高效液相色谱法测定草乌叶中新乌头碱、乌头碱和次乌头碱

目的 建立以高效液相色谱法同时测定蒙药草鸟叶中新乌头碱、乌头碱、次乌头碱的方法.方法 Extend-C18柱(250 mm×4.6 mm,5μm),流动相为甲醇-0.3 mol/L三乙胺(65:35),体积流量为0.8 mL/min,检测波长为235 nm,柱温为35℃.结果 经过方法学考察,本测定方法具有一定的专属性、准确性、重现性和可行性,新乌头碱、乌头碱、次乌头碱分别在1～5、0.35～1.75、0.5～2.5 μg呈良好的线性关系,平均加样回收率分别为98.43%、98.98%、98.82%,RSD分别为1.38%、0.89%、1.03%.结论 本方法简便、准确、分离效果好、线性范围宽、灵敏度高,可用于本品的质量控制.     

蒙药紫花高乌头根中二萜生物碱的分离与结构鉴定

目的进一步对蒙药紫花高乌头Aconitum excelsum Reichb．根的化学成分进行分离鉴定。方法通过硅胶柱层析分离，以IR，MS,^1H—NMR，^13C—NMR等光谱鉴定结构。结果从紫花高乌头根中分离得到6个化合物，经光谱分析和理化性质鉴定，确定其结构式分别是ranaconitine（Ⅰ），N—deacetyl lappaconitine（Ⅱ），1α，6，16，-三甲氧基-4，7，8，9，14α-五羟基-N-乙基乌头烷（Ⅲ），N—deacetylranaconitine（Ⅳ），β-谷甾醇（Ⅴ），豆甾醇（Ⅵ）。结论化合物Ⅱ，Ⅲ，Ⅳ，Ⅴ，Ⅵ为首次从该植物中得到，其中化合物Ⅲ为新二萜生物碱。     

藏药狭叶红景天化学成分研究(英文)

研究藏药狭叶红景天的化学成分,应用硅胶、ODS和Sephadex LH-20凝胶柱色谱以及反相HPLC等多种技术进行分离和纯化,应用波谱技术确定化合物的结构。从藏药狭叶红景天乙醇提取物中分离鉴定了11个化合物,其中4-羟基-苯乙基-(4′-甲氧基-苯乙基)醚(1)和β-D-葡萄糖苷-2-羟基-2-甲基-丁酸酯(2)为新化合物;对乙氧基苯乙醇乙酯(3),对羟基苯甲酸乙酯(5),对羟基苯甲醛(7),R(-)-mellein(8),对甲氧基苯乙醇(10)5个化合物为首次从红景天属植物中得到,对羟基苯乙酮(4),对羟基苯甲酸(6),豆甾醇(9),3,4,5-三羟基苯甲酸甲酯(11)4个化合物为首次从狭叶红景天中得到。     

附子地上部分化学成分研究

目的 研究附子地上部分的化学成分.方法 采用色谱法进行分离,根据理化性质和波谱数据鉴定化合物的结构.结果 分离得到5个化合物,分别鉴定为次乌头碱(1)、10-羟基乌头碱(2)、新乌头碱(3)、乌头原碱(4)和β-谷甾醇(5).结论 之前没有对附子地上部分进行化学成分研究的报道,本研究首次从中分离的到了化合物1～5.     

羊踯躅的化学成分研究

对羊踯躅植物花蕾的化学成分进行研究,应用多种色谱技术进行分离纯化、波谱技术鉴定其结构。从羊踯躅花蕾中分离并鉴定了15个化合物,分别为2E,4Z-脱落酸（1）,2α-羟基齐墩果酸（2）,齐墩果酸（3）,积雪草酸（4）,苯甲基糖苷（5）,邻苯二甲酸二丁酯（6）,β-谷甾醇（7）,牡荆素（8）,槲皮素（9）,硬脂酸（10）,rhodomollein Ⅰ（11）,rhodojaponin Ⅵ（12）,rhodomollein Ⅺ（13）,rhodojaponin Ⅱ（14）,kalmanol（15）。其中,化合物1 10为首次从羊踯躅植物中得到。     

