Relationship between the Expression of MTA-1 Gene and the Metastasis and Invasion in Human Osteosarcoma

Summary：To compare the expression level of metastasis associated-1 （MTA 1 ） gene in high and low metastatic： human osteosarcoma cell lines and examine the relationship of MTA 1 expression and the metastasis potentiality of osteosarcoma cells, the expression of MTA 1 in MC-63 osteosarcoma cell lines with high and low metastasis potential was detected by semiquantitative TR-PCR. Boyden chamber invasion assay was used to evaluate the invasive capacity in vitro in two osteosarcoma cell lines, The low metastasis MG-63 cells were transfected with MTA 1 full-length cDNA expression plasmid by lipofectamine and the changes of MTA 1 expression and in vitro invasion potential were examined after the transfection. Our results showed that MG63 cell line with high metastasis potential expressed significantly higher MTA 1 than that of MG63 cells with low metastasis as reavealed by RT-PCR The invasion potential of low metastasis MG63 cell line was increased after MTA 1 gene transfection. It is concluded that there may be a relationship between MTA 1 and invasive potentiality of human osteosarcoma cells, and the mechanism of MTA 1 in osteosarcoma metastasis and its possible role in associated gene therapy deserve further study.     

AduoLa Fuzhenglin Down-regulates Microwave-induced Expression of β_1-adrenergic Receptor and Muscarinic Type 2 Acetylcholine Receptor in Myocardial Cells of Rats

<正>This paper is aimed to study the effect of ADL on expression ofβ1-AR and M2-AchR in myocardial cells of rats exposed to microwave radiation.Immunohistochemistry,Western blot and image analysis were used to detect the expression ofβ1-AR and M2-AchR in myocardial cells at 7 and 14 d     

Gene Expression of Adiponectin and Adiponectin Receptor 1 in Type 2 Diabetic Rats and the Relationship with the Parameters of Glucose and Lipid Metabolism

Adiponectin is an adipocyte-derived factor ,which plays pivotal roles of the insulin-sensitizing,anti-inflammatory ,and atheroprotective . Two adi-ponectin receptor types were recently identified:AdipoR1 is abundantly expressed in muscle ,whereas AdipoR2 is predominantly expressed inthe liver[1].They are thought totransmit effects ofadiponectin . We examined serumadiponectin, mR-NAlevels of adiponectinin adipose tissue and mR-NAlevels of AdipoR1 inthe muscles of type 2 dia-betic rats ,to …     

Gene transfer of a β2-adrenergic receptor kinase inhibitor up-regulates the level of β2-adrenergic receptor and cAMP in the asthmatic murine lung

Objective: To investigate the effects of gene transfer of a β-adrenergic receptor(β-AR) kinase inhibitor(β ARKct)on pulmonary β2-adrenergic receptor and cAMP following β2-AR agonist treatment in asthmatic mice, and to analyze the relationship between the routes of gene delivery and the changes of β2AR and cAMP. Methods: BALB/c mice were sensitized and challenged by ovalbumin to establish the asthmatic model treated with βAR agonist ( salbutamol injected intramuscularly). The plasmid with the expression of βARKct was constructed and βARKct gene transfer was performed through intravenous injection or intratracheal instillation in asthmatic mice.The gene expression was measured with Western blot analysis, and the changes of pulmonary β-AR and cAMP evaluated by Radioimmunoassay. Results: The expression of tranfered βARKct gene was detectable in lungs and it was expressed more in the lungs of the mice receiving intratracheally plasmid than those receiving intravenously. The levels of βAR and cAMP were upregulated after using plasmid-βARKct to the asthmatic mice treated with β AR agonist. Conclusion: Our results indicated that there were down-regulation of βAR and cAMP in asthmatic mice treated with βAR agonist. Gene transfer of βARKct could inhibit the extent of the down-regulation of βAR and cAMP. The route of gene delivery could also affect the degree of up-regulation of βAR and cAMP. Gene transfer βARKct may provide a novel approach to the therapeutic strategy for asthma.     

