鸢尾苷元对氧化损伤血管内皮细胞MCP-1和ICAM-1表达的影响

目的:观察鸢尾苷元对氧化低密度脂蛋白(ox-LDL)致氧化损伤血管内皮细胞(VEC)的保护作用及对MCP-1和ICAM-1mRNA表达的影响,探讨其抗动脉粥样硬化的作用机制。方法:采用体外培养大鼠VEC,加入ox-LDL建立VEC氧化损伤模型,加入不同剂量的鸢尾苷元,通过MTT比色法观察细胞活力,并采用RT-PCR方法检测VECMCP-1和ICAM-1mRNA的表达情况。结果:鸢尾苷元对ox-LDL所致的VEC损伤具有明显的保护作用,并显著抑制ox-LDL诱导VECMCP-1和ICAM-1mRNA的过度表达。结论:鸢尾苷元抗VEC氧化损伤和抑制MCP-1,ICAM-1的过度表达是其抗动脉粥样硬化的重要作用机制。     

牛磺酸通过p38通路对缺氧内皮细胞ICAM-1,VCAM-1表达的影响

探讨牛磺酸(taurine,Tau)通过p-p38通路对牛肺主动脉内皮细胞(PAECs)中ICAM-1,VCAM-1的影响及其作用机制。原代培养PAECs取4~12代细胞用于实验。分为5组:对照(control)组、缺氧(hyp)组、抑制剂(SB203580)组、给药(Tau)组、抑制剂+给药(SB+Tau)组,Tau的给药浓度为100 mmol·L~(-1),p38抑制剂SB203580浓度为20μmol·L~(-1),给药时间为12h。MTT检测不同浓度Tau对PAECs的抑制。使用Western blot及Real-time PCR方法检测p38通路蛋白及炎症因子ICAM-1,VCAM-1的表达情况。使用免疫荧光检测p38核位移情况。MTT结果显示随着Tau浓度增加,对PAECs增殖抑制增强。Western blot和Real-time PCR结果显示在蛋白水平及基因水平Tau都抑制ICAM-1,VCAM-1的表达。Western blot的结果和免疫荧光的结果均显示Tau可以抑制p38蛋白的活化。Tau可能通过p38 MAPK通路抑制由缺氧引起的牛肺主动脉内皮细胞中ICAM-1,VCAM-1的表达。     

丹酚酸A通过调节氧化应激抑制LPS诱导的人脐静脉血管内皮细胞NLRP3炎性小体的表达

目的 探讨丹酚酸A(salvianolic acid A,Sal A)对脂多糖(lipopolysaccharide,LPS)诱导人脐静脉血管内皮细胞(human umbilical vein endothelial cells,HUVECs)氧化应激及炎症的抑制作用.方法 脂多糖建立细胞损伤模型后,将细胞分为对照组、...     

丹酚酸A对缺氧/复氧损伤条件下脑微血管内皮细胞ICAM-1表达的影响

[目的]通过对观察丹酚酸A对缺氧/复氧(H/R)损伤条件下脑微血管内皮细胞细胞间黏附因子-1(CAM-1)表达的影响,探讨丹酚酸A对缺血再灌注急性期脑保护作用机制.[方法]培养BMEC,丹酚酸A预处理20 h后制作(H/R)/模型,逆转录-聚合酶链式反应(RT-PCR)法检测BMEC细胞间黏附因子-1 ICAM-1 mRNA的表达.以尼莫地平作为阳性对照药.[结果]模型组与空白对照组相比ICAM-1 mRNA表达明显升高(P<0.01).丹酚酸A组ICAM-1 mRNA表达低于模型组(P<0.05).[结论]丹酚酸A可降低H/R损伤条件下BMEC ICAM-1 mRNA表达的影响,提示阻抑粒细胞黏附作用是其脑保护作用机制之一.     