Chemical constituents from Paliurusramosissimus

In the present research, in order to study the chemical constituents of Paliurusramosissimus(Lour.) Poir., the isolation of its ingredients was performed by repeated chromatography on silica gel, Sephadex LH-20, ODS and preparative HPLC. Their structures were elucidated on the basis of combination of mass spectrometry and1D,2DNMRspectroscopy. A total of 8 compounds were obtained, and their structures were identified as 3β-hydroxy-27-(3,4-dihydroxybenzoyl)oxylup-20(29)-en-28-oic acid(1),betulinic acid (2), lupeol(3), 27-O-trans-caffeoylcylicodiscic acid (4), ceanothic acid (5), 24-hydroxyceanothic acid (6), dihydrokaempferol (7), eriodictyol (8). Among them, compound 1 was a new compound. Compounds 3–4, 7–8 were isolated from Paliurus ramosissimus (Lour.) Poir. for the first time.     

Chemical constituents from the fruit calyx of Physalis alkekengi var. francheti

In the present study, in order to investigate the chemical constituents of Physalis alkekengi L. var. franchetii (Mast.) Makino, the isolation of ingredients was performed by repeated chromatography on silica gel, Sephadex LH-20 and preparative HPLC. Their structures were identified based on 1D, 2D NMR, and mass spectral analysis. A total of 14 compounds were obtained, and their structures were identified as physalin P (1), 4,7-didehydroneophysalin B (2), physalin D (3), 5α-hydroxy-25,27-dihydro-7-dehydro-7-deoxyneophysalin A(4), 4,7-didehydrophysalin B (5), ursolic acid (6), wogonin (7), blumenol A (8), nobiletin (9), liquiritigenin (10), schizandrin (11), 5-hydroxymethylfurfural (12), 5-(hydroxymethyl)-2-(dimethoxymethyl)furan (13), 1-O-[3-O-2-methyl-5-(2,3,4-trimethyl)phenyl-2,3-pentanediol]-β-D-xylopyranosyl-(1→6)-β-D-galactopyranoside(14). Among them, compound 14 is a new compound. Compounds 7–11, 13 are isolated from Physalis alkekengi L. var. franchetii (Mast.) Makino for the first time.     

Chemical constituents from the stem of Embelia laeta

In the present study, we aimed to investigate the chemical constituents from Embelia laeta. The constituents were separated by various column chromatography, including silica gel, ODS gel, MCI gel, Sephadex LH-20, and semipreparative HPLC. Their structures were elucidated by spectroscopic means. A total of 13 compounds were isolated from E. laeta and identified as (6R,9R)-1-Oxo-α-ionol-9-O-β-D-goucopyranoside (1), (+)-Pinoresinol (2), Leptolepisol D (3), Kaempferol (4), Kaempferol-3-α-L-Ramnopyranoside (5), Kaempferol-3-β-D-(6-O-trans-p-coumaroyl) glucopyranoside (6), Bis(2-ethylhexyl) phthalate (7), 2-Benzenedicarboxylic diisobutyl ester (8), Caffeic acid methyl ester (9), Paldehyde (10), Coniferaldehyde (11), Caffeic acid (12) and 5-Hydroxymethyl furfural (13), respectively. All the compounds were isolated from E. laeta for the first time.     

Chemical constituents from Croton crassifolius Geisel

Croton crassifolius Geisel belongs to the genus croton of the family euphorbiaceae. By means of solvent extraction and repeated chromatorgraphy on silica gel, Sephadex LH-20 and HPLC, the ethanol extract of the roots of Croton crassifolius Geisel was chemically investigated, which led to the isolation of nine diterpenoids. By using spectroscopic analysis, their structures were identified as follows: Crassifolin P (1), Crassifolin J (2), 1,5,6-trimethyl-5-(2-(5-oxo-2,5-dihydrofuran-3-yl)ethyl)-1,2,3,4,5,6,7,8-octahydronaphthalene-1-carboxylate (3), Penduliflaworosin (4), isoteucvin (5), Crassifolin G (6), teucvin (7), Chettaphanin I (8), mallotucin D (9). Among of them compound 1 was a new compound.     

