25-hydroxyvitamin D, 24, 25-dihydroxyvitamin D and 1,25-dihydroxyvitamin D in human cerebrospinal fluid

Summary Samples of CSF and plasma were obtained simultaneously from 46 adult patients who had no endocrine disorders and were undergoing routine diagnostic lumbar puncture because of suspected or proved prolapse of a disc. Concentrations of 25-OHD, 24,25(OH)₂D and 1,25(OH)₂D were measured. The samples were purified by column chromatography and fractionated by HPLC. In the appropriate fractions the vitamin D metabolites were measured by PBA, and cytoreceptor assay. The results were as follows (median, range in brackets): 25-OHD in CSF 8.3 ng/ml (2.0–24.8), in plasma 14.5 ng/ml (7.0–36.0). 24,25(OH)₂D in CSF 1.8 ng/ml (0.3–4.6) and 2.5 ng/ml (0.4–4.7) in plasma. 1.25(OH)₂ D in CSF 25.0 pg/ml (2.2–39.0) and 31.0 pg/ml (10.1–55.0) in plasma. The correlations between plasma and CSF concentrations were as follows: 25-OHDr=0.479 (P<0.001); 24,25(OH)₂Dr=0.815 (P<0.001) and for 1.25(OH)₂Dr=0.497 (P<0.001).Our findings showed vitamin D metabolites to be present in human CSF.Abbreviations Ca Calcium - CSF Cerebrospinal fluid - Vitamin D₃ Cholecalciferol - CPM Counts per min - 24, 25 (OH)₂D 24, 25-dihydroxyvitamin D₃ - 1,25(OH)₂D 1,25-dihydroxyvitamin D₃ - Vitamin D₂ Ergocalciferol - HPLC High-pressure liquid chromatography - 25OHD 25-hydroxyvitamin D₃ - PTH Parathyroid hormone - PBA Protein binding assay - RIA Radioimmunoassay - D-CaBP Vitamin D dependent calcium-binding protein     

骨折患者血清25-羟基维生素D和甲状旁腺素水平与骨密度的相关性

目的探究骨折患者血清25-羟基维生素D(250HD)和甲状旁腺素(PTH)水平与骨密度的相关性。方法选择2015年12月至2017年12月于我院骨科住院治疗的110例骨折患者作为研究对象,所有患者均行骨密度检查,根据骨密度T值将患者分为正常组、骨质减少组、骨质疏松组。测定骨质疏松四项标志物即血清250HD、PTH、血清1型胶原氨基端前肽(PINP)、β胶原降解产物(β-CTX)水平。结果正常组各部位骨密度值显著高于骨量减少组、骨质疏松组(P<0.05);骨量减少组各部位骨密度值显著高于骨质疏松组,差异具有统计学意义(P <0.05);骨量正常患者血清250HD显著高于骨量减少、骨质疏松组(P <0. 05);血清PINP、PTH水平均显著低于骨量减少、骨质疏松组(P <0. 05);相关性分析示骨质疏松患者血清250HD与腰椎1-4、全髓关节及股骨颈骨密度值呈正相关关系(P<0. 05)。结论骨质疏松患者血清中250HD明显下降,与患者骨密度呈正相关,PTH水平虽在骨质疏松患者中明显上升,但其与骨密度无相关性。     

Hypercalcemia and elevated serum 1,25-dihydroxyvitamin D3 in a patient with hodgkin's lymphoma

Summary A 74-year-old woman was hospitalized because of decreased appetite, fatigue, and weight loss. The laboratory examination revealed hypercalcemia, a slightly increased serum creatinine level, and a markedly elevated serum level of 1,25-dihydroxyvitamin D₃. The most important finding the physical examination revealed was enlarged inguinal lymph nodes. A biopsy disclosed lymphocyte-depleted Hodgkin's disease. After steroids, but not after calcitonin, both the elevated calcitriol concentration and serum calcium normalized. In spite of intensive chemotherapy, a further episode with hypercalcemia occurred and increased 1,25-dihydroxyvitamin D₃ serum levels were observed. According to the available evidence it seems probable that the humoral hypercalcemia in this patient resulted from production of 1,25-dihydroxyvitamin D₃ in the tumor.Abbreviations 1,25(OH)₂D₃ 1,25-dihydroxyvitamin D₃ - iPTH serum immunoreactive parathyroid hormone     