当归对单侧输尿管梗阻大鼠肾组织中黏附分子ICAM-1表达的影响

目的:观察当归对单侧输尿管梗阻(UUO)大鼠肾小管间质纤维化过程中黏附分子P-选择素(P-selectin)和细胞间黏附分子-1(ICAM-1)、转化生长因子-β1(TGF-β1)等细胞因子的表达和CD68单核/巨噬细胞浸润的影响。方法:采用大鼠模型,52只SD大鼠分为:假手术组、UUO组、当归组。术后第7d和第14d留取标本处死大鼠,用免疫组化方法检测肾组织内P-selectin、ICAM-1、CD68、TGF-β1的表达。结果:与假手术组相比,UUO组大鼠第7d和第14d肾组织内ICAM-1、P-selectin、CD68、TGF-β1的表达均显著上调(P<0.05)。与UUO组相比,当归组两个时间段肾小管间质病变减轻,且肾组织内ICAM-1、CD68、TGF-β1的表达受到显著抑制(P<0.05)。结论:当归可能是通过抑制ICAM-1的表达和炎细胞的组织浸润,从而降低了肾小管间质TGF-β1的水平和纤维化的进展。     

Effect of anemonin on NO, ET-1 and ICAM-1 production in rat intestinal microvascular endothelial cells

Anemonin (the dilactone of cyclobutane-1, 2-diol-1, 2-diacrylic acid) was isolated from the root of Pulsatilla chinensis Regel. Pulsatilla chinensis Regel has been used in the treatment of enteritis in China for years. However, only little was known about the mechanism underlying its anti-inflammatory effects. We investigated the effect of anemonin on the release of nitric oxide (NO), endothelin-1 (ET-1) and soluble intercellular adhesion molecule-1 (sICAM-1) induced by lipopolysaccharide (LPS) in primary cultures of rat intestinal microvascular endothelial cells (RIMECs). RIMECs were challenged with 1 microg/ml LPS with or without the presence of various concentrations of anemonin (1, 5 and 10 microg/ml). Anemonin significantly inhibited the production of NO and ET-1 induced by LPS at a concentration of 5 microg/ml and at 10 microg/ml anemonin down-regulated LPS-induced sICAM-1 expression. Anemonin itself had no effect on either factor. These findings suggest that anemonin may exert some beneficial therapeutic action in intestinal inflammation, at least in part by inhibiting the production of NO, ET-1 and ICAM-1 in RIMECs and thus preventing intestinal microvascular dysfunction.     

土茯苓抑制白介素-1诱导的血管内皮细胞细胞间黏附分子-1的表达

目的:探讨中药土茯苓对白介素-1(IL-1)诱导的血管内皮细胞表面的细胞间黏附分子-1(ICAM-1)表达升高的保护作用.方法:以原代培养的人脐静脉内皮细胞(HU-VEC)为研究对象,采用细胞培养、免疫荧光染色、中药血清药理学方法、流式细胞仪等技术,观察IL-1对培养的HUVEC ICAM-1表达的影响并研究不同时间点及不同浓度的含药血清的抑制作用.结果:IL-1使HUVEC的ICAM-1的表达明显升高,同时含药血清组的ICAM-1的表达比空白血清组低.结论:土茯苓含药血清能拮抗IL-1致内皮细胞ICAM-1表达升高.土茯苓的作用部分是多种成分作用于多个靶点的综合效应.     

