Caffeine Attenuates Acute Growth Hormone Response to a Single Bout of Resistance Exercise

The purpose of this study was to investigate the effects of caffeine consume on substrate metabolism and acute hormonal responses to a single bout of resistance exercise (RE). Ten resistance-trained men participated in this study. All subjects performed one repetition maximum (1RM) test and then performed two protocols: caffeine (CAF, 6 mg·kg^-1) and control (CON) in counter balanced order. Subjects performed RE (8 exercises, 3 sets of 10 repetitions at 75% of 1RM) after caffeine or placebo ingestion one hour prior to RE. Blood samples collected prior to treatment ingestion (pre-60), immediately prior to RE (pre-exe), and 0, 15, 30 min post to RE (P0, P15, P30) for analysis of insulin, testosterone, cortisol, growth hormone, glucose, free fatty acid and lactic acid. Each experiment was separated by seven days. In this study, statistical analysis of a two-way analysis of variance (treatment by time) with repeated measures was applied. After ingesting caffeine, the concentrations of free fatty acid (pre- exe, P0, P15, P30) in CAF were significantly higher than CON (p < 0.05). Additionally, the responses of GH (P0, P15, P30) in CAF were significantly lower than CON (p < 0.05), whereas the concentrations of insulin, testosterone and cortisol were not different between CAF and CON (p < 0.05) after RE. The results of this study indicated that caffeine ingestion prior to RE might attenuate the response of GH. This effect might be caused by the elevation in blood FFA concentration at the beginning of RE.

Key points

• Caffeine ingestion may attenuate the response of GH to a single bout of resistance exercise.
• The depression of GH response may be caused by the elevation in serum FFA concentration at the beginning of resistance exercise.
• Caffeine ingestion before resistance exercise may not alert the concentration of cortisol and testosterone.

Key words: Nutritional supplementation, growth hormone, free fatty acid, ergogenic aids     

Salivary Cortisol Responses and Perceived Exertion during High Intensity and Low Intensity Bouts of Resistance Exercise

The purpose of this study was to measure the salivary cortisol response to different intensities of resistance exercise. In addition, we wanted to determine the reliability of the session rating of perceived exertion (RPE) scale to monitor resistance exercise intensity. Subjects (8 men, 9 women) completed 2 trials of acute resistance training bouts in a counterbalanced design. The high intensity resistance exercise protocol consisted of six, ten-repetition sets using 75% of one repetition maximum (RM) on a Smith machine squat and bench press exercise (12 sets total). The low intensity resistance exercise protocol consisted of three, ten-repetition sets at 30% of 1RM of the same exercises as the high intensity protocol. Both exercise bouts were performed with 2 minutes of rest between each exercise and sessions were repeated to test reliability of the measures. The order of the exercise bouts was randomized with least 72 hours between each session. Saliva samples were obtained immediately before, immediately after and 30 mins following each resistance exercise bout. RPE measures were obtained using Borg’s CR-10 scale following each set. Also, the session RPE for the entire exercise session was obtained 30 minutes following completion of the session. There was a significant 97% increase in the level of salivary cortisol immediately following the high intensity exercise session (P<0.05). There was also a significant difference in salivary cortisol of 145% between the low intensity and high intensity exercise session immediately post-exercise (P<0.05). The low intensity exercise did not result in any significant changes in cortisol levels. There was also a significant difference between the session RPE values for the different intensity levels (high intensity 7.1 vs. low intensity 1.9) (P<0.05). The intraclass correlation coefficient for the session RPE measure was 0.95. It was concluded that the session RPE method is a valid and reliable method of quantifying resistance exercise and that salivary cortisol responds promptly to the exercise load.

