麦胚降血脂干预试验

社区高危人群经筛检高胆固醇血症149人，随机分麦胚粉（Ⅰ）、对照（Ⅱ）、烟酸肌醇酯（Ⅲ）、3组；组具有可比性，随访依从率90％，原则 上遵照盲法观察。结果表麦胚TC作用Ⅰ组优于Ⅲ组（F＝2．87、P＝0．06），Ⅰ组LDL－C显著低于Ⅲ组（F＝4．26、P＝0．016），并有提高HDL2－C作用，服用麦胚粉无异常反应。麦胚含有较丰富的不饱和脂肪酸和有利于心血管的微量元素，可用于人群CVD的一级预防。     

广州市荔湾区2014年健康人群脊髓灰质炎抗体水平分析

目的了解广州市荔湾区健康人群脊髓灰质炎(简称脊灰)中和抗体水平,为制定和调整免疫策略提供依据。方法在广州市荔湾区随机抽取3家医院,随机抽取6月龄以上健康人员106人,采集其静脉血以微量细胞中和抗体试验法测定脊灰中和抗体,建立Excel数据库,用SPSS 13.0软件进行统计分析。结果共抽取106人,脊灰Ⅰ、Ⅱ、Ⅲ型中和抗体阳性率分别为98.11%、89.62%、95.28%;几何平均滴度(geometric mean titer,GMT)分别为1∶172.67、1∶68.67、1∶171.47,各型间GMT差异无统计学意义(F=0.588,P>0.01)。不同性别人群的脊灰Ⅰ、Ⅱ、Ⅲ型病毒的抗体阳性率差异无统计学意义(χ2=12.59,P>0.05),不同年龄组人群的脊灰不同型病毒的抗体GMT之间比较差异均无统计学意义(Ⅰ型与Ⅱ型比较、Ⅰ型与Ⅲ型比较、Ⅱ型与Ⅲ型比较,F值分别为0.656、0.562、0.656,P>0.01),不同户籍人群的脊灰Ⅰ、Ⅱ、Ⅲ型病毒的抗体阳性率差异无统计学意义(χ2=0.002、0.009、1.138,P>0.05)。不同户籍人群的脊灰不同型病毒的抗体GMT之间比较中,Ⅱ型与Ⅲ型比较差异有统计学意义(F=6 396.89,P<0.01),Ⅰ型与Ⅱ型、Ⅰ型与Ⅲ型比较差异均无统计学意义(F=10.99、0.06,P>0.01)。结论广州市荔湾区健康人群脊灰抗体水平较高,对脊灰形成了免疫屏障。     

手足口病病例早期血糖监测及干预的临床研究

目的:对早期血糖监测在手足口病病例中的价值进行探讨。方法:选取本院收治的150例手足口病患儿,按照病情严重程度分组为Ⅰ期和Ⅱ期(及时就诊)及Ⅲ期组(未及时就诊),每组50例,比较三组患儿的血糖、白细胞监测结果。结果:入院初期,Ⅲ期组患儿血糖水平均明显高于Ⅰ、Ⅱ期组(P0.05),Ⅰ期与Ⅱ期组比较差异无统计学意义(P0.05)。实施及时血糖监测、控制和相应治疗后,Ⅰ、Ⅱ期组患儿的临床治疗总有效率明显高于Ⅲ期组患儿(F=16.97,P0.05),住院时间和后遗症发生率低于Ⅲ期组患儿(P0.05)。患儿的血糖水平也明显降低。结论:对手足口病患儿早期进行血糖监测、及时实施干预,可有效阻止患儿病情进展。     