Gene transfer of a β2-adrenergic receptor kinase inhibitor up-regulates the level of β2-adrenergic receptor and cAMP in the asthmatic murine lung

Objective： To investigate the effects of gene transfer of a β-adrenergic receptor（β-AR） kinase inhibitor（β ARIct） on pulmonary β2-adrenergic receptor and cAMP following β2-AR agonist treatment in asthmatic mice, and to analyze the relationship between the routes of gene delivery and the changes of β2AR and cAMP. Methods： BALB/c mice were sensitized and challenged by ovalbumin to establish the asthmatic model treated with βAR agonist （salbutamol injected intramuscularly）. The plasmid with the expression of βARKct was constructed and βARKct gene transfer was performed through intravenous injection or intratracheal instillation in asthmatic mice. The gene expression was measured with Western blot analysis, and the changes of pulmonary β-AR and cAMP evaluated by Radioimmunoassay. Results： The expression of tranfered βARKct gene was detectable in lungs and it was expressed more in the lungs of the mice receiving intratracheally plasmid than those receiving intravenously. The levels of βAR and cAMP were upregulated after using plasmid-βARKct to the asthmatic mice treated with βAR agonist. Conclusion： Our results indicated that there were down-regulation of βAR and cAMP in asthmatic mice treated with βAR agonist. Gene transfer of βARKct could inhibit the extent of the down-regulation of βAR and cAMP. The route of gene delivery could also affect the degree of up-regulation of βAR and cAMP. Gene transfer βARKct may provide a novel approach to the therapeutic strategy for asthma.     

Relationship between the Expression of RASSF1A Protein and Promoter Hypermethylation of RASSF1A Gene in Bladder Tumor

To investigate the relationship between the expression of RASSF1A protein and promoter hypermethylation of RASSF1A gene, RASSF1A protein expression was measured by Western blotting in 10 specimens of normal bladder tissues and 23 specimens of bladder transitional cell carcinoma （BTCC）. The promoter methylation in BTCC and normal bladder tissues was detected by methylation-specific PCR （MSP）. The results showed that the expression level of RASSF1A protein was significantly lower in BTCC tissues than that in normal bladder tissues. However, it was not correlated with its clinical stages and pathological grades. The frequency of promoter methylation of RASSF1A gene was higher in BTCC tissues than that in normal bladder tissues. In 14 patients with the aberrant promoter methylation, 13 showed loss or low expression of RASSF 1A protein. It is concluded that RASSF1A gene promoter methylation may contribute to the low level or loss of RASSF1A protein expression, the inactivation of RASSF1A gene and the genesis of BTCC. But, it may bear no correlation with its clinical stages and pathological grades.     

Study on Relationship between Ovulation Inducing Effect of Drug-Acupuncture and Endometrial Contents of Estradiol Receptor and Progesterone Receptor

Study on Relationship between Ovulation Inducing Effect of Drug-Acupuncture and Endometrial Contents of Estradiol Receptor and Progesterone Receptor@田冬珍@谢学鸥@王彬@彭智慧     

Relationship between Trp64Arg mutation in the β3-adrenergic receptor gene and metabolic syndrome: a seven-year follow-up study