Key Points

• The present study showed that salivary cortisol responses were significantly different immediately post exercise between the low intensity and high intensity exercise sessions
• Salivary measures of cortisol can be used to delineate between high and low intensity resistance exercise bouts.
• The session RPE method appears to be a reliable method of quantifying resistance exercise

Key words: Weight lifting, stress, endocrine effects     

Acute Bone Marker Responses to Whole-Body Vibration and Resistance Exercise in Young Women

Whole-body vibration (WBV) augments the musculoskeletal effects of resistance exercise (RE). However, its acute effects on bone turnover markers (BTM) have not been determined. This study examined BTM responses to acute high-intensity RE and high-intensity RE with WBV (WBV + RE) in young women (n = 10) taking oral contraceptives in a randomized, crossover repeated measures design. WBV + RE exposed subjects to 5 one-minute bouts of vibration (20 Hz, 3.38 peak-peak displacement, separated by 1 min of rest) before RE. Fasting blood samples were obtained before (Pre), immediately after WBV (PostVib), immediately after RE (IP), and 30-min after RE (P30). Bone alkaline phosphatase did not change at any time point. Tartrate-resistant acid phosphatase 5b significantly increased (p < 0.05) from the Pre to PostVib, then decreased from IP to P30 for both conditions. C-terminal telopeptide of type I collagen (CTX) significantly decreased (p < 0.05) from Pre to PostVib and from Pre to P30 only for WBV + RE. WBV + RE showed a greater decrease in CTX than RE (?12.6% ± 4.7% vs ?1.13% ± 3.5%). In conclusion, WBV was associated with acute decreases in CTX levels not elicited with RE alone in young women.     

Metabolic and Cardiovascular Responses to Upright Cycle Exercise with Leg Blood Flow Reduction

The purpose of this study was to examine the metabolic and cardiovascular response to exercise without (CON) or with (BFR) restricted blood flow to the muscles. Ten young men performed upright cycle exercise at 20, 40, and 60% of maximal oxygen uptake, VO₂max in both conditions while metabolic and cardiovascular parameters were determined. Pre-exercise VO₂ was not different between CON and BFR. Cardiac output (Q) was similar between the two conditions as a 25% reduction in stroke volume (SV) observed in BFR was associated with a 23% higher heart rate (HR) in BFR compared to CON. As a result rate-pressure product (RPP) was higher in the BFR but there was no difference in mean arterial pressure (MAP) or total peripheral resistance (TPR). During exercise, VO₂ tended to increase with BFR (~10%) at each workload. Q increased in proportion to exercise intensity and there were no differences between conditions. The increase in SV with exercise was impaired during BFR; being ~20% lower in BFR at each workload. Both HR and RPP were significantly greater at each workload with BFR. MAP and TPR were greater with BFR at 40 and 60% VO₂max. In conclusion, the BFR employed impairs exercise SV but central cardiovascular function is maintained by an increased HR. BFR appears to result in a greater energy demand during continuous exercise between 20 and 60% of control VO₂max; probably indicated by a higher energy supply and RPP. When incorporating BFR, HR and RPP may not be valid or reliable indicators of exercise intensity.

Key points

• Blood flow reduction (BFR) employed impairs stroke volume (SV) during exercise, but central cardiovascular function is maintained by an increased heart rate (HR).
• BFR appears to result in a greater energy demand during continuous exercise between 20 and 60% of control VO₂max;
• Probably indicated by a higher energy supply (VO₂) and rate-pressure product (HR x systolic blood pressure).

Key words: Aerobic exercise, doppler echocardiography, apparent exercise intensity, occlusion     

Acute Pro- and Anti-Inflammatory Responses to Resistance Exercise in Patients with Coronary Artery Disease: A Pilot Study

Little is known about the inflammatory effects of resistance exercise in healthy and even less in diseased individuals such as cardiac patients. The purpose of this study was to examine the acute pro- and anti-inflammatory responses during resistance exercise (RE) in patients with coronary artery disease. Eight low risk patients completed two acute RE protocols at low (50% of 1 RM; 2x18 rps) and moderate intensity (75% of 1 RM; 3x8 rps) in random order. Both protocols included six exercises and had the same total load volume. Blood samples were obtained before, immediately after and 60 minutes after each protocol for the determination of lactate, TNFα, INF-γ, IL-6, IL-10, TGF-β₁, and hsCRP concentrations. IL-6 and IL-10 levels increased (p < 0.05) immediately after both RE protocols with no differences between protocols. INF-γ was significantly lower (p < 0.05) 60 min after the low intensity protocol, whereas TGF-β1 increased (p < 0.05) immediately after the low intensity protocol. There were no differences in TNF-& and hs-CRP after both RE protocols or between protocols. The above data indicate that acute resistance exercise performed at low to moderate intensity in low risk, trained CAD patients is safe and does not exacerbate the inflammation associated with their disease.

