202例正常人与35例冠心病患者血清中高密度脂蛋白胆固醇含量的初步报告

血清中高密度脂蛋白为脂蛋白中的一种去脂蛋白质。经过实验证明,认为其在限制动脉壁胆固醇的蓄积及促进其清除上有一定的作用。近年来临床上测定血清高密度脂蛋白胆固醇(HD L—Ch)的含量,以探讨与冠心病的关系,为防治冠心病寻找新的途径。本文测定了202例正常成人血清中HDL—Ch含量。并对其中的129例测定了高密度脂蛋白胆固醇与总胆固醇比值(HDL—Ch/Ch)。同时对35例冠心病患者血清中HDL—Ch含量与HDL—Ch/Ch值进行了观察。现将结果报告如下:     

酒精所致精神障碍患者血脂水平分析

本研究对酒精所致精神障碍患者的血清总胆固醇(TCH)、甘油三酯(TG)和高密度脂蛋白胆固醇(HDL-ch)水平进行测定,现将结果报告如下.     

86名女性体检人员血脂检测结果分析

我们对86名女性的总胆固醇(CHO)、甘油三酯(TG)和高密度脂蛋白胆固醇(HDL-C)水平进行测定和分析，结果报告如下。     

脂蛋白代谢与动脉粥样硬化的关系

本文报道了一般健康人877例,运动员328例,心,脑血管病261例,和其他疾病86例的高密度脂蛋白-胆固醇(HDL-Ch),低密度脂蛋白-胆固醇(LDL-Ch)和极低密度脂蛋白-胆固醇(VDLD-Ch)含量,所得结果与国外文献报道十分接近。在防治动脉粥样硬化时LDL-Ch/HDL-Ch的测定较血脂和其他脂蛋白组分的测定更为敏感。     

载脂蛋白A_Ⅰ、B与高血压病的关系

1981年12月至1989年12月对内科住院及门诊高血压患者50例与正常对照组50例,同时测定高密度脂蛋白胆固醇(HDL-C),低密度脂蛋白胆固醇(LDL—C),极低密度脂蛋白胆固醇(VLDL-C),以及总胆固醇(TC),甘油三脂(TG),载脂蛋白(APO)A_1和B_(100),今报告如     

熔解曲线分析法检测乙醛脱氢酶2基因多态性

目的应用熔解曲线分析法建立乙醛脱氢酶2(ALDH2)基因Glu504Lys多态性分型方法并探讨其临床应用价值。方法提取受检者EDTA-K2抗凝外周血的基因组DNA,用等位基因特异性PCR(AS-PCR)扩增ALDH2基因,通过熔解曲线分析扩增峰,对乙醛脱氢酶2基因504位点的多态性进行基因分型,并与DNA微阵列芯片法的检测结果进行比较,出现结果不一致时,用基因测序法进行验证。结果对60例外周血标本进行ALDH2基因的多态性分型检测,两种方法检测结果均为野生型(Glu504Glu)27例,突变型(Lys504Lys)13例,杂合型(Glu504Lys)20例,熔解曲线分析方法与DNA微阵列芯片法分析结果完全一致(Kappa值为1)。结论本研究成功建立了基于熔解曲线分析检测乙醛脱氢酶2基因Glu504Lys多态性分型方法。     

ABCB4基因外显子6突变与妊娠期肝内胆汁淤积症的相关研究

目的:探讨ABCB4基因外显子6突变与妊娠期肝内胆汁淤积症(ICP)发病的关系。方法:抽提12例ICP病例标本外周血DNA,进行PCR扩增。对PCR产物进行DNA序列测定,以明确其具体的突变性质。结果:12例妊娠期肝内胆汁淤积症患者的血样品均扩增出ABCB4基因外显子6的靶基因片段,对12例样品的PCR产物全部测定DNA序列,发现7例标本ABCB4基因第504位碱基发生了变化,其中3例为c.504C>T杂合突变,4例为c.504C>T纯合突变。结论:本研究提示ABCB4基因外显子6突变与妊娠期肝内胆汁淤积症(ICP)的发病具有一定的相关性。     

2203名45岁以上宁波港职工体检血脂分析

<正> 为了解本港职工健康人群的血脂状况,1997年10月对我单位2203名45岁以上职工进行了血脂测定,结果及分析报告如下。 1.对象与方法 对象 2203例受检对象中有干部、技术人员、船员、工人及管理人员等。其中男1699人,女504人。年龄最小45岁,最大89岁。 方法 (1)检测方法:空腹静脉抽血,采用常规酶测定法。总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL)的正常值范围:TC 2.8～6.0mmol/L,TG<2.     

