泰山散瘀膏抗炎镇痛作用的实验研究

目的：研究泰山散瘀膏的抗炎镇痛作用.方法：取小鼠32 只,随机分为致炎液实验组及致炎液对照组、冰醋酸实验组及冰醋酸对照组,每组8 只,利用致炎液建小鼠耳廓肿胀模型,观察泰山散瘀膏的抗炎作用;采用小鼠醋酸扭体法研究泰山散瘀膏的镇痛作用.结果：泰山散瘀膏能显著降低小鼠耳廓肿胀程度,显著抑制冰醋酸所知疼痛后的扭体次数.结论：泰山散瘀膏具有明显的抗炎阵痛作用.     

沐浴藏盐对动物抗炎、镇痛作用的初步探讨

[目的]研究沐浴藏盐对动物的抗炎、镇痛作用,便于将其开发利用,以达到强身健体,特别是用于妇女病的防治和治疗。[方法]抗炎作用用皮肤毛细血管通透性亢进法和腹腔毛细血管通透性亢进法。镇痛作用用扭体法和电击法。[结果]毛细血管通透性亢进试验,以皮肤兰斑色泽深浅比较,3个试验组数值明显低于生理盐水对照组,表明本品有明显抑制小鼠腹部毛细血管通透性亢进作用;小鼠腹腔毛细血管通透试验结果,各试验组腹腔洗涤液兰色均比对照组浅,数值均低于对照组。表明“藏浴宝”有明显抑制H^＋所致小鼠腹腔毛细血管通透性亢进作用;扭体试验、电击试验、祛风镇痛百分率分别为65%、60%。[结论]“藏浴宝”有较好的抗炎、祛风镇痛作用。     

两组中药“药对”对小鼠镇痛及抗应激作用的影响

目的研究黄芪、麦冬与五味子、麦冬两组药对镇痛、抗应激的作用。方法采用热板法和扭体实验观察小鼠的镇痛作用;采用小鼠游泳、常压下耐缺氧实验观察对小鼠的抗应激作用。结果黄芪、麦冬水煎液和五味子、麦冬水煎液能明显减少小鼠扭体次数(P﹤0.05);黄芪、麦冬水煎液能显著提高小鼠的耐热能力(P﹤0.05);两组药物均能延长小鼠耐缺氧时间(P﹤0.05)并显著延长小鼠游泳时的存活时间(P﹤0.05)。结论黄芪、麦冬与五味子、麦冬两组药对具有镇痛、抗应激的作用。     

抗炎镇痛作用中药在治疗类风湿关节炎中的应用进展

RA属中医＂痹证＂、＂顽痹＂、＂旭痹＂等范畴,西药可有效控制病情,但长期使用有一定不良反应,中医治疗RA有较好疗效,同时具有抗炎、镇痛、免疫调节等作用,且不良反应较小,本文将近些年来抗炎镇痛作用中药在治疗RA方面的研究进展作一综述。     

小茴香茎叶水提液的抗炎镇痛作用研究

目的:评价小茴香水提液的抗炎、镇痛作用.方法:应用二甲苯致小鼠耳廓肿胀研究小茴香水提液的抗炎作用,采用热板法、甲醛法、醋酸致小鼠扭体反应研究小茴香水提液的镇痛作用.结果:小茴香水提液提取物各剂量组与模型组比较,能降低二甲苯所致小鼠的耳廓肿胀度(P＜0.05);对热痛刺激的反应时间得到延缓(P＜0.05);对甲醛所致的疼...     

虫草花提取物抗炎镇痛作用研究

目的：探究虫草花是否甘具有抗炎镇痛作用，为临床的炎症、疼痛提供新的治疗方法。方法：通过小鼠给药前后热板试验测定其疼痛阈值，并通过扭体镇痛实验与耳足肿胀实验测定受试样品的抗炎镇痛作用。结果：在热板试验和扭体阵痛试验中，虫草花水提物对疼痛并无缓解作用。在足肿胀和耳肿胀试验中，虫苹花水提物具有较好的抗炎作用。结论：虫草花水提物具有与地塞米松相当的抗炎作用，且副作用小。     