Elevated 1,25-dihydroxyvitamin D and normocalcaemia in presumed familial Williams syndrome

Two brothers with Williams syndrome without hypercalcaemia are presented. One boy died during the first month of life. His brother also had the typical phenotypic features of the elfin facies. He developed severe microcephaly and cataract and died at the age of 9 years. The skeleton was osteosclerotic at birth, and became generally osteoporotic at the age of 2 years. He had persistently elevated 1,25-dihydroxyvitamin D levels during the first 2 years of life, in spite of normocalcaemia. At autopsy, microcalcifications were found in the brain and kidneys. The present report underscores the familial occurrence of Williams syndrome of severe degree. Elevated 1,25-dihydroxyvitamin D levels without hypercalcaemia have not been reported previously, and may suggest causal heterogeneity of the Williams syndrome.     

湛江市区学龄前儿童25-羟维生素D水平研究

目的 调查湛江地区学龄前（0~6岁）健康儿童25-羟维生素D[25（OH）D]水平,了解该人群中维生素D的健康状态。方法选取2017年6月1日~2018年5月31日在我检验中心进行体检的0~6岁儿童共3164例,测定25（OH）D水平,比较不同性别、不同季节的25（OH）D水平。结果 湛江市儿童血清25（OH）D总体水平为（42.75±12.20）ng/ml,男童为（42.27±11.88）ng/ml、女童为（42.93±12.58）ng/ml,不同性别儿童的25（OH）D水平差异无统计学意义（P＞0.05）。25（OH）D缺乏和不足的比例随着儿童年龄的增加而增多;秋季25（OH）D水平较其他季节略低。结论 湛江市区0~6岁儿童中缺乏和不足率均随年龄的成长而增高,应积极采取有效措施监督和提高儿童维生素D水平,以减少维生素D相关疾病的发生,提高健康水平。     

孕妇血清维生素A、维生素E、25-羟基维生素D与 子痫前期的相关性研究

目的 探讨孕妇血清维生素A、维生素E、25-羟基维生素D水平与子痫前期的相关性。方法 选择2016年3月~2017年10月在我院产检的单胎孕妇为研究对象,其中子痫前期组72例,健康对照组30例。分析血清维生素A、维生素E、25-羟基维生素D水平与子痫前期的相关性及其诊断价值。结果 在孕10~14周时,两组孕妇孕血清维生素A、维生素E 、25-羟基维生素D水平比较,差异无统计学意义（P＞0.05）;孕24~28周时,子痫前期组血清维生素A、维生素E 、25-羟基维生素D水平低于对照组水平（P＜0.05）;多因素Logistic回归分析结果显示,孕24~28周血清维生素A、维生素E、25-羟基维生素D水平与子痫前期发病具有显著的相关性;回归模型显示,血清维生素A诊断子痫前期的AUC为0.976,95%CI为0.931~1.000,当界值为0.46时,预测子痫前期发病的灵敏度为95.70%,特异度为72.50%;维生素E诊断子痫前期的AUC为0.820,95%CI为0.725~0.914,当界值为0.28时,预测子痫前期发病的灵敏度为76.70%,特异度为77.80%;25-羟基维生素D诊断子痫前期的AUC为0.789,95%CI为0.681~0.898,当界值为0.33时,预测子痫前期发病的灵敏度为73.30%,特异度为81.90%;血清维生素A+维生素E+25-羟基维生素D诊断子痫前期的AUC为0.988,95%CI为0.966~1.000。当界值为0.55时,预测子痫前期发病的灵敏度为96.70%,特异度为95.80%。结论 在孕10~14周时,血清维生素A、维生素E、25-羟基维生素D水平下降与子痫前期发病无关;在孕24~28周时,血清维生素A、维生素E、25-羟基维生素D水平下降与子痫前期发病显著相关,孕晚期血清维生素A、维生素E、25-羟基维生素D可作为子痫前期的关键指标。     

25-hydroxyvitamin D concentration, vitamin D intake and joint symptoms in postmenopausal women

Introduction

Low 25-hydroxyvitamin D (25(OH) D) concentrations have been associated with radiologic worsening of osteoarthritis in some reports. However, the results are mixed and few studies have evaluated associations between 25(OH) D concentrations and both total vitamin D intake and clinical joint symptoms.

Study design

Cross-sectional analyses of information from a subset of 1993 postmenopausal women obtained at baseline entry in the Women's Health Initiative Calcium plus Vitamin D clinical trial.