P504S/AMACR在前列腺癌患者血清中的表达及其意义

目的:研究血清P504S/AMACR在前列腺癌筛查和早期诊断中的价值.方法:应用酶联免疫吸附试验(ELISA法),测定30例未经治疗的前列腺癌和20例未经治疗的前列腺增生患者血清P504S/AMACR水平.结果:(1) 前列腺癌组血清P504S/AMACR质量浓度[(4.12±0.24)ng·m1-1]明显高于前列腺增生组[(1.52±0.47)ng·m1-1],差异具有统计学意义(P<0.01).(2) 前列腺癌患者血清P504S/AMACR质量浓度与Gleason评分无关(P>0.05).(3) 前列腺癌患者血清P504S/AMACR质量浓度与PSA质量浓度无关(P=0.392).结论:初步证实检测P504S/AMACR在前列腺癌患者血清中的水平,可辅助PSA应用于前列腺癌的筛查,提高前列腺癌的早期诊断率.P504S/AMACR有望成为有效的前列腺癌瘤标之一.     

肝硬化患者血脂水平分析

笔对我院门诊、住院的56例肝硬化患进行血清总肝固醇(CHOL)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL—C)测定，以探讨其在肝硬化患中的临床应用价值，现将结果报告如下：     

p27kip1基因在人胆管癌细胞化疗增敏效应中的作用

目的 探讨外源性p27^kip1在人胆管癌细胞系QBC939中高表达对胆管癌细胞化疗敏感性的影响。方法 通过携带人p27^kip1基因的重组腺病毒载体Ad-p27mt转染人胆管癌细胞系QBC939。经逆转录-聚合酶链反应（RT-PCR）、Western blot检测p27^kip1在胆管癌细胞中的表达；经MTT比色法及流式细胞术检测5-氟尿嘧啶（5-FU）、丝裂霉素（MMC）、环磷酰胺（CTX）对转染p27^kip1前后的QBC939细胞生长抑制及凋亡的影响。结果 Ad-p27mt转染后5-FU、MMC和CTX对QBC939细胞生长抑制率，分别由转染前的41．89％、45．59％和38．91％显著增加为56．15％、55．65％和51．69％；凋亡率也由13．76％、11．76％和10．46％，明显升高为41．39％、35．94％、34．46％，差异均有显著性意义（均P〈0．05）。结论 p27^kip1在QBC939细胞中高表达，能显著增强胆管癌细胞对化疗药物的敏感性，为基因联合化疗药物治疗胆管癌提供了参考依据。     

四种小檗胺衍生物与红细胞膜Ca~(2 )-Mg~(2 )-ATPase的相互作用

四种小檗胺衍生物对人红细胞膜Ca~(2 )-Mg~(2 )-ATPase的基础活性无影响,但对钙调素(CaM)和胰蛋白酶限制性酶解这两种形式所活化的Ca~(2 )-Mg~(2 )-ATPase都有不同程度的抑制作用,且有平行的抑制强度。初步探讨了这类化合物影响Ca~(2 )-Mg~(2 )-ATPase活力的可能机制。     

A hospital outbreak of severe acute respiratory syndrome in Guangzhou, China

Objective To describe a hospital outbreak of severe acute respiratory syndrome (SARS) and summarize its clinical features and therapeutic approaches.Methods The outbreak started with a SARS patient from the community, and a total of 96 people (76 women and 20 men, mean age (29.5±10.3) years, 93.8% of whom were health care workers) who had exposure to this source patient became infected in a short time. Clinical data in this cohort were collected prospectively as they were identified.Results (1) The incubation period ranged from 1 to 20 (mean: 5.9±3.5) days. The duration of hospitalization was （17.2±8.0） days. (2) The initial temperature was （38.3±0.6）℃, while the highest was （39.2±0.6）℃ (P&lt;0.001), with fever duration of （9.0±4.2） days. (3) Other most common symptoms included fatigue (93.8%), cough (85.4%), mild sputum production (66.7%), chills (55.2%), headache (39.6%), general malaise (35.4%) and myalgia (21.9%). (4) The radiographic changes were predominantly bilateral in the middle or lower lung zones. The number of affected lung fields was 1.2±0.8 on presentation, which increased to 2.9±1.4 after admission (P&lt;0.001). The interval from the beginning of fever to the onset of abnormal chest radiographs was （3.5±2.3） days, which increased in size, extent, and severity to the maximum （6.7±3.5） days later. The time before the lung opacities were basically absorbed was （14.9±7.8） days. (5) Leukopenia was observed in 67.7% of this cohort. The time between the onset of fever and leukopenia was （4.4±2.3） days, with the lowest white blood cell count of （2.80±0.72）×10(9)/L. (6) The lowest arterial oxygen saturation was （94.8±3.1）% with supplementary oxygen. (7) Antibiotical therapies included tetracyclines (91.0%), aminoglycosides (83.3%), quinolones (79.2%); 18.8% of the patients received a combination of tetracyclines and aminoglycosides, while 11.5% received a combination of tetracyclines and quinolones, and 63.5% received a combination of tetracyclines, aminoglycosides and quinolones. Vancomycin was used in 13.5% of the patients. (8) 68.8% of the patients were treated with methylprednisolones for a mean interval of （4.9±2.4） days. The initial dose was （67.3±28.2） mg/d and the maximal dose was （82.4±30.5） mg/d. (9) Human γ-globulin, interferon-α, antiviral drugs (oral ribavirin or oseltamivir) were used respectively in 68.6%, 46.9% and 92.7% of the patients. (10) Ninety-five patients (99.0%) had a complete clinical recovery, and only 1 patient (1.0%) died.Conclusions SARS appears to be quickly infectious and potentially lethal among health care workers, characterized by acute onset and rapid progression, and mostly bilateral lung involvement on chest radiographs. Proper administration of glucocorticosteroids seems to be of some benefits. Antibiotics, human γ-globulin, interferon-α, and antiviral drugs, although empirically, might be useful to shorten the clinical course. Chin Med J 2003; 116(6):811-818     