Key points

• Acute resistance exercise is safe without exacerbating inflammation in patients with CAD.
• Both exercise intensities (50 and 75% of 1 RM) elicit desirable pro-and anti-inflammatory responses.
• With both exercise intensities (50 and 75% of 1 RM) acceptable clinical hemodynamic alterations were observed.

Key words: Resistance exercise, inflammation, cardiac rehabilitation     

Cardiorespiratory and Metabolic Responses to Loaded Half Squat Exercise Executed at an Intensity Corresponding to the Lactate Threshold

This study was designed to identify the blood lactate threshold (LT2) for the half squat (HS) and to examine cardiorespiratory and metabolic variables during a HS test performed at a work intensity corresponding to the LT2. Twenty-four healthy men completed 3 test sessions. In the first, their one-repetition maximum (1RM) was determined for the HS. In the second session, a resistance HS incremental-load test was performed to determine LT2. Finally, in the third session, subjects performed a constant-load HS exercise at the load corresponding to the LT2 (21 sets of 15 repetitions with 1 min of rest between sets). In this last test, blood samples were collected for lactate determination before the test and 30 s after the end of set (S) 3, S6, S9, S12, S15, S18 and S21. During the test, heart rate (HR) was telemetrically monitored and oxygen consumption (VO₂), carbon dioxide production (VCO₂), minute ventilation (VE), respiratory exchange ratio (RER), ventilatory equivalent for O₂ (VE·VO₂^-1) and ventilatory equivalent for CO₂ (VE·VCO₂^-1) were monitored using a breath-by-breath respiratory gas analyzer. The mean LT2 for the participants was 24.8 ± 4.8% 1RM. Blood lactate concentrations showed no significant differences between sets 3 and 21 of exercise (p = 1.000). HR failed to vary between S6 and S21 (p > 1.000). The respiratory variables VO₂, VCO₂, and VE·VCO₂^-1 stabilized from S3 to the end of the constant-load HS test (p = 0.471, p = 0.136, p = 1.000), while VE and VE·VO₂^-1 stabilized from S6 to S21. RER did not vary significantly across exercise sets (p = 0.103). The LT2 was readily identified in the incremental HS test. Cardiorespiratory and metabolic variables remained stable during this resistance exercise conducted at an exercise intensity corresponding to the LT2. These responses need to be confirmed for other resistance exercises and adaptations in these responses after a training program also need to be addressed.

Key points

• It can be identified lactate threshold at half-squat.
• Exercise intensity is predominantly aerobic.
• The duration of the half-squat can be maintained over time, ~30 min of discontinuous exercise (21 sets, 15 repetitions, 1 min rest).
• Lactate threshold intensity may be suitable for older adults, sedentary individuals, patients or subjects with a lower functional capacity and even for resistance sports athletes.

Key words: Aerobic fitness/VO_2max, anaerobic threshold, exercise physiology, strength training     

Effect of Zoledronate on the Responses of Osteocytes to Acute Parathyroid Hormone

The bone anabolic effect of parathyroid hormone (PTH) therapy is blunted when used in patients who were previously on bisphosphonate treatment. Osteocytes may play a role in the bisphosphonate silencing effect on PTH therapy since bisphosphonates have been shown to reach the lacunocanalicular system. In vivo osteocyte studies pose a significant challenge. For the current study, we developed a simple method to isolate RNA from cortical bone enriched with osteocytes. Our purpose was to investigate how zoledronate (ZA) treatment modulates the responses of osteocytes and the bone marrow (BM) to acute PTH treatment. Mice received ZA treatment for 3 months and a single PTH injection prior to death. Bone was histomorphometrically evaluated. Gene expression was assessed at the RNA level in osteocytes and BM. Endothelial progenitor cells (EPCs) and γδT cells were analyzed in the BM and blood using flow cytometry. We found that ZA treatment altered bone responses to PTH. Expression of Sfrp4, a Wnt antagonist, was significantly increased in ZA-affected osteocytes. BM EPCs were increased in response to acute PTH but not when treatment was combined with ZA. ZA treatment augmented EPCs in the BM but not in blood, which suggests that ZA treatment may have differential effects between the BM and blood. These findings indicate that osteocytes and BM EPCs in mice on ZA treatment respond differently to acute PTH from those not receiving ZA. This may partially explain the mechanisms of previous reports that ZA therapy attenuates the anabolic effect of PTH in bone.     