Main Outcome Measures

25(OH) D concentration, total vitamin D intake (diet plus supplements), presence and severity of joint pain and joint swelling.

Results

The 25(OH) D levels were commonly low with 53% having deficient (<50 nmol/L) and only 17% having sufficient (>72 nmol/L) levels. Joint pain (reported by 74%) and joint swelling (reported by 34%) were also commonly reported. 25(OH) D concentrations were modestly correlated with total vitamin D intake (R = 0.29, p < 0.0001); however, considerable variability in 25(OH) D concentrations for a given vitamin D intake was seen. In adjusted linear regression models, lower serum 25(OH) D concentrations were associated with higher average joint pain score (P = 0.01 for trend) with differences most apparent in the lowest 25(OH) D levels sextile.

Conclusions

Relatively low 25(OH) D levels and a high frequency of joint symptoms were common in this population of postmenopausal women. Total vitamin D intake was only modestly associated with 25(OH) D. Low serum 25(OH) D concentrations were associated with higher joint pain scores. These findings can inform the design of future intervention trials.     

性别、年龄和季节对血清25-羟维生素D水平的影响

目的探讨性别、年龄和季节对血清25-羟维生素D(25OHD)的影响。方法调取2014年12月至2018年10月北京协和医院血清25OHD检测结果,共计34062例。采用罗氏e601自动分析仪及其配套试剂检测血清25OHD。应用回归分析评估性别、年龄和季节对血清25OHD水平的影响。结果男性血清25OHD水平明显高于女性(P<0.001)。血清25OHD在8月份达到高峰为22.1 ng/mL。夏季对25OHD水平影响最大(标准化系数=0.273)。结论性别和季节显著影响血清25OHD水平。     

The association between serum 25-hydroxyvitamin D concentrations and serum lipids in the Southern Thai population

IntroductionDyslipidaemia is a major risk factor for cardiovascular diseases (CVD). Vitamin D deficiency has been found to be associated with CVD. However, the relationships between vitamin D and lipids are inconsistent. The aim of this study was to investigate the relationship between vitamin D status and serum lipids in Southern Thai subjects.Material and methodsA total of 726 healthy subjects in Southern Thailand were enrolled in the study. Serum 25-hydroxyvitamin D (25(OH)D), lipid profiles, fasting plasma glucose, anthropometric data, blood pressure, and body composition were measured. The relationship between serum 25(OH)D levels and biochemical data was evaluated by partial correlation and multiple linear regression analyses. The association of serum 25(OH)D levels with dyslipidaemia was analysed using multivariate regression analysis.ResultsSerum 25(OH)D levels were negatively correlated with body mass index (BMI), total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), and body composition parameters after adjusting for age in women. Multiple linear regression analysis showed that TC and BMI were independent predictors for 25(OH)D concentrations in women. Multivariate logistic regression analysis showed that the odds ratio of hypertriglyceridaemia (OR = 0.51; 95% CI: 0.32–0.80, p = 0.004) and reduced high-density lipoprotein cholesterol (HDL-C) (OR = 0.43; 95% CI: 0.26–0.71, p = 0.001) were significantly lower in vitamin D sufficiency when compared with hypovitaminosis D in women.ConclusionsVitamin D sufficiency could reduce risk of hypertriglyceridaemia and reduced HDL-C, particularly in women, suggesting that vitamin D sufficiency may have beneficial effects on lipids and a decreased risk for CVD in Thai women.     

妊娠早期血清25(OH)D3水平与妊娠期糖尿病前期的关系研究

目的探讨妊娠期糖尿病前期与血清25(OH)D3水平的关系,为妊娠期糖尿病防治及其危险因素的研究提供新的思路。方法选择2017年1月至2018年1月在南通大学附属海安医院产科初诊并进行产前检查的妇女800例,入选妊娠期糖尿病前期348例,选取正常孕妇150例作为对照组。对2组孕妇的BMI、空腹血糖、服糖后1h血糖、服糖后2h血糖、空腹胰岛素及25(OH)D3等指标进行统计分析与比较。结果妊娠期糖尿病前期组在BMI、空腹血糖、服糖后2h血糖均高于对照组(P<0.01);妊娠期糖尿病前期组25(OH)D3明显低于对照组(P<0.01);两组在年龄、空腹胰岛素、服糖1h血糖上的差异无统计学意义(P>0.05)。血清25(OH)D3水平与服糖1h血糖及服糖2h血糖呈负相关(P<0.05),而与年龄、BMI、空腹血糖及空腹胰岛素无显著相关性(P>0.05)。25(OH)D3水平与妊娠期糖尿病前期呈负相关。结论维生素D水平可作为妊娠期糖尿病的预测指标,对预防妊娠期糖尿病的发生、发展具有重要意义。     