Associations of blood pressure and arterial compliance with occupational noise exposure in female workers of textile mill

Background There are few studies on the arterial compliance of noise exposure. The purpose of this study was to understand the relationship between hearing loss, blood pressure and arterial compliance of female workers who exposed to occupational noise in a textile mill.Methods The noise levels in the workplace were measured with a HS6288 sound level meter. Cumulated noise exposure (CNE) was calculated according to the noise intensity and the exposure period. Hearing ability and arterial compliance were measured in 618 noise exposed workers. The database was set up with EpiData and the statistical analysis was performed with SAS software 9.1.3. Results The noise levels were 80.1dB(A) to 113.5dB(A), of which the levels at 92.5% of the noise monitoring sites were over the national standard. The incidence of high frequency hearing loss (HFHL) was 24.43% and language frequency hearing impairment (LFHI) was 0.81%. The incidence of hypertension was 7.93%. Both systolic blood pressure (SBP) and diastolic blood pressure (DBP) in the high frequency hearing loss group were significantly higher than those in the normal hearing group (P&lt;0.05), while C(1) (large artery compliance) and C(2) (small artery compliance) were significantly lower (P&lt;0.05). The high frequency hearing threshold (HFHT) of the hypertension group was significantly higher than in the normal blood pressure group (P&lt;0.05), while C(1) and C(2) were significantly lower (P&lt;0.05). C(1) and C(2) had a negative correlation with HFHT, SBP, DBP, mean of arterial pressure (MAP), pulse pressure (PP) and pulse rate (PR) (P&lt;0.05). The multiple regression analyses showed that blood pressure and PR were the main influencing factors on C(1) and C(2). LFHT was an influence on C(2) and HFHT on C(1).Conclusions Textile mill noise pollution is very serious and has an obvious influence on worker’s auditory function. The female workers with low artery compliance or with high blood pressure might be suffering from hearing loss; those with noise induced hearing loss might be suffering from hypertension if she is constantly exposed to loud industrial noise.     

P53和C-erbB-2在乳腺癌中的表达及意义

目的 分析研究P^53和C—erbB-2在乳腺癌中的表达与转移及预后的关系。方法 免疫组化法测定93例原发性乳腺癌中P^53和C—erbB-2表达情况。结果 P^53和C—erbB-2在乳腺癌中阳性表达率分别为55.9％（52／93）和40.9％（38／93），其阳性表达率随着临床分期增加和腋窝淋巴结转移数目的增加而增加，差异有显著性（P〈0．05）；P^53和C-erbB-2共同阳性表达与淋巴结转移差异有显著性（P〈0．05）。结论 P^53、C—erbB-2是判断乳腺癌预后的有效指标，联合检测P^53和C—erbB-2可指导临床综合治疗。     