王瓜化学成分研究（英文）

本文对葫芦科植物王瓜化学成分进行了研究,分离得到1个新化合物, 9个已知化合物。通过波谱解析、理化常数及比对文献数据资料进行结构鉴定,分别为3β-[(E)-caffeoyloxy]-D:C-friedooleana-7,9(11)-dien-29-oicacid(1),cucurbitacin B (2), 23,24-dihydrocucurbitacin B (3), 23,24-dihydrocu-curbitacin D (4), cucurbitacin D (5), cucurbalsaminol A (6),3-epi-isocucurbitacin D (7), curbitacin G (8), cucurbitacin J (9) and cucurbitacin I (10),所有化合物均首次从该植物中分离得到。     

Chemical constituents from Teucrium viscidum

A new monoterpenoid, 4,​5,​8-​trihydroxy-​6(7)-en-​decenoic acid γ-​lactone (1) and six known compounds, 3,7-dimethyloct-1-ene-3,6,7-triol (2), 2,8-bornanediol (3), 1-(4-hydroxyphenyl)ethane-1,2-diol (4),2-(3-methoxy-4-hydroxyphenyl)-propane-1,3-diol(5), indole-3-carboxylic acid (6), and 3-hydroxy-benzenemethanol (7) were isolated from the whole parts of Teucrium viscidum. Their structures were established by a combination of spectroscopic data analysis, besides comparison with literature data. Compounds 2–7 described above were isolated from this genus for the first time.     

Two new diterpenoid alkaloids, beiwusines A and B, from Aconitum kusnezoffii

Two new atisine-type diterpenoid alkaloids, beiwusine A (1) and B (2), have been isolated from the roots of Aconitum kusnezoffii Reichb. Their structures were established on the basis of spectroscopic data. Beiwusines A and B are the first examples of atisine-type diterpenoid alkaloids having a hydroxyl group at C-1. In addition, one known diterpenoid alkaloid spiramine H (3) has been isolated.     

Chemical constituents isolated from the endophytic fungus Penicillium commune

Phytochemical investigation of the endophytic fungus Penicillium communeled to the isolation of nine known compounds.Based on the spectroscopic and chemical properties, their structures were identified as 6-acetyl-2α,5-dihydroxy-2-(2-hydroxypropyl)-3α,8-dimethylchroman (1), communol B (2), communol E (3), communol G (4), 2-hydroxy-4-phenylquinoline (5), viridicatol (6), 3-O-methylviridicatin (7), trans-ferulic acid (8), 1,2-benzenediol,4-(2-methoxyethenyl) (9). Compounds 1 and 5-9 were isolated from this fungus for the first time.     

制马钱子的化学成分研究(英文)

从制马钱子中分离并鉴定了15个化合物,分别为番木鳖碱(1),马钱子碱(2),伪番木鳖碱(3),伪马钱子碱(4),裂环马钱素酸(5),咖啡酸(6),对羟基苯甲酸(7),对羟基苯乙酸(8),熊果醇(9),7,22,25-豆甾三烯-3-醇(10),羽扇豆醇(11),11-酮基-香树脂棕榈酸酯(12),儿茶酚(13),麦芽酚(14),腺苷(15)。化合物5-15为首次从马钱属植物中分离得到。     

西沙海绵Agelas aff .nemoechinata化学成分研究△

目的 研究西沙海绵Agelas aff .nemoechinata的化学成分。方法 利用Sephadex LH-20凝胶色谱、硅胶色谱、ODS色谱、HPLC等手段对化学成分进行了分离纯化;通过理化性质,波谱分析方法结合文献对照,鉴定化合物的结构。结果 从南海海绵甲醇溶剂提取物中,分离鉴定了8个单体化合物：尿嘧啶(1)、胸腺嘧啶(2)、1-H-吡啶酮(3)、胸腺嘧啶-2’-脱氧核苷(4)、2-甲酰胺吡咯(5)、3-醛基吲哚(6)、6-羟基-4,4,7a-三甲基-5,6,7,7a-四氢苯并呋喃-2(2H)酮(7)、5-氨基戊酸(8)。结论 化合物1~8均为首次从该种海绵中分离得到。化合物7 在50 μg/mL_-1浓度水平,对K562白血病细胞株、P388白血病细胞株和HeLa肿瘤细胞株的抑制率分别是38.29%、23.23%和95.57%。     