Effects of a Single Bout of Resistance Exercise on Calcium and Bone Metabolism in Untrained Young Males

Although resistance exercise training appears to increase bone mineral density in the long term, a single bout of resistance exercise could paradoxically induce bone homeostasis disturbance, secondary to metabolic acidosis. To examine this, we obtained fasting blood and 24-hour urine samples from untrained male subjects for 5 subsequent days (control day, exercise day, and three post-exercise days), and investigated the effects of a single bout of resistance exercise on urinary calcium excretion and bone metabolism as indicated by sensitive biomarkers of bone formation and resorption. After an intense bout of resistance exercise, blood and urine became more acidic and renal net acid excretion significantly increased by 44% on the exercise day. Urinary calcium excretion significantly increased by 48% on the exercise day. Plasma procollagen type-I C-terminal concentration significantly decreased by 12% on the next day of the exercise and serum bone-specific alkaline phosphatase activity also significantly decreased by 13% and 9% on days 2 and 3, respectively, after the exercise. There was no significant change in serum osteocalcin concentration. Serum tartrate-resistant acid phosphatase activity significantly decreased by 15% on the day after the exercise and urinary deoxypyridinoline excretion decreased by 22% and 27% on days 1 and 3, respectively, after the exercise. These results suggest that the early response of bone to a bout of resistance exercise in untrained individuals was transient decreases in bone formation and resorption, whereas urinary calcium excretion increased. Received: 19 November 1996 / Accepted: 21 May 1997     

Effects of the Menstrual Cycle on Expiratory Resistance During Whole Body Exercise in Females

Our objective was to determine if the menstrual cycle affected expiratory resistance developed during progressive incremental exercise in females. Eleven females (age = 19.7 ± 1.1 yr., body mass = 58.9 ± 8.8 Kg, height = 1.65 ± 0.3 m) gave consent to participate in the study. Participants were studied during the follicular (day 7 ± 2 days following onset of menses) and luteal (day 21 ± 2 days following onset of menses) phases of their menstrual cycle. The expiratory resistance was significantly higher during the follicular phase at maximal workload versus the luteal phase (1.0 ± 0.06 cm H₂O/L/sec vs. 0.9 ± 0.07 cm H₂O/L/sec.: p¼ 0.05). No other differences were found in expiratory resistance, oxygen uptake or maximal heart rate during exercise. Results showed that the increase in expiratory resistance during the follicular phase of the menstrual cycle may be contributing to the changes in the pulmonary system of females as reported by other authors.

Key points

• During maximal exercise there was a significantly larger expiratory resistance during the follicular phase versus luteal phase of the female subjects menstrual cycle.
• Fluctuation in hormones (especially progesterone and/ or oestrogen) may contribute to changes in expiratory resistance.
• The increased expiratory resistance may be a contributing factor to the increased occurrence of expiratory flow limitation in female subjects.

Key words: airway resistance, female, menstrual cycle, exercise     

Acute Responses of Parathyroid Hormone and 1,25 Dihydroxyvitamin D3 to Unilateral Nephrectomy in Healthy Donors

Plasma immunoreactive parathyroid hormone (iPTH), 1,25(OH)₂D3calcium and phosphate and urinary creatinine, calcium and phosphatewere measured before and following unilateral nephrectomy insix kidney donors. Unexpectedly, plasma calcium rose, from 2.27±0.02mmol/l (mean±SEM) to 2.41±0.03 mmol/l on day 7and to 2.37±0.02 mmol/l on day 30 (P<0.02). A parallelrise in iPTH occurred, from 0.61±0.16 ng/ml initially,to 1.83±0.54 ng/ml on day 7 (P<0.05) and to 1.18±0.18on day 30 (P<0.01). The ratio of maximal tubular reabsorptionof phosphate to GFR (TmP/GFR) fell by day 2 (P<0.001), remainingreduced on day 30 (P<0.05). The significance of elevated iPTH in renal insufficiency wasfurther assessed by determining the time course of the disappearanceof iPTH after parathyroidectomy in three haemodialysis subjects.Fifty per cent baseline iPTH level occurred after an averageof 104.7 min, suggesting that the assay did not predominantlyrecognize C-terminal PTH fragments. By day 2, plasma 1,25(OH)₂D3had fallen from 34.3±4.5 pg/ml to 22.8±3.8 pg/ml(P<0.001), but by day 4 had regained its pre-nephrectomyvalue. Our results suggest that hypocalcaemia may not be thesole stimulus to parathyroid hormone secretion. It is speculatedthat reduction in circulating 1,25(OH)₂D3 may be involved.     