捐精志愿者支原体感染后用药对精液质量的影响

目的 评估支原体感染后用药治疗对精液质量的影响。方法统计2016年1月至2019年12月在广东省人类精子库支原体筛选检查阳性的445例志愿者经口服药物治疗前后的精液分析资料,志愿者连续用药2周,停药1周及2个月后再次进行精液检查。比较志愿者用药前、用药后1周、用药后2个月精液质量的变化。结果用药前、用药后1周、用药后2个月精液量分别为(3.85±1.37)mL、(3.81±1.48)mL、(3.79±1.37)mL,差异无统计学意义（P=0.362）;用药前后精子浓度分别为(96.98±35.35)×106·mL-1、(90.18±40.68)×106·mL-1、(88.57±34.44)×106·mL-1,用药后精子浓度降低,差异有统计学意义（P=0.008）;用药前后前向运动精子百分比分别为(60.52±9.45)%、(58.02±12.23)%、(59.41±11.15)%,用药后前向运动精子百分比降低,差异有统计学意义（P=0.004）;用药前后非前向运动精子百分比分别为(3.19±1.79)%、(3.31±2.02)%、(3.38±2.19)%,差异无统计学意义（P=0.904）;用药前后不动精子百分比分别为(36.28±9.22)%、(38.68±12.10)%、(37.20±11.07)%,用药后不动精子百分比升高,差异有统计学意义（P=0.004）;用药前后总精子数分别为(365.06±167.44)×106、(335.77±184.76)×106、(324.47±146.87)×106,用药后总精子数降低,差异有统计学意义（P=0.003）;用药前后前向运动精子总数分别为(220.76±105.31)×106、(194.68±112.45)×106、(193.27±93.29)×106,用药后前向运动精子总数降低,差异有统计学意义（P=0.039）。结论支原体用药治疗对精液质量有较大影响,主要体现在降低精子浓度和精子活力,使不动精子占比增加,对精液量没有显著影响;总精子数和前向运动总精子数显著降低。     

乳腺癌雌激素受体与超微结构的关系

应用免疫金银染色技术对23例乳腺癌组织进行雌激素受体(ER)检测,探讨ER与乳腺癌超微结构的关系.ER阳性率为16/23.ER阳性组乳腺癌细胞较多见有核内小体.癌细胞微绒毛在ER阳性组较阴性组既长且宽(P<0.05);ER染色强度与微绒毛长度呈正比(P=0.0004).ER阳性、阴性组间及不同阳性程度间微绒毛长/横径比无差别(P>0.05).ER染色程度强者粗面内质网、线粒体体密度及粗面内质网面密度较大(P<0.05).ER阳性组较阴性组粗面内质网体密度大(P=0.0145).     

不同实验条件对昆明和BALB/C小鼠强迫游泳实验的影响

目的 研究不同实验条件对小鼠强迫游泳实验的影响,为研究小鼠抑郁模型的敏感性、有效性、可靠性提供可行的实验参考标准.方法 将不同性别,体质量相近的昆明小鼠和BALB/C小鼠,放置在相同的饲养、实验环境下,观察在不同水温、昼夜节律改变的环境下,不同品系间、同品系雌雄间小鼠强迫游泳行为的变化.结果 ①不同品系的小鼠在昼夜节律变化的强迫游泳试验中,白天不动时间有统计学差异,昆明小鼠的不动时间[(91.95±40.32)s]明显少于BALB/C小鼠的不动时间[(142.42±33.58)s];BALB/C小鼠白天的不动时间[(142.42±33.58)s]明显长于夜间的不动时间[(104.89±34.33)s].②BALB/C小鼠的阳性结果数明显多于昆明小鼠;同品系雌雄小鼠间的不动时间相差不大,但是雄性小鼠的阳性结果数明显多于雌性小鼠.③BALB/C小鼠在22C水温的不动时间[(92.24±25.81)s]明显长于其在32℃水温的不动时间[(60.72±11.11)s].结论 为增加强迫游泳实验的敏感性、可靠性应选择雄性小鼠宜在白天进行实验;BALB/C小鼠相对于昆明小鼠及其在22℃水温中更容易产生抑郁样行为.

Abstract：

Objective To investigate the sensitivity and variability factors that were assessed on the forced swimming test (FST) using BALB/C and Kunming mice. Methods The immobility time of FST was compared using Kunming and BALB/C mice in different experimental conditions including circadian rhythm ( day and night) ,gender and water temperature ( 12,22 and 32℃ ) . Results (①) The immobility time of BALB/C during the daytime( ( 142.42 ± 33.58) s) was significantly increased than that at night ( ( 104.89 ± 34.33 ) s). (② The immobility time of Kunming mice( (91.95 ± 40.32) s) was significantly decreased than that of BALB/C mice ( ( 142.42 ± 33.58 ) s). (③)The immobility time under the water temperature of 22 C ( ( 92.24 ± 25.81 ) s) was significant longer than that under the water temperature of 32C ( (60.72 ± 11.11 ) s). Conclusion BALB/C stain,male mice,daytime and water temperature of 22℃ should be chosen in the FST.