云芝的化学成分

目的研究云芝的化学成分。方法用色谱学方法进行分离纯化,以波谱学方法进行结构解析。结果从云芝中分离并鉴定了八个化合物,分别为： 麦角甾-7,22-二烯-3β-棕榈酸酯（1）,麦角甾-7,22-二烯-3β-醇（2）,白桦脂酸（3）,4-羟基苯甲酸（4）,3-甲氧基-4-羟基苯甲酸（5）,3,5-二甲氧基-4-羟基苯甲酸（6）,2-呋喃酸（7）和烟酸（8）。结论八个化合物均为首次从云芝中分离得到。     

Chemical constituents from the leaves of Premna microphylla Turcz

Phytochemical investigation of the leaves of Premna microphylla Turcz led to the isolation of 13 known compounds. Based on spectroscopic and chemical evidences, their structures were identified as diosmetin (1), blumenol A (2), (3S,5R,6S,7E,9R)-5,6-epoxy-3,9-dihydroxy-7-megastigmene (3), 3β-hydroxy-5α,6α-epoxy-7-megastigmen-9-one (4), ixerol B (5), (-)-dehydrovomifoliol (6), 3S,5R-dihydroxy-6S,7-megastigmadien-9-one (7), loliotide (8), (+)-dehydrololiolide (9), (+)-medioresinol (10), 4-oxopinoresinol (11), tormentic acid (12), and indole-3-carboxylic acid (13). Compounds 2–13 described above were isolated from this genus for the first time.     

川乌氯仿部位的化学成分研究

目的研究川乌氯仿部位的化学成分。方法通过硅胶、碱性氧化铝柱色谱进行分离纯化,并根据理化性质和波谱数据鉴定化合物的结构。结果分离得到9个化合物,分别鉴定为次乌头碱(1)、金色酰胺醇酯(2)、乌头碱(3)、松胞素B2(4)、宋果灵(5)、新乌头碱(6)、多根乌头碱(7)、北乌碱(8)、附子灵(9)。结论化合物2为首次从乌头属植物中分离得到,化合物4为首次从植物界中分离得到。     

卵叶远志根的化学成分研究

本文对卵叶远志Polygala sibirica L．根的化学成分进行研究。应用硅胶、Sephadex LH-20、ODS柱以及半制备液相色谱进行分离和纯化，应用波谱技术确定化合物的结构。从卵叶远志根中分离鉴定了12个化合物，分别是：tenuifoliside A（1），tenuifoliside B（2），glomeratose A（3），3,6．二-O-芥子酰基蔗糖（4），sibiricose A5（5），sibiricose A6（6），sibiricose A1（7），sibiricoseA2（8），polygalatenoside E（9），1-O-L-吡喃阿拉伯糖基-D-（6→1）-β-D-吡喃葡萄糖基·水杨酸甲酯（10），eanthosideA（11），3,4，5-三甲氧基肉桂酸甲酯（12）。化合物11首次从远志属中分离得到，化合物2，9，10和12均为首次从该植物中分离得到。     

万寿菊花的化学成分

目的 研究万寿菊Tagetes erecta L.花中的化学成分.方法 95%乙醇提取制备总浸膏,硅胶柱层析分离其成分,波谱法鉴定其结构.结果 分得的10个已知化合物分别为dammarenediolⅡ3-O-n-palmitate (1)、乌发醇(2)、槲皮万寿菊素3-O-葡萄糖苷(3)、quercetagetin 5-methyl ether (4)、5,7-dimethoxy quercetin(5)、没食子酸(6)、3,4-二羟基-5-甲氧基苯甲酸(7)、16Z,19Z-pentacosadienoic acid(8)、亚油酸甘油单酯(9)和Vit Eα (10).结论 10个化合物中,1、2、6、7、8、9、10为首次从该植物中分离得到.