通经草的镇痛作用及体外抑菌试验研究

目的研究通经草对小鼠的镇痛作用及体外抑菌作用,为发掘其药用价值提供理论依据。方法采用小鼠醋酸扭体法观察通经草水煎液的镇痛作用,选用3种不同浓度的通经草水煎液以纸片法对大肠杆菌和金黄色葡萄球菌进行体外抑菌试验。结果通经草水煎液对于小鼠镇痛作用不明显;不同浓度的通经草水煎液对金黄色葡萄球菌和大肠杆菌均具有抑制作用。结论通经草水煎液具有一定的抑菌作用,但其镇痛作用不明显。     

渭良伤科散超微粉巴布膏的抗炎镇痛实验研究

目的探讨渭良伤科散超微粉巴布膏的抗炎和镇痛作用。方法通过热板法和冰醋酸致痛试验、角叉菜胶致大鼠足趾肿胀试验、二甲苯致小鼠耳廓肿胀试验,对渭良伤科散超微粉巴布膏进行镇痛抗炎方面的药效学评价。结果渭良伤科散超微粉巴布膏中、高剂量的剂型可有效提高小鼠痛阈值,减少小鼠扭体反应次数,抑制角叉菜胶所致的大鼠足趾肿胀和抑制二甲苯所致的小鼠耳廓肿胀。结论渭良伤科散超微粉巴布膏中、高剂量的剂型有较好的镇痛抗炎作用,且在方便使用的基础上,优于原剂型渭良伤科散的镇痛抗炎作用。     

渭良伤科散超微粉巴布膏的抗炎镇痛实验研究

目的探讨渭良伤科散超微粉巴布膏的抗炎和镇痛作用。方法通过热板法和冰醋酸致痛试验、角叉菜胶致大鼠足趾肿胀试验、二甲苯致小鼠耳廓肿胀试验,对渭良伤科散超微粉巴布膏进行镇痛抗炎方面的药效学评价。结果渭良伤科散超微粉巴布膏中、高剂量的剂型可有效提高小鼠痛阈值,减少小鼠扭体反应次数,抑制角叉菜胶所致的大鼠足趾肿胀和抑制二甲苯所致的小鼠耳廓肿胀。结论渭良伤科散超微粉巴布膏中、高剂量的剂型有较好的镇痛抗炎作用,且在方便使用的基础上,优于原剂型渭良伤科散的镇痛抗炎作用。     

布洛芬,解热镇痛抗炎多面手

布洛芬是我们生活中最常用的非处方药物之一,有个头痛脑热或者牙痛、关节痛等痛疼,吃一片布洛芬就能立竿见影。但是不是每个人都可以选择布洛芬?是否可以常年服用?儿童退烧时能否一天内多次使用?针对这些疑问,有必要对布洛芬的正确使用进行解读。     

康妇炎胶囊抗炎镇痛的药效学研究

目的研究康妇炎胶囊的抗炎、镇痛作用。方法通过二甲苯致小鼠耳廓肿胀、醋酸致小鼠腹腔毛细血管通透性和琼脂致小鼠皮下肉芽肿实验观察康妇炎胶囊的抗炎作用,采用热板法和扭体法观察其镇痛作用。结果康妇炎胶囊能够显著抑制二甲苯所致小鼠耳廓肿胀,显著降低小鼠腹腔毛细血管通透性,抑制小鼠皮下肉芽肿的形成;并明显降低热刺激所致的小鼠痛阈值,减少小鼠的扭体次数。结论康妇炎胶囊对炎症有明显的抑制作用,且有一定的镇痛效果。     

Preliminary evaluation on anti-inflammatory and analgesic effects of Sideritis syriaca L. herba extracts

We studied the anti-inflammatory and analgesic activity of extracts of Sideritis syriaca L. herba. Samples were collected from the wild plant, dried, powdered, and extracted with hexane and methanol. The extracts were evaporated to dryness and then suspended in suitable solvent. They were then tested for anti-inflammatory activity in the carrageenin rat paw edema test and for analgesic activity in the Randall and Selitto test and in the tail-flick test. At 24 hours after the treatment, the gastric mucosa of each rat was observed macroscopically. Based on these results the hexane extract was fractionated by column chromatography, and the fractions were obtained tested in the same way. Results showed interesting anti-inflammatory activity only for the hexane extract and all the fractions obtained from it. All the extracts and all the other fractions showed both peripheral and central analgesic activity. In rats treated with the tested compounds hyperemia and ulcers were absent. The data from this preliminary study reveal interesting pharmacological properties of S. syriaca L. herba extracts related to the marked analgesic activity and the absence of gastric ulcerogenic activity. The same is for anti-inflammatory activity, but in this case it seems to be related only to the apolar fraction.     