Spinal Cord and Cerebral Blood Flow Responses to Subarachnoid Injection of Local Anesthetics with and without Epinephrine

Subarachnoid anesthesia with lidocaine, mepivacaine, or tetracaine with and without added epinephrine (1:100 000) produced no demonstrable changes in average cerebral (CBF) or segmental spinal cord blood flow (SCBF) in 38 cats anesthetized with pentobarbital. Blood flow was measured by the injection of radioactive microspheres. Seven groups of cats received either lidocaine 15 mg, lidocaine 15 mg with epinephrine, mepivacaine 10 mg, mepivacaine 10 mg with epinephrine, tetracaine 5 mg, tetracaine 5 mg with epinephrine, or saline with epinephrine 1:100 000. Mean arterial pressure (MAP) decreased significantly (P less than 0.05) in Groups I-VI. Added epinephrine had no effect on the decrease in MAP. Amplitude of the somatosensory cortical evoked response decreased significantly in Groups I-VI, but did not change from control in Group VII. No significant change in CBF or SCBF was demonstrated in any group at any time. Plasma lidocaine and mepivacaine levels were significantly less at 5 min after subarachnoid injection in the groups receiving epinephrine compared to those not receiving epinephrine (P less than 0.05). The data appear to support the hypothesis of a vasoconstrictive reduction in systemic absorption of intrathecal local anesthetics, but suggest that significant segmental spinal cord ischemia does not occur. Maintenance of total flow in the face of a decrease in MAP suggests that autoregulation in brain and spinal cord may be maintained. Changes in regional SCBF or CBF may have been present but were not examined in this study. Further studies of brain and spinal cord blood flow dynamics, regional flow changes, and regulation of flow after intrathecal agents are necessary.     

Effects of Low-Intensity Cycle Training with Restricted Leg Blood Flow on Thigh Muscle Volume and VO2MAX in Young Men

Concurrent improvements in aerobic capacity and muscle hypertrophy in response to a single mode of training have not been reported. We examined the effects of low-intensity cycle exercise training with and without blood flow restriction (BFR) on muscle size and maximum oxygen uptake (VO₂max). A group of 19 young men (mean age ± SD: 23.0 ± 1.7 years) were allocated randomly into either a BFR-training group (n=9, BFR-training) or a non-BFR control training group (n=10, CON-training), both of which trained 3 days/wk for 8 wk. Training intensity and duration were 40% of VO₂max and 15 min for the BFR-training group and 40% of VO₂max and 45 min for the CON-training group. MRI-measured thigh and quadriceps muscle cross-sectional area and muscle volume increased by 3.4-5.1% (P < 0.01) and isometric knee extension strength tended to increase by 7.7% (p < 0.10) in the BFR-training group. There was no change in muscle size (~0.6%) and strength (~1.4%) in the CON-training group. Significant improvements in VO₂max (6.4%) and exercise time until exhaustion (15.4%) were observed in the BFR-training group (p < 0.05) but not in the CON-training group (-0.1 and 3. 9%, respectively). The results suggest that low-intensity, short-duration cycling exercise combined with BFR improves both muscle hypertrophy and aerobic capacity concurrently in young men.

Key points

• Concurrent improvements in aerobic capacity and muscle hypertrophy in response to a single mode of training have not been reported.
• In the present study, low-intensity (40% of VO2max) cycle training with BFR can elicit concurrent improvement in muscle hypertrophy and aerobic capacity.