广西匙羹藤紫外可见光谱鉴别的研究

目的:为匙羹藤的鉴别提供科学依据。方法:采用紫外-可见光谱法。结果:匙羹藤茎与叶各提取液的光谱特征吸收峰位大致相同。结论:可为匙羹藤的鉴别提供辅助手段。     

黄芩甙对重症急性胰腺炎大鼠胰腺ICAM-1和P-选择素表达的影响

目的观察黄芩甙对重症急性胰腺炎(SAP)大鼠胰腺组织ICAM-1和P-选择素表达的影响情况。方法将45只大鼠随机分为假手术组、模型组、黄芩甙组,每组15只。假手术组进腹后仅翻动胰腺和十二指肠后关腹,模型组、黄芩甙组在造模成功后分别给予生理盐水和黄芩甙。术后3,6,12 h,观察大鼠胰腺的病理变化,测定胰腺组织中髓过氧化物(MPO)和肿瘤坏死因子-α(TNF-α)的含量,用免疫组化方法检测胰腺组织ICAM-1和P-选择素的表达情况。结果术后3,6,12 h,模型组和黄芩甙组的病理评分均显著高于假手术组(P均<0.05),且模型组和黄芩甙组6,12 h的病理评分均显著高于3 h(P均<0.05),但2组评分在6 h和12 h间无显著性差异;术后3,6,12 h,模型组胰腺组织中MPO和TNF-α较假手术组显著降低(P均<0.05),黄芩甙组胰腺组织中MPO和TNF-α较模型组显著降低(P均<0.05);术后各时间点,模型组ICAM-1和P-选择素随时间延长显著增多,黄芩甙组则随时间延长而显著减少。结论黄芩甙可能通过抑制中性粒细胞的浸润,减轻ICAM-1和P-选择素的表达而发挥对胰腺组织的保护作用。     

CML-1 inhibits TNF-alpha-induced NF-kappaB activation and adhesion molecule expression in endothelial cells through inhibition of IkBalpha kinase

CML-1 is a purified extract from a mixture of 13 oriental herbs (Achyranthis Radix, Angelicae Gigantis Radix, Cinnamomi Cortex Spissus, Eucommiae Cortex, Glycyrrhizae Radix, Hoelen, Lycii Fructus, Paeoniae Radix, Rehmanniae Radix Preparata and Atractylodis Rhizoma, Zingiberis Rhizoma, Zizyphi Semen, Acori Graminei Rhizoma) that have been widely used for the treatment of inflammatory diseases in Asia. Since our previous study has been shown to have the anti-inflammatory activity of CML-1 in vivo and the upregulation of adhesion molecules in response to numerous inducing factors is associated with inflammation, this study examined the effect of CML-1 on the expression of adhesion molecules induced by TNF-alpha in cultured human umbilical vein endothelial cells (HUVECs). Preincubation of HUVECs for 20h with CML-1 (1-100mug/ml) dose-dependently inhibited TNF-alpha (10ng/ml)-induced adhesion of THP-1 monocytic cells, as well as mRNA and protein expression of E-selectin, vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1). CML-1 was also shown to inhibit NK-kB activation induced by TNF-alpha. Furthermore, CML-1 inhibited TNF-alpha-induced IkB kinase activation, subsequent degradation of IkBalpha, and nuclear translocation of NK-kB. Evidence presented in this report demonstrated that CML-1 inhibited the adhesive capacity of HUVEC and the TNF-alpha-mediated induction of E-selectin, ICAM-1 and VCAM-1 in HUVEC by inhibiting the IkB/NF-kB signaling pathway at the level of IkB kinase, which may explain the ability of CML-1 to suppress inflammation and modulate the immune response.     

Gastric antisecretory and antiulcer activities of Cedrus deodara (Roxb.) Loud. in Wistar rats

Ethnopharmacological relevance: Cedrus deodara (Roxb.) Loud. is used in Ayurvedic medicine to treat peptic ulcer.

Aim of the study

To evaluate the gastric antisecretory and antiulcer activity of Cedrus deodara.

Materials and methods

The volatile oil extracted by steam distillation of Cedrus deodara wood was examined for its gastric antisecretory and antiulcer effect in the pylorus-ligated rat model and ethanol induced gastric lesions in rats.

Results

The volatile oil showed significant antisecretory activity as evidenced by decreased gastric fluid volume, total acidity, free acidity and increase in the pH of the gastric fluid in pylorus-ligated rats. Our studies also revealed that pretreatment with Cedrus deodara significantly reduced the number of ulcer, ulcer score and ulcer index in pylorus-ligated and ethanol treated rats. The antiulcer activity of Cedrus deodara is further supported by histopathological study which showed protection of mucosal layer from ulceration and inflammation.

