs between ascites and solid tumor. There may be differential expression of both CD40 and CD95 in recurrent vs primary ovarian carcinoma, which may contribute to increased clinical malignancy of recurrent disease. In contrast to other epithelial malignancies, CD40 ligation does not appear to modulate CD95 mediated apoptosis.     

维吾尔族宫颈癌人乳头瘤病毒感染与CD4+CD25+CD127-调节性T细胞的相关性研究

目的:探讨维吾尔族宫颈癌人乳头瘤病毒(HPV)感染与CD4+CD25+CD127-调节性T细胞(Treg)的相关性.方法:采用HPV核酸扩增分型检测试剂盒及流式细胞术检测66例宫颈癌、35例宫颈上皮内瘤变(CIN)Ⅱ～Ⅲ、45例宫颈CIN Ⅰ维吾尔族患者和40例维吾尔族正常对照组HPV分型和外周血中T细胞亚群及CD4+...     

Expression of complement regulatory proteins-CD 35, CD 46, CD 55, and CD 59-in benign and malignant endometrial tissue

OBJECTIVE: Complement system plays an important role in host defense mechanisms against microorganisms and tumor cells. To protect themselves from autologous complement-mediated damage, normal host tissues express cell membrane-associated complement regulatory proteins (CRPs). To investigate whether neoplastic endometrial tissues overexpress these proteins to escape complement damage, we examined the distribution of complement receptor type 1 (CR1, CD35), membrane cofactor protein (MCP, CD46), decay-accelerating factor (DAF, CD55), and protectin (MACIF, CD59) on frozen endometrial tissue samples. METHODS: A total of 54 endometrial tissue samples were collected. Cryosections were obtained of 31 benign and 23 malignant tissue specimens. Tissue sections were stained by immunohistochemical staining procedure using specific antibodies and employing the avidin-biotin technique. Quantitation of the protein content of these CRPs was determined using the Samba 4000 image analysis system. RESULTS: For all four of the CRPs studied, a statistically significant difference in protein expression between the benign and malignant endometrial tissue specimens (P < 0.0001) was observed. CONCLUSIONS: Overexpression of all the CRPs studied (CD35, CD46, CD55, CD59) was observed in the malignant as compared with the benign endometrial tissues. The upregulation of these CRPs may promote resistance of the endometrial malignant tissue to complement-mediated damage, thereby allowing the tumor cells to escape from cytolysis and thus promoting carcinogenesis.     

Characterizing T-cell response in low-grade and high-grade vulval intraepithelial neoplasia, study of CD3, CD4 and CD8 expressions

OBJECTIVE: The objective of our study was to compare immunocyte infiltrates in vulval epithelium from low-grade and high-grade vulval intraepithelial neoplasia (VIN) lesions to determine if difference in T-cell presence reflected the grade of VIN. MATERIAL AND METHODS: Thirty-six vulval specimens were obtained from 24 patients who had previously undergone vulval biopsies for VIN, 14 high-grade diseases (VIN 3 with or without HPV) and 14 low-grade diseases (VIN 1 and VIN 2 with or without HPV). Eight samples of normal vulval tissue were selected from the excision margins of resected vulval biopsies. The lymphocyte surface markers included CD3 (Pan T-cell marker), CD4 (T helper cells), and CD8 (T cytotoxic cells). Each tissue section was visualized under high power magnification and cells were counted in 10 random areas at the dermo-epidermal junction. RESULTS: A significantly higher number of total mean T lymphocytes were detected in VIN specimens compared to normal vulval tissue (P = 0.002). In low-grade VIN, there were significantly more CD8 cells than CD4 when compared to high-grade VIN. This difference in CD4/CD8 ratio was significant (P = 0.001). CONCLUSIONS: This study suggests that increased CD8 response in VIN is a feature of low-grade disease and we speculate that this may be a protective mechanism. In high-grade disease, both CD4 cells and CD8 cells are equally present with preservation of normal CD4/CD8 ratio.     

CD26 and CD30 expression on the surface of lymphocyte during normal pregnancy

It is increasingly apparent that the Th1/Th2 cell ratio is decreased during pregnancy. In a previous study, we revealed that combined analysis of soluble CD26 and CD30 might be a potent surrogate tool for evaluating the Th1/Th2 balance during pregnancy. Therefore, in the present study, we elucidated whether the CD26 and CD30 expression on the surface of lymphocyte is useful marker for Th1 and Th2 lymphocytes, respectively, during pregnancy with flow cytometric technique. The peripheral blood samples were obtained from 6 non-pregnant healthy women, 8 healthy pregnant women in the first trimester and 12 pregnant women in the third trimester. The mean percentages of CD26 expression did not differ significantly among these groups (p = 0.45). Also, the mean percentages of CD30 expression did not differ significantly among these groups (p = 0.32). From the present study, the expression of CD26 and CD30 did not appear to be a useful marker for Th1 and Th2 lymphocytes during pregnancy.