Key words: Muscle hypertrophy, Aerobic exercise, Occlusion, Muscle strength     

Elevated Basal Levels and Exaggerated Responses to Thyrotrophin-Releasing Hormone of Prolactin and Thyrotrophin in Turner-Noonan-Syndrome

Basal levels of prolactin (Prl), FSH, LH, testosterone, estradiol, total thyroxine (T4), total triiodothyronine (T3) and thyrotrophin (TSH) were determined in four males with Turner-Noonan-Syndrome. The responsiveness of gonadotrophins to LH-RH (100 micrograms i.v.) and of Prl and TSH (200 micrograms i.v.) was studied. High basal levels and exaggerated responses to TRH of Prl were found in all patients. However no evidence of any of the well known causes of hyperprolactinaemia could be detected in them. The four men were with normal levels of T4 and T3 and showed exaggerated TSH responses to TRH. It is suggested that the alterations in Prl and TSH release are reflections of a congenital disorder in Turner-Noonan-Syndrome not yet well studied.     

Significance of Cerebral Blood Flow Analysis in the Acute Stage after Revascularization Surgery for Moyamoya Disease

Moyamoya disease is a chronic, occlusive cerebrovascular disease with unknown etiology characterized by steno-occlusive changes at the terminal portion of the internal carotid artery, either bilaterally or unilaterally, and an abnormal vascular network at the base of the brain. Surgical revascularization such as extracranial-intracranial (EC-IC) bypass is the preferred procedure for moyamoya disease. Despite the favorable long-term outcome, cerebral infarction and hyperperfusion syndrome are potential complications of this procedure, which can lead to neurological deterioration in the acute stage. In light of the similar clinical presentations between perioperative ischemia and hyperperfusion, it is essential to attempt a prompt cerebral blood flow (CBF) measurement in the acute stage after EC-IC bypass for moyamoya disease to differentiate these distinct pathologies, because the management of cerebral ischemia and hyperperfusion is contradictory to each other. Routine CBF analysis by single-photon emission computed tomography and/or magnetic resonance imaging not only facilitated a safer perioperative management but also provided important information about dynamic pathology of the hemodynamic conversion in the acute stage after revascularization surgery for moyamoya disease. We represent the current status of CBF analysis during the perioperative period of revascularization surgery for moyamoya disease, and sought to discuss its significance and efficacy to avoid surgical complications.     

Acute Hemodynamic Responses to Three Types of Hamstrings Stretching in Senior Athletes

Although stretching is recommended for fitness and health, there is little research on the effects of different stretching routines on hemodynamic responses of senior adults. It is not clear whether stretching can be considered an aerobic exercise stimulus or may be contraindicated for the elderly. The purpose of this study was to compare the effect of three stretching techniques; contract/relax proprioceptive neuromuscular facilitation (PNF), passive straight-leg raise (SLR), and static sit-and-reach (SR) on heart rate (HR) and blood pressure (BP) in senior athletes (119 participants: 65.6 ± 7.6 yrs.). Systolic blood pressure (SBP), diastolic blood pressure (DBP), mean arterial pressure (MAP) and HR measurements were taken at baseline (after 5-minutes in a supine position), 45 and 90-seconds, during the stretch, and 2-minutes after stretching. Within each stretching group, (SLR, PNF, and SR) DBP, MAP and HR at pre-test and 2-min post-stretch were lower than at 45-s and 90-s during the stretch. SLR induced smaller increases in DBP and MAP than PNF and SR, whereas PNF elicited lower HR responses than SR. In conclusion, trained senior adult athletes experienced small to moderate magnitude increases of hemodynamic responses with SLR, SR and PNF stretching, which recovered to baseline values within 2-min after stretching. Furthermore, the passive SLR induced smaller increases in BP than PNF and SR, while PNF elicited lower HR responses than SR. These increases in hemodynamic responses (HR and BP) were not of a magnitude to be clinically significant, provide an aerobic exercise stimulus or warrant concerns for most senior athletes. Key points

• Trained senior adult athletes experienced small to moderate magnitude increases of hemodynamic responses with SLR, SR and PNF stretching, recovering to baseline values within 2-min after stretching.
• Passive SLR induced smaller increases in BP than PNF and SR, while PNF elicited lower HR responses than SR.
• The elevated hemodynamic responses were not of a magnitude to be clinically significant, provide an aerobic exercise stimulus or warrant health concerns for senior athletes.