Background It has been shown that the β3-adrenergic receptor （β3-AR） gene Trp64Arg mutation was closely related to obesity and insulin resistance, and may be related to the prevalence of metabolic syndrome （MS）. The aim of this study was to investigate the relationship between the 33-AR gene mutation and the prevalence of MS. Methods A seven-year follow-up study was initiated in 2000, with 496 samples of simplex obese subjects （body mass index ≥25 kg/m2） and 248 normal-weight subjects. According to the β3-AR genotypes, the subjects were classified as Trp64 homozygote group and Arg64 carrier group and after 7 years the prevalence of MS was determined. Results According to the baseline profile, there were no significant differences in the adiposity, blood pressure, lipid profile, fasting plasma glucose and fasting insulin between Trp64 homozygote group and Arg64 carrier group either in obesity or normal-weight subjects. The results of follow-up study indicated that in obese men the prevalence rate of MS was much higher in Arg64 carrier group than that in Trp64 homozygote group （54.76% vs. 40.85%, P 〈0.05）, but there was no statistical difference in women of the above groups. The prevalence rate of MS in obese men of both Trp64 homozygote group and Arg64 carrier obese group were obviously higher than that in women of the above groups （40.85% vs. 18.27% and 54.76% vs 21.28%, all P 〈0.005）. Differences were not statistically significant in the prevalence of MS for normal weight Trp64 homozygote group and normal weight Arg64 carrier group, either between men, between women, or between men and women. Comparison of populations indicated that no matter with the β3-AR gene mutation or not, the prevalence of MS in obese subjects was significantly higher than normal weight subjects （X2=28.240 and x2=15.586, all P 〈0.005）. Logistic analysis showed that the mutation of β3-AR gene was associated with the prevalence of MS in men.     

PIN1 Gene Overexpression and β-catenin Gene Mutation/Expression in Hepatocellular Carcinoma and Their Significance

The evolution of hepatocellular carcinoma (HCC) is a compound process which involves many kinds of genes and transductional pathways. The expression of the peptidyl-proplyl- isomerase PIN1 gene, the mutation in exon 3 of β-catenin and its correspondent abnormal expression and their roles in the hepatocellular carcinogeneisis were investigated. Among 29 pair cases of HCC and non-carcinoma tissues, the expression of PIN1 gene was detected by immunochemical staining. Mu-tations in exon 3 of β-catenin gene and differential expression of β-catenin gene were investigated by the methods of PCR-SSCP, direct sequencing and immunohistochemical technique as well. The re-sults indicated: (1) 44.8% (13/29) cases of HCC presented higher level of PIN1 gene expression than non-cancerous tissues (χ2 =32.63, P<0.05), especially in cytoplasm and nucleus, while there was lower level of PIN1 expression in non-cancerous tissues; (2) 58.6% (17/29) HCC tissues showed β-catenin protein accumulation in cytoplasm and nucleus. 46.2% (6/13) HCC tissues indicated β-catenin protein accumulation with higher level of PIN1 expression, while 53.8% (7/13) HCC tis-sues indicated β-catenin protein accumulation with lower level or trace of PIN1 expression (χ2 =0.00, P>0.05); (3) 24.1% (7/29) of primary tumor lesions carried gene mutations in exon 3 of β-catenin, and accompanied by β-catenin protein accumulation. There was no mutation in non-cancerous tissues. All the mutation presented in tissues with low level of PIN1 expression. There was no mutation of β-catenin gene in tissues with high PIN1 expression level (χ2=58.12, P<0.05). So it was postulated that the increase of PIN1 gene expression could promote hepatocellular carcinogenesis via a way dif-ferent from β- catenin gene mutation.     

Exploration of the Relationship between Phlegm-Dampness Constitution and Polymorphism of Low Density Lipoprotein Receptor Genes Pvu Ⅱ and Ava Ⅱ

Objective:To explore the polymorphism of low density lipoprotein receptor (LDL-R) genes PvuⅡand AvaⅡin a population with phlegm-dampness constitution (PDC).Methods: Polymorphism of LDL-R genes at PvuⅡand AvaⅡof 48 persons with gentle constitution (GC) and 61 with PDC were analyzed with PCR-RELP technique,and their serum contents of lipids and glucose were determined and compared as well.Results:The A allelic and P- allelic frequency were higher and the P allelic frequency was lower in subjects with PDC than those in subjects with GC,which were 0.3083 vs 0.1771,0.9098 vs 0.7708 and 0.0902 vs 0.2292,respectively,all showing significant difference between the two groups (P<0.05).Comparison of the two groups in serum levels of triglyceride (TG),fasting blood glucose,2 h postprandial blood glucose,and 2 h postprandial insulin showed that all the parameters were higher in subjects with PDC than in subjects with GC respectively, showing significant difference (P<0.05).Conclusion:PDC is related with the P- and A allelic frequency of higher LDL-R genes at PvuⅡand AvaⅡ,therefore,the polymorphism of LDL-R genes could be taken as one of the genetic markers for PDC,and humans with PDC are more liable to suffer from blood lipids and glucose disorder than those with GC.     