Serum 25-hydroxyvitamin D and IgE – a significant but nonlinear relationship

Background:  Hormonal vitamin D system affects the determination of T-cell responses. It is unknown if there is an association between vitamin D status and allergic conditions. Our aim was to investigate differences in serum IgE concentrations by vitamin D status [measured by 25(OH)D] and by a genetic variation in a key vitamin D activation enzyme (CYP27B1) previously shown to be associated with type 1 diabetes.
Methods:  9377 participants in the 1958 British birth cohort completed a biomedical assessment at 45 years of age ; 7288 eligible participants had data on 25(OH)D and IgE, with 6429 having further information on CYP27B1 genotype (−1260C>A).
Results:  There was a nonlinear association between 25(OH)D and IgE ( P -value for curvature = 0.0001). Compared with the reference group with the lowest IgE concentrations [25(OH)D 100–125 nmol/l], IgE concentrations were 29% higher (95% CI 9–48%) for participants with the 25(OH)D <25 nmol/l, and 56% higher (95% CI 17–95%) for participants with 25(OH)D >135 nmol/l (adjusted for sex, month, smoking, alcohol consumption, time spent outside, geographical location, social class, PC/TV time, physical activity, body mass index and waist circumference). CYP27B1 genotype was associated with both 25(OH)D (difference for A vs. C allele: 1.88%, 95% CI 0.37–3.4%, P  = 0.01) and IgE concentrations (−6.59%, −11.6% to −1.42%, P  = 0.01).
Conclusions:  These data suggest that there may be a threshold effect with both low and high 25(OH)D levels associated with elevated IgE concentrations. The same CYP27B1 allele that is protective of diabetes was associated with increased IgE concentrations.     

Vitamin D and aging: beyond calcium and bone metabolism

Background

Low serum 25-hydroxyvitamin D (25[OH]D) levels are common and may be associated with morbidity and mortality (and indeed with frailty more generally). This association is not restricted to the links between vitamin D and calcium and bone metabolism.

Objective

To review the influences of vitamin D on the aging process other than those related to bone and calcium. Its effect on mortality is also assessed.

Methods

The PubMed database was searched for English-language articles relating to vitamin D, using the following MeSH terms: vitamin D, mortality, cardiovascular diseases, and frailty. In addition, searches were carried out with Google.

Results

Although some of the reported results have proved controversial, overall the evidence seems to support an association between low serum 25[OH]D levels and mortality rates (all-cause and cardiovascular). Frailty is a condition frequently associated with low serum 25[OH]D levels.

Conclusion

The aging process and mortality are associated with low vitamin D levels. Prospective controlled trials are warranted to determine whether vitamin D supplements can increase longevity and reduce the incidence of certain conditions.     

外周维生素D结合蛋白、25-羟维生素D和原发性肝细胞癌风险的关系

目的 25-羟维生素D[25(OH) D)]已被证明与原发性肝细胞癌(HCC)的发生有关,但对于维生素D结合蛋白(VDBP)在原发性肝细胞癌发生中的作用却知之甚少.作者检测了25(OH)D的主要载体维生素D结合蛋白(VDBP),研究其在25(OH)D与原发性肝细胞癌风险中的作用.方法 收集146例HCC患者和249名对照组的血样,检测血浆VDBP和25(OH)D的浓度.采用logistic回归计算比值比(OR)和95％可信区间(CI),评估外周血VDBP、25(OH)D与原发性肝细胞癌的发生风险之间的关系.结果 血浆VDBP浓度与HCC风险之间呈负相关;血浆25(OH)D与HCC风险呈正相关.只有在25(OH)D浓度高于中位数的人群中,VDBP升高显著,降低了HCC风险.在VDBP浓度低于中位数的人群中,高浓度的25(OH)D显著提高了HCC风险.结论 高浓度的VDBP可结合更多的25(OH)D,减少游离25(OH)D的生物利用度,同时检测VDBP和25(OH)D水平对于确定维生素D与HCC风险的相关性非常重要.     