Key words: Systolic blood pressure, diastolic blood pressure, heart rate, flexibility, age     

Higher Precision of Heart Rate Compared with VO2 to Predict Exercise Intensity in Endurance-Trained Runners

The aim of the present study was to assess the precision of oxygen uptake with heart rate regression during track running in highly-trained runners. Twelve national and international level male long-distance road runners (age 30.7 ± 5.5 yrs, height 1.71 ± 0.04 m and mass 61.2 ± 5.8 kg) with a personal best on the half marathon of 62 min 37 s ± 1 min 22 s participated in the study. Each participant performed, in an all-weather synthetic track five, six min bouts at constant velocity with each bout at an increased running velocity. The starting velocity was 3.33 m·s^-1 with a 0.56 m·s^-1 increase on each subsequent bout. VO₂ and heart rate were measured during the runs and blood lactate was assessed immediately after each run. Mean peak VO₂ and mean peak heart rate were, respectively, 76.2 ± 9.7 mL·kg^-1·min^-1 and 181 ± 13 beats·min^-1. The linearity of the regressions between heart rate, running velocity and VO₂ were all very high (r > 0.99) with small standard errors of regression (i.e. Sy.x < 5% at the velocity associated with the 2 and 4 mmol·L^-1 lactate thresholds). The strong relationships between heart rate, running velocity and VO₂ found in this study show that, in highly trained runners, it is possible to have heart rate as an accurate indicator of energy demand and of the running speed. Therefore, in this subject cohort it may be unnecessary to use VO₂ to track changes in the subjects’ running economy during training periods.

Key points

• Heart rate is used in the control of exercise intensity in endurance sports.
• However, few studies have quantified the precision of its relationship with oxygen uptake in highly trained runners.
• We evaluated twelve elite half-marathon runners during track running at various intensities and established three regressions: oxygen uptake / heart rate; heart rate / running velocity and oxygen uptake / running velocity.
• The three regressions presented, respectively, imprecision of 4,2%, 2,75% and 4,5% at the velocity associated with the 4 mmol·L^-1 threshold.
• The results of the present study show that, in highly trained runners, it is possible to use heart rate as an accurate index of the external work rate during sub maximal running speeds.

Key words: Running velocity, internal load, relationships, standard error     

心脏手术深低温低流量灌注不同血气管理对婴幼儿脑保护的影响

目的：在深低温低流量灌注模式下,观察快速降温期应用不同稳态血气管理对婴幼儿脑功能的影响,方法：将20例法洛四联症患者承机分为pH稳态和alpha稳态两组,并根据不同的时间点,低流量和血气管理方案随机平均分层析因分析,脑功能监测为：生化指标,脑温,脑电图,智商等。结果：应用pH稳态的脑部温差较应用alpha稳态低;复温末alpha稳态组中低流量25ml/kg.min^-1乳酸含量最高,术后24小时alpha稳态组中低流量25ml/kg.min^-1神经元特异性烯醇含量最高,术后4天脑电图和术后2个月智商两种稳态组间判别无显著性意义。结论：深低温低流量注快速降温期应用PH稳态进行血气管理可起到更好的脑保护作用。     

Effects of Antecedent GABA A Receptor Activation on Counterregulatory Responses to Exercise in Healthy Man

The aim of this study was to determine whether antecedent stimulation of γ-aminobutyric acid (GABA) A receptors with the benzodiazepine alprazolam can blunt physiologic responses during next-day moderate (90 min) exercise in healthy man. Thirty-one healthy individuals (16 male/15 female aged 28 ± 1 year, BMI 23 ± 3 kg/m²) were studied during separate, 2-day protocols. Day 1 consisted of morning and afternoon 2-h hyperinsulinemic-euglycemic or hypoglycemic clamps with or without 1 mg alprazolam given 30 min before a clamp. Day 2 consisted of 90-min euglycemic cycling exercise at 50% VO_2max. Despite similar euglycemia (5.3 ± 0.1 mmol/L) and insulinemia (46 ± 6 pmol/L) during day 2 exercise studies, GABA A activation with alprazolam during day 1 euglycemia resulted in significant blunting of plasma epinephrine, norepinephrine, glucagon, cortisol, and growth hormone responses. Lipolysis (glycerol, nonesterified fatty acids) and endogenous glucose production during exercise were also reduced, and glucose infusion rates were increased following prior euglycemia with alprazolam. Prior hypoglycemia with alprazolam resulted in further reduction of glucagon and cortisol responses during exercise. We conclude that prior activation of GABA A pathways can play a significant role in blunting key autonomous nervous system, neuroendocrine, and metabolic physiologic responses during next-day exercise in healthy man.