我国医学期刊近两年发表的眼底病研究论文情况分析

目的 分析我国医学期刊眼底病研究论文的刊出情况,为我国眼底病的研究和发展提供参考依据。数据来源 收集1999年1月至2001年6月,我国418种医学期刊（416种为中文期刊,2种为英文)公开发表的眼底病临床与基础研究论文,并进行文献计量学分析。数据选择 我国418种医学期刊发表的有关眼底病研究文献2352篇,其中论著1651篇,病例报告324篇,综述171篇。论著中,基础研究402篇,临床研究530篇,中医及中西医结合治疗性论著270篇,检测技术的临床应用295篇,流行病学研究142篇,其他研究12篇。结果 分析结果显示视网膜脱离、眼外伤、视网膜静脉阻塞、糖尿病性视网膜病变等是目前我国眼底病研究的重点;眼科期刊是刊载眼底病研究成果的主要刊物,但也有相当比例的眼底病研究文章发表于其他综合性医学期刊上。目前尚缺乏有关我国眼底病研究资源及其应用情况的论述,缺乏对临床医疗措施有效性的系统评价论述。结论 我国眼底病的临床及基础研究工作已具有相当规模,是一个较为活跃的研究领域。但我国对眼底病研究资源的评估和利用,对临床医疗措施的系统分析和评价尚需加强。     

Trends in ophthalmology journals: a five-year bibliometric analysis (2009-2013)

AIM: To explore the trends in the ophthalmic literature over a 5-year period in relation to country, research expenditure and demographics. METHODS: Articles published between 2009 and 2013 by the 20highest-contributing countries in the 20 top-ranked ophthalmology journals were identified by their country of affiliation. The number of articles published and mean impact factor were measured per country for each year and trends explored using regression analysis with 5-year and 10-year forecasts calculated. Data on research expenditure was collected and tested for correlation with the number of articles and mean impact factor. RESULTS: The analysis included 19 338 articles. The USA, UK and Europe accounted for 60.2% of articles published, with the USA contributing 7388 articles (34.0%). The USA also demonstrated the highest mean impact factor (3.5). Research expenditure was significantly correlated with both research output (r=0.86, P<0.001) and scholarly impact (r=0.42, P<0.001). China (P<0.01), Korea (P<0.01) and India (P<0.02) demonstrated a significant growth in research output over the study period. The research contribution of these three countries combined is forecasted to overtake that of Europe within ten years, with China expected to be the second-largest contributor within five years. These countries were also among those demonstrating the greatest growth in research expenditure. CONCLUSION: While the USA and European countries are major contributors of ophthalmic research, the productivity of some Asian countries is growing impressively. The contribution of China, Korea and India is forecasted to outweigh that of Europe by 2023. Research expenditure is highly correlated with research productivity and these trends reflect the differing economic priorities across the world.     

血管内皮生长因子-C在喉癌中的表达

目的研究血管内皮生长因子-C(vascular endothelial growth factor,VEGF-C)在喉癌组织中的表达,探讨其在喉癌淋巴结转移中的作用.方法采用免疫组化方法,对40例喉癌组织蜡块标本及5例正常组织中的VEGF-C表达进行检测.结果40例喉癌组织标本中有28例呈阳性表达,且和淋巴结转移有关.正常组织呈弱阳性或无表达.结论喉癌组织中VEGF-C的表达与淋巴结转移及肿瘤转移有关.     

Human Hepatocyte Growth Factor (HGF) in the aqueous humor

Using an enzyme-linked immunosorbent assay, we measured the concentration of hepatocyte growth factor (HGF) in human aqueous humor and serum from 36 eyes of 33 patients and analyzed the relationship between HGF and several parameters of corneal endothelial cells. Aqueous HGF concentrations ranged from 0.020-0.194 ng/mL (average: 0.101 ± 0.044 ng/mL) and was correlated positively with corneal endothelial cell density (r = 0.343, P = 0.04). The aqueous HGF level did not correlate with other corneal endothelial cell parameters or the serum HGF level. The HGF receptor, c-Met, was found in the corneal endothelial cell membranes. This study suggests that the aqueous humor HGF is a factor which maintains an integrity of corneal endothelial cells.     

Acute thyroid eye disease (TED): Principles of medical and surgical management

The active inflammatory phase of thyroid eye disease (TED) is mediated by the innate immune system, and management is aimed at aborting this self-limited period of autoimmune activity. In most patients with TED, ocular and adnexal changes are mild and management involves controlling thyroid dysfunction, cessation of smoking, and addressing ocular surface inflammation and exposure. In patients with acute moderate disease, this being sufficient to impair orbital functions, immunosuppression reduces the long-term sequelae of acute inflammation, and adjunctive fractionated low-dose orbital radiotherapy is used as a steroid-sparing measure. Elective surgery is often required following moderate TED, be it for proptosis, diplopia, lid retraction, or to debulk the eyelid, and this should be delayed until the disease is quiescent, with the patient stable and weaned off all immunosuppression. Thus, surgical intervention during the active phase of moderate disease is rarely indicated, although clinical experience suggests that, where there is significant orbital congestion, early orbital decompression can limit progression to more severe disease. Acute severe TED poses a major risk of irreversible loss of vision due to marked exposure keratopathy, ‘hydraulic'' orbital congestion, or compressive optic neuropathy. If performed promptly, retractor recession with or without a suture tarsorrhaphy protects the ocular surface from severe exposure and, in patients not responding to high-dose corticosteroid treatment, decompression of the deep medial orbital wall and floor can rapidly relieve compressive optic neuropathy, as well as alleviate the inflammatory and congestive features of raised orbital pressure.     

u-PA和TGF-β1 在喉癌中的表达及临床意义

目的探讨u-PA和TGF-β1在喉癌中的表达及其与喉癌生物学行为之间的关系.方法采用SABC免疫组化染色方法检测了84例喉癌与10例正常喉组织中的u-PA和TGF-β1的表达情况.结果在喉癌组织中u-PA的表达水平与临床分期和有无淋巴结转移的相关性有统计学意义(P＜0.05),与组织学分型和发生部位的相关性无统计学意义(P＞0.05).在大部分喉癌组织中(89.29%)TGF-β1表达较高,与临床分期和有无淋巴结转移的相关性有统计学意义(P＜0.05),但与组织学分型和发生部位的相关性无统计学意义(P＞0.05).此外,u-PA和TGF-β1在喉癌中的表达和在正常喉黏膜中的表达差异均有统计学意义(P＜0.05).结论 u-PA和TGF-β1与喉癌生物学行为关系密切,它们的异常表达可能是喉癌发生、发展过程中的一个重要环节.