人体25羟维生素D的竞争蛋白结合测定及冬夏季正常值

本文采用二氯甲烷/乙醇粗提血浆,高压液相色谱精分,竞争蛋白结合测定法求得25羟维生素D值,夏季北京居民正常值为13.2±5.9ng/ml(33.0±14.8nmol/L)(n=68),其中男性为13.0±5.7ng/ml(32.5±14.3nmol/L)(n=39),女性为13.5±6.2ng/ml(33.8±15.5nmol/L)(n=29);冬季正常值为10.4±3.8ng/ml(26.1±9.6nmol/L)(n=45),其中男性为10.2±4.2ng/ml(25.4±10.4nmol/L)(n=22),女性为10.7±3.6ng/ml(26.6±8.9nmol/L)(n=23),性别间无显著性差异,夏冬季P值均大于0.5;夏季值之间差异显著(P<0.01)。 文中还对响影测定的有关问题作了讨论。     

Relationship between vitamin D, parathyroid hormone, and bone mineral density in elderly Koreans

There is controversy regarding definition of vitamin D inadequacy. We analyzed threshold 25-hydroxyvitamin D (25[OH]D) below which intact parathyroid hormone (iPTH) increases, and examined age- and sex-specific changes of 25(OH)D and iPTH, and association of 25(OH)D and iPTH with bone mineral density (BMD) in elderly Koreans. Anthropometric parameters, serum 25(OH)D and iPTH, lumbar spine and femur BMD by dual-energy radiography absorptiometry (DXA) were measured in 441 men and 598 postmenopausal women. iPTH increased below serum 25(OH) of 36.7 ng/mL in men, but failed to reach plateau in women. Femur neck BMD above and below threshold differed when threshold 25(OH)D concentrations were set at 15-27.5 ng/mL in men, and 12.5-20 ng/mL in postmenopausal women. Vitamin D-inadequate individuals older than 75 yr had higher iPTH than those aged ≤ 65 yr. In winter, age-associated iPTH increase in women was steeper than in summer. In conclusion, vitamin D inadequacy threshold cannot be estimated based on iPTH alone, and but other factors concerning bone health should also be considered. Older people seemingly need higher 25(OH)D levels to offset age-associated hyperparathyroidism. Elderly vitamin D-inadequate women in the winter are most vulnerable to age-associated hyperparathyroidism.     

维生素缺乏对老年人髋部骨折的影响

目的 对比老年髋部骨折患者与老年非骨折的骨病患者维生素水平及缺乏情况,分析维生素缺乏对老年髋部骨折的影响。方法 回顾性病例对照研究。纳入2020年11月—2022年2月深圳市第二人民医院均行血清维生素水平检测的老年髋部骨折患者172例（骨折组）及老年非骨折的髋膝关节骨病患者345（非骨折组）例。骨折组患者中男50例、女122例,年龄65~97（81.0±8.2）岁;非骨折组中男89例、女256例,年龄65~90（72.1±5.9）岁。采用液相色谱串联质谱法检测血清维生素A、B1、B2、B3、B5、B6、B9、E、K1,以及25-羟基维生素D（D2和D3）的含量,将维生素水平低于正常值下限定义为相应维生素缺乏。观察指标：（1）观察骨折组与非骨折组是否缺乏维生素,并比较2组患者维生素水平的差异。（2）将标准化后的维生素水平作为自变量,骨折与否作为因变量,年龄和性别作为协变量,采用多因素logistic回归分析标准化后维生素水平对老年髋部骨折的影响。结果 （1）骨折组患者维生素A、B1、B3、B9、E缺乏情况更为严重,骨折组占比分别为62.8% （59/94）、62.4% （58/93）、19.4% （18/93）、50.0% （52/104）、14.9% （14/94）,非骨折组为20.2% （49/243）、41.2% （100/243）、5.0% （12/241）、16.3% （41/251）、4.5% （11/243）,差异均有统计学意义（χ²=56.49、12.15、15.82、43.10、10.61,P值均<0.05）。骨折组患者的血清25-羟维生素D、D3,以及维生素A、B1、B3、B5、B6、B9、E、K1水平较非骨折组明显降低,差异均有统计学意义（Z=-4.41、-2.53、-7.08、-3.43、-5.25、-2.08、-2.46、-6.80、-3.26、-7.93,P值均<0.05）;25-羟维生素D2和维生素B2水平2组差异均无统计学意义（P值均>0.05）。（2）多因素回归分析显示,维生素A[比值比（OR）=0.30,95%可信区间（CI）0.20~0.45]、维生素K1（OR=0.31,95% CI 0.21~0.46）、维生素B9（OR=0.33,95% CI 0.23~0.47）、维生素B3（OR=0.50,95% CI 0.36~0.70）、维生素B5（OR=0.50,95% CI 0.37~0.69）、维生素B1（OR=0.52,95% CI 0.38~0.72）、维生素E（OR=0.61,95% CI 0.45~0.83）、25-羟基维生素D（OR=0.70,95% CI 0.55~0.89）和维生素B6（OR=0.71,95% CI 0.54~0.95）水平的降低均会导致老年髋部骨折的风险增加（P值均<0.05）。结论 老年髋部骨折患者和老年非骨折的髋膝关节退行性骨病患者都普遍存在各种维生素缺乏,而且以25-羟基维生素D的缺乏最为严重。髋部骨折患者相对于非骨折患者的多种维生素水平更低,血清维生素A、B1、B3、B5、B6、B9、E、K1、25-羟基维生素D水平的降低会增加髋部骨折的风险。     