补肾助孕汤对小鼠卵泡发育和子宫内膜容受性影响的实验研究

目的:研究中药补肾助孕汤对人子宫内膜基质细胞体外培养影响。方法:将中药方剂补肾助孕汤制成培养基,体外培养子宫内膜基质细胞,观察其成长状况及白血病抑制因子(leukaemia inhibitory factor,LIF)、表皮生长因子(epidermalgrowth factor,EGF)、降钙素(calcitionin,CT)的表达。结果:与对照组相比,补肾助孕汤可以促进子宫内膜细胞在体外增殖,免疫组化显示LIF(192.903 1±14.534 2 vs 183.156 8±9.189 1,P<0.001,t=5.35)、EGF(196.479 8±2.907 1 vs 184.506 4±3.733 5,P<0.001,t=26.1)、CT(183.185 6±8.422 7 vs 176.418 4±0.448 0,P<0.005,t=7.45)因子在子宫内膜基质细胞的表达相对于对照组增强。结论:中药方剂补肾助孕汤可以促进子宫内膜细胞在体外增殖,增强LIF、EGF、CT等因子的表达。     

参七汤对小鼠肺癌组织基质金属蛋白酶9表达影响的实验研究

目的研究中药参七汤对肺癌小鼠肺组织中基质金属蛋白酶9（MMP-9）表达的影响,初步探讨其预防肿瘤复发及转移的作用机制。方法T739近交系小鼠60只,按照体重相近配对原则,分为西药化疗组、中药干预组及荷瘤对照组,每组各20只小鼠。西药化疗组给予腹腔注射环磷酰胺溶液,连续5d;中药干预组给予参七汤灌胃,连续21d;各组均于造模后第22d处死小鼠,用免疫组化法观察各组小鼠肺组织MMP-9表达的变化。结果参七汤可以改善肺癌小鼠的全身一般情况,MMP-9阳性表达在西药化疗组（阳性指数3级以上者15%）及中药干预组（阳性指数3级以上者30%）均显著低于荷瘤对照组（阳性指数3级以上者75%）,差异具有统计学意义（P〈0.05）。结论中药参七汤可显著下调肺癌小鼠肺组织MMP-9的表达,推测参七汤可预防肿瘤的复发及转移。     

参七汤对小鼠肺癌组织基质金属蛋白酶9表达影响的实验研究

目的 研究中药参七汤对肺癌小鼠肺组织中基质金属蛋白酶9(MMP-9)表达的影响,初步探讨其预防肿瘤复发及转移的作用机制.方法 T739近交系小鼠60只,按照体重相近配对原则,分为西药化疗组、中药干预组及荷瘤对照组,每组各20只小鼠.西药化疗组给予腹腔注射环磷酰胺溶液,连续5 d;中药干预组给予参七汤灌胃,连续21 d;各组均于造模后第22 d处死小鼠.用免疫组化法观察各组小鼠肺组织MMP-9表达的变化.结果 参七汤可以改善肺癌小鼠的全身一般情况,MMP-9阳性表达在西药化疗组(阳性指数3级以上者15%)及中药干预组(阳性指数3级以上者30%)均显著低于荷瘤对照组(阳性指数3级以上者75%),差异具有统计学意义(P<0.05).结论 中药参七汤可显著下调肺癌小鼠肺组织MMP-9的表达,推测参七汤可预防肿瘤的复发及转移.     

五氯酚的类雌激素效应初探

目的 研究五氯酚(PCP)的类雌激素活力对MCF-7细胞增殖的影响.方法 采用离体MCF-7细胞增殖试验检测PCP的类雌激素活力,并用生长曲线对其作用机制进行初步探讨.结果 PCP在7.5×10-7～7.5×10-5 mol/L有明显刺激MCF-7细胞增殖的类雌激素活力,在7.5×10-6 mol/L增殖效应最大.细胞周期分析PCP组细胞增殖指教均明显高于溶剂对照组.结论 PCP在7.5×10-7 ～7.5×10-5 mol/L具有刺激MCF-7细胞增殖的类雌激素活力.     

