海通片对高脂血症大鼠血脂的调节作用

①目的观察海通片对高脂血症大鼠血脂的调节作用。②方法观察海通片预防性用药（３０ｄ）和治疗性用药（１５ｄ）对外原性高脂血症大鼠血清总胆固醇（ＴＣ）、甘油三酯（ＴＧ）、低密度脂蛋白（ＬＤＬ）和高密度脂蛋白（ＨＤＬ）含量的影响。③结果海通片预防性用药可使大鼠ＨＤＬ升高（ｔ＝２．２４１～２．７６６，Ｐ均＜０．０５），治疗性用药可使升高的ＴＣ迅速降至正常水平（ｔ＝２．３７０～３．０８７，Ｐ＜０．０５，０．０１），并使血清ＴＧ和ＬＤＬ含量显著降低（ｔ＝２．２９６～４．１２４，Ｐ＜０．０５，０．０１）。④结论海通片对高脂血症大鼠有调节血脂作用。     

绝经后应用小剂量利维爱妇女血浆蛋白、血糖和血脂的变化

目的：观察绝经后应用小剂量利缩爱妇女血浆蛋白、血糖和血脂的变化。方法：检测６０例妇女用药３,６,１２和２４个月时的血浆白蛋白（Ａ）、球蛋白（Ｇ）、血糖（ＧＬ）、总胆固醇（ＴＣ）、甘油三脂（ＴＧ）、高密度脂蛋白（ＨＤＬ）和低密度脂蛋白（ＬＤＬ）,结果：用药６个月时Ａ升高,以１２个月时升高最明显（Ｐ〈０．０１）。用药３个月后ＴＧ显著降低（Ｐ〈０．０５～０．０１）,ＨＤＬ用药３个月时有所降低（Ｐ〉０．０１）,以后渐恢复至用药前水平。用药后Ｇ、ＧＬ、ＴＣ、ＬＤＬ无明显变化,但ＧＬ、ＴＣ降低的妇女多于升高者。结论：小剂量利维爱可使绝经后妇女Ａ升高,明显降低ＴＧ,对Ｇ、ＧＬ、ＴＣ、ＨＤＬ、ＬＤＬ无明显影响。     

内源性高甘油三酯血症患者血浆高密度脂蛋白亚类分析

内源性高甘油三酯血症（ＨＴＧ）常伴血浆ＨＤＬ－Ｃ含量下降及ＨＤＬ组成异常。为了研究ＨＴＧ患者血浆ＨＤＬ亚类的改变,作者建立了密度梯度聚丙烯酰胺梯度凝胶电泳法（ＧＧＥ）,对２５例ＨＴＧ患者（血浆ＴＧ＞２．２６ｍｍｏｌ／Ｌ,ＴＣ＜６．７２ｍｍｏｌ／Ｌ）及２５例年龄、性别匹配的血脂正常者（血浆ＴＧ＜１．６９ｍｍｏｌ／Ｌ,ＴＣ＜５．１７ｍｍｏｌ／Ｌ）的ＨＤＬ亚类——ＨＤＬ２ａ、ＨＤＬ２ｂ、ＨＤＬ３ａ、ＨＤＬ３ｂ和ＨＤＬ３ｃ进行了分析,同时对血浆及ＨＤＬ中脂质及载脂蛋白（ａｐｏ）ＡⅠ、ＡⅡ、Ｂ１００、ＣⅡ、ＣⅢ及Ｅ进行了测定。结果发现,ＨＴＧ患者空腹血浆ＴＧ、ａｐｏＢ１００、ａｐｏＣⅡ、ａｐｃＣⅢ及ａｐｏＥ显著增加（Ｐ＜０．００１）,ＨＤＬ－Ｃ及ａｐｏＡⅠ显著降低（Ｐ＜０．００１及Ｐ＜０．０５）;患者血浆ＨＤＬ组成异常,ＨＤＬ中ＴＧ含量显著增加（Ｐ＜０．００１）,ＴＣ、ａｐｏＡⅠ、ａｐｏＣⅡ、ａｐｏＣⅢ及ａｐｏＥ含量下降（Ｐ＜０．０１）;患者血浆ＨＤＬ亚类含量改变,ＨＤＬ２ｂ显著降低（Ｐ＜０．０２）,ＨＤＬ２ａ及ＨＤＬ３ａ增加（Ｐ＜０．０５）,且随血浆ＴＧ含量的升高而升高,ＨＤＬ３ｂ及ＨＤＬ３ｃ则未见改变     

桑叶茶对高脂血症大鼠脂代谢的影响及其抗氧化作用

目的：观察桑叶茶对高脂血症大鼠的影响。方法：设正常对照组、高血脂对照组、高脂饲料对照组、阳性对照组。以上４组分别给普通饲料、高血脂饲料、高血脂饲料桑叶茶、高血脂饲料山楂精降脂片。４周后检测。结果：桑叶茶使高脂血症大鼠血清ＨＤＬ－Ｃ、ＨＤＬ－Ｃ／ＴＣ明显升高（Ｐ〈０．０５）,ＴＣ、ＬＤＬ－Ｃ、ＴＧ明显降低（Ｐ〈０．０５）,ＬＰＯ显著降低（Ｐ〈０．０１）,结论：桑叶茶可以降低血脂,抑制有害的过氧化物的     