The Relationship between 67KD Laminin Receptor Expression and Metastasis of Hepatocellular Carcinoma

Laminin(LN),themajorglycoproteinofbasememtmembrane,stimulatesattachment,spreading,migrationanddifferentiationoftumorcells.Interactionsbetweentumorcellsandbasementmembranearedeterminantintumorinvasionandmetastasiswhicharemainlymediatedthroughthesurfaceprotein,67KDIamininreceptor(LN--R).Increasedcellexpressionofthisreceptorhasbeencorrelatedwiththeinvasiveandmetastaticcapabilitiesofcancercells[1].Immunohistochemically,ithadbeendemonstratedthatLN--Rwasinvolvedintheinvasiveandmetastaticprocesse…     

Relationship between β3-AR Gene and Obesity, Type 2 Diabetes, Insulin Resistance in Chinese Han Population

Objective: To explore the relationship between the β3-adrenergic receptor(β3-AR)gene and obesity, T2DM. insulin resistance in Chinese Han population. Methods: Fifty-three healthy subjects, 105 subjects with simple obesity, 63 type 2 diabetic patients without obesity, and 114 type 2 diabetic patients with obesity were studied with the technique of PCR-RFLP in codon 64 of the exon region of β3-AR gene representing the variation Trp/Arg. Results:Compared with the subjects of Trp homozygous group, the individuals with Arg allele were more elevated in WHR,MBP,SBP,DBP,FBS,PBS, FINS,PINS, FCP,PCP and lower in ISI. Frequency of Arg allele was higher in HINS sub-group without T2DM. Cnclusion: The results indicate that the Trp/Arg variation might lead to insulin resistance, obesity and T2DM.β3-AR gene is supposed to be the candidate gene of insulin resistance, obesity and T2DM in ChineseHan population.     

The Influence of the Pro12Ala Mutation of PPARγ2 Receptor Gene on β-cells Restoration and Insulin Resistance in Type 2 Diabetes with Hypertension

Type2diabetes(T2DM)isageneraldiseasesduetobothgeneticandenvironmentalfactors[1],andT2DMissusceptibletohypertension(HP).Clinicalandexperimentalstudieshaveindicatedthatinsulinresistanceandhyperinsulinemiaareim portantfactorsresultingindiabetesandhyperten sion.Therefore,geneticfactorscontributealottoT2DMwithhypertension.Uptonow,moststudiesconcerningtheasso ciationbetweenthismutationandsomecomplextraits,suchasobesity,insulinresistanceandT2DM,showedthattheX12Ala(eitherPro12AlaorAla12Ala)gen…     

The Influence of the Prol2Ala Mutation of PPARγ2 Receptor Gene on β-cells Restoration and Insulin Resistance in Type 2 Diabetes with Hypertension