Reproductive inhibition caused by the anti-inflammatory analgesic benzydamine in the rat

The anti-inflammatory analgesic benzydamine was given to pregnant rats from Day 15 of gestation to Day 21 of lactation and from Days 6 to 15 of gestation at daily oral doses of 50, 100, or 200 mg/kg. The dams were observed to assess maternal toxicity, viability, growth, and symptomatology throughout the two studies. The offspring from the first study were observed for litter size, weight, still births, and subsequent development from birth to weaning. In the second study, all dams were sacrificed on Day 20 and examined for parameters of fecundity, embryotoxicity, and teratogenicity. Dams given 200 mg benzydamine/kg during late gestation and lactation exhibited signs of maternal toxicity and a 35% mortality. Significant decreases in litter size, weight, live births of either sex, and progeny body weights were noted at birth from these dams. Subsequently, body weights during lactation were depressed while progeny mortality was increased. Dams given 50 or 100 mg/kg during late gestation and lactation were not adversely affected, but progeny body weights (predominantly males) were significantly decreased. Dams given up to 200 mg benzydamine/kg during the “critical period of organogenesis” displayed no signs of maternal toxicity, and no adverse reproductive or teratogenic effects were detected. Thus the demonstrated inhibition of reproductive performance by benzydamine appears to be due to an increase in maternal toxicity during late gestation and lactation. This difference, coupled with a greater adverse effect on male offspring during lactation, may suggest either a change in the pathway of benzydamine metabolism or a piperazine-like selectivity after repetative doses.     

Determination of taxifolin in Polygonum orientale and study on its antioxidant activity

A high-performance liquid chromatographic method was developed to determine taxifolin in Polygonum orientale. The chromatographic separation was performed on a Lichrospher ODS column. The mobile phase consisted of CH₃OH–0.3%CH₃COOH (35:65, v/v) with a flow-rate of 0.8 mL/min. The chromatographic peak area was linear to the concentration of taxifolin in the range of 5.0–100 μg/mL with a detection limit of 0.23 μg/mL. Good recovery results were also obtained with the range of 97.4–101.2%. In addition, radical scavenging capacity of taxifolin was studied by means of 1,1-diphenylpicrylhydrazyl free radical. The IC₅₀ value was 4.11 μmol/L, which indicated that taxifolin had a very potent antioxidant activity.     

Synthesis of novel triheterocyclic thiazoles as anti-inflammatory and analgesic agents

Triheterocyclic thiazoles containing coumarin and carbostyril (1-aza coumarin) have been synthesized by the reaction of the in situ generated 4-thioureidomethyl carbostyril and 3-bromoacetyl coumarins. The new compounds have been tested for their in vivo analgesic and anti-inflammatory activities. Qualitative SAR studies indicate that, the chloro substitution at C-7 in carbostyril and 6,8-dibromo substitution in the coumarin ring enhance anti-inflammatory activity. These compounds were also found to provide significant protection against acetic acid writhing in animal models. All the compounds have been characterized by IR, (1)H NMR, (13)C NMR and mass spectrometry.     

茶树菇水煎液对环磷酰胺诱发小鼠基因突变的保护作用

目的 研究茶树菇水煎液对环磷酰胺诱发KM小鼠突变的保护作用.方法 用改进的小鼠骨髓细胞微核实验和小鼠精子畸形实验方法,研究茶树菇水煎液对环磷酰胺诱发小鼠骨髓细胞微核和精子畸形的保护作用.结果 给40 mg/kg环磷酰胺后,茶树菇水煎液低、中、高剂量组及阳性对照组的微核率、精子畸形率均比阴性对照组显著升高(P＜0.01),茶树菇水煎液高剂量组的微核率18.2‰,显著低于阳性对照组微核率25.8‰(P＜0.01),茶树菇水煎液高剂量组的精子畸形率58.7‰,显著低于阳性对照组精子畸形率76.5‰(P ＜0.01).结论 茶树菇水煎液对环磷酰胺诱发小鼠骨髓细胞微核和精子畸形有保护作用.