脑胶质细胞瘤患者手术前,后自然杀细胞（NK）活性测定及其…

报告１４例脑胶质细胞瘤患者术前、术后外周血淋巴细胞中自然杀伤细胞（ＮＫ）活性检测结果，并与７例颅骨成形术患者对照，结果显示：（１）脑胶质瘤患得的ＮＫ活性较颅骨成形术患者降低（Ｐ＜０．０５）。（２）脑胶质瘤患乾术后３－６个月，ＮＫ细胞活性明显回升（Ｐ＜０．０１），甚至有的病例恢复到正常水平。（３）激素对ＮＫ活性有明显抑制作用（Ｐ＜０．０１）。（４）脑胶质瘤复发１例ＮＫ活性回升不明显。     

AFP在肝细胞性肝癌手术前后对NK细胞活性的影响及意义

对３７例肝细胞性肝癌患者手术前、后ＮＫ细胞活性进行动态观察。结果表明，肝细胞性肝癌患者的ＮＫ细胞活性低于正常对照组（Ｐ＜０．０１），ＡＦＰ阳性者低于ＡＦＰ阴性者（Ｐ＜０．０１）。术后他们的ＮＫ细胞活性进一步降低，且ＡＦＰ阳性者恢复较慢。提示ＡＦＰ对ＮＫ细胞活性有抑制作用，对肝细胞性肝癌患者（特别是ＡＦＰ阳性者），迅速提高术后机体ＮＫ细胞活性将有助于提高疗效。     

口腔颌面部肿瘤患者血清sIL－2R_α水平及NK活性的测定

测定了２９例口腔颌面部肿瘤患者的自然杀伤细胞（ＮＫ）活性及血清可溶性白细胞介素Ⅱ受体（ｓＩＬ－２Ｒ_α）的水平，结果表明：口腔颌面部恶性肿瘤患者的ＮＫ活性明显低于正常人（Ｐ＜０．０１）和口腔颌面部良性肿瘤患者（Ｐ＜０．０１），恶性肿瘤组血清ｓＩＬ－２Ｒ_α水平又明显高于良性肿瘤组（Ｐ＜０．０１）和正常对照组（Ｐ＜０．０１），且恶性肿瘤组ＮＫ活性与血清ｓＩＬ－２Ｒ_α水平呈负相关（ｒ＝－０．９３５，Ｐ＜０．０１），提示：ｓＩＬ－２Ｒ_α、ＮＫ活性可作为监测口腔颌面部恶性肿瘤病人免疫功能的指标。     

肝癌患者外周血NK和LAK活性及T细胞亚群的变化

采用３ＨＴｄＲ释放法测定了２０例肝癌患者的外周血自然杀伤细胞（ＮＫ）活性及淋巴因子激活的杀伤细胞（ＬＡＫ）活性，同时用ＡＢＣ法检测其外周血Ｔ细胞亚群变化。结果显示，肝癌患者的外周血ＮＫ和ＬＡＫ活性显著低于正常人（Ｐ＜０．０１），Ｔ细胞亚群ＣＤ３及ＣＤ４细胞减少（Ｐ＜０．０１），ＣＤ８细胞增多（Ｐ＜０．０１），ＣＤ４／ＣＤ８比值显著降低（Ｐ＜０．０１）且出现倒置。上述结果提示肝癌患者细胞免疫功能出现紊乱及免疫活性细胞功能有缺陷。     

膀胱癌患者外周血NK细胞活性的研究

本研究测定了３２例膀胱癌和２６例前列腺增生症患者外周血ＮＫ细胞活性，并将膀胱癌组ＮＫ细胞活性分别与正常对照组及前列腺增生症组进行比较，其结果表明膀胱癌患者的ＮＫ细胞活性（３８．９１±２．２３％）比对照组（５０．９１±１．６６％）明显低，（Ｐ＜０．０１）与前列腺增生症组（４９．２７±２．３０％）相比亦有显著差异（Ｐ＜０．０１）。     