Conclusion

The present findings conclude that volatile oil of Cedrus deodara wood has potent antisecretory and antiulcer effects and justify the traditional usage of this herb to treat peptic ulcers.     

Inhibitory effects of several flavonoids on E-selectin expression on human umbilical vein endothelial cells stimulated by tumor necrosis factor-alpha

The present study investigates the suppressive effect of flavonoids on TNF-alpha-stimulated E-selectin expression on HUVECs by carrying out a comparative examination of the 37 flavonoids. Several flavonoids: fisetin, luteolin and apigenin (subclass of flavone), kaempferol and quercetin (flavonols), eriodictyol (flavanones), genistein (isoflavones) and butein (chalcone) exhibit the inhibitory effects. Considerations to the structure of flavonoids, the C2-C3 double bond of C-ring and 4-oxo functional group are essential for their inhibition activities. These results help to explain the pharmacological efficacy of flavonoids as anti-inflammatory compounds.     

Chrysanthemum morifolium Ramat. reduces the oxidized LDL-induced expression of intercellular adhesion molecule-1 and E-selectin in human umbilical vein endothelial cells

Ethnopharmacological relevance

The flower of Chrysanthemum morifolium Ramat. (CM) with antioxidant, cardiovascular protective and anti-inflammatory functions, has been widely used in China for hundreds of years as a healthy beverage and medicine.

Aims of the study

The purpose of the present study is to investigate the effects of HCM (a hot water extract of the flower of Chrysanthemum morifolium Ramat. [CM]), ECM (an ethanol extract of CM), and the abundant flavonoids apigenin and luteolin in CM on the oxidized LDL (oxLDL)-induced expression of ICAM-1 and E-selectin in human umbilical vein endothelial cells (HUVECs). The possible mechanism of these effects was also determined.

Materials and methods

MTT assay was for cell viability. Western blot was used for ICAM-1 and E-selection protein expression, and for activation of protein kinase B (PKB) and cAMP responsive element binding protein (CREB) proteins. Fluorescence flow cytometry was for ICAM-1 and E-selectin expression on cell surface. DCF-DA flow cytometric assay was used for reactive oxygen species (ROS) production.

Results

HCM, ECM, apigenin, and luteolin dose-dependently inhibited ICAM-1 and E-selectin expression and adhesion of HL-60 by oxLDL. HCM, ECM, apigenin, and luteolin reversed the inhibition of phosphorylation of Akt and CREB by oxLDL; however, this reversion was abolished by wortmannin. In addition, wortmannin abrogated the inhibitory effects of CM extracts, apigenin and luteolin on adhesion molecule expression. The ROS scavenging capability of HCM, ECM, apigenin, and luteolin proceeded dose-dependently in the presence of oxLDL.

Conclusion

CM is a plant with cardiovascular-protective potential and the inhibitory effects of CM on ICAM-1 and E-selectin expression are, at least partially, attributed to its antioxidant activity and modulation of the PI3K/Akt signaling pathway.     

Inhibitory effects of the Gentiana macrophylla (Gentianaceae) extract on rheumatoid arthritis of rats

Acute treatment of rheumatoid rats with an extract from the roots of Gentiana macrophylla (Gentianaceae) produced a significant inhibitory effect on rheumatoid arthritis (RA). When rats were administered the Gentiana macrophylla extract orally at a daily dose of 100 mg/kg body weight, the prostaglandin E(2) (PGE(2)) levels in the inflammatory tissues, sole thickness, and ankle circumferences of feet were significantly decreased. The anti-inflammatory activity observed in Gentiana macrophylla is comparable to that observed in prednisone. These observations suggest that Gentiana macrophylla displays considerable potency in anti-inflammatory action and could be used as an anti-inflammatory agent in the control of inflammation of rheumatoid arthritis.     