The aim of this investigation was to determine whether a PPAR72 Prol2Ala polymorphism was associated with insulin resistance, β-cellfunction and hypertension in Chinese populations. 289 unrelated Chinese subjects first diagnosed Type 2 diabetes （HbAC1〈6.0） were investigated, including 132 hypertensive diabetic （HTD） subjects, 157 normotensive diabetic （NTD） subjects. Blood pressure and anthropometric measurements were collected from all participants, as well as several venous blood samples during oral glucose tolerance test （OGTT）. Biochemical measurements （high-density lipoprotein （HDL） and low-density lipoprotein-cholesterol （LDL）, triglycerides） and PPARγ2 Pro12Ala genotype were also determined. And insulin resistance and β-cells function was assessed by HOMA-IR and HOMA-β respectively. The frequency of subjects bearing the Pro12Ala was lower in the hypertension group （3. 03 %） than in the non-hypertension group （5.7 %） （P〈0.05） after adjusted for age, BMI and gender. Hypertensive diabetic Pro12Ala subjects had lower fasting plasma glucose level （P=0. 0127）, and better glucose tolerance 60 min after oral glucose （P=0. 0361）. Moreover, plasma insulin concentrations at 60 min was lower than those without A variant （P = 0. 0275）, and both hypertensive Ala/Pro in HOMA-β （P ： 0. 0455） and AUC for insulin （P=0. 0473） were higher, and HOMA-IR was lower （P=0. 0375） as compared with hypertensive Pro/Pro subjects. No association was observed between Prol2Ala genotype and BMI, total cholesterol, HDL- cholesterol or triglycerides in either group. Our findings suggested that the Ala 12 allele of the PPARγ2 gene may improve insulin resistance and ameliorate β-cell function reserves in T2DM with hypertension, and protect patients from hypertension in T2DM. As an important thrifty gene, environment factors may exerts an effect of PPARγ2 on glucose homeostasis and insulin resistance.     

Relationship between Invasiveness of Pituitary Somatotrophinomas and Structural Abnormalities of Protein Kinase C Gene in Human

Morerecently,attenti0nhasbeenf0-cused0nthepotentialrolesofmembranephosphatidylinositol(PI)hydrolysisandproteinkinaseC(PKC)ins0mat0trophfunction[2-4'9].PKCactivityandexpressionwerefoundtobesignificantlyhigherinhu-manpituitarytumorscomparedwithnormaltis.ue.[3.9]-AlteredPKCstructurewasdemonstratedt0beassociatedwithinvasive-nessoftumors[4],suggestingthatthealphaisoform0fPKC(aPKC)mayharboras0-maticmutati0nincodon294,therebyre-sultinginsubstitutionofasparticacid(GAC)byglycine(GGC)inacorresp…     

Relationship between the Expression of α1-Antitrypsinase in Bronchioalveolar Carcinoma and Clinical Pathology

Bronchioalveolar carcinoma (BAC) was classified into lung adenocarcinoma in WHO types. Itwas reported that the increase in adenocarcinomawas due to an increase in BAC[1]. The histogenesisfor BAC was nuclear now. al--antitrypsinase (alAT) is an protease inhibitor mainly synthesizedand secreted by hepatocytes, which can inhibit theactivities of various protein hydrolases and limitthe growth and diffusion of tumor cells to some extent. Several studies demonstrated that al--ATcould be expres…     

Relationship between the expression of α1-antitrypsinase in bronchioalveolar carcinoma and clinical pathology

Summary By using immunohistochemistry LSAB method and imaging analysis technique, the expression of α1-antitrypsinase (α1-AT) in 41 cases of bronchioalveolar carcinoma (BAC) was quantitatively detected to explore the relationship between α1-AT expression in BAC tissues and clinical pathology. The results showed that the total positive rate for α1-AT expression was 85. 4 %. The positive rate for α1-AT expression in alveolar BAC was 100 %, with the immunity reactive staining intensity being significantly higher than in papillary BAC, mucinous BAC or sclerosing BAC (P < 0. 05). The positive rate in papillary BAC was 93. 3 %, with the intensity higher mucinous BAC or sclerosing BAC (P < 0. 01); The positive rate in both mucinous BAC and sclerosing BAC was 66.7 % (P > 0. 05); The expression intensity in lymph node metastatic group was obviously lower than that in the group without metastasis (P < 0.01); The patients with mucinous BAC were diagnosed at a younger age than those with other histologic types of BAC (P < 0.05). It was suggested that BAC cells could also produce α1-AT. Detection of α1-AT could be used as a new method to diagnose BAC and might play a role in assessing BAC metastasis.     

Association of Polymorphisms of β3-Adrenergic Receptor,Angiotensinogen and Angiotensin-Converting Enzyme Gene with Hypertension in type 2 DM

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