锌对老年人自然杀伤细胞活性的影响

应用原于吸收光谱法和１２５ＩＵＲ释放法测定外周血血浆锌值和自然杀伤（ＮＫ）细胞活性，结果表明，老年组显著低于青年组（Ｐ＜０．０１和Ｐ＜０．０５）；老年锌补充组两项均明显增高（Ｐ＜０．０１和Ｐ＜０．０５），且此两组血浆锌值和ＮＫ细胞活性均呈正相关关系（ｒ＝０．５４１，Ｐ＜０．０５和ｒ＝０．７８１，Ｐ＜０．０５）．提示，锌可能对ＮＫ细胞活性的恢复有着调节作用。     

鼻咽癌外周血NK细胞活性的分析研究

作者测定了１１７例鼻咽癌患者外周血ＮＫ细胞活性水平，并与４０例健康者进行比较，结果发现鼻咽癌患者外周血ＮＫ细胞活性低于对照组（Ｐ＜０．０１），低分化鳞状细胞癌、未分化细胞癌患者ＮＫ细胞活性显著低于中分化鳞状细胞癌和对照组（Ｐ＜０．０５和Ｐ＜０．０１）；ＵＩＣＣ－ＴＮＭ分期Ⅱ、Ⅲ、Ⅳ期和复发病例的ＮＫ细胞活性均数各为２５．７５±１４．６３、２４．７０±１２．４２、１９．１５±８．２９、１５．６５±７．２５，Ⅳ期和复发者显著低于Ⅱ、Ⅲ期者（Ｐ＜０．０５）。血ＮＫ细胞活性与鼻咽癌患者血清ＥＢ病毒ＶＣＡ－ＩｇＡ抗体水平呈负相关（γｓ＝－０．８５，Ｐ＜０．０５）。结果表明鼻咽癌患者外周血ＮＫ细胞活性受抑制，并随病情进展而下降。作者认为检测外周血ＮＫ细胞活性可作为评估鼻咽癌预后的一项辅助指标。     

心肌梗塞患者自然杀伤细胞活性检测

用１２５ＩＵｄＲ释放法测定１７例急性心肌梗塞（ＡＭＩ）后一周内的外周血自然杀伤（ＮＫ）细胞活性，并与１３例陈旧性心肌梗塞（ＯＭＩ）及５８例正常人对照。结果表明：ＡＭＩ组ＮＫ细胞活性明显低于正常组及ＯＭＩ组（Ｐ＜０．０１）。ＡＭＩ组中，梗塞面积广泛者ＮＫ细胞活性明显低于梗塞面积局限者（Ｐ＜０．０１）。提示ＮＫ细胞活性可作为ＡＭＩ诊断和预后判断的一个辅助参考指标。     

自体LAK细胞回输疗法对慢性乙型肝炎患者NK及LAK细胞活性的影响

目的探讨自体ＬＡＫ细胞回输疗法的作用机制。方法用ＭＴＴ比色法检测２９例慢性活动型乙型肝炎（ＣＡＨ）患者及１４例正常人外周血ＮＫ及ＬＡＫ细胞活性；检测２１例自体ＬＡＫ细胞回输治疗前、后ＣＡＨ患者外周血ＮＫ及ＬＡＫ细胞活性。结果ＣＡＨ患者ＮＫ及ＬＡＫ细胞活性较正常人低，差异有显著性（Ｐ＜０．０１）；治疗后较治疗前ＮＫ及ＬＡＫ细胞活性增高，差异有显著性（Ｐ＜０．０１）。结论自体ＬＡＫ细胞回输疗法治疗ＣＡＨ有效，其作用机制之一是提高了ＣＡＨ患者的ＮＫ及ＬＡＫ细胞活性。     

老年慢性肺心病患者自然杀伤细胞活性检测及分析

对３２例老年慢性肺心病及３０例正常人外周血自然杀伤细胞（ＮＫ细胞）活性进行检测，结果表明，老年慢性肺心病患者ＮＫ细胞活性在急性加重期或缓解期均明显低于正常人（Ｐ〈０．０１）；且与患者病程长短无关（Ｐ〈０．０５），但急性加重期明显低于缓解期（Ｐ〈０．０１），伴有呼吸衰竭的肺心病患者ＮＫ细胞活性下降更为显著。提示慢性肺心病患者存在着细胞免疫功能障碍，ＮＫ细胞活性检测可以作为判断肺心病病情及预后的一个…     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.