生肌象皮膏对糖尿病大鼠溃疡愈合中VCAM-1与ICAM-1的影响

[目的] 探讨生肌象皮膏促进糖尿病大鼠溃疡创面愈合的免疫-炎症反应机制。[方法] 将Wistar大鼠随机分为4组,1组为正常对照组,其余3组采用STZ尾静脉注射制备糖尿病模型,4组动物均于背部打孔,造成溃疡模型。3组糖尿病溃疡大鼠按照血糖和体质量分层随机分为糖尿病对照组、凡士林组、生肌象皮膏组。生肌象皮膏组外用生肌象皮膏纱条外敷,凡士林组用凡士林纱条外敷,正常对照组、糖尿病对照组均以生理盐水纱条外敷,观察创面组织血管细胞黏附分子（VCAM-1）与细胞间黏附分子-1（ICAM-1）表达水平的变化。[结果] 生肌象皮膏组大鼠糖尿病溃疡创面的愈合速度加快,与糖尿病对照组比较于第7、14、21、30天有统计学差异;生肌象皮膏组大鼠溃疡创面中VCAM-1表达水平与糖尿病对照组比较于第14、21、30天降低,有统计学差异（P<0.05）,与凡士林组比较第14、21、30天降低,有统计学差异（P<0.05）;糖尿病对照组大鼠溃疡创面中VCAM-1表达水平与正常对照组比较于第21、30天升高,有统计学差异（P<0.05）;生肌象皮膏组大鼠创面组织ICAM-1表达水平与糖尿病对照组比较于第14、21、30天降低,有统计学差异（P<0.01）,与凡士林组比较第21、30天降低,有统计学差异（P<0.05）;与正常对照组比较,糖尿病对照组大鼠创面组织ICAM-1表达水平于第14、21、30天升高,有统计学差异（P<0.05）。[结论] 生肌象皮膏促进糖尿病大鼠溃疡创面的愈合与其调节免疫-炎症反应有密切联系。     

Andrographolide inhibits the tumour necrosis factor-α-induced upregulation of ICAM-1 expression and endothelial-monocyte adhesion

Andrographolide, a diterpene isolated from Andrographis paniculata, has been shown to have several biological activities including analgesic, antipyretic and antiinflammatory effects. Since the upregulation of adhesion molecules expression and endothelial-leucocytes adhesion are key steps in the development of inflammation, the present study examines whether andrographolide modulates these biological processes in vitro. Incubation of endothelial cells with non-toxic concentrations (0.16–16.7 υg/mL) of andrographolide attenuated the tumour necrosis factor-α (TNF)-induced intercellular adhesion molecule-1 (ICAM-1) expression. Similar concentration ranges of andrographolide also inhibited the TNF-induced endothelial-monocyte adhesion in a concentration-dependent manner. These effects of andrographolide may account for its reported in vivoantiinflammatory activity. © 1998 John Wiley & Sons, Ltd.     

黄芪卫矛合剂对糖尿病肾病大鼠肾小球内皮细胞VACM-1表达的影响

目的:从影响糖尿病肾病内皮细胞血管细胞粘附分子-1(VACM-1)的角度,探讨黄芪卫矛合剂对糖尿病肾病的治疗作用和机制。方法:采用单侧肾切除并链脲佐菌素尾静脉注射建立大鼠糖尿病肾病模型,随机分为假手术组、糖尿病肾病组、糖尿病肾病+黄芪卫矛合剂组和糖尿病肾病+氯沙坦钾组。观察12周末各组大鼠体重、尿量、饮水量、尿蛋白排泄率、血糖、糖化血蛋白、血肌酐、尿素氮、Ⅳ型胶原蛋白、层粘连蛋白的变化。用免疫组织化学染色法检测肾小球VACM-1和α-SMA的表达。结果:黄芪卫矛合剂按40g(生药)/kg给药和氯沙坦钾按16mg/kg给药可减少模型组大鼠饮水量、尿蛋白排泄率和层粘连蛋白,降低VACM-1和α-SMA在肾小球内表达。黄芪卫矛合剂还可减少模型组大鼠尿量,降低血糖、糖化血红蛋白、血脂,在减少尿量和降低糖化血红蛋白方面优于氯沙坦钾组。结论:黄芪卫矛合剂可减少糖尿病肾病大鼠肾组织中VACM-1表达,抑制内皮细胞的表型转化,保护肾脏内皮细胞,从而对糖尿病肾病大鼠肾组织有保护作用。     