大鼠孕期和哺乳期高蛋白饮食对子代代谢相关激素的影响

目的：探讨母体孕期和哺乳期高蛋白饮食对子代代谢相关激素的影响。方法：雌性Wistar大鼠交配成功后(F0代),随机分为3组：ProⅠ组、ProⅡ组、ProⅢ组,分别喂以不同蛋白含量的等热卡纯化饲料：DietⅠ(蛋白质：14%;碳水化合物：69.3%,E/E)、DietⅡ(蛋白质：24%;碳水化合物：59.3%;E/E)、DietⅢ(蛋白质：34%;碳水化合物：49.3%,E/E),相应饮食贯穿孕期和哺乳期。子代(F1代)出生后3 d(P3)剔除雌性并小窝喂养,于P21断乳后均以正常标准饲料喂养至实验结束(P77)。期间采集子代P21、P49、P77血样,并检测其代谢相关激素的水平。结果：P21时点,ProⅠ组F1代血清胰岛素样生长因子(IGF-1)水平低于ProⅡ组(P＜0.01);至P49时点,ProⅡ组、ProⅢ组IGF-1水平均高于ProⅠ组(P＜0.01);至P77时点,ProⅢ组IGF-1水平为3组中最高(P＜0.01)。P21时点,ProⅡ组F1代血清瘦素(Leptin)水平为3组中最高(P＜0.01);P49和P77时点,ProⅠ组Leptin水平持续低于ProⅡ组和ProⅢ组(P＜0.05)。ProⅢ组F1代P49时点血浆神经肽Y(NPY)水平低于ProⅠ组(P＜0.01),P77时点各组无差异(P＞0.05)。ProⅢ组P77时点胃促生长素(Ghrelin)水平高于ProⅠ组和ProⅡ组(P＜0.01),P49时点各组无差异(P＞0.05)。结论：大鼠孕期和哺乳期高蛋白饮食可引起其子代外周血中Leptin、Ghrelin、IGF-1水平升高,对外周血中NPY浓度影响较小。     

利用锥形束CT对不同磨牙关系患者下颌第一磨牙咬合接触情况的研究

目的:利用锥形束CT分析不同磨牙关系患者下颌第一磨牙咬合接触情况,并探索该方法的可行性。方法:选取2013年1月至2013年6月于我院放射科就诊需行锥形束CT检查的患者,按照纳入排除标准遴选出347例患者,按照Ⅰ、Ⅱ、Ⅲ类磨牙关系分别记录患者下颌第一磨牙面上近中、中、远中3部分的A、B、C咬合接触点分布情况,并利用SAS 9.2软件进行统计分析。结果:3类接触点总数比较,Ⅰ类130例(26.62±10.79)、Ⅱ类131例(26.75±10.81)、Ⅲ类86例(19.16±9.14),差异有统计学意义(H=28.346,P=0.000),组间比较Ⅲ类接触点较其余2类均少,差异有统计学意义(P<0.016 7)。近中接触点数目比较,Ⅰ类(5.62±3.56)、Ⅱ类(7.96±4.06)、Ⅲ类(4.47±3.61),差异有统计学意义(H=45.045,P=0.000),组间两两比较差异有统计学意义(P<0.016 7);中部接触点数目比较,Ⅰ类(10.3±4.74)、Ⅱ类(9.24±4.54)、Ⅲ类(6.53±4.04),差异有统计学意义(H=32.390,P=0.000),组间比较Ⅲ类最少,余无统计学差异;远中接触点数目比较,Ⅰ类(10.7±4.89)、Ⅱ类(9.55±5.35)、Ⅲ类(8.16±4.72),差异有统计学意义(H=12.025,P=0.002),其中Ⅲ类数目较Ⅰ类少(P<0.0167),余无统计学差异。A点数目比较,Ⅰ类(10.46±5.75)、Ⅱ类(9.53±5.2)、Ⅲ类(7.83±5.02),差异有统计学意义(H=8.466,P=0.015),组间比较Ⅲ类较Ⅰ类少(P<0.016 7),余无统计学差异;B点数目比较,Ⅰ类(9.66±5.58)、Ⅱ类(10.87±5.08)、Ⅲ类(6.17±4.7),差异有统计学意义(H=39.598,P=0.000),组间比较Ⅲ类数目最少(P<0.016 7),余无统计学差异;C点数目比较,Ⅰ类(6.49±3.46)、Ⅱ类(6.34±4.63)、Ⅲ类(5.16±3.39),差异有统计学意义(H=7.179,P=0.028),组间比较Ⅲ类较Ⅰ类少(P<0.016 7),余无统计学差异。结论:Ⅲ类磨牙关系患者三点式咬合接触点总数、3个部位及B点数目均较其他2类少,牙尖交错位时咬合接触稳定性差;锥形束CT可作为一种新方法分析咬合接触情况。     