肝舒乐保肝作用的实验研究

肝舒乐（ Ｇ Ｓ Ｌ）（２０ ,１０ ｇｋｇ ,ｐｏ） 对四氯化碳（ Ｃ Ｃｌ４ ） 及 Ｄ氨基半乳糖（ Ｄ Ｇａｌ） 所引起的小鼠血清谷丙转氨酶（ Ａ Ｌ Ｔ） ,谷草转氨酶（ Ａ Ｓ Ｔ） 活性升高有明显的降低作用（ Ｐ＜ ０ ．０１） ,并能明显提高小鼠网状内皮系统的吞噬功能及小鼠血清半数溶血值（ Ｐ＜ ０ ．０１） 。 Ｇ Ｓ Ｌ（１４ ,７ ｇｋｇ ,ｐｏ） 对 Ｃ Ｃｌ４ 引起的慢性肝损伤大鼠血清 Ａ Ｌ Ｔ、 Ａ Ｓ Ｔ 活性的升高有明显的降低作用（ Ｐ＜ ０ ．０１） 。     

血脂康和多烯康对高脂血症调脂作用的比较

采用血脂康与多烯康分别治疗高脂血症,血脂康组总有效率达９３．８％,用药４周ＴＣ、ＴＧ、ＬＤＬ－Ｃ、动脉粥样硬化指数（ＡＳ指数）均有显著下降（Ｐ〈０．０１）,用药８周进一步下降（Ｐ〈０．０１）,ＨＤＬ－Ｃ明显升高（Ｐ〈０．０５）。多烯康组用药８周ＴＣ、ＴＧ、ＬＤＬ－Ｃ、ＡＳ指数亦有明显下降,但ＨＤＬ－Ｃ无显著变化。结论：血脂康调脂作用明显优于多烯康。     

糖尿病患者血清糖化蛋白及高密度脂蛋白胆固醇水平测定及关系分析

本文检测了７８例糖尿病患者血清中糖化总蛋白（ＧＴＰ）和糖化白蛋白（ＧＡ）及其脂类水平。结果显示：糖尿病患者ＧＴＰ、ＧＡ水平显著高于对照组（Ｐ〈０．０１）；而ＨＤＬ－Ｃ及ＨＤＬ２－Ｃ水平显著降低（Ｐ〈０．０５）；ＧＴＰ、ＧＡ与ＨＤＬ－Ｃ间显示较好的负相关。提示：糖尿病患者血清ＡＴＰ、ＧＡ及ＨＤＬ－Ｃ及亚分测定，是对其血糖水平监视及动脉硬化预防的良好指标。     

四胺胶囊治疗胰岛素抵抗综合征的临床研究

中药复方制剂四安胶囊,对中医辨证属肝火亢盛,痰瘀互结的胰岛素抵抗综合征患者,能显著改善证候,总有效率９３．９％,证候积分由治疗前的２４．３±４．１降至１１．３±３．０（Ｐ〈０．０１）;降低收缩压及舒张压（Ｐ〈０．０１）,但与对照组相比无显著性差异（Ｐ〉０．０５）,降低血糖（Ｐ〈０．０１）,与对照组相比显著性显著（Ｐ〈０．０５）;调节血脂比例,使ＴＧ,ＴＣＨ,ＬＤＬ血脂指数及ＡＰＯＢ显著降低（Ｐ〈０     

普萘洛尔和恬尔心对脂蛋白代谢的影响

用体外培养人脐静脉内皮细胞单层（ＥＣ）观察普奈洛尔，恬尔心脏脂蛋白代谢的影响，将等量的高脂蛋白血清分别与两药混合后加于内皮细胞单层培养基上孵化１０ｈ，取培养上清液检测甘油三酯（ＴＧ），总胆固醇（ＴＣ），高密度脂蛋白（ＨＤＬ），低密度脂蛋白（ＬＤＬ），极低密度脂蛋白（ＶＬＤＬ），结果显示，普萘洛尔显著降低ＬＤＬ水平（Ｐ〈０．０１），对脂蛋白代谢产生的有益影响，恬尔心显著降低ＴＧ水平（Ｐ〈０．０５），     

成都地区正常男性中老年人血清apo B100含量与脂质及apoAⅠ、AⅡ、CⅡ、CⅢ、E的关系

为研究血清ａｐｏＢ１００与血清ＴＧ、ＴＣ、ＨＤＬ－Ｃ、ＬＤＬ－Ｃ及ａｐｏＡⅠ、ＡⅡ、ＣⅡ、ＣⅢ和Ｅ的关系，对４３８例４０～７０岁正常男性中老年人空腹１２～１４小时的血清ＴＧ、ＴＣ、ＨＤＬ－Ｃ、ＬＤＬ－Ｃ及ａｐｏＢ１００、ＡⅠ、ＡⅡ、ＣⅡ、ＣⅢ和Ｅ的水平进行了全面的分析。结果表明，血清ａｐｏＢ１００水平为７４２．５±１６５．８ｍｇ／Ｌ，且有随年龄上升的趋势。其中５组及６组的ａｐｏＢ１００水平比１、２、３、４组均显著升高（Ｐ＜０．０５）。血清脂质水平（ｘ±ｓ，ｍｍｏｌ／Ｌ）：ＴＧ为１．３９±０．４５、ＴＣ为４．７９±０．８２、ＨＤＬ－Ｃ为１．６２±０．４０、ＬＤＬ－Ｃ为２．６５±０．８１，且５组及６组的ＴＧ、ＴＣ、ＬＤＬ－Ｃ水平均比１、２、３、４组显著升高（Ｐ＜０．０１）。相关分析表明：血清ａｐｏＢ１００与血清ＴＧ、ＴＣ、ＬＤＬ－Ｃ及ａｐｏＣⅡ、ＣⅢ和Ｅ均呈显著正相关（Ｐ＜０．００１）；与ＨＤＬ－Ｃ呈显著负相关（Ｐ＜０．０１）。以上结果提示：中老年男性，尤其是６０～７０岁年龄段的血清ａｐｏＢ１００水平与血清脂质及ａｐｏＣⅡ、ＣⅢ和Ｅ有密切的关系。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.