1Alpha,25-dihydroxyvitamin D3 treatment does not alter neuronal cyclooxygenase-2 expression in the cerebral cortex after stroke

The inducible prostaglandin synthase, cyclooxygenase-2, is upregulated in response to cerebral ischemia and contributes to potentiation of oxidative injury. Cyclooxygenase-2 expression is regulated by retinoic acid receptors, which form heterodimers with vitamin D receptors and vitamin D. In addition, vitamin D has been reported to have neuroprotective qualities. The aim of this study was to examine whether the biologically active vitamin D₃-metabolite 1α,25-dihydroxyvitamin D₃ (1,25-D₃), influences the expression of inducible cyclooxygenase-2 in photothrombotically lesioned brain or is part of an independent neuroprotective mechanism. We compared groups of nonlesioned control rats and infarcted animals, which were treated with either 1,25-D₃ or solvent at different times postlesion. In control animals, cyclooxygenase-2 immunoreactivity was readily evident in almost all cortical neurons of layers II/III as well as in a few pyramidal cells in layer V. Following photothrombotic infarction of the right cortical hindlimb area, there was a significant, but transient, increase in cyclooxygenase-2 labeling which was restricted to neurons of the injured hemisphere in both 1,25-D₃-treated and solvent-treated rats. Highest levels of cyclooxygenase-2 immunoreactivity were seen at 12 and 24 h postlesion, followed by a gradual decrease at later time points. However, no significant differences were detected between 1,25-D₃-treated and solvent-treated lesioned rats, indicating that postischemic neuronal cyclooxygenase-2 upregulation is not influenced by 1,25-D₃. It is concluded that the neuroprotective effect of 1,25-D₃ does not depend on modulations of neuronal COX-2 expression caused by postlesional hyperexcitation.     

HBeAg阴性慢性乙型肝炎患者血清25-羟基维生素D3的检测及临床意义

目的 探究HBeAg阴性慢性乙型肝炎患者血清25-羟基维生素D3的水平变化及临床意义。方法 选取2015年3月~2018年5月81例HBeAg阴性且非肝硬化的慢性乙型肝炎患者作为观察组,依据血清谷丙转氨酶（ALT）水平分为观察组A 41例（ALT处于正常水平且HBV DNA水平＜2000 IU/ml,持续时间高于6个月）和观察组B 40例（ALT处于升高水平且HBV DNA水平≥2000 IU/ml,持续时间高于6个月）。同时选取同期健康体检人群40例作为对照组。分别检测三组血清25-羟基维生素D3的浓度。结果 观察组血清25-羟基维生素D3低于健康人群,统计学意义显著（P＜0.001）,三组间比较,统计学意义显著（P＜0.001）。观察组患者血清25-羟基维生素D3与年龄、BMI、ALT、AST、Hb、WBC、PLT及AFP均无明显相关性（P＞0.05）。而与HBV DNA含量间存在显著负相关（r=-3.981,P＜0.05）。结论 HBeAg阴性慢性乙型肝炎患者血清25-羟基维生素D3存在异常,并与乙肝病毒复制相关,可能参与机体和乙肝病毒的免疫反应过程。