匙羹藤降血糖成分研究进展

匙羹藤来源于萝(艹摩)科植物匙羹藤Gymnema syl-vestre(Retz.)Schult(简称GS)的干燥茎及叶。又称武靴藤、羊角藤、金刚藤。广泛分布印度、越南及我国的广西、广东、福建、云南和台湾等省区。目前,我国已确定的有9种匙羹藤的同属近缘植物,包括宽叶匙羹藤G.latifolium Wall.ex Wight、云南匙羹藤C yunnanenes Tsiang、广东匙羹藤G.tingens Spreng、海南匙羹藤C.hainanense Tsians、会东藤G.longiretinaculatum Tsiang等。匙羹藤在民间应用已久,传统认为其性平,味苦,具有止痛、生肌、消肿之功效,主治多发性脓肿。印度民间用于抗肿毒、蛇伤、解疟、利尿及降血糖等。近年来国外学者发现匙羹藤有降血糖、抗龋及抑制甜味反应等作用,在日本、美国、欧洲申请有20多项专利。近几年来,国内学者对匙羹藤降血糖活性成分。精制工艺及药理作用也进行一些研究。     

泄浊化瘀降脂灵对高血脂症大鼠肝脏ICAM-1、MCP-1表达的影响

目的通过观察泄浊化瘀降脂灵对高血脂症大鼠的血脂以及肝脏黏附分子-1(ICAM-1)、单核细胞趋化因子-1(MCP-1)表达的影响,探讨泄浊化瘀降脂灵对高脂血症大鼠肝脏的保护机制。方法将大鼠随机分成空白组、模型组、辛伐他汀组以及浊化瘀降脂灵高、低剂量组,采用高脂饲料复制高血脂症大鼠模型。应用酶法检测血中TC、TG、HDL-C、LDL-C的含量,光镜观察肝脏形态学的改变,免疫组织化学法观察肝组织细胞间ICAM-1、MCP-1的蛋白表达。结果①与空白组比较,模型组造模后TC、TG、LDL-C水平显著升高(P<0.05或0.01),HDL-C水平显著降低(P<0.01),提示各组大鼠造模成功。②与〗模型组比较,泄浊化瘀降脂灵高剂量组、辛伐他汀组TC、TG及LDL-C水平显著降低(P均<0.01),HDL-C水平显著升高(P<0.01)。泄浊化瘀降脂灵高、低剂量组比较亦有显著性差异(P均<0.05)。③与模型组比较泄浊化瘀降脂灵高剂量组及辛伐他汀组肝脏ICAM-1、MCP-1的OD值均显著降低(P均<0.01)。泄浊化瘀降脂灵高剂量组与辛伐他汀组比较无显著性差异(P均>0.05)。结论泄浊化瘀降脂灵高剂量可下调MCP-1及ICAM-1高表达,减少、减轻肝细胞脂肪变性和炎症反应,保护高血脂症大鼠的肝脏。     

肾疏宁对肾小球系膜细胞ICAM-1及其mRNA表达的影响

目的 :探讨中药肾疏宁对系膜细胞分泌细胞间粘附分子的作用。方法 :应用细胞培养技术和血清药理学方法 ,采用血管紧张素Ⅱ作为一种刺激因子 ,观察肾疏宁血清对血管紧张素Ⅱ条件系膜细胞分泌ICAM - 1的影响。结果 :系膜细胞受到血管紧张素Ⅱ刺激后 ,ICAM - 1的分泌显著增加 ,而肾疏宁血清能明显抑制ICAM- 1的分泌和表达。结论 :肾疏宁对粘附分子介导的病理损伤过程有明显的抑制作用。