外科治疗DeBakeyⅠ型和Ⅲ型夹层合并胸腹主动脉瘤的结果对比

目的对比分析外科治疗DeBakeyⅠ型夹层和Ⅲ型夹层合并胸腹主动脉瘤(TAAA)的早期和中期结果。方法回顾性分析2009年1月至2017年12月在阜外医院接受手术治疗的130例主动脉夹层合并胸腹主动脉瘤患者的临床资料,其中DeBakeyⅠ型患者47例(Ⅰ型组),DeBakeyⅢ型患者83例(Ⅲ型组),比较两组患者手术后的早期结果、中期生存和再干预情况。结果术后30 d,全组死亡9例(6.9%),其中Ⅰ型组5例(10.6%),Ⅲ型组4例(4.8%),差异无统计学意义(χ²=0.803,P=0.370)。Ⅰ型组和Ⅲ型组术后早期主要不良事件发生率(38.3%比51.8%;χ²=2.199,P=0.138)、5年生存率[(81.7±5.9)%比(87.2±4.2)%;χ²=0.483,P=0.487]和5年免于再干预结果[(84.5±6.7)%比(85.5±4.8)%;χ²=0.010,P=0.920]差异均无统计学意义。结论本组外科治疗DeBakeyⅠ型和Ⅲ型夹层合并TAAA的早期和中期结果相似,今后需进...     

TGF-β1、CTGF对判断慢性阻塞性肺疾病合并纤维化的临床意义

目的 探讨TGF-β 1、CTGF对判断慢性阻塞性肺疾病合并纤维化的临床意义.方法 选择COPD112例,根据是否合并PF分为COPD组(A组)、COPD合并PF组(B组),并选择健康人群50例为对照组(C组),分别有78、34、50例,分别对各组TGF-β1、CTGF、PⅠ NP、PⅢNP进行检测.结果 A组患者TGF-β1、CTGF水平较C组均出现显著性升高(P＜0.05),B组患者较A、C组TGF-β1、CTGF均出现显著升高(P＜0.05).A组患者PⅠ NP、PⅢNP及PⅠ NP/PⅢNP较C组均出现显著升高(P＜0.05),B组患者PⅠ NP、PⅢNP及PⅠ NP/PⅢNP较A组及C组均出现显著升高(P＜0.05).TGF-β1与PⅠ NP、PⅠ NP/PⅢNP显著正相关(P＜0.05),CTGF与PⅠ NP、PⅢNP、PⅠ NP/PⅢNP正相关(P＜0.05).结论 TGF-β1、CTGF水平升高与COPD患者肺纤维化指标密切相关,对其水平检测有助于明确肺纤维化的进展.     

异丙酚对低温体外循环期间脑氧代谢的影响

①目的　观察不同剂量异丙酚对低温体外循环 (CPB)期间颈内静脉球血氧饱和度 (SjO2 )、脑氧摄取率 (CEO2 )、脑动脉颈内静脉血氧含量差 (Ca jO2 )的影响。②方法　 33例心脏瓣膜置换术病人 ,随机分成 3组 ,Ⅰ组静注异丙酚 5mg·kg- 1 ·h- 1 ;Ⅱ组静注异丙酚 2mg·kg- 1 ·h- 1 ;Ⅲ组 (对照组 )不静注异丙酚。在CPB前 (T1 )、降温至 33℃ (T2 )、低温稳定期 (T3)、复温至 33℃ (T4 )以及CPB结束后 2 0min(T5)测定SjO2 、CEO2 、Ca jO2 。③结果　Ⅰ、Ⅱ组T2 ～T5时 ,Ⅲ组T2 、T3时SjO2 明显高于T1 时 (F =6 .2 4～ 1 0 .50 ,q =3 .59～ 7.59,P <0 .0 5) ;Ⅲ组T4 、T5时SjO2 明显低于T3时 (q =4.1 4、4 .69,P <0 .0 5) ;Ⅱ组T4 时SjO2 明显高于Ⅲ组 (F =4.1 8,q =4.0 8,P <0 .0 5)。Ⅰ、Ⅱ组T2 ～T5时 ,Ⅲ组T2 、T3时CEO2 均比T1 时下降 (F =4.1 5～ 8.70 ,q =4.98～8.2 0 ,P <0 .0 5) ;Ⅲ组T4 、T5时CEO2 明显高于T3时 (q=4.30、6 .35 ,P <0 .0 5) ;Ⅱ组T4 时CEO2 明显低于Ⅲ组 (F =4.41 ,q =4.48,P <0 .0 5)。Ⅱ组T2 ～T5时 ,Ⅲ组T2 ～T4 时Ca jO2 均较T1 时明显下降 (F =6 .30～ 1 0 .50 ,q =5 .0 5～ 8.60 ,P <0 .0 5) ;Ⅲ组T4 、T5时Ca jO2 明显高于T3时 (q =4.30、1 3 .70 ,P <0 .0 5) ;Ⅲ组T4 时C     

腺苷预处理对大鼠局灶性脑缺血再灌注后线粒体活性氧含量及线粒体呼吸链复合体Ⅰ、Ⅲ活性和脑组织总抗氧化能力的影响

目的观察腺苷预处理对大鼠局灶性脑缺血再灌注后线粒体活性氧(ROS)含量及线粒体呼吸链复合体Ⅰ、Ⅲ活性和脑组织总抗氧化能力(TAC)的影响。方法将18只雄性Sprague Dawley大鼠随机分为假手术组、缺血再灌注组和腺苷预处理组,每组6只。造模前3 d腺苷预处理组大鼠腹腔注射腺苷注射液(1.5 mg·kg~(-1)),每日1次;假手术组和缺血再灌注组大鼠在相同时间点腹腔注射2 m L生理盐水。缺血再灌注组和腺苷预处理组大鼠采用改良线栓法制作大脑中动脉闭塞(MCAO)模型,假手术组大鼠术中只分离血管,不插入线栓。血流阻断2 h后将线栓拔出,形成再灌注。造模后24 h采用化学荧光法检测3组大鼠缺血区脑组织线粒体中ROS水平及线粒体呼吸链复合体Ⅰ、Ⅲ活性和TAC。结果缺血再灌注组大鼠缺血区脑组织线粒体中ROS水平显著高于假手术组(t=5.122,P=0.000),腺苷预处理组大鼠缺血区脑组织线粒体中ROS水平显著低于缺血再灌注组(t=4.107,P=0.001)。缺血再灌注组大鼠缺血区脑组织线粒体呼吸链复合体Ⅰ、Ⅲ活性显著低于假手术组(t=3.632、3.472,P=0.002、0.003),腺苷预处理组大鼠缺血区脑组织线粒体呼吸链复合体Ⅰ、Ⅲ活性显著高于缺血再灌注组(t=2.623、2.193,P=0.019、0.045)。3组大鼠缺血区脑组织TAC比较差异均无统计学意义(F=1.755,P=0.207)。结论腺苷预处理可通过保护线粒体呼吸链复合体Ⅰ、Ⅲ活性而减少MCAO大鼠脑缺血再灌注后线粒体ROS的生成。     

不同肾造瘘管及固定方式对肾结石合并肾功能不全患者术后并发症的影响

目的:探讨不同直径的肾造瘘管及固定方式对肾结石合并肾功能不全患者经皮肾镜碎石术后并发症的影响。方法:采用随机研究方法,根据造瘘管直径和固定方式不同随机分为3组:Ⅰ组,14 Fr球囊硅胶引流管(Uro-vision)牵拉组;Ⅱ组,14 Fr球囊硅胶引流管(Urovision)不牵拉组;Ⅲ组,20 Fr无球囊乳胶引流管不牵拉组。对所有患者术前检测血清肌酐、血红蛋白、中段尿培养结果、术侧结石体积,术中记录手术时间、穿刺通道数目,计算术后24 h、72 h血红蛋白变化值,记录术后是否出现尿外渗、全身炎症反应综合征、应用毒麻药和输血情况。结果:共入选72例肾功能不全患者,每组24例。3组间在尿外渗(P=0.301)、全身炎症反应综合征(P=0.099)及应用毒麻药方面差异无统计学意义(P=0.898)。在术后24 h和72 h Hb变化值方面,Ⅰ组与Ⅱ组(P=0.001,P=0.009)、Ⅰ组与Ⅲ组(P=0.021,P=0.003)之间差异有统计学意义,Ⅱ组与Ⅲ组之间差异无统计学意义(P=0.989,P=0.962)。3组术后输血例数由低到高依次为:Ⅰ组(1例)、Ⅲ组(6例)、Ⅱ组(10例),Ⅰ组明显低于Ⅱ组(P=0.002),Ⅲ组与Ⅰ组(P=0.102)、Ⅲ组与Ⅱ组(P=0.221)之间差异无统计学意义。结论:留置14 Fr球囊硅胶肾造瘘管并牵拉压迫穿刺通道的引流方式,有利于减少结石合并肾功能不全患者术后24 h及72 h的出血量,值得推荐。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.