RP-HPLC法测定盐酸利多卡因注射液含量-中国药典(2000版)和美国药典(27版)方法的比较

目的用反相高效液相色谱法(RP-HPLC)测定盐酸利多卡因注射液含量,并将中国药典(2000版)与美国药典(27版)两种方法进行比较. 方法用两国药典方法(反相高效液相色谱法)测定盐酸利多卡因注射液含量.中国药典(2000版)色谱条件:C18柱,以醋酸钠溶液-甲醇(40:60)为流动相,检测波长254 nm.美国药典(27版)色谱条件:C18柱,以醋酸钠溶液-乙腈(4:1)为流动相,检测波长254nm.结果中国药典(2000版)方法盐酸利多卡因在0.1～3.0 g·L-1浓度范围内,浓度与峰面积线性关系良好,相关系数r=0.999 9.美国药典(27版)方法盐酸利多卡因在0.1～3.0 g·L-1浓度范围内,浓度与峰面积线性关系良好,相关系数r=0.999 8.结论二者盐酸利多卡因浓度与峰面积均具有良好的线性关系,峰面积相差不大,无明显差别.     

RP-HPLC法测定盐酸利多卡因注射液含量—中国药典(2005版)和美国药典(30版)的方法比较

目的用反相高效液相色谱法（RP-HPLC）测定盐酸利多卡因注射液含量,并将中国药典（2005版）与美国药典（30版）两种方法进行比较。方法用两国药典方法（反相高效液相色谱法）测定盐酸利多卡因注射液含量。中国药典（2005版）色谱条件：C18柱,以磷酸盐缓冲液-乙腈（50∶50）,用磷酸调节PH至8.0为流动相,检测波长254 nm。美国药典（30版）色谱条件：C18柱,以醋酸钠溶液-乙腈（4∶1）为流动相,检测波长254 nm。结果中国药典（2005版）方法盐酸利多卡因在0.1-2.4 g·L^-1浓度范围内,浓度与峰面积线性关系良好,相关系数r=0.9996。美国药典（27版）方法盐酸利多卡因在0.1-2.4g·L^-1浓度范围内,浓度与峰面积线性关系良好,相关r=0.9998。回收率、精密度两者无显著差异。结论两者盐酸利多卡因浓度与峰面积均具有良好的线性关系。     

法莫替丁氯化钠注射液中法莫替丁含量测定方法的改进

用高效液相色谱法,测定法莫替丁氯化钠注射液中法莫替丁的含量。十八烷基硅烷键合硅胶(5μm)为分析柱。流动相:庚烷磺酸钠溶液(取庚烷磺酸钠2.0g,加水900mL溶解后,用冰醋酸调节pH值为3.9,加水至1000mL)-乙腈-甲醇(25∶6∶1);流速:1.0mL.min-1;检测波长254nm。在进样量为20μL时,法莫替丁在进样浓度50~200μg·mL-1的范围内,峰面积与进样浓度之间呈良好的线性关系。(r=0.9998),重复进样RSD=0.68%。     

HPLC法测定盐酸林可霉素含量——中国药典(2005版)和美国药典(27版)方法的比较

目的用高效液相色谱法(HPLC)测定盐酸林可霉素含量,并将中国药典(2005版)与美国药典(27版)两种方法进行比较。方法用两国药典方法测定盐酸林可霉素含量。中国药典(2005版)色谱条件:C1 8柱,以0.05 mol.L-1硼砂溶液(85%磷酸溶液调节pH值至6.0)-甲醇(4∶6)为流动相,检测波长214 nm,待测溶液浓度2.0 g.L-1;美国药典(27版)色谱条件:C8柱,以磷酸溶液-甲醇-乙腈(780∶150∶150)为流动相,检测波长210 nm,待测溶液浓度1.2 g.L-1。结果中国药典(2005版)方法盐酸林可霉素在浓度范围内,浓度与峰面积线性关系良好,相关系数r=0.9999;美国药典(27版)方法盐酸林可霉素在浓度范围内,浓度与峰面积线性关系良好,相关系数r=0.9997。平均回收率在99.0%~101.0%,RSD均小于0.5%结论二者盐酸林可霉素浓度与峰面积均具有良好的线性关系,含量相差不大,无明显差别。     

法莫替丁制剂有关物质检查

目的探讨用高效液相色谱（HPLC）法测定法莫替丁制剂的有关物质的量。方法采用Waters C18色谱柱．流动相为0．2％庚烷磺酸钠（用冰醋酸调pH值至3．9）-乙腈-甲醇（26：6：1），检测波长为254am。结果法莫替丁质量浓度为0．240-1．683μg／mL时与峰面积线性关系良好（r=0．9997）。结论HPLC法可用于法莫替丁制剂的有关物质测定，方法简便、准确、稳定。     

法莫替丁氯化钠注射液质量标准的相关研究

目的建立法莫替丁氯化钠注射液中法莫替丁含量及有关物质检测的高效液相色谱方法.方法采用Agilent ZORBAX SB-C18(4.6×250mm,5μm)色谱柱,以0.2%(g·L-1)庚烷磺酸钠溶液(用冰醋酸调pH值为3.7)-乙腈-甲醇(33∶7∶3)为流动相,流速1.2mL·min-1,柱温为35℃,检测波长为254nm.结果法莫替丁的浓度在0.0794～0.238mg·L-1范围内有良好的线性关系,r=0.9999,样品的平均回收率为99.58%,RSD为0.91%.结论高效液相色谱法测定法莫替丁氯化钠注射液中的法莫替丁含量和有关物质,方法准确度、精密度高,适合应用于该制剂的质量控制.     

HPLC法测定枸橼酸莫沙比利片的含量

目的 建立测定枸橼酸莫沙比利片含量的HPLC方法。方法采用ODS色谱柱,磷酸二氢钾、庚烷磺酸钠溶液(取 磷酸二氢钾2．72g与庚烷磺酸钠1．0g加水溶解并稀释成1000ml)-乙腈(50:50)为流动相,检测波长为274nm。结果枸橼 酸莫沙比利在5μg·ml-1～50μg·ml-1范围内峰面积与浓度呈良好线性关系(r=0．9998),平均回收率为98．8％(RSD= 1．3％)n=6。结论 HPLC法测定枸橼酸莫沙比利含量,方法准确,重复性好,回收率高。     

高效液相色谱法测定法莫替丁氯化钠注射液的含量

目的:建立一种法莫替丁氯化钠注射液高效液相色谱测定方法,用于生产及市场监控。方法:选用C_(18)(150×4.6mm)5μm色谱柱,庚烷磺酸钠-乙腈-甲醇(25:6:1)为流动相,以外标法按峰面积计算。结果:该法回收率为99.84%,表明用该法测定法莫替丁氯化钠注射液的含量是可行的。结论:该法简便、快捷,结果准确,可用于法莫替丁氯化钠注射液的含量测定和质量控制。     

法莫替丁注射液有关物质的测定

目的：建立高效液相色谱法,测定法莫替丁注射液样品的有关物质。方法：色谱柱为Hypersil ODS2（250mm×4.6mm,5μm）;流动相为庚烷磺酸钠溶液（取庚烷磺酸钠2.0g,加水900ml溶解后,用冰醋酸调节pH至3.9,加水至1000ml,摇匀）-乙腈-甲醇（26∶7∶1）;流速为1.0ml/min;检测波长为254nm;进样量为20μl。结果：有关物质测定法专属性强;10批样品平均有关物质为1.15%,RSD=3.5%（n=10）。结论：方法简便、快速、准确且专属性强,可作为法莫替丁注射液有关物质的质量控制方法。     

HPLC法测定法莫替丁葡萄糖注射液中5-羟甲基糠醛的含量

目的建立一种快速、灵敏、准确的高效液相色谱法测定法莫替丁葡萄糖注射液中5-羟甲基糠醛的含量。方法HPLC法,以HypersilODS2 C18(4.6mm×250mm,5μm)为色谱柱,0.01 m o l.L-1庚烷磺酸钠溶液为流动相,流速1.0m l.m in-1,检测波长为284nm。结果5-羟甲基糠醛在0.0456μg.mL-1~0.456μg.mL-1浓度范围与峰面积有良好线性关系,r=0.9994。平均回收率为99.4%。RSD=0.91%(n=9)。结论本方法准确、简便、快捷,排除了法莫替丁的干扰,可用于测定法莫替丁葡萄糖注射液中5-羟甲基糠醛含量。     

Validity of the substance abuse subtle screening inventory-adolescent version (SASSI-A)

The construct and convergent validity of the Substance Abuse Subtle Screening Instrument-Adolescent (SASSI-A) were examined in a population of suburban youth offenders. The analysis was conducted on archived data of 490 adolescent offenders from a suburban Circuit Court-Juvenile Division. Exploratory factor analysis results best supported a 5-factor solution that accounted for a modest 32.5% of the total variance. This 5-factor solution had a poor goodness of fit with the purported factor structure upon which the formal SASSI-A scoring is based. The relationship between the SASSI-A and variables with known relationships to adolescent substance abuse (i.e., lack of religious involvement, single parent household, learning disability, early onset of use, conduct problems) was examined. Results revealed moderately strong relationships between the SASSI-A and these variables, indicating reasonable convergent validity. It is concluded that the SASSI-A face valid scales have moderate utility for identifying substance dependence within this sample, while the subtle scales do not.     

Psychometric properties of the Turkish version of the Internet Addiction Test (IAT)

ObjectiveOf many instruments developed to assess Internet addiction, the Internet Addiction Test (IAT), an expanded version of the Internet Addiction Diagnostic Questionnaire (IADQ), has been the most widely used scale in English and non-English speaking populations. In this study, our aim was to investigate the psychometric properties of short and expanded versions of the IAT in a Turkish undergraduate sample.MethodOverall, 455 undergraduate students from Turkey aged between 18 and 30 participated in the study (63.53% were females). Explanatory and confirmatory factor analytic procedures investigated factor structures of the IADQ and IAT. The Internet Addiction Scale (IAS), Coping Inventory for Stressful Situations (CISS), Obsessive Compulsive Inventory—Revised (OCI-R) and Dissociative Experiences Scale (DES) were administered to assess convergent and divergent validities of the IADQ and IAT. Internal consistency and 15-day test–retest reliability were computed.ResultsIn the factorial analytic investigation, we found a unidimensional factor structure for each measure fit the current data best. Significant but weak to moderate correlations of the IADQ and the IAT with the CISS, OCI-R and DES provided empirical evidence for divergent validity, whereas strong associations with the subscales of the IAS pointed to the convergent validity of Young's Internet addiction construct. Internal consistency of the IADQ was weak (α = 0.67) and of the IAT was high (α = 0.93). Temporal reliability of both instruments was very high (α = 0.81 and α = 0.87; respectively).ConclusionThe IAT revealed promising and sound psychometric properties in a Turkish sample.     

Confirmatory factor analysis of the Brazilian version of the Drinking Motives Questionnaire-Revised (DMQ-R)

The main objective of this study was to evaluate the factor structure of the Brazilian version of the Drinking Motives Questionnaire-Revised (DMQ-R; Cooper, 1994) in a sample of 584 university students. A secondary goal was to investigate the relationships between motives and measures of alcohol use and drinking problems. The DMQ-R assesses four motive dimensions: social, enhancement, coping, and conformity. Confirmatory factor analysis supported a revised four-factor model identical to the original, with the exception of one item that did not load on its intended factor. Relative to coping and conformity motives, enhancement and social motives were strongly related to both alcohol use and drinking problems. Overall results indicate that the factor structure of the Brazilian DMQ-R parallels that observed in North America and Europe using the original English language DMQ-R, despite a distinct pattern of relationships with alcohol use and drinking problems.     

Validation of the Dutch version of the brief young adult alcohol consequences questionnaire (B-YAACQ)

Purpose

The purpose of this study was to validate the Dutch version of the Brief Young Adult Alcohol Consequences Questionnaire (B-YAACQ).

Method

The B-YAACQ was translated into the Dutch language and 800 students were invited to complete the questionnaire. A number of drinking behaviors were also assessed; including weekly drinking, age of drinking onset, and blood alcohol concentration on a typical night out. Students also completed a short version of the Alcohol Use Disorders Identification Test (AUDIT-PC).

Results

Data from 667 undergraduate and graduate students (184 men and 483 women) who reported alcohol use during the past year was used in the analysis. On average, students in this study report 4.7 alcohol-related consequences. The Dutch B-YAACQ was shown to have a high reliability and validity: Cronbach's Alpha was 0.816, and B-YAACQ scores correlated significantly with AUDIT-PC scores (r = 0.747). B-YAACQ scores correlated significantly (p < 0.01) with various drinking variables, including drinking onset age, drinking days per week, alcoholic drinks per week, number of drinks on a night out and blood alcohol concentration when returning home.

Conclusion

The Dutch B-YAACQ is a useful new tool for screening of alcohol-related consequences.     

Reliability and validity of the German version of the European Addiction Severity Index (EuropASI)

OBJECTIVE: This study provides data on the psychometric characteristics of the German version of the European Addiction Severity Index (EuropASI). The ASI is a frequently used clinical and research instrument that measures problem severity among people with substance dependence. METHOD: The German ASI was used in a sample of 112 consecutively admitted male psychiatric inpatients seeking treatment for severe alcohol problems. The conceptual structure of the German ASI subscales was investigated by analyzing the intercorrelations of the severity ratings and composite scores. Internal consistency, interrater reliability and concurrent validity in terms of correlations with other assessment instruments were evaluated. RESULTS: The German ASI subscales proved to be independent or moderately correlated (-0.17 < r < 0.34). Each correlation coefficient between corresponding severity ratings and composite scores was significant (p < .0005), ranging from r = 0.47 to r = 0.93. Reliability measures indicated moderate to good internal consistency (Cronbach's alpha: 0.69-0.92) and moderate to excellent interrater reliability (intraclass correlation coefficient: 0.62-0.99). Validity was supported by significantly higher ratings in the alcohol section in alcohol dependent patients compared to patients without dependence (t = 2.99, 108 df, p = .004). Significant correlations (p < .001) were found between the alcohol use section and the Michigan Alcoholism Screening Test (r = 0.34 composite score and r = 0.44 severity rating) and between psychiatric status and the Symptom Checklist-90-revised (r = 0.55/0.51), supporting concurrent validity. CONCLUSIONS: The German version presented evidence of acceptable psychometric properties and its applicability in German-speaking